LOCUS       NZ_CP063765             5596 bp    DNA     circular CON 28-NOV-2023
DEFINITION  Klebsiella pneumoniae strain FRPDR plasmid pFRPDR_6, complete
            sequence.
ACCESSION   NZ_CP063765
VERSION     NZ_CP063765.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN16451036
            Assembly: GCF_015243235.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 5596)
  AUTHORS   sun,B. and He,Z.
  TITLE     Comparative Genomic Analyses of Polymyxin-Resistant Klebsiella
            pneumonia Clinical Strains from China
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5596)
  AUTHORS   sun,B. and He,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-OCT-2020) School of Life Science, University of
            Science and Technology of China, 1129 Huizhou Ave, Hefei, Anhui
            230052, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.9b
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 15.66x
            Sequencing Technology             :: Complete Genomics
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 11/28/2023 11:37:45
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,089
            CDSs (total)                      :: 5,966
            Genes (coding)                    :: 5,779
            CDSs (with protein)               :: 5,779
            Genes (RNA)                       :: 123
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 187
            CDSs (without protein)            :: 187
            Pseudo Genes (ambiguous residues) :: 0 of 187
            Pseudo Genes (frameshifted)       :: 70 of 187
            Pseudo Genes (incomplete)         :: 126 of 187
            Pseudo Genes (internal stop)      :: 24 of 187
            Pseudo Genes (multiple problems)  :: 33 of 187
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP063765.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5596
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="FRPDR"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pFRPDR_6"
                     /geo_loc_name="China: Anhui"
                     /lat_lon="31.85283 N 117.260044 E"
                     /collection_date="2020-05-06"
                     /collected_by="Zhao Liping"
     gene            17..262
                     /locus_tag="IM727_RS30650"
     CDS             17..262
                     /locus_tag="IM727_RS30650"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032433971.1"
                     /translation="MQPQSPVHHGLPRIDARTVCPVVVRLETPAGQQFRVRYQKIQFQP
                     PLVPVFNPQHAVLVFIESGHQNPLEARHQLFTLAGR"
                     /name="hypothetical protein"
     gene            complement(444..788)
                     /locus_tag="IM727_RS30195"
     CDS             complement(444..788)
                     /locus_tag="IM727_RS30195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006122514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_062955149.1"
                     /translation="MAVKRTRFLGIRVTDGEYQQLLERCNGRQLAVWMRETCLDTRPAR
                     SLRLPSIDPVLLRQLAGMGNNLNQIARKINGGQWSGADRVQVVAALMAIDAELERLRHA
                     VLEKGADDDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            complement(778..954)
                     /locus_tag="IM727_RS30200"
     CDS             complement(778..954)
                     /locus_tag="IM727_RS30200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004197757.1"
                     /translation="MQKPKKKPLVVKKRSWHPAVMANLMRQAQEEDYRREQMAELERSA
                     RSHFADIGGEDGG"
                     /name="hypothetical protein"
     gene            1594..1752
                     /locus_tag="IM727_RS30775"
     CDS             1594..1752
                     /locus_tag="IM727_RS30775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_071596352.1"
                     /translation="MHEPPVQPDCCALSGNYHLESNPDGHAKTPLAAAIGNWIYTREIL
                     RGLEVGA"
                     /name="replication initiation protein"
     gene            2110..2436
                     /locus_tag="IM727_RS30205"
     CDS             2110..2436
                     /locus_tag="IM727_RS30205"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_050516389.1"
                     /translation="MRERTNIMLDIGLKQSLQRLSNKTKMSLSDLINQRLSGSLSVEER
                     IGEKPEWMRNLDPYLANHFSNIELPLPSPLKEYQEKMDEIDSIINDMMVLKEYFRAKFY
                     DEIK"
                     /name="hypothetical protein"
     gene            2462..3007
                     /locus_tag="IM727_RS30210"
     CDS             2462..3007
                     /locus_tag="IM727_RS30210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_023317533.1"
                     /translation="MFFKRYLTEKEIYKAALPIVRLQGRVNTSLRFLSKYSDVNKNGLI
                     ALHLNLLHFISYNIHGVESLKITQAGINKAVQQSPHADEIKNIAQAIGRKGPEYIEGLI
                     RNPWGKKNDNPLIFIMSIEESIKETLNSGYMLYRVAPITEGEAAAFMDVLKQLLLFEDN
                     FRLFGRDLVYKIEKLKIR"
                     /name="hypothetical protein"
     gene            3073..3423
                     /locus_tag="IM727_RS30215"
     CDS             3073..3423
                     /locus_tag="IM727_RS30215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004197756.1"
                     /translation="MYWLIAFLAFIFPGRSFADGYTAYYRCNGTQEASLTVKDNKLSMV
                     VGLSKFNRQLDTYRFKEKGIEVVMQRAASDDNSEAIMSFIMPDVNNMTLLVKNNGYIIV
                     KTTCWLTKTDKQ"
                     /name="hypothetical protein"
     gene            complement(3493..3759)
                     /locus_tag="IM727_RS30220"
     CDS             complement(3493..3759)
                     /locus_tag="IM727_RS30220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262326.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_004197762.1"
                     /translation="MVWTINYSDRALKSLRKMDKQNARRIVDFMSLRIAVAADPRQSGK
                     PLKGELGEFWRYRVGDYRVLCEIRDDELVILAATIGHRREVYD"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(3743..3967)
                     /locus_tag="IM727_RS30225"
     CDS             complement(3743..3967)
                     /locus_tag="IM727_RS30225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000879771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6290 family protein"
                     /protein_id="WP_000879771.1"
                     /translation="MLAIRLSDEIESRLDSLAKQTGRTKTFYAREAILAHLEDLEDYYL
                     SAETAARVRRGDEAVHSSEDVRKSLGLDD"
                     /name="DUF6290 family protein"
     gene            complement(4634..4846)
                     /locus_tag="IM727_RS30230"
     CDS             complement(4634..4846)
                     /locus_tag="IM727_RS30230"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_021527181.1"
                     /translation="MTELEKQLLSALEQLQQDYSKRLDEWESAFGEWRKMSGLMQRENA
                     ALSERVTRLSQQVERLSGQLQRLSR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(4853..5362)
                     /locus_tag="IM727_RS30235"
     CDS             complement(4853..5362)
                     /locus_tag="IM727_RS30235"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_032433972.1"
                     /translation="MNSLLTLAKDLEQKSKAQQQSTGEMLKAAFSEHEKSVRAELSESG
                     KRISAAIRAHEQGMSAAMQSNRLSVMRMVGRTWLTITMVSGLLFASLSGVLWYQGSLIA
                     SNLAEIDRQNAALSKLNAKTWGVTYLEDSNGRFLVLPKGTAVDRTQSWTVGNGRSKQNA
                     LRLVKE"
                     /name="MbeB family mobilization protein"
     Typing          complement(1041..1117)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="91.25"
                     /coverage="98.0"
                     /evalue="1.04e-21"
     Typing          1306..1703
                     /name="rep_cluster_2370"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="89.526"
                     /coverage="91.0"
                     /evalue="3.03e-139"
     Typing          complement(3..454)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="99.0"
                     /evalue="1.82e-93"
     Typing          complement(4519..5594)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.721"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 ccggtgcgtt cgggtcgtgc agccccagtc gcccgttcac cacggtctgc cacgcatcga
       61 tgcgcggacg gtctgcccgg tcgtagtacg gctggagacg cctgccggtc agcagttccg
      121 tgttcggtac cagaaaattc agttccagcc gccccttgtc ccggtgttca acccacagca
      181 cgctgtactg gtctttatcg agtccgggca tcagaacccg ctcgaagctc gccatcagct
      241 tttcacgctg gccgggcggt aaatcctgtt cagcaaaaga caggacgccg gaggtgtact
      301 ttttggcgta gggcgaggcg tcgataagct cccggacctc ctccggcttt ccctgcagga
      361 cgatggcccc gtcgcgctgg cggtctttcc ccagcagata atccaccggc ccggcaccgc
      421 cgcctcgccc tcgcggatga aacttaacga tcatcgtctg cccccttttc cagcacggca
      481 tgccgcagcc gctcgagttc ggcatcaatg gccatcagcg cggccaccac ctgaacccgg
      541 tcagcgcctg accactggcc gccgttaatt ttccgggcga tctggttgag gttgttcccc
      601 atcccggcaa gctggcgcag caaaaccgga tcgatggacg gcagccgcaa cgaccgcgcc
      661 gggcgcgtat cgagacaggt ttcccgcatc cacaccgcca gttgccttcc attgcagcgc
      721 tccagcagct gctggtactc cccatcggtc acccggatac ctaaaaaacg ggtacgctta
      781 accgccatcc tctcctccga tatccgcaaa atgtgaccgg gctgaacgct caagctctgc
      841 catctgctcc cggcggtaat cttcctcctg tgcctgacgc atcagatttg ccatcactgc
      901 cggatgccag gatctttttt ttacgacgag cggcttcttc ttcggcttct gcattatccc
      961 tccttcgcaa acgccggacg gcacggaaaa gttttccggg tcgctccggc gttggacaag
     1021 cgccagcgcg tcagtggtgt tagcgggttt cggggcgcag ccctgaacca gtcacagcgt
     1081 ttacgctgtg tatactggct tactatgttg gcatgagagc acgattcatg aaagtgcctc
     1141 atgtggcagg tgtgatatgt ggctcaggag cgtccgcaat accatgcacg gacagatgaa
     1201 atgacgcctc ctcgctcact cggtagctgc gctcctgccg ttcggctgcg gcgggcgtga
     1261 ccgactcacg gataacggga taaagcaaag gcgctcaaat cagaaagggc cggagaaaag
     1321 gatgcggcgt tgccgttttt ccataggctc cgcctcccct gacgagcatc acagaaatgg
     1381 acgctcaagt cagaggagga cgaaacccga caggacttaa agatgccggt gatttctctc
     1441 cggcggctcc ctcgtgcgct ctcctgttcc gaccctgccg tttaccggat acctgttccg
     1501 cctttttccc tttcgggaag cgtggcgctt tctcatagct cacgctgctg gtatctcggc
     1561 tcggtttagg tcgttcgctc caagctgggc tgtatgcacg aaccccccgt tcagcccgac
     1621 tgctgcgccc tttccggtaa ctatcatctt gagtccaacc cggacggaca cgcaaaaacg
     1681 ccactggcag cagccattgg taactggata tatacgagag agatattgag aggtcttgaa
     1741 gtgggtgcct aactgcggct acactgaaag aacagttttg gtatctgcgg tctcgtatac
     1801 ccagttaccc ggttaagcag ttccccaact gacttaacct tcgatcaaac cgcctcccca
     1861 ggcggttttt tcgtttacag tgcaagagat tacgcgcaga aaaaaaggat ctcaagaaga
     1921 tcctttaaat gatcgtcagt tctgttatcc ccggagctcc atccatccca cggaaaaaca
     1981 cccgccactg atgccctcag cgcactgcac atcatgtaca aaccactccc tccctccttt
     2041 tttattgcgt ttcttaatgc gcaatggtag ctttgtgcgc aacaaaaaac gcaatagcaa
     2101 gggggggtaa tgagagaaag aacaaatatc atgctagata taggattaaa gcaatcctta
     2161 caacggcttt cgaataaaac caaaatgagt ctaagtgatc ttattaacca gagattatca
     2221 ggttcacttt cagttgaaga acgcattggc gaaaagcctg aatggatgag aaaccttgat
     2281 ccctatctcg ccaatcactt ttcaaatatt gagttaccgc tcccttcacc attaaaagaa
     2341 taccaagaaa aaatggatga gatagattca attattaatg acatgatggt tctaaaggaa
     2401 tatttcagag ctaagtttta cgacgaaata aaataggcac gctatacgga ggcggagaat
     2461 tatgtttttt aaacggtatt taacggagaa ggaaatatac aaggcagcac tcccaattgt
     2521 taggcttcaa gggagagtta acacttcatt acgtttttta tccaaatact cagatgtaaa
     2581 caaaaatgga ttaattgcat tgcatcttaa tttattacat tttatctcat acaacataca
     2641 cggtgtagag tctctgaaaa tcactcaagc aggcataaac aaagctgtac aacaaagccc
     2701 tcacgcagat gaaataaaaa atattgcgca ggcaatagga cgcaaaggac ctgagtacat
     2761 cgaaggatta attagaaacc catgggggaa gaaaaatgat aatccactaa tatttataat
     2821 gagcattgaa gaaagtataa aagaaaccct taactcaggt tatatgttat atagagtagc
     2881 tcccataact gagggtgaag cagctgcttt catggatgtt cttaaacaat tattattatt
     2941 tgaggataat tttcgccttt ttggtagaga cttggtttat aaaatagaaa aattaaaaat
     3001 cagataacaa agtatggtga acgaaaaatg aaagagagga tagtgaaatg tgtaatacca
     3061 agattctcaa ctatgtattg gttaattgcg ttcctagcat ttatattccc cggtcgatct
     3121 tttgctgacg gttacaccgc ttactaccga tgtaatggta ctcaggaagc atctcttact
     3181 gttaaagata acaaactaag tatggttgta ggtctttcta agtttaatag gcaactggat
     3241 acttatagat tcaaagaaaa gggaatcgaa gtagtaatgc agagagctgc ttctgatgat
     3301 aactctgagg ccataatgtc ttttattatg cctgacgtta acaatatgac cttattagtt
     3361 aaaaataacg gctacattat cgttaagaca acctgctggt taactaaaac cgacaaacag
     3421 tagaacacgt agttagtcga gtatttacca taatacctgt tgtcggcatc aaaaaagccc
     3481 gcatagcggg cttcagtcgt aaacttcgcg gcgatgtcca atcgtggcgg caaggataac
     3541 aagctcgtca tctcggatct cacacagaac gcgataatct cccacccgat agcgccagaa
     3601 ctcgcccagc tcacctttga gcggcttccc tgactggcga ggatcggcag caactgcaat
     3661 gcgtaaactc ataaaatcca caatccgtcg tgcgttctgt ttgtccatct tgcgtaacga
     3721 tttgagcgcc cgatcggaat agttaatcgt ccagaccaag tgacttcctc acgtcttcag
     3781 acgaatgcac tgcttcatca ccacggcgaa cgcgtgcagc agtttctgct gaaagataat
     3841 aatcctccag gtcttccaga tgcgccagta ttgcttcccg cgcataaaac gtctttgttc
     3901 tgccggtttg cttcgccagc gagtccagac gggactcaat ctcatcagat aaccggatag
     3961 caagcatata aacctcccga acaccatttg ctatacatgt atatcaacag taccacaatc
     4021 aacctaaaag aacaaaaaat aatcttgtta gcccctcttg ataatccgta acgcggtcaa
     4081 tatatctagc ttgagggctt tcctcttaac tattgaaagg ataacgatta tgggtcttaa
     4141 gccgggtccg aaacgaattg ccgattcaac aggggaacct gataaacgcc agcgtgacaa
     4201 taaaaaaacg ccgggaaata ctgacaaatt gaaacccagc aaatcttcaa aaaaataagt
     4261 cagtagttaa aagccctccg gggctttttt gtatctgccg ttcacgctga ttaaacataa
     4321 cacccgttgc ccgcaccgcc ccggacgggt tcgccgcgaa cggggtggca gtcaggtaaa
     4381 accggcaaca aaccacccga taaaccgccc cgttcgcgac agataaattt tacctggctg
     4441 catagccatg cagccaggtg aatgacatcc ggtttcagcg taaatgcccg atgagaaccc
     4501 tgttttcctg catttcagcc cagcgacgga ccgtcatagc ttcgctgccg ggaacgcgct
     4561 tccgtcagtt ccttttcgtg ctgccgttgc tcgtgcgccc gttctgcggc cacggccatc
     4621 tcatgacgct gcactaccgg ctcaaacgct gcagttgccc gctcaagcgt tccacctgct
     4681 gactcaagcg cgtcacgcgc tcgctcagcg ccgcgttctc ccgttgcata agccctgaca
     4741 ttttccgcca ttccccgaag gcgctctccc actcgtccag ccttttcgag tagtcctgct
     4801 gtagctgctc taatgcgctc agcaactgtt tttccagctc ggtcatcggc acttactcct
     4861 tcaccagtct cagcgcgttc tgcttcgacc gcccgttccc gaccgtccag ctctgcgtcc
     4921 tgtctacggc cgtccccttc ggcagcacca gaaaacgtcc gttgctgtct tccaggtacg
     4981 tcacgcccca ggtcttcgcg ttcagcttcg acagcgctgc gttctgccgg tcgatttcgg
     5041 ccagatttga cgctatcaag ctcccctgat accacaacac cccgctcagg ctggcgaaca
     5101 gcagtcctga caccatcgtg atggtcagcc atgtccgccc gaccatacgc atcacgctca
     5161 ggcggttcga ctgcatggcc gcgctcatac cctgctcgtg ggcgcggatg gcggcgctga
     5221 ttctcttccc gctttcgctc agttccgctc tgacagactt ctcgtgctcg ctgaatgcgg
     5281 ctttcagcat ttcgccggta ctctgctgct gcgctttcga tttctgctct aagtccttcg
     5341 ccagcgttaa aagactgttc atagatggct cccttaagtc ggatgtttcg ccctccgtcc
     5401 gggtcggcaa tgctgatgct gctctttgtg gtgcgcacga cctcaaaacc tgcgctttcc
     5461 agcgcctcag tgacgtcctg acgcgtttta agctcccctg acgaggcgag ggccagtaag
     5521 ccccgcgtaa tagcctctgc ggcttcctgc ttcgttttgg gtaacgaaga tggcgtcacc
     5581 agcgcgcgcc ggttct
//
