LOCUS       NZ_CP063746             3991 bp    DNA     circular CON 28-NOV-2023
DEFINITION  Klebsiella pneumoniae strain KP19-3023 plasmid pKP19-3023-4k,
            complete sequence.
ACCESSION   NZ_CP063746
VERSION     NZ_CP063746.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN16418109
            Assembly: GCF_015238555.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 3991)
  AUTHORS   Qin,s. and Peng,j.
  TITLE     Klebsiella Pneumoniae KP19-3023
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3991)
  AUTHORS   Qin,s. and Peng,j.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-OCT-2020) Pharmaceutical Sciences, Zhengzhou
            University, 100 Science Avenue, Zhengzhou, Henan 450001, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: CLC Genomics workbench v. 10.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 12.0x
            Sequencing Technology             :: Illumina; MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 11/28/2023 13:57:55
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,771
            CDSs (total)                      :: 5,650
            Genes (coding)                    :: 5,546
            CDSs (with protein)               :: 5,546
            Genes (RNA)                       :: 121
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 35 of 104
            Pseudo Genes (incomplete)         :: 69 of 104
            Pseudo Genes (internal stop)      :: 14 of 104
            Pseudo Genes (multiple problems)  :: 12 of 104
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP063746.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3991
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="KP19-3023"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pKP19-3023-4k"
                     /geo_loc_name="China"
                     /lat_lon="34.73 N 113.70 E"
                     /collection_date="2019-10-27"
                     /collected_by="zhengzhou university"
     gene            complement(join(3886..3991,1..317))
                     /locus_tag="IMY55_RS00005"
                     /old_locus_tag="IMY55_00005"
     CDS             complement(join(3886..3991,1..317))
                     /locus_tag="IMY55_RS00005"
                     /old_locus_tag="IMY55_00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_040214596.1"
                     /translation="MAIVSISEAARLVKVSRPTIYKMINSGKLSYTSVVKHGKAIKVID
                     TSELIRVFGSLDGVIDTVKYDVKSDAESTGINSVGLHDLQHRIALLEAENDGLKGAVKA
                     RDEHIDSLRQAMQLLEHKHEPSSPPHSPWWKFWKKS"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(317..508)
                     /locus_tag="IMY55_RS00010"
                     /old_locus_tag="IMY55_00010"
     CDS             complement(317..508)
                     /locus_tag="IMY55_RS00010"
                     /old_locus_tag="IMY55_00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_040198051.1"
                     /translation="MTKQQKTALNMAKFIQNQSLLLLDKLNELDLDVEADLCEKLHDDA
                     EHLFRTLSSRLDELQDGN"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            916..1028
                     /locus_tag="IMY55_RS28735"
                     /pseudo=""
     CDS             916..1028
                     /locus_tag="IMY55_RS28735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /name="replication initiation protein"
     gene            complement(1255..2229)
                     /locus_tag="IMY55_RS00015"
                     /old_locus_tag="IMY55_00015"
     CDS             complement(1255..2229)
                     /locus_tag="IMY55_RS00015"
                     /old_locus_tag="IMY55_00015"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_114269875.1"
                     /translation="MAFFNLLFVASLICFGGVMGSYSRGCRDSQNKFSKDKDENNKRAS
                     VYRFGIASAFICVPFISSFLHVDYSSIIFPVADGGGTKFIEQILLLISVSGISAYLGYA
                     LLDGLANKVLKEQVDGIDKKQQDLEAEQDEFKDELDRSKELIEQLEMDKKTTKFELGYF
                     KAISAVDKAESMMSIPDEALSVKKKLTEALDAVTESLSLVKREDVAKDDYDKLLVLKAY
                     ILKRLDRIDDALSITDELLMSNEDNPILIYNKACYQYILRRCQADNSDIKDMIRRALTI
                     KVTDPEFIRRQEKIRTKVIGNKDNDLEGLFTDAELEELKVAIK"
                     /name="hypothetical protein"
     gene            complement(2810..3082)
                     /locus_tag="IMY55_RS00020"
                     /old_locus_tag="IMY55_00020"
     CDS             complement(2810..3082)
                     /locus_tag="IMY55_RS00020"
                     /old_locus_tag="IMY55_00020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004897311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_019405140.1"
                     /translation="MATDAQVKEINALIKKYPDSCSICHEVYDEDDVTYTVFGYDRKGK
                     IQVTTGCCAGMLTEPVLLGVCGCFDPEERDEIMQNHPMAKQFFTE"
                     /name="hypothetical protein"
     Typing          557..1028
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="86.777"
                     /coverage="92.0"
                     /evalue="7.8799999999999974e-146"
ORIGIN
        1 tctcgagcct taacagcccc tttaagacca tcgttttcag cttcaagtaa agcgattcta
       61 tgttgtaaat catgtaaacc aacgctgtta atacctgtag attctgcgtc actttttaca
      121 tcatatttta cagtatcgat tacaccatca agagaaccaa acactctaat tagctctgaa
      181 gtatcaataa ctttaatagc tttcccgtgc tttaccacag aggtgtaact taacttccca
      241 ctgttaatca tcttgtaaat tgtggggcga ctaaccttaa caagccgtgc cgcttcacta
      301 atagacacta tggccatcag ttaccatcct gtaattcatc cagtctcgag gacagtgtac
      361 ggaaaagatg ctccgcatca tcatggagtt tctcacaaag gtcggcctcg acatcgagat
      421 caagctcgtt aagcttatcc agcaggagca gagactgatt ctggatgaat ttagccatgt
      481 tcagcgctgt tttctgttgt tttgtcataa gaagcctcag agcgtcagtg ctcaatgtca
      541 aactagtctc ttttccgctt cctcgctcac tcagtcgcta cgctccagcc gttcggctgc
      601 ggcgagcggc aggagcgtgt acataaaaac gtaggcaacc aagatatgag gcagaaaagc
      661 aaaggccgcc gaacggctgt ttttccatag gctccgcccc cctgacaagc atcgcggaaa
      721 cggacgctca aatcagtggt ggcgaaaccc gacaggactt aaagatacca ggcgtttccc
      781 cctggtggct ccctcgtgcg ctctcctgtt cctgcctttc ggtttaccgg tgtcattccg
      841 ctgttatggc cgcgttttct cattccatgc ctgatactcg gctccgggaa ggcagttcgc
      901 tccaagctgg actgtatgca cgaacccccc gttcagtccg actgctgcgc ccgttccagt
      961 aactatctct tgagtccaac ccggaaagac acgacttatc gccattggca gcagccactg
     1021 gtaattgatt tagaggagtt agtcttgaag ttatgcgcca gttaaggcta aactaaacga
     1081 actgttttgg tgactgcgct cctctaagcc agttacctcg gttcagaaac ttgccggtta
     1141 ctgaaccttc gaaaaaccac cttgccgggt ggttttttcg tttgaggggt agagtacgct
     1201 gcacctcaaa ggatcgtcaa ggatcttttt ggaattcata gagctagcca ctgcctattt
     1261 gatagccacc tttaattcct ccagctcagc gtcagtaaac aacccttcaa gatcattatc
     1321 tttatttcct atgaccttgg ttcttatttt ttcttgcctg cgaatgaatt caggatctgt
     1381 aactttaata gtcagagccc tgcgaatcat atccttaata tcggagttat cagcttggca
     1441 acgacgcaaa atatattgat agcaagcctt attgtaaata agtatagggt tatcctcatt
     1501 actcataagt aactcatccg taattgacag agcatcatca atacgatcaa gtcttttcaa
     1561 aatataagct ttaagcacta agagcttgtc gtagtcatcc ttagctacat cctctctttt
     1621 aacaagagac aatgattccg ttacagcatc taatgcctca gttagttttt tcttaacaga
     1681 aagagcctca tccggaatgg acatcattga ttctgcttta tccacagctg aaatggcttt
     1741 aaagtatcct agctcgaatt ttgttgtttt cttatccatt tcaagctgtt ctatcagctc
     1801 cttgctccta tcaagctcat ctttaaactc atcttgttca gcctccaaat cttgttgctt
     1861 tttatctata ccgtctactt gctcttttaa tactttgttg gcaagaccat ccagtaatgc
     1921 gtacccaaga tacgcagaga tccccgaaac agaaataaga agcaatattt gttctatgaa
     1981 cttagttcca ccaccatcag ccacaggaaa tattatactt gagtaatcaa catgaagaaa
     2041 tgatgatatg aaaggaacac aaataaatgc ggatgctata ccaaatctat aaacactagc
     2101 tcttttatta ttctcatcct tatcttttga aaatttattt tgggagtctc ggcaacctct
     2161 ggaatagctc cccataacac caccaaaaca aattagagat gcaacaaaca ataggttaaa
     2221 aaaagccatg agagaatcct tgcgcaaagc aaacaataaa actttgttca ggtaagttaa
     2281 gataagctca atcatatact aaccaataac aaaaaaggca gccttggctg cctttttctg
     2341 ctgacttgac acttacaagt ctggacgctc atcgtgaagt ttgatttttt tcatagagca
     2401 cctctcatta attgttcctt tgcaagccca tgcttgctta gcgctgaata tagggcataa
     2461 acccagcact cacaacaaca aaaatgctta aaatgataaa atcaacccat gtattgacat
     2521 tgggtgaaaa aaaaagtata atgcactggt agccaaaggg gcggggcttc gcttattcct
     2581 taggaataag cgaagccccg cccctttggc taccataatt aaatgattga atttaaagga
     2641 tttttttaca ataacatcct tcctctcaat agtcatataa acatgcccca tgtattcgtc
     2701 attctggatt tttcaaaaga atgaatgcgt gcatttacca taacgcctca tttcacgcag
     2761 atatgaaaaa gcccgcacaa ggcgggcttc agactcaaca gatacggaat cactctgtga
     2821 aaaactgctt tgccatgggg tgattctgca tgatctcgtc acgttcttcc gggtcaaagc
     2881 acccacagac acccagcaga accggctctg tcagcattcc ggcacagcat cccgtggtga
     2941 cctgtatttt acctttccgg tcatacccaa aaacggtgta ggtcacatca tcctcatcat
     3001 agacttcatg acagatggag catgagtccg ggtatttttt gatgagtgca ttaatttctt
     3061 ttacctgagc gtcagtagcc aaaacatctc cctgagtcac tgattatcac aatccgctga
     3121 ctctaacaca gactcaggca tccgccgttg atttaccata acgtcccatt acacgcaccg
     3181 ctaaaacggc ctcagaacga ttctggggcg taacccatta aatcctacgt cttaccccct
     3241 gaaaacgcct caaaatcgct ccagggcgtt ttacgggcat gaatcgttat gcacttgact
     3301 gactgtctgg ggatttcagc ctgaattcag gccaggcaac tcaaaacccg caccaaacca
     3361 gactgaaaac ggtgaacggg tgcgcaattt attgcgtggc cgtgaagcgt tgccggcgca
     3421 gccggaaaac cggttttcag cacgttcagg aatcgggttt gtcgagacca gcaatcacat
     3481 tatggcaagt cgccggacga ctcaccacac gattcaccgg cgattcgact cgtgacttga
     3541 ctctagagtc gccatggtca aaaagccacc aacccggcaa gtcgactcta gagtcaagtc
     3601 atcataacgt actgattata tggcgtgatt cacatatgaa tcgtgggcaa gtcatcggcg
     3661 attcgccata gagtcgtcca cgactcggat tcaccttaca aaaaataaaa ttgcgtaatt
     3721 taaaatagat aactgattga aaataaaggt ctatctgcaa aattttaatt ttgcgtagtg
     3781 tgtgggtatt tttagtggtg aggagcgttc agggatctga cgatttttgg gaaggttcag
     3841 gaactccccg gaatggccgg agagcaaaat ggaagcatgg cgggatcagg attttttcca
     3901 gaatttccac cacggggaat ggggtggtga agaaggttcg tgcttgtgct ccagaagctg
     3961 catagcctga cgaagggaat caatatgctc a
//
