LOCUS       NZ_CP063524             1580 bp    DNA     circular CON 17-JUN-2023
DEFINITION  Escherichia coli strain EH01-18-02 plasmid pEH01-18-02_6, complete
            sequence.
ACCESSION   NZ_CP063524
VERSION     NZ_CP063524.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN16387489
            Assembly: GCF_016944775.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1580)
  AUTHORS   Moser,A.I., Campos-Madueno,E.I. and Endimiani,A.
  TITLE     Complete genome and plasmid sequences of MDR Escherichia coli from
            local people and environmental sources in Laos
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1580)
  AUTHORS   Moser,A.I., Campos-Madueno,E.I. and Endimiani,A.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-OCT-2020) Institute for Infectious Diseases,
            University of Bern, Friedbuehlstrasse 51, Bern 3001, Switzerland
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Flye v. 2.7.1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 983x
            Sequencing Technology :: Illumina NovaSeq; Oxford Nanopore MiniION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 06/17/2023 06:20:24
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,803
            CDSs (total)                      :: 4,682
            Genes (coding)                    :: 4,484
            CDSs (with protein)               :: 4,484
            Genes (RNA)                       :: 121
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 198
            CDSs (without protein)            :: 198
            Pseudo Genes (ambiguous residues) :: 0 of 198
            Pseudo Genes (frameshifted)       :: 77 of 198
            Pseudo Genes (incomplete)         :: 123 of 198
            Pseudo Genes (internal stop)      :: 41 of 198
            Pseudo Genes (multiple problems)  :: 38 of 198
            Pseudo Genes (short protein)      :: 2 of 198
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP063524.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1580
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="EH01-18-02"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pEH01-18-02_6"
                     /geo_loc_name="Laos"
                     /collection_date="2018"
                     /collected_by="Institute for Infectious Diseases Bern"
     gene            complement(485..961)
                     /locus_tag="IQS16_RS23740"
                     /old_locus_tag="IQS16_23730"
     CDS             complement(485..961)
                     /locus_tag="IQS16_RS23740"
                     /old_locus_tag="IQS16_23730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000469298.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_000469298.1"
                     /translation="MFIDSEKRLKQLSDEAKKNTEDLEEAKKNSRFTQVSPKGWERVRE
                     LLKDSQGISALKLYSFLAEHIDPTCGAVVADQQFLAEKLGVSRSTIIRWLNYLESKNAL
                     VRIPVAGKVCAYALDPHEVWKGYNTTKNHAAFVTKTLVNKDGDIQRRIMAMFSN"
                     /name="helix-turn-helix domain-containing protein"
     Typing          1041..1149
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="1.95e-50"
     Typing          complement(1352..1444)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="90.426"
                     /coverage="100.0"
                     /evalue="4.39e-26"
     Typing          2..255
                     /name="Col(MG828)"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="93.333"
                     /coverage="97.0"
                     /evalue="3.08e-102"
ORIGIN
        1 gcatgttgtc ggtgattccg ctgttgttct ttcggggatg agtcgtcaga tagcccttca
       61 aacgcgctca gaagctctca gacgcacgag gcggggttta aatgcccgtc agggcatgga
      121 aggcgcgaaa aacgcttaca gagcgtttca ggaggtcagg cgcgccgata cccctagcca
      181 ggggctttca ggtcgccagg aaccgcgcca gccccctgca aagcccattt gcacggtaca
      241 ggcagtcact cggttggtag gtggtcgtag accgtcggtg ttacagcgat gggggtctgg
      301 ggttctcccc agacggtttc cggttgcgct caaggcgctg ccggaaccgt ggcggctcgg
      361 tcgttctgga gatcggggag caaaaacacg caagggattt tgaggcggca aaagagcaat
      421 gcaggataga caatcatggt cagaacgtta acacgtagcc cctgattgtc cgcctgccgc
      481 tggctcaatt tgaaaacatg gccatgattc ggcgctgaat atcaccgtct ttgttgacca
      541 gtgttttagt gacaaatgct gcatggtttt tcgtagtgtt gtatcccttc cagacttcat
      601 gtggatcgag ggcatacgca caaaccttac cagcaacggg gattctaact aatgcatttt
      661 ttgattctaa gtaattgagc caacgaataa ttgtgcttct gctaactcca agtttttcag
      721 ctaggaattg ctgatccgca acgacagcgc cacacgtagg atcgatatgc tccgctaaaa
      781 atgagtacag cttcagtgct gatatgcctt ggctatcctt cagcagctct cgaacgcgtt
      841 cccaaccttt tggggatacc tgtgtaaacc ttgaattctt ctttgcttct tcgagatcct
      901 cggtgttttt ctttgcctca tctgaaagtt gtttcagtcg tttttctgaa tcaataaaca
      961 tgattttttc cctctaaaaa tcccatgcac taacgttggt tcaaatctaa gccatcatga
     1021 cacatctgtg caacaccttg ttgcgtgttg tcgcatcctg gatgcgacaa cgttgtgtca
     1081 ccctggatgc gacaggtaaa caaaaaacac actaaaaaaa caacaactta caaatcggcg
     1141 taaaagatct attctaggca agggtgttgc gcgcaacgca acgccctttg ggggtgtcgg
     1201 ggcgaagccc tgacccagtc acgtagcgat agcggagtgt atactggctt actcctctgc
     1261 aagcctcgcc agccgtgatt tttgccagat ttcgtggttt tcacgttcaa cagctcctgc
     1321 acgttttttg ccgtccgttc accgttaacc cccgcagaaa tcgggacagg atgtgcaaat
     1381 gtaataccgt cacccgcgac gatattccgt ttgccatctg gtcagggctt cgccccgaca
     1441 ccccgtatca gtgcgtacgt gctcaatgtg gtttccggtt ctcttccgct tcctcgccca
     1501 ctcgctcact aacgttcggt cgctggggct gcggcgagcg gtgccacctc tcgcatgttg
     1561 tcggtgattc cgctgttgtt
//
