LOCUS       NZ_CP063523             2046 bp    DNA     circular CON 17-JUN-2023
DEFINITION  Escherichia coli strain EH01-18-02 plasmid pEH01-18-02_5, complete
            sequence.
ACCESSION   NZ_CP063523
VERSION     NZ_CP063523.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN16387489
            Assembly: GCF_016944775.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2046)
  AUTHORS   Moser,A.I., Campos-Madueno,E.I. and Endimiani,A.
  TITLE     Complete genome and plasmid sequences of MDR Escherichia coli from
            local people and environmental sources in Laos
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2046)
  AUTHORS   Moser,A.I., Campos-Madueno,E.I. and Endimiani,A.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-OCT-2020) Institute for Infectious Diseases,
            University of Bern, Friedbuehlstrasse 51, Bern 3001, Switzerland
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Flye v. 2.7.1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 983x
            Sequencing Technology :: Illumina NovaSeq; Oxford Nanopore MiniION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 06/17/2023 06:20:24
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,803
            CDSs (total)                      :: 4,682
            Genes (coding)                    :: 4,484
            CDSs (with protein)               :: 4,484
            Genes (RNA)                       :: 121
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 198
            CDSs (without protein)            :: 198
            Pseudo Genes (ambiguous residues) :: 0 of 198
            Pseudo Genes (frameshifted)       :: 77 of 198
            Pseudo Genes (incomplete)         :: 123 of 198
            Pseudo Genes (internal stop)      :: 41 of 198
            Pseudo Genes (multiple problems)  :: 38 of 198
            Pseudo Genes (short protein)      :: 2 of 198
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP063523.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2046
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="EH01-18-02"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pEH01-18-02_5"
                     /geo_loc_name="Laos"
                     /collection_date="2018"
                     /collected_by="Institute for Infectious Diseases Bern"
     gene            complement(join(1482..2046,1..407))
                     /gene="mobV"
                     /locus_tag="IQS16_RS23730"
                     /old_locus_tag="IQS16_23720"
                     /pseudo=""
     CDS             complement(join(1482..2046,1..407))
                     /gene="mobV"
                     /locus_tag="IQS16_RS23730"
                     /old_locus_tag="IQS16_23720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000024818.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="MobV family relaxase"
                     /name="mobV"
     gene            986..1420
                     /locus_tag="IQS16_RS23735"
                     /old_locus_tag="IQS16_23725"
     CDS             986..1420
                     /locus_tag="IQS16_RS23735"
                     /old_locus_tag="IQS16_23725"
                     /inference="COORDINATES: protein motif:HMM:NF017542.2"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_004193480.1"
                     /translation="MSNRITQIDTATGEEVGSFVAVIRPKQKSAFERHFTMNQDALKIL
                     AKTLTGEQFKVLMMLLADLDYENYIQIAQADIADALGMQKTNVSRSVKALIDLSVILQG
                     PKVGRSYSYRLNPQFGWKGTVSNHKKALKNGLSVIQGGRT"
                     /name="helix-turn-helix domain-containing protein"
     Typing          complement(4..416)
                     /name="MOBV"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="93.478"
                     /coverage="96.0"
                     /evalue="7.44e-86"
     Typing          complement(1486..2045)
                     /name="MOBV"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="96.257"
                     /coverage="96.0"
                     /evalue="3.32e-100"
ORIGIN
        1 tcctgcgtca atggcaccac gaacgcagac aggtgcgggg tggcctcgtc ccggtgaacc
       61 accgccgcca caacacgatc cttcccgtat ttcttctcca gccattgctc agagcgggcg
      121 aaaaactccg cctgctgccg tggtgtggcc tctttccacc actcagggct tgctgtcata
      181 acgtattcaa cggccagtac cgcatcttta cggcgttttt ccggcagcag ctcccgcaca
      241 cgccccattg cctgatccgt tgatttggac acagagcagt agttttctgg tgtccgttca
      301 gcgttagcgt tcggcgtctc acgctctcgg tagcagtgct gcaacgcgct ggccacgctt
      361 cccattcccg tcagcttttt gcagcgcatt atcgcgtagt tcgccatgtt cccccctgat
      421 tccggcattc attcagccag gcgggggagc gcattgcgat acctccggcg ggccactatc
      481 aagatgatag tgtactcact agactttgct acgcaaaatc gttcgggcag caggctgcca
      541 gaaggtcaaa atcaacggcg gtctggcagg tggaaggcgt cgctccgcgt cacctcccac
      601 cactgccaag cctgttttat ttgtgcgtcg tcaagggtga aatgcactcg ctccgctcgc
      661 ccttgacgaa atcaggatct ggatatcgct tttgaaagag cacgcgccgc tcgccgcagg
      721 tcaggctgag gcgtaccgca gcgcagcgag gaacgaacag cctgaacgag gaagcggaat
      781 atcacctgaa cgggcataga aatgcttacg cacgaaccga tgaaaatagg gttcgctatc
      841 cgggcatatg agcttctcgc tataagatac agaagagagc cttctctcag cccttgtggg
      901 gcgcggctct cgcttgacga tagttatcat tattgataac gttgttatca ttattgataa
      961 ctattttttt gggagagcct cttaaatgag taaccgaatt actcagatag acactgctac
     1021 aggggaagag gtaggtagtt ttgtcgcagt tatccgaccc aaacagaagt cagcttttga
     1081 gaggcatttc actatgaacc aagacgcttt aaagatcctt gctaagaccc tcacagggga
     1141 gcaattcaaa gtcttgatga tgctgttagc tgatctcgat tatgaaaatt acatccaaat
     1201 tgcccaagcc gatattgccg acgctttagg tatgcagaaa accaatgtaa gccgttcggt
     1261 taaggcgcta attgacctat ccgtgattct tcaaggccca aaagtagggc gcagttacag
     1321 ttaccgtctg aaccctcagt ttggctggaa aggtactgtc agcaaccaca agaaagcact
     1381 aaaaaacggc ctcagcgtta tacagggcgg cagaacctaa tctggaatgc cgcaaatgac
     1441 gtcactttgc ggcgttagcc ctatcctgca tcgcatcaaa actaacgctc cataccaccc
     1501 atgccacgcg atcgcgtctg tcgctgtttt tcacgttcaa tacgctgctg tttttccttt
     1561 tcctggtttt gctgcttaaa ccgctgagcc atcatcagga tttcggtcat ctgctctctg
     1621 gaaagcccct taaaaggctc tgtgacgctc tgtaggcgtt tctgctgcgc atccatagtc
     1681 ttctgtagct cacgcgctct ttttgcgttc tgggcgctct gagaggccat agcgatggtt
     1741 cctgcaaagc catcgttcac tgcgtggctt agacgtttgg ctatggctgc ctgatcttcc
     1801 acgtctttgg tgaaaatgcc cttccgcaat acacgcggct ccagcgcctc cggcgagatc
     1861 gatacctggc tacgggtgcc acggtttatc gattcgtagt agtgctgaac tgtctgatgc
     1921 gtggcgcggc ttccctcgat gccgcgctca agacccagtt tttttactga ttcagcgtag
     1981 gtgctctgat cttcacgcat ctttgaccgc ccgccaataa actctttcgc ggacagccgt
     2041 ccatcc
//
