LOCUS       NZ_CP063520             7397 bp    DNA     circular CON 17-JUN-2023
DEFINITION  Escherichia coli strain EH01-18-02 plasmid pEH01-18-02_2, complete
            sequence.
ACCESSION   NZ_CP063520
VERSION     NZ_CP063520.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN16387489
            Assembly: GCF_016944775.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7397)
  AUTHORS   Moser,A.I., Campos-Madueno,E.I. and Endimiani,A.
  TITLE     Complete genome and plasmid sequences of MDR Escherichia coli from
            local people and environmental sources in Laos
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7397)
  AUTHORS   Moser,A.I., Campos-Madueno,E.I. and Endimiani,A.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-OCT-2020) Institute for Infectious Diseases,
            University of Bern, Friedbuehlstrasse 51, Bern 3001, Switzerland
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Flye v. 2.7.1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 983x
            Sequencing Technology :: Illumina NovaSeq; Oxford Nanopore MiniION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 06/17/2023 06:20:24
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,803
            CDSs (total)                      :: 4,682
            Genes (coding)                    :: 4,484
            CDSs (with protein)               :: 4,484
            Genes (RNA)                       :: 121
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 198
            CDSs (without protein)            :: 198
            Pseudo Genes (ambiguous residues) :: 0 of 198
            Pseudo Genes (frameshifted)       :: 77 of 198
            Pseudo Genes (incomplete)         :: 123 of 198
            Pseudo Genes (internal stop)      :: 41 of 198
            Pseudo Genes (multiple problems)  :: 38 of 198
            Pseudo Genes (short protein)      :: 2 of 198
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP063520.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..7397
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="EH01-18-02"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pEH01-18-02_2"
                     /geo_loc_name="Laos"
                     /collection_date="2018"
                     /collected_by="Institute for Infectious Diseases Bern"
     gene            complement(join(7385..7397,1..350))
                     /locus_tag="IQS16_RS23220"
                     /old_locus_tag="IQS16_23210"
     CDS             complement(join(7385..7397,1..350))
                     /locus_tag="IQS16_RS23220"
                     /old_locus_tag="IQS16_23210"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_025769092.1"
                     /translation="MAWHSVSQAQHMTGKSRRTLYRDMASGRLSWRTNASGHRELETSE
                     LIRVYGEIKNDGTAERHIVAQPDDTVPSRVMMDELRALRQEVADLKSMLLRLEHKPEKP
                     PEVAAPWWRFWQKKST"
                     /name="hypothetical protein"
     gene            710..850
                     /locus_tag="IQS16_RS23225"
                     /old_locus_tag="IQS16_23215"
     CDS             710..850
                     /locus_tag="IQS16_RS23225"
                     /old_locus_tag="IQS16_23215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_071994383.1"
                     /translation="MQEPPVQPDCCAFPGNYRLESNPGRHDKAPLAAAIGNWKFAENLF
                     S"
                     /name="replication initiation protein"
     gene            1240..1593
                     /locus_tag="IQS16_RS24110"
     CDS             1240..1593
                     /locus_tag="IQS16_RS24110"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_227664254.1"
                     /translation="MITQLKGRKIALVSFCWYELICTQMNGNQFSQEKKVKGSKPSIWS
                     ITEGEVIMATINLSLLEHDCGEAAREALLSGVPVYYADETTPENCVIKEYPDGRRELVS
                     FMTGMEKLVGANT"
                     /name="hypothetical protein"
     gene            complement(1754..2173)
                     /locus_tag="IQS16_RS23235"
                     /old_locus_tag="IQS16_23225"
     CDS             complement(1754..2173)
                     /locus_tag="IQS16_RS23235"
                     /old_locus_tag="IQS16_23225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000063058.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="WP_057109128.1"
                     /translation="MSEEQRRRGFMKAEASLRLEGIDPSGSPRYESIKARVISGEITYE
                     QGRAEILAFHTGKRAEPTTAGEMLAEEFLKPLNMSQGELAQATGIPEHHIDDIISNRRR
                     LSAEESYKLAEQFQTDKDFWLNLQALHDAWLTHQK"
                     /name="HigA family addiction module antitoxin"
     gene            complement(2316..4139)
                     /gene="mobQ"
                     /locus_tag="IQS16_RS23240"
                     /old_locus_tag="IQS16_23230"
     CDS             complement(2316..4139)
                     /gene="mobQ"
                     /locus_tag="IQS16_RS23240"
                     /old_locus_tag="IQS16_23230"
                     /inference="COORDINATES: protein motif:HMM:NF015354.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobQ family relaxase"
                     /protein_id="WP_057109127.1"
                     /translation="MAIFHLSVKTISRSAGRSATAAIAYRTGSRIVDERTGITHDYTRK
                     SGVEHSEIFLPQNSPGWASNREALWNAVEQKENRRNSTVAREFELAFPAELDGKQRLDL
                     LRDFCSSLVERHGMAVDAAIHAPGKEGDQRNYHAHVLCSTRRLSEEGFKDKTRELDDFK
                     SGEVNYWRSAWSEMTNRHLEKAGRSERIDHRSLEAQRESAISQGDSAKAAELDRVPTIH
                     IGYAAAQMEKRGIETERGNEHRQIILRNAEIQPLSRAIQLIENWRATHDNNSRAGHGSI
                     ARVPERDTGRDRRGFRTAQSNTGRAGAVPPPSARGRLRNLSELDMVRFGERSEMLLPGN
                     VSRDVEHQRTDRNQPLRRENTVTSKPKRPEVIRLEWQRLRRDKLTYVHDKAGRIAARGA
                     AQVERQRQKISSHERQRPEQPSGLIGMFKRSAYEQAASTWQTIKNGLDRRLVQLKNRLG
                     FVRGYMRKSGAYEMSTPGERLADQLAAKENPILAGQIKSLNAQKNAEDMAAYRTRVQSR
                     KQEKADMKLETIEELKQRGDARRDKADKAERAELEKASHQASLQNETIGEETKELESGE
                     KEKFSKRDQVLEKFKAKFERDKQNERGGRGR"
                     /name="mobQ"
     gene            4299..4601
                     /locus_tag="IQS16_RS23245"
                     /old_locus_tag="IQS16_23235"
     CDS             4299..4601
                     /locus_tag="IQS16_RS23245"
                     /old_locus_tag="IQS16_23235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008451932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_023139325.1"
                     /translation="MAKSRAEKLEELRQKEAQIKAQIQQLKQREQTEERKKDTRRKILI
                     GGAVLAKIKRGEWNYHQLVSLLDNELKNDRDRALFDEMGLKAVDARKESFNPGNK"
                     /name="hypothetical protein"
     gene            4649..7132
                     /locus_tag="IQS16_RS24115"
     CDS             4649..7132
                     /locus_tag="IQS16_RS24115"
                     /inference="COORDINATES: protein motif:HMM:NF014581.2"
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA transfer
                     family protein"
                     /protein_id="WP_025769090.1"
                     /translation="MKKNIQAGIFFVTMLLPVWHAAAETFTRCHPNETVWAMIQDEQLK
                     NIARLQETKRCQAVNDTYDQWRQNFQAMGDAENAIRSARDNQNWNAVKSTFDNIAPQIN
                     SLHALAEKNSSLLGAGNILALFGGDLNYLYQNAGLVAPASADGLTDNVSQMLTEPDKQV
                     KAAGIAQQLVYQTRVRAQSFIQGVAQNAGNEAQKEYREQSDKIVEDKAHNFKGDSVEGY
                     FGGFLARISQVYAPVLLFLIIVGIIGALTARRVRLDAFTASVKAVMAAVVPAVALALML
                     IFMPFLPSWVLYTAALAGTFAIYSRSKQILGGLASRCGRFPTLASGLRWMGGFVSGIDA
                     DRKQETGAASVNPASHHTHGSSRWGTAAEMRENNHLLPTEKQAGFTLARELSPPPGVDG
                     KFCFTGHVVTVAPTGSGKGIGAVIPNLLTYQGSALVFDPKGENAEVTGRRRREMGQSVH
                     VIDPFGITSGIKESVNILDRLDVTSPDCVAESAVLAESLVVSEKGGDSNHFDESARTLL
                     QGLMLHVSGLPDESRRNLAELRRLITSDEENLLALLADMAASEDVAFGIPARAANTLMG
                     MADRERGSVLSTARKNTAFLDDPRIAAALSHSDFNLSAIKAEPMTVYLVLPANRIAANT
                     RFIRLFIGSVISAVTESKAKPAHNVAFILDEFNVLGYMKSIEDAVSLLRGYGLSFWVFL
                     QDLSQLKSAYPKWQTFLANSAKTFYGTDDYDTAKYVSDTLGKSTVEFETQNSGRNSGSG
                     VSGGGGSMNRGRSSGSSQQFAGRELLTPDEVMRLGPTRPIVILKGEYPYQLTRINYLTD
                     PEYAGMADPNPYYSA"
                     /name="type IV secretory system conjugative DNA transfer
                     family protein"
     Typing          452..819
                     /name="rep_cluster_2370"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="90.566"
                     /coverage="85.0"
                     /evalue="8.49e-135"
ORIGIN
        1 ttctgccaga aacgccacca cggagccgct acttcaggcg gcttttccgg tttatgctca
       61 agccttaaca gcatagattt aaggtcagca acttcctgcc gtaaagcacg caattcatcc
      121 atcatgaccc tactcggcac tgtgtcatca ggctgtgcca cgatgtgccg ttcagctgtg
      181 ccatcgtttt taatctcgcc ataaacacgg atcagctctg aggtctcaag ttcgcgatgg
      241 ccgctagcat tagttcgcca tgacaagcgg ccagacgcca tatcccgata aagggttcta
      301 cgacttttcc ctgtcatgtg ttgtgcttgt gacacactgt gccatgccat aacccccccc
      361 ttgatgaagt gacacactct acgggctgca ctctagcagg ctaacggcat ccgggcacgc
      421 ggcaagaagg ggaaaaaaaa gcggcagacc cgtttttcca taggctccgc ccccctggcg
      481 agcatcacaa aaacagacgc tcaagccaga ggcggcgaaa cctgacagga ctataaagat
      541 catcaccgtt tccggtgaat cgctccctct tgcgctctcc tgttccgacc gtgtcgcttt
      601 accggatacc tgaccgcctt tttcccttac gggacatgtg gcgctttctc atagctcaca
      661 ctgtaggtat ctcagctcga tgtaggtcgt tcgctccaag ctgggctgta tgcaagaacc
      721 ccccgttcag cccgactgct gcgcctttcc cggtaactat cgtcttgagt ccaacccggg
      781 aagacacgac aaagcgccac tggcagcagc cattggtaac tggaagttcg cagagaattt
      841 gtttagctaa acacgcggtt gctcttgaag tgtgcgccaa agtacggcta cactggaagg
      901 acatatttgg ttgctgtgct ctgcgtaagc cagttaccac ggttcagaaa cttgccggtt
      961 tactgaacct tcgaaaaacc accttgccgg gtggtttttt cgtttacagg acaagagact
     1021 acgacgataa acaaaggatc tcaagaagat catgacaaac ccgtgaagtt gacaagtggc
     1081 atgggctcca tggggttgta ggggcaagga ggggggggga gaagtcgaaa aataaattaa
     1141 gagtcaccat gccggtcact cgcatttacc ataacatccg ttagcagaac cgcctcagag
     1201 gccctcagcg cgtttttatg ccctaagcct caaaagacca tgattaccca attaaaaggc
     1261 cgtaaaatcg cactggtgag tttttgttgg tatgaattga tatgcacaca aatgaatggc
     1321 aatcaattta gccaggaaaa gaaggtcaaa gggtcaaaac catccatttg gagtattacc
     1381 gaaggagaag tcatcatggc gaccattaat ctatcactac tggaacatga ctgcggcgaa
     1441 gccgcccgcg aagctcttct cagcggcgtt cctgtctatt acgccgatga gacgacacca
     1501 gaaaactgcg ttatcaaaga atatccggat ggccgcaggg aactggtgag cttcatgact
     1561 ggtatggaga aactcgttgg ggctaataca tgatttttgg atgtccggat ttcagaacca
     1621 ttatgacact tgaggggcag gaaaaaataa agccccgcct ggtaaggacg gggctttatt
     1681 tttaatactc tgacctgcat ggaacatcct acattagact ggaacattat atatctaatc
     1741 gcgcccctgg ctgctatttc tggtgcgtca gccatgcgtc atgcaaagcc tgcaggttga
     1801 gccagaaatc tttatcggtc tggaactgtt cggccagctt gtaagactcc tcagcactga
     1861 ggcgacggcg gttactgata atatcatcga tatgatgctc aggaattcct gtagcctgcg
     1921 ccagttcacc ctgagacatg ttcagaggtt ttaaaaattc ctctgcgagc atttcccccg
     1981 cagttgtcgg ctccgcacgc ttcccggtat ggaaagccag aatttcagct cgtccctgct
     2041 cataggttat ctcacccgaa ataactctcg ctttaatcga ctcgtagcgg gggcttccgc
     2101 tgggatctat accttccagt ctcaggcttg cctcagcctt catgaagcca cgccggcgct
     2161 gttcttcact catatctacc tcatatgtca atatcaggcg tacctcatat gtcatgaaat
     2221 actgttaacc agtacaatgc tttcagcccg aaaagacgcc tgatcagtct ggccgggtca
     2281 ggtatgcagc atggttggcg ctgtgttgca taccgctaac gcccacggcc accacgttca
     2341 ttctgcttat ccctctcgaa ttttgcttta aacttctcca gaacctgatc acgcttgctg
     2401 aatttctctt tctctccgga ctccagttct tttgtctctt cgccgatcgt ttcattctgc
     2461 agtgaagcct gatgagacgc tttttccagc tctgcacgtt cggctttgtc ggctttatct
     2521 ctccgggcat caccacgctg tttgagttct tcgatagttt ccagcttcat atcagctttc
     2581 tcctgtttgc gggactgtac gcgggtacgg tatgcggcca tatcttccgc gttcttctga
     2641 gcattgagcg attttatctg cccagccagg atcggatttt ctttcgcggc cagctggtcg
     2701 gccagccgtt ctcccggcgt agacatttcg taagcgccag acttgcgcat gtagcctctg
     2761 acaaagccaa gccgattttt cagttgcacc agacgacgat cgagaccatt tttgatggtc
     2821 tgccaggtac tggcggcctg ttcataggcg ctgcgcttaa acattcctat caggccgctg
     2881 ggctgttctg gtctttgtcg ctcatgtgag gatatttttt gtctctggcg ttcaacctgt
     2941 gccgccccac gggcggcaat ccgtccggcc ttatcgtgaa cgtaagtcag cttatcccgg
     3001 cgtaaacgtt gccattcaag ccggataacc tccggtcgtt ttggcttgct ggttacagtg
     3061 ttttcccgtc gcagaggctg atttctgtcg gttctctggt gctccacgtc acgagataca
     3121 ttgcccggca ataacatctc acttcgttct ccgaagcgaa ccatatcgag ttcggacagg
     3181 ttgcgcaggc gaccccttgc gctgggcggc gggacagctc cagcgcggcc agtgttggac
     3241 tgagcggttc ggaatcctct cctatctcgc cctgtgtctc gttcagggac tcgagcaatg
     3301 cttccatgtc ctgctctgct gttgttgtca tgggttgccc tccagttttc aatcagctga
     3361 attgcccgtg aaagcggctg gatttcagca ttacgcaata ttatctggcg atgttcattg
     3421 cctcgttcgg tctctatgcc gcgcttttcc atctgtgcag ctgcgtaacc gatatgaata
     3481 gtcggaaccc gatccagctc cgctgccttg gcggaatccc cctgagaaat agctgattcc
     3541 cgttgcgcct caagtgaacg atggtcaata cgctcggaac ggccagcttt ttccagatga
     3601 cggttcgtca tttctgacca tgcagaacgc cagtagttga cttcgccgct cttaaaatca
     3661 tcaagttcac gggtcttatc cttgaaacct tcctccgaca gtcggcgggt actgcataaa
     3721 acgtgagcat gataattacg ctggtctccc tctttacccg gagcatggat agcggcatcc
     3781 actgccattc cgtgacgctc aaccagtgaa gaacaaaaat cacgaagcag atcgagacgc
     3841 tgttttccgt caagctcggc aggaaatgcg agttcaaatt ctctggcaac ggttgaatta
     3901 cgacggtttt ctttctgttc aactgcattc cagagagcct cccgattact cgcccagcct
     3961 ggtgagtttt gagggagaaa aatctctgaa tgctcgacgc ctgatttacg ggtgtagtcg
     4021 tgggtaatcc cagttcgctc atcaacaatt cttgaacctg tacgataagc aattgccgca
     4081 gtggccgaac ggccagcact acggctgatt gtcttaacgc taagatgaaa aattgccaat
     4141 ttagtattcc tgtttttcct ttgaccttgg gtgcagggtt ttaaccctgc cgcacgcaaa
     4201 ctcgaagagt ttgcataagt gcgccctttg ctttcgcttc gggttgaaat atatcagcaa
     4261 tggagttatc gttcaaattt gaatcaaaga ggtgaataat ggcaaaatca agagcggaaa
     4321 agttggaaga gttgcgtcag aaagaagctc agataaaggc gcaaattcag caattaaagc
     4381 aaagagagca gacagaagaa cgcaagaagg atacccgacg aaaaatccta atcggtggtg
     4441 ccgtactcgc aaaaataaag cgtggggaat ggaattatca tcagctggtg agtcttctgg
     4501 acaatgagct gaaaaatgac agagaccggg cactgtttga tgaaatgggt ttaaaggcgg
     4561 ttgacgccag aaaagaatct tttaaccccg gtaataagtg acgccaatta cttattctcg
     4621 gatgatgaat cagatcagga gatttagcgt gaaaaaaaat atacaggccg ggattttctt
     4681 cgtcacaatg ctattgccag tatggcatgc agcggccgaa acattcaccc gctgccatcc
     4741 aaatgagact gtctgggcaa tgatccagga tgagcagcta aaaaacatcg ccagactaca
     4801 ggaaacaaag cggtgtcagg ccgttaatga cacttatgac cagtggcgcc aaaatttcca
     4861 ggcgatggga gatgcagaaa acgccatccg cagcgcccgc gataatcaga actggaacgc
     4921 cgtaaaatcc acgttcgata acattgctcc ccagattaat agcctgcatg cactggcaga
     4981 aaaaaattca tcgttattgg gcgcgggtaa catcctggcg cttttcggcg gagatctgaa
     5041 ttacctgtac cagaatgcag ggctcgttgc cccggccagc gctgacggac tgacagataa
     5101 cgtcagtcaa atgctgactg aaccggataa acaggtcaaa gctgcaggta tcgcgcaaca
     5161 gctggtttat cagactcggg ttcgggcaca gagttttatc caaggcgtgg cacagaacgc
     5221 cggtaacgaa gcccagaaag agtatcgaga gcaatcagat aagattgtcg aggataaagc
     5281 ccataatttt aaaggtgaca gcgtggaagg atatttcggc ggctttcttg cccggataag
     5341 ccaggtatat gctccagttc tgctgttcct tatcatcgtt ggtattattg gtgcacttac
     5401 cgcacggcgg gtaaggctgg atgctttcac ggcatcagtg aaagcagtta tggccgcagt
     5461 ggtacctgca gtagcactgg cgctgatgct gatattcatg cctttcctac catcatgggt
     5521 attgtacacg gcagcgctgg ccggaacatt cgccatttat tcccgcagta aacaaattct
     5581 tggagggctg gcctctcgct gtgggcgttt ccccacactg gcctccgggc ttcgctggat
     5641 gggcggtttt gtgtctggca tcgatgctga cagaaagcag gaaacgggcg cggcatccgt
     5701 caacccggcc tcacatcaca ctcacggctc atcacgctgg ggaaccgctg cagaaatgcg
     5761 ggaaaataac cacctgttac caacggaaaa gcaagccgga ttcaccctgg cacgagaact
     5821 gtccccaccg cccggagtgg atggaaaatt ctgtttcacc gggcacgttg tgactgttgc
     5881 ccctacgggc agcggaaaag ggattggcgc agtaattccc aatcttctga cttatcaggg
     5941 ttcagcgctt gtctttgatc caaaagggga aaacgccgag gtgacaggtc gccgccgtcg
     6001 tgaaatgggt cagagcgtcc acgtaattga cccattcggc ataaccagcg gcataaaaga
     6061 atcagtgaac attctggatc gtctcgatgt gaccagcccg gattgcgtcg ctgaatctgc
     6121 agttctggcc gaaagccttg tggtatcaga gaaaggtggt gacagtaatc attttgatga
     6181 atcagcccgg accctgttac agggcctgat gctgcatgtt tccggattac cggatgagtc
     6241 ccgacgtaat ctcgcggaac tacgtcgact gatcaccagt gatgaagaaa acctgttagc
     6301 cctgctggcc gatatggccg caagtgaaga cgttgctttc ggtatccccg cccgagcggc
     6361 caacacgctc atggggatgg cagaccggga acgaggctca gtattgtcta ccgcccgtaa
     6421 aaacacagca tttctggacg acccgcgtat agcagctgcg ctgtcacact cagacttcaa
     6481 cctgtcagcc atcaaagctg aaccgatgac agtttatctc gtgctaccag ctaatcgcat
     6541 tgcagcaaat acccgattta taaggctgtt tatcggttct gtgatctccg ctgtaacaga
     6601 atcgaaggcg aagccagctc ataatgtcgc attcatcctc gatgagttca acgtgctggg
     6661 gtatatgaaa tcaatcgagg atgccgtaag cctgttacgc ggctacgggc tgtctttctg
     6721 ggtattcctg caggatctgt cacagctgaa aagtgcttac cctaaatggc agacgtttct
     6781 ggccaactca gcgaagacgt tctacggcac agacgactat gacactgcaa aatacgtcag
     6841 cgacacccta ggtaaatcga cggttgaatt tgaaacccag aattcaggcc gcaactctgg
     6901 cagcggtgtt tccgggggtg gcggctccat gaatcggggt cgttcgtctg gatcaagtca
     6961 gcagtttgct gggagggaat tactgacacc tgacgaagtc atgaggcttg gaccaacccg
     7021 accaattgtg attctgaaag gggaataccc ttatcagctc acacgtatta actatctgac
     7081 agacccagaa tacgccggta tggcagatcc taacccctac tacagcgcat gatatttgga
     7141 atcgttgtcg gcgtactgtt agctctgatg tttggccggg tcttgctccc ggccttaacc
     7201 gccgtcgcct ttgtgatagg cataccgata tacttctctt tccgctttat ctgtgccttt
     7261 atctgggctc tggtaacgca aaacagaaga cgtagtgcta tgacaataga gacacaaaag
     7321 accacaaagc ctgaaactct accggataac gtaatcaact ttagccgcga aaaagagaaa
     7381 agagttaagt agatttt
//
