LOCUS       NZ_CP063513             2345 bp    DNA     circular CON 17-JUN-2023
DEFINITION  Escherichia coli strain EH08-18-36 plasmid pEH08-18-36_2, complete
            sequence.
ACCESSION   NZ_CP063513
VERSION     NZ_CP063513.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN16387494
            Assembly: GCF_016944735.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2345)
  AUTHORS   Moser,A.I., Campos-Madueno,E.I. and Endimiani,A.
  TITLE     Complete genome and plasmid sequences of MDR Escherichia coli from
            local people and environmental sources in Laos
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2345)
  AUTHORS   Moser,A.I., Campos-Madueno,E.I. and Endimiani,A.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-OCT-2020) Institute for Infectious Diseases,
            University of Bern, Friedbuehlstrasse 51, Bern 3001, Switzerland
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Flye v. 2.7.1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 805x
            Sequencing Technology :: Illumina NovaSeq; Oxford Nanopore MiniION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 06/17/2023 06:19:58
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,916
            CDSs (total)                      :: 4,792
            Genes (coding)                    :: 4,576
            CDSs (with protein)               :: 4,576
            Genes (RNA)                       :: 124
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 14
            Pseudo Genes (total)              :: 216
            CDSs (without protein)            :: 216
            Pseudo Genes (ambiguous residues) :: 0 of 216
            Pseudo Genes (frameshifted)       :: 66 of 216
            Pseudo Genes (incomplete)         :: 138 of 216
            Pseudo Genes (internal stop)      :: 46 of 216
            Pseudo Genes (multiple problems)  :: 35 of 216
            Pseudo Genes (short protein)      :: 4 of 216
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP063513.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2345
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="EH08-18-36"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pEH08-18-36_2"
                     /geo_loc_name="Laos"
                     /collection_date="2018"
                     /collected_by="Institute for Infectious Diseases Bern"
     gene            955..1923
                     /gene="mobV"
                     /locus_tag="IQS18_RS24250"
                     /old_locus_tag="IQS18_24260"
     CDS             955..1923
                     /gene="mobV"
                     /locus_tag="IQS18_RS24250"
                     /old_locus_tag="IQS18_24260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000024818.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobV family relaxase"
                     /protein_id="WP_025766223.1"
                     /translation="MANYAIMRCKKLTGMGSVASALQHCYRERETPNANAERTPENYCS
                     VSKSTDQAMGRVRELLPEKRRKDAVLAVEYVMTASPEWWKEATPRQQAEFFARSEQWLE
                     KKYGKDRVVAAVVHRDEATPHLSAFVVPLTQDGRLSAKEFIGGRSKMREDQSTYAESVK
                     KLGLERGIEGSRATHQTVQHYYESINRGTRSQVSISPEALEPRVLRKGIFTKDVEDQAA
                     IAKRLSHAVNDGFAGTIAMASQSAQNAKRARELQKTMDAQQKRLQSVTEPFKGLSREQM
                     TEILMMAQRFKQQNQEKEKQQRVEREKQRQMRSRGMGGMER"
                     /name="mobV"
     gene            complement(1987..2313)
                     /locus_tag="IQS18_RS24245"
                     /old_locus_tag="IQS18_24255"
     CDS             complement(1987..2313)
                     /locus_tag="IQS18_RS24245"
                     /old_locus_tag="IQS18_24255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001614210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_001070513.1"
                     /translation="MNQAALITIANELNHDQMRVLMALLAELDYENYIQVAQIDIAEAL
                     TMQKTNVSRAVKNLIDFGIILEGPKIGRSKTYRLNPQFGWKGTVSNHKKALKNGLSVIQ
                     GGRT"
                     /name="helix-turn-helix domain-containing protein"
     Typing          947..1920
                     /name="MOBV"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="94.462"
                     /coverage="96.0"
                     /evalue="0.0"
ORIGIN
        1 tgggacgaat cactgctaca aaaccaccta attcctcccc tgtactttgg tctacctgcg
       61 tgattctccg catctaaaaa tctccaattt caggaaaaat agttatctct caagataact
      121 aagttatctc tagagataac tattgtcaaa aactttccgc ttgtggcgcg gcttccgaga
      181 catccccttc tgtatcttat tactctccgt actcatacat cggcaaaatc aggcgttttt
      241 ctgcccgttc ggtgcgaacg aatttctatg tacgggaagt ggaaattccg cttcctcgct
      301 cattcgctca ctacgttcgg tcatgagtct gcggcgaaaa gtacgattag tacttgaggt
      361 gagtacgatt agtacttgag gtaagtacga ttagtacttg aggtaagtac gattagtact
      421 tgaggtgagt acgattcttc tctctagccc ttggtgtgcg cgggttcccg tatctatatc
      481 tatatattaa gggggaaaat aaaggctgaa aaacgccatt tttaaacatc gaaaaacgag
      541 cttttttgag tctggagcgt acggatttct atgtccgggg agtggaaatt ccgcttcctc
      601 gaacggacag actcgcggcg atgccgctcc ctaatccgtt cttgcggcga gcggttcctg
      661 ctctttcaaa aacgatattc attgcctgat ttcgtcaagg gcgagcgtag cgagtgccat
      721 ttacccttga cgactcaccg gataaaacag gcttggcagt ggtgggaggt gacgcggagc
      781 gacgccttcc acctgccaga ccgccgttga ttttgacctt tggaagcgca gcttcccgaa
      841 cgattttgcg aagcaaagtc tagtgagtac actatcatct tgatagtggc ccgccggagg
      901 tatcgcaatg cgctcccccg cctggctgaa tgaatgccgg aatcaggggg gaacatggcg
      961 aactacgcga taatgcgctg caaaaagctg acgggaatgg gaagcgtggc cagcgcgttg
     1021 cagcactgct accgagagcg tgagacgccg aacgctaacg ctgaacggac accagaaaac
     1081 tactgctctg tgtccaaatc aacggatcag gcaatggggc gtgtgcggga gctgctgccg
     1141 gaaaaacgcc gtaaagatgc ggtactggcc gttgaatacg ttatgacagc aagccctgag
     1201 tggtggaaag aggccacacc acggcagcag gcggagtttt tcgcccgctc tgagcaatgg
     1261 ctggagaaga aatacgggaa ggatcgtgtt gtggcggcgg tggttcaccg ggacgaggcc
     1321 accccgcacc tgtctgcgtt cgtggtgcca ttgacgcagg atggacggct gtccgcgaaa
     1381 gagtttattg gcgggcggtc aaagatgcgt gaagatcaga gcacctacgc tgaatcagta
     1441 aaaaaactgg gtcttgagcg cggcatcgag ggaagccgcg ccacgcatca gacagttcag
     1501 cactactacg aatcgataaa ccgtggcacc cgtagccagg tatcgatctc gccggaggcg
     1561 ctggagccgc gtgtattgcg gaagggcatt ttcaccaaag acgtggaaga tcaggcagcc
     1621 atagccaaac gtctaagcca cgcagtgaac gatggctttg caggaaccat cgctatggcc
     1681 tctcagagcg cccagaacgc aaaaagagcg cgtgagctac agaagactat ggatgcgcag
     1741 cagaaacgcc tacagagcgt cacagagcct tttaaggggc tttccagaga gcagatgacc
     1801 gaaatcctga tgatggctca gcggtttaag cagcaaaacc aggagaagga gaaacagcag
     1861 cgtgttgaac gtgagaaaca gcgacagatg cgatcgcgtg gcatgggcgg tatggagcgt
     1921 tagttttgat gcgatgcagg atagggctaa cgccgcaaaa tgacgccatt tgcggcattc
     1981 cagagatcag gttctgccgc cctgtataac gctgaggccg tttttcagtg cttttttgtg
     2041 gttgctgaca gtacctttcc agccgaactg gggatttagg cggtacgttt tgctccgccc
     2101 aatcttcggt ccttccagta tgatcccgaa atcaatcagg ttttttaccg cacgactgac
     2161 atttgttttt tgcatagtca gcgcttctgc aatatctatc tgcgcgacct ggatgtagtt
     2221 ctcgtaatcc agctctgcga gcaaggccat cagtacacgc atctggtcat ggtttagctc
     2281 attggcaatg gtaattagcg cggcttgatt catagtaaag tgcctctgga atgatgactt
     2341 ttgtt
//
