LOCUS       NZ_CP063510             2216 bp    DNA     circular CON 17-JUN-2023
DEFINITION  Escherichia coli strain EH09-18-41 plasmid pEH09-18-41_7, complete
            sequence.
ACCESSION   NZ_CP063510
VERSION     NZ_CP063510.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN16387495
            Assembly: GCF_016944715.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2216)
  AUTHORS   Moser,A.I., Campos-Madueno,E.I. and Endimiani,A.
  TITLE     Complete genome and plasmid sequences of MDR Escherichia coli from
            local people and environmental sources in Laos
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2216)
  AUTHORS   Moser,A.I., Campos-Madueno,E.I. and Endimiani,A.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-OCT-2020) Institute for Infectious Diseases,
            University of Bern, Friedbuehlstrasse 51, Bern 3001, Switzerland
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Flye v. 2.7.1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 397x
            Sequencing Technology :: Illumina NovaSeq; Oxford Nanopore MiniION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 06/17/2023 06:32:02
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,054
            CDSs (total)                      :: 4,930
            Genes (coding)                    :: 4,724
            CDSs (with protein)               :: 4,724
            Genes (RNA)                       :: 124
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 13
            Pseudo Genes (total)              :: 206
            CDSs (without protein)            :: 206
            Pseudo Genes (ambiguous residues) :: 0 of 206
            Pseudo Genes (frameshifted)       :: 57 of 206
            Pseudo Genes (incomplete)         :: 149 of 206
            Pseudo Genes (internal stop)      :: 32 of 206
            Pseudo Genes (multiple problems)  :: 34 of 206
            Pseudo Genes (short protein)      :: 4 of 206
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP063510.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2216
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="EH09-18-41"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pEH09-18-41_7"
                     /geo_loc_name="Laos"
                     /collection_date="2018"
                     /collected_by="Institute for Infectious Diseases Bern"
     gene            complement(371..607)
                     /locus_tag="IQS19_RS24880"
                     /old_locus_tag="IQS19_24890"
     CDS             complement(371..607)
                     /locus_tag="IQS19_RS24880"
                     /old_locus_tag="IQS19_24890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016230891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_016230891.1"
                     /translation="MTRLTNLTPAEKKFIDDAIAAAERAAGKKLNQPNRHIVLNRARAQ
                     IESQRYADRQRALREDERQQSDFAWSRPRAPRR"
                     /name="hypothetical protein"
     gene            1294..1455
                     /locus_tag="IQS19_RS24885"
                     /old_locus_tag="IQS19_24895"
     CDS             1294..1455
                     /locus_tag="IQS19_RS24885"
                     /old_locus_tag="IQS19_24895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001318243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_045343414.1"
                     /translation="MHEPPVQSDYHARSGNYQLESNPERHDKSPVAVAIGNGFRGVSLE
                     VMRLLRLN"
                     /name="hypothetical protein"
     Typing          complement(772..944)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="84.659"
                     /coverage="100.0"
                     /evalue="9.74e-41"
     Typing          891..1404
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="92.843"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 ggcactggcg gaaccaacac tccaattcca aacatcaagg cgccaatgca attcagatcg
       61 attgtgaaca atcatatcga ggaaatgaat tctaagcagc agtagcttcc tcgtagcatt
      121 acaaaaaaag ccccgtaggg ctttttttgt acctgccaat cagctggatt taccataaca
      181 cccgttgccc gcaccgcctc agaggctctc agggcgtttc tccgccctaa tacgcaaaag
      241 aacacgtatg acctcctgaa aaacgttcag aaacgctctg gtgatttttg agcgcatgaa
      301 tcgttatgca cgcgggtgaa tcgcaggtga tttcgcagcg tcagcaccac taaaccgcca
      361 aaatccgcaa tcagcggcgc ggcgcgcgcg gacgtgacca ggcaaaatcg gactgctgcc
      421 gctcatcctc acgtaatgca cgctggcggt cggcgtaacg ctgcgactca atctgcgcac
      481 gcgctctgtt cagaacgatg tgccggttag gctgattgag cttcttcccg gccgcacgtt
      541 cggcagcggc tatagcgtcg tcgataaact ttttctcagc gggggtcagg tttgtaagtc
      601 tcgtcatgtt tatctccgta tgagaatagt cccagcagtc tgccagacac agtaggaacg
      661 atgaagcgta agcgacggtg aaaggcagtc agaaacggtg aacgggagcg gattttaatc
      721 cggtgccgtg aggcgttgga caagccgcag gcgcgtcagt ggatttagcg ggtttcgggg
      781 cgcagccctg aaccagtcac gtagcgctag cggagtgtat actggcttat tatgttggca
      841 tgagtgcggt aatcaggaaa gtgcttcatg tgacagagga aaatgcagct gcgggcgtaa
      901 ggcagaaaat ggaagttcag aggatatgac gcctcctcgc tcactcagtc gcggacgctc
      961 ctgccgttcg gctgcggcgg gcgttacctg cacacggaga agagcaaagg catatgccat
     1021 gacaaacggg gcagaaaaag gatgcggcgc tgccgttttt ccataggctc cgcccccctg
     1081 acaagcatca cagaatctga cgctcaaatc agtggtggcg aaacccgaca ggactataaa
     1141 gatcccaggc gtttccccct ggtagctccc tcgtgcgctc tcctgttcct gcctttcggt
     1201 gtaccggtgt cattccgctg ttatggccgc gtttatctca ttccacgcct gacactcagt
     1261 tccgggtagg cagttcgctc caagctggac tgtatgcacg aaccccccgt tcagtccgac
     1321 taccacgccc gttccggtaa ctatcaactt gagtccaacc cggaaagaca cgacaaatcg
     1381 ccagtggcgg tagccattgg taacggattt agaggagtta gtcttgaagt tatgcgcctg
     1441 ttaaggctaa actgaaagga cagatttggt tactgcgctc ctccaagcca gttaccgcgg
     1501 ttcaaagagt tggtagctct gagaaccttc gaaaaaccgc cttgcagggc ggttttttcg
     1561 tttccagagc aagagattac gacgcaacgg acaagatctc aagaagatca tctgtgatat
     1621 tcgacagata tgggataaag gattgcaacg ccaagagtat atgagacata gcagcagcca
     1681 ccaccaccat cacagaatca agccagttgc ggcacgaaag gaaagaaccg aactgtgcga
     1741 cggtcgctac gctctccgta aattttgtat acctaccacg gagacataaa gtttctattg
     1801 ctgattcgcc ttaaaaaggg aaaaatccta ttttttctaa atggatataa ccttatggga
     1861 agctacgtag accaatcatt gacaagaaat gaatccgtaa tatctcgcgc tcaaacctcc
     1921 tggattccaa caatcatccc agtaattatc ggaatcttgt tgctgccttt ttacggctta
     1981 gggttactta taataatccc tgtgctcctt agagtttggt caacggagct tgcattaact
     2041 aaccaaagag tcatcgcaaa agtgggtttg atccgacgca atacggtgga actccgtatt
     2101 gataaagttg aaagccttgg catacatcaa gggatactgg gacgaatctt taactatggg
     2161 tctatcgtga ttaaaggcac tggcggaacc aacactccaa ttccaaacat caaggc
//
