LOCUS       NZ_CP063501            12614 bp    DNA     circular CON 17-JUN-2023
DEFINITION  Escherichia coli strain EH10-18-47 plasmid pEH10-18-47_2, complete
            sequence.
ACCESSION   NZ_CP063501
VERSION     NZ_CP063501.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN16387496
            Assembly: GCF_016944695.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12614)
  AUTHORS   Moser,A.I., Campos-Madueno,E.I. and Endimiani,A.
  TITLE     Complete genome and plasmid sequences of MDR Escherichia coli from
            local people and environmental sources in Laos
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12614)
  AUTHORS   Moser,A.I., Campos-Madueno,E.I. and Endimiani,A.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-OCT-2020) Institute for Infectious Diseases,
            University of Bern, Friedbuehlstrasse 51, Bern 3001, Switzerland
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Flye v. 2.7.1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 339x
            Sequencing Technology :: Illumina NovaSeq; Oxford Nanopore MiniION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 06/17/2023 05:56:01
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,645
            CDSs (total)                      :: 4,530
            Genes (coding)                    :: 4,300
            CDSs (with protein)               :: 4,300
            Genes (RNA)                       :: 115
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 71 of 230
            Pseudo Genes (incomplete)         :: 155 of 230
            Pseudo Genes (internal stop)      :: 31 of 230
            Pseudo Genes (multiple problems)  :: 27 of 230
            Pseudo Genes (short protein)      :: 4 of 230
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP063501.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..12614
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="EH10-18-47"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pEH10-18-47_2"
                     /geo_loc_name="Laos"
                     /collection_date="2018"
                     /collected_by="Institute for Infectious Diseases Bern"
     gene            join(12579..12614,1..156)
                     /locus_tag="IQS20_RS22335"
                     /old_locus_tag="IQS20_22345"
     CDS             join(12579..12614,1..156)
                     /locus_tag="IQS20_RS22335"
                     /old_locus_tag="IQS20_22345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008324022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_096321248.1"
                     /translation="MQIIQQTNPARYPGLSIEHGTITQHGRARAVIMNRRGIHQVSLRT
                     AEGRTVTLPVCRLFQWEA"
                     /name="hypothetical protein"
     gene            159..350
                     /locus_tag="IQS20_RS22340"
                     /old_locus_tag="IQS20_22350"
     CDS             159..350
                     /locus_tag="IQS20_RS22340"
                     /old_locus_tag="IQS20_22350"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_060634173.1"
                     /translation="MTPGRTQILKATADIAKALGTDTADVERHFLQHAVVLARLRQEGK
                     RPRVKAWMAENIGTPSAM"
                     /name="hypothetical protein"
     gene            451..801
                     /locus_tag="IQS20_RS22345"
                     /old_locus_tag="IQS20_22355"
     CDS             451..801
                     /locus_tag="IQS20_RS22345"
                     /old_locus_tag="IQS20_22355"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_060634174.1"
                     /translation="MRTKAIIAAALLATAGTAAAAPVATTYICNDGTFIHLNEQNGRLT
                     ASWHGADIPVTKIETSKLTASLNGEQGSSTLQLVVGYNTTPNPIAMITTIVNGSMNSQS
                     CKPDTASKHIDL"
                     /name="hypothetical protein"
     gene            821..1063
                     /locus_tag="IQS20_RS22350"
                     /old_locus_tag="IQS20_22360"
     CDS             821..1063
                     /locus_tag="IQS20_RS22350"
                     /old_locus_tag="IQS20_22360"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_060634175.1"
                     /translation="MAFENIERRFQRWKVIMVPGAKSYESWDEMVTGENFPEEARYKYK
                     THRTKAIDLTLKNPDYVHTMSENGITVRYRLERWR"
                     /name="hypothetical protein"
     gene            complement(1725..3284)
                     /locus_tag="IQS20_RS22355"
                     /old_locus_tag="IQS20_22365"
     CDS             complement(1725..3284)
                     /locus_tag="IQS20_RS22355"
                     /old_locus_tag="IQS20_22365"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_060634176.1"
                     /translation="MPKTKQEIATVTPITAAPSPLSLKLGDALFSVLSVSADWSGDYRA
                     QFELYGLNVKAINSAVGTAVWHAGKGRFLSVLNGSLTEFDKGDGMKLLEDSCGKFWHRT
                     DAFIARLTDLKIKTDDKVTKACIDMARAVRQAVAEFIMLRRQVAVVRLDVDMFATAPRV
                     ELVGETVTFVRPHAPYPVANADSDVVADWLVHFPQCHEFLDALVAARFASSRKNAYLFF
                     RAQSDWGKGLLFGAGGVLSRLGATVELSEGELLNILSGANSGVTASHFMGALALIVNEC
                     TRVTKKHFRLEESLALTPKYLTTQCVNLYMKIFTSADPIPGLSDTNGIDPQVANRFSML
                     DLQGDIKTRPLFLSDKGRYVDSLTSFFAAELNARVASYQAKGFDAARRDADHVLAAFTA
                     AHGLAKTAGLITDAYDEIRDDWVAFVHSRVADGHPDFLTFESSNGLVLRSPAGCWSRFL
                     EWYVDKSEPLRRARLLHDRDLIIGVAKAERDVTTGKVTKGVILPFVSGSMPDNVHRFRY
                     KN"
                     /name="hypothetical protein"
     gene            3794..4321
                     /locus_tag="IQS20_RS22360"
                     /old_locus_tag="IQS20_22370"
     CDS             3794..4321
                     /locus_tag="IQS20_RS22360"
                     /old_locus_tag="IQS20_22370"
                     /inference="COORDINATES: protein motif:HMM:NF013561.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix domain-containing protein"
                     /protein_id="WP_203161151.1"
                     /translation="MKRKASTFGLRLPPELLEQVNELAEKTNRTRTDVITDALRAYLGI
                     QELQGESGNRLDLMVELLQDIASTLKHGVPQKAVKRALAPRAENQYGSTVEARRETQRP
                     VVAEISGHYDEAEVMATIRRMREEQRDRGFRYDNKAIAQALNEAGLVQSNGRPWNNDRI
                     NTVITRRMPDLK"
                     /name="ribbon-helix-helix domain-containing protein"
     gene            4331..4822
                     /locus_tag="IQS20_RS22365"
                     /old_locus_tag="IQS20_22375"
     CDS             4331..4822
                     /locus_tag="IQS20_RS22365"
                     /old_locus_tag="IQS20_22375"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_104691663.1"
                     /translation="MAIGDDAIRDAFYVFTQQAAEMDDKGLPQEVWETPCFTYLMTKKQ
                     FNQMKVVCQRNGWDVPTSPAIPITWAMFRHVLSARNSKDKLSWQECAEILATAFSVQSN
                     VYVNRDYSEQTIVLNAVRRINVAGAGFFAMAIVDVSENNLAPVTAYHATEAKCKAIQRG
                     "
                     /name="hypothetical protein"
     gene            complement(5736..6542)
                     /locus_tag="IQS20_RS22370"
                     /old_locus_tag="IQS20_22380"
     CDS             complement(5736..6542)
                     /locus_tag="IQS20_RS22370"
                     /old_locus_tag="IQS20_22380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001751928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6694 family lipoprotein"
                     /protein_id="WP_203161152.1"
                     /translation="MKKLTGVIAFALLLTACDKPKIDASSDQSMKESIQKVRESLPADK
                     KAQFDDAVKVVAFSQINMRELMQAGTSSADVYETKIKSALEGKTGDEVINYAQTIRLER
                     EKREKEQALQEIKELEAKQTSATQAAEKMKAFKVERSRFYFQKEDYGNDQPILDISVEN
                     GTDKAVARVFFKGVIASPGRSVPWFSDVFSYKISGGLEPGEKANWELTPNRYSDWGKLK
                     VPADAIFTVTVIGLEDADGKSIYGDAEFSERDADRLNQLRDKYLSK"
                     /name="DUF6694 family lipoprotein"
     gene            complement(6648..>6890)
                     /locus_tag="IQS20_RS22375"
                     /old_locus_tag="IQS20_22385"
                     /pseudo=""
     CDS             complement(6648..>6890)
                     /locus_tag="IQS20_RS22375"
                     /old_locus_tag="IQS20_22385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221000.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /name="DDE-type integrase/transposase/recombinase"
     gene            complement(<6888..7133)
                     /locus_tag="IQS20_RS22380"
                     /old_locus_tag="IQS20_22390"
                     /pseudo=""
     CDS             complement(<6888..7133)
                     /locus_tag="IQS20_RS22380"
                     /old_locus_tag="IQS20_22390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001395480.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(7327..8256)
                     /locus_tag="IQS20_RS22385"
                     /old_locus_tag="IQS20_22395"
     CDS             complement(7327..8256)
                     /locus_tag="IQS20_RS22385"
                     /old_locus_tag="IQS20_22395"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="WP_203161147.1"
                     /translation="MLASALRDLDDLDRLAAAGAFLRSSGVAADVAAGRIAVREPTEDE
                     LAARQAHLVALRERAAAAAAKLTEARIKATGRARMLLHRAKKPGNAALYWKAKERAAER
                     LLPCPDDVKCSDWSTFLPVLGRVWWNESLLVPLYGVNESLRLECLSVEVICGRANPETA
                     GTQGEKRGLKSAPREGLFYPFDLSALRSGLPIVLCEGFMSGLALSLLIGGKLPVVCAMS
                     VGNFAATAQTLGKFVQDSPLFLVPDVGGMQLALDQGVPGAQVVDLSAVPGAEGVDGYDP
                     FDLLARHGVEMGRKYLRGLFREARDASL"
                     /name="AAA family ATPase"
     gene            complement(8475..8660)
                     /locus_tag="IQS20_RS22390"
                     /old_locus_tag="IQS20_22400"
     CDS             complement(8475..8660)
                     /locus_tag="IQS20_RS22390"
                     /old_locus_tag="IQS20_22400"
                     /inference="COORDINATES: protein motif:HMM:NF024776.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_203161148.1"
                     /translation="MKFRVSAICDLMGVSRRQLDRWIKAGTFPLPDGRDAKGYWWEGEK
                     IAPMLLKARAVKQRAE"
                     /name="hypothetical protein"
     gene            complement(9283..9837)
                     /locus_tag="IQS20_RS22395"
                     /old_locus_tag="IQS20_22405"
     CDS             complement(9283..9837)
                     /locus_tag="IQS20_RS22395"
                     /old_locus_tag="IQS20_22405"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_039022319.1"
                     /translation="MGYFDCVKLTDAEWPDHMVEGRDAALRQAMRGGVSQWCRDKVVQL
                     LPVLRCPRGEWRDAELKRVDELADALGLDDMGLFAYGAMIVKRRAYVAGSLSRAKPPEP
                     LAVVLDDGEHRCTVYAMPHPTLGVPEISERGEDGYWTISAGGCAWIKARVSLGEGVRLW
                     NELVNCARYPDAPVLTRGMLE"
                     /name="hypothetical protein"
     gene            complement(9816..10112)
                     /locus_tag="IQS20_RS22400"
                     /old_locus_tag="IQS20_22410"
     CDS             complement(9816..10112)
                     /locus_tag="IQS20_RS22400"
                     /old_locus_tag="IQS20_22410"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_039022318.1"
                     /translation="MSLYPKDKNPVFQPGGYGLRYDGQAEAIARAGEPPINYAPGQELS
                     RAAPALPEHPTHGAARAFRDHLTGEYVVAAGPVEVRTRVSAEEAALLWATLTA"
                     /name="hypothetical protein"
     gene            complement(10123..10491)
                     /locus_tag="IQS20_RS22405"
                     /old_locus_tag="IQS20_22415"
     CDS             complement(10123..10491)
                     /locus_tag="IQS20_RS22405"
                     /old_locus_tag="IQS20_22415"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_203161149.1"
                     /translation="MGRVKRLPRHLQGKAALPLLGGCFPKGHRLALLAVMPIIEARPGE
                     RHEGEAAKLALFQQLLLDGGQPPAFALHEPSLHRFGAALRSRRRGLTDAGVDKANELLD
                     QHLFSRALDAIVATKQTD"
                     /name="hypothetical protein"
     gene            complement(10479..10877)
                     /locus_tag="IQS20_RS22410"
                     /old_locus_tag="IQS20_22420"
     CDS             complement(10479..10877)
                     /locus_tag="IQS20_RS22410"
                     /old_locus_tag="IQS20_22420"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_203161150.1"
                     /translation="MSMFDQLVARLRDEQALTKSLNPGDALAAVGNGDANAPVLDQRAL
                     TIGVAPGVVACHNARLNQIADAQLLQEVRSSGAVSAADAVALDNALAASRHLPPETRAA
                     GMELVRQQLRASAAAPLSPAARGINWDE"
                     /name="hypothetical protein"
     gene            complement(10877..11563)
                     /locus_tag="IQS20_RS22415"
                     /old_locus_tag="IQS20_22425"
     CDS             complement(10877..11563)
                     /locus_tag="IQS20_RS22415"
                     /old_locus_tag="IQS20_22425"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_065945244.1"
                     /translation="MENEQIAPVDFEGANRIYLADVLAEACSALPELSDLPVPSLDGDD
                     AAVASLLHLMRQRDEMASAIGVLNFELGKLKDDAKAARARLSDAEARIAAARDALLAGN
                     EPEPRDAMLALVVDQIRADVQAADAAVDIKSREVGALDAQMNTAVHQIEGAIDELICSC
                     RIARARALELALLNELRALAVHDGDKNRFNRAFVPAPELALLSSNYGQIEMLRPGRMPH
                     LNRGVL"
                     /name="hypothetical protein"
     gene            11828..12469
                     /locus_tag="IQS20_RS22420"
                     /old_locus_tag="IQS20_22430"
     CDS             11828..12469
                     /locus_tag="IQS20_RS22420"
                     /old_locus_tag="IQS20_22430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016241263.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0000150 - DNA strand exchange activity [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="WP_072667469.1"
                     /translation="MITNTTAVAYMRASTQEQDANRAREEITLFAKEHGITIRGFYAEN
                     VSGTYLERPELNRLLEDAKPHDILLVEKLDRLSRLSAEEWSTLKERIRTKRLRIVCIDI
                     PTTLAVLQGGQPVGAGGFDVRRVVLDAMNDMLLDIAAAWARDDYETRRKRQQQGIEKAK
                     KLGRFKGKQKDEELRGKIRTLLDTSSMSQNQIAKLIGCSRNTVSRVVKGE"
                     /name="recombinase family protein"
ORIGIN
        1 ccgggcctat ccatcgagca cggcaccatc acccagcacg gacgcgcacg cgccgtcatc
       61 atgaaccgcc gaggcatcca ccaggtcagc ctcagaacgg cagaggggcg caccgtcacc
      121 cttcccgttt gccgactctt ccagtgggag gcgtgatcat gaccccaggc agaacgcaga
      181 tcctgaaagc caccgccgac atagccaaag cactcggtac cgacaccgcc gacgtagagc
      241 gccacttcct gcagcacgcc gtcgtcctcg cacggctccg gcaggagggc aagcgccccc
      301 gcgtcaaagc gtggatggca gagaacatcg gcaccccgtc agcaatgtaa gaaaaactta
      361 aacgttaagg taaaacagca tacggtttta cttacgcatt aacgaaaacc atacacccgg
      421 ggcgcgagga gcgcccccac aggacacagc atgagaacca aggccatcat cgccgcagcc
      481 cttctcgcca ccgcaggaac agccgcagcc gcaccagtcg ccacgaccta catctgcaac
      541 gacgggacat ttatccacct taacgagcag aatggcagac tcaccgccag ctggcacggt
      601 gccgacatcc ccgtcaccaa gatcgagaca agcaagctaa ccgccagcct caacggtgag
      661 cagggcagct caacactgca gctcgtcgtc gggtacaaca ccacacccaa cccgatagcc
      721 atgattacca ccatcgtcaa cggcagcatg aacagccaga gctgcaagcc agataccgca
      781 agtaagcaca tcgacctata gcaacacgaa ggagagagca atggcatttg aaaacatcga
      841 gcgccggttc cagagatgga aggtgatcat ggttcccgga gcgaagtcat acgagtcatg
      901 ggatgagatg gtcacaggcg agaacttccc cgaggaagcc cggtacaaat acaagacgca
      961 ccgcaccaag gcgatcgacc tcacgctgaa aaacccggac tacgtccaca ccatgtcgga
     1021 gaacgggatc actgtcaggt accggctcga acgctggcgc taaccccaac caggagcgcg
     1081 gcgacagctg cgcccctacc ggttagaaag cacgccccca ccagaacgca ccagagccat
     1141 tctaagcgcc tcaaccccga accaacccaa cgacacgaca gcaccccatt taagcccctg
     1201 taccccctga aagttggggc aggatttggc gaaccggtag gcgcggcgat tgccgccaaa
     1261 caccaatgca cggtgccaac cacgatccac cgtgcataaa ccacccgcca acccccgcca
     1321 gcactggaaa ctgcccccat ttccctccgg gcttttttag ctggagatcc ggcaggtgca
     1381 aaccacgcac cggggagagc gaccacaaag tgcatcccgc agggtgcagc tgcggcgcgt
     1441 caactgcgca ggaattgagc tgtaaccgcc tgtaaccgcc tgtaaccaat actgtaaccg
     1501 caaaaaagcc tttaaaatca acaatgcaac cttgtaacca ttaaaacaga aagacttatc
     1561 agaaaaaaat agaaaacagt tttcatagca gggatcgttc ggtattttcc ggttacaaag
     1621 ttgcagccag agctggcacg ggtttgcggc ggttacaagg tcggttacag acggttgcaa
     1681 gccggttaca aggcgtaaaa aaacccgccg aagcgggtta ttgtttaatt tttgtacctg
     1741 aaccggtgaa cgttgtcagg catcgaaccg gaaacgaagg ggaggatcac ccccttggtc
     1801 actttgccgg tggttacatc cctttcagcc tttgccaccc caatgatcag gtcgcggtca
     1861 tgcagcaggc gagcacggcg cagtggttcg cttttgtcca cgtaccactc gaggaaccga
     1921 ctccagcatc cagcaggtga gcggagaacg agcccgttag acgactcgaa ggtgaggaag
     1981 tcagggtgcc catcggcaac acggctatga acgaacgcaa cccagtcgtc gcggatttcg
     2041 tcatacgcat cggtgatcag cccggcggtt ttcgctaacc catgcgcggc ggtgaaagca
     2101 gccagaacat ggtcagcatc gcggcgggcg gcatcaaagc ccttcgcctg ataggacgcc
     2161 acccgagcat ttagctcggc ggcaaagaac gacgtcaggc tgtcgacata gcgtcccttg
     2221 tcggacagga acagcgggcg cgtcttgatg tcgccctgca gatcgagcat agaaaaacgg
     2281 ttggccacct gcgggtcaat gccattggta tcggacaggc cggggattgg atcggcgctg
     2341 gtgaaaattt tcatgtacag gttcacgcac tgcgtcgtga ggtacttcgg cgtcagagcc
     2401 agcgactcct caagacgaaa gtgctttttc gtcaccctgg tgcattcgtt gacgatcagc
     2461 gccagcgccc ccatgaagtg actagccgtt acaccggagt tggcaccgct caggatgttg
     2521 agcagctccc cctcggacag ctccaccgtc gcccccaggc gtgacagaac gccgccagca
     2581 ccaaacagca gccccttacc ccaatcggac tgcgcacgga aaaacaggta ggcattctta
     2641 cggcttgagg caaagcgggc tgcaaccagc gcgtcgagga actcatggca ttgagggaaa
     2701 tgaaccagcc aatcagccac cacgtcactg tcggcattag ccaccggata gggagcatga
     2761 ggacggacga acgtcacagt ttcacccacc agttcgacgc gaggagccgt ggcgaacatg
     2821 tccacgtcga ggcgaacgac tgccacctga cggcgcagca tgatgaactc agccaccgcc
     2881 tgacgaacag cccgagccat atcgatacac gccttggtta ccttgtcgtc ggtcttgatt
     2941 ttgaggtcag tcaggcgggc gataaacgca tcggtgcggt gccagaactt gccgcaggag
     3001 tcctccagca gcttcattcc gtcgccctta tcgaactcgg tcagggagcc atttaggaca
     3061 gagaggaacc gccccttgcc agcgtgccag acggcagtgc cgacggcaga gttgatagct
     3121 tttacattca ggccatacag ctcgaattga gcgcggtagt caccagacca gtccgcagac
     3181 accgacaaga cagaaaacag cgcatcgccc aattttagcg acagcggtga cggtgcagcg
     3241 gtgatcggcg ttacagtggc tatttcttgt ttagtttttg gcacgttaca actcctttaa
     3301 aaacgcagga gtcggcgtca ggcgggggct tacgagggaa attcacactt gcgcgatctt
     3361 atcgacgtga tacaattctc agcgaggatt tgagaatcac ccaacgtgat gtatgatttc
     3421 cttcgagttc gtcccgttaa gacactgact cgaaactacg gggtttaacc ccggcaatca
     3481 aagcacccgg ccaggtgctt tttttgtatc cgcagatcac ccaacacgat ccacgaacga
     3541 cgatcagctt aaccgatcct aacccttcat gccactgaac ggcgaccgct tatcgagctg
     3601 tcgtgatcgc gccgcgcttt tatccactgc aacgtcaaac tgtccacagg gcttgtccac
     3661 aggcaccaga gaggcgagcc gaagggctcg acccgtaccg attagcgaag cacggaagag
     3721 gcacgtcgaa atggacgtgg aggcgcaaaa gtgctccaat gagaggcata atcagagcac
     3781 aggagctaca cgcatgaagc gcaaagcctc gacctttggc ctacgccttc ccccagagct
     3841 actcgagcag gttaacgagc tggcagagaa aacgaaccga acccgcaccg acgtcattac
     3901 cgacgcactc cgcgcatacc ttggcataca ggagctgcaa ggggagagtg gcaaccgcct
     3961 cgacctgatg gttgagctgc tgcaggatat cgcaagcaca ctgaagcacg gtgtgcctca
     4021 aaaggccgtc aaacgcgcct tagcgccacg agcggaaaac cagtatggaa gcacggtcga
     4081 ggcgcggaga gagacacagc gccccgttgt cgcggaaatt tcggggcatt acgacgaggc
     4141 cgaggtgatg gccactatac ggcgtatgag ggaagagcag cgcgataggg gattccggta
     4201 cgacaacaaa gctatcgccc aagcgctgaa cgaagcaggg ctcgtgcagt cgaacggtcg
     4261 tccttggaac aacgaccgca tcaacacggt gatcacccga cggatgccag acttaaaata
     4321 aggaaaatca gtggctatcg gtgatgatgc tatccgggat gctttttacg ttttcacgca
     4381 gcaagctgct gaaatggatg ataaagggct ccctcaggag gtctgggaga ctccttgttt
     4441 cacttatttg atgactaaaa agcaattcaa tcagatgaag gttgtttgtc aaagaaacgg
     4501 ctgggatgtg cctacatcgc cagcaatacc aataacttgg gctatgttta ggcatgtgct
     4561 ttctgcgcgt aattcgaagg ataagttaag ttggcaggag tgtgcagaaa ttttggctac
     4621 cgcattttca gtgcagagta acgtttacgt taacagggat tactctgagc agactattgt
     4681 gctaaatgct gttcgtcgta ttaacgtcgc tggggctggg tttttcgcaa tggcgatagt
     4741 tgatgtatca gaaaacaatt tggcccccgt aactgcatac catgcaacgg aggccaaatg
     4801 taaagctata cagcgagggt gataggaagg acgcccattt aagcagttct cctcaattta
     4861 atgagaatga accatgaagc ctaaatattt cccttcattg gttcctttcg gatctagcag
     4921 catcggcggt tttctgctgt ctatcaaatt cgctattgtt caggagtttt agctccctgc
     4981 tgctgagtac agaagctatt acgcatctgt agaatgctct tgcagtacgt taaatttaat
     5041 caaatttgag cattatgtca accacaaccc cataccggta gcgcggcagc agccgcccag
     5101 ggcaaaggac tgatcgcagc cggccccgga atttttagtg cgatgtgccc tgcgcagtct
     5161 gtagtatagg ggtcagtttg gagatcggaa atttttgtac gttaagcgca ttatttaacc
     5221 aatcttggtt cgtaaggggt tgattttgcg tcagtggcta tcgcttttaa gttgtcgctc
     5281 ttgcagtatc gcatttaggt tatccgatta aggtcaaacc tctgaaaatg ccgtatagcg
     5341 cgggagtaca ccctattgcg atagcgcgat ctgttcaaaa aacaggctta acgtacaata
     5401 atttccgatc tccaaactca ccccttattc acgctggcca acctgtttcg ggcggaccaa
     5461 atgatacgtc agtgggagag atctcactaa aaactgggga taacgcctta aatggcgaag
     5521 aaacggtcta aataggctga ttcaaggcat ttacgggaga aaaaatcggc tcaaacatga
     5581 agaaatgaaa tgactgagtc agccgagaag aatttccccg cttattcgca ccttccctaa
     5641 gcgtttagca ttatttaact aatgggtatc tgccgtgatt tggtagtccc tgatatatat
     5701 tctcagggac tatgctgaag tagctttaat tttgattatt tagaaaggta cttatctcta
     5761 agttgattca gtctgtcagc atcgcgttct gaaaactcgg cgtcaccata gatcgatttc
     5821 ccatctgcgt cttcaaggcc aatgactgtg acggtaaata tagcatcagc cggaacttta
     5881 agttttcccc aatcagagta tctattaggg gttaattccc agttggcttt ctcacctggt
     5941 tccagacctc cagaaatttt atagctgaag acgtcagaga accaagggac gcttcttcct
     6001 gggctggcaa taactccttt gaagaatact ctggcaaccg ctttgtctgt tccgttttca
     6061 acgctaatat ccagaattgg ctgatcattg ccgtaatcct ctttctggaa ataaaaacgg
     6121 gaacgttcga ccttgaaggc tttcattttt tccgcagcct gtgttgcaga ggtttgtttg
     6181 gcttccagtt cttttatttc ttgcagagcc tgttctttct ctctcttctc acgctcaagg
     6241 cgaatagttt gtgcgtaatt tataacctca tcaccagttt taccttccaa cgctgactta
     6301 atttttgttt cgtagacgtc cgcagaagac gttccagcct gcattagctc tctcatgttg
     6361 atttggctaa aggcaacgac tttaactgca tcatcaaact gagctttttt atctgcaggt
     6421 aaggactctc ttactttttg aatggactct ttcatcgact gatcgcttga tgcatcgatc
     6481 ttcggtttgt cacaagcagt aagcagcaga gcaaaagcaa ttacgccagt taatttcttc
     6541 atatacctat cccaatcgta aaaaacaagg ctaatcctaa caggattagc gggcaggcac
     6601 tgttgcaaag ttagcgatga ggcagccttt tgtcttattc aaaggcctta catttcaaaa
     6661 actctgctta ccaggcgcat ttcgcccagg ggatcaccat aataaaatgc tgaggcctgg
     6721 cctttgcgta gtgcacgcat cacctcaata cctttgatgg tggcgtaagc cgtcttcatg
     6781 gatttaaatc ccagcgtggc gccgattatc cgtttcagtt tgccatgatc gcattcaatc
     6841 acgttgttcc ggtacttaat ctgtcggtgt tcaacgtcag acgggcagcg tcgggagcgc
     6901 cctgcgggta aggatttcag gccttcaata cctgagtgcg taaaccagtt aatccagcga
     6961 ccaacggatg aacgggcaca acagagagtt ctggcaacat cgctgacccg ttcaccccga
     7021 tgaagcatca gcatggccgt gagtctgcgg gcatgatttt tatcacgcgt tttatgaata
     7081 gctttctgca tcaggcgtcg ttcgccacgg ggtattggta ctatgatcgg catcgctcag
     7141 tccggttggt gattttggtt ggtttggcga ttgatcagat cgcacaatcc gggctgagtt
     7201 ccctttcagt gatctactat tccgcgaagc tatttagata acagccttta ataactatgg
     7261 gcggcactgt cataaaagcc cgccgaagcg ggctggagca caacgagaga gagaaacctg
     7321 gtaccactac aggctcgcgt cacgcgcctc ccgaaacaag ccccgcaggt acttgcgacc
     7381 catctcgacg ccgtgacgcg ccagcagatc gaacgggtcg tagccgtcca cgccctcggc
     7441 tcccggtacc gcagacaaat ccaccacctg ggcacccgga acaccctgat cgagcgccag
     7501 ctgcatcccg ccgacgtccg gcaccaggaa cagcggagag tcctgcacaa atttacccag
     7561 cgtctgcgct gttgcggcga aattccccac cgacatcgcg caaaccaccg gcaacttgcc
     7621 gccaatcagc agcgatagcg ccagcccgga catgaacccc tcgcacaaca cgatcgggag
     7681 cccactccgc agcgccgaca ggtcgaacgg gtagaacagc ccctcacgcg gcgcactctt
     7741 gagcccgcgc ttctcaccct gcgtgccagc cgtctccggg ttagcccgac cgcaaatcac
     7801 ctcgaccgaa agacactcca gacgcaggga ctcattcacc ccgtacagcg gcaccaggag
     7861 cgactcattc caccacacgc gacccagcac cggcaggaac gtgctccagt ccgagcactt
     7921 cacgtcgtca gggcacggca gcaaacgctc cgcagctcgc tccttggcct tccagtacag
     7981 cgccgcattc cccggctttt tggcgcgatg cagcagcatc cgcgccctac cggttgcctt
     8041 aatgcgagcc tccgtcagct tcgccgcagc agcagccgca cgttcccgta gtgcaaccag
     8101 atgcgcctga cgagcagcca gctcgtcctc ggtgggctca cgaacagcga tgcgcccggc
     8161 agcaacgtca gcagccaccc cggaggagcg caggaacgcc ccagccgccg ccagccggtc
     8221 gaggtcatcc aggtcacgca gcgccgacgc taacattgcc ctgtcgccct tacccgcgtt
     8281 cacaccacgc gcagaccact ccacacaggt ctccagtggg tcgagcctca gcagctcaca
     8341 ccagcacgcc agagcgcgag cctggcccag ccagcccagg actgacgtgt ctggttcaac
     8401 acgcgacaga gccgcagcga acacttccgg gtagtacagg aaattaacct cataatctcg
     8461 tctctcgttc accattattc agccctctgc ttaactgcac gcgccttaag cagcatcggc
     8521 gcaatcttct caccttccca ccagtaaccc tttgcgtcgc gcccgtcagg cagaggaaaa
     8581 gtgccagctt taatccagcg atcgagctga cgacgggaga cacccatcag gtcgcagatc
     8641 gcgcttacac gaaacttcat ggagtccctc ctgtagaaaa gccccgccgt gagtggtcgg
     8701 ggctttgagt gatgactatt gcgatgcagt ccttatgggc ttatcgccgg agcgacaggg
     8761 ctgcgccatt cgcgcttact cgcttttcac caggtggaag tgcgccaccg gaagtagacc
     8821 gaagtcccgg cacccgagcg atccgggcca tgtttatcca actgctattg gatgatgtct
     8881 gttttagact cgttttagac attttacaag ttctattttt caaagaatcc atacatggag
     8941 cggcttgcgc gtcgatttag acgcattgag ttttagacat ttaaccccaa ccgtcgtgac
     9001 gccagcatga ggaggcttac aacagagaga gataaccatg gctatcaaaa ttccggtctc
     9061 ggcacagcag cgcccaaaag agaggggctt acgccccttt tttcgtggtg gccagacggg
     9121 gagattgggg ataacgccag aaccatgccg gggaggccat gatctccgca agtcggcgct
     9181 ttctggcgtg ccctgcagca acaccgcgag cgcgtcgagc ctcgacaacg gcgggaggga
     9241 tgaccacatg ggggatcacc agacgggata cagcaaactc gctcattcca gcatcccccg
     9301 cgtcaggacc ggcgcatcag ggtaccgggc gcaattaacc agctcgttcc agagcctgac
     9361 tccctcaccg agcgagacgc gagccttgat ccatgcgcaa ccgccagcgg agatagtcca
     9421 atatccatcc tcgccgcgtt cggagatctc cggcacgccg agcgtcggat ggggcattgc
     9481 atagacagta caacgatgct cgccatcatc gaggacaaca gccagcggct cgggcggttt
     9541 agcccgggag agactaccag caacgtaagc ccgccgcttc acgatcatag cgccgtaggc
     9601 aaacagcccc atatcgtcca gccccagggc atcagcaagc tcgtctacac gtttcagctc
     9661 ggcatcacgc cactcgcccc gggggcaccg cagcaccggg agaagctgga caaccttgtc
     9721 ccggcaccat tgggagacgc caccacgcat ggcctggcga agggcggcat cccgcccctc
     9781 aaccatgtga tcaggccact ccgcgtcggt cagctttacg cagtcaaagt agcccacagc
     9841 agcgcggcct cctcagccga tacgcgggta cgaacctcga ccgggcccgc ggcgacgacg
     9901 tactcgcccg tcaggtgatc acggaacgca cgcgcagcgc catgcgtcgg gtgctcaggc
     9961 agcgcaggag cagcacggga cagctcctga cccggggcgt agttgatcgg tggctcaccg
    10021 gcacgcgcaa tggcctcggc ctgaccgtca tagcgcagac cgtagccgcc aggctgaaac
    10081 acgggatttt tgtctttcgg gtataagctc attttttaac ctctaatctg tttgtttagt
    10141 cgccacaatg gcatcaaggg cacgggagaa aaggtgctgg tcgagcagct cattggcctt
    10201 gtcgaccccc gcatcagtca atccgcgtcg cctgctcctc aacgcagcgc cgaagcgatg
    10261 cagggacggc tcatgcagcg cgaacgcggg cggttgcccc ccgtccagca acagctgctg
    10321 aaacaacgcc agctttgcag cctccccctc gtgtcgttca ccaggacgcg cctcgatgat
    10381 cggcatgaca gccagcaacg ccagccggtg ccccttcggg aagcaaccac ccagcagcgg
    10441 cagcgcagct ttgccctgca ggtggcgcgg caaccgcttt actcgtccca attgataccc
    10501 cgggcggcag ggctgagagg cgcggcagcg gaggcacgca gctgctgcct taccaattcc
    10561 atacccgcag cacgcgtctc aggcggcagg tgacgcgacg cagccagcgc attgtcgagc
    10621 gccacagcat cggcagcaga caccgcgccg gaggaacgaa cctcctgcag cagctgcgcg
    10681 tcggcgatct ggttcaaacg ggcgttatgg cacgccacca ccccaggagc cacgccgatg
    10741 gtcagcgcac gctgatccag caccggcgca ttcgcgtcgc cgttaccgac agcagccagc
    10801 gcgtcgcccg ggttaaggga tttggtcaga gcctgttcgt cgcgcagacg agcaaccagt
    10861 tgatcgaaca tggacatcac agcacccctc tattcaggtg aggcatacgc cccggacgga
    10921 gcatctcgat ctggccgtag ttagacgaca gaagcgccag ctcaggagcc ggaacgaatg
    10981 cacggttaaa gcggtttttg tcgccgtcat gcacagccag agcacgcagc tcgttaagca
    11041 gagccagctc cagagcacga gcccgggcga tacggcagga gcagatcagc tcgtcaatgg
    11101 caccctcgat ctgatgcacg gcggtgttca tttgcgcatc gagtgccccc acctcccggc
    11161 tcttaatatc caccgcagca tcggcggctt gcacgtcagc gcggatctgg tcgacaacca
    11221 gcgccagcat cgcatcacgg ggctcgggct cgttgccagc cagcaaagca tcacgggcgg
    11281 cggcgatacg cgcctcagca tcagacaggc gagcacgcgc agctttggcg tcgtccttca
    11341 gcttacccag ctcgaagttg agcacaccga tcgcgctggc catctcgtca cgctgcctca
    11401 tcaggtgaag cagggacgca acagcagcat catcgccatc cagcgacggt acagggagat
    11461 cagacagttc agggagggca gagcatgcct cagcgagcac gtctgcaagg tagatccggt
    11521 ttgcgccctc gaaatcgacg ggggcgattt gttcgttttc catagttctc tctctcaatt
    11581 tagccggaac ggctacgcct atcgttgcgt cacgacagat ctggagccag cgcgggagag
    11641 tagaaccggt aggcggcgcg gccttgccgc accccaaagg cggggcgtcg aaagcggcac
    11701 ctttagagta cacgccacct gagcaggaaa acgccgcaaa aaagacgcga tatctgcatc
    11761 ttataagttg agttaatagc tcttattgag catgatggtg acagggaagt ttaagaggac
    11821 acccgcgatg atcaccaaca ccaccgccgt cgcctacatg cgagcgagca cccaggaaca
    11881 ggacgcgaac cgcgcccgcg aggagatcac cctcttcgcc aaagagcacg gcatcaccat
    11941 ccggggcttt tacgccgaga acgtgtccgg cacctatctg gagcgccccg aactcaaccg
    12001 gctactggag gacgccaagc cccacgacat cctcctcgtc gagaagctcg atcgcctgag
    12061 ccgcctcagc gccgaggagt ggagcaccct caaggagcgc atccgcacca agcgcctccg
    12121 catcgtttgc atcgacatac cgaccaccct cgccgtcctg cagggaggcc agccagtagg
    12181 cgcgggcggg tttgacgttc gccgagtggt gctcgacgcc atgaatgaca tgctgctcga
    12241 catcgccgca gcatgggcgc gagacgacta cgagacgcga cggaagcgcc agcaacaagg
    12301 catcgagaaa gcgaagaaac tcggacggtt caaagggaaa cagaaagacg aggagctgcg
    12361 gggcaagatc cgcaccctac tggacacatc cagcatgagc cagaaccaga tcgccaagct
    12421 gatcggctgt tcacggaaca ccgtgagccg ggtcgtcaaa ggcgagtaag aaagagcgcg
    12481 gcggcaaagg tggctccaac cgcctaaccg ccgcaacccc agcccgggct tgccctcgta
    12541 agcgacccgg gcatcatcaa ccagcaaagg taaacgacat gcagatcata caacagacca
    12601 accctgcccg ctac
//
