LOCUS       NZ_CP063500             6846 bp    DNA     circular CON 17-JUN-2023
DEFINITION  Escherichia coli strain EH10-18-47 plasmid pEH10-18-47_1, complete
            sequence.
ACCESSION   NZ_CP063500
VERSION     NZ_CP063500.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN16387496
            Assembly: GCF_016944695.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6846)
  AUTHORS   Moser,A.I., Campos-Madueno,E.I. and Endimiani,A.
  TITLE     Complete genome and plasmid sequences of MDR Escherichia coli from
            local people and environmental sources in Laos
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6846)
  AUTHORS   Moser,A.I., Campos-Madueno,E.I. and Endimiani,A.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-OCT-2020) Institute for Infectious Diseases,
            University of Bern, Friedbuehlstrasse 51, Bern 3001, Switzerland
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Flye v. 2.7.1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 339x
            Sequencing Technology :: Illumina NovaSeq; Oxford Nanopore MiniION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 06/17/2023 05:56:01
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,645
            CDSs (total)                      :: 4,530
            Genes (coding)                    :: 4,300
            CDSs (with protein)               :: 4,300
            Genes (RNA)                       :: 115
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 71 of 230
            Pseudo Genes (incomplete)         :: 155 of 230
            Pseudo Genes (internal stop)      :: 31 of 230
            Pseudo Genes (multiple problems)  :: 27 of 230
            Pseudo Genes (short protein)      :: 4 of 230
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP063500.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..6846
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="EH10-18-47"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pEH10-18-47_1"
                     /geo_loc_name="Laos"
                     /collection_date="2018"
                     /collected_by="Institute for Infectious Diseases Bern"
     gene            complement(join(6485..6846,1..329))
                     /locus_tag="IQS20_RS22300"
                     /old_locus_tag="IQS20_22310"
                     /pseudo=""
     CDS             complement(join(6485..6846,1..329))
                     /locus_tag="IQS20_RS22300"
                     /old_locus_tag="IQS20_22310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /name="IS1 family transposase"
     gene            621..2141
                     /locus_tag="IQS20_RS22305"
                     /old_locus_tag="IQS20_22315"
     CDS             621..2141
                     /locus_tag="IQS20_RS22305"
                     /old_locus_tag="IQS20_22315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000478744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="WP_096282892.1"
                     /translation="MFLISIIQMFSVMIIMLNMKIKDAFRAIILLLLLLLSMVISIEIS
                     SIISIGEYIPTLALANISETNSVGWKIKLLPFLVFLSFMIASLILKRLIKEKKIRNIMV
                     VVFLLSLLIPISPINAFFSSSYEVYSEAKEYNMSPHEISDIKSKYTKEIVTNDSYSHEI
                     INGNGKNILVIFIEGMSSLVISKELTPNLYEFRKKSISFNNYYNHTAATFRGLRGQLTS
                     SYQMTGGYYKDNSGLGQASKVEIEHKLNTYNYITKLPIILEENGYHTYFQASNSIDAPL
                     SLMLSTLKFNHIYGREDYNKKSGELTDKESFDLLFKKLNEAKQPFFYGVYTVGTHLGLD
                     SPDVKFYDGRNEYLNHFHQLDHWFGDFISHFNKTSLSKDTIVIITSDHASYPSEDYKKT
                     FKTDNTFFVDQIPLIIWSNGVEFKDINAHGLNSLSLTPTILDILGIKESHNMFIGESLF
                     EKEKNKNELNCTSAIGDLYVKTCNGKMEIEKRKEKIELIKQLQLFGDR"
                     /name="LTA synthase family protein"
     gene            complement(2155..2995)
                     /locus_tag="IQS20_RS22310"
                     /old_locus_tag="IQS20_22320"
                     /pseudo=""
     CDS             complement(2155..2995)
                     /locus_tag="IQS20_RS22310"
                     /old_locus_tag="IQS20_22320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003030989.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /name="replication initiation protein"
     gene            complement(3087..3323)
                     /locus_tag="IQS20_RS22315"
                     /old_locus_tag="IQS20_22325"
     CDS             complement(3087..3323)
                     /locus_tag="IQS20_RS22315"
                     /old_locus_tag="IQS20_22325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006674368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_078310517.1"
                     /translation="MTEMERQLLSALESLQTSYEQQQQAWQDSYSSLQRMFETTSQALE
                     RNDRVCLNLSNQVNALHEQVERLSSDLSRLTRS"
                     /name="MbeD family mobilization/exclusion protein"
     gene            4625..4942
                     /locus_tag="IQS20_RS22320"
                     /old_locus_tag="IQS20_22330"
     CDS             4625..4942
                     /locus_tag="IQS20_RS22320"
                     /old_locus_tag="IQS20_22330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000718044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000718045.1"
                     /translation="MKKIFILSAALIMAGCATKQYPQAANVTGPESSAMDCKAVALEIA
                     KTRSVQQEIEETGEFDGRTVLGFLGDFGIGNGMAKGEARKKAANRLNQLEALKAAKCAD
                     Q"
                     /name="hypothetical protein"
     gene            complement(5339..5707)
                     /locus_tag="IQS20_RS22325"
                     /old_locus_tag="IQS20_22335"
     CDS             complement(5339..5707)
                     /locus_tag="IQS20_RS22325"
                     /old_locus_tag="IQS20_22335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017880027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_096282894.1"
                     /translation="MANFNPGPRTAIAAVRDSKNDIETWKKNGIRREEIVTVLNERYGL
                     NISLVAFDKALYRLRKSTDKPSLADVDSVSAAETNFSEASGPDNGFFLSGNDFMRAEEE
                     SQRVVESFRDSLGREKGF"
                     /name="hypothetical protein"
     gene            complement(6091..6240)
                     /locus_tag="IQS20_RS22330"
                     /old_locus_tag="IQS20_22340"
     CDS             complement(6091..6240)
                     /locus_tag="IQS20_RS22330"
                     /old_locus_tag="IQS20_22340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017880354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_166509845.1"
                     /translation="MPSEYDEQLDSTDTQLPGEYNLFDLMAQVTEENLHAPVDFGPPVG
                     REVL"
                     /name="hypothetical protein"
     Typing          complement(3669..3754)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="96.552"
                     /coverage="83.0"
                     /evalue="2.14e-32"
     Typing          3721..4228
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="93.922"
                     /coverage="96.0"
                     /evalue="0.0"
ORIGIN
        1 ttcgtccatt tccgcgcaga cgatgacgtc cctgcccggc tgtatgcgcg aggttaccga
       61 ctgcggcctg agttttttaa atggcggaaa atcgtgttga ggccaacgcc aataaagcgg
      121 gcgattgccc ggcatccgac gccattcatg gccatatcaa tgattttctg gtgtgtaccg
      181 ggttgagagg cggtgtaagt gaactgtagc tgccatgttt tacggcagtg agagcagaga
      241 tagcgctgat gtccggcagt acttttaccg ttacgcacaa cgccgtcagt agcggaacag
      301 gagggacagc tgatggaaat ggaagccacc ggagcacctc aaaagcaccg tcatccacta
      361 aatcagtaag ttggaagcat caccgcacta gcagacagtt aattaaaaaa gtataattcc
      421 tctccagatt tgttgaataa tggagaatta tgctgttttc tatgtcttat ttagtcaaat
      481 gcgcttataa atattgtgat aagctcccga gtctaaactt aacttactga ttttaggttg
      541 aggaagaccg acatttttgt agatccacag cataatatct atttattcaa caacgcccca
      601 aaaaaaaaag gtttttataa atgtttttaa tttcaattat tcaaatgttc tcggttatga
      661 ttataatgct taatatgaaa ataaaagatg catttagagc aattattctg cttttacttc
      721 tattactatc catggttata tctattgaaa taagcagcat tatatcaata ggtgagtaca
      781 tacccacact tgcactagca aatatatcag agacaaatag tgttggctgg aaaataaaac
      841 tactaccttt tcttgtattc ttatcattta tgattgcatc attgattttg aaaagattga
      901 taaaagaaaa aaaaataaga aatataatgg ttgttgtttt tttattatca ttgttaatac
      961 caatatctcc aataaatgct tttttttctt catcatatga agtttattcg gaagcaaaag
     1021 aatacaatat gtcaccacat gaaataagtg atataaagtc aaaatacaca aaagaaatag
     1081 taacaaacga cagttactca catgaaataa taaatggaaa cggaaaaaat attttagtaa
     1141 tatttattga aggtatgtct tcattggtaa tatctaagga gctaaccccg aatttatatg
     1201 aatttagaaa aaaatcaata tcatttaaca actattacaa ccataccgca gctacattta
     1261 gaggtttaag aggacagtta acctcatcat atcaaatgac cggtgggtac tataaagata
     1321 attctggcct tggccaagca tctaaagtag agatcgaaca taaactaaat acttacaatt
     1381 acataacaaa attaccaatc atacttgagg aaaatggtta ccatacatat tttcaagcat
     1441 ctaattcaat agatgcccca ttgagtttaa tgctatcaac tttaaagttt aatcatatat
     1501 acggaaggga ggactataac aaaaagtcag gtgaactcac agacaaagag tctttcgatt
     1561 tattatttaa aaagttaaat gaggctaaac aacctttctt ctatggtgtt tatactgtag
     1621 gtacacacct tggcttagat agccctgatg taaagtttta tgatgggaga aatgaatatt
     1681 taaatcactt tcatcaatta gaccattggt ttggtgattt tatatctcat tttaataaga
     1741 caagcttatc gaaagataca attgtcataa tcacaagtga ccatgcaagt tacccatcag
     1801 aagattacaa aaaaacattc aaaactgata atactttttt tgttgatcag attccattaa
     1861 taatatggag taatggtgtt gagttcaaag acattaatgc acacggttta aattcactat
     1921 ctttaacgcc aacaatacta gatattcttg ggatcaagga gtcacacaac atgttcatag
     1981 gtgagtctct atttgaaaaa gaaaaaaata aaaatgaact caactgtaca tctgcaatag
     2041 gtgacctgta tgtgaaaacg tgtaatggga aaatggaaat cgagaaaaga aaagaaaaaa
     2101 tagaattaat aaaacaattg caactttttg gtgatagata attaaaaggc ggttttaccc
     2161 catgttgatt attagtccac gtggggttag tttaaaatga taataaaact tacttttttc
     2221 attcttgagc cgcctgtaga atgtagctcg actaacacct aaagacttcc attctttttt
     2281 taaagttata ggaattaata ttaaaactac ctttaggccg tcctcctgaa ttgcttagat
     2341 ttcctccttt accccctctc tttctttgaa tttctgaacg gtgcgtattt tttatatact
     2401 caataaaacg atcctctgaa aaattattcg ctgtccattt tgagatgctt tttgatattc
     2461 caagaacttc attttcactt aatggtgata cgaattgtaa attgtaggca caggctcgtt
     2521 catagcaggc ttgtaaccac tgctcatact caggccaccc ctgacggatg gcacgatatg
     2581 cccacttgcg ggttttgtcg aacagagtgc agttacggcc taaaccataa tcggcggcaa
     2641 ttttccagtg actgtggttc ggattcttgc aaattagccc cgaatatccc gtgtcagctc
     2701 ccaattttct acgtagcgca ttctctacgg cagctgcgta gcgtaacggt ttcatttttc
     2761 cgtcaggtgc tgtgcgtata gaggtttcca gcgcataaag caggtgcgcg tgtccgtttt
     2821 cggggttggt gatggtcagc gtaggtgcgg gagcgttgcg gtcgctccag tcaatggctg
     2881 cgccgatgcg gtcaacatca aatcccagcc agaacatggc gtgaggctga ttgaactgga
     2941 tgtatttcgc cagaatagcg cgttctttcc ctgcgatgcg cacaccaaag tgcaaatcat
     3001 ccgagaagta cggcttgtgt ggtacatgga ctgacctcga tagcgggttg cttgcttctc
     3061 ctgctgtaac tgccgttcct gctgctttac gagcgtgtta atcggctcag atcgctgctc
     3121 aagcgttcga cctgttcgtg caaggcgttc acctgattgc tcaagttcag acataccctg
     3181 tcgttgcgct ccagtgcctg tgaggtagtc tcgaacatgc gttgcaagct gctgtaactg
     3241 tcctgccacg cctgttgctg ttgttcataa cttgtttgta agctttctaa ggcgctcaga
     3301 agctgccttt ccatctcggt catggttttg cctgtcctca gtgataaaaa cgtgctgtgg
     3361 cttttctgtg ggctctgacg gggttatttc gaggtgtact tcatcacggt gtttccggct
     3421 gtcgatgtat aggtctcggt gccatgcgct ttcttcggca gtaccaccag ataggtgttc
     3481 ttctgcttgt cgtccttgta aatcgctacc cctgcgccgc tctgggcttt caggctctcc
     3541 agttgctggc tgtacggtca caccgttcaa gcagacgctg atgctcagtc tcggtcatgc
     3601 ggattttgat ttctttcgtt cgcttttcca taggctccgc tgagaaaaaa ttcgcaccac
     3661 tgtgagaaag tgcttcatgt ggcaggacaa aaaaggcggc accagtgcgt cagcagaata
     3721 tgtgatacag gatatattcc gcttcctcgc tcactgactc gctacgctcg gtcgttcgac
     3781 tgcggcgagc ggaaatggct tacgaacggg gcggagattt cctggaagat gccaggaaaa
     3841 tacttaacag ggaagcgata gggccgcggc aaagccgttt ttccataggc tccgcccccc
     3901 tgacaagcat cacgaaatct gacgctcaaa tcagtggtgg cgaaacccga caggacttaa
     3961 agataccagg cgtttccccc tggtggctcc ctcgtgcgct ctcctgttcc tgccgttcgg
     4021 tttaccggtg tcattccgct gttatggccg cgtttgtctc attccacgcc tgacactcag
     4081 ttccgggaag gcagttcgct ccaagctgga ctgtatgcac gaaccccccg ttcagtccga
     4141 ctgctgcgcc ttatccggta actatcgtct tgagtccaac ccggaaagac acgacaaagc
     4201 gccactggca gcagccactg gtaacaggat tagcagagcg agttaatgta ggtggtgcta
     4261 tagagttctt gaagtggtgg cctaactacg gctacactgg aaggacagta tttggtatct
     4321 gcgctctgct gaagccagtt accaggttaa gcagttcccc aactgactta accttcgaaa
     4381 aaccacctcc ccaggtggtt ttttcgtttt cagagcaaga gattacgcgt agaaaaaaag
     4441 gaactcaaga agatcctttc tatgctcaac caccgcatgg ctcatagccg cgctggccgt
     4501 tgccggtcag ctccagaatc ctcgaccaat atggaacctt gcccctccca aaattcaact
     4561 aaaaaaccct catttcctgc cgataaaatg ggtatctctt aactatgcct aaataaggat
     4621 aattttgaaa aaaatattca tcctatcggc agcgctgatc atggcaggat gcgcaacaaa
     4681 gcaatatcca caggccgcga acgtcaccgg tccagaatct tccgcaatgg attgcaaagc
     4741 ggtagctctg gaaatcgcca aaacgcgcag tgtacaacaa gaaattgagg aaaccggcga
     4801 atttgacggg cgcaccgtat taggtttcct gggtgatttt gggataggaa atggcatggc
     4861 aaaaggcgaa gcccgcaaaa aagcagctaa ccggctaaat cagctggaag ccctgaaggc
     4921 tgcaaagtgt gcagatcaat aaggtacaaa aaagccctgc ggggcttttt tgtacctgcc
     4981 aatcagctgg atttaccata acatccgttg cccgcaccgc ctgagaggcg ctgagggcgt
     5041 ttctgacggg aaaccggtaa aacaggcaac aaaccacccg aaaacccgcc agaaacgcgc
     5101 aggctctgtt tttacggcat gcatgagtat gcgcgcaagt gcatagcgca gaatttcaca
     5161 ccattcagcc ggggtaagtc ctgtttccga tgttcttctc atccgaaaac ggtgaacagg
     5221 gacgcaattt attgcgtggc cgtgaggcat tgaccaagcc ataaggcacg gtaggtaatt
     5281 gcaggtatcc gacaatttcg agtagaggat ccactatttc actacttaga attcaaaatc
     5341 agaatccttt ctccctgcca aggctatctc tgaatgattc aaccactctt tgagattctt
     5401 cttctgctcg catgaagtca ttcccggaca gaaaaaaacc attgtcaggc ccactggctt
     5461 cagagaaatt cgtttctgca gcagacacag aatccacatc agcaagagac ggtttatctg
     5521 tactttttct caatctgtaa agagccttat caaatgcaac cagtgagata ttcaggccgt
     5581 aacgttcatt cagcacagtc acaatctctt ccctacggat accatttttc ttccaggttt
     5641 ctatatcgtt cttggaatcc ctgacagctg caatggctgt tcttggacca gggttaaaat
     5701 tcgccatata ccgcccataa tgtcttacat ttgtctcata atagtccaaa aataagagta
     5761 aaacgcaaaa aatgtcctac aattgtctta ttgttgtcct ttccggttca tgtatccatg
     5821 aaccgcctga tgtggaacgc tttttatcga tctcatgcgc taccaaagat agattcattc
     5881 tttaaaagtc aggttacatt atcccttaaa tggtcataca ccatacgcaa ccgcgatttt
     5941 catcgcaatt gcatgaatta aaaggtaaaa actgaagtcc ccatttattt atgtaatacg
     6001 caacgtatta caaccacctg taattacatg aaaataaaga aataagatgt aataccggcg
     6061 tattaccacg tacgcggata agcgaaaaag ctataacact tcacggccga caggcggccc
     6121 aaaatccacc ggggcgtgca ggttctcttc agtaacctgc gccattaaat caaaaaggtt
     6181 atattcgccg ggaagctgcg tatcagtcga atccagttgt tcatcatatt cagatggcat
     6241 ggggtgatat ccagtagctg agaggttgtt agcgggtttc ggggcgcagc cctgaaccag
     6301 tcacgtagcg ctagcggagt gtatactgac ttaggcggta gtgtagtatg acagattctg
     6361 tgattacggc aaagtaacag ccctgtctag gagagggctg ttatctgata taggcttgtt
     6421 tttttctcac cgtttttgcg agcgcttttt cctgccaaga ggtaggtagg gtaatgaatc
     6481 caacttactg atagtgtttt atgtttagat aatgcccgat gactttgtca tgcagctcca
     6541 ccgattttga gaacgacagt gacttccgtc ccggccttgc cagatgctgc ctcagattat
     6601 gccgctcaat tcgctgcgta tatcgcttgc tgataacgtg cagcttttcc ttcaggcggg
     6661 attcataaag cggccatccg tccgtcatca ataccaccac gtcaaaggcc gatagcaggc
     6721 tcagaagacg ctccagcgtg aacattgtgc gttcaccaaa aacgtgggcc acgacagtcc
     6781 tgcgtaccca gtcatacgcg taaaacagcc agcgctggcg tgatttagcc ccgacgtagc
     6841 tccact
//
