LOCUS       NZ_CP063497             8908 bp    DNA     circular CON 17-JUN-2023
DEFINITION  Escherichia coli strain EF5-18-41 plasmid pEF5-18-41_5, complete
            sequence.
ACCESSION   NZ_CP063497
VERSION     NZ_CP063497.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN16387482
            Assembly: GCF_016944675.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8908)
  AUTHORS   Moser,A.I., Campos-Madueno,E.I. and Endimiani,A.
  TITLE     Complete genome and plasmid sequences of MDR Escherichia coli from
            local people and environmental sources in Laos
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8908)
  AUTHORS   Moser,A.I., Campos-Madueno,E.I. and Endimiani,A.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-OCT-2020) Institute for Infectious Diseases,
            University of Bern, Friedbuehlstrasse 51, Bern 3001, Switzerland
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Flye v. 2.7.1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 694x
            Sequencing Technology :: Illumina NovaSeq; Oxford Nanopore MiniION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 06/17/2023 06:48:23
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,747
            CDSs (total)                      :: 5,629
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 118
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 272
            CDSs (without protein)            :: 272
            Pseudo Genes (ambiguous residues) :: 0 of 272
            Pseudo Genes (frameshifted)       :: 100 of 272
            Pseudo Genes (incomplete)         :: 199 of 272
            Pseudo Genes (internal stop)      :: 50 of 272
            Pseudo Genes (multiple problems)  :: 66 of 272
            Pseudo Genes (short protein)      :: 4 of 272
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP063497.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..8908
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="EF5-18-41"
                     /isolation_source="chicken stool"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /plasmid="pEF5-18-41_5"
                     /geo_loc_name="Laos"
                     /collection_date="2018"
                     /collected_by="Institute for Infectious Diseases Bern"
     gene            complement(1324..1557)
                     /locus_tag="IQS14_RS28375"
                     /old_locus_tag="IQS14_28380"
     CDS             complement(1324..1557)
                     /locus_tag="IQS14_RS28375"
                     /old_locus_tag="IQS14_28380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_029400396.1"
                     /translation="MTELETHLLNALEQLQQDYMQRLNEWESAFVELQKMFSLTQRDNA
                     MLNERVMQLSQQVQHLSEQTEHLSQLYSESRR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(1564..2082)
                     /locus_tag="IQS14_RS28380"
                     /old_locus_tag="IQS14_28385"
     CDS             complement(1564..2082)
                     /locus_tag="IQS14_RS28380"
                     /old_locus_tag="IQS14_28385"
                     /inference="COORDINATES: protein motif:HMM:NF016710.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_029400393.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTEKVLNNEFRRLGESVSEAVTSNE
                     TKIRDAIALFTASTEESLEKHREGVKEAMRQHRKDVLKLAGNTGMMLLGIVFLLFTASG
                     GTLWYLGGRIQANLEDIRKQEETLQKLNAKTWGVEFVQDGNRKFLVLPYGKSAEVIPFQ
                     GKEWVHLKE"
                     /name="MbeB family mobilization protein"
     gene            complement(<2120..2551)
                     /locus_tag="IQS14_RS28935"
                     /pseudo=""
     CDS             complement(<2120..2551)
                     /locus_tag="IQS14_RS28935"
                     /inference="COORDINATES: protein motif:HMM:NF015397.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(2760..3083)
                     /locus_tag="IQS14_RS28390"
                     /old_locus_tag="IQS14_28395"
     CDS             complement(2760..3083)
                     /locus_tag="IQS14_RS28390"
                     /old_locus_tag="IQS14_28395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_000955999.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGKLPTL
                     APPLLRQLAAIGNNLNQTARKVNSGQWSSGDRVQVVAALMAIGDELRRLRLAVREQGAR
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            3148..3339
                     /locus_tag="IQS14_RS28395"
                     /old_locus_tag="IQS14_28400"
     CDS             3148..3339
                     /locus_tag="IQS14_RS28395"
                     /old_locus_tag="IQS14_28400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165974.1"
                     /translation="MTKQEKTALNMARFIRGQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            4009..4167
                     /locus_tag="IQS14_RS28400"
                     /old_locus_tag="IQS14_28405"
     CDS             4009..4167
                     /locus_tag="IQS14_RS28400"
                     /old_locus_tag="IQS14_28405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001354639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001354639.1"
                     /translation="MHEPPVQPDRCALSGNYRLESNPVRHDKAPLAAAVGNLVSRAMMA
                     QMVQQSS"
                     /name="hypothetical protein"
     gene            4549..4890
                     /locus_tag="IQS14_RS28410"
                     /old_locus_tag="IQS14_28415"
     CDS             4549..4890
                     /locus_tag="IQS14_RS28410"
                     /old_locus_tag="IQS14_28415"
                     /inference="COORDINATES: protein motif:HMM:NF015489.2"
                     /GO_function="GO:0015643 - toxic substance binding
                     [Evidence IEA]"
                     /GO_process="GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E1 family microcin immunity protein"
                     /protein_id="WP_072648848.1"
                     /translation="MSLRYYIKNILFGLYCTLIYIYLITKNNEGYYFLASDKMLYAIVI
                     STILCPYSKYAIEHIFFKFIKKDFFRKRKNLNNAPMAKLNLFMLYNLLCLVLAIPFGLL
                     GLFISIKNN"
                     /name="colicin E1 family microcin immunity protein"
     gene            complement(4887..6451)
                     /locus_tag="IQS14_RS28415"
                     /old_locus_tag="IQS14_28420"
                     /pseudo=""
     CDS             complement(4887..6451)
                     /locus_tag="IQS14_RS28415"
                     /old_locus_tag="IQS14_28420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447006.1"
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA];
                     GO:0050829 - defense response to Gram-negative bacterium
                     [Evidence IEA]"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like pore-forming protein"
                     /name="colicin-like pore-forming protein"
     gene            6729..7148
                     /locus_tag="IQS14_RS28420"
                     /old_locus_tag="IQS14_28425"
     CDS             6729..7148
                     /locus_tag="IQS14_RS28420"
                     /old_locus_tag="IQS14_28425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001749639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000312320.1"
                     /translation="MAWHTLQNALKMTGRSRSQFYRDMRAGLVSYRTGQDGRREFETSE
                     LIRAYGELKQDETPERHSAGQAENPHDQQTERILRELNELRQCLTLMLEDKQAQATERR
                     RQEAEREQLHKEIEQLRQALEQEKKRGFWARLFSR"
                     /name="hypothetical protein"
     gene            7232..7651
                     /locus_tag="IQS14_RS28425"
                     /old_locus_tag="IQS14_28430"
     CDS             7232..7651
                     /locus_tag="IQS14_RS28425"
                     /old_locus_tag="IQS14_28430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="WP_001254598.1"
                     /translation="MRRSGGTLYLIALLTAATVLTACTPKGSVEQHTRHYVYASDDGFD
                     PNFSTQKADTTRMMVPFFRQFWDMGAKDKATGKSRSDVQQRIQQFHSQEFLNSLRGTTQ
                     FAGTDYRSKDLTPKKSRLLAETISAVYLDGYEGRQ"
                     /name="Exc2 family lipoprotein"
     gene            8177..>8353
                     /locus_tag="IQS14_RS28430"
                     /old_locus_tag="IQS14_28435"
                     /pseudo=""
     CDS             8177..>8353
                     /locus_tag="IQS14_RS28430"
                     /old_locus_tag="IQS14_28435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611469.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0004803 - transposase activity [Evidence IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /name="transposase"
     Typing          complement(3425..3512)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="97.753"
                     /coverage="100.0"
                     /evalue="1.48e-35"
     Typing          3535..4081
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="93.841"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          complement(1221..2770)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="94.656"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 caaggaaatc ataatcagcg accaaaccat ttatcaacag aaaaccaaca tattgatgtt
       61 atttttgtga caaatgttac aaattcgaaa ccactgtatt tttactgcaa caaaatctgg
      121 ttgatctaca agttacactg gggtaaaaag ttgtccggaa tgtttctgcg tagtaaagcc
      181 tctagccgga ttgcctttgc aaaaacgcag gaacgttccc cccttcatca gtactcctga
      241 cgctgtaagg agctgactga tgcagacagg gctgatgata tctaataaaa gctatctcat
      301 ttttgcgcgt acataaaata aatcaggcac ttccctacac tccccctccg gaattgaggg
      361 ggttttcttt acaatgtaaa taaattgctt gcgtaaagtt caaaaaaact gcaaattttt
      421 actgtagtgt cgagcttcat gtagctcacc agggcatgga ctaaaaagaa gtaaacccat
      481 cttttacccc cccttatacg ggggttcttt atagcacaaa gaacacttat aatttatcat
      541 ctcacagcta taaatataaa agaataatat tctaaactac agttatttat gcagaacata
      601 acatcaaact cacaataaat ataaaacaat tattttataa ctgttcactt attatattca
      661 gacatatcag aaacacgact caattctttt ttatttaaca aaatccataa aaaacttatg
      721 gttgaattca catttaaact gtgaaggata tcatccggag aaatttttca atccgacact
      781 ccatagaatg gacctttaag actttatcct catcatgtat gaggattttt tttctggcaa
      841 acagtatctt tcagtaatcc ttgctcagtt ccgattctga cgtatccccg tgtggatctg
      901 aaaaggagat aattacgctc tgtggccaac aggaataact caagcagctc ctctctgata
      961 ccatcagaag aagcattaaa cgcattctgg cgtcgattac agaagaagcg gaacgcgttc
     1021 caggctcctg ttgctataca cactacccac actaacccca tggtttttga gtgccagaaa
     1081 cgcgtccgta tgcgttttaa cgcgttcctg tgttttcagc aaggcctgac tgaagctagt
     1141 tcctagtgcc ctgaaccaac cattatccaa aaaaatgcac cggaaacgcg ctgacaacac
     1201 tttagcctgt ctttggttac catcccagcg acgggccatc cagtgtccgt tcctgtattt
     1261 ccagatggcg ctcacgctct aggaatagct catgttcacg ttcctgtatc agtagttcgt
     1321 cccttatctc ctgctctcgc tgtataactg gctcaagtgt tctgtctgct cgctcaagtg
     1381 ctgcacctgc tgactcaact gcatgacccg ctcgttcagc atcgcgttgt cccgttgcgt
     1441 aagcgaaaac atcttctgca attccacgaa ggcgctctcc cattcgttca gccgctgcat
     1501 atagtcctgt tgcagctgct ctaaggcgtt cagcaaatgt gtttccagct ctgtcactct
     1561 gtgtcactcc ttcagatgta cccactcttt cccctgaaag ggaatcacct ctgctgattt
     1621 cccgtacgga aggaccagaa atttcctgtt cccgtcctgc acgaactcca cgccccatgt
     1681 cttcgcgttc agtttctgca atgtctcttc ctgcttcctg atatcttcca ggttcgcctg
     1741 tatcctcccg ccaagatacc agagcgtccc gccactcgcg gtaaacagga gaaagactat
     1801 ccccagtaac atcatgcccg tattccctgc cagctttaac acgtccttcc tgtgctgcct
     1861 catcgcctct ttcacccctt cccggtgttt ctccagcgat tcctctgtcg aggctgtgaa
     1921 cagggctata gcgtctctga ttttcgtctc gtttgatgtc acagcctcgc ttacagattc
     1981 gccgagcctc ctgaactcgt tgttcagcac tttctctgta gattcggctc tctctttcag
     2041 ctttttctcg aactccgcgc ccgtctgtaa aagattgctc ataaaacgct cctttcagcc
     2101 tgatattctt cccgccgttc gggtctgcaa tgctgatgct gcttcgcgtc accctgacca
     2161 cttcaagccc cgcctctgtg agcgcctgaa tcacatcctg acggcctttt atctccccta
     2221 catgataaag agcgtctatc ccgcgtgtga cgctctcagc aagcgcctgt ttcgtttccg
     2281 gcaggttatc agagagagtc agtatccggc ggttctccgg ggcgttgggg tcatgcagcc
     2341 cgtaatggtg attcaccagc gtctgccagg catcaattct cggcctgtct gcccggtcgt
     2401 agtacggctg gagacgtttt ccggtctgta gctccatgtt cggaatgaca aaattcagct
     2461 caagccgtcc cttgtcctgg tgctccaccc acaggatgct gtactgattc ttttcgagac
     2521 ctggcatcag tacgcgctca aagctcgcca tcactttttc acgtcctccc ggcggcagct
     2581 ccttctccgc gaacgacaga acgcctgacg tgtatttctt cgcaaatggc gtggcatcga
     2641 tgagttcccg gacttcttcc ggattaccct gaagcaccgt tgcgccttcg cggttacgct
     2701 ccctccccag caggtaatca accggaccac tgccaccacc ttttcccctg gcatgaaatt
     2761 taactatcat cccgcgcccc ctgttccctg acggccagac gcagccggcg cagctcatcc
     2821 ccgatggcca tcagtgcggc caccacctga acccgatcac cggaagacca ctgcccgctg
     2881 ttcaccttcc tcgcggtctg attcaggtta ttcccgatgg cggccagctg acgcagtaac
     2941 ggcggtgcca gtgtcggcag cttcccggaa cgggcaaccg gctcccccag gcagacccgc
     3001 cgcatccata ccgccagttg tttaccctca cagcgttcca gtaaccgggc atgttcatca
     3061 tcagtaaccc gtatcgtgag catcctctcg cgtttcatcg gtatcattac ccccatgaac
     3121 agaaatcccc cttacacgga ggcatcagtg actaaacagg aaaaaaccgc ccttaacatg
     3181 gcccgcttca tcagaggcca gacattaacg ctgctggaga aactcaacga actggacgcg
     3241 gatgaacagg cagatatctg tgaatcgctt cacgaccacg ccgatgagct ttaccgcagc
     3301 tgcctcgcac gtttcgggga tgacggtgaa aacctctgac acatgcagct cccggagacg
     3361 gtcacagctt gtctgtgagc ggatgccggg agccgacaag cccgtcaggg cgcgtcagca
     3421 ggttttagcg ggtgtcgggg cgcagccctg acccagtcac gtagcgatag cggagtgtat
     3481 actggcttag tcatgcggca tcggtgcgga ttgtatgaaa agtgcaccat gtacggtgtg
     3541 aaatgccgca cagatgcgta aggagaacat gcagatgccg atgctcttcc gcttcctcgc
     3601 tcactgactc gctgcgctcg gtcgttcggc tgtggcgagc ggtgtctgct cactcaaaag
     3661 cggtgatact gttatccaca caatcagggg ataacgccgg aaagaacatg tgagcaaaaa
     3721 acgaagaccc cagaaaaggc cgcgccggag gcgctttttc cataggctcc gcccccctga
     3781 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gacttaaaga
     3841 taccaggcgt ttccccccgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
     3901 accggatacc tctccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc
     3961 tgttggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
     4021 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
     4081 agacacgaca aagcgccact ggcagcagcc gttggtaact tggttagcag agcgatgatg
     4141 gcgcaaatgg tgcaacagag ttcttgaagt agtggcccga ctacggctac actggaagga
     4201 cagtatttgg tatctgcgct ctgctgaagc cagttacctc ggttaagcag ttccccaact
     4261 gacttaacct tcgatcaaac cgcctcccca ggcggttttt tcgtttacag gcagcagatt
     4321 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctactga acagagatcc
     4381 ccgtcagttt agaagaagat gatggagcca cagtccctcc ctgaacatca cgtatatagt
     4441 tagcctgaca tccaacaagg aggtttatag ctaatattcc cacaaaaaat cttttcctca
     4501 taactcgatc cttataaaat gaaaagaata tataacgagg tttaatttat gagtttaaga
     4561 tactacataa aaaacatttt gtttggccta tattgcacac ttatatatat ataccttata
     4621 acaaaaaaca acgaagggta ttatttccta gcgtcagata agatgctata cgcaatcgtg
     4681 ataagcacta ttctatgccc atattcaaaa tatgctattg aacacatatt ttttaagttc
     4741 ataaagaaag attttttcag aaaaagaaaa aatctaaata atgcccccat ggcaaaatta
     4801 aacctattta tgctatacaa tctactttgt ttggtcctag caatcccatt tggattgcta
     4861 ggactgttta tatcaataaa gaataattaa atacccaaca catcatttat agtattaagt
     4921 ttattcttgt caataaaggc acatagaatg cctgtaacaa tagcaatccc ccagatacct
     4981 aatgtagttc cagcaagcac actaaaaagt aaaacaacaa tataactaac tccagcatct
     5041 acagttttct tctctaatgt aagaaatagt ggcttccagt cacctgtatc cttaattttt
     5101 aggatatcag ataccacatc atatccaaaa gaaacatgtc ccgtaatctt caagtactta
     5161 gcaaactgat ctaaatgctt agcccagtct tcatacttaa ccgattccag tgcattgaaa
     5221 atcgcatcac ggtctgcttt gctgaatttc ttatttaaaa catccttgta tttttcaaaa
     5281 gcagctagag cttcattcac attgctgatt ttcttacctt tagacttatc agcaagttcc
     5341 tgtgccattt ttgaatattt tcaccatatt tttcagatag cgtttgataa aagctaactg
     5401 ttgcatccac agcatcctta atctgcgagt tcaggagatt agtctgcgct gttttcaaat
     5461 tttcttcaga ttcatgaaca cgtaaaacag ctgtattacg attattatta gcctgagaca
     5521 tagccccctg aattccattt atctcagcat taagttggtt aatacgcgtt tctgatgctg
     5581 ttacctgttt ttgtttttcc tccataatct taccggctcc cgtccgtcga ctggtggcat
     5641 agaaaaaagg acgactctga agcggatcat tagctcttgg tgacaatttt ttgaccagtt
     5701 catcaagttc cttatattta gcagatgcct gagccagctc atttcgtttt ccggacagcg
     5761 tattcatttc tgcatcacgg gcatgaatac tggagcttaa acgggtattg agagtcttaa
     5821 tctcttcatc cactttcgcc agctcagatt gtgcagcagc aagatttttt tgtgccacct
     5881 ccactgcccg ggcctcttca ttcaatgctg ccagccgttt ctcttcagct tcagccagtt
     5941 tcaactggcg ttctgtttca gcctgctcct tctctatctc tttacgtcgt tgttctgcct
     6001 cctgaaaagc cttttccgca gcttccgctt ctttacgggc tttttcttct gcttttgcaa
     6061 ggcgcaaccg ctctgcttct gcctgcatcg ctgcattatt ggcatgagcc aggtcaataa
     6121 cctccggatg agtggaatta tgacgaagcg cctcattcac aatatccttc agatgttgag
     6181 tcagcgcatc ccggtttgct tttgctttag cctgtgcttc tgcggcagct tttgcccggg
     6241 cagcctgttc tgcctgcgtt ttcttcaatt gagcagtaga ccatttagct gtggcatgaa
     6301 tggctgcaga actttcactt ttgctacctc cagttccacc accgccgcca gagccactcc
     6361 cgtctggatt accattcaaa agagtaatga ttacctgccc cttatcatca taaggaacac
     6421 catctttata gtacgctaca gctgtttcca ttataaaatc ctctttgaca ttaaaaacaa
     6481 atagttaaaa gcaatatact gtatatataa ccactggttt tatatacagc ttaaaattac
     6541 gccgctgcat tttccctgtc aaccctgtgg attttcattt ttgtgaaaac gatcaaaaaa
     6601 acagccaccc acaccacgtg ctattcccac ccgatgccac aaaaaccagc acaaacatta
     6661 ccgttctccg gccccagtat gttttactga aaccatgata tgagacatta atgggacact
     6721 gtcacttcat ggcatggcac acactccaga acgcactgaa aatgacaggc agatcgcgtt
     6781 cacagtttta ccgtgatatg cgcgcaggcc ttgtcagtta tcgtacaggc caggacggac
     6841 ggcgggagtt tgaaaccagt gaactgatcc gggcatacgg cgaattaaag caggatgaga
     6901 caccagaaag gcacagtgcg ggacaggcag aaaatccaca tgatcagcag acagaacgca
     6961 ttctccggga gctgaatgag ctgagacaat gcctgacact gatgcttgag gacaaacagg
     7021 cacaggctac ggaacgccgg cgccaggaag ccgaacggga acagctacat aaagagatag
     7081 agcagctcag gcaggcactg gagcaggaaa aaaaacgggg attctgggcc agactgttca
     7141 gccggtgagc accgactgca agtgatgata agatagtcag cggttaaata taagtcactg
     7201 agtacaaaga tttaaaggta aggagacact catgagacgt tccggcggca cactgtatct
     7261 tatcgccctg ctgacagcgg caacggtcct gacagcctgc acgccaaagg gcagtgtgga
     7321 acaacatacc cggcattacg tttatgcatc agacgatggt tttgatccta acttttccac
     7381 ccagaaggcc gacacaacac gaatgatggt gccttttttc cggcagttct gggatatggg
     7441 agcaaaggat aaagcgacag gaaaatcacg gagtgatgtg cagcaacgca ttcagcagtt
     7501 tcacagccag gaatttttaa actcactccg gggcacaact caatttgcgg gtactgatta
     7561 ccgcagcaaa gaccttaccc cgaaaaaatc caggctgctg gctgagacga tttctgcggt
     7621 ttatctcgat ggctacgagg gaagacagta agtggattta ccataatccc ttaattgtac
     7681 gcaccgctga aatgcattca gcgcaatcac ggcagcagac aggtaaaaat cgcaacaacc
     7741 cccccgaaaa actaccgcga tcgcgcctga taaattttaa ccttatgcat acctatgcat
     7801 ccaggtgaat cacaagcgaa tttccttact gagacgactg aaacggtttt ttttcacaat
     7861 tttcagctgt tttcgattcc acaaaataga agttacagga tagggattaa tccccttcgc
     7921 cgccaagggg agcaggatga gtcaggggaa tgtctattca cctgcgctgt acaggctgct
     7981 aggtaaaagt attcgctgcg gcacgatact cttttgccct catagccttt gcgcttctta
     8041 cttttcgttc ttccagaatc attcttaaga cattttcccg cacttttctc taacttcccg
     8101 tggcaattta tctctgccgg actgaaccgc cccggaaatc ctggagacta aacttcctga
     8161 gaaagatgta aacaggatga ctaaaaatac tcgtttttcc cccgaagtcc gtcaacgggc
     8221 agttcgtatg gttctggaaa gccagggcga atatgactca caatgggcgg caatttgttc
     8281 cattgctcca aagattggct gtacgccaga gaccctgcgt gtctgggttc gccagcatga
     8341 gcgggatacc gggtattacc tttcgaagaa atttttgaca tcctctacgc cctgaaggac
     8401 cgggttttac ggcgtaccag ataaaaaaac ccctctactg aagaggggta gtgtaaggaa
     8461 cttgctgatt ttcttgatat tattaaccat caataactga gcatacaaaa aataacaaaa
     8521 ctctgacaca gatacaacca tccctctttg tataagaccg atgtgcatct caaaaaagaa
     8581 gcgtgtttct ggaacacaga acaacaatgc acattgtagc caacaaattc acaccagaaa
     8641 cacgctctgt tttttaattt acacaagaat atatgtctgg aaaatcagga agaagaaatt
     8701 aatgggcatt ctgtatacga agggagagcg cgtttccggt gccccatatt agatattatc
     8761 cctgcaacaa acaccagaga cgcgctcata attcttctag cgtaaatgat cctttcaggc
     8821 aagaaaaaag ccgtgattct gtatcacggc cttaatttta tcgaaaacac ggattttagt
     8881 atgcacatga ctgtactcct gtggagta
//
