LOCUS       NZ_CP063491             9580 bp    DNA     circular CON 17-JUN-2023
DEFINITION  Escherichia coli strain EF7-18-51 plasmid pEF7-18-51_4, complete
            sequence.
ACCESSION   NZ_CP063491
VERSION     NZ_CP063491.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN16387483
            Assembly: GCF_016944655.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9580)
  AUTHORS   Moser,A.I., Campos-Madueno,E.I. and Endimiani,A.
  TITLE     Complete genome and plasmid sequences of MDR Escherichia coli from
            local people and environmental sources in Laos
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9580)
  AUTHORS   Moser,A.I., Campos-Madueno,E.I. and Endimiani,A.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-OCT-2020) Institute for Infectious Diseases,
            University of Bern, Friedbuehlstrasse 51, Bern 3001, Switzerland
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Flye v. 2.7.1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 390x
            Sequencing Technology :: Illumina NovaSeq; Oxford Nanopore MiniION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 06/17/2023 06:45:31
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,681
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,324
            CDSs (with protein)               :: 4,324
            Genes (RNA)                       :: 119
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 61 of 238
            Pseudo Genes (incomplete)         :: 162 of 238
            Pseudo Genes (internal stop)      :: 57 of 238
            Pseudo Genes (multiple problems)  :: 40 of 238
            Pseudo Genes (short protein)      :: 3 of 238
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP063491.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..9580
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="EF7-18-51"
                     /isolation_source="chicken stool"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /plasmid="pEF7-18-51_4"
                     /geo_loc_name="Laos"
                     /collection_date="2018"
                     /collected_by="Institute for Infectious Diseases Bern"
     gene            complement(join(9260..9580,1..147))
                     /gene="traD"
                     /locus_tag="IQS15_RS23105"
                     /old_locus_tag="IQS15_23125"
     CDS             complement(join(9260..9580,1..147))
                     /gene="traD"
                     /locus_tag="IQS15_RS23105"
                     /old_locus_tag="IQS15_23125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016517641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraD"
                     /protein_id="WP_016946647.1"
                     /translation="MNKSEKQEQWAAERVQYIRGLKSPNEQQKLMLILTDKADKTAQDI
                     KTLSLLMKAEQAAEKAQEARAKVMNLIQAEKRAEARAARKARDHALYQSAGLLILAGLV
                     DSKTGKPVDDTAALLGALASLNDLSRDNPKWSDWKIRGQELLNSKKSDSTT"
                     /name="traD"
     gene            311..1426
                     /gene="mobA"
                     /locus_tag="IQS15_RS23110"
                     /old_locus_tag="IQS15_23130"
     CDS             311..1426
                     /gene="mobA"
                     /locus_tag="IQS15_RS23110"
                     /old_locus_tag="IQS15_23130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012443156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein MobA"
                     /protein_id="WP_016946648.1"
                     /translation="MASYHLSVKTGGKGSASPHADYIAREGKYAREKDSDLEHKESGNM
                     PAWAAHKPSEFWKAADTSERANGCTYREIEIALPRELKPEQRLELVRDFVQQEIGDRHA
                     YQFAIHNPKAAIAGGEQPHAHIMFSERINDGIHRDPEQYFKRANTKEPERGGAKKARFG
                     ETPTERKEYLVAQRERWADLQNKHLERYQHADRVDSRSLKAQGIDREPERHLGAGQVQR
                     FDTEQLQAILERREAERQVQQSRDERDSVIDVTTSLREALSERDTLTLKQELKSEPEQE
                     SHSGRTFDFEKEPDKLNALVSDAMKDIQEEIDLQSLVNDAMAEFQGIHQEMERQRERER
                     LAEKQRQQEKERQRLAEQIRQKPDKGWSFSR"
                     /name="mobA"
     gene            1439..2023
                     /locus_tag="IQS15_RS23115"
                     /old_locus_tag="IQS15_23135"
     CDS             1439..2023
                     /locus_tag="IQS15_RS23115"
                     /old_locus_tag="IQS15_23135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein MobX"
                     /protein_id="WP_010891053.1"
                     /translation="MSDEIKQIAALIGHHQALEKRVTSLTEQFQAASSQLQQQSETLSR
                     VIRELDSASGNMTDTVRKSVSSALTQVEKELKQAGLAQQKPATEALNQAADTAKAMIHE
                     MRREMSRYTWKSAIYLVLTIFFVLASCVTAFTWFMNDGYSQIAEMQRMEAVWQKKAPLA
                     DISRCDGKPCVKVDTRTTYGDKENTWMIIKK"
                     /name="mobilization protein MobX"
     gene            2136..7058
                     /locus_tag="IQS15_RS23120"
                     /old_locus_tag="IQS15_23140"
     CDS             2136..7058
                     /locus_tag="IQS15_RS23120"
                     /old_locus_tag="IQS15_23140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005204747.1"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA];
                     GO:0003677 - DNA binding [Evidence IEA]; GO:0016787 -
                     hydrolase activity [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type ISP restriction/modification enzyme"
                     /protein_id="WP_152952528.1"
                     /translation="MNALSALLTKIEQASPTQRDKGTTFENLCVQYFLHEPKYAELYSD
                     VLSYGGWVSQYGETVGITKKKDDGIDLVAVTKTGEFHAIQCKNYNQTKIAKKDIDSFLA
                     ASDKTYFTLRYIVASTDNWTEEAKNMLRDKAVPVTALSLTDLEQSALDWSQFDFDPAYK
                     PVMKAKKQLRPHQTPALEAVKRGLTTADRGKLIMACGTGKTFTSLRIAEAVAGRGKTVL
                     FLVPSLALLSQTLDEWTQDTLIDLRCFAVCSDSDVGKKNHDDNVVVGISDLKYPATTNA
                     NSLVKAFNQPDIFGSDKPPYMNVVFSTYHSVEVIHQAQKLGFPTFDFIICDEAHRTTGA
                     TFEGDDESAFVRIHDNAYIAGQKRLYMTATPRIFGDDAKETEGVTLCSMDDKSLYGDDL
                     YVITFSKAVQLGILCDYKVIVLAVEEKHVSRRIQSLLKDENNQLKVDDAAKIVGCWKAL
                     AKQGLAGSDGVHPDAMKRAVAFCQVIEKDFRGKTHKVSSKLITEMFGAVVEAYQASEIE
                     LLRETAPDKAIDPSLKLQCQVKHVDGSMNATEKKSKIEWLKADTDENVCRILSNVRCLS
                     EGVDVPSLDAVLFLTPRSSQVDVVQSVGRVMRRAEGKELGYVILPVVIPAGEEPENALN
                     NNQAYRVVWQVLNALRAHDDRFDAMINKLEFNGKDTSRMEVIAVADKVVKKTKRQTKKS
                     ELAGKARKSSGIGSAVSPVPEQFDIEFSVGEIERALYAKIVKKCGNRHHWEDWANDIAK
                     IARTHIDRIHGILENPGNQVEISAFRAFADELRDDLNNSVSDDEIIEMLAQHLITKPVF
                     DALFADYNFTDHNPMSMAMQNVLNTLQEHHLEKEASTLNSFYESVKMRADGITTAEGKQ
                     QIIVQLYDKFFRNAFPRMTERLGIVYTPVEVVDFIIHSVNDVLKKEFGQTLADKGVHIL
                     DPFTGTGTFITRLLQSGLIPSDKLAYKFKNEIHANEIVLLAYYIAAINIEAVYHGITEE
                     SEYTPFEGICLTDTFEMYEKDDLVSEVLVDNSERRKRQKALDIRVIIGNPPYSSGQESA
                     NDNNQNVKYPMLDKRIAETYAASTTATNKNALYDSYIRAIRWASDRIGEQGVIGFVTNG
                     GYIDSNSADGLRKCLAEEFSSLYFFHLRGNQRTSGEKSRQEGGKIFGSGSRAPIVIAIL
                     VKNPAATQSGQIYFHDIGDYLTREEKLEKISDFGSIDGIEKTHGWQKITPDAYNDWLNQ
                     RDDSFYDFIQIGNKKDKENSSLFDVYSTGLQPRGDAWFYNYSKQKLAMNIKKSINFFNS
                     EVDRYKKEYNSGSLKDFVRIDPFNFAWYGESYKEISRDRYYDIADGVVRVSLYRPYSKS
                     LVYFNENMMHRVSKIPEIFPEKNSNNLIIIITGRGSTKGFSSLISNEIVDLECISKGQC
                     FPLYVYDNKKPSILFGNQDESVYLDGEYERKYAISDTGMIYFKKHYPEEKINQEDVFYY
                     IYGLLHSEDYRSRYADNLSKELPYIPCVKKIEDFWSFSKAGRELAELHLNYETVEPYKA
                     DIDTGSLSYSQLGKDDFYVEKMKFAKKGEKDTVIYNNKIRIKNIPIEAYDYIVNGKPAL
                     EWVMERQGVSTHKDSGIVNDANDWAIETMNNPRYPLELFLRVITVSLETQKIVNSLPKL
                     DI"
                     /name="type ISP restriction/modification enzyme"
     gene            complement(7294..7707)
                     /locus_tag="IQS15_RS23125"
                     /old_locus_tag="IQS15_23145"
     CDS             complement(7294..7707)
                     /locus_tag="IQS15_RS23125"
                     /old_locus_tag="IQS15_23145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001229901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_040090700.1"
                     /translation="MALVSISEASRLTNKSRTTVHRYISKGKLSICTDENGVKKIDTSE
                     LLRVFGAFKAVHHEQLDNVTDEQHVTPNRTGETSKTKQLEHEIEHLRQLVTAQQSHIDS
                     LKQAMLLIESKLPTTREPVTPPVGKKSWQFWKK"
                     /name="hypothetical protein"
     gene            complement(7715..8665)
                     /locus_tag="IQS15_RS23130"
                     /old_locus_tag="IQS15_23150"
     CDS             complement(7715..8665)
                     /locus_tag="IQS15_RS23130"
                     /old_locus_tag="IQS15_23150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016946646.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_016946646.1"
                     /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRKE
                     RTVSFTYNQYAQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFEIFQWTNYAKFS
                     SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI
                     EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV
                     ELDRQMDLVTELENNQIKMNGDKIPTTITSDSHLHNGLRKTLHYALTAKIQLTSFEAKF
                     LSDMQSKYDLNGSFSWLTQKQRTTLENILAKYGRI"
                     /name="replication initiation protein"
     Typing          complement(7716..7891)
                     /name="ColE10"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="99.435"
                     /coverage="100.0"
                     /evalue="9.330000000000001e-86"
     Typing          312..1423
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="94.07"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 ggacagcgtt ttgatatcct gtgctgtttt atctgcttta tccgtcagta tcagcataag
       61 tttctgttgc tcattcggcg actttaagcc gcgaatatac tgcactcttt ctgctgccca
      121 ttgttcctgt ttttcgcttt tattcatgct gtttacctcc tgaatgctaa cggaatgatt
      181 atgctcagtg atggcagagg aggtcaaatc ccactacaat ccctgataac tcttttattt
      241 atcagggcgc acttagacgt ttcttcactt cgttcagaaa cggtgcgcgc cttcagcggg
      301 ataaaacacg atggcatcct atcatctcag cgtaaaaacc ggcggaaaag gcagtgcctc
      361 tcctcatgcc gattatattg cgcgtgaggg aaagtatgcc cgtgaaaaag actccgacct
      421 tgagcacaag gagtccggca atatgccggc gtgggcagcg cataaaccgt ccgagttctg
      481 gaaagccgct gacacgtctg aacgtgccaa cggctgtacc taccgtgaaa tcgagattgc
      541 cctgccgcgt gagttaaaac ccgaacaacg tcttgagctt gtccgcgatt ttgtccaaca
      601 ggaaattggc gaccgccatg cctatcagtt cgctatccat aatccgaaag cagcgatagc
      661 cggtggcgag cagccccacg cccatatcat gttctctgaa cgcattaatg acggtattca
      721 ccgtgaccct gagcagtatt ttaaacgggc taacaccaaa gagccggagc gcggtggcgc
      781 aaaaaaggca cgtttcgggg aaacaccgac cgaacgcaaa gaataccttg ttgcccagag
      841 ggaacgctgg gctgacctcc agaataagca ccttgaacgg tatcagcacg ctgaccgggt
      901 tgattcccgt tccctgaagg cgcagggcat tgaccgtgag cctgagcggc atctgggcgc
      961 gggtcaggtt cagcgattcg atactgagca gctacaggcc attcttgagc gcagggaggc
     1021 tgaaaggcag gtgcagcagt cccgtgatga acgtgacagc gtaattgacg tgactacctc
     1081 gttacgggaa gccctcagcg agagggatac tctgacgcta aaacaggagc tgaagtcaga
     1141 gcctgagcag gaatcccatt ccggcagaac gtttgatttt gaaaaagagc ctgacaagct
     1201 gaacgcactg gtcagtgatg ccatgaagga tatacaggaa gaaatcgatc ttcagtccct
     1261 tgttaacgat gcgatggcgg agttccaggg gatacatcag gagatggaaa ggcagagaga
     1321 gcgcgaacgt ctggcggaaa aacagcgtca gcaggagaaa gaacgacagc gactcgccga
     1381 acagatacga cagaagccgg ataaaggctg gtcattctca cgataggcag gattttgtat
     1441 gagcgacgaa attaaacaaa ttgccgccct tatcgggcat catcaggcgc tggaaaagcg
     1501 tgttacttct ctgacagaac agtttcaggc cgcctcatca caattacagc agcaaagtga
     1561 aacgctgagc agggtcattc gtgagctgga cagcgcgtcc ggtaatatga ctgacaccgt
     1621 caggaaatcg gtgagttccg cgctcactca ggttgagaaa gaactgaaac aagccggatt
     1681 agcacagcag aaaccggcga cagaagcgct gaatcaggct gccgatacgg cgaaagcgat
     1741 gatccatgaa atgcgtcgtg agatgtcacg ctatacgtgg aaatccgcga tttatcttgt
     1801 actgacgata ttcttcgtcc tggcttcctg cgtgacggca tttacgtggt ttatgaatga
     1861 cgggtatagc cagattgctg aaatgcagag gatggaagcg gtctggcaaa agaaagcccc
     1921 gctggctgac atatcgcgct gtgacggaaa gccctgcgtt aaggtggaca ctcgcactac
     1981 gtatggtgat aaagagaata cctggatgat tattaagaaa taacatcata gagcacagaa
     2041 cgctgtgcgt cgatctccgg cggagaaagc attaattaaa cgtgtcgggg ggatctgata
     2101 accccagcaa ataagggaca ttaaggatat caacgatgaa tgcactttct gcattgctga
     2161 caaaaattga acaggcttca ccaacacagc gggacaaagg tacaaccttt gaaaaccttt
     2221 gtgtgcagta tttcctccat gaacctaaat atgccgagct gtacagtgac gtgctgagct
     2281 atggcggatg ggtttctcag tacggtgaaa ccgttgggat cacgaagaaa aaagatgatg
     2341 gcattgattt ggtcgccgta accaaaacgg gggagtttca tgctatccag tgcaaaaact
     2401 acaatcagac taaaatcgcc aaaaaggata ttgatagttt tctggctgcc tccgataaaa
     2461 cgtattttac gctccgctat atcgttgcct ctacggataa ctggacggag gaagctaaaa
     2521 atatgctgcg ggacaaggcc gttcctgtca ctgcgttaag cctgacagac cttgagcaaa
     2581 gcgcactgga ctggtcacaa ttcgattttg atccggccta caaaccggtg atgaaagcaa
     2641 aaaaacaact ccgacctcat cagactcccg cgcttgaagc cgtgaaaaga gggctgacga
     2701 ctgccgaccg cggcaagctt attatggcgt gcggcacagg gaaaaccttc acctcgctgc
     2761 gtatcgctga agcggttgcc ggacggggga aaaccgtact gttccttgtg cccagtctgg
     2821 cattgttgag tcagacgctg gatgaatgga cacaggatac gctgatagac ctgcgctgtt
     2881 ttgctgtctg ctctgacagt gatgtgggta agaaaaacca tgatgacaat gtcgttgttg
     2941 gtatcagcga cctgaaatat ccggcgacca cgaatgcgaa cagcctcgtc aaagcgttta
     3001 atcagcctga tatcttcggc agtgataagc cgccttatat gaacgtggtg ttttctacct
     3061 accactctgt tgaggtcatc catcaggcgc aaaaactggg ttttcctaca tttgatttca
     3121 tcatttgtga tgaagctcac cggaccacag gtgcgacctt tgaaggggat gatgagtccg
     3181 cctttgttcg tattcacgac aacgcctata tcgccggtca aaaacgcctg tacatgaccg
     3241 cgactccgcg cattttcggg gacgatgcga aagagactga aggggtcacc ttatgctcaa
     3301 tggatgacaa aagcctttac ggtgatgacc tgtatgtcat taccttctct aaagccgttc
     3361 agttgggcat tttgtgtgat tacaaagtta ttgtgcttgc cgtggaagaa aagcatgtca
     3421 gccgccgcat tcagtctctg ctgaaggatg aaaataatca gctcaaagtc gatgatgcgg
     3481 caaaaattgt gggttgctgg aaagcactgg caaagcaggg gctggcgggg tctgatggcg
     3541 ttcatcctga tgcgatgaag cgggcagtgg cgttctgtca ggtgatagaa aaagactttc
     3601 gcggcaaaac gcataaggtc agttctaaac tgatcactga aatgttcgga gcagtggttg
     3661 aagcctatca ggcatccgag attgaacttc tcagggaaac cgcaccggat aaggctattg
     3721 atccgtcatt aaaacttcag tgtcaggtga agcacgttga cggcagcatg aatgccactg
     3781 agaaaaaatc caaaattgaa tggctgaaag cggatacgga tgaaaatgtc tgccggatat
     3841 tgtctaacgt ccgttgttta tctgaaggtg tggatgtgcc ttctctggat gcagtgttgt
     3901 ttttaacgcc gagaagctct caggtggatg tggtgcagtc tgtcgggcgt gttatgcggc
     3961 gtgccgaagg taaagagctg ggctatgtca ttctgccggt tgtcattccc gccggagaag
     4021 agccggaaaa cgccttaaac aacaatcagg cctaccgtgt tgtctggcag gtgctgaatg
     4081 cactcagggc gcatgatgac cgctttgatg ccatgatcaa caagctggag tttaacggta
     4141 aagataccag cagaatggaa gtgatcgcgg tagccgataa agtggtgaag aaaactaaac
     4201 ggcagacgaa aaaatctgaa ctggcaggaa aagcgcgtaa aagcagtggc attggttcag
     4261 ccgtgagtcc tgttcctgag cagtttgata ttgagttttc cgttggtgag attgaacgtg
     4321 ccctgtacgc gaaaatcgtt aaaaaatgcg gtaaccggca tcactgggaa gactgggcga
     4381 acgatatcgc taaaattgcc cgcacgcata tcgaccgcat tcacggcatt ctcgaaaatc
     4441 cgggtaatca agtagaaatc agtgccttca gggcatttgc cgatgaactg cgtgatgatc
     4501 tgaataacag tgtttccgac gatgaaatca ttgaaatgct ggcacagcat ctgataacca
     4561 agccggtctt tgatgccctg tttgccgatt acaattttac cgaccataac ccgatgagta
     4621 tggcgatgca gaacgtgctt aacacgttac aggagcatca tctcgaaaaa gaagccagca
     4681 cgctgaacag cttctatgag tcggtaaaaa tgcgtgcaga cggcattacc accgcagaag
     4741 gtaaacagca gattatcgta cagttgtacg ataagttttt cagaaacgcg ttcccgagaa
     4801 tgactgagcg tctgggaatt gtgtatacgc ctgttgaggt ggttgatttt attattcaca
     4861 gcgtgaatga cgtgctgaaa aaggagttcg gacaaacact cgccgacaaa ggtgtgcata
     4921 ttcttgaccc attcaccgga accggtacgt ttatcacccg cctgctgcaa agtggtctga
     4981 tcccgtcaga taaactggct tacaagttta aaaatgaaat ccatgccaac gaaatcgtcc
     5041 tgttagccta ttacattgcc gccattaata ttgaggcggt gtatcacggc atcacggaag
     5101 aatcagagta cacgccattt gaagggatct gtctgactga cacctttgaa atgtatgaga
     5161 aagatgacct ggtcagtgag gttttggttg ataacagtga acgccgtaag cggcagaagg
     5221 cactggatat tcgggtgatt atcggtaatc caccttattc atccggtcag gaatctgcaa
     5281 acgataataa ccaaaatgta aaatatccga tgctcgataa gcggattgca gagacgtatg
     5341 ccgcttctac cactgcgacc aacaaaaatg cactctatga ttcctatatc cgtgctattc
     5401 gctgggcgag tgaccgtatc ggtgagcaag gtgtaatcgg ttttgtgact aatggtggct
     5461 atattgactc taactcggct gacgggttgc gtaagtgtct ggctgaagag ttcagtagcc
     5521 tgtatttttt ccatttgagg gggaaccagc gaacctctgg agaaaaatca cggcaggaag
     5581 gcggtaagat ttttggcagc ggtagtcgtg cgcctattgt gattgcgatt ctggtaaaaa
     5641 atccggctgc aacacagtcc ggtcagattt atttccatga tattggtgac tatctgactc
     5701 gtgaggaaaa gcttgagaag atttccgatt ttggcagtat tgacggtatt gagaaaacgc
     5761 atggctggca gaaaatcaca ccagatgcct acaatgactg gctgaatcag cgtgatgaca
     5821 gtttttatga ctttatccaa ataggcaata aaaaagataa agaaaactca tctcttttcg
     5881 atgtttattc aacagggctt cagcctagag gtgacgcatg gttttataac tactcaaaac
     5941 aaaaattagc catgaacata aaaaagtcca ttaatttttt taattctgaa gttgatcggt
     6001 ataaaaaaga atataattca gggagcttga aggactttgt aagaattgac ccatttaatt
     6061 ttgcttggta tggggaaagt tacaaagaaa tctccagaga tagatattat gatattgctg
     6121 atggtgttgt tagagtttcc ttatatagac catatagtaa gtctttggtt tactttaatg
     6181 agaacatgat gcatcgtgtt agcaaaattc ctgaaatttt cccagagaaa aactctaata
     6241 atttaattat cattattaca gggcgtggct caactaaagg gttctcttca ttaatatcaa
     6301 atgaaattgt tgatcttgag tgtatatcta aggggcaatg cttccctctc tatgtttacg
     6361 ataataaaaa acctagtatt ttgtttggca atcaagatga aagtgtgtat ttagatggtg
     6421 agtatgagcg aaaatatgcc attagtgata cagggatgat ttattttaaa aagcattatc
     6481 cggaagaaaa aataaatcag gaggatgttt tttattacat ttatggtttg ttgcacagtg
     6541 aagattatcg ctctcgctat gcggataatt tgagtaagga gttgccatat ataccatgtg
     6601 tgaaaaaaat tgaggatttt tggtcgtttt ctaaagcagg ccgtgaactt gctgagttgc
     6661 atttaaacta cgaaacagtt gagccataca aggctgatat tgatacggga tccctgagtt
     6721 attctcagtt aggtaaagac gatttttatg ttgagaagat gaagttcgcc aaaaaaggtg
     6781 aaaaagatac tgtcatttat aataataaaa ttcggataaa aaatatccct attgaagcct
     6841 atgactacat tgtgaatggt aaacctgccc ttgaatgggt gatggaacga caaggtgtat
     6901 ccacccataa ggatagtgga attgtgaatg atgccaatga ttgggcaatt gaaacaatga
     6961 ataaccctcg atacccgtta gaattgttcc tgcgagttat cactgtcagt cttgaaactc
     7021 agaaaatcgt caacagcttg ccaaaactgg atatctaatc actaggtgtg caggatggaa
     7081 aaactatcct gcacaattct gattattttc ataaattctg tagtttttaa cataatggat
     7141 cttgcgcgca ccgcccgaac acaactcgcc acaaaaaccc gccggaacgt ccaaaagtac
     7201 gggttttgct gcccgcaaac gggctgttct ggtgttgcta gtttgttatc agaatcgcag
     7261 atccggcttc agccggtttg ccggctgaaa gcgctatttc ttccagaatt gccatgattt
     7321 tttccccacg ggaggcgtca ctggctcccg tgttgtcggc agctttgatt cgataagcag
     7381 catcgcctgt ttcaggctgt ctatgtgtga ctgttgagct gtaacaagtt gtctcaggtg
     7441 ttcaatttca tgttctagtt gctttgtttt actggtttca cctgttctat taggtgttac
     7501 atgctgttca tctgttacat tgtcgagctg ttcatggtga acagctttga atgcaccaaa
     7561 aactcgtaaa agctctgatg tatctatctt ttttacaccg ttttcatctg tgcatatgga
     7621 caattttccc tttgatatgt aacggtgaac agttgttcta cttttgtttg ttagtcttga
     7681 tgcttcactg atagatacaa gagccataag aacctcagat ccttccgtat ttagccagta
     7741 tgttctctag tgtggttcgt tgtttttgcg tgagccatga gaacgaacca ttgagatcat
     7801 acttactttg catgtcactc aaaaattttg cctcaaaact ggtgagctga atttttgcag
     7861 ttaaagcata gtgtagtgtt tttcttagtc cgttatgtag gtgggaatct gatgtaatgg
     7921 ttgttggtat tttgtcacca ttcattttta tctggttgtt ctcaagttcg gttacgagat
     7981 ccatttgtct atctagttca acttggaaaa tcaacgtatc agtcgggcgg cctcgcttat
     8041 caaccaccaa tttcatattg ctgtaagtgt ttaaatcttt acttattggt ttcaaaaccc
     8101 attggttaag ccttttaaac tcatggtagt tattttcaag cattaacatg aacttaaatt
     8161 catcaaggct aatctctata tttgccttgt gagttttctt ttgtgttagt tcttttaata
     8221 accactcata aatcctcata gagtatttgt tttcaaaaga cttaacatgt tccagattat
     8281 attttatgaa tttttttaac tggaaaagat aaggcaatat ctcttcacta aaaactaatt
     8341 ctaatttttc gcttgagaac ttggcatagt ttgtccactg gaaaatctca aagcctttaa
     8401 ccaaaggatt cctgatttcc acagttctcg tcatcagctc tctggttgct ttagctaata
     8461 caccataagc attttcccta ctgatgttca tcatctgagc gtattggtta taagtgaacg
     8521 ataccgtccg ttctttcctt gtagggtttt caatcgtggg gttgagtagt gccacacagc
     8581 ataaaattag cttggtttca tgctccgtta agtcatagcg actaatcgct agttcatttg
     8641 ctttgaaaac aactaattca gacatacatc tcaattggtc taggtgattt taatcactat
     8701 accaattgag atggtctagt caatgataat tactagtcct tttcctttga gttgtgggta
     8761 tttgtaaatt ctgctagacc tttgctggaa aacttgtaaa ttctgctaga ccctctgtaa
     8821 atttcgctag acctttgtgt gttttttttg tttatattca attggttata atttatagaa
     8881 taaagaaaga atagaaaaag ataaaaaaga atagatccca gccttgtgga taactgcacg
     8941 cctgagtcac ttgagcgata tgataaaagg atgtcgcaaa cgctgtttgc ttctcttcaa
     9001 aacagacctt aaccccaaaa acactgagat agcaccctcg caagctcggg caacccgctg
     9061 aacattcctt ttgtctccga ccgtccggca cctgaatcgc tgttatctct gtgaaaatca
     9121 gttcgcttcg ctcacggctc tggcagggaa tgggggtgaa tggcactaca ggcgcctttt
     9181 atggattcat gcaaggaaac tacccataat acaagaaaag cccgtcacga gcttctcagg
     9241 gcgttttatg gcgggtctgc tatgtggtgc tatctgactt tttgctgttc agcagttcct
     9301 gccctctgat tttccagtct gaccacttcg gattatcccg tgacaggtca ttcagactgg
     9361 ctaatgcacc cagtaaggca gcggtatcat caacaggctt acccgtctta ctgtcaacca
     9421 gacccgccag gataagcaat ccggcagact ggtacagagc atggtcacgg gctttacggg
     9481 cggctctggc ttcggctcgc ttttctgcct gtatcaggtt catgactttc gctctggctt
     9541 cctgcgcttt ctctgctgcc tgttcagcct tcatcagcag
//
