LOCUS       NZ_CP063482             2347 bp    DNA     circular CON 17-JUN-2023
DEFINITION  Escherichia coli strain EM06-18-14 plasmid pEM06-18-14_3, complete
            sequence.
ACCESSION   NZ_CP063482
VERSION     NZ_CP063482.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN16387502
            Assembly: GCF_016942655.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2347)
  AUTHORS   Moser,A.I., Campos-Madueno,E.I. and Endimiani,A.
  TITLE     Complete genome and plasmid sequences of MDR Escherichia coli from
            local people and environmental sources in Laos
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2347)
  AUTHORS   Moser,A.I., Campos-Madueno,E.I. and Endimiani,A.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-OCT-2020) Institute for Infectious Diseases,
            University of Bern, Friedbuehlstrasse 51, Bern 3001, Switzerland
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Flye v. 2.7.1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 490x
            Sequencing Technology :: Illumina NovaSeq; Oxford Nanopore MiniION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 06/17/2023 06:38:14
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,887
            CDSs (total)                      :: 4,768
            Genes (coding)                    :: 4,593
            CDSs (with protein)               :: 4,593
            Genes (RNA)                       :: 119
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 175
            CDSs (without protein)            :: 175
            Pseudo Genes (ambiguous residues) :: 0 of 175
            Pseudo Genes (frameshifted)       :: 66 of 175
            Pseudo Genes (incomplete)         :: 115 of 175
            Pseudo Genes (internal stop)      :: 40 of 175
            Pseudo Genes (multiple problems)  :: 41 of 175
            Pseudo Genes (short protein)      :: 2 of 175
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP063482.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2347
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="EM06-18-14"
                     /isolation_source="chicken meat"
                     /db_xref="taxon:562"
                     /plasmid="pEM06-18-14_3"
                     /geo_loc_name="Laos"
                     /collection_date="2018"
                     /collected_by="Institute for Infectious Diseases Bern"
     gene            join(2139..2347,1..13)
                     /locus_tag="IQS22_RS24175"
                     /old_locus_tag="IQS22_24185"
     CDS             join(2139..2347,1..13)
                     /locus_tag="IQS22_RS24175"
                     /old_locus_tag="IQS22_24185"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_205561698.1"
                     /translation="MGAKFLDQKTGVILEEPEFVKLYIRDLCRVKGLSATQYKIFTFML
                     ANMNTDNVVSFGPRTKEKFLSEHSITFN"
                     /name="hypothetical protein"
     gene            complement(305..730)
                     /gene="mobA"
                     /locus_tag="IQS22_RS24180"
                     /old_locus_tag="IQS22_24190"
     CDS             complement(305..730)
                     /gene="mobA"
                     /locus_tag="IQS22_RS24180"
                     /old_locus_tag="IQS22_24190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001107325.1"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization protein MobA"
                     /protein_id="WP_039023338.1"
                     /translation="MTKNKRSGSEVRQRTKVITLRVNNRIANEIRRRAKSEGLTISEYI
                     RTTSLNNEIKQRVPSRYLDELIQLGRMQKKLFDKGKRPKDKEYLDVMHKIISLCAEMKE
                     VTQRISDIYKKMDLIKEEIKAIKKIHKNKYPGSNLFN"
                     /name="mobA"
     Typing          complement(1514..1772)
                     /name="Col(MG828)"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="84.528"
                     /coverage="100.0"
                     /evalue="1.5e-65"
ORIGIN
        1 aacattcaat taaaccacaa acatttaata ataatgtgtc tcaactgatt gagtcatgtt
       61 taatttctag aatagccaga aatgaatttt tggttaataa aaaatacgca gttaaagttg
      121 attgggcgaa agtacaaaag atagtatgga caacaactta cagtaatgaa gggattgatt
      181 caacagtgac gatagccccg aaaaaaacgg attaatttct tatgattgta aaagatttcg
      241 ataaaacaaa atgtcgctaa ttctattata ggtatttaaa tttttatgaa gacctatgtt
      301 ctgctcaatt aaataagttg gaacctgggt atttattttt atgtattttt tttattgctt
      361 ttatttcttc ttttattaaa tccatttttt tatatatatc agaaattcgc tgtgttactt
      421 ctttcatttc agcacaaagt gaaattattt tatgcatcac atccagatac tctttgtcct
      481 tcggtctctt ccctttatca aataatttct tctgcatccg accaagttgg attaattcat
      541 ctaaatatct ggaaggaacg cgctgtttta tttcattatt taagcttgtt gtccttatat
      601 attctgaaat agtcagccct tcgctcttcg ctcgccgtct gatttcgttt gcgattctgt
      661 tatttactct aagggtgata accttagtcc tctgccgtac ttctgaaccg ctacgcttat
      721 ttttagtcat cgcaatgatt ctccattttg cacgtttggg gtgtcggggc ttgccctgac
      781 aggaggtatt ctcatgcaag cgaatgtatt cgcggtatga cgaatacaca tcctgtccca
      841 ttttccgctg caacttttcg acgaaaataa aataccgcgt cgtaacctgg tatttctgat
      901 acaaaaaaat gatgcttcat tcaatattat ttacacagat tttaaaaatt attgcttgtt
      961 atttaataaa taacaccgaa ttaattgatg tctttgttgt ctgtcgtttg tggattatta
     1021 gaggaataaa gattagatct tcttggttat ataatccatt gcctgttggt aatgcgggat
     1081 tcgtccagag agcaggaggc aaaagagcaa gatggtagca tatgcataaa atataactat
     1141 gtgagttatt gatatcgcgt ttatatatta aaaatataac ttaacttcgc tcctaatctt
     1201 tatttcatcc tagcaatggg agcattaatc cccgtgcagt gctccgccag ctgctaacaa
     1261 cctgaaaggc aaaagacaca acaaacttgc ctgacacaga aaagcgcgct acaaagcgtc
     1321 tgagcgcgtt tcctcctcgt tcaatctgtt cagttcagtc atagcttgcc tggaaaacct
     1381 cttggaaacg cgctcaccct tcaccatcga agccgccacg gttccggcag cgccttgagc
     1441 gcaaccggaa accgtctggg gagaacccca gacccccatc gctgtaacac cgacggtcta
     1501 cgaccaccta ccaaccgagt ggctgtctgt tccgtgcaaa tgggccttgc agggggctgg
     1561 cgcggttcct agcgacctga aagcccctgg ttaggggtat cggcgcgcct aaccttctga
     1621 aaacctctgt aagcgttttt cgcgccttcc atgccctgac gggcatttaa cccccgtttt
     1681 gtgcgtctgg gaggctttga gcgcgtttaa ccggtggcca gcgactcgat cgcctgagat
     1741 caaatcggga tcgtcaacga tatgcgagag gtggcaccgc tcgccgcagc cccagcgacc
     1801 gagccgctag gctcgcgagt gggcgaggaa gcggaaggga accagtattg gcatagtgca
     1861 cttacgcacg agatctaaga tagattcaca gacagatagg aaaatcaggt gtgacaaggc
     1921 ctagaggcaa actaaatacc gctcagcggg aatagatata ccgctcagcg ggaaagtttt
     1981 taccgctgag cggaggttga aataccgctc agcggtattt caaataccgg aatactgttt
     2041 ttgttgctaa tcaaataccg gaaaaaaagt atttactcgg ttgtggaaca tacataagat
     2101 accgataaaa cggtaactaa attacctgcg aggtgagaat gggagcgaaa tttttagatc
     2161 aaaaaactgg cgtaattttg gaagagccgg agttcgtgaa gctgtatatc cgtgatttat
     2221 gccgtgttaa agggctatca gcaacgcaat acaaaatttt tacgttcatg cttgctaata
     2281 tgaatactga taatgttgtt tcatttggac caagaaccaa agaaaagttt ttatcagaac
     2341 attcaat
//
