LOCUS       NZ_CP063472            29186 bp    DNA     circular CON 17-JUN-2023
DEFINITION  Escherichia coli strain EM20-18-50 plasmid pEM20-18-50_5, complete
            sequence.
ACCESSION   NZ_CP063472
VERSION     NZ_CP063472.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN16387510
            Assembly: GCF_016942595.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 29186)
  AUTHORS   Moser,A.I., Campos-Madueno,E.I. and Endimiani,A.
  TITLE     Complete genome and plasmid sequences of MDR Escherichia coli from
            local people and environmental sources in Laos
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 29186)
  AUTHORS   Moser,A.I., Campos-Madueno,E.I. and Endimiani,A.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-OCT-2020) Institute for Infectious Diseases,
            University of Bern, Friedbuehlstrasse 51, Bern 3001, Switzerland
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Flye v. 2.7.1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 375x
            Sequencing Technology :: Illumina NovaSeq; Oxford Nanopore MiniION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 06/17/2023 06:49:07
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,592
            CDSs (total)                      :: 4,474
            Genes (coding)                    :: 4,235
            CDSs (with protein)               :: 4,235
            Genes (RNA)                       :: 118
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 66 of 239
            Pseudo Genes (incomplete)         :: 169 of 239
            Pseudo Genes (internal stop)      :: 47 of 239
            Pseudo Genes (multiple problems)  :: 43 of 239
            Pseudo Genes (short protein)      :: 4 of 239
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP063472.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..29186
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="EM20-18-50"
                     /isolation_source="chicken meat"
                     /db_xref="taxon:562"
                     /plasmid="pEM20-18-50_5"
                     /geo_loc_name="Laos"
                     /collection_date="2018"
                     /collected_by="Institute for Infectious Diseases Bern"
     gene            complement(545..1516)
                     /locus_tag="IQS25_RS22525"
                     /old_locus_tag="IQS25_22545"
     CDS             complement(545..1516)
                     /locus_tag="IQS25_RS22525"
                     /old_locus_tag="IQS25_22545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000817038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family plasmid partition protein"
                     /protein_id="WP_000817038.1"
                     /translation="MKRAPVIPRHTTHTQSTEDTSSPAPAAPMVDSLIARVGAMARGNA
                     ISLPVCGREVKFTLEVLRGDSVESASRVWSGNERDQELLTEDALDDLIPSFLLTGQQTP
                     AFGRRVSDVIEIADGSRRRKAAILTESDYRVLVGELDDEQMAALSRLGNDYRPTSAYER
                     GLRYTSRLQNEFAGNISALADAENISRKIITRCINTAKLPKSVVALFAHPGELSARSGE
                     ALQKAFADKEELLKQQAETLHDQKKAGLIFEAEEVISLLTSVLKQSPASRVNLSSRHQF
                     APGATALYKGDKMVLNLDRSRIPAECIEKIEAILKELEKPGV"
                     /name="ParB/RepB/Spo0J family plasmid partition protein"
     gene            complement(1516..2682)
                     /gene="sopA"
                     /locus_tag="IQS25_RS22530"
                     /old_locus_tag="IQS25_22550"
     CDS             complement(1516..2682)
                     /gene="sopA"
                     /locus_tag="IQS25_RS22530"
                     /old_locus_tag="IQS25_22550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid-partitioning protein SopA"
                     /protein_id="WP_000523812.1"
                     /translation="MGLMDTLNQCISAGHEMTKAIAIAQFNDDSPEARKITRRWRIGEA
                     ADLVGVSSQAIRDAEKAGRLPHPDMETRGRVEQRVGYTIEQINHMRDVFGTRLRRAEDA
                     FPPVIGVAAHKGGVYKTSVSVHLAQDLALKGLRVLLVEGNDPQGTASMYHGWVPDLHIH
                     AEDTLLPFYLGEKDDASYAIKPTCWPGLDIIPSCLALHRIETELMGKFDEGKLPADPHL
                     MLRLAIETVAHDYDVIVIDSAPNLGIGTINVVCAADVLIVPTPAELFDYTSALQFFDML
                     RDLLKNVDLKGFEPDVRILLTKYSNNNGSQSPWMEEQIRDAWGSMVLKNVVRETDEVGK
                     GQIRMRTVFEQAIDQRSSTGAWRNALSIWEPVCNEIFDRLIKPRWEIR"
                     /name="sopA"
     gene            3423..4433
                     /locus_tag="IQS25_RS22535"
                     /old_locus_tag="IQS25_22555"
     CDS             3423..4433
                     /locus_tag="IQS25_RS22535"
                     /old_locus_tag="IQS25_22555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017900946.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="WP_000200069.1"
                     /translation="MTSENNSLLLNLQEVDKTTGEVVKLDVNSTSTVQPVALMRLGLFV
                     PTLKSTGKSKANRKNVTDATEELVQLAIAKSEGYTDVKITGSRLDMDTDFKVWLGIIRS
                     MSEYGVKSDTLELSFVEFVKMCGFDSRRSNKKMRDRISNSLFKLASVTLKFQSETKGWT
                     THLVQSAYYDINEDIVEIKAEPKLFELYHMDRRVLLRLKAIDALQRKESAQALYTYIES
                     LPQNPAPISMKRMRDRLNLTSNVYTQNHTVRKAMEQLRDIGYLDYTEFKRGRATYFSVH
                     YRNPKLISSPVKVPRKEEEEKAPEQNYDEVIKALKAAGIDPLKLAEALSAMKPEN"
                     /name="RepB family plasmid replication initiator protein"
     gene            complement(5070..5810)
                     /locus_tag="IQS25_RS22540"
                     /old_locus_tag="IQS25_22560"
     CDS             complement(5070..5810)
                     /locus_tag="IQS25_RS22540"
                     /old_locus_tag="IQS25_22560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012540156.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0015074 - DNA integration [Evidence IEA];
                     GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="WP_072647229.1"
                     /translation="MNSIIPLQNSPERVSLLPIAPGVDFATAVALRRMATSTGVTPAYL
                     LAPEVSALLWYMPDQRHHMLFATMWNTGIRIGEARTLTPESFDLDGLRPFVRVLSEKVR
                     ARRGRPPKDEVRLVPLTDASFVRQMESWMVTTRPRRREPLWPVTDETMRNWLKQAVKRA
                     EADGVHFSIPVTPHTFRHSYIMHMLYHRQPRKVIQALAGHKDPRSMEVYTRVFALDMAA
                     TLAVPFTGDGRDAAEILRSLPPAG"
                     /name="tyrosine-type recombinase/integrase"
     gene            complement(6393..7090)
                     /locus_tag="IQS25_RS22545"
                     /old_locus_tag="IQS25_22565"
     CDS             complement(join(6393..6841,6841..7090))
                     /locus_tag="IQS25_RS22545"
                     /old_locus_tag="IQS25_22565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558153.1"
                     /ribosomal_slippage=""
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="WP_232210070.1"
                     /translation="MVSISIRYPSCSATEGVVRNGKSTAGHQRYLCSHCRKTWQLQFTY
                     TASQPGTHQKIIDMAMNGVGCRASARIMGVGLNTVLRHFKKLRPQSVTSRIQPGSDVIV
                     CAEMDEQWGYVGAKSRQRWLFYAYDRIRRTVVAHIFGERTLATLERLLSLLSAFEVVVW
                     MTDGWPLYESRLKGKLHVISKRYTQRIERHNLNLRQHLARPGRKSLSFSKSVELHDKVI
                     GHYLNIKHYQ"
                     /name="IS1 family transposase"
     gene            complement(7141..7365)
                     /locus_tag="IQS25_RS22550"
                     /old_locus_tag="IQS25_22570"
     CDS             complement(7141..7365)
                     /locus_tag="IQS25_RS22550"
                     /old_locus_tag="IQS25_22570"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001142363.1"
                     /translation="MPTITAKVSDELLAYIDLVSGGNRSEYLRRCLEAGPGDLESGLKI
                     VTNQLSDVNRKLDYLFDRASDADLGSLRG"
                     /name="hypothetical protein"
     gene            7887..8222
                     /gene="stbA"
                     /locus_tag="IQS25_RS22555"
                     /old_locus_tag="IQS25_22575"
     CDS             7887..8222
                     /gene="stbA"
                     /locus_tag="IQS25_RS22555"
                     /old_locus_tag="IQS25_22575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006796897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid stabilization protein StbA"
                     /protein_id="WP_001039462.1"
                     /translation="MNKPADLKPRNLSAAVRLRLNEIENWLDRGLTRHEIAEILDSEYS
                     FSVTAKGLEMALYRTRKNRKNVLYNTQPSEPEAQESEKAESPGIIDKEFFNKIGEDFNP
                     KKFNKKF"
                     /name="stbA"
     gene            8231..8422
                     /locus_tag="IQS25_RS22560"
                     /old_locus_tag="IQS25_22580"
     CDS             8231..8422
                     /locus_tag="IQS25_RS22560"
                     /old_locus_tag="IQS25_22580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000861580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000861578.1"
                     /translation="MKVAVINYSGSVGKTLISSYLLAPRLTGAKFYAVETINQSASDLG
                     IENVTSFKGDDFSRLRLC"
                     /name="hypothetical protein"
     gene            8444..9412
                     /locus_tag="IQS25_RS22565"
                     /old_locus_tag="IQS25_22585"
     CDS             8444..9412
                     /locus_tag="IQS25_RS22565"
                     /old_locus_tag="IQS25_22585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611102.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="WP_077896987.1"
                     /translation="MKDQITHLPDNADRSVAKQKFKITNWPTYNKALINRGSITFWLDD
                     EAIQAWYESTTPSSRGRPQRYSDLTITTVLVIKRVFRLTLRAAQGFIDSIFALMNVPLR
                     CPDYTSVSKRAKSVNVSFKTFTRSEIAHLVIDSTGLKVFGEGEWKVKKHGKERRRIWRK
                     LHLAVDSKTHEIICADLSLNNVTDSEAFPGLIRQTHRKIRAASADGAYDTRLCHDELRR
                     KKISALIPPRKGAGYWPGEYADRNRAVANQRMTGSNARWKWTTDYNRRSIAETAMYRVK
                     QLFGGSLTLRDYDGQVAEAMALVRALNKMTKAGMPESVRIA"
                     /name="IS5 family transposase"
     gene            9727..9993
                     /locus_tag="IQS25_RS22570"
                     /old_locus_tag="IQS25_22590"
     CDS             9727..9993
                     /locus_tag="IQS25_RS22570"
                     /old_locus_tag="IQS25_22590"
                     /inference="COORDINATES: protein motif:HMM:NF012464.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="WP_241415787.1"
                     /translation="MGEMYYSLIAIFSNFEVARNHERTMAGLAAAKKKGRIGGRKPKLS
                     PQDIKEINALVKSPEITVKEIAARFKVSRPTIYRHISSDIQDE"
                     /name="recombinase family protein"
     gene            complement(10065..10787)
                     /locus_tag="IQS25_RS22575"
                     /old_locus_tag="IQS25_22595"
     CDS             complement(10065..10787)
                     /locus_tag="IQS25_RS22575"
                     /old_locus_tag="IQS25_22595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012561120.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_072647141.1"
                     /translation="MDKILNKKVNVKQANELTEAAYYLSLKAKRVLWLCLMQTYFTASS
                     TDDSDKPDVVLGDAVFKVSVAEYATVFGVTRNQAVKDVKDGIIELSRSSVVFYPKEGRY
                     DCVARPWLTEAGNRSTRGVWEIEFNHKLLPYIYGLTRQFTTYSLHDCGSLRNPRTIRLY
                     ESLCQFKSTGLWITNHAWLNERFVLPESQQKNLAELRRSFLDPALKQINEKTPLKASYS
                     IDSNGKFLFSVINKPLAA"
                     /name="replication initiation protein"
     gene            11379..>11582
                     /locus_tag="IQS25_RS22580"
                     /old_locus_tag="IQS25_22600"
                     /pseudo=""
     CDS             11379..>11582
                     /locus_tag="IQS25_RS22580"
                     /old_locus_tag="IQS25_22600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612015.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /name="transposase"
     gene            complement(11592..12805)
                     /locus_tag="IQS25_RS22585"
                     /old_locus_tag="IQS25_22605"
     CDS             complement(join(11592..12511,12511..12805))
                     /locus_tag="IQS25_RS22585"
                     /old_locus_tag="IQS25_22605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612254.1"
                     /ribosomal_slippage=""
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="WP_104693046.1"
                     /translation="MIFSPQHKTGDLMNKKTKRTFTPEFRLECAQLIVDKGYSYRQVSE
                     AMNVGSTTLESWVRQLRRERQGITPSATPITPDQQRIRELEKQVRRLEEQNTIFKKGYR
                     ALNVRLAERFTIAARLSDSHTVVSLCSALEIHRSSYRYWRKRRDTVNPARVRLCSEIRR
                     AWNQSRGSAGARTLAEMLTQNGVPMSRYRAGRLMKYLNLSSCQPGKHQYKNARQEHTSL
                     PNLLERQFAVPEPDRVWCGDITYLWAGNRWCYLAVVMDLFARRVIGWSLSAHADTALIS
                     SALRMAYETRGQPRDVVFHSDQGSQYTGLKYQQLLWRCRINQSVSRRGNCWDNSPMERF
                     FRSLKTEWVPTNGYAGKDEARQQINDYILNYYNSVRPHHYNGGLTPEESENRYHFYCKT
                     VANIT"
                     /name="IS3 family transposase"
     gene            complement(<12843..12941)
                     /locus_tag="IQS25_RS22590"
                     /old_locus_tag="IQS25_22610"
                     /pseudo=""
     CDS             complement(<12843..12941)
                     /locus_tag="IQS25_RS22590"
                     /old_locus_tag="IQS25_22610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000807689.1"
                     /note="RepE; RepFIB; incomplete; partial in the middle of a
                     contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /name="RepB family plasmid replication initiator protein"
     gene            13475..14172
                     /locus_tag="IQS25_RS22595"
                     /old_locus_tag="IQS25_22615"
     CDS             join(13475..13724,13724..14172)
                     /locus_tag="IQS25_RS22595"
                     /old_locus_tag="IQS25_22615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743396.1"
                     /ribosomal_slippage=""
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1-like element IS1A family transposase"
                     /protein_id="WP_224053102.1"
                     /translation="MASISISCPSCSATEGVVRNGKSTAGHQRYLCSHCRKTWQLQFTY
                     TASQPGTHQKIIDMAMNGVGCRATARIMGVGLNTILRHFKKLRPQSVTSRIQPGSDVIV
                     CAEMDEQWGYVGAKSRQRWLFYAYDRLRKTVVAHVFGERTMATLGRLMSLLSPFDVVIW
                     MTDGWPLYESRLKGKLHVISKRYTQRIERHNLNLRQHLARLGRKSLSFSKSVELHDKVI
                     GHYLNIKHYQ"
                     /name="IS1-like element IS1A family transposase"
     gene            complement(14335..17622)
                     /locus_tag="IQS25_RS22600"
                     /old_locus_tag="IQS25_22620"
     CDS             complement(14335..17622)
                     /locus_tag="IQS25_RS22600"
                     /old_locus_tag="IQS25_22620"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="WP_251301898.1"
                     /translation="MAITVISFFKKFLTEIIPDFKNALIAALQKQNDIECILTICNCPS
                     LNAAAEKYNIPVINLELGPLRSPTYLFTGYFDFSGVNGNTEAEKRYFAAKQQGVVFNKK
                     NRISALRDFFINSRNDDDYIEPEYDVGIVLQVENDSNILAFSNSFDNQALLDYAEGSFI
                     GKKIVRPHPGSHFRLIENLGYEIDNNNLSIIDFLKKCRKILTINSSVGLEAMLMNIPVK
                     ILGDCSYAFCNVDDIDERVERLTFYLFSYLVPFDLLFNAEYIRFRLTFPSEHEILSKHI
                     LYYLSSREKQSIIDMKTSTTDVVENGMYERGLLKELEELKIKNIELNDIVSRLELELQQ
                     EKLLQAENDNNDILVDVIVPVYGGLQETQECIESAISTLPSWAELIVINDASPEPELTD
                     WLRKRAQDNSFKLLENENNLGFVATVNRGMKLHSKRDVLLLNSDVEVANKWLERIYDAA
                     YSRERVGSVTPFSNNATICSFPNFCEDNELFSGMDVQALDDEFETYGKENNLVEIPTGV
                     GFCMYIRRDCLNQVGYFDEETFGRGYGEENDWCQRAAKAGWPNFHQLNVFVYHKGGVSF
                     AGEQSPRKIRAMELLNELHPNYTRDVMEFISRDPAKQARQRMLLRILAKANLQKILLVS
                     HHMGGGTTYHLEELSNFYKGRACFFILKPNADNSSVVISLSADGRTCKESISVDVENGY
                     DDLLRLLRFLGISHIHFHHLMGIPEKIKKLHLDLKCSYDVTIHDYFAINGNPSLTDKNG
                     LFIGDNTTNMDELCAETYPLPVGMSAATWRSNMNAWLFGAERVIFPSADTKFRFTRYFP
                     DISEKAVIAWHPDYEKNAPYPDVRKTYTGDRKLKVLVLGAISREKGAFMLNEVAHALKR
                     ENIEFHLLGYACSTLNDAVICHGAYSNQDLEKKLNDIAPDVMWFPAQWPETYSYTLSAA
                     LQYGLPVVVPNIGAFGERLIGRKFSRIINWDISFQRMSELWRQAIADFDGFFQQQNVDP
                     MPNVSSETRVDHFYQDYYIRDSWWRESELGRIDFKTLIDSFYSSENITIAEEKISQPHG
                     RKEKLLAVLWMLRQNPALSWATKLIPYRLQRYVKRQLSRRALHEIVR"
                     /name="glycosyltransferase"
     gene            complement(17594..17902)
                     /locus_tag="IQS25_RS23000"
     CDS             complement(17594..17902)
                     /locus_tag="IQS25_RS23000"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_251301897.1"
                     /translation="MIGIYLPAFPFRGVKAPYLWFLYKAMTAIKEPLALITTQDYLLPC
                     TKWQEEGRWEVTDDGQKRLGYELPNFSVRQKDNVYIVNEESLSEALTECGDNSNLLF"
                     /name="hypothetical protein"
     gene            complement(17899..19230)
                     /locus_tag="IQS25_RS22605"
                     /old_locus_tag="IQS25_22625"
     CDS             complement(17899..19230)
                     /locus_tag="IQS25_RS22605"
                     /old_locus_tag="IQS25_22625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015958690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="WP_063930273.1"
                     /translation="MSSNQIAIKVTNLSKCYQIYSQPWDRLKQFFAPKIKSVIRRESRS
                     YFKEFWALRDVSFSINRGETVGIIGRNGAGKSTLLQMICGTLTPTVGDIEVNGRVAALL
                     ELGAGFNPEFSGRDNVYLNGSVLGLSKSEIDKKFQEILDFADIGDFIDQPVKTYSSGMY
                     VRLAFAVQACIDPEILIVDEALAVGDIGFQYKCFKRMEMLKQKGTTIIMVTHSTGSILE
                     YADRCLVMSDGMLVGNTTDVLAAVLAYEKGMLMTERTDTAEKKEAVSQTGENASADELS
                     AIQNTQTNAEVDEKRFGSGRAIIEDFSIMKADGTPLSEKPLVKPNEEIYFSFKLNSSEN
                     INDVVLGVSLSRTQGGDIWGDNNIFAGHKIDLAPGLRKLKYKVKLPINTGDYLVHCGLA
                     CFNNGEREELDQRRPIAKVKFWSSRELGGVIHSPVNVIEGDELS"
                     /name="ABC transporter ATP-binding protein"
     gene            complement(19220..20053)
                     /locus_tag="IQS25_RS22610"
                     /old_locus_tag="IQS25_22630"
     CDS             complement(19220..20053)
                     /locus_tag="IQS25_RS22610"
                     /old_locus_tag="IQS25_22630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004942430.1"
                     /GO_component="GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /GO_function="GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="WP_065799755.1"
                     /translation="MKNPHQKPSLSPLDIVKSIYTHRSIILQMTKRDVIGRYKGSVMGV
                     LWSFLNPLFMLTVYTFVFSVVFKARWSTGGDESRTQFAIILFVGMIVHGFFGEVINRAP
                     QIILNNTNYVKKVIFPLETLSVISLVAALFHTAISLLVLLTAFVIFNGFIHWTIIFIPL
                     VFLPLVIFSLGLSWILASLGVFLRDVSQTTVIITTVLMFLSPVFFPISALPEKYHIWIM
                     LNPLTFIIEQARTVLIWGGGPNFMGILLYTVGASIVAWLGFVFFQKTRKGFADVL"
                     /name="ABC transporter permease"
     gene            complement(20082..20912)
                     /locus_tag="IQS25_RS22615"
                     /old_locus_tag="IQS25_22635"
     CDS             complement(20082..20912)
                     /locus_tag="IQS25_RS22615"
                     /old_locus_tag="IQS25_22635"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015958692.1"
                     /GO_function="GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="WP_039022298.1"
                     /translation="MTYKAMKPKVIASIVLFNHSYDDIKDTLLSLCHEESVEKIIFVDN
                     GGCQWAASLNEPKVSYIKSPRNCGFGAGHNLAIKASSDFDGYFLICNPDISFDKQSLDK
                     LVSFAWINEYNFVSPQIIYKNGERQYNCRLLPTPSNLFLRRFLPTTAIKYDATYELKDA
                     EYDKVFSPPSVSGCFMLLTNVLLQKLNGFDERYFMYLEDVDLCRRALQLTKIYYCPETT
                     IVHVFNKGSYKSKLLLWYHIRSAITYFNKWGWFFDKKRYAYNESALRNIPRKLS"
                     /name="glycosyltransferase family 2 protein"
     gene            complement(20999..21553)
                     /gene="rfbC"
                     /locus_tag="IQS25_RS22620"
                     /old_locus_tag="IQS25_22640"
     CDS             complement(20999..21553)
                     /gene="rfbC"
                     /locus_tag="IQS25_RS22620"
                     /old_locus_tag="IQS25_22640"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015958693.1"
                     /GO_function="GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA]"
                     /GO_process="GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]; GO:0009243 - O antigen biosynthetic
                     process [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="WP_063930275.1"
                     /translation="MNIIKTEIPDVMIFEPRVFGDARGFFFESFSSKVFNEAVGRQVEF
                     VQDNHSQSQKGVLRGLHYQLDPHAQGKLVRCVEGEVFDVAVDIRRSSPTFGKWVGAVLS
                     AENKRQLWIPEGFAHGFMALSDTVQFVYKATNYYAPQSERSIIWNDPEIGIDWPSLSDC
                     ALSLSEKDLQAHTLATAEVYA"
                     /name="rfbC"
     gene            complement(21568..22458)
                     /gene="rfbD"
                     /locus_tag="IQS25_RS22625"
                     /old_locus_tag="IQS25_22645"
     CDS             complement(21568..22458)
                     /gene="rfbD"
                     /locus_tag="IQS25_RS22625"
                     /old_locus_tag="IQS25_22645"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="WP_063930276.1"
                     /translation="MKILLIGKNGQVGWELQRSLSTLGDVVAVDYFDKELCGDLTNLDG
                     IAQTVRTVRPDVVVNAAAHTAVDKAESERELSDLLNDKGVAVLATESAKLGALMVHYST
                     DYVFDGAGSHYRREDEATGPLNVYGETKRAGELALEQGNPRHLIFRTSWVYATRGANFA
                     KTMLRLAGEKETLSIIDDQHGAPTGAELLADCTATAIRETLRDPALAGTYHLVASGETS
                     WCDYARYVFEVARAHGAELAVQEVKGIPTTAYPTPAKRPLNSRLSNEKFQQAFGVTLPD
                     WRQGVARVVTEVLGK"
                     /name="rfbD"
     gene            complement(22490..23356)
                     /gene="rfbA"
                     /locus_tag="IQS25_RS22630"
                     /old_locus_tag="IQS25_22650"
     CDS             complement(22490..23356)
                     /gene="rfbA"
                     /locus_tag="IQS25_RS22630"
                     /old_locus_tag="IQS25_22650"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016161475.1"
                     /GO_function="GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA]"
                     /GO_process="GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]; GO:0009243 - O antigen biosynthetic
                     process [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="WP_039022301.1"
                     /translation="MKGIVLAGGSGTRLYPITQGVSKQLLPIYDKPMIFYPVSVLMLAG
                     IRDILIISTPDDMPSFQRLLGDGSQFGVNFSYAVQPSPDGLAQAFIIGEKFIGNDACAL
                     VLGDNIYFGQSFGKKLEAAAKTSGATVFGYQVLDPERFGVVEFDDNFKALSIEEKPLKP
                     KSDWAVTGLYFYDNNVVEMAKDVKPSERGELEITTLNQMYLERGDLQVELLGRGFAWLD
                     TGTHDSLIEASQFIHTIEKRQGMKVACLEEIAYRNQWLSAEGVAAQAERLKKTEYGAYL
                     KRLLNER"
                     /name="rfbA"
     gene            complement(23370..24434)
                     /gene="rfbB"
                     /locus_tag="IQS25_RS22635"
                     /old_locus_tag="IQS25_22655"
     CDS             complement(23370..24434)
                     /gene="rfbB"
                     /locus_tag="IQS25_RS22635"
                     /old_locus_tag="IQS25_22655"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227858.1"
                     /GO_function="GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA]"
                     /GO_process="GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]; GO:0009243 - O antigen biosynthetic
                     process [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="WP_039022302.1"
                     /translation="MKILVTGGAGFIGSAVVRHIIENTRDEVRVLDCLTYAGNLESLAP
                     VAGSERYSFSQTDITDAAAVAAQFSEFRPDIVMHLAAESHVDRSIDGPAAFIQTNVIGT
                     FTLLEAARHYWSGLGDAQKQAFRFHHISTDEVYGDLHGTDDLFTEETPYAPSSPYSASK
                     AGSDHLVRAWNRTYGLPVVVTNCSNNYGPYHFPEKLIPLTILNALAGKPLPVYGNGEQI
                     RDWLYVEDHARALYKVATEGKSGETYNIGGHNERKNIDVVRTICAILDKVVAQKPGNIT
                     HFADLITFVTDRPGHDLRYAIDAAKIQRDLGWVPQETFESGIEKTVHWYLNNQTWWQRV
                     LDGSYAGERLGLNN"
                     /name="rfbB"
     gene            complement(24950..26178)
                     /locus_tag="IQS25_RS22640"
                     /old_locus_tag="IQS25_22660"
     CDS             complement(join(24950..25863,25863..26178))
                     /locus_tag="IQS25_RS22640"
                     /old_locus_tag="IQS25_22660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558215.1"
                     /ribosomal_slippage=""
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEc36 family transposase"
                     /protein_id="WP_162842360.1"
                     /translation="MIDVLGPEKRRWRSVQEKIAIVQQSFEPGMTVSLVARQHGVAASQ
                     LFLWRKQYQEGSLTAVAAGEQVVPTSELASAMKQIKELQRLLGKKTMENELLKEAVEYG
                     RPKKVDSARALVAGGWRISLVSRCLRVSRAQLHAMARRSKDWQDRRCKRKPDDTDALAR
                     IHTVIGDLPTYGYRRVWALLRRQSETDDMAVINAKRVYRIMRQNALLLERKPEIPPSKR
                     AHTGKVTVGESNQRWCSDGFEFSCDNGEKLRVTFALDCCDREALYWAASNGGYDSETVQ
                     DVMLGAVERRFGNSLPTSPVEWLTDNGSAYRSYQTRQFARMVGLEPKHTAVRSPESNGM
                     AESFVKTMKRDYISIMPKPDGLTAVKNLAEAFEHYNEWHPHSALGYRSPREYLRRRTSN
                     GLSDKKCMEI"
                     /name="IS3-like element ISEc36 family transposase"
     gene            complement(26574..27271)
                     /locus_tag="IQS25_RS22645"
                     /old_locus_tag="IQS25_22665"
     CDS             complement(join(26574..27022,27022..27271))
                     /locus_tag="IQS25_RS22645"
                     /old_locus_tag="IQS25_22665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558270.1"
                     /ribosomal_slippage=""
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="WP_250306268.1"
                     /translation="MASISISCPSCSATEGVVRNGKSTAGHQRYLCSHCRKTWQLQFTY
                     TASQPGTHQKIIDMAMNGVGCRASARIMGVGLNTILRHFKKLRPQSVNSRIQPGSDVIV
                     CAEMDEQWGYVGAKSRQRWLFYAYDRIRRTVVAHVFGERSLATLERLPGLLSAFEVVVW
                     MTDGWPLYESRLKGKLHVISKRYTQRIERHNLNLRQHLARPGRKSLSFSKSVELHDKVI
                     GHYLNIKHYQ"
                     /name="IS1 family transposase"
     gene            complement(27540..>28067)
                     /gene="repE"
                     /locus_tag="IQS25_RS22650"
                     /old_locus_tag="IQS25_22670"
                     /pseudo=""
     CDS             complement(27540..>28067)
                     /gene="repE"
                     /locus_tag="IQS25_RS22650"
                     /old_locus_tag="IQS25_22670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000807689.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein RepE"
                     /name="repE"
     gene            <28068..28274
                     /locus_tag="IQS25_RS22655"
                     /old_locus_tag="IQS25_22675"
                     /pseudo=""
     CDS             <28068..28274
                     /locus_tag="IQS25_RS22655"
                     /old_locus_tag="IQS25_22675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004389801.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /name="IS30 family transposase"
     gene            complement(<28287..28589)
                     /locus_tag="IQS25_RS22660"
                     /old_locus_tag="IQS25_22680"
                     /pseudo=""
     CDS             complement(<28287..28589)
                     /locus_tag="IQS25_RS22660"
                     /old_locus_tag="IQS25_22680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612262.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0004803 - transposase activity [Evidence IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /name="transposase"
     Typing          complement(7428..7684)
                     /name="MOBF"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="81.579"
                     /coverage="96.0"
                     /evalue="1.58e-50"
     Typing          3424..4433
                     /name="IncFIB"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="90.307"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(10066..10787)
                     /name="rep_cluster_1760"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="98.893"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 cgatgcaggt gggaccgtgg tcccaggcag ataacctgac cggcgataca ggtaggaccg
       61 tggtcccagg cagataacct gacagacgat gcaggtggga ccgtggtccc aggcagataa
      121 cctgaccggc gatacaggtg ggaccgtggt cccaggcaga taagctgacc ggcgatacag
      181 atgagaccgt ggtcccaggc agataacctg accggcgata caggtgggac cgtggtccca
      241 ggcagataag ctgaccggcg atacagatga gaccgtggtc ccaggcagaa aagctgaccg
      301 gcgatgcagg tgggaccgtg gtcccaggca gataacctga ccggcgatac aggtgggacc
      361 gtggtcccag gcagataagc tgaccgacga cgcaggtggg accgtggtcc cagggagata
      421 acctgagcgg cgatacagat gggaccgtgg tcccagagag aatattcagg ccagttatgt
      481 tttctggcct gtgagaaagg aaatgaagta aagggagatc accgtagact aaagcgtgtc
      541 cgcatcagac tcctggtttc tcaagctcct taagaatggc ttctattttc tctatacact
      601 ccgcaggaat gcgggaccta tccaggttaa gcaccatttt atcgccctta tacaatgctg
      661 ttgcccctgg agcaaactga tgacgtgagc tcagattaac tcttgatgcg ggagattgtt
      721 tcagtacgga tgttaaaaga ctaatgacct cttcagcttc aaagattagt cccgctttct
      781 tctgatcgtg gagagtctca gcctgctgct tcagtaattc ttctttatct gcaaaagcct
      841 tctgaagggc ttcacctgac cgggcagaca gttctccagg atgtgcaaac agggcaacaa
      901 cggatttagg caatttggcc gtattgatac agcgggtaat gatcttacga gagatgtttt
      961 ccgcatcggc aagcgcagaa atatttcctg caaactcatt ctgcagccgg cttgtataac
     1021 gcaggccacg ttcataagca ctcgtcggcc gataatcgtt acccagtcgg gacaatgcag
     1081 ccatctgctc atcgtccagt tcaccaacca gaacgcgata atcactttcc gtcaggattg
     1141 cggctttacg gcgacggctg ccgtcggcaa tttctatgac atcagaaacc cggcggccga
     1201 aagctggagt ctgctgaccg gtcagtaaaa atgaaggaat gagatcatcc agagcatctt
     1261 cggtaagtag ttcctgatcg cgctcattgc ctgaccatac gcgtgaagcg ctctcaacag
     1321 agtctccccg gagcacctca agggtaaatt tcacttcccg tccacatacc ggaagagaga
     1381 ttgcattacc gcgggccata gcacccacgc gcgcaattaa tgaatccacc atcggcgcgg
     1441 ccggcgccgg tgatgaagta tcttcagtcg attgagtatg tgtggtatgt cttggaatga
     1501 caggagcacg cttcattatc taatctccca gcgtggttta atcagacgat caaaaatctc
     1561 attgcaaaca ggctcccaga tagagagggc attacgccag gcaccggttg aagaacgttg
     1621 atcaatagcc tgttcaaaaa cggttctcat acggatctga cctttaccga cttcatccgt
     1681 ttcgcgcaca acatttttaa ggaccatgct tccccaggca tcccgaattt gttcttccat
     1741 ccacggggac tgtgagccat tattattact gtatttggta agcagaatac ggacatctgg
     1801 ctcgaagcct ttaagatcca cgttcttaag caggtcacga agcatatcga aaaactgcag
     1861 tgcggaggtg tagtcgaaca gctctgccgg agtagggaca atcagaacat cagcagcgca
     1921 tacaacattt atcgtaccga taccaaggtt gggtgcactg tcgataacta tcacgtcata
     1981 atcatgagcg acagtttcaa tggccagacg gagcatcagg tgcgggtcag caggtaattt
     2041 gccttcgtca aatttcccca tcagctcggt ttcaatgcgg tgcagtgcca gacaggacgg
     2101 gatgatatca aggccaggcc agcaggtggg ctttatagcg tagctggcgt catccttttc
     2161 cccaagataa aagggcagga gagtgtcctc agcatgaata tgaagatcgg gcacccatcc
     2221 gtgatacata gaagcagttc cctgtgggtc attgccttca acaagcagaa cacggagtcc
     2281 tttcagagca agatcctgag ccaggtgaac ggatactgag gttttgtaaa caccaccttt
     2341 gtgagctgca acgccaatca ccggcggaaa cgcatcttca gcacggcgca gtcgtgtacc
     2401 aaatacatcc cgcatgtgat tgatttgttc gatggtataa ccaacgcgtt gctcaactct
     2461 cccacgggtt tccatatcag ggtgcggcag ccggccagct ttctcggcat ccctgatagc
     2521 ctgagatgac acgccaacta aatctgccgc ttcaccaatt ctccagcgcc gggttatttt
     2581 ccgggcttcc ggactgtcat cattaaactg tgcaatggcg attgctttcg tcatttcatg
     2641 accagcactt atgcactggt tgagtgtatc catgagtccc atactgaaca ccctttaatc
     2701 atcactttgc attattttat taaatcttgg agtttattgc aaagcaacaa taaaatcgca
     2761 aagcacacga aaaagtgcaa agtgaaccac attgggtaat gaaagaacgc aggggattac
     2821 aaaagagtac ataccgcagt caatattaat cactgccgct cgccgcagta agcgatctca
     2881 cacagtgaga aagcggcgag gaagcggaaa atcacagaac tcacagattg cacgtacgtc
     2941 gggttgctaa agatgactcg ggatcagcct gttataatga aattgactgc ggtatgtaat
     3001 atattgacta tagtatgatg cttaccgcat ctgtaggaaa gacgactcag cataccgcag
     3061 tcaatgtttc attgttgtac ctgtagatct caatcttcca gagcgtattc aggaaaatca
     3121 ggccagggtt ctggatatct ggtgtggcgc ttccggcctt atatctgggt tattcatggt
     3181 ctcgatccaa taattgatcc caaggagatc atagtgagat ccaaaagaga tccccggatc
     3241 gctccagcat gcgctgtaac tggctttaac gtctgtctgg atcgactgcg gaatgtaaaa
     3301 tgttgactgt tatatgcata atctaatact gtgatatgca acggtattta ctgaggtatg
     3361 tatatccgtt gactgcgata cgtattacta catccttttg tggataaccg ttgaggatca
     3421 agatgacgag cgaaaataat agcttacttc tgaaccttca ggaagttgat aagacaaccg
     3481 gtgaagttgt taagctggat gtcaacagca ccagtaccgt gcagccagta gcgctcatga
     3541 gactcggtct cttcgtccca actctgaaat caacagggaa gagcaaggcg aaccggaaaa
     3601 acgttacaga cgcgactgag gagcttgtac agctggccat tgccaaaagc gaaggataca
     3661 ctgacgttaa gatcaccggt tcgcgtcttg atatggacac ggattttaaa gtctggctgg
     3721 ggataattcg ctccatgtcg gagtatgggg taaaaagtga caccctggaa ctgtcgttcg
     3781 tcgaattcgt taagatgtgc ggatttgact cccgtcgttc aaacaaaaaa atgcgcgatc
     3841 gcatcagtaa ttccctgttt aaactcgcct cggttacgct gaagttccag agcgagacca
     3901 aaggatggac cacccatctg gtgcagtcag cctattatga catcaacgag gatatcgttg
     3961 agatcaaggc tgaacctaag ctgtttgagc tttaccacat ggacagaagg gtactgctgc
     4021 ggctgaaagc cattgacgcc ctgcagcgca aggaatcagc ccaggcgctt tatacctaca
     4081 tcgaaagtct tccccagaat cctgcaccta tttccatgaa acggatgcgt gaccggctga
     4141 atctcacctc aaatgtctat acgcagaacc acacggtgcg taaggcgatg gagcagctgc
     4201 gggatattgg ttatctggat tacaccgagt ttaaacgtgg ccgagccacc tatttcagcg
     4261 tgcattatcg caaccccaaa ctcatcagta gtcctgtaaa ggttccgcgc aaagaggaag
     4321 aagagaaggc gccagagcaa aattatgacg aagtcattaa ggcgctgaag gctgctggca
     4381 tcgatcctct taaactggct gaagccctct cagcgatgaa gccagaaaat tgactgcgat
     4441 atgcatatca tgtctgcccg gatcgaacaa tattgactgt catatgtgat ttcgggtcat
     4501 gatacgcaga tattgactac ggaatgcggg tttaatgggg gtaatccctg gtctgttcac
     4561 cattccatgg gggataacat gccacagtca atttttcagt aacgctctat atcccttacg
     4621 aaaatctcaa tcgggtgctg agacggtcat gttacctgac ggtatccgtt cccctgtgta
     4681 ctgcaggttt agcccggcag gatgagactg cagctgtctg tataacccgg cgccagcttc
     4741 acactgttaa atgcgatcag gaaacgggag ccgaatggca gaaaggtatt ggctagagat
     4801 ggtgaatgac tttcgccgtt aacgggatga tttgctgagc ttccaggaaa aagcgcaccg
     4861 tgggatgcac gggcagaatg cctgcatttt gccctgtctt gactgcggta tgttgatgcc
     4921 agcagaattg caggtcaccg ggtaagacca cggcgtttca gccatcagca tgatggcctg
     4981 gtgtgctgga gcagagagtg ggctgaatgc gctctgctcc tgtatttcgt attagcggaa
     5041 tacaggacgt catgccaggg ccgtcaatct tagccggccg ggggcaggga gcgaaggatc
     5101 tccgcggcat ctcgcccatc accggtaaag ggaacggcca gggtggcagc catgtccagc
     5161 gcaaataccc gggtatagac ttccatcgag cgcggatcct tatgcccggc cagtgcctgg
     5221 atgaccttcc tgggctgccg gtgataaagc atgtgcatga tatagctgtg ccggaaagta
     5281 tgcggcgtca cggggatcga aaaatgcacg ccgtcagctt cagcccgctt caccgcctgt
     5341 ttcagccagt tgcgcattgt ctcatccgtg accggccata acggctcccg ccggcggggg
     5401 cgggtggtca ccatccagct ttccatctga cgcacaaaac tggcatccgt cagcggcacc
     5461 agtcgcactt catcttttgg cggccgcccg cgccgcgcgc gcactttctc cgacagcacc
     5521 ctaacaaacg ggcgcagacc atccaggtcg aacgattccg gggtgagcgt ccgggcttca
     5581 ccgatacgga tcccggtatt ccacatggtg gcgaacaaca tgtggtgacg ctggtcaggc
     5641 atataccaga ggagggcgct cacttccggc gccaggaggt aagccggcgt gacacctgtt
     5701 gaggtggcca tccgccggag tgccaccgcg gtcgcaaaat caacgcccgg ggcgatcggc
     5761 agcagggaga cgcgttctgg tgaattctgc aggggaatga tgctgttcat gagttatttt
     5821 tcacgtagcg cacaacgggt ttggatacag ctattctggc ctgaagcggc tgatcctgcc
     5881 atgacaataa atcgcagcgc gcggatcctt ttgtttgctt atcccacatt atcgcgggta
     5941 aaaccaaatt ggaaaaggat tctttttttg ctcagatcct tcatgaagcc agtaatgaag
     6001 cgggtggagg gctgttttat cgtttttggg agaattgggg taatgccagg acaaacagga
     6061 tcgaaccaaa ggagagggag cagagaaggg gaggatgcgc tacgtgaatt ctgacctggt
     6121 acacccctgt aaccctgtca gtttcacgta gcacaatgtg gttgctgact actgccagtt
     6181 gagatgacgg ccggcatcct gatgctgact taatatatgg ataataataa tttcagtatc
     6241 agtctgcaga aaataaagca tatgtcattc aacggggagt gacaatatgt attccccagt
     6301 tcagggcggg aagtaccgat attatgatca cttaaaacaa aaaaaagccg gagagctgat
     6361 taatatatct gtcggccggt aatgactcca acttactgat agtgttttat gttcagataa
     6421 tgcccgatga ccttgtcatg cagctccacc gattttgaga acgacagtga ctttcgtccc
     6481 ggccttgcca gatgttgtct cagattcaga ttatgtcgct caatgcgctg agtgtaacgc
     6541 ttgctgataa cgtgcagctt tcccttcagg cgtgattcat acagcggcca gccatccgtc
     6601 atccatacca cgacctcaaa ggccgacagc aggctcagaa gacgctccag tgtggccaga
     6661 gtgcgttcac cgaagatgtg cgccacaact gtcctccgta tcctgtcata cgcgtaaaac
     6721 agccagcgct gacgtgattt agcaccgacg tagccccact gttcgtccat ttcagcgcag
     6781 acaatcacat cactgcccgg ttgtatgcgc gaggttaccg actgcggcct gagtttttta
     6841 agtgacgtaa aaccgtgttg aggccaacgc ccataatgcg tgcactggcg cgacatccga
     6901 cgccattcat ggccatatca ataattttct ggtgcgtacc gggctgagag gcggtgtaag
     6961 tgaactgtag ttgccatgtt ttacggcaat gagagcagag atagcgctga tgtccggcag
     7021 tgcttttgcc gttacgcacc acgccttcag tagcggagca ggaaggatat ctgatggaaa
     7081 tggaaaccac gcaagcacct taaaatcacc atcatacact aaatcagtaa gttggcagca
     7141 ttacccgcgc agtgagccca aatccgcatc tgaagcacgg tcaaaaaggt aatccagttt
     7201 gcgatttacg tcgctcagct ggttggtaac tatttttaac ccggactcca gatcacctgg
     7261 gccagcttca aggcagcgcc gcagatactc tgaccgatta ccacctgaaa ccaggtctat
     7321 ataggccaaa agttcatctg atacttttgc ggttattgtt ggcattcagt cctcactttg
     7381 tgcatttttt aaacgaaaaa agggtgtcta atacggtgga atcatagtat taccaaagta
     7441 ataaataaaa aattctttaa atcagtaagt tagattagat ttgtaatacc tgggtaatac
     7501 cgcagcatgg ccaaatttta tttttttatt ttaaatcagt atgatagcgt gatttatcac
     7561 gctgcgttag gtgtatagca ggttaaggga taaaaaatca tctttttttg gtagggcgat
     7621 ctccgtaggt taagggtcat ttgactaaaa agcgtcctat tctttgatgg tcatgcttgc
     7681 atgaccatct gagcaaccaa aactacaaaa aacgttttgc cttagtgttg gcagactaca
     7741 aataaactac aagaagacta caaataaact acaaaaaccg taaaagacta caaagagact
     7801 acaagaaaac tacagataaa ctacaaaact cgattgaccc cttcttacga gtgttgtaga
     7861 gtcattttca tacaacggag ggggttatga ataaaccagc agatctgaaa ccccgcaact
     7921 tatcggctgc tgtcagattg cgcctaaatg aaatcgagaa ctggctggac agagggctaa
     7981 cgcggcatga aattgctgaa atcctcgaca gcgaatacag cttttcggta acagccaaag
     8041 ggcttgagat ggcactgtat agaacgcgga aaaaccgaaa aaatgtattg tacaatacac
     8101 aaccgtctga gcctgaagcg caggaaagtg aaaaagcaga gagtcccggc attattgata
     8161 aagaattctt caataaaatc ggtgaggatt tcaaccctaa gaagttcaac aaaaaattct
     8221 gaggtgattt atgaaagtag cggtaattaa ttacagtggc agtgttggta aaaccttaat
     8281 ttcatcttac ctgttagccc cgcgcctgac tggtgcaaag ttctatgcgg tagagactat
     8341 caaccagtct gcttccgatc tgggtattga aaatgtgacc agttttaaag gtgacgactt
     8401 ctcacgtttg aggctttgtt gaataaatcg aacttttgct gagttgaagg atcagatcac
     8461 gcatcttccc gacaacgcag accgttccgt ggcaaagcaa aagttcaaaa tcaccaactg
     8521 gcccacctac aataaagccc tcatcaaccg tggctccata actttctggc tggatgatga
     8581 agccattcag gcctggtatg agtcgacaac gccttcatca cggggaagac ctcagcgcta
     8641 ttctgatctc accatcacca ccgttctggt cattaaacgc gtgttcaggt tgaccctgcg
     8701 ggctgcacag ggttttattg attccatttt tgcactgatg aatgttccgt tgcgctgccc
     8761 ggattacacc agtgtcagca agcgcgcaaa gtcggttaat gtcagtttca aaacgttcac
     8821 ccgaagtgaa atcgcgcatc tggtgattga ttccaccggg ctgaaggtct tcggtgaagg
     8881 cgagtggaaa gtcaaaaaac acggcaaaga acgccgtcgt atctggcgta agctgcatct
     8941 cgccgttgac agtaaaacac atgaaatcat ctgtgcagac ctgtcgctga acaatgtgac
     9001 ggactcagaa gccttcccgg gtcttatccg gcagactcac agaaaaatca gggcagcatc
     9061 ggcagacggc gcttacgaca cccggctctg tcacgatgaa ctgcggcgta agaaaatcag
     9121 cgcccttatc cctccccgaa aaggtgcggg ttactggccc ggtgaatatg cagaccgtaa
     9181 ccgtgcagtg gctaatcagc gaatgaccgg gagtaatgcg cggtggaaat ggacaacaga
     9241 ttacaaccgt cgctcgatag cggaaacggc gatgtaccgg gtaaaacagc tgttcggggg
     9301 ttcactgacg ctgcgtgact acgatggtca ggttgcggag gctatggccc tggtacgagc
     9361 gctgaacaaa atgacgaaag caggtatgcc tgaaagcgtg cgtattgcct gaaaacacaa
     9421 cccgctacgg gggagactta cccgaaatct gatttattca acaaagccga gtagtgctag
     9481 tgaggcgatg aataccgaca gttaacgtgc atgtgactta aacgtccgaa accgaacgat
     9541 tacgtacagc atgtagacaa catgtccgaa agtaagcaaa accattaacg gacagccgga
     9601 tcatttcgta catcccttac ggtcaggggt tcgcgctcaa tggaacgaaa tcacccgtta
     9661 gcttaaaacg cttaacgtag gataatttcc tttttccaag cggacccgat agacacacaa
     9721 agcccaatgg gtgaaatgta ctattccctc atcgctattt tttccaattt cgaggtagcc
     9781 agaaaccacg aaaggacaat ggccgggctt gcggccgcaa agaagaaagg acgcattgga
     9841 ggcagaaaac caaagctatc tccacaggac attaaagaaa ttaacgcgct tgttaaatcg
     9901 ccagaaatta cagtaaaaga aatcgcggcc cgttttaaag tttctcgacc tacgatttat
     9961 agacatattt catccgatat acaggatgaa taattaaccg ggcgctgccg gtatgggaat
    10021 caccccatgt tcgccgggcg ctgccggtgt gtcagctcat acagctatgc agctaatggc
    10081 ttgttgatga cactgaataa aaatttacca ttactatcaa tagagtaaga tgcttttaag
    10141 ggggtttttt cattgatttg ttttagcgct ggatctaaaa aagatcgtct caattcggct
    10201 aaatttttct gttgagactc aggtagcacg aaccgctcat tgagccaagc atgattggtt
    10261 atccaaagtc cagttgattt gaactgacag aggctttcgt agagtcttat tgtgcgcgga
    10321 tttcgtagac ttccacaatc atgcaatgaa taagtggtga actgcctggt aagtccataa
    10381 atgtacggaa gtagcttatg gttaaactct atttcccata ccccccttgt tgaacggttg
    10441 ccagcttcag taagccaggg tctggcaaca caatcatatc gaccttcttt tgggtaaaaa
    10501 acgactgaag aacggcttaa ttcaattatt ccatctttta catctttgac agcctgattc
    10561 cgtgtcacac cgaaaacggt tgcatattca gcaacggata ctttgaatac agcatctccc
    10621 aataccacat caggtttatc gctatcatct gtggatgaag cggtgaagta agtttgcata
    10681 agacacagcc acagaacgcg cttggccttc aaagagagat aatatgcggc ttcagtcaat
    10741 tcgttagcct gttttacatt aaccttttta ttcaatatct tatccatgtt atccaccttt
    10801 tacgggtgtt tttttatacc ccacaatacc acgattaggg atcttgtaaa ttatttttca
    10861 aaatcacatg aaaaggtggg ctgattccca taagaaggta ttatttattc ataaaaatca
    10921 atgtattacc tagtaataaa aggttgttaa aaagaaaata ttatataaat atttattaaa
    10981 aataaccctg gggataacta tctactcagc aaatgagcgt cattgctatc tgtcataaac
    11041 tcacatttgc gcttcctcac ttttgctgtt cgttcctcgc gttgctgcgg tacgtctcag
    11101 caaaagactg cggcgagcgc tgcgatctct taggtaagcg attaggtttg atgtagggat
    11161 ctagggggga taaaaagaat gaggcccatc accggcaagt aagtaagcgt aaactgaccg
    11221 ccgtatgtag ccatcagacg aaaattggta acttagacgc ccatctgata tagacggaca
    11281 tctaagtatg gaattacagg actggcgaaa agaactgaat cgccacaggt ttaatagaca
    11341 cctcagagtc atttaagatg acttaaagag aggtgcccat gagcggtaag cgttatcccg
    11401 aagagtttaa aattgaagca gtcaaacagg ttgttgatcg cggttattct gttgccagcg
    11461 ttgcaacacg tctcgatatc accacccaca gcctttactc ctggataaag aagtacggtc
    11521 cggattcttc cactaataaa gaacagtcag atgctcaggc cgagatccgc cgtctccaga
    11581 aagtgtagtg gtcaagtaat attggccacg gttttacagt aaaaatggta tctgttctct
    11641 gactcttccg gcgtcagccc accgttataa tggtgaggtc tgacgctgtt gtagtagttc
    11701 aatatgtaat cattaatttg ctgccgggcc tcgtccttgc ctgcgtaacc attcgttggc
    11761 acccattctg ttttcagact gcggaagaag cgttccatgg ggctgttatc ccagcagttt
    11821 ccccgccgac tgacgctttg atttattctg caacgccaga gaagttgttg atatttaagg
    11881 cctgtatact ggcttccctg gtcgctatgg aacacgacat cccggggttg gccacgcgtc
    11941 tcataggcca tccgcagggc actgcttatc agtgcggtat cggcatgcgc tgacagactc
    12001 cagccgataa ccctgcgggc aaaaagatcc ataacaaccg ccagatagca ccagcgattt
    12061 cctgcccaga gatacgtaat atctccacac catacccggt ctggctccgg tactgcgaac
    12121 tgacgctcaa gcagattcgg caggctggta tgctcctgac gggcattttt atactgatgt
    12181 tttccgggct gacaactgct caggttcaga tatttcatca gacgtccggc acggtaacgg
    12241 ctcatcggga cgccgttttg agtcagcatt tcagccagag tgcgcgcgcc cgcagagccc
    12301 cgactttggt tccacgcccg gcgtatttcg ctgcacaacc tgactcgtgc cggattaacc
    12361 gtatcgcgtc gttttcgcca gtaccggtaa ctgctgcggt gtatttccag agcagaacac
    12421 aggctgacaa ccgtgtggct gtcacttagt ctggcggcta tcgtgaaccg ttcagcgagt
    12481 cggacattaa gagcgcggta gcctttttta atatcgtatt ttgttcctcc agacggcgaa
    12541 cttgcttttc cagctcgcgg atacgttgct ggtctggagt aatgggtgtg gcagagggcg
    12601 taataccctg gcgctctcgc ctgagctggc gtacccagct ctcaagcgtg gttgaaccga
    12661 cattcatcgc ttcactgacc tgtcgatatg agtagccctt atcaacaatc agttgtgcac
    12721 attccagcct gaactcaggg gtgaaggttc gtttggtttt cttgttcatt aagtcacctg
    12781 ttttgtgttg tggtgagaat atcacctttc atcaggtggc caaatttagt gtgccactac
    12841 accctggaga gactatatgc cgcctcagtc agctcatttg actggacaat ccgcgggcta
    12901 tttttacgtt ttttatggtt tataaccgct atttccgcca tgacaggtcc atgtgaagtg
    12961 tgacagactt taagattgtc acacttcata aaacagagtc aacagggatg gataactttg
    13021 tgaaaagcaa cgcttcctga gggcaatttg tcacagggtt aagggcaact tgtcacagaa
    13081 agggcggcta tttgagggtg atttgtcaca ctgaaagggc gttttgtcac agatccctct
    13141 ctagaacccg catggataaa ggcctgtggg cattctaaaa ataaagatct aacaactatt
    13201 taaaaagatc ttataaaaat atccccgtgg ataagtggat aacccctagg ggaggtgatt
    13261 ttgcaggcat tgagcgtatg cagaatttat gagtactgtt ccatggtgct tcctcgctca
    13321 ctcggggctt cgccctgtcg ctcggctgcg gcgagcactg ctggctcgat tatatctgtg
    13381 gccagaagtt agacatacag cctattctgt ccagttctgg gtaatgctac caacttgctg
    13441 atttagtgta tgatagtggt tttgaggtgc tcccgtggct tccatctcca tcagttgtcc
    13501 ctcctgctca gctactgaag gcgtggtgcg taacggtaaa agtactgccg gacatcagcg
    13561 ctatctctgc tctcactgcc gtaaaacatg gcagctacag ttcacttaca ccgcttctca
    13621 acccggtacg catcagaaaa tcattgatat ggccatgaat ggcgttggat gccgggcaac
    13681 agcccgcatt atgggcgttg gcctcaacac gattttacgt cacttaaaaa actcaggccg
    13741 cagtcggtaa cctcgcgcat acagccgggc agtgacgtca tcgtctgcgc ggaaatggac
    13801 gaacagtggg gctatgtcgg ggctaaatcg cgccagcgct ggctgtttta cgcgtatgac
    13861 aggctccgga agacggttgt tgcgcacgta ttcggtgaac gcactatggc gacgctgggg
    13921 cgtcttatga gcctgctgtc accctttgac gtggtgatat ggatgacgga tggctggccg
    13981 ctgtatgaat cccgcctgaa gggaaagctg cacgtaatca gcaagcgata tacgcagcga
    14041 attgagcggc ataacctgaa tctgaggcag cacctggcac ggctgggacg gaagtcgctg
    14101 tcgttctcaa aatcggtgga gctgcatgac aaagtcatcg ggcattatct gaacataaaa
    14161 cactatcaat aagttagagt cattaccaca ttttgtgtca ataaaataat tatagaggct
    14221 cgggcaagca caaaatgctt gcccgatagg tcactctctt cattcacttt tgcaagagaa
    14281 ggaaacaatg cccggccata taacactatc tagcctaatt tggggtacca atacttatcg
    14341 aacaatttca tgtaatgcac gacgcgatag ttgtcgtttc acatatcgtt gcaaacggta
    14401 ggggataagt ttggttgccc aagataatgc aggattttgc cgcaacatcc ataatacagc
    14461 caataatttt tccttccttc catgaggttg agatattttt tcttcagcta ttgtgatgtt
    14521 ctctgaagaa tagaaactat ctatcaaagt tttaaaatca attctgccaa gctcgctttc
    14581 acgccaccat gaatcacgaa tataataatc ttgatagaaa tgatcaacac gtgtttctga
    14641 cgaaacattc ggcataggat caacattctg ttgctggaaa aagccatcga aatctgcaat
    14701 agcttgtcgc cataactcac tcattctttg gaaagatata tcccaattaa taatccggct
    14761 aaattttcgt ccgatgagtc gttcgccaaa ggcaccaata tttggtacaa caaccggtaa
    14821 cccatattga agtgctgcac tcaacgtata actatatgtc tcaggccatt gtgcagggaa
    14881 ccacattaca tccggagcaa tatcattgag ttttttctct aaatcctgat tagaatacgc
    14941 accatggcaa ataaccgcat catttagcgt actacaagcg tagccaagaa gatggaactc
    15001 aatgttttct cttttaagtg catgcgccac ctcattcagc atgaaagcgc ccttttcacg
    15061 actgatagcg ccaaggacaa ggactttcag tttccgatca cccgtgtaag ttttcctgac
    15121 atcaggatat ggagcatttt tttcgtaatc aggatgccat gcgatgacag ccttctcaga
    15181 aatatcggga aaatatcttg taaaccgaaa tttagtatct gccgacggga aaataactcg
    15241 ctcagcccca aataaccacg cattcatatt tgaacgccat gtggcagctg acattccaac
    15301 aggtaacgga tatgtttccg cgcataattc gtccatgttt gttgtattat cgccaataaa
    15361 aagaccattt ttgtcggtta gcgatgggtt tccatttatt gcaaagtagt catgaattgt
    15421 gacgtcatag gagcatttta aatctaaatg cagttttttg atcttctcag ggattcccat
    15481 caaatgatgg aaatggatat gcgatatacc caaaaagcgt aataatctca gcagatcatc
    15541 atagccattt tcaacatcaa cagagatact ttctttgcat gtgcgtccgt ctgcactcaa
    15601 agagatcact acgctactgt tgtctgcatt aggctttaaa ataaagaagc atgctcttcc
    15661 tttgtaaaaa ttagacaact cttcgagatg ataggttgtc ccgcccccca tatgatgaga
    15721 aaccagcagt attttctgaa gatttgcttt cgccagaatg cgcagtagca ttcgctggcg
    15781 agcttgctta gcaggatctc ttgaaataaa ctccattaca tcacgggtat agtttggatg
    15841 taattcatta aggagttcca tcgcgcgtat tttgcgcggg ctttgctcgc cagcgaaact
    15901 aactccgcct ttgtgataaa caaagacatt caattgatga aagttaggcc aaccggcttt
    15961 ggcagcacgt tgacaccagt cattttcctc accataacca cgaccaaaag tctcttcgtc
    16021 aaaatatcca acctggttta agcagtcacg acgaatgtac atacaaaaac caacacctgt
    16081 tggtatctcg acaagattat tttctttgcc ataagtttca aattcatcat cgagagcctg
    16141 gacatccatt ccggaaaata attcattatc ttcacagaaa tttgggaaac tacaaatcgt
    16201 cgcattattt gaaaatggag ttacgctccc tacacgctca cgactataag ctgcatcata
    16261 gatacgttcc agccatttat tagcgacttc aacatcgcta tttaagagaa gaacatcccg
    16321 tttcgaatgc agtttcatgc cacggttgac ggttgcaacg aaacctaaat tattttcgtt
    16381 ctcgagtaac ttgaaactat tgtcttgtgc acgtttacgt agccaatctg tcaattcagg
    16441 ttcaggtgaa gcatcattaa taacaattaa ttcagcccat gaggggaggg ttgatattgc
    16501 tgactctata cattcctgag tttcctgtaa accaccataa acgggtacga taacatcaac
    16561 caatatgtca ttattatcat tctcagcttg taacaatttc tcttgctgta actctaactc
    16621 tagtcggctc acaatatcat tcaactcaat attttttatc ttcaactctt cgagctcttt
    16681 caagagacct cgctcataca ttccattttc tacgacatct gtagtagatg ttttcatatc
    16741 aataatgctc tgtttttctc gtgacgatag ataatatagg atatgcttac ttaaaatttc
    16801 atgttcggat ggaaaggtca atcgaaagcg gatatattca gcgttaaata acaaatcaaa
    16861 tggaactaga taagaaaata aatagaaggt tagacgttca actcgctcat caatatcatc
    16921 aacattacaa aatgcataag aacaatcccc caaaatcttc acaggaatat tcattagcat
    16981 tgcttccaga ccgacactgg aattaatagt tagaattttt cgacatttct taagaaaatc
    17041 gatgattgac agattgttat tatcaatttc atatccaaga ttctcaatta atctaaaatg
    17101 gcttcctggg tgcggtcgta ctattttttt tccaataaat gaaccttcag catagtccaa
    17161 taaggcttga ttatcgaaac tattactaaa tgcaaggata ttggaatcgt tctcaacttg
    17221 caaaactata ccaacatcat attctggttc aatgtagtca tcatcgtttc gactgttaat
    17281 aaaaaaatca cgtaacgctg aaatgcgatt ttttttatta aagacaacac cttgttgttt
    17341 agcagcaaag tatctcttct ccgcttcagt gttgccattg acaccagaaa aatcgaaata
    17401 accagtaaaa agatacgtcg gagaacgcag cggacctaat tcaagattta taactggaat
    17461 attatatttt tctgctgcag cattcagtga tggacaattg catatggtca gaatacattc
    17521 aatgtcgttt tgtttttgta atgcagcgat tagagcattt ttaaaatcag gaatgatttc
    17581 cgtcagaaat ttcttaaaaa aggagattac tgttatcgcc acactcagtt aatgcttcag
    17641 ataatgattc ttcattaaca atatagacgt tgtctttttg gcgaactgaa aaattaggaa
    17701 gttcgtagcc taaacgtttc tgtccgtcat cagtaacctc ccagcgcccc tcttcctgcc
    17761 actttgtaca gggtaacaaa taatcttgtg tcgtaatcaa tgccaacggc tctttaatag
    17821 ccgtcatggc tttatataaa aaccacaaat aaggagcttt tacaccccgg aacggaaatg
    17881 cgggtaaata aatgccaatc atgatagttc atccccttct ataacattta caggagaatg
    17941 aataacacca ccaagttctc tggatgacca aaattttact tttgcaattg gacgtcgctg
    18001 atcaagctcc tcacgctcac cattattaaa acaagccaaa ccgcaatgaa cgagataatc
    18061 accagtattg ataggtagct taactttata tttaagcttc cgaagccccg gagccaagtc
    18121 aatcttatgc ccggcaaaaa tattattatc cccccagata tccccgcctt gggtccgaga
    18181 tagtgaaacg cctaagacta catcatttat attttctgat gagtttagct tgaaagaaaa
    18241 ataaatttct tcatttggtt ttacaagtgg cttttcagag agaggagtgc catctgcttt
    18301 cataattgag aaatcttcaa ttatagctcg accactacca aaccgttttt catcaacttc
    18361 ggcattggtt tgcgtatttt gtattgcgga taattcatca gcagatgcat tttcccccgt
    18421 ctgggaaaca gcttcttttt tctctgctgt atcagtgcgc tctgtcatca gcataccttt
    18481 ctcataggca agaacggcag ccaatacatc agtcgtattg ccgactaaca tgccatcact
    18541 cattacaagg cagcgatctg catattctaa tatgctacct gtggaatgcg tgaccataat
    18601 gatggttgtt cctttttgtt taagcatttc catgcgctta aagcacttat attgaaaacc
    18661 aatatcacct acagccaatg cttcatcaac aatcagtatt tcgggatcaa tacatgcctg
    18721 aacagcaaat gccagacgta catacatacc actggaatag gtctttaccg gttgatcaat
    18781 aaaatcacca atgtcagcaa aatctaatat ctcttgaaat ttcttatcaa tttccgactt
    18841 acttaaacct aatacgctac catttagata tacattatcg cggcccgaaa actctgggtt
    18901 aaaaccagct cccaactcaa gtaacgcagc cactcggccg ttaacttcaa tgtcaccgac
    18961 agttggagta agcgtaccgc aaatcatttg taataatgtc gacttcccag caccgtttcg
    19021 cccaatgata ccaacggttt cgccacgatt tatcgaaaat gaaacatctc gtagcgccca
    19081 aaactcttta aaatagctgc gactttcacg gcgaattaca ctttttattt ttggggcaaa
    19141 gaattgcttc aatctgtccc atggctggga atatatttga tagcatttgc ttaaattagt
    19201 tacctttatc gcgatttgat tagaggacat cagcaaatcc ttttcttgtt ttctggaaaa
    19261 acacaaagcc aagccaggcg acaatggatg ccccaacagt atagagcaaa atacccataa
    19321 aattaggtcc ccccccccaa atcaacacag tccgagcctg ctcaattata aatgtcaaag
    19381 gatttagcat gatccaaata tgatattttt caggtagcgc gctgataggg aaaaatactg
    19441 gcgataaaaa catcaaaacc gtcgttatga tgactgtagt ttggctaacg tctctcaaaa
    19501 atacgcctaa agaagccaaa atccatgata agccgagact gaaaataaca agaggcagaa
    19561 aaactaaagg aataaatatt attgtccagt gtataaaacc attaaatata acaaaagcgg
    19621 ttagtaaaac cagtagacta atagcagtat ggaataacgc agcaaccaag gatatgacag
    19681 ataacgtctc taaagggaat attacttttt tcacatagtt tgtattattt aatattatct
    19741 gtggtgccct attgattacc tcaccaaaaa aaccatggac gatcataccg acaaataata
    19801 ttattgcaaa ttgtgttctg ctttcatctc ctccagtcga ccatctggct ttaaatacca
    19861 ccgaaaaaac aaaggtatac acagtcaaca tgaataaagg attcaaaaat gaccataaaa
    19921 ctcccattac tgaaccttta tatctgccga taacatctcg cttagtcatt tgcaaaataa
    19981 tactacggtg agtatatata ctcttaacta tgtctaatgg agataatgac ggtttctgat
    20041 gtggattttt cattttaagc tctgaaataa taccaaataa attaacttag ctttcttggg
    20101 atattacgca atgctgattc gttgtaagca tatcgttttt tatcaaaaaa ccatccccac
    20161 ttattaaaat aggtaatggc cgaacgaata tgataccaaa gcaacaattt acttttataa
    20221 gatcccttat tgaaaacatg aacaattgtt gtttcggggc aataatagat cttcgtaagt
    20281 tgtaaagcac gacgacatag atctacatct tccaggtaca taaaatagcg ttcatcaaag
    20341 ccattcaact tttgtaatag aacattcgtc aaaagcataa aacagccaga taccgaagga
    20401 ggtgaaaata ccttatcata ctcggcatcc tttaactcgt atgtagcatc atacttaata
    20461 gcagtggtag gaagaaagcg tcttaagaag agattactgg gcgttggcaa caggcggcaa
    20521 ttatattgac gctcaccatt cttatagatg atctgcgggg atacaaaatt atattcattt
    20581 atccatgcaa acgatactaa tttatctaga gactgcttat caaaactgat atcaggatta
    20641 catattaaaa aatagccatc aaaatctgaa cttgctttga tggcgagatt atgtccggca
    20701 ccaaaaccac agttacgagg agacttaata taactcactt ttggttcatt caatgatgca
    20761 gcccactgac aaccaccatt atcaacgaag ataatttttt caacgctctc ttcatggcat
    20821 aacgagagaa gcgtatcttt aatatcatca taggaatgat taaataatac aatagaagca
    20881 ataacttttg gcttcattgc tttataagtc attaatcgat tacctttagg ataaattttt
    20941 aattattatt tttaaatagc cacttgtgat atccataatc taacaagcaa atttatactt
    21001 atgcgtagac ttcagctgtg gcaagagtat gtgcctgcag atctttctcc gacagcgaca
    21061 gcgcgcagtc gctcagtgac ggccagtcaa tccctatctc cggatcgttc cagatgatgc
    21121 ttcgctctga ctgtggcgcg tagtagttcg tcgccttgta gacaaactgc accgtgtcgc
    21181 tcagcgccat aaacccgtgg gcgaatcctt ccgggatcca cagctgacgt ttattctctg
    21241 cgctgagtac cgctcccacc catttgccaa aggttggcga tgaacgacgg atatccaccg
    21301 ccacgtcaaa tacctcacct tccacgcagc ggaccagttt gccctgagcg tgcggatcca
    21361 gctgatagtg cagcccgcgc aatacgcctt tctgggactg ggagtggttg tcctggacga
    21421 attccacctg gcggcccacc gcctcattaa acaccttgct gctgaagctc tcgaagaaga
    21481 agccccgcgc atcaccaaac acgcgcggtt caaaaatcat cacgtcaggg atctctgttt
    21541 taataatgtt catcgtttgc cacccgttta tttacccagg acttctgtta ccacgcgagc
    21601 cacaccctga cgccagtccg ggagagttac cccgaatgcc tgctggaatt tttcattcga
    21661 caggcgagag ttgagcggac gtttcgccgg cgtcggatag gccgtcgtcg ggatgccctt
    21721 cacttcctga accgccagct ctgcaccgtg cgctctcgcc acctcaaaca catagcgggc
    21781 gtagtcacac cagctggtct cgccgctggc caccagatga taggtaccgg ccagcgccgg
    21841 atcacgcagc gtttcacgga ttgccgtcgc cgtgcagtct gctagcagct ctgcgccggt
    21901 gggcgccccg tgctggtcgt caatgatgga gagcgtctct ttctcacccg ccaggcggag
    21961 catcgttttg gcaaagttgg cgccgcgggt ggcatacacc cagctggtac ggaaaatcag
    22021 gtgacgcggg ttgccctgct ccagcgccaa ttcaccggca cgtttggttt caccgtagac
    22081 gttaagcggg ccggttgcct catcttcccg gcgataatgg ctgccggcgc cgtcgaaaac
    22141 atagtccgtg gaatagtgca ccatcagggc acccagcttc gccgactccg tcgccagcac
    22201 cgccacccct ttgtcgttca gcagatcgga gagctcccgc tcgctttcgg ccttgtcaac
    22261 tgccgtgtgc gcggcggcat tcaccaccac gtccgggcgg acggtacgca cggtctgcgc
    22321 gataccgtcc agattagtca gatcgccgca cagctcttta tcaaagtaat ccaccgcgac
    22381 cacatcaccg agcgtggaca gtgaacgctg cagctcccag cccacctgac cattcttgcc
    22441 aatcagtaaa atcttcattc tctgtatcct gtcgttgacg aagaaggcat taacgctcgt
    22501 tcagcaggcg cttcagataa gcaccgtact cagttttctt caggcgttcg gcctgagccg
    22561 ccaccccttc cgccgacagc cactgattac ggtaggctat ctcttccaga caggcgacct
    22621 tcatcccctg gcgcttctca atggtatgaa taaactgaga agcttcaatc aggctgtcat
    22681 gggtgcccgt atccagccag gcaaatccgc gccccagcag ttccacctgc aaatcgccgc
    22741 gctccagata catctggttc agggtggtga tctccagctc gccgcgttca gaaggcttga
    22801 cgtccttcgc catctccacc acattgttgt cgtagaagta gagcccggtc accgcccagt
    22861 ccgatttcgg cttcagcggt ttctcctcga tggacagcgc cttaaagtta tcgtcgaact
    22921 ccaccacgcc aaagcgctcc ggatccagca cctgataacc aaacaccgtg gcaccggaag
    22981 tcttcgccgc cgcttccagt tttttaccga agctctggcc aaagtagata ttatccccca
    23041 gcaccagggc gcaggcatcg ttgccgataa acttctcgcc aatgataaag gcctgcgcca
    23101 ggccgtccgg cgacggctgg acggcgtagc tgaagttcac cccgaactgc gacccgtcac
    23161 ccagcaggcg ctggaaggaa ggcatgtcgt caggcgtgga gatgataagg atgtctcgaa
    23221 tacccgccag catcaggacg gacaccgggt agaaaatcat cggcttgtcg tagataggca
    23281 gcagctgctt ggagacgccc tgagtgatgg gatataaacg cgtgccggaa ccgccggcca
    23341 gaacaatacc tttcatcttt acttccttat tagttattca gaccaaggcg ctcaccggca
    23401 taggagccgt ccagcacgcg ctgccaccag gtctggttgt tcagatacca gtgcacggtt
    23461 ttttcaatcc cgctctcgaa cgtctcctgt ggtacccagc ccagatcgcg ctgaattttc
    23521 gcggcatcaa tagcgtagcg caggtcgtgt cccggacggt cggtgacaaa agtgatcagg
    23581 tcagcaaagt gggtgatgtt gcccggcttc tgcgccacca ccttgtcgag aatggcgcag
    23641 atggtgcgca ccacatcgat atttttacgc tcgttatgac ctccgatatt gtaggtctcg
    23701 ccgctcttgc cttcggtcgc cactttgtac agcgcacggg catggtcctc aacatacagc
    23761 cagtcacgga tctgctcccc gttgccatac accggcagcg gtttacccgc cagggcgttg
    23821 aggatagtca gcgggatcag tttctccgga aagtgatacg gaccatagtt gttggagcag
    23881 ttagtcacca ccaccggcag gccgtaggtg cggttccagg cgcgaaccag atggtcgctg
    23941 cccgctttag aagcagagta cgggctgctc ggcgcgtacg gggtttcttc ggtgaacagg
    24001 tcatcggtgc cgtgcaggtc gccgtacacc tcatcggtgg agatgtggtg gaagcggaag
    24061 gcctgcttct gcgcatcccc aaggccggac cagtagtgac gggccgcctc cagcagagtg
    24121 aaggtgccga tcacgttggt ctggatgaag gctgccgggc catcaatgga acggtccaca
    24181 tggctttctg ccgccaggtg catcacgata tccgggcgaa actcgctgaa ctgggccgcc
    24241 acggcagcag catcagtgat atcagtctgg gaaaacgagt agcgttcgct cccggccacc
    24301 ggcgccaggg attcgaggtt gccggcgtag gtcaggcagt ccagcacgcg aacttcatcc
    24361 cgggtatttt caatgatatg acggaccacg gcagagccga taaagcctgc accgccggtg
    24421 acaagaatct tcatactttc tctcgttttt caatgtttta attaatagtt tcagtactaa
    24481 taaaaaaacg caggcacgct accgccattg gctatcgcaa ccaaaatgcg tggtgcctca
    24541 gtaaattgta tttaacatga gctaagatca gagttccacg ctgtctcatc atggtacaaa
    24601 ccagcccaac cgcggtaact ttgcttgctt cagcgtacat tcgatatcag gactttccca
    24661 aacacaacaa tgtacgtcat cgagacgaaa tgtgaatgtg tctttaaagc acctgacaat
    24721 catcctttca aacgccattc agcgattggt atcatgacaa agccatacag tcaccattat
    24781 ggcgagcagc atactccaca gtgaatagta ccggacgagg tcgcgacgca ttatagcgct
    24841 ggtcatgaag aataaaaatc aaaaagcggt ttgaatcaat tcaaggtatg aatgaaacgt
    24901 tttcgataca atatcaaaaa attcaataaa ttgcccactg gattgccccc tatatttcca
    24961 tacacttttt atcacttaac ccattactgg ttcgccgccg cagatattcc cgtggcgaac
    25021 gataccccag tgcactatgc ggatgccatt cgttgtaatg ttcgaaggcc tccgcaaggt
    25081 tctttaccgc tgttaacccg tcgggtttcg gcatgatgct gatgtaatcg cgcttcatcg
    25141 ttttcacgaa gctctctgcc atcccgttgc tttccgggct acgtaccgcc gtatgtttag
    25201 gctccagtcc taccattctg gcgaactgac gcgtctgata agaacggtag gctgaaccgt
    25261 tgtctgtcag ccactcaact ggggatgtcg gcaggctgtt accgaaacga cgctccacgg
    25321 cacccagcat gacgtcctgc acggtttcac tgtcatatcc accgttactg gccgcccagt
    25381 aaagtgcctc gcgatcgcaa cagtccagag cgaacgtgac ccgcagtttt tcaccgttat
    25441 cacagctgaa ctcgaagccg tcagagcacc accgctggtt actttctcca acggtcactt
    25501 tccctgtatg cgcccgcttc gatggcggta tttccggttt acgctcaagc agcagcgcat
    25561 tctgacgcat gatgcggtat acgcgtttgg cattgatcac cgccatgtcg tcagtttctg
    25621 attgtctgcg cagcagtgcc catacccgac gataaccata ggtgggcaga tcgccgataa
    25681 cggtatggat acgggccagc gcgtcagtat catcaggctt gcgcttgcac cgacgatcct
    25741 gccagtcctt cgaccgacgg gccatggcat gcagttgcgc acgtgagacc cggaggcaac
    25801 gactgacaag gcttattcgc catcctccgg caacaagggc acgtgcgcta tccacttttt
    25861 ttgtcggcca tattcaacgg cttcttttag cagctcgttt tccatggttt tcttgcccaa
    25921 caggcgttgc agctctttaa tttgcttcat cgcagatgcc agctccgacg tgggcacaac
    25981 ctgttctcct gcggcaacgg ctgtaaggct gccttcctga tactgcttac gccacaggaa
    26041 cagctgactg gcagcaacgc catgctgacg ggcgaccagc gacacggtca ttccgggctc
    26101 aaaactctgc tgaacaatgg cgattttttc ctgaacactt cgccatctgc gcttctctgg
    26161 acctaaaaca tcaatcattc ggactccaac gactagtcta aaaactagta ttaagactat
    26221 cactaactta agtgatacca actgtctgga gattcagggg gccagtctac tgatcgcgta
    26281 ggccaacaga acgatgccgt taccggcatt atcatgaatc ccgttttttc agtaagttgc
    26341 agcatacaaa gtaattaaaa aaacgtcatt tcttgttgat gtatgcagta gcggtggtgc
    26401 gctacgtgaa atctggaaaa tgttggcggg gtaagtgact caatttttaa atctccgctc
    26461 ctgttttttt ggggatgtgt cagcaattat gacttggagc acaggtgtgt ccgacaacaa
    26521 aaattctgat caccaaaaaa aattttggac attttttcgg taatgactcc aacttactga
    26581 tagtgtttta tgttcagata atgcccgatg actttgtcat gcagctccac cgattttgag
    26641 aacgacagtg acttccgtcc cggccttgcc agatgttgtc tcagattcag attatgtcgc
    26701 tcaatgcgct gagtgtaacg cttgctgata acgtgcagct ttcccttcag gcgtgattca
    26761 tacagcggcc agccatccgt catccatacc acgacctcaa aggccgacag caggcccgga
    26821 agacgctcca gcgtggccaa cgagcgttca ccgaatacgt gcgccacaac cgtcctccgt
    26881 atcctgtcat acgcgtaaaa caaccagcgc tggcgtgatt tagcgccgac gtaaccccac
    26941 tgttcgtcca tttccgcgca aacaatgacg tcactgcccg gttgtatgcg tgagtttacc
    27001 gactgcggcc tgagtttttt aagtgtcgta aaatcgtgtt gaggccaacg cccataatgc
    27061 gtgcactggc gcgacatccg acgccattca tggccatatc aatgattttc tgatgcgtac
    27121 cgggttgaga agcggtgtaa gtgaactgta gctgccatgt tttacggcag tgagagcaga
    27181 gatagcgctg atgtccggca gtacttttac cgttacgcac cacgccttca gtagctgagc
    27241 aggagggaca actgatggag atggaagcca cgggagcacc tcaaaaacac catcgtacac
    27301 taaatcagta agttggcacc attacccggg aagaggggga aaatctagtg taacaaacta
    27361 ccctcaggat agggtgacaa attgccctca attctggctc cactaccggt tacagccaaa
    27421 aaaactatga gcaagcgcag gatgccaggg agggaacagc aaaactgtga caaatcactc
    27481 tcaaccttcc agatcaaatg tgacgaaccc cccttaaatc tgtgacagat aaccctcaac
    27541 tattcaatcg tcatggaggt tatatcacgg aaggaaaata cgatatgcgt cgtctggcgg
    27601 cctttctttt tctcgatgta agaaaggcgc attggtgtcc ggctgttgat ctcgtcaaca
    27661 cttgcctgca ggaaacggcg gcgaaagtcc ggcatacgct ggtaactttg aggtagctgg
    27721 tagcgttcca tgatccagtc gattttcagg gacacgacac ctgagccatc aggtttacgg
    27781 tactggcaca gagattcgta taaacgcatg gcgtacggat tggtaatctc ttttgtttca
    27841 ctgagccgga actgcgtaaa ccggttctgt aacccgatga agaagggaat cagatatggg
    27901 ttgatatgta cgctgtaaag ccctctggat gggctgtgcg cacgtttaat aaaccaggga
    27961 aaggattcat accctttttc atcgccggca tcctcttcag ggcgatagaa aaccacttcc
    28021 ttacccgcaa aaccttttaa agcctgtcgt atatccttac tggcttcaat cctcagagtc
    28081 cttggcagcg gggaacaaat gagaacacaa atgggctaat tcggcagtac tttcctaaaa
    28141 agacatgtct tgcccaatat actcaacatg aactagatct ggttgctgct cagctaaaca
    28201 acagaccgag aaagacactg aagttcaaaa caccgaaaga gataattgaa aggggtgttg
    28261 cattgacaga ttgaatctac agtagccttt tttaatatcg tattttgttc ctccaggcgg
    28321 cgaacctgct tttccagttc gcggatacgt tgctggtctg gagtaatagg tgtggcagag
    28381 ggcgcaatcc cctgacgctc tcgcctgagc tggcgcaccc aactctcaag cgtggtagaa
    28441 ccgacattca tcgcttcact ggcttgtcga tatgagtagc ccttatcaac aattagctgt
    28501 gcacattcca gcctgaattc aggggtgaaa gtacgtttgg ttttcttgtt cattaagtca
    28561 cctgttttgt gttgtggtga ggatatcacc tttaatcagg tggccaaatt tactgtgcca
    28621 ctacacaggc agataacctg accggcgata caggtgggac cgtggtccca ggcagataag
    28681 ctgacagacg atgcaggtgg gaccgtggtc ccagggagat aacctgaccg gcgatgcagg
    28741 tgggaccgtg gtcccaggca gataacctga ccggcgatac aggtaggacc gtggtcccag
    28801 gcagataacc tgacagacga tgcaggtggg accgtggtcc caggcagata acctgaccgg
    28861 cgatacaggt gggaccgtgg tcccaggcag ataagctgac cggcgataca gatgagaccg
    28921 tggtcccagg cagataacct gaccggcgat acaggtggga ccgtggtccc aggcagataa
    28981 gctgaccggc gatacagatg agaccgtggt cccaggcaga aaagctgacc ggcgatgcag
    29041 gtgggaccgt ggtcccaggc agataacctg accggcgata caggtgggac cgtggtccca
    29101 ggcagataag ctgaccgacg acgcaggtgg gaccgtggtc ccagggagat aacctgagcg
    29161 gcgatacaga tgggaccgtg gtccca
//
