LOCUS       NZ_CP063466             1563 bp    DNA     circular CON 17-JUN-2023
DEFINITION  Escherichia coli strain EM22-18-53 plasmid pEM22-18-53_7, complete
            sequence.
ACCESSION   NZ_CP063466
VERSION     NZ_CP063466.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN16387511
            Assembly: GCF_016942575.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1563)
  AUTHORS   Moser,A.I., Campos-Madueno,E.I. and Endimiani,A.
  TITLE     Complete genome and plasmid sequences of MDR Escherichia coli from
            local people and environmental sources in Laos
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1563)
  AUTHORS   Moser,A.I., Campos-Madueno,E.I. and Endimiani,A.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-OCT-2020) Institute for Infectious Diseases,
            University of Bern, Friedbuehlstrasse 51, Bern 3001, Switzerland
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Flye v. 2.7.1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 740x
            Sequencing Technology :: Illumina NovaSeq; Oxford Nanopore MiniION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 06/17/2023 06:38:10
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,984
            CDSs (total)                      :: 4,864
            Genes (coding)                    :: 4,564
            CDSs (with protein)               :: 4,564
            Genes (RNA)                       :: 120
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 300
            CDSs (without protein)            :: 300
            Pseudo Genes (ambiguous residues) :: 1 of 300
            Pseudo Genes (frameshifted)       :: 126 of 300
            Pseudo Genes (incomplete)         :: 171 of 300
            Pseudo Genes (internal stop)      :: 50 of 300
            Pseudo Genes (multiple problems)  :: 45 of 300
            Pseudo Genes (short protein)      :: 4 of 300
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP063466.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1563
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="EM22-18-53"
                     /isolation_source="chicken meat"
                     /db_xref="taxon:562"
                     /plasmid="pEM22-18-53_7"
                     /geo_loc_name="Laos"
                     /collection_date="2018"
                     /collected_by="Institute for Infectious Diseases Bern"
     gene            613..1092
                     /locus_tag="IQS26_RS24665"
                     /old_locus_tag="IQS26_24670"
     CDS             613..1092
                     /locus_tag="IQS26_RS24665"
                     /old_locus_tag="IQS26_24670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001620079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_000275450.1"
                     /translation="MYIGTEEQRKKRLKELEKLNKESESDPKNNGPFTQVYQKGWERIR
                     ELSKDKQGVVAIPLYSFLAEHIDPSCGAVVADQQFLADKLGVSRSTIKRWLNYLESKNA
                     LVRIPVAGKVCAYALDPHEVWKGYNTSKNYAAFVTKTLVNKDGDIQRRIMAMFSN"
                     /name="helix-turn-helix domain-containing protein"
     Typing          153..233
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="96.341"
                     /coverage="87.0"
                     /evalue="2.0600000000000002e-29"
     Typing          complement(1343..1562)
                     /name="Col(MG828)"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.57"
                     /coverage="84.0"
                     /evalue="1.12e-91"
ORIGIN
        1 aagaacaaca gcggaatcac cgacaacatg cgagaggtgg caccgctcgc cgcagcccca
       61 gcgaccgaac gttagtgagc gagtgggcga ggaagcggaa gagaaccgga aaccacattg
      121 agcacgtacg cactgatgcg gggtgtcggg gcgaagccct gaccagatgg caaacggaat
      181 atcgtcgcgg gtgacggtat tacatttgca catcctgtcc cgatttctga ggcgttttag
      241 cggtgaacgg acggcaaaac catgatggag ctgttgaacg ttgagagcgt gaaatctggc
      301 aaaaatcaca gctggtaagg cttgcagagg agtaagccag tatacactcc gctatcgcta
      361 cgtgactggg tcagggcttc gccccgacac cccaaaaaag tgttccgctt tgcgcaacac
      421 tttcagcaga aaagatattt aagggcgatt ttgtaagtta ttgatttttt agctacttgc
      481 attatgggtt agctcacgca tgtgtggtct aaggttagct cacgcatgtg tgagctaggt
      541 cgacctatca tggtttacgt ttgagctatg atggcttata tttgagccat cattagaccg
      601 tggagattta ccatgtatat cgggactgaa gagcagcgaa aaaagcgtct aaaagaactg
      661 gaaaaattaa acaaagaatc ggaatcagat ccgaaaaata acggcccatt cacccaggtt
      721 tatcagaagg gctgggaaag gatccgagaa ctgtcgaagg acaagcaggg agttgtagca
      781 ataccgcttt actcgttttt agctgagcac atagatccct catgcggtgc tgttgttgcc
      841 gatcagcagt ttttagcaga taaattgggt gttagccgaa gcacaattaa gcgctggctc
      901 aactacttag aatcaaaaaa cgcgttagtc agaattcctg ttgctggtaa ggtttgtgcg
      961 tatgcactcg atccacatga agtctggaag ggatacaaca catctaaaaa ctacgcggcg
     1021 tttgtcacta aaacactggt caacaaagac ggtgatattc agcgccgaat catggccatg
     1081 ttttcaaatt gagccagcgg caggcacagg caaaaacccc gtttttggac aatctgattc
     1141 aaatgttaac atttgacatc gggttgtcta tcctgcattg ctcttttgcc gcctcaaaat
     1201 cccttgcgtg tttttgctcc ccgatctcca gaacgatcga gccgccacgg ttccggcagc
     1261 gccttgagcg caaccggaaa ccgtctgggg agaaccccag acccccatcg ctgtaacacc
     1321 gacggtctac gaccacctac caaccgagtg gctgcctgta ccgtgcaaat gggctttgca
     1381 gggggctggc gcggttcctg gcgacctgaa agcccctggc taggggtatc ggcgcgcctg
     1441 acctcctgaa acgctctgta agcgtttttc gcgccttcca tgccctgacg ggcatttaaa
     1501 ccccgtttcg tgcgtctgag agcttctgag cgcgtttgaa gggctatctg acgacttatc
     1561 ccc
//
