LOCUS       NZ_CP063465             5809 bp    DNA     circular CON 17-JUN-2023
DEFINITION  Escherichia coli strain EM22-18-53 plasmid pEM22-18-53_6, complete
            sequence.
ACCESSION   NZ_CP063465
VERSION     NZ_CP063465.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN16387511
            Assembly: GCF_016942575.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5809)
  AUTHORS   Moser,A.I., Campos-Madueno,E.I. and Endimiani,A.
  TITLE     Complete genome and plasmid sequences of MDR Escherichia coli from
            local people and environmental sources in Laos
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5809)
  AUTHORS   Moser,A.I., Campos-Madueno,E.I. and Endimiani,A.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-OCT-2020) Institute for Infectious Diseases,
            University of Bern, Friedbuehlstrasse 51, Bern 3001, Switzerland
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Flye v. 2.7.1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 740x
            Sequencing Technology :: Illumina NovaSeq; Oxford Nanopore MiniION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 06/17/2023 06:38:10
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,984
            CDSs (total)                      :: 4,864
            Genes (coding)                    :: 4,564
            CDSs (with protein)               :: 4,564
            Genes (RNA)                       :: 120
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 300
            CDSs (without protein)            :: 300
            Pseudo Genes (ambiguous residues) :: 1 of 300
            Pseudo Genes (frameshifted)       :: 126 of 300
            Pseudo Genes (incomplete)         :: 171 of 300
            Pseudo Genes (internal stop)      :: 50 of 300
            Pseudo Genes (multiple problems)  :: 45 of 300
            Pseudo Genes (short protein)      :: 4 of 300
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP063465.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5809
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="EM22-18-53"
                     /isolation_source="chicken meat"
                     /db_xref="taxon:562"
                     /plasmid="pEM22-18-53_6"
                     /geo_loc_name="Laos"
                     /collection_date="2018"
                     /collected_by="Institute for Infectious Diseases Bern"
     gene            join(5157..5809,1..>4)
                     /gene="csa"
                     /locus_tag="IQS26_RS24605"
                     /old_locus_tag="IQS26_24610"
                     /pseudo=""
     CDS             join(5157..5809,1..>4)
                     /gene="csa"
                     /locus_tag="IQS26_RS24605"
                     /old_locus_tag="IQS26_24610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_104355618.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="pore-forming bacteriocin colicin S4"
                     /name="csa"
     gene            complement(join(<5809..<5809,1..86))
                     /locus_tag="IQS26_RS24610"
                     /old_locus_tag="IQS26_24615"
                     /pseudo=""
     CDS             complement(join(<5809..<5809,1..86))
                     /locus_tag="IQS26_RS24610"
                     /old_locus_tag="IQS26_24615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_104355619.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-A"
                     /name="colicin-A"
     gene            116..271
                     /gene="csl"
                     /locus_tag="IQS26_RS24615"
                     /old_locus_tag="IQS26_24620"
     CDS             116..271
                     /gene="csl"
                     /locus_tag="IQS26_RS24615"
                     /old_locus_tag="IQS26_24620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_071703583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin S4 system lysis protein"
                     /protein_id="WP_071703583.1"
                     /translation="MKKIIICAISLGIMLLAACQVNNVRDTGGGAVSPSSTVTGVSMGS
                     GGSGSL"
                     /name="csl"
     gene            complement(1269..1460)
                     /locus_tag="IQS26_RS24620"
                     /old_locus_tag="IQS26_24625"
     CDS             complement(1269..1460)
                     /locus_tag="IQS26_RS24620"
                     /old_locus_tag="IQS26_24625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_024192883.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDVDDQADICEALHDHA
                     DELYRSCLAHFGDDGENL"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            1528..1851
                     /locus_tag="IQS26_RS24625"
                     /old_locus_tag="IQS26_24630"
                     /pseudo=""
     CDS             1528..1851
                     /locus_tag="IQS26_RS24625"
                     /old_locus_tag="IQS26_24630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            1841..2152
                     /locus_tag="IQS26_RS24630"
                     /old_locus_tag="IQS26_24635"
     CDS             1841..2152
                     /locus_tag="IQS26_RS24630"
                     /old_locus_tag="IQS26_24635"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_205561675.1"
                     /translation="MIVKFHARGKGGGSGPVDYLLGRERNREGVRVLRGAPEEVRELID
                     ATPFAKKYTSGVLSFAEQTLPPGERERVMESFERVLMPGLEKKSVQHPVGGAPGQGAS"
                     /name="hypothetical protein"
     gene            2124..>2498
                     /locus_tag="IQS26_RS24635"
                     /old_locus_tag="IQS26_24640"
                     /pseudo=""
     CDS             2124..>2498
                     /locus_tag="IQS26_RS24635"
                     /old_locus_tag="IQS26_24640"
                     /inference="COORDINATES: protein motif:HMM:NF015397.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            2530..3090
                     /locus_tag="IQS26_RS24640"
                     /old_locus_tag="IQS26_24645"
     CDS             2530..3090
                     /locus_tag="IQS26_RS24640"
                     /old_locus_tag="IQS26_24645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000067489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_137561456.1"
                     /translation="MSNLLQMGTDFEKKLKERAVSTENMLNSEFRKLEASVDRELNLNG
                     QKIRDAISAHTAAVKEQLETLNSAVNAQFSAIENELARRQEELLWKLIKGRILYPSLTA
                     LCVTGGIFLASWGLIQWQESRIAANILAIRDQEETLAKLREKTWGVTYHEGRNGKFLVL
                     PSGVKGENNWTVEKKNAVRLVRE"
                     /name="MbeB family mobilization protein"
     gene            3097..3330
                     /locus_tag="IQS26_RS24645"
                     /old_locus_tag="IQS26_24650"
     CDS             3097..3330
                     /locus_tag="IQS26_RS24645"
                     /old_locus_tag="IQS26_24650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_053879377.1"
                     /translation="MTELEMHLLNALEQLQQDYTQRLKDWESAFTDLQHMFELTRQENW
                     QLREQCDDLSQQVQRLAGQVDRLSRLFITNSR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(3814..4236)
                     /locus_tag="IQS26_RS24650"
                     /old_locus_tag="IQS26_24655"
     CDS             complement(3814..4236)
                     /locus_tag="IQS26_RS24650"
                     /old_locus_tag="IQS26_24655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001255518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="WP_024216190.1"
                     /translation="MRSAATRLLLIPLITATIALTGCTPKTSLERHTRHYVYASDDGFD
                     PNFYTQKADTIRMMLPFFQQFRDMGMKDKAAGVSAETAQQRAKEFHSEKFFHSLQSTTT
                     FAGRKYTNSDMPSLKKMKLMADTISAVYLDGYEGRQ"
                     /name="Exc2 family lipoprotein"
     gene            4358..4636
                     /locus_tag="IQS26_RS24655"
                     /old_locus_tag="IQS26_24660"
     CDS             4358..4636
                     /locus_tag="IQS26_RS24655"
                     /old_locus_tag="IQS26_24660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000710672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_137561455.1"
                     /translation="MKKCVPVLLFSVLSASVASHADSNVFQQKAEKTKNGIVADVNARQ
                     KMFSNRLKIQLQIDELRAELAGTRDAKKREDLRKKLESLERQKKSLR"
                     /name="hypothetical protein"
     gene            complement(4628..4918)
                     /locus_tag="IQS26_RS24660"
                     /old_locus_tag="IQS26_24665"
     CDS             complement(4628..4918)
                     /locus_tag="IQS26_RS24660"
                     /old_locus_tag="IQS26_24665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001672407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_024216191.1"
                     /translation="MSKSPRHKLNKTDKRLLDTLVAAGYEHDKARDLIQKQVYTLTLAD
                     QRHVVSEISNGVNPTQAYSAVYQARRIRLARKYLNGKKVMEETGENTPPST"
                     /name="hypothetical protein"
     Typing          1097..1183
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="98.864"
                     /coverage="99.0"
                     /evalue="1.15e-36"
     Typing          complement(529..1073)
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="93.978"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          1421..1848
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="88.112"
                     /coverage="100.0"
                     /evalue="1.22e-76"
     Typing          2089..3437
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="98.444"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 ggtataactc cgattaccat aaataaatac aataagttat tggcttttct attgtctata
       61 tctattgaat gttcatcaat catcattgac ctataggatt ataataaagg tatatatgaa
      121 aaaaattatt atttgtgcca tttcactagg aataatgctg cttgcagcat gccaagtcaa
      181 caatgtcaga gatactggag gtggggctgt ttcaccatct tccactgtta ccggagtgag
      241 tatgggaagt ggtggtagtg gtagcctgta aaggatcttc ttgagatcct ttacagatcg
      301 tcgtagtctc ttgccctgta aacgaaaaaa ccgcctgggg aggcggttta gtcgaaggtt
      361 aagtcagttg gggactgctt aaccgggtag ctggcttaag tggagcacag ataccaaatg
      421 ctgtcctttc agtgtagccg ctataaggct tcaacttcaa gagttctgcg tatctaaatc
      481 cacttttcag ttaccaatgg ctgctgccag tggcgctttg tcgtgtctta ccggggtgga
      541 ctcgagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac
      601 acagcccagc ttggagcgaa cgacctacac cgaactgaga taccaacagc gtgagctatg
      661 agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt
      721 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc
      781 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg
      841 gagcctatgg aaaaacgcca gcgacgcggc tttttttgcc ctttgccttt ttgctcacat
      901 gttcttttct gccttatccc ctgattctgt ggataaccgt atcgccgttt ttgagcaagc
      961 agactccgct cgccgcagtc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga
     1021 aaagtgcctg gacgagcatt ttctccttac gcatctgtgc ggcatttcac acccggcatg
     1081 gtgcactttt catacaatcc tcactgatgc tacacgatta agccagtata cactccgcta
     1141 tcgctacgtg actgggtcat ggctgcgccc cgacacccgc taacacccgc tgacgcgccc
     1201 tgacgggctt gtctgctccc ggcatccgct cacaggccag ctgtgaccgt ctccgggagc
     1261 tgcatgtgtc agaggttttc accgtcatcc ccgaagtgtg cgaggcagct gcggtaaagc
     1321 tcgtcagcgt ggtcgtgaag cgcttcgcag atgtcagcct ggtcgtcgac gtccagttca
     1381 ttcagttttt ccagcagggt cagcgtctgg cttctgatga agcgggccat gttaagggcg
     1441 gttttttcct gtttagtcac agttctgttt ctccatgtgt ggggagtctg tcggaggttt
     1501 ttaatgatac cggtgaaacg cgaaaggatg ctcacgatac gggtcacgga tgaggaacat
     1561 gcgcggctgc tggcacgttg tgagggaaaa caacttgcgt catggatgcg taaggtctgc
     1621 ctgggggcac cgccatcgaa aacgtcaggt cttccgacac tggcaccgcc gctgttgcgc
     1681 cagttcgctt aggtcggcaa taacctgaat cagatagccc ggaagataaa cagcggacag
     1741 tggtcggggc atgaccgggt tcatgtggtg gctgcactga tggccatcgg acgtgaactg
     1801 agtgagctta gggatgaggt ccgtaaacag ggagaacggg atgatagtta agtttcatgc
     1861 caggggaaaa ggcggtggca gtggtccggt tgattacctg ctgggccggg agcgtaaccg
     1921 ggaaggagtg agagttctgc ggggtgctcc cgaagaggta cgggaactca tcgatgccac
     1981 gccgtttgcg aaaaaataca cctcgggggt tctgtctttt gcagagcaga ccctgccacc
     2041 gggagagcgt gaaagggtga tggagagctt tgagcgggta ctgatgcccg gtcttgaaaa
     2101 aaaatcagta cagcatcctg tgggtggagc accaggacaa ggggcgtctt gagctgaatt
     2161 ttgtcatccc gaatatggag ctggcgagcg gaaaacggct ccagccctat tacgaccggg
     2221 cggaccgtcc gcgaatcaat gcctggcaga cactggtgaa tcaccattac gggctgcacg
     2281 acccgaacgc accggagaac aggcggacac ttgtcacccc gaataatctg ccgaaagcga
     2341 aacaggaggc cgcagaagcg attacgaggg gactggaggc tctttaccat gccggagcgc
     2401 taaaaacgcg tcaggatgtc actgaggcgc ttacagcggc aggttttgag gttgtgagga
     2461 caaccaagag cagtatcagt atcgccgacc cggagggcgg gcgtaatctc aggctgaaag
     2521 gggcgctgta tgagcaatct tttacaaatg gggacagact tcgagaagaa actgaaagag
     2581 cgtgccgtct ctacagagaa catgctgaac agcgagttca ggaagctcga agcgtctgta
     2641 gacagggaac tgaacttaaa cgggcagaaa atcagagacg ctatcagcgc acacacagca
     2701 gcggtgaaag aacaactgga gacgctgaac agtgccgtga acgcgcagtt cagcgccata
     2761 gagaacgaac tggcccggcg gcaggaagag ctgctctgga agctgatcaa agggcggata
     2821 ctgtacccgt cgctgacggc gctttgcgtc acgggcggga tttttctggc gtcatggggg
     2881 ctgattcagt ggcaggaaag ccggattgcg gcgaacatcc tggctatcag ggaccaggaa
     2941 gagacgctgg cgaaactccg ggaaaaaaca tggggtgtga cgtatcacga gggcaggaac
     3001 gggaaatttc tggtgctgcc gtccggggtg aagggggaaa acaactggac ggtggagaag
     3061 aagaacgcag taagactggt cagggagtaa gtgagtatga cagagctgga aatgcactta
     3121 ctgaacgcct tagagcaatt gcagcaggac tatacgcagc ggctgaaaga ctgggagagc
     3181 gccttcacgg atttgcagca tatgttcgag ctgacacgac aggagaactg gcaactgagg
     3241 gaacagtgcg acgacttgag tcagcaggtg cagagattag ccgggcaggt ggaccgcttg
     3301 agtcggttat ttatcacgaa cagcagataa gggatgaaca ggaaaggcag gcacgcctgg
     3361 aacgggagcg tgcgcggtcc tggcatgaag agcaggaacg ggaagcacag gtatggcgtg
     3421 gcccgtcaat ggggatgtga cggtgagttg ttccgggagg ttgccagatg gtaccggaac
     3481 gcgttaaaaa cgcatccggg cgcgattctg gcgtgtcgtt tttgttgctg tgttttgcct
     3541 ctttgccaga tcgcgctcat tcctcagcct catcccgcga tgtctgcgta tgtcatcaac
     3601 tgaaaagatg gaaaaaaaac agtgtcatga ggtttacgca ggcaaaacca gcgttattca
     3661 catggctgaa tagctatgca tacggttaaa atttatcagg tgcgatcgcg gcagcttttc
     3721 gggtggtttg ttgccatttt tacctgtcag cagccgtgat cgcgctgaac gcatttcagc
     3781 ggtgcgtaca attaagggat tatggtaaat cccttactgt ctgccctcgt atccatcgag
     3841 ataaaccgca gaaatggtgt ctgccattag tttcattttt ttcagcgaag gcatatcgct
     3901 gtttgtgtat tttctgccag caaaggttgt tgtgctctgg agtgagtgaa aaaatttttc
     3961 tgagtggaat tcttttgcac gttgctgtgc cgtttctgct gatactccgg ctgctttgtc
     4021 tttcatcccc atatcccgga actgctgaaa gaacgggagc atcatacgta tggtgtctgc
     4081 tttctgggta tagaagttag gatcaaatcc atcatctgaa gcataaacat aatgtcgggt
     4141 atgtcgttcc aggctggtct ttggggtgca tcctgtaaga gctattgttg ctgttatcag
     4201 aggtatcaga agcagcctgg tagcggcaga tctcataaac atctccttgc gtttttaatc
     4261 ttgatactca gggagttata tctccgaaga gtatctctta tcatacgtcg gtttactccc
     4321 aatgtgattt gatggtcaat gagtcaggaa taatcccgtg aaaaaatgtg tgccagtcct
     4381 gctttttagt gttctttctg cttcggttgc cagtcatgcg gacagcaatg tttttcaaca
     4441 aaaggccgag aagacaaaaa acggcattgt tgcggatgtt aatgccagac agaaaatgtt
     4501 cagtaacaga ctgaagatac aacttcagat cgatgagttg agagcagagc tggctggcac
     4561 cagagatgcc aaaaaaagag aggatttgcg taaaaaactg gagtctctgg agcggcaaaa
     4621 gaaatcctta cgttgatggg ggcgtatttt ccccggtttc ttccataacc tttttaccgt
     4681 tcagatattt acgggcgagg cgaatgcgtc ttgcctggta taccgccgag taagcctggg
     4741 tgggattcac accattactg atttcactga ccacatgacg ctgatcagcc agtgtcagcg
     4801 tgtaaacctg tttctggatg aggtcacggg ctttgtcatg ctcatatccg gcagcaacaa
     4861 gggtgtcgag aagtcgtttg tctgttttgt tcagtttatg gcggggtgat ttgctcacat
     4921 cgttctccgt ttttcatgat ccaacagttt tatatctcat cctgatttgc tccggtgttc
     4981 tgtttcgacg ttctcaatgc ctgcctcccc atagcgtgat ccttatagca aaaatgaaaa
     5041 tccatgctct tgacatggac aatggtgaat agtaggtttt gctgtacata aaaccagtgg
     5101 ttatatgtac agtatactgt ttttaattta ttgttttaat tatcaaagag gaatttatgg
     5161 ctaaagaact aagtggatat ggcccaactg ctggtgagtc tatgggggga acaggggcga
     5221 atctgaatca gcaaggtgga aataataata gtaatagtgg ggttcactgg ggtggtggtt
     5281 caggctctgg taatggaggt cgtgaacatg gaagtcagac ggggtgggga tggagtaaaa
     5341 ccaataatcc ggatgtgccg ccttatgttg atgataatgg gcaggtgcgc attaccatca
     5401 caaatggtct ggtaaaaacg cctgtatatg gagttcctgg ggctggcgga aatagtgatg
     5461 ttcagggggg ctatatccct gaaaatccta atgatgaagt tgctcgtaag tgggataaaa
     5521 acaacttacc gcgagagatt gatgtgagta tcgatggttt taaatatcgt gtaacgctta
     5581 atgataatgg tagagctatc ggtattcttc gaacaggtgt ccgtccttac gtagggagtg
     5641 aaaaagctaa ggcgggtatt atggaaaaaa tcaatcataa aactcctgag gagatatatg
     5701 aggcgttagg ttttaataaa gatgaatctc agcggcagga aaaagcaaaa caacaggctg
     5761 aagatgcgtg ggatcgtttg cctcctaatg tgagaaaatt tgatgttga
//
