LOCUS       NZ_CP063460             2097 bp    DNA     circular CON 17-JUN-2023
DEFINITION  Escherichia coli strain EM22-18-53 plasmid pEM22-18-53_1, complete
            sequence.
ACCESSION   NZ_CP063460
VERSION     NZ_CP063460.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN16387511
            Assembly: GCF_016942575.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2097)
  AUTHORS   Moser,A.I., Campos-Madueno,E.I. and Endimiani,A.
  TITLE     Complete genome and plasmid sequences of MDR Escherichia coli from
            local people and environmental sources in Laos
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2097)
  AUTHORS   Moser,A.I., Campos-Madueno,E.I. and Endimiani,A.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-OCT-2020) Institute for Infectious Diseases,
            University of Bern, Friedbuehlstrasse 51, Bern 3001, Switzerland
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Flye v. 2.7.1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 740x
            Sequencing Technology :: Illumina NovaSeq; Oxford Nanopore MiniION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 06/17/2023 06:38:10
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,984
            CDSs (total)                      :: 4,864
            Genes (coding)                    :: 4,564
            CDSs (with protein)               :: 4,564
            Genes (RNA)                       :: 120
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 300
            CDSs (without protein)            :: 300
            Pseudo Genes (ambiguous residues) :: 1 of 300
            Pseudo Genes (frameshifted)       :: 126 of 300
            Pseudo Genes (incomplete)         :: 171 of 300
            Pseudo Genes (internal stop)      :: 50 of 300
            Pseudo Genes (multiple problems)  :: 45 of 300
            Pseudo Genes (short protein)      :: 4 of 300
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP063460.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2097
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="EM22-18-53"
                     /isolation_source="chicken meat"
                     /db_xref="taxon:562"
                     /plasmid="pEM22-18-53_1"
                     /geo_loc_name="Laos"
                     /collection_date="2018"
                     /collected_by="Institute for Infectious Diseases Bern"
     gene            complement(641..1630)
                     /locus_tag="IQS26_RS23145"
                     /old_locus_tag="IQS26_23155"
     CDS             complement(641..1630)
                     /locus_tag="IQS26_RS23145"
                     /old_locus_tag="IQS26_23155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001362017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="WP_205561672.1"
                     /translation="MKNPDLQNLTDYSPSDAPWDAHRSVSDDVGGIYLLAAEYERYGAR
                     MALCGGLLRFGWSTLKETGETRLRLREAHFCRVRHCPVCQWRRSLMWQARFYQSLPRIV
                     ADYPDARWMFLTLTVRNCAIGELGEMLNRMNAAFQRMKVRKEFRPVQGWIRTTEVTRGS
                     DGSAHPHFHTLMMVPPGMLNGKSYVRHERWVELWRECLRVDYDPNVDVRAVKPRKPKGG
                     ESLACATAELVRGAVAETLKYSTKPADMVADPEWFLELTRQTHKRRFVATGGALKDVLK
                     LDQETDADMVIGDDISEGDDDGSRIVFEWKTEVRKYRRSPTKDKTESG"
                     /name="protein rep"
     Typing          complement(558..639)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="93.976"
                     /coverage="90.0"
                     /evalue="3.4000000000000004e-27"
     Typing          724..915
                     /name="ColpVC"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="98.964"
                     /coverage="100.0"
                     /evalue="6.14e-93"
ORIGIN
        1 tcacaaaatc ggaacccacg ccggaggcgc gggcccccgc gattttctgt ttgacgaatc
       61 agggaacggt gaacggtacg cggaattttt tcgcgtaccg tgaggcgttg gcggggtgtc
      121 ggggcgcagt cctgaccagg ttcgtagaac cgcacagggt tgcaaagggg tgtccccttt
      181 ggcacaagcg acggagcgaa acgccccgtt ttgtgtacgc cgcttatcgt gcctgtgttt
      241 taggagaaca aaaaaatacc gtgcatacag cccacacaat gacccacata atcacccaca
      301 actcaaaaca cacagcccac acaatcaccc acactttaac ccacaaaaaa gaccacaacc
      361 ccattgacac cccacaaaac aacccacaca aacggcgagt aaatcatttt gccgttttcg
      421 tgccgcagcc gttccgcgca atcgacaaac tggcagtcag ggaatctcac gaagtgcgca
      481 cttatacaaa ctcaccttag gttcgtttgt cgtgcggtct gcgacgctca aaaaaccgag
      541 aaacggcggg aattttcggg acaggatgtg taattgtaat accgtcacac gcgacgctat
      601 cacaattgcc atctggtcag gggtgcaccc cgacaccccg ctagcctgat tcagttttat
      661 ctttcgtcgg tgagcgacgg tactttctga cctcggtttt ccactcgaac acgatgcgtg
      721 aaccgtcatc gtcgccctcg gaaatatcat caccgatcac catgtcggcg tcagtttcct
      781 gatcgagttt cagcacgtct ttgagtgcgc cgccggtcgc cacaaagcgg cgcttgtgcg
      841 tctggcgggt cagttcgagg aaccattcag gatcggccac catgtcagcc ggcttcgtgg
      901 aatatttcag ggtttcggcc accgcaccgc gcaccagttc ggcggtcgcg caggccagcg
      961 attcgccgcc cttcggctta cgaggtttca ctgcccggac atcaacattc gggtcataat
     1021 cgacccgcag acactcccgc cagagttcaa cccagcgttc atgcctgacg taacttttcc
     1081 catttagcat gccaggcggc accatcatca gggtgtggaa gtgcgggtgt gcgctgccgt
     1141 cgctcccgcg ggtgacttcc gtggtacgta tccagccctg cacaggccgg aactccttgc
     1201 gaaccttcat gcgctgaaaa gctgcattca tccggttcag catctcgccc agctccccga
     1261 tcgcacagtt gcgcacggtc agagtcagga acatccagcg ggcatcggga taatccgcca
     1321 cgatacgcgg cagggactgg tagaaacggg cttgccacat cagggagcgc ctccactggc
     1381 agacaggaca atgccggaca cggcagaaat gcgcctcacg cagcctcaga cgcgtttctc
     1441 cggtttcctt gagggtagac cacccgaaac gcagtaaacc accacagagg gccatacgcg
     1501 ccccgtagcg ctcatattcg gctgcaagca ggtaaatccc tcccacgtca tcagaaacgc
     1561 ttctgtgagc gtcccagggc gcatctgagg gggaatagtc cgtgaggttc tggagatcag
     1621 ggtttttcat gcgcaccacc cgatagtggg cgatggctca tctgacgtgt tatcattgcc
     1681 atatcacctg ttgtgttagt cgtatcacca attcgaattc ctacaggtgg tcacaccccc
     1741 gaacgattgc cctcgttgcg ggggtcgctt ttaatgcgac cactcccgac agaataactc
     1801 caaaaccatt ttccgacaaa tcccccctca ggccttgcgg cacaagggct ggaggcgaat
     1861 ttgaatccta gagttatccc ttaagtataa atacaaggat cgcgcgcgag atttgcttga
     1921 attttgagcg ggattttcag taacccaaaa ccacccaacc ccacccactt acacccatct
     1981 catccacaga ctttcacccc gattttgtga acaacaaaaa aaaccgtctt acaggtggtt
     2041 ggcctcactg cgtgacacca accaccatcc ccagtgtttt tcccgtgcct tcgtcaa
//
