LOCUS       NZ_CP063348             2028 bp    DNA     circular CON 17-JUN-2023
DEFINITION  Escherichia coli strain BS90R-A plasmid pBS90R-A_7, complete
            sequence.
ACCESSION   NZ_CP063348
VERSION     NZ_CP063348.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN16387358
            Assembly: GCF_016942505.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2028)
  AUTHORS   Moser,A.I., Campos-Madueno,E.I. and Endimiani,A.
  TITLE     Complete genome and plasmid sequences of MDR Escherichia coli
            acquired by Swiss travelers in Laos
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2028)
  AUTHORS   Moser,A.I., Campos-Madueno,E.I. and Endimiani,A.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-OCT-2020) Institute for Infectious Diseases,
            University of Bern, Friedbuehlstrasse 51, Bern 3001, Switzerland
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 344x
            Sequencing Technology :: Illumina NovaSeq; Oxford Nanopore MiniION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 06/17/2023 06:07:55
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,949
            CDSs (total)                      :: 4,830
            Genes (coding)                    :: 4,674
            CDSs (with protein)               :: 4,674
            Genes (RNA)                       :: 119
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 54 of 156
            Pseudo Genes (incomplete)         :: 103 of 156
            Pseudo Genes (internal stop)      :: 26 of 156
            Pseudo Genes (multiple problems)  :: 25 of 156
            Pseudo Genes (short protein)      :: 3 of 156
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP063348.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2028
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="BS90R-A"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pBS90R-A_7"
                     /geo_loc_name="Switzerland"
                     /collection_date="2018"
                     /collected_by="Institute for Infectious Diseases Bern"
     gene            1..441
                     /locus_tag="IN877_RS24480"
                     /old_locus_tag="IN877_24480"
     CDS             1..441
                     /locus_tag="IN877_RS24480"
                     /old_locus_tag="IN877_24480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000031238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="WP_024192735.1"
                     /translation="MSKDTQRITQINDQGEIVGGFVAVIRPKQKSSFQRHFTMNQDALK
                     ILAKELTGEQFKVLMLMLADLDYENFIQIAQADIADALEMQKTHVSRAVRALLDVGVIF
                     EGPKVGRSKTYRLNEQFGWKGTVSNHKKALKNGLSVIQGGRT"
                     /name="MarR family transcriptional regulator"
     gene            complement(505..1473)
                     /gene="mobV"
                     /locus_tag="IN877_RS24485"
                     /old_locus_tag="IN877_24485"
     CDS             complement(505..1473)
                     /gene="mobV"
                     /locus_tag="IN877_RS24485"
                     /old_locus_tag="IN877_24485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000024818.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobV family relaxase"
                     /protein_id="WP_000024818.1"
                     /translation="MANYAIMRCKKLTGMGSVASALQHCYRERETPNANAERTPENYCS
                     VSKSTDQAMGRVRELLPEKRRKDAVLAVEYVMTASPEWWKEATPRQQAEFFARSEQWLE
                     KKYGKDRVVAAVVHRDEATPHLSAFVVPLTQDGRLSAKEFIGGRSKMREDQSTYAESVK
                     KLGLERGIEGSRATHQTVQHYYESINRGTRSQVSISPEALEPRVLRKGIFTKDVEDQAA
                     IAKRLSHAVNDGFAGTIAMASQSAQNAKRARELQKTMDAQQKRLQSVTEPFKGLSREQM
                     TEILMMAQRFKQQNQEKEKQQRIEREKQRQTRSRGMGGMER"
                     /name="mobV"
     Typing          complement(509..1482)
                     /name="MOBV"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="95.077"
                     /coverage="96.0"
                     /evalue="0.0"
ORIGIN
        1 atgagcaagg atacccagag aatcacccag attaacgatc aaggtgagat tgttggcggc
       61 ttcgtcgcgg taatcagacc caagcaaaaa tcgtcatttc agaggcattt cactatgaac
      121 caagacgcat taaagatcct cgctaaggag ctaacgggcg agcaatttaa agttctaatg
      181 ctcatgctcg ctgatctgga ttacgagaat ttcatccaaa ttgcacaggc tgatatagcg
      241 gatgcattgg agatgcaaaa gacccacgta agccgcgctg ttcgtgctct tcttgatgta
      301 ggcgtgatct ttgaaggtcc gaaagtaggt agatccaaaa cctatcggtt gaacgagcaa
      361 ttcggctgga aaggaacagt aagcaatcac aaaaaagccc ttaaaaacgg cctcagcgtt
      421 atacagggcg gcagaacctg atctctggaa tgccgcaaat gacgtcactt tgcggcgtta
      481 gccctatcct gcatcgcatc aaaactaacg ctccatacca cccatgccac gcgatcgcgt
      541 ctgtcgctgt ttttcacgtt caatacgctg ctgtttttcc ttttcctggt tttgctgctt
      601 aaaccgctga gccatcatca ggatttcggt catctgctct ctggaaagcc ccttaaaagg
      661 ctctgtaacg ctctgtaggc gtttctgctg cgcatccata gtcttctgta gctcacgcgc
      721 tctttttgcg ttctgggcgc tctgagaggc catagcgatg gttcctgcaa agccatcgtt
      781 cactgcgtgg cttagacgtt tggctatggc tgcctgatct tccacgtctt tggtgaaaat
      841 gcccttccgc aatacacgcg gctccagcgc ctccggcgag atcgatacct ggctacgggt
      901 gccacggttt atcgattcgt agtagtgctg aactgtctga tgcgtggcgc ggcttccctc
      961 gatgccgcgc tcaagaccca gtttttttac tgattcagcg taggtgctct gatcttcacg
     1021 catctttgac cgcccgccaa taaactcttt cgcggacagc cgtccatcct gcgtcaatgg
     1081 caccacgaac gcagacaggt gcggggtggc ctcgtcccgg tgaaccaccg ccgccacaac
     1141 acgatccttc ccgtatttct tctccagcca ttgctcagag cgggcgaaaa actccgcctg
     1201 ctgccgtggt gtggcctctt tccaccactc agggcttgct gtcataacgt attcaacggc
     1261 cagtaccgca tctttacggc gtttttccgg cagcagctcc cgcacacgcc ccattgcctg
     1321 atccgttgat ttggacacag agcagtagtt ttctggtgtc cgttcagcgt tagcgttcgg
     1381 cgtctcacgc tctcggtagc agtgctgcaa cgcgctggcc acgcttccca ttcccgtcag
     1441 ctttttgcag cgcattatcg cgtagttcgc catgttcccc cctgattccg gcattcattc
     1501 agccaggcgg gggagcgcat tgcgatacct ccggcgggcc actatcaaga tgatagtgta
     1561 ctcactagac tttgcttcgc aaaatcgttc gggcagcaag ctgccagaag gtcaaaatca
     1621 acggcggtct ggcaggtgga aggcgtcgct ccgcgccacc cccaccactg ccaagcctgt
     1681 tttatccgga gtgtcgtcaa gggtaaatgg cactcgcttc gctcgccctt gacgaaatca
     1741 ggcaatgaat atcgcttttg aaagagcagg aaccgctcgc cgcagactca tgaccgaacg
     1801 tagtgagcga atgagcgagg aagcggaatt tccactcccc gtacatagaa atccgtacac
     1861 tccgtagcta aaaaacgttg gtattcagag ctaaaccgcg cgcacgcgtt ataagataca
     1921 gaaggagcga taaaaaaacg tatgtatggc gaggggttac atttgacata gttaccaata
     1981 atggtaacac tgttaccaat aatggtaact caagataggt tattttcg
//
