LOCUS       NZ_CP063344            27893 bp    DNA     circular CON 17-JUN-2023
DEFINITION  Escherichia coli strain BS90R-A plasmid pBS90R-A_3, complete
            sequence.
ACCESSION   NZ_CP063344
VERSION     NZ_CP063344.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN16387358
            Assembly: GCF_016942505.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 27893)
  AUTHORS   Moser,A.I., Campos-Madueno,E.I. and Endimiani,A.
  TITLE     Complete genome and plasmid sequences of MDR Escherichia coli
            acquired by Swiss travelers in Laos
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 27893)
  AUTHORS   Moser,A.I., Campos-Madueno,E.I. and Endimiani,A.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-OCT-2020) Institute for Infectious Diseases,
            University of Bern, Friedbuehlstrasse 51, Bern 3001, Switzerland
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 344x
            Sequencing Technology :: Illumina NovaSeq; Oxford Nanopore MiniION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 06/17/2023 06:07:55
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,949
            CDSs (total)                      :: 4,830
            Genes (coding)                    :: 4,674
            CDSs (with protein)               :: 4,674
            Genes (RNA)                       :: 119
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 54 of 156
            Pseudo Genes (incomplete)         :: 103 of 156
            Pseudo Genes (internal stop)      :: 26 of 156
            Pseudo Genes (multiple problems)  :: 25 of 156
            Pseudo Genes (short protein)      :: 3 of 156
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP063344.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..27893
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="BS90R-A"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pBS90R-A_3"
                     /geo_loc_name="Switzerland"
                     /collection_date="2018"
                     /collected_by="Institute for Infectious Diseases Bern"
     gene            join(27607..27893,1..691)
                     /locus_tag="IN877_RS24235"
                     /old_locus_tag="IN877_24235"
     CDS             join(27607..27893,1..691)
                     /locus_tag="IN877_RS24235"
                     /old_locus_tag="IN877_24235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6685 family protein"
                     /protein_id="WP_024245156.1"
                     /translation="MRYLTELICDLYRLTDHHKLTTATFKNLADIKLFKPTADADTILQ
                     LENIFSEYSLRYIDKDLAEILSNLITTDKKHSLDFDFNKIQSLTDSKSFGCGWSKVING
                     SWFKDLYSWGEGMYPAENLKAENILDWKDNIWHIEHEGFPPRPPINVKYYSWFDRYVAS
                     NSGGSHHAAMVVYQSVRDGLEYKREAVIKQLSIDPGTVEILDKNYYSFIFQIKTLNKET
                     RIYTSEYEFTDALKDFGQNRHTILKPVNYVSNIKLVFIPKDALKTNSETFRNWFHSAIS
                     CGKIISLPDYLKNPAHYHTLHYSHEVDSITLGDPSRKFKLKGDS"
                     /name="DUF6685 family protein"
     gene            complement(688..1044)
                     /gene="ddp1"
                     /locus_tag="IN877_RS24240"
                     /old_locus_tag="IN877_24240"
     CDS             complement(688..1044)
                     /gene="ddp1"
                     /locus_tag="IN877_RS24240"
                     /old_locus_tag="IN877_24240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000202958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA distortion polypeptide 3"
                     /protein_id="WP_000456533.1"
                     /translation="MEYQVREFINEKYTKAVNILKDNLKENYHVFYGVRLIEILFPASE
                     YGTDAFFKEFELINSVILPLVIFDLTQRKPMMIISFDKILDASLLEGTNIVVLECITLA
                     DLLTNDNIDFLYKS"
                     /name="ddp1"
     gene            complement(1181..1627)
                     /locus_tag="IN877_RS24245"
                     /old_locus_tag="IN877_24245"
                     /pseudo=""
     CDS             complement(1181..1627)
                     /locus_tag="IN877_RS24245"
                     /old_locus_tag="IN877_24245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001074384.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /name="hypothetical protein"
     gene            complement(1667..2503)
                     /locus_tag="IN877_RS24250"
                     /old_locus_tag="IN877_24250"
     CDS             complement(1667..2503)
                     /locus_tag="IN877_RS24250"
                     /old_locus_tag="IN877_24250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001050932.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="WP_001050931.1"
                     /translation="MNLLSVTKKTKIRHRNEINSTFSSLPLSARRILFMAMAQIDSREE
                     LSEGATFRITAREYAFIADIDVTGAYRQLKEGAEELQASVIRIPRNQLLTPQGNQSSGH
                     TKKRGKLPSDAVRLMNLTAFCDYVESEGYIDIAFSHVIEPYITMLKDSYTTQVLISSVR
                     LADQNSNMLYQFIRKQYSSGKKTFFDIEVDVLKDELELFRIDNGEKVYLYQNFKDFNKV
                     FLQKNIEKINQYTEINHLEVKIVERVARRASKLRFSYKIDKESEGLDIRIPYGFRG"
                     /name="RepB family plasmid replication initiator protein"
     gene            3737..3988
                     /locus_tag="IN877_RS24255"
                     /old_locus_tag="IN877_24255"
     CDS             3737..3988
                     /locus_tag="IN877_RS24255"
                     /old_locus_tag="IN877_24255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000121741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid stabilization protein"
                     /protein_id="WP_000121743.1"
                     /translation="MSYQILTTTAASITDLKKNPMGTVAEGEGDAVAILNRNEPAFYCV
                     PPKLYAYYRELAEDAELNAVADERMKNPEIVKVNLDDL"
                     /name="plasmid stabilization protein"
     gene            3978..4259
                     /locus_tag="IN877_RS24260"
                     /old_locus_tag="IN877_24260"
     CDS             3978..4259
                     /locus_tag="IN877_RS24260"
                     /old_locus_tag="IN877_24260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001694627.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_000220560.1"
                     /translation="MTYELAFDRRALKEWQKLGHTIREQFKKKLAERLENPRVPAARLH
                     GHADRYKIKLRASGYRLVYQVIDEKVVLLVIAVGRRESSEVYQIADLR"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            4334..5031
                     /locus_tag="IN877_RS24265"
                     /old_locus_tag="IN877_24265"
     CDS             join(4334..4583,4583..5031)
                     /locus_tag="IN877_RS24265"
                     /old_locus_tag="IN877_24265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743396.1"
                     /ribosomal_slippage=""
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1-like element IS1A family transposase"
                     /protein_id="WP_103215986.1"
                     /translation="MASVSISCPSCSATDGVVRNGKSTAGHQRYLCSHCRKTWQLQFTY
                     TASQPGTHQKIIDMAMNGVGCRATARIMGVGLNTILRHFKKLRPQSVTSRIQPGSDVIV
                     CAEMDEQWGYVGAKSRQRWLFYAYDRLRKTVVAHVFGERTMATLGRLMSLLSPFDVVIW
                     MTDGWPLYESRLKGKLHVISKRYTQRIERHNLNLRQHLARLGRKSLSFSKSVELHDKVI
                     GHYLNIKHYQ"
                     /name="IS1-like element IS1A family transposase"
     gene            5072..5386
                     /locus_tag="IN877_RS24270"
                     /old_locus_tag="IN877_24270"
     CDS             5072..5386
                     /locus_tag="IN877_RS24270"
                     /old_locus_tag="IN877_24270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001749965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrbM/KikA/MpfK family conjugal transfer protein"
                     /protein_id="WP_000734776.1"
                     /translation="MKKLLIPLIAAGSLLYLPASHAEDPCKVIMCMAGKLTGDSGGSEC
                     NSAEAAFFNIVKKNKHGFLPNHTKDARKAFLNECPDNGEGGSNQSMISQIISKYGKVRL
                     "
                     /name="TrbM/KikA/MpfK family conjugal transfer protein"
     gene            5422..5733
                     /locus_tag="IN877_RS24275"
                     /old_locus_tag="IN877_24275"
     CDS             5422..5733
                     /locus_tag="IN877_RS24275"
                     /old_locus_tag="IN877_24275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001452736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001452736.1"
                     /translation="MKKIILSALACTALLTGCVSQDKGNAMQSQLNNQQRQINELSVRL
                     QSAESRLSKQEEKLRNELLQSSGYCYLNGARYSTGTVLYGRICQNQSGSASWQVYSRR"
                     /name="hypothetical protein"
     gene            5921..6424
                     /locus_tag="IN877_RS24280"
                     /old_locus_tag="IN877_24280"
     CDS             5921..6424
                     /locus_tag="IN877_RS24280"
                     /old_locus_tag="IN877_24280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000893479.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0004519 - endonuclease activity [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="WP_241088267.1"
                     /translation="MLERIKTLPGFPQKINYLRKIDPFVFEELLLEGFEAHGFRTIRNK
                     RYTGDGGIDGQVIIGKYRYLIQAKRYRGHIALQHVQEFEKLLKRHNCRGLFCHTGKTGA
                     GSKSVSIASERMEIISGQRLIDLLTPGSSFTIATAPQTMMKRTAATLETSTIVKDAGVM
                     TPTY"
                     /name="restriction endonuclease"
     gene            complement(6418..7115)
                     /locus_tag="IN877_RS24285"
                     /old_locus_tag="IN877_24285"
     CDS             complement(join(6418..6866,6866..7115))
                     /locus_tag="IN877_RS24285"
                     /old_locus_tag="IN877_24285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743396.1"
                     /ribosomal_slippage=""
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1-like element IS1A family transposase"
                     /protein_id="WP_103215986.1"
                     /translation="MASVSISCPSCSATDGVVRNGKSTAGHQRYLCSHCRKTWQLQFTY
                     TASQPGTHQKIIDMAMNGVGCRATARIMGVGLNTILRHFKKLRPQSVTSRIQPGSDVIV
                     CAEMDEQWGYVGAKSRQRWLFYAYDRLRKTVVAHVFGERTMATLGRLMSLLSPFDVVIW
                     MTDGWPLYESRLKGKLHVISKRYTQRIERHNLNLRQHLARLGRKSLSFSKSVELHDKVI
                     GHYLNIKHYQ"
                     /name="IS1-like element IS1A family transposase"
     gene            complement(7149..7742)
                     /locus_tag="IN877_RS24290"
                     /old_locus_tag="IN877_24290"
     CDS             complement(7149..7742)
                     /locus_tag="IN877_RS24290"
                     /old_locus_tag="IN877_24290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold-containig protein"
                     /protein_id="WP_000599943.1"
                     /translation="MILFADYNTPYLFAISFVLLIGLLEIFALICGHMLSGALDAHLDH
                     YDSITTGHISQALLYLNIGRLPALVVLCLLAGFFGLIGILLQHACIMVWQSPLSNLFVV
                     PVSLLFTIIAVHYTGKIVAPWIPRDHSSAITEEEYIGSMALITGHQATSGNPCEGKLTD
                     QFGQIHYLLLEPEEGKIFTKGDKVLIIWVMLPTY"
                     /name="OB-fold-containig protein"
     gene            8011..8211
                     /gene="glgS"
                     /locus_tag="IN877_RS24295"
                     /old_locus_tag="IN877_24295"
     CDS             8011..8211
                     /gene="glgS"
                     /locus_tag="IN877_RS24295"
                     /old_locus_tag="IN877_24295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface composition regulator GlgS"
                     /protein_id="WP_046788569.1"
                     /translation="MDNSLNSLNNFDFLARSFARMHAEGRPVDILAVTGNMDEEHRTWF
                     CARYAWYCQQMMQARELELEH"
                     /name="glgS"
     gene            complement(8254..9318)
                     /locus_tag="IN877_RS24300"
                     /old_locus_tag="IN877_24300"
     CDS             complement(8254..9318)
                     /locus_tag="IN877_RS24300"
                     /old_locus_tag="IN877_24300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="WP_001269817.1"
                     /translation="MRYLLTVIAFIMGFSALPVWAMNCYAEHEGGNTVVIGYVPRIAIP
                     SDGKKGDKIWQSSEYFMNVFCNNALPAPSPGEEYPSAWVNIMMLLASGQDFYNQNSYTL
                     GVTYNGVDYDSTSTRAIAAPECIDVKGAGIYSNTYKNPAVCSGGPEPQLSVTFPVRVQL
                     YIKLAKNANKVNKKLVLPDEYIALEFKGMSGTGTIDTDKNLTFRIRGLNNIHVLDCFVN
                     VDLEPADGVVDFGKINSRTIKNTSVSETFSVVMTKDPGAACTEQFNILGSFFTTDILSD
                     YSHLDIGNGLLLKIFHNDGTATEFNRFSQFASFSSSSAPSVTAPFRAELSANPAETVVE
                     GPFSKDVILKITYN"
                     /name="fimbrial protein"
     gene            complement(9320..10069)
                     /locus_tag="IN877_RS24305"
                     /old_locus_tag="IN877_24305"
     CDS             complement(9320..10069)
                     /locus_tag="IN877_RS24305"
                     /old_locus_tag="IN877_24305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417519.4"
                     /GO_process="GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="WP_001352149.1"
                     /translation="MRYLNTKNIIAAGVLLSCMNSIAWGAIIPDRTRIIMNESDKGEAL
                     KLTNQSKKLPYLAQTWIEDTKGNKSRDFIVTVPPMVRLNPNEQIQIRMITQEKIAQLPK
                     DRETLFYFNVREIPPKTDKKNVMQVTMQHALKLFWRPKAIELEDDGVMTYEKVEIIRRN
                     DGSIRFNNKMPYHVTLGYIGTNGVTMLPQTQSLMVLPFSHADTQFKNVPSNFQVGYIND
                     FGGLSFYEINCPTVNNSCNVSVAKRDK"
                     /name="molecular chaperone"
     gene            complement(10091..>11842)
                     /locus_tag="IN877_RS24310"
                     /old_locus_tag="IN877_24310"
                     /pseudo=""
     CDS             complement(10091..>11842)
                     /locus_tag="IN877_RS24310"
                     /old_locus_tag="IN877_24310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001480735.1"
                     /GO_function="GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA]"
                     /GO_process="GO:0009297 - pilus assembly [Evidence IEA]"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /name="fimbria/pilus outer membrane usher protein"
     gene            complement(12004..>12465)
                     /locus_tag="IN877_RS24315"
                     /old_locus_tag="IN877_24315"
                     /pseudo=""
     CDS             complement(12004..>12465)
                     /locus_tag="IN877_RS24315"
                     /old_locus_tag="IN877_24315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221000.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS26 family transposase"
                     /name="IS6-like element IS26 family transposase"
     gene            complement(12550..14121)
                     /locus_tag="IN877_RS24320"
                     /old_locus_tag="IN877_24320"
     CDS             complement(12550..14121)
                     /locus_tag="IN877_RS24320"
                     /old_locus_tag="IN877_24320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017782005.1"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="WP_039026336.1"
                     /translation="MTHIACHINTLQLPFVACTVPLTFTLPAAGCTLIGRNGIGKSLLL
                     ELLAGQRRADSGKIEWLTPISWLSQFAPAQDHDCAASVADLIGWGEPLRALERIEQGSC
                     AQDDFELLADRWNLRSDLADLLAEAGLRLDPHVPVSTLSGGQLTRLRLLALFARPAHFL
                     LLDEPDNHLDASGIDWLSQQLARHRGGYLLVSHDRRLLNQTSHILELSEQGLQSARLSF
                     DEFLAQQAEVQAARSTSLAQAVRSEKAAARSLQKVREQAARRASQGRRERGSQAKILLD
                     FKAEQAGKSLGRVLDRHERGHSAQVEARTEAAAALAAVQPLIMQWPEEIERKGAAQPLV
                     ITAASHVRWHGQRVDLVLQRGERCLLGGRNGCGKSSLLQMLRGVLEPETGSFRINGKMA
                     CLDQGLSLIARDLSPLANLLTVAPTLAESDARTRLAGIALRGDRALTPCHGLSGGERLK
                     LGLLMVLAQLPDLLLLDEPDNHLDHPSRQLLTQVLADYPGTLLLVSHDPDFVAGVGVGK
                     ELNLSE"
                     /name="ATP-binding cassette domain-containing protein"
     gene            14548..15108
                     /locus_tag="IN877_RS24325"
                     /old_locus_tag="IN877_24325"
     CDS             14548..15108
                     /locus_tag="IN877_RS24325"
                     /old_locus_tag="IN877_24325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004636773.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0000150 - DNA strand exchange activity [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="WP_039022047.1"
                     /translation="MQGQRIGYIRVSSFDQNPERQLEHIQVDKVFIDKASGKDTQRPQL
                     DVLLSFVREGDTVVVHSMDRLARNLDDLRRLVQGLTQRRVCIEFVKEHLTFTGEDSPMA
                     NLMLSVMGAFAEFERALIRERQREGIALAKQRGAYRGRKKSLSDPEIAEVRRRVIAGEK
                     KATLAREFGISRETLYQYLRVPE"
                     /name="recombinase family protein"
     gene            15112..18144
                     /locus_tag="IN877_RS24330"
                     /old_locus_tag="IN877_24330"
     CDS             15112..18144
                     /locus_tag="IN877_RS24330"
                     /old_locus_tag="IN877_24330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006087332.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
                     /protein_id="WP_039022048.1"
                     /translation="MAIAKSERLTVLSDAEQEALYGLPDFDDAQRLEYLALTENELALA
                     SSRPSIHAQVYCILQIGYFKAKNDFFRFYWRDVEDDCAFVLSRYFQGEAFETKQISKHE
                     HYSQRGKIAALFGYQPWASSYLPSLEHQASLIVRRDVTPNFVAAELIVWLNEQKIIRPG
                     YTTLQALVSEVLSAERRRLGALLAQVLDEPAKTSLSQLLVRDDTLSQLAALKQDAKDFG
                     WRQMARERDKRTLLAPLHGIAKGLLPKLGISQQNLLYYASLANFYTVHDLRNLKPEQTQ
                     LYLLCYAWVRYRQLSDNLVDAMAFHMHQLDHENRAGARESFAEEQVKRHQETPQVGRLL
                     SLYVDDSVADPTPFGEVRQRAYKIMAKDVLQNTAQRMSVKPLNKLTLHWQAVDGQAKRI
                     SRHLRPLFVALEFAATDPDSQWLAALTWVKSVFAKQLRLSQRPLAECPAATLPKRLRPY
                     LLSFDADGEPTGLHADRYEFWLYRQVTKRFQSGELYLDDSLQHRHFSDELVSMEEHAQE
                     LAQMDIPFLRQPVETQLDALASELHKQWRAFNRELKQGKLTHLEYDKDTQKLTWRKPKG
                     ENQKAREQTFYDQLPYCDVADVFRFVNNQCQFLSALTPLQPRYAKKVADTDSLIAVIIA
                     QAMNHGNLVMARTSDIPYHVLESAYQQYLRQASLHEANDGISNAIAALPIFPYYSFDLD
                     VLYGAVDGQKFSVERPTVKARYSRKYFGRGKGVVAYTLLCNHIPLNGYLIGAHDYEAHH
                     VFDIWYRNTSDIVPEVITGDMHSINKANFAILHWFGRRFEPRYTDLNAQLQALYCVGDP
                     ARYEKCLIQPVGQIDRQLIVSEKANIDRIIATLGLKEMTQGTLIRKLCTYTAPNPTRRA
                     IFEFDKLVRSIYTLRYLRDPQLERNVHRSQNRIESYHQLRSSIAQVGGKKELTGRTDIE
                     IEICNQCARLIANAVIYYNSAILSRLLTKCEATGNAKALAALTQISPAAWRHILLNGHY
                     TFQSDGKVIDLDALVAGVELA"
                     /name="Tn3 family transposase"
     gene            complement(<18176..18424)
                     /locus_tag="IN877_RS24335"
                     /old_locus_tag="IN877_24335"
                     /pseudo=""
     CDS             complement(<18176..18424)
                     /locus_tag="IN877_RS24335"
                     /old_locus_tag="IN877_24335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221000.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /name="IS6 family transposase"
     gene            18475..18705
                     /locus_tag="IN877_RS24340"
                     /old_locus_tag="IN877_24340"
     CDS             18475..18705
                     /locus_tag="IN877_RS24340"
                     /old_locus_tag="IN877_24340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000844627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_038989484.1"
                     /translation="MQQCPRERQAHEAGARTRATAHERTPQQERQKAAREAERGREAWT
                     LGQGMKKPVAGCYGRLTRWKGGGDVVYMALL"
                     /name="hypothetical protein"
     gene            complement(18737..19387)
                     /gene="tetR(A)"
                     /locus_tag="IN877_RS24345"
                     /old_locus_tag="IN877_24345"
     CDS             complement(18737..19387)
                     /gene="tetR(A)"
                     /locus_tag="IN877_RS24345"
                     /old_locus_tag="IN877_24345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000164043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance transcriptional repressor
                     TetR(A)"
                     /protein_id="WP_001493764.1"
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWHF
                     RNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAGTR
                     PGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGGTVE
                     QAPLSPLLRAAIDAFDEADPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
                     /name="tetR(A)"
     gene            19493..20692
                     /gene="tet(A)"
                     /locus_tag="IN877_RS24350"
                     /old_locus_tag="IN877_24350"
     ARG             19494..20689
                     /name="tet(A)"
                     /gene="tet(A)"
                     /gene_name="tetracycline efflux MFS transporter Tet(A)"
                     /tool="amrfinderplus"
                     /drug_class="TETRACYCLINE"
                     /identity="99.75"
                     /coverage="100.0"
     gene            complement(20724..21608)
                     /locus_tag="IN877_RS24355"
                     /old_locus_tag="IN877_24355"
     CDS             complement(20724..21608)
                     /locus_tag="IN877_RS24355"
                     /old_locus_tag="IN877_24355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000058717.1"
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="WP_000058717.1"
                     /translation="MSLRTPDLLFTAIAPAIWGSTYIVTTQYLPNFSPMTVAMLRALPA
                     GLLLVMIVRQIPTGIWWMRIFILGALNISLFWSLLFISVYRLPGGVAATVGAVQPLMVV
                     FISAALLGSPIRLMAVLGAICGTAGVALLVLTPNAALDPVGVAAGLAGAVSMAFGTVLT
                     RKWQPPVPLLTFTAWQLAAGGLLLVPVALVFDPPIPMPTGTNVLGLAWLGLIGAGLTYF
                     LWFRGISRLEPTVVSLLGFLSPGTAVLLGWLFLDQTLSALQIIGVLLVIGSIWLGQRSN
                     RTPRARIACRKSP"
                     /name="EamA family transporter"
     gene            complement(21746..22138)
                     /locus_tag="IN877_RS24360"
                     /old_locus_tag="IN877_24360"
     CDS             complement(21746..22138)
                     /locus_tag="IN877_RS24360"
                     /old_locus_tag="IN877_24360"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013053.2"
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family cysteine hydrolase"
                     /protein_id="WP_001351729.1"
                     /translation="MLFPNFSLGEEYEHAPPATNRQISPYLPSGRFRTGLPVEGLAIER
                     GDLFYACPRASVFYGTALDADLRTRGVSTLVMAGISTTGVVLSSVAWASDADYDVRLVQ
                     DCCYDPDRDAHEALLRSGFGGRVQVV"
                     /name="isochorismatase family cysteine hydrolase"
     gene            <22142..23893
                     /locus_tag="IN877_RS24365"
                     /old_locus_tag="IN877_24365"
                     /pseudo=""
     CDS             <22142..23893
                     /locus_tag="IN877_RS24365"
                     /old_locus_tag="IN877_24365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001138014.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3-like element TnAs1 family transposase"
                     /name="Tn3-like element TnAs1 family transposase"
     gene            <23927..24495
                     /locus_tag="IN877_RS24370"
                     /old_locus_tag="IN877_24370"
                     /pseudo=""
     CDS             <23927..24495
                     /locus_tag="IN877_RS24370"
                     /old_locus_tag="IN877_24370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743396.1"
                     /note="frameshifted; incomplete; partial in the middle of a
                     contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IS1-like element IS1B family transposase"
                     /name="IS1-like element IS1B family transposase"
     gene            <24511..24849
                     /locus_tag="IN877_RS24375"
                     /old_locus_tag="IN877_24375"
                     /pseudo=""
     CDS             <24511..24849
                     /locus_tag="IN877_RS24375"
                     /old_locus_tag="IN877_24375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016241261.1"
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]; GO:0043565 - sequence-specific DNA
                     binding [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(24863..25297)
                     /locus_tag="IN877_RS24380"
                     /old_locus_tag="IN877_24380"
     CDS             complement(24863..25297)
                     /locus_tag="IN877_RS24380"
                     /old_locus_tag="IN877_24380"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_053273090.1"
                     /translation="MIALSIIRLLHYMEQTPDTYSYTACHIGCSSVLVEDSSTPARATN
                     SGLENTKKPDYQSGFFVPVVRLFFRFFPSFGGFFFHFLLRPILLFALANCVMALLTFKS
                     IVRNNTVQNIYIRACTEVSLTINEHLDQNLLAEIIGKIRK"
                     /name="hypothetical protein"
     gene            25278..25361
                     /gene="dinQ"
                     /locus_tag="IN877_RS24385"
                     /old_locus_tag="IN877_24385"
     CDS             25278..25361
                     /gene="dinQ"
                     /locus_tag="IN877_RS24385"
                     /old_locus_tag="IN877_24385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible type I toxin DinQ"
                     /protein_id="WP_000565612.1"
                     /translation="MIDKAIIVLGALISLLELIRYLLQLMG"
                     /name="dinQ"
     gene            complement(25516..26160)
                     /locus_tag="IN877_RS24390"
                     /old_locus_tag="IN877_24390"
     CDS             complement(25516..26160)
                     /locus_tag="IN877_RS24390"
                     /old_locus_tag="IN877_24390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001503708.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0000150 - DNA strand exchange activity [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="WP_000203396.1"
                     /translation="MTSNVPTSFVRAYLRASTAEQDASRALETIEAFARERGLIICSYY
                     IENESGTRLERPELFRLLRDCQQNDILLIEDVDRLSRLSGEDWNTLKKMIRQKDIRVMA
                     VNVPTTWINSGMSEFDSRLFAAINDMLLDMLAAVARRDYEQRRERQKQGIEKAKKDGKY
                     KGRKPNQARHDAIIRLIESGSSWTQVQKVLGCSRGTISNALKRKTGQKSSK"
                     /name="recombinase family protein"
     gene            26541..27203
                     /locus_tag="IN877_RS24395"
                     /old_locus_tag="IN877_24395"
     CDS             26541..27203
                     /locus_tag="IN877_RS24395"
                     /old_locus_tag="IN877_24395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000516401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division plane positioning ATPase MipZ"
                     /protein_id="WP_000516402.1"
                     /translation="MGKILLVVSDKGGVGKSTYVANTGSMLVNKGKSVIILKTDKNHDL
                     LSWNEKRTDNGLLTIPVHAAYGNVSNEIKRLSKLCEVLIVDCPGHDSQEFRSALTVSDI
                     VVTLVKPSSDFESETLTSVTEKIRTAQKANAALEPWVLFTRINSAKPRHRKAAIDLDKL
                     LRSDNIWIQPLKTRISELDVYESACNEGAGVHDVSRASSLSTAKAQIELVAQEIGIL"
                     /name="division plane positioning ATPase MipZ"
     gene            27301..27582
                     /locus_tag="IN877_RS24400"
                     /old_locus_tag="IN877_24400"
     CDS             27301..27582
                     /locus_tag="IN877_RS24400"
                     /old_locus_tag="IN877_24400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001185482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001185482.1"
                     /translation="MALKLNRPNIDESQQPATNAETARFISGATRAPVVGKPKLMNFRL
                     SAHFEDILEAEAIRTGQSKTTVLKAALAMYNSQDENIKNHWLLESAKF"
                     /name="hypothetical protein"
     Typing          complement(1182..1627)
                     /name="IncX1"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="98.21"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 tggatggagc aaggtaataa atggtagttg gttcaaagat ctttattctt ggggagaggg
       61 gatgtatcct gccgaaaatc tgaaagctga aaatatttta gactggaaag ataatatatg
      121 gcacattgag catgaaggtt tccccccacg cccccctatt aatgttaaat attattcttg
      181 gtttgaccga tatgtagctt caaacagtgg tggctcacat catgcagcaa tggttgtata
      241 tcaatcagtc agagacgggt tagagtataa aagagaagct gttattaaac aactgagtat
      301 agacccaggc actgttgaaa tacttgataa gaattactat tcattcatat tccagatcaa
      361 aacccttaat aaagaaacta gaatttatac atctgaatac gagtttacag atgctttaaa
      421 agactttgga caaaacagac ataccatact taaacctgtt aattacgttt ccaacataaa
      481 attagtattt attccaaaag atgcattaaa aacaaatagc gagacattcc gcaactggtt
      541 tcattctgca attagctgcg gaaaaataat atcactccct gattatctga aaaatccagc
      601 acattatcat acacttcatt actcacatga agtagactca ataacacttg gcgacccaag
      661 cagaaaattt aaactgaaag gagattctta gcttttatat aaaaaatcaa tgttatcatt
      721 tgtcagaaga tcagcgaggg taatacattc aagaaccaca atatttgttc cttccaacaa
      781 actggcatca agtatcttat cgaagctgat aatcatcatt ggcttgcgtt gtgttaaatc
      841 aaagattacc aatggaagta ttacactgtt aattaattca aactctttaa agaaagcatc
      901 agtgccatac tcactggctg gaaaaagaat ctcaattaat ctcacaccat aaaaaacatg
      961 gtagttttct ttaaggttat cctttaaaat attaacagcc ttagtatatt tttcgttaat
     1021 aaactctctc acctgatatt ccataaaatt ctccgacaag aaccgactgt aggtcaccgg
     1081 gcaaacgttg cagaatggcg tcagagacgt cattttgcgg cgtttgccct atcctgcatc
     1141 gcagtgaaaa ggcagggact ggcataactg ctgagtagca tcattgctgg cttatctcgc
     1201 tactgttctc ggtgctttcg tccgcaggtt cgggaacaag taacttgtgc caaggcatag
     1261 ttttgttgag cagctccatc agaggctcag ccagcagtcc caaaatccag gttgccgtaa
     1321 tcaccggtat agtcagattt tgtggcgtcc ataactggtg atcgagatga taccaagtga
     1381 tgacacacaa aataccaccc agccagaata cagcccattt aaacgcacca agaatggcta
     1441 acacaaaaac aaagataaaa tgtaaaacac tcgcgacaag aagacgaaga taaaaccatc
     1501 ctcagccaag ccacttaaca aaagtgctcc ctgttttcac tgcaaaacgt ttcttacgct
     1561 ccctggcaat ttctttcctg aatttagcac gttcttcccc ctgagggaat tgatagattt
     1621 tagccatgag ctacctcatt tcgatctcaa aacgaagttt atgcaattat cctctgaaac
     1681 cgtatggtat tctgatatct aagccctcac tttctttatc tattttataa gaaaacctta
     1741 gctttgatgc tcttcttgcg actctctcaa cgattttaac ttcgagatga ttaatctctg
     1801 tatattgatt tattttttca atattttttt gcagaaaaac cttattaaaa tccttaaaat
     1861 tctgataaag gtaaactttt tcgccattat ctattctgaa taattcaagt tcatccttta
     1921 atacatctac ttcaatgtcg aaaaatgtct ttttaccact gctgtattgt ttcctgataa
     1981 actgataaag catatttgaa ttttgatcag cgagtcttac agacgatatc aaaacctgag
     2041 tagtgtaaga atcctttaac atcgtgatat atggctcaat gacatgtgaa aaagcaatat
     2101 caatatatcc ttcactttca acataatcac aaaacgcagt caggttcata agcctgactg
     2161 catcagaagg taatttacct ctcttcttcg tatgtccaga gctttgattg ccctgaggtg
     2221 tgagaagttg atttcgagga atgcgaataa cactggcctg aagttcttct gccccctctt
     2281 tcaactgccg gtaagcacct gttacatcaa tatcagcgat aaatgcatat tcccgagccg
     2341 taatacgaaa ggttgccccc tcagataact cttcacgtga atcaatctgc gccatagcca
     2401 taaacaaaat tcttctggca gacaaaggca acgatgagaa tgtgctgtta atttcatttc
     2461 tgtggcgaat ttttgttttc tttgtaacgc tcagtaagtt catacattac ctgtggtttt
     2521 taagttctga tggtgaaatc ataatcacaa aggcggaaat atgtaaagtg gtttatccac
     2581 cttatgtatt tccaccaaaa cacggatcac atgaaagaat ttttatcatt tatattcaat
     2641 atgatagaaa tgcgcaggta tccacctttt aacgtcatac gttaaattag agcactataa
     2701 agaactaaag aaatgaagat acaaagatac gaatatctga ataaaaacag aaaaatgcgt
     2761 acccatccac ctttcagtgc gtacccatcc acctttcagt gcgcagccat ccacctttca
     2821 tgaaaattac agtgcgtacc catccacctt tcagtgcgta cccatccacc tttcattttg
     2881 aggcgacaaa aaacaacacc tatcttaatg aaattattga taaaaaagct ggcacaatgt
     2941 ttgcagaata agttaaagta agttaaagat cttaaagtag atctaccgat ctacatttaa
     3001 gatcttaaaa acaagaagtt taataagcaa aaaaacacaa aaaatctgtg gataactctg
     3061 ctcaaaacaa aaaattcaca gcgcctgtgg ataactttgc gtaaacccgg aggacagatc
     3121 actctgaacc aagacaacca cacataaaaa caatatgctc attttttaac cactgtgcgt
     3181 aagtgcctaa tgtaatccct gtgctattcc gcttcctcgc tcactaactc gctacactcg
     3241 gtcgttcggc tgcggcgagc ggtgtctcct ttctcaaaac cagaagagtt cagctcatac
     3301 gatccgttga ttgatccttt ttggatcttt cactgagaag ccctgaatcg ctttctgccg
     3361 cttcgttcga tacaaccatg gtgaaaccat gcgaaatcga aaaaatcgct cagaatgcga
     3421 tacagcgcgt tttagagggt atttacaaaa cagtcgttgc cttgaggaaa ttttgatcaa
     3481 gattgaactc ctatccggat catggcaaca gatcacagca acaaggccgc taaaaagcgt
     3541 tgagcggcgt taaaaaccgt taaaccgtag ctgactacaa cgaaacggga aaaaacgctc
     3601 agaacgcgtt acagagcgtt ttaaggcgat gtcggaaaga gccatatctc acctgttctg
     3661 atgtttttct caacataagc acttttaaga gtgcttttaa agatgctatt atgccaatga
     3721 gacaagcgga gggcttatga gctatcaaat tctgacaact acagcggcca gtattactga
     3781 cctgaaaaaa aatcctatgg gaaccgtagc tgaaggtgaa ggggacgctg ttgcgatcct
     3841 gaaccgaaac gaaccggcgt tctattgcgt tccaccaaaa ctttacgcct actatcggga
     3901 actcgctgaa gatgctgagt taaacgctgt tgctgatgag cgcatgaaaa acccggaaat
     3961 tgtgaaggtt aacctggatg acctatgaac tggcttttga ccgtagagca ctgaaggaat
     4021 ggcagaaact cggccacacc atccgtgaac aattcaaaaa gaaactggca gaacggctgg
     4081 aaaatccacg cgtacccgca gcccggttac atggtcatgc tgatcgctat aaaatcaaac
     4141 ttcgtgcatc tggctacaga cttgtatacc aagtcattga tgagaaagtc gttttactcg
     4201 ttattgccgt tggaagaagg gaaagcagcg aagtctatca gatcgcagat ttgcgctaag
     4261 attaatcatc aaatgattgg tgatgctgcc aacttactga tttagtgtat gatggtgttt
     4321 ttgaggtgct ccagtggctt ctgtttctat cagctgtccc tcctgttcag ctactgacgg
     4381 ggtggtgcgt aacggcaaaa gcaccgccgg acatcagcgc tatctctgct ctcactgccg
     4441 taaaacatgg caactgcagt tcacttacac cgcttctcaa cccggtacgc accagaaaat
     4501 cattgatatg gccatgaatg gcgttggatg ccgggcaact gcccgcatta tgggcgttgg
     4561 cctcaacacg attttacgtc acttaaaaaa ctcaggccgc agtcggtaac ctcgcgcata
     4621 cagccgggca gtgacgtcat cgtctgcgcg gaaatggacg aacagtgggg ctatgtcggg
     4681 gctaaatcgc gccagcgctg gctgttttac gcgtatgaca ggctccggaa gacggttgtt
     4741 gcgcacgtat tcggtgaacg cactatggcg acgctggggc gtcttatgag cctgctgtca
     4801 ccctttgacg tggtgatatg gatgacggat ggctggccgc tgtatgaatc ccgcctgaag
     4861 ggaaagctgc acgtaatcag caagcgatat acgcagcgaa ttgagcggca taacctgaat
     4921 ctgaggcagc acctggcacg gctgggacgg aagtcgctgt cgttctcaaa atcggtggag
     4981 ctgcatgaca aagtcatcgg gcattatctg aacataaaac actatcaata agttggagtc
     5041 attaccctga acaaactgtt aaggacaaaa aatgaagaaa ctcttaatac ctctgatagc
     5101 agctggtagt ctgctttatc ttcctgccag ccatgctgaa gatccctgca aagttattat
     5161 gtgcatggcg ggcaagctca ccggcgatag cggcggaagc gagtgtaaca gtgctgaagc
     5221 tgctttcttc aatatcgtta aaaagaacaa gcacggcttt ttacccaacc acacgaagga
     5281 tgctaggaag gcttttctta atgaatgccc ggataatggc gaaggtggaa gtaaccagtc
     5341 gatgataagc cagatcataa gtaaatacgg gaaagttcgc ttataggcga accaggaata
     5401 atctcaattt aaggagccaa cgtgaaaaaa ataatattaa gtgcattagc ctgtacagct
     5461 ctcttaactg gttgcgtcag ccaggataaa ggtaatgcga tgcagagcca gctgaataac
     5521 cagcaacgcc agattaacga attatccgtt cgtttgcagt ctgcggagtc ccggctatca
     5581 aagcaggaag aaaagctgcg caacgaactg ctgcaatcca gcggctattg ctatctgaat
     5641 ggcgcccgct actcgaccgg caccgtactt tacgggcgga tttgccagaa tcagtcaggc
     5701 agcgcttcgt ggcaggtata cagccgtcgc taaacatcag ggcggtttaa ccgccctttt
     5761 ctttaccctc agaaagccca ctataaccat gatccccttc cccactacag aaaaccttat
     5821 tctatgggct tgcagcgcca tcgtactgct tgccgttgta ttcttccggc gttcagtacg
     5881 gcacaggcga cacaaaagga agcagcaaag tgcgcggcgg gtgctggagc gcataaagac
     5941 gttgccgggc ttcccacaaa aaattaacta cctgagaaaa attgatcctt ttgtgtttga
     6001 agaactgttg ctggaaggat ttgaagcgca tggcttcaga accatcagaa acaaacgcta
     6061 taccggcgat ggaggcattg acggccaggt aataatagga aaatatcgct atcttattca
     6121 ggctaaacgc tatcgcggcc atattgcttt acagcacgta caggagttcg agaagttgct
     6181 taaacgtcat aactgtcgcg gtctgttttg ccataccggg aaaaccggcg caggttcaaa
     6241 atctgtcagt attgccagtg aacggatgga gattatcagc ggccagcgcc tgatagattt
     6301 gctcacgccc ggtagctcct tcactatcgc aaccgccccg cagacgatga tgaagcgtac
     6361 cgcagcaacg ctagaaacga gcaccattgt taaagatgcc ggggtaatga ctccaactta
     6421 ttgatagtgt tttatgttca gataatgccc gatgactttg tcatgcagct ccaccgattt
     6481 tgagaacgac agcgacttcc gtcccagccg tgccaggtgc tgcctcagat tcaggttatg
     6541 ccgctcaatt cgctgcgtat atcgcttgct gattacgtgc agctttccct tcaggcggga
     6601 ttcatacagc ggccagccat ccgtcatcca tatcaccacg tcaaagggtg acagcaggct
     6661 cataagacgc cccagcgtcg ccatagtgcg ttcaccgaat acgtgcgcaa caaccgtctt
     6721 ccggagcctg tcatacgcgt aaaacagcca gcgctggcgc gatttagccc cgacatagcc
     6781 ccactgttcg tccatttccg cgcagacgat gacgtcactg cccggctgta tgcgcgaggt
     6841 taccgactgc ggcctgagtt ttttaagtga cgtaaaatcg tgttgaggcc aacgcccata
     6901 atgcgggcag ttgcccggca tccaacgcca ttcatggcca tatcaatgat tttctggtgc
     6961 gtaccgggtt gagaagcggt gtaagtgaac tgcagttgcc atgttttacg gcagtgagag
     7021 cagagatagc gctgatgtcc ggcggtgctt ttgccgttac gcaccacccc gtcagtagct
     7081 gaacaggagg gacagctgat agaaacagaa gccactggag cacctcaaaa acaccatcat
     7141 acactaaatc agtaagttgg cagcatcacc caaataatta acactttgtc tcctttagtg
     7201 aaaatttttc cttcttcagg ctccagtagt aaataatgta tttggccaaa ttgatcggtg
     7261 agttttcctt cgcagggatt acctgaagtg gcttgatgac cggtaattaa cgccatgcta
     7321 ccaatatatt cttcttctgt aatagctgaa ctatggtcgc gtggaatcca gggcgcaact
     7381 attttaccgg tataatgcac cgcaataatc gtaaacagta aactgacagg aaccacgaac
     7441 aggttcgaca gtggtgattg ccagaccatg atgcaggcat gctgcaacag gatgccaata
     7501 agaccaaaaa aaccagccag taagcagaga acgacgagag ctggcaatct tccaatattg
     7561 agataaagaa gtgcctggct aatatgacct gtggttatgg aatcataatg gtcaagatgt
     7621 gcatcaagag caccggagag catgtgaccg cagataagcg caaaaatttc taacaggccg
     7681 atcaacagta caaaggaaat agcgaaaaga taaggggtgt tatagtcggc gaataaaatc
     7741 atatatcact ccaatgtcca tttagtgacg caatatttac tttgtaataa tttccttgct
     7801 tacatttatc tgtgttaata ataacacagg ttagagaagt tctggaagag aaagtctttt
     7861 aatatggttt tttatttttc actaaaaagt gtgatcgggg acaatatatt tacgcacgtt
     7921 atgtttaaag gcactacact gattggggaa tactgaaatc agaaaaagca tatggcctta
     7981 aaaggtatgc gataaccgga ggatgagctt atggataata gtcttaattc tttaaataat
     8041 ttcgatttcc tggcgcgtag ttttgccaga atgcacgcag aaggtcgccc ggtcgatatt
     8101 ctggccgtta ctggtaacat ggatgaagaa catagaacct ggttttgcgc acgttatgcc
     8161 tggtattgtc aacagatgat gcaggcaaga gagctggagt tagagcactg atataacggg
     8221 cctgatggcc cgttttagtg tttgtattag atactagtta taggtgattt tgaggatgac
     8281 gtctttacta aacggtccct caacaaccgt ttctgccggg ttcgcactca gttctgccct
     8341 gaatggtgcg gtgaccgaag gcgcactaga cgatgaaaaa gaagcaaatt gtgagaagcg
     8401 gttaaattcc gttgctgttc catcgttatg aaatatcttc aatagcagac cattacctat
     8461 atccagatgg ctataatcac tcaaaatatc cgtagtgaaa aaactcccta aaatattaaa
     8521 ctgctcagta caggccgcac ccggatcttt ggtcatgact acgctaaacg tctcactcac
     8581 gctggtattt ttaattgttc gggaatttat tttaccaaag tcgacaacgc catcagctgg
     8641 ttccagatca acattaacaa agcagtcaag gacatgaata ttatttaacc cacgaattct
     8701 gaaggtcaaa tttttgtctg tgtctatagt tcctgtgcca ctcatacctt taaactccag
     8761 ggcgatatat tcgtcaggta atacaagttt tttatttact ttattagcat ttttagccag
     8821 cttaatatac agctgtacac gtactggaaa agttactgac aattgaggtt caggaccacc
     8881 gctacagaca gcggggtttt tataggtatt actatatatc cctgcgcctt taacatcaat
     8941 gcactctggt gctgcgatag ctcgtgtgga ggttgaatca taatcgactc cattataggt
     9001 tacaccaaga gtatatgagt tttgattata aaagtcctga cctgatgcta acaacatcat
     9061 tatatttacc catgcagatg ggtattcttc tcctggtgat ggggcgggta gtgcattatt
     9121 acagaagaca ttcataaaat attcactgct ttgccagatt ttatcacctt ttttaccatc
     9181 gctggggata gcaattcttg gtacgtagcc tatgactacg gtattccccc cttcatgttc
     9241 agcatagcag ttcatcgccc atacaggtaa cgcactaaaa cccataataa atgcaattac
     9301 agttaataag tagcgcattt tatttatctc gtttggctac ggaaacatta caagaattat
     9361 tcactgttgg gcagtttatt tcataaaaac ttaagccacc aaaatcattg atataaccaa
     9421 cctgaaaatt tgatggaaca tttttaaact gcgtatctgc atggctaaaa gggaggacca
     9481 ttaagctttg cgtttgcggt aacatcgtga ccccatttgt tccgatataa cccagcgtaa
     9541 cgtgataagg cattttattg ttaaagcgga tcgaaccatc atttctacgg atgatttcaa
     9601 ctttttcgta agtcattacc ccatcgtctt ctaactcaat ggcttttggc cgccagaata
     9661 atttcaaagc gtgctgcata gtcacttgca tcacattttt tttatctgtt tttggcggta
     9721 tttctcgcac gttaaaatag aacagagttt ctctgtcctt aggaagttga gcaatttttt
     9781 cctgagttat cattctaatc tgaatttgct cattgggatt taaacgcacc ataggaggaa
     9841 ccgtaacaat aaagtcacga gatttattgc ctttcgtatc ttctatccat gtttgggcca
     9901 gatagggcag ttttttactt tgattagtaa gttttaatgc ttcgccttta tctgactcat
     9961 tcattattat acgggtacga tccgggatga tggcacccca ggcaatgctg ttcatgcagg
    10021 agagtaatac tcctgctgca atgatattct ttgtatttaa gtagcgcatt agtttcatcc
    10081 tgaattaatt ttagctaata cacggtaaaa taaccgttcc ggtggttaag ttgctgctct
    10141 tgggtggttg aataaagcaa gatttgtcac cccagcgtaa agtaagtttg ctgtcatcct
    10201 gaatgccgtt gaggtataca aagccgccat cagcaacaag tcctaattct ttgtggctgg
    10261 tttcatcttt tacgctaatc cccagtggtg ggtgactacc atctgccaga cgaataaccc
    10321 ccatgatttg atagcccttg cgggtatcaa gtttggcgta gccaatagca ccttcggtta
    10381 aggtggtggt gataaccgag ttttcgatat ccacattttc tggaagattt cgggtatcaa
    10441 cgcttaatga tgttgttatg taactgctga ctgatggaat aactccgatg ccataccgat
    10501 tagttaccgc gcgtttatta ttcaacggaa tatcttcagc gccgtctgcg tcgatcataa
    10561 aacgcgagtc agcactacca ctatagtcat ggaaagcggc gccgtggcga gttgcagtga
    10621 atgaaccgtt ccaactggca ttcagagaat agtagctgtc ccgctgtgat gtaccggaga
    10681 gatagagacg accgttctcg gtattatgct gataactggc gcgcagtgac gcctccatat
    10741 ctttaaattc gtcattgtcg cctgaagcag aaacgctcca ggaattattt cgatcggaag
    10801 agtcatacca cgaagcagta tgcgaaatgt tgttatcctg atttcgttgc ataccatagc
    10861 tcaatgttct actggttccc cagggaattg aaatatttag gtacagttga tcctggttgt
    10921 catcttccca gtttattcgg ctaaatgtta gtgaggtgga tacgtttttt aatgggccga
    10981 tatcaaaatc gcggctaagt gataatgaat aattcacatt actgctggcg tcccagtaag
    11041 tattacgagc cagacttacg tatgtattta atctcaatga ttcaagatac tggttaaacg
    11101 tgacaatata actctctttt tcattctgat aattcgtgta atggttagtg tcattgatat
    11161 attcattcat cgacacaaag tttttatcag agaaacggta gccagcgaaa gtgagctggc
    11221 taccggtaga ttcaaaacgt tttgaatagt tagcacggta gctataaccc gtttctttat
    11281 tctgattatg taactgagca tcagatcgcg tgacatcaaa ggataatgag cccaacgagt
    11341 taaggttaaa accaactccg gtagccaggg attgataatc acggtttgtt aatactgaac
    11401 caccatacag ggatacattg ttcagccaac cccatgagaa ttcaccggtc cagaagaagg
    11461 gctgttgtaa ggaatcgccg ccaacgctgg tcggtttccc cattgcagta gtgtagcgaa
    11521 tctgcccttt acgcgtcaga taaggaatgc tattagatgc aacttgccat tgcgtgacgc
    11581 gcccgtcctc ttcttcaacc ttgacgtcca gttgcccctg taatgtattg aataaattgg
    11641 taatagaaaa tgggcctgga ggaacgtttt cttgataaat aatacggttg ttctgtgaga
    11701 cagtgacctt tgcattcgtt tgtgcaacac ccgtgatttg cggtgcgtaa ccacgtagat
    11761 caggcggcaa catattctca tcgcttttca acgaaacgcc agaaaaatgg aaagagtcga
    11821 aaatagagga ttcagtatcg taggtgatgc tgccaactta ctgatttagt gtatgatggt
    11881 gtttttgagg tgctccagtg gcttctgttt ctatcagctg tccctcctgt tcagctactg
    11941 acggggtggt gggcactgtt gcaaagttag cgatgaggca gccttttgtc ttattcaaag
    12001 gccttacatt tcaaaaactc tgcttaccag gcgcatttcg cccaggggat caccataata
    12061 aaatgctgag gcctggcctt tgcgtagtgc acgcatcacc tcaatacctt tgatggtggc
    12121 gtaagccgtc ttcatggatt taaatcccag cgtggcgttg attatccgtt tcagtttgcc
    12181 atgatcgcat tcaatcacgt tgttccggta cttaatctgt cggtgttcaa cgtcagacgg
    12241 gcaccggcct tcgcgtttga gcagagcaag cgcgcgacca taggcgggcg ctttatccgt
    12301 gttgatgaat cgtgggatct gccacttctt cacgttgttg aggattttac ccagaaaccg
    12361 gtatgcagct ttgctgttac gacgggagga gagataaaaa tcgacagtgc ggccccggct
    12421 gtcgacggcc cggtacagat acgcccagcg gccattgacc ttcacggggt tgtaagccgg
    12481 aaccgccgaa atttccgtca actcctctaa ccccctgcca caagggcatc taggtcaatc
    12541 atcttgcggt cactctgaaa gatttaattc tttaccaacc ccaacccctg caacgaagtc
    12601 gggatcgtga gaaaccaata gcagagtgcc tgggtagtcc gccaggacct gagtgagcaa
    12661 ctgacgcgag gggtgatcca gatggttgtc cggttcatcg agcagcagca ggtcgggcag
    12721 ttgcgccagt accatcagca gacccagttt taacctctcc cccccactca gaccatgaca
    12781 aggcgtcaga gcacgatcgc cgcgcagggc tatgcccgcc agccgtgttc tcgcatcact
    12841 ctcggccagt gtcggagcca ctgtcaacag gttggcaagc gggctgagat cccgcgcaat
    12901 caggctgagc ccctgatcga ggcaagccat cttgccattg atgcgaaagc tgcccgtctc
    12961 gggctccagc accccacgca acatctgcag cagactcgat ttgccgcagc cattgcgacc
    13021 gccgagcaaa cagcgctcac cccgttgcag caccagatca acccgctggc catgccagcg
    13081 cacgtgagac gcagccgtaa tcaccagcgg ctgggcagcc cccttacgct cgatctcctc
    13141 cggccactgc atgatgagcg gctgcaccgc cgccagcgcg gctgctgcct cggtgcgggc
    13201 ctccacttgc gcactgtggc cccgctcgtg gcggtccagc actctgccaa gggatttgcc
    13261 tgcctgttcc gccttgaaat caagcaggat tttggcttgg gagccgcgct cacgccgccc
    13321 ctgactggct cgtctggccg cctgttcacg cactttttgc aagctgcgcg ccgccgcctt
    13381 ttcgcttcgc accgcttgcg ccaaactggt gctgcgggca gcctgtacct cggcctgctg
    13441 tgccagaaac tcgtcaaacg agaggcgggc actctgcaac ccctgctcgc tcagctccag
    13501 aatatgactg gtttgattga gcaggcgccg gtcgtggctc accagcaggt aacctccgcg
    13561 atgtctggcc agttgctggc ttagccagtc gatgccagag gcatcgaggt ggttgtctgg
    13621 ctcatcaagc aacagaaagt gagcaggcct cgcaaacagc gccagcaggc gcaaacgggt
    13681 gagctggcca ccgctcaggg tgctgaccgg gacatgggga tcgagtctga ggcctgcttc
    13741 tgccagcagg tcggcaagat cgctgcgcag gttccagcga tcagccagca actcgaaatc
    13801 gtcctgggcg cagctcccct gctcgatgcg ctcaagggcc cgtagcggct cgccccagcc
    13861 gataaggtcg gccacactgg ctgcgcagtc atgatcttga gccggtgcaa actgggacaa
    13921 ccagctgatg ggggtcaacc actcgatctt gccagaatcc gcgcggcgct ggcccgccag
    13981 cagttccagc agcaggctct tgccgatgcc gttacggccg atcagggtgc agcccgcagc
    14041 gggcaaagtg aaggtaaggg gcacagtaca ggcgacgaag ggcagctgca aagtgttgat
    14101 atgacatgcg atatgggtca tctgaactcc tgtaggccaa tgcggaagcc gcaggccaag
    14161 agctaaacca aatgacaggg acacgtgtcc actagtggga aaacagcaga ggaatcagca
    14221 tcaacctgtc acggtgagac gcacccgctc tccctggcgg gagaagaatc aaaagtgggg
    14281 tgcggacagg ttaaatgcgc atcggcgaaa tactctttac tgatgaaagt aaaatcatac
    14341 ttcagatagt aaaaaatgcc aaatgttccg gtatcactca cttgcatgtt gtatcaataa
    14401 gtcaagtgtc agtctgggct ataccctatc ctgatgtcag atcgttcggt taaaaagtgt
    14461 cagaataaag cagttaaatt aatttattga cacacataag gcaaggtcat agatttcttc
    14521 ctgacacttt taagctctgg aggtctcttg cagggacaac gcatcggtta catccgtgtc
    14581 agtagcttcg atcaaaaccc agaacggcaa ctggaacata tccaggtgga caaagttttc
    14641 atcgacaaag cctctgggaa agacactcag cgcccgcagc ttgacgtctt gctctccttt
    14701 gtacgtgaag gtgacacggt cgtggtacac agcatggatc gccttgctcg caacttggat
    14761 gatctgcgcc gacttgtaca gggactaacc cagcgccgcg tctgcattga attcgtcaag
    14821 gagcatctga ctttcaccgg cgaagattca ccgatggcaa accttatgtt gtctgtcatg
    14881 ggggcattcg ccgagttcga gcgagcgttg atccgggagc gtcaacgtga aggtattgcc
    14941 ttggcaaaac agcgtggcgc ttatcggggt cgaaagaaat cgctgtctga cccagagata
    15001 gcggaagtac ggcggcgagt cattgctggc gaaaagaaag cgacgcttgc ccgtgaattc
    15061 gggatcagtc gagaaacgct gtaccaatac ttgcgggtgc cggagtaact catggcgatt
    15121 gctaaaagcg agcgactaac agttctgtcg gacgccgagc aggaggcgct gtatggcttg
    15181 ccggactttg acgatgcgca gcgcctggaa tacctggcct tgaccgaaaa tgaactggcg
    15241 ctcgccagca gccggccaag catccatgct caggtctatt gcattctgca gatcgggtat
    15301 ttcaaagcca agaacgattt cttccgtttc tactggcgtg acgtcgagga tgattgcgct
    15361 ttcgtgctca gccgatattt tcaaggtgag gcattcgaga ccaagcagat ctccaaacac
    15421 gagcactaca gccaacgcgg taaaattgcc gcactattcg gttaccagcc gtgggcctcc
    15481 agctacctgc catcgcttga gcatcaagcc tcgctgatcg tgcggcgtga tgtgacgccc
    15541 aactttgttg ctgctgagct gatcgtctgg ctcaacgaac aaaagatcat tcgtccaggc
    15601 tataccaccc tgcaagcgct ggtcagcgaa gtcctgtccg ctgaacgtcg acgactggga
    15661 gccctgcttg cacaggtgtt ggatgaacca gccaagactt ccctgagtca gctcctggtg
    15721 cgcgacgata cgctgtcgca actggctgct ctcaaacaag acgccaagga tttcgggtgg
    15781 cgtcagatgg cccgagagcg tgacaagcgt accctactgg cgccattaca tgggattgcc
    15841 aaagggctgc tgcccaaact tgggatctct caacaaaacc tgctgtatta cgcgagtctg
    15901 gctaactttt ataccgtcca tgatttgcgc aacttgaagc cagagcagac ccagctctat
    15961 ctgttgtgct atgcttgggt acgctaccgg cagctcagcg ataacctggt cgatgcgatg
    16021 gcgttccaca tgcatcaact tgatcacgaa aaccgcgcgg gtgccaggga gtctttcgcc
    16081 gaagagcagg tcaaacgtca tcaggaaacg ccccaggttg gtcgcttgct gtccctgtac
    16141 gtggacgaca gtgtcgcgga tccgacgcca ttcggcgagg tgcgccagcg cgcctacaag
    16201 atcatggcca aagacgtgct gcaaaacacg gcgcagcgca tgagtgtcaa gccattgaat
    16261 aaactgacat tgcactggca ggcggtggat ggccaggcca aacgtatcag ccgtcatctc
    16321 cgaccgttgt ttgtcgcgct ggagttcgcc gctaccgatc cggacagcca gtggctggcg
    16381 gcactaacct gggttaaaag cgtgttcgcc aagcagctgc gtctgtcaca acgcccactc
    16441 gctgaatgcc cggcagccac cctacctaaa cgcttgcggc cctacctgct gagctttgat
    16501 gcagatggag agccgacagg gttacatgcc gaccgctacg agttctggtt gtaccgccaa
    16561 gtcacaaagc gtttccaatc tggcgagctt tatctcgatg acagtttgca acatcggcat
    16621 ttctccgatg agttggtgtc tatggaagag cacgctcagg agctggcaca gatggacatc
    16681 ccattcctgc gccaaccggt tgaaacacag cttgatgcct tggcctccga gctgcacaag
    16741 caatggcggg cattcaaccg tgagctgaaa caaggcaagc tgacacacct cgaatacgac
    16801 aaagacacgc aaaaactgac ttggcgcaag cccaaggggg aaaaccaaaa ggcgcgggag
    16861 caaacctttt acgatcaatt gccatactgt gatgtcgccg acgtgttccg cttcgtcaac
    16921 aaccaatgcc agttcctatc ggcactgact ccgctgcaac cacgttacgc caagaaggtt
    16981 gcggataccg atagcctgat tgcggtcatc atcgcccagg ccatgaacca cggcaacctg
    17041 gtcatggcgc gtaccagtga tattccatac cacgtcctgg agagcgccta tcagcagtac
    17101 ctgcggcaag catcgctaca cgaggccaac gacggcatca gcaatgccat tgccgcgctg
    17161 cctatcttcc cgtattactc gttcgacctc gatgtgctgt atggcgctgt cgatgggcag
    17221 aaattcagcg tcgagcggcc aaccgtgaaa gcgcgctact cgcgcaagta tttcgggcga
    17281 ggcaagggcg tggtggccta tacgctcttg tgcaaccaca tcccgttgaa tggatacctg
    17341 attggtgccc atgattatga ggcccaccat gtgttcgaca tctggtatcg caatacctct
    17401 gacattgtgc cagaggtcat tactggcgat atgcacagca tcaacaaggc caatttcgct
    17461 atcctgcact ggttcgggcg gcgtttcgag ccacggtata ccgacctcaa cgcccagttg
    17521 caggcgttgt attgtgtggg cgatccagca cggtatgaga agtgcctgat ccagccagtt
    17581 ggccaaatag atcggcagtt gattgtcagc gaaaaggcga acattgaccg gatcatcgcc
    17641 accctgggtt tgaaggagat gacgcaggga acgttgatcc gcaaactgtg cacctacacc
    17701 gcgccaaacc cgacgcggcg ggccatcttc gagttcgata agctcgtgcg cagcatctac
    17761 acgctacgct acctgcgcga cccccaattg gaacgtaacg tccatcgctc gcaaaaccgg
    17821 attgagtcat accatcagct acgttcaagc attgcgcagg tgggaggcaa gaaagaattg
    17881 acggggcgga ccgacattga gatcgagatc tgcaaccagt gcgccaggct gatcgccaac
    17941 gcagtcatct actacaactc ggccatcctg tcgcggttgc taacgaagtg cgaagcaaca
    18001 ggtaacgcca aggcgctcgc ggcgctcacc cagatatcgc cggcagcctg gcgacacatt
    18061 ttgctgaacg gacactatac ctttcagagt gacggcaagg tgattgatct ggatgccctt
    18121 gtggcagggg tggaactggc gtgacggaaa tttcggcggt tccggcttac aaccccttca
    18181 cgtaggtttc atccatgtgc cacgggcaaa gatcggaagg gttacgccag taccagcgca
    18241 gccgtttttc catttcaggc gcataacgct gaacccagcg gtaaatcgtg gagtgatcga
    18301 cattcactcc gcgttcagcc agcatctcct gcagctcacg gtaactgatg ccgtatttgc
    18361 agtaccagcg tacggcccac agaatgatgt cacgctgaaa atgccggcct ttgaatgggt
    18421 tcatgtgcag ctccatcagc aaaaggggat gataagttta tcaccaccga ctatttgcaa
    18481 cagtgccccc gcgagcgcca ggcgcacgag gccggcgcgc ggacccgcgc cacggcccac
    18541 gagcggacgc cgcagcagga gcgccagaag gccgccagag aggccgagcg cggccgtgag
    18601 gcttggacgc tagggcaggg catgaaaaag cccgtagcgg gctgctacgg gcgtctgacg
    18661 cggtggaaag ggggagggga tgttgtctac atggctctgc tgtagtgagt gggttgcgct
    18721 ccggcagcgg tcctgatcaa tcgtcaccct ttctcggtcc ttcaacgttc ctgacaacga
    18781 gcctcctttt cgccaatcca tcgacaatca ccgcgagtcc ctgctcgaac gctgcgtccg
    18841 gatcggcttc gtcgaaggcg tctatcgcgg cccgcaacag cggcgagagc ggagcctgtt
    18901 caacggtgcc gccgcgctcg ccggcatcgc tgtcgccggc ctgctcctca agcacggccc
    18961 caacagtgaa gtagctgatt gtcatcagcg cattgacggc gtccccggcc gaaaaacccg
    19021 cctcgcagag gaagcgaagc tgcgcgtcgg ccgtttccat ctgcggtgcg cccggtcgcg
    19081 tgccggcatg gatgcgcgcg ccatcgcggt aggcgagcag cgcctgcctg aagctgcggg
    19141 cattcccgat cagaaatgag cgccagtcgt cgtcggctct cggcaccgaa tgcgtatgat
    19201 tctccgccag catggcttcg gccagtgcgt cgagcagcgc ccgcttgttc ctgaagtgcc
    19261 agtaaagcgc cggctgctga acccccaacc gttccgccag tttgcgtgtc gtcagaccgt
    19321 ctacgccgac ctcgttcaac aggtccaggg cggcacggat cactgtattc ggctgcaact
    19381 ttgtcatgct tgacacttta tcactgataa acataatatg tccaccaact tatcagtgat
    19441 aaagaatccg cgcgttcaat cggaccagcg gaggctggtc cggaggccag acgtgaaacc
    19501 caacagaccc ctgatcgtaa ttctgagcac tgtcgcgctc gacgctgtcg gcatcggcct
    19561 gattatgccg gtgctgccgg gcctcctgcg cgatctggtt cactcgaacg acgtcaccgc
    19621 ccactatggc attctgctgg cgctgtatgc gttgatgcaa tttgcctgcg cacctgtgct
    19681 gggcgcgctg tcggatcgtt tcgggcggcg gccggtcttg ctcgtctcgc tggccggcgc
    19741 tgctgtcgac tacgccatca tggcgacgac gcctttcctt tgggttctct atatcgggcg
    19801 gatcgtggcc ggcatcaccg gggcgactgg ggcggtagcc ggcgcttata ttgccgatat
    19861 cactgatggc gatgagcgcg cgcggcactt cggcttcatg agcgcctgtt tcgggttcgg
    19921 gatggtcgcg ggacctgtgc tcggtgggct gatgggcggt ttctcccccc acgctccgtt
    19981 cttcgccgcg gcagccttga acggcctcaa tttcctgacg ggctgtttcc ttttgccgga
    20041 gtcgcacaaa ggcgaacgcc ggccgttacg ccgggaggct ctcaacccgc tcgcttcgtt
    20101 ccggtgggcc cggggcatga ccgtcgtcgc cgccctgatg gcggtcttct tcatcatgca
    20161 acttgtcgga caggtgccgg ccgcgctttg ggtcattttc ggcgaggatc gctttcactg
    20221 ggacgcgacc acgatcggca tttcgcttgc cgcatttggc attctgcatt cactcgccca
    20281 ggcaatgatc accggccctg tagccgcccg gctcggcgaa aggcgggcac tcatgctcgg
    20341 aatgattgcc gacggcacag gctacatcct gcttgccttc gcgacacggg gatggatggc
    20401 gttcccgatc atggtcctgc ttgcttcggg tggcatcgga atgccggcgc tgcaagcaat
    20461 gttgtccagg caggtggatg aggaacgtca ggggcagctg caaggctcac tggcggcgct
    20521 caccagcctg acctcgatcg tcggacccct cctcttcacg gcgatctatg cggcttctat
    20581 aacaacgtgg aacgggtggg catggattgc aggcgctgcc ctctacttgc tctgcctgcc
    20641 ggcgctgcgt cgcgggcttt ggagcggcgc agggcaacga gccgatcgct gatcgtggaa
    20701 acgataggcc tatgccatgc gggtcaaggc gacttccggc aagctatacg cgccctagga
    20761 gtgcggttgg aacgttggcc cagccagata ctcccgatca cgagcaggac gccgatgatt
    20821 tgaagcgcac tcagcgtctg atccaagaac aaccatccta gcaacacggc ggtccccggg
    20881 ctgagaaagc ccagtaagga aacaactgta ggttcgagtc gcgagatccc ccggaaccaa
    20941 aggaagtagg ttaaacccgc tccgatcagg ccgagccacg ccaggccgag aacattggtt
    21001 cctgtaggca tcgggattgg cggatcaaac actaaagcta ctggaacgag cagaagtcct
    21061 ccggccgcca gttgccaggc ggtaaaggtg agcagaggca cgggaggttg ccacttgcgg
    21121 gtcagcacgg ttccgaacgc catggaaacc gcccccgcca ggcccgctgc gacgccgaca
    21181 ggatctagcg ctgcgtttgg tgtcaacacc aacagcgcca cgcccgcagt tccgcaaata
    21241 gcccccagga ccgccatcaa tcgtatcggg ctacctagca gagcggcaga gatgaacacg
    21301 accatcagcg gctgcacagc gcctaccgtc gccgcgaccc cgcccggcag gcggtagacc
    21361 gaaataaaca acaagctcca gaatagcgaa atattaagtg cgccgaggat gaagatgcgc
    21421 atccaccaga ttcccgttgg aatctgtcgg acgatcatca cgagcaataa acccgccggc
    21481 aacgcccgca gcatcgcgac cgtcatcggt gagaagttcg gcaggtattg ggtggtgaca
    21541 atgtaggtgc tgccccaaat ggcaggtgct atcgctgtga acaataaatc gggcgtgcgt
    21601 aatgacatgc gattacaaga cctccgctac ggcgatgggt ttttgagagt tgcaggcgcc
    21661 gcgatgaccg caccctgact tggactggcc ctgcctcgca ccaatccacc tgcggctatg
    21721 gccgcgatca tgcgaggccc gaaaattaca cgacctgtac acgtccgccg aacccggaac
    21781 gcaacagagc ttcgtgggca tcccgatccg ggtcgtagca gcagtcctgg accaaacgca
    21841 cgtcgtagtc cgcatcacta gcccaggcga cgcttgaaag aacaacgccg gtggtgctta
    21901 tcccggccat gacaagcgtg cttacgccgc gcgtccgaag gtcggcgtcg agcgccgtgc
    21961 cataaaagac gctggctcgc ggacatgcat agaaaaggtc gccccgttcg atcgcaagcc
    22021 cttcgacggg cagaccggtg cgaaatcgtc cgctcgggag atacggtgag atttggcgat
    22081 tcgtcgccgg cggcgcatgt tcatactctt cgccgagcga aaagttgggg aacagcacgg
    22141 gccggccgcg caaggcgtgc tggcagccgt gcagaccctg cgtgagatga acgccgacaa
    22201 cctgcgcaag gtgccggccg atgcacccac ggccttcatc aagccgcgct ggaagccgct
    22261 ggtgatcacc ccggaaggcc tcgaccggaa attctacgaa atctgcgccc tgtccgagct
    22321 gaagaacgcc ctgcgctccg gcgacatctg ggtcaagggc tcgcggcagt tccgcgactt
    22381 cgacgactac ctgctgccgg ccgagaagtt cgccgcactc aagcgcgagc aggccctgcc
    22441 cctggcgatc aacccgaaca gcgaccagta cctggaagag cgtttgcagc tgctggacga
    22501 gcagttggcc accgtcaccc gcctggccaa ggacaacgag ctgcccgatg ccatcctcac
    22561 cgagtcaggg ctgaaaatca ccccgctgga tgcggcggtg ccggatcggg cgcaggcgct
    22621 gatcgaccaa accagccagt tactgccgcg catcaagatc accgaactgc tgatggacgt
    22681 ggacgactgg acgggcttca gccgccactt cacccacttg aaggacgggg ccgaggccaa
    22741 agacaggacg ttgctgctgt ccgcaatcct cggtgatgcg atcaacctcg ggctgaccaa
    22801 gatggccgag tcgagccccg gcctgaccta cgccaagctg tcctggctgc aagcctggca
    22861 catccgcgac gaaacctatt cggcggcctt ggccgagctg gtcaaccacc agtatcgcca
    22921 cgcctttgcc gcccactggg gcgacggcac gacctcatcc tccgatggcc agcgcttccg
    22981 cgcgggtggc cggggcgaga gcaccgggca cgtcaacccg aagtacggta gcgagccggg
    23041 acggctgttc tatacccata tctccgacca gtacgcgccg ttcagcaccc gcgtggtgaa
    23101 tgtcggcgtc cgcgattcca cctatgtgct cgacggcctg ctgtaccacg agtccgacct
    23161 gcggatcgag gagcactaca ccgacacggc cggcttcacc gatcacgtct ttgccctgat
    23221 gcacctgcta ggcttccgct tcgcgccgcg catccgcgac ctcggcgaaa ccaagctgta
    23281 cgtgccgcag ggcgtgcaag cctacccgac gttgcgcccg ctgatcggcg gcaccctgaa
    23341 catcaagcac gtgcgtgccc actgggacga catcctgcgc ctggccagct cgatcaagca
    23401 gggcaccgtc accgcctcgc tgatgctgcg caagctcggc agctacccgc gccagaacgg
    23461 actggccgtg gccctgcgcg agctgggccg gatcgagcgc acgctgttca tcctggactg
    23521 gctgcaaagt gttgaactgc gccgccgcgt gcatgccggc ctgaacaaag gtgaggcgcg
    23581 caactcgctg gccagggcgg tgttcttcaa ccgccttggg gaaatcaggg atcggagctt
    23641 cgagcagcag cgctaccggg ccagcggcct caacctggtg acggcggcta tcgtgctgtg
    23701 gaacacggtg tacctggaac gcgccaccca ggggttggtc gaggccggca agccggtgga
    23761 cggcgagctg ctgcaattcc tgtcgccgct gggctgggag cacatcaacc taaccggcga
    23821 ttacgtctgg cggcagagcc gcagactgga agacgggaag tttcggccct tacggatgcc
    23881 cggaaaacct tagcgtacga ttttttccga attctgcggg ctccccactt acaccgcttc
    23941 tcaacccggt acgcaccaga aaatcattga tatggccatg aatggcgttg gatgccgggc
    24001 aaccgcccgc attatgggca ttggcctcaa cacgatttta cgtcacttaa aaaactcagg
    24061 ccgcagtcgg taacctcgcg catacagccg ggcagtgacg tcatcgtctg cgcggaaatg
    24121 gacgaacagt ggggctatgt cggggctaaa tcgcgccagc gctggctgtt ttacgcgtat
    24181 gacaggctcc ggaagacggt tgttgcgcac gtattcggtg aacgcactat ggcgacgctg
    24241 gggcgtctta tgagcctgct gtcacccttt gacgtggtga tatggatgac ggatggctgg
    24301 ccgctgtatg aatcccgcct gaagggaaag ctgcacgtaa tcagcaagcg atatacgcag
    24361 cgaattgagc ggcataacct gaatctgagg cagcacctgg cacggctggg acggaagtcg
    24421 ctgtcgttct caaaatcggt ggagctgcat gacaaagtca tcgggcatta tctgaacata
    24481 aaacactatc aataagttgg agtcattacc gcaaacttaa gagcattcct cacatcaggc
    24541 atgcctacgt tttcaggaaa agttcgaagc attcttcttt cagatgtttc taaacactgg
    24601 aaattgagag atttagctga ttatctttac atgagcgaga gcttaataaa gaaaaagcta
    24661 ttattagaaa acacttcttt cagtaaaata ttacttgata ccaggatgtc gcttgccatt
    24721 aaattgttaa agcaaaatca tccgataaaa tatgtgtctg aaatgtgtgg gtttagtagt
    24781 acatcgtact ttgtatgcct tttcagacaa tactatgagt gtacaccacg taaatatcta
    24841 aagcattaga gaacacctgc ctttacttcc ttattttacc aataatttca gctaagagat
    24901 tttgatctaa atgttcgtta atcgttaagc ttacttctgt acatgccctt atatagatgt
    24961 tctgaacggt gttatttctg acaatggact taaatgtcag taacgccatt acacagttgg
    25021 ccaacgcgaa cagcagtatt ggtcgcaaca aaaaatgaaa gaaaaatccc ccaaatgaag
    25081 ggaaaaaacg gaagaacagt cgaacgacag gcacaaaaaa acccgactgg taatcgggct
    25141 ttttcgtgtt ttccagtcct gagttggtgg ctctggctgg agtggaactg tcttcaacca
    25201 acaccgaaga acaaccgata tgacaggcag tgtaagagta agtatcggga gtctgctcca
    25261 tataatggag taaacggatg attgataaag caatcatcgt tctgggggca ctaatctcac
    25321 ttcttgaatt gattcgctac cttctccagt tgatgggctg atggcggaaa cgtaactaag
    25381 gccgggggct aaccacctcc ggcctttaat aaaaacatat tttagttaca atattcaaat
    25441 gcattgacta aacaccacac acccaatctc ctgagttaat atcctgcctt gcaggtatat
    25501 tagcccccta ctcctctact tactggattt ctgcccggtt ttacgtttta gcgcattact
    25561 gattgtgcca cggctgcacc ctagcacttt ctgtacctgt gtccaggagc taccgctttc
    25621 tatcaacctg ataattgcat catgtctggc ttgattaggt ttacgccctt tatatttgcc
    25681 gtctttcttt gctttctcta tcccttgttt ctggcgttcc cgacgttgtt catagtccct
    25741 tctggcaact gcggccagca tatccagcag catatcgtta atggctgcaa acaaccggct
    25801 gtcaaactca ctcataccag aattaatcca tgtcgtcggc acattcaccg ccataacccg
    25861 gatgtctttc tgccggatca ttttcttcaa cgtattccag tcttcccctg acaggcggga
    25921 aagtctgtcc acatcctcga taagcaaaat gtcattttgt tggcaatctc tcagaagacg
    25981 aaaaagttcc gggcgctcaa gacgggtgcc ggattcattc tcaatatagt agctgcaaat
    26041 gatcaggcct ctttccctgg caaaagcctc gatagtttcc agggcacgcg aagcatcctg
    26101 ttctgctgtt gaagctctca gataagcgcg gacaaagctt gttggtacat ttgaagtcat
    26161 attacagaac cggttcacat aaggttacac cactataccc aaaatgcacc agaaagtaga
    26221 tcgacaggaa aggcacactc aaaaagaacc agaaaacaga cctccgacca taaagtgaca
    26281 atgtcacaca gaaatttaca acataaagat gatgtaaaga cggtctcaga tttaccacgt
    26341 aaagccacta taaatacaca caaatcttta cacaataaag ataacataaa tacaagctta
    26401 tctttatttg tgctttacat ggtaaatcat gtgtaatata aagacactgt aaagacaaaa
    26461 taatctttac gaacaacaca caatgtaaag ataagacagt ctttacataa tctttacgtc
    26521 ataaagagag gaattctgaa atgggtaaaa tcttgcttgt cgtatcagat aagggtggtg
    26581 taggcaaaag cacctacgtg gctaacacag gctcaatgct ggtcaataaa ggtaagtcgg
    26641 taattatcct caagacagat aaaaaccacg atctgttgag ctggaacgaa aagcggacag
    26701 ataacggctt actcactatc ccggttcacg cagcttacgg aaacgtcagc aatgaaatta
    26761 agcgacttag taagctgtgt gaagtcctca ttgtcgattg ccccggccac gacagccagg
    26821 agttccgtag cgccctgaca gtatcagata tcgtggtaac actggttaag ccgtcctctg
    26881 atttcgaaag cgaaactctg accagtgtta cagagaaaat ccgcaccgct cagaaagcta
    26941 acgcagccct ggaaccgtgg gtgctgttca ccagaatcaa ctcagcgaaa ccccgccaca
    27001 gaaaagcggc cattgatctg gataaattac tgcgttcgga caacatctgg attcaacctc
    27061 ttaaaacccg gatatccgag ctggatgtgt atgaaagcgc ctgtaatgaa ggggcagggg
    27121 tacatgacgt tagccgggca tcaagccttt caacagcgaa agcacagatt gaactagtgg
    27181 cgcaggaaat tggaatttta tagtaatatc aataccatct ataaatcttt acattgtaaa
    27241 gataatataa agatagtatg atatttacta ttaatgcata ccgtacaagg ggaaattgtc
    27301 atggctttaa aactaaaccg tccgaacatc gatgaatctc agcaaccagc aacaaacgca
    27361 gaaaccgcgc gttttatctc cggcgctacc agagcgcctg tagtgggtaa accaaagttg
    27421 atgaacttca gactttcagc ccattttgag gacatactgg aagctgaagc tatcagaaca
    27481 gggcagagca aaacaaccgt tctcaaagcg gcgctggcga tgtacaacag ccaggatgaa
    27541 aacataaaga atcactggtt gcttgagtct gcaaaattct aaccatagaa gcatggatga
    27601 aaatctatga gatatttaac cgaattgatc tgtgacctct acaggttgac agatcaccat
    27661 aaactgacca cagcaacgtt taaaaatctt gctgatatta agttatttaa acctacagca
    27721 gatgcagaca ccatattgca gcttgaaaat atatttagcg aatactcact gagatatatt
    27781 gacaaggatc tggctgaaat tctgagcaac ttaataacta cagataagaa acactctctg
    27841 gacttcgatt ttaacaaaat acaatctctg actgactcga aaagtttcgg ttg
//
