LOCUS       NZ_CP063339             2033 bp    DNA     circular CON 17-JUN-2023
DEFINITION  Escherichia coli strain BS74R-D plasmid pBS74R-D_7, complete
            sequence.
ACCESSION   NZ_CP063339
VERSION     NZ_CP063339.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN16387362
            Assembly: GCF_016942475.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2033)
  AUTHORS   Moser,A.I., Campos-Madueno,E.I. and Endimiani,A.
  TITLE     Complete genome and plasmid sequences of MDR Escherichia coli
            acquired by Swiss travelers in Laos
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2033)
  AUTHORS   Moser,A.I., Campos-Madueno,E.I. and Endimiani,A.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-OCT-2020) Institute for Infectious Diseases,
            University of Bern, Friedbuehlstrasse 51, Bern 3001, Switzerland
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Flye v. 2.7.1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 445x
            Sequencing Technology :: Illumina NovaSeq; Oxford Nanopore MiniION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 06/17/2023 06:02:34
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,200
            CDSs (total)                      :: 5,077
            Genes (coding)                    :: 4,781
            CDSs (with protein)               :: 4,781
            Genes (RNA)                       :: 123
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 90
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 82 of 296
            Pseudo Genes (incomplete)         :: 207 of 296
            Pseudo Genes (internal stop)      :: 46 of 296
            Pseudo Genes (multiple problems)  :: 38 of 296
            Pseudo Genes (short protein)      :: 4 of 296
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP063339.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2033
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="BS74R-D"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pBS74R-D_7"
                     /geo_loc_name="Switzerland"
                     /collection_date="2018"
                     /collected_by="Institute for Infectious Diseases Bern"
     gene            754..1722
                     /gene="mobV"
                     /locus_tag="IN878_RS25570"
                     /old_locus_tag="IN878_25585"
     CDS             754..1722
                     /gene="mobV"
                     /locus_tag="IN878_RS25570"
                     /old_locus_tag="IN878_25585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000024818.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobV family relaxase"
                     /protein_id="WP_000024818.1"
                     /translation="MANYAIMRCKKLTGMGSVASALQHCYRERETPNANAERTPENYCS
                     VSKSTDQAMGRVRELLPEKRRKDAVLAVEYVMTASPEWWKEATPRQQAEFFARSEQWLE
                     KKYGKDRVVAAVVHRDEATPHLSAFVVPLTQDGRLSAKEFIGGRSKMREDQSTYAESVK
                     KLGLERGIEGSRATHQTVQHYYESINRGTRSQVSISPEALEPRVLRKGIFTKDVEDQAA
                     IAKRLSHAVNDGFAGTIAMASQSAQNAKRARELQKTMDAQQKRLQSVTEPFKGLSREQM
                     TEILMMAQRFKQQNQEKEKQQRIEREKQRQTRSRGMGGMER"
                     /name="mobV"
     gene            complement(1786..1974)
                     /locus_tag="IN878_RS26070"
     CDS             complement(1786..1974)
                     /locus_tag="IN878_RS26070"
                     /inference="COORDINATES: protein motif:HMM:NF024854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="WP_251301863.1"
                     /translation="MQKTHVSRAVRALLDVGVIFEGPKVGRSKTYRLNEQFGWKGTVSN
                     HKKALKNGLSVIQGGRT"
                     /name="replication protein"
     Typing          746..1719
                     /name="MOBV"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="95.077"
                     /coverage="96.0"
                     /evalue="0.0"
ORIGIN
        1 cagatcagcg agcatgagca ttagaacttt aaattgctcg cccgttagct ccttagcgag
       61 gatctttaat gcgtcttggt tcatagtgaa atgcctctga aatgacgatt tttgcttggg
      121 tctgattacc gcgacgaagc cgccaacaat ctcaccttga tcgttaatct gggtgattct
      181 ctgggtgtcc ttgctcatcg aaaataacct atcttgagtt accaataatg gtaacagtgt
      241 taccattatt ggtaactatg tcaaatgtaa cccctcgcca tacatacgtt tttttatcgc
      301 tccttctgta tcttataacg cgtgcgcgcg gtttagctct gaataccaac gttttttagc
      361 gacggagtgt acggatttct atgtacgggg agtggaaatt ccgcttcctc gctcattcgc
      421 tcactacgtt cggtcatgag tctgcggcga gcggttcctg ctctttcaaa agcgatattc
      481 attgcctgat ttcgtcaagg gcgagcgaag cgagtgccat ttacccttga cgacactccg
      541 gataaaacag gcttggcagt ggtgggggtg gcgcggagcg acgccttcca cctgccagac
      601 cgccgttgat tttgaccttc tggcagcttg ctgcccgaac gattttgcga agcaaagtct
      661 agtgagtaca ctatcatctt gatagtggcc cgccggaggt atcgcaatgc gctcccccgc
      721 ctggctgaat gaatgccgga atcagggggg aacatggcga actacgcgat aatgcgctgc
      781 aaaaagctga cgggaatggg aagcgtggcc agcgcgttgc agcactgcta ccgagagcgt
      841 gagacgccga acgctaacgc tgaacggaca ccagaaaact actgctctgt gtccaaatca
      901 acggatcagg caatggggcg tgtgcgggag ctgctgccgg aaaaacgccg taaagatgcg
      961 gtactggccg ttgaatacgt tatgacagca agccctgagt ggtggaaaga ggccacacca
     1021 cggcagcagg cggagttttt cgcccgctct gagcaatggc tggagaagaa atacgggaag
     1081 gatcgtgttg tggcggcggt ggttcaccgg gacgaggcca ccccgcacct gtctgcgttc
     1141 gtggtgccat tgacgcagga tggacggctg tccgcgaaag agtttattgg cgggcggtca
     1201 aagatgcgtg aagatcagag cacctacgct gaatcagtaa aaaaactggg tcttgagcgc
     1261 ggcatcgagg gaagccgcgc cacgcatcag acagttcagc actactacga atcgataaac
     1321 cgtggcaccc gtagccaggt atcgatctcg ccggaggcgc tggagccgcg tgtattgcgg
     1381 aagggcattt tcaccaaaga cgtggaagat caggcagcca tagccaaacg tctaagccac
     1441 gcagtgaacg atggctttgc aggaaccatc gctatggcct ctcagagcgc ccagaacgca
     1501 aaaagagcgc gtgagctaca gaagactatg gatgcgcagc agaaacgcct acagagcgtt
     1561 acagagcctt ttaaggggct ttccagagag cagatgaccg aaatcctgat gatggctcag
     1621 cggtttaagc agcaaaacca ggaaaaggaa aaacagcagc gtattgaacg tgaaaaacag
     1681 cgacagacgc gatcgcgtgg catgggtggt atggagcgtt agttttgatg cgatgcagga
     1741 tagggctaac gccgcaaagt gacgtcattt gcggcattcc agagatcagg ttctgccgcc
     1801 ctgtataacg ctgaggccgt ttttaagggc ttttttgtga ttgcttactg ttcctttcca
     1861 gccgaattgc tcgttcaacc gataggtttt ggatctacct actttcggac cttcaaagat
     1921 cacgcctaca tcaagaagag cacgaacagc gcggcttacg tgggtctttt gcatctccaa
     1981 tgcatccgct atatcagcct gtgcaatttg gatgaaattc tcgtaatcca gat
//
