LOCUS       NZ_CP063338            10567 bp    DNA     circular CON 17-JUN-2023
DEFINITION  Escherichia coli strain BS74R-D plasmid pBS74R-D_6, complete
            sequence.
ACCESSION   NZ_CP063338
VERSION     NZ_CP063338.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN16387362
            Assembly: GCF_016942475.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10567)
  AUTHORS   Moser,A.I., Campos-Madueno,E.I. and Endimiani,A.
  TITLE     Complete genome and plasmid sequences of MDR Escherichia coli
            acquired by Swiss travelers in Laos
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10567)
  AUTHORS   Moser,A.I., Campos-Madueno,E.I. and Endimiani,A.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-OCT-2020) Institute for Infectious Diseases,
            University of Bern, Friedbuehlstrasse 51, Bern 3001, Switzerland
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Flye v. 2.7.1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 445x
            Sequencing Technology :: Illumina NovaSeq; Oxford Nanopore MiniION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 06/17/2023 06:02:34
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,200
            CDSs (total)                      :: 5,077
            Genes (coding)                    :: 4,781
            CDSs (with protein)               :: 4,781
            Genes (RNA)                       :: 123
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 90
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 82 of 296
            Pseudo Genes (incomplete)         :: 207 of 296
            Pseudo Genes (internal stop)      :: 46 of 296
            Pseudo Genes (multiple problems)  :: 38 of 296
            Pseudo Genes (short protein)      :: 4 of 296
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP063338.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..10567
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="BS74R-D"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pBS74R-D_6"
                     /geo_loc_name="Switzerland"
                     /collection_date="2018"
                     /collected_by="Institute for Infectious Diseases Bern"
     gene            complement(join(10504..10567,1..617))
                     /locus_tag="IN878_RS25495"
                     /old_locus_tag="IN878_25510"
     CDS             complement(join(10504..10567,1..617))
                     /locus_tag="IN878_RS25495"
                     /old_locus_tag="IN878_25510"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PDC sensor domain-containing protein"
                     /protein_id="WP_251301862.1"
                     /translation="MRIKKPHLRPIVITLSVGGILLTSIFLIAVIMVFQRENIEKDLLY
                     ANSSYAMKMSDVMSGYIEMAQGELAYGARKIKSTNDIDSLKDEADRLRLQSGMFNSVFV
                     VSNDAVVLATSPESLDLVGTRLNSSASKLAIEEKKPFISSPFKSVTGNLIILLSHPIYD
                     KNGNYIGHVGGSIYLKKHSLFSDVLSRHFFKSDTEISIVSDDGVNPEAGRFGEDSSWMI
                     LAGK"
                     /name="PDC sensor domain-containing protein"
     gene            complement(963..1178)
                     /locus_tag="IN878_RS25500"
                     /old_locus_tag="IN878_25515"
     CDS             complement(963..1178)
                     /locus_tag="IN878_RS25500"
                     /old_locus_tag="IN878_25515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_015589619.1"
                     /translation="MTELEKQLLSALEQLQQDYSKRLDEWESAFAEWRTMSGLMQRENA
                     ALSERVTGLSRQVQSLSEQLRRLSSD"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(1182..1667)
                     /locus_tag="IN878_RS25505"
                     /old_locus_tag="IN878_25520"
     CDS             complement(1182..1667)
                     /locus_tag="IN878_RS25505"
                     /old_locus_tag="IN878_25520"
                     /inference="COORDINATES: protein motif:HMM:NF016710.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_012565228.1"
                     /translation="MNSLLTLAKDLEQKSKAQQQNTGEMLKAAFSEHEKSVKAELNESA
                     KRISAAILDHDRKLSSAMSQRTKGMVRMVSQTWLTIVLVSALLIASSAGILWWQGQQIL
                     DNYTTIREQKSTQAMLSERNSGVQLSTCGEQRRRCVKVNPEAGRFGEDSSWMILAGK"
                     /name="MbeB family mobilization protein"
     gene            complement(<1702..2136)
                     /locus_tag="IN878_RS26060"
                     /pseudo=""
     CDS             complement(<1702..2136)
                     /locus_tag="IN878_RS26060"
                     /inference="COORDINATES: protein motif:HMM:NF015397.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(2345..2668)
                     /locus_tag="IN878_RS25515"
                     /old_locus_tag="IN878_25530"
     CDS             complement(2345..2668)
                     /locus_tag="IN878_RS25515"
                     /old_locus_tag="IN878_25530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_007772081.1"
                     /translation="MLTMWVTEDEHRRLLERCDGRQLAAWMRQTCLDEKPARSGKLPSI
                     SPALLRQLAGMGNNLNQIARRVNAGGGTGHDRVQIVAALMAIDAGLERLRHAVLEKGTD
                     DDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            complement(3726..5282)
                     /locus_tag="IN878_RS25520"
                     /old_locus_tag="IN878_25535"
     CDS             complement(3726..5282)
                     /locus_tag="IN878_RS25520"
                     /old_locus_tag="IN878_25535"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015589620.1"
                     /GO_function="GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]; GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="WP_226426401.1"
                     /translation="MRIKKPHLRPIVITLSVGGILLTSIFLIAVIMVFQRENIEKDLLY
                     ANSSYAMKMSDVMSGYIEMAQGELAYGARKIKSTNDIDSLKDEADRLRLQSGMFNSVFV
                     VSNDAVVLATSPESLDLVGTRLNSSASKLAIEEKKPFISSPFKSVTGNLIILLSHPIYD
                     KNGNYIGHVGGSIYLKKHSLFSDVLSRHFFKSDTEISIVSDDGTVIFNNDNSAVGRPMV
                     MSDDLKSKLTESDRGEGAFFSSGNRYLLGYAHMKNPAWNVLVYSHADSATTILITYAKS
                     VLVLLIVIIITFSLLSYFIASRISLPLEKLAISTNAKDIGASLVYIKAINAWYAEADRL
                     KKALFTHVKVMMERVDSLNEMALKDPLTGVNNRLGFLSKIQNHKRDSGYSVIVIDVDFF
                     KEINDSFGHGVGDEVLVSLAHIINSCCRSEDVVCRFGGEEFVVFLPNTSVVTAKCVAER
                     IRSVIESTVFPNNLRVTISAGVATQDDPLGRIDFLLKNADDALYQAKREGRNKVIVYSA
                     I"
                     /name="sensor domain-containing diguanylate cyclase"
     gene            complement(5628..5843)
                     /locus_tag="IN878_RS25525"
                     /old_locus_tag="IN878_25540"
     CDS             complement(5628..5843)
                     /locus_tag="IN878_RS25525"
                     /old_locus_tag="IN878_25540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_015589619.1"
                     /translation="MTELEKQLLSALEQLQQDYSKRLDEWESAFAEWRTMSGLMQRENA
                     ALSERVTGLSRQVQSLSEQLRRLSSD"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(5847..6332)
                     /locus_tag="IN878_RS25530"
                     /old_locus_tag="IN878_25545"
     CDS             complement(5847..6332)
                     /locus_tag="IN878_RS25530"
                     /old_locus_tag="IN878_25545"
                     /inference="COORDINATES: protein motif:HMM:NF016710.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_001086854.1"
                     /translation="MNSLLTLAKDLEQKSKAQQQSTGEMLKAAFSEHEKSVRAELSESE
                     KRISAAILDHDRKLSAAMSQRTKGMLRMVSQTWLTIVLVSTLLTASGASILWWQGQQIL
                     DNYTTIREQKRTQAMLSERNSGVQLSTCGEQRRRCVRVNPEAGRFGEDSSWMILAGK"
                     /name="MbeB family mobilization protein"
     gene            complement(<6367..6801)
                     /locus_tag="IN878_RS26065"
                     /pseudo=""
     CDS             complement(<6367..6801)
                     /locus_tag="IN878_RS26065"
                     /inference="COORDINATES: protein motif:HMM:NF015397.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(7010..7333)
                     /locus_tag="IN878_RS25540"
                     /old_locus_tag="IN878_25555"
     CDS             complement(7010..7333)
                     /locus_tag="IN878_RS25540"
                     /old_locus_tag="IN878_25555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_001347450.1"
                     /translation="MLTMWVTEDEHRRLLERCDGKQLAAWMRQTCLDEKPARAGKLPSL
                     SPALLRQLAGMGNNLNQIARQVNAGGGSGHDRVQVVAALMAIDAGLERLRHAVLEKGAD
                     DDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            complement(8580..9059)
                     /locus_tag="IN878_RS25545"
                     /old_locus_tag="IN878_25560"
     CDS             complement(8580..9059)
                     /locus_tag="IN878_RS25545"
                     /old_locus_tag="IN878_25560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001050742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001050742.1"
                     /translation="MNLLLPRDIVEAVLNDKKTKNARVAKCDGSEFFLELPSMNADFPA
                     GKIILKLGDSGFYNKRTKSLEGAYGLRHIWDKHRVEIGATSAEDIVIFLESILLAGAEV
                     LIDPKKGQNKAIVVESGTGMMILELKKPNGEDPYYSIITAYDRKSHPGTKLHTLI"
                     /name="hypothetical protein"
     gene            9319..10086
                     /locus_tag="IN878_RS25550"
                     /old_locus_tag="IN878_25565"
     CDS             9319..10086
                     /locus_tag="IN878_RS25550"
                     /old_locus_tag="IN878_25565"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_196042596.1"
                     /translation="MQPRELLMLSMYILVQAPVLTGLSKNQTESLTTNNKRGRRTAFYR
                     IKKHLQHDFYRIDSIKNEIHSPIKGFYMKDMKAVVVFTGKDLNIMRTEGGSGYWHARTD
                     RLNDADYLIAVRNRRETWAVKDLEHGTAFLIAKITGCFKSPDYDDRNVITFDEYAEIHT
                     PKAWKMLTDGQRYPVAYLSAQEAFLRIGVTPEQLEWKKFHPSSPSVPNTVIPGLAEEKT
                     EKLSLNEAIERAKKDISNATGIDSSAITISIKI"
                     /name="hypothetical protein"
     gene            complement(10285..10500)
                     /locus_tag="IN878_RS25555"
                     /old_locus_tag="IN878_25570"
     CDS             complement(10285..10500)
                     /locus_tag="IN878_RS25555"
                     /old_locus_tag="IN878_25570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_000137421.1"
                     /translation="MTELEKQLLSALEQLQQDYSKRLDEWENAFAEWRTMSGLMQRENA
                     ALSERVTGLSRQVQSLSEQLRRLSQG"
                     /name="MbeD family mobilization/exclusion protein"
     Typing          complement(2782..2954)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="98.276"
                     /coverage="100.0"
                     /evalue="9.21e-81"
     Typing          complement(7435..7621)
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="85.263"
                     /coverage="87.0"
                     /evalue="2.7100000000000003e-47"
     Typing          2879..3431
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="92.46"
                     /coverage="98.0"
                     /evalue="0.0"
     Typing          7601..8080
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="89.938"
                     /coverage="93.0"
                     /evalue="3.51e-174"
     Typing          complement(860..2355)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="90.982"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(2352..2668)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="93.396"
                     /coverage="100.0"
                     /evalue="2.51e-61"
     Typing          complement(5525..7020)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="90.782"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(10182..10567)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="92.248"
                     /coverage="100.0"
                     /evalue="5.88e-65"
ORIGIN
        1 ccatcatcac taacaatgct aatttcagtg tcgctcttga aaaaatgcct gctcaaaaca
       61 tcgctaaata aactatgttt ttttagatat atagacccac cgacatgccc tatgtaattg
      121 ccattcttat cataaatagg atgtgacaac aaaataatca aattcccagt aactgatttg
      181 aacggtgaag atatgaatgg tttcttttcc tcaatcgcca atttgctggc acttgaattc
      241 aagcgggtac caactaaatc aagagattct ggcgaagtcg ccagaacaac agcatcatta
      301 ctaacaacaa aaactgaatt aaacatacca gactgcaatc ttaacctatc cgcctcatct
      361 ttaagtgagt caatatcatt agttgatttt atttttcttg ctccatatgc taattcacct
      421 tgagccattt caatataacc actcattaca tctgacattt tcatagcgta cgatgaatta
      481 gcataaagca aatctttttc tatattttcg cgctgaaaaa ccataataac ggcaatcaga
      541 aaaatagaag ttaacaaaat cccaccaacg gacaaggtta tgactatcgg acgaaggtga
      601 ggctttttaa ttctcataaa aatacactcc atataataaa aaatgccgca tttaccataa
      661 catcccttat cagcaccgcc ccggacgggt tcggaacaaa tgcggcgtca gcccggtaaa
      721 accggcagca aactctccgc taaaccgcgc attctgcgcc agataaattt cacccggctg
      781 catggctatg cagtcgggtg aatggctccg gctttcagcg cagatgtccg ccgaaagccc
      841 cgttttatca ggttatcaca gtgaagggct gttatacgtc cgctgtcgct gagatcgctc
      901 cagctccagc gctttctgat gctgcaccac gcgctcctgc tcccggtgca gctcgacggc
      961 attcagtcgc tcgacaaccg gcgcagctgc tcgctcaaac tctgcacctg cctgctcaag
     1021 cccgtgacgc gctcgctcag cgctgcgttc tcccgttgca taagaccaga catcgtccgc
     1081 cattccgcga aggcgctctc ccactcgtcc agccttttcg agtagtcctg ctgtagctgc
     1141 tctaatgcgc tcagcaactg tttttccagc tccgtcatgt gctatttccc cgccagtatc
     1201 atccagctcg aatcctctcc gaaccgtccc gcttccgggt tcaccttcac gcagcggcgt
     1261 ctctgctcgc cgcaggtcga gagctgcacg ccgctgttcc tctctgacag catggcctgc
     1321 gtgctcttct gctcccggat ggtcgtgtag ttgtcgagta tctgctgccc ctgccaccac
     1381 agaatgcccg cgctcgaggc gatcagcagc gcggagacca ggacgatggt cagccacgtc
     1441 tggctgacca tgcgcaccat gcctttcgtg cgctggctca tggctgagga cagtttccgg
     1501 tcgtggtcga ggatggcggc gctgattctc ttcgcgcttt cgttcagttc cgcttttaca
     1561 gatttctcgt gctcgctgaa tgcggctttc agcatctcgc cggtattctg ctgctgcgct
     1621 ttcgatttct gctctaagtc cttcgccagc gttaaaagac tgttcataaa tggctccctt
     1681 cagtcggatg tttcgccccc cgtccgggtc ggcaatgctg atactgcttt ttgtggtgcg
     1741 gaccacctca aaacctgcgc tttccagcgc ctcagtgaca tcctgacggg ttttcagctc
     1801 cccggatgag gcaagagcaa gcagaccgca tgtaatcgct tctgcagcct cctgcttcgc
     1861 tttaggcagt gcagacggtg tgaccagtgc ccgccggttc tccggtgcgt tcgggtcgtg
     1921 cagccccagt ctgccgttca cgatggtctg ccaggcatcg atgcgtggac ggtcggcgcg
     1981 atcgtaatac ggctggagcc gtctgccggt cagcagctcc gtgttcggga tcagaaaatt
     2041 cagctcaagc cgccccttgt cacgatgctc cacccacagg atgctgtact ggtctttatc
     2101 aagtccgggc atcagaaccc gctcaaagct tgccatcagc ttttcccgct ggccggaagg
     2161 taaatccgct tccgcaaacg acagtacccc ggaggtgtat ttcttaacgt aaggcgacgc
     2221 gtcaatcagc tcccggactt cttccggctt cccctgcagg acggtggcgc cttcacgctg
     2281 ccggtcttta cccagcagat agtccaccgg cccggcgcca ccgccgcgac cgcgtggatg
     2341 aaatttaacg atcatcgtcc gttcctttct caagaacggc gtgccgcagc cgctcaagtc
     2401 cggcatcgat ggccatcagc gccgcaacaa tctgcaccct gtcatggccg gtaccgccgc
     2461 cggcgttaac acggcgggct atctggttca ggttattgcc catgccggca agctggcgca
     2521 gcagcgccgg cgagattgag ggaagctttc cggaacgtgc cggcttttcg tccaggcagg
     2581 tctgccgcat ccacgctgcc agctgcctgc cgtcgcagcg ctcaagcagc cgccggtgct
     2641 cgtcctccgt cacccacatg gtgagcatct tgttccgttt ctctgacaaa ccggcctcct
     2701 gataacgccg ggacgggcgg gaaagccttt cccggtcggc gcggggttgg acaagccgca
     2761 ggcgcgtcag gcttttggcg ggtttcgggg cgcagccctg aaccagtcac gtagcgctag
     2821 cggagtgtat actggcttac tatgttggca cggatgcgag tgtcagtgaa gtgcttcatg
     2881 tggcaggaga aaaatgcagc agcggagcgt cagcagaata tgtgatacag gatatattcc
     2941 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtaccggct
     3001 tacaggcggg gcggaaattt cctgggagat gccaggaaga agcttaacag ggaagcgata
     3061 aggccacggc gaagccgttt ttccataggc tccgcccccc tgacaagcat cacgaaatct
     3121 gacgctcaaa tcagtggtgg cgaaacccga caggactata aagataccag gcgtttcccc
     3181 ctggtggctc cctcgtgcgc tctcctgttc ctgcctttcg gtttaccgat gtcaatccgc
     3241 tgttatggcc gcgtttatct cattccacgc ctgacactcg gttccgggaa ggcagttcgc
     3301 tccaagctgg actgtatgca cgaacccccc gttcagcccg actaccacgt tgtcccggta
     3361 actatcatct tgagtccaac ccggaacaga cacgaaaaat cgccagtggc ggtagccatt
     3421 agtaagagga ttcacggagc ggagtagtag agtgttctac agagttcttg aagtggtggc
     3481 ctgactgcgg ctacactgga aggacagttt tggtacctgc gctccgtgaa agccagttac
     3541 cttcggaaga aagagttggt agctctgaat tccggcaaac gaaccaccgt tggtagcggt
     3601 ggtttttttg tttacagggt atgagattac gacgacatcg aagggataac aagaggatcc
     3661 gcttcttgcc cactcttcgc cacaaaaaaa acgacacact agcgcgtaaa gcatcatcaa
     3721 aaaactcaaa tagcgctata tacaatcact ttattacgcc cctccctttt ggcttgataa
     3781 agagcatcgt cagcattttt cagcaaaaaa tcgatcctac caagaggatc atcttgtgtt
     3841 gccacaccag cagatatggt gacccgcaag ttattaggga agacagtact ttcaataaca
     3901 ctcctaatac gctccgcaac acacttagct gttaccacag atgtattcgg caggaaaaca
     3961 acaaactctt cgccaccaaa ccgacaaaca acatcttcac tacggcaaca ggaattaata
     4021 atatgagcca aagaaacaag cacctcatct ccgaccccat gcccgaaact gtcatttatc
     4081 tctttaaaaa aatcaacatc aattacaata actgaatacc ctgaatcccg cttatgattc
     4141 tgtattttac ttaaaaaccc caatctattg ttcacgccgg taaggggatc tttaagcgcc
     4201 atctcattta aagaatcaac cctttccatc attaccttaa catgcgtaaa aagtgccttt
     4261 ttaagtcgat cagcttcggc ataccaagca ttgatagctt taatataaac taacgaagcc
     4321 cctatatctt tagcattggt agatatagca agcttttcta aaggaagtga aatacgggag
     4381 gcaatgaaat atgaaagaag tgaaaatgta ataattatta caattagcag aacaagtaca
     4441 cttttagcat aagtaataag aatagtagtc gcgctgtcag catggctata aaccaacaca
     4501 ttccaagcag gatttttcat atgagcataa cccagaagat atctattccc cgaagaaaaa
     4561 aatgccccct cacctctatc agactccgtt aactttgatt tcaaatcatc agacataacc
     4621 atgggccgac caacagcact gttatcgtta ttaaagatca cagttccatc atcactaaca
     4681 atgctaattt cagtgtcgct cttgaaaaaa tgcctgctca aaacatcgct aaataaacta
     4741 tgttttttta gatatataga cccaccgaca tgccctatgt aattgccatt cttatcataa
     4801 ataggatgtg acaacaaaat aatcaaattc ccagtaactg atttgaacgg tgaagatatg
     4861 aatggtttct tttcctcaat cgccaatttg ctggcacttg aattcaagcg ggtaccaact
     4921 aaatcaagag attctggcga agtcgccaga acaacagcat cattactaac aacaaaaact
     4981 gaattaaaca taccagactg caatcttaac ctatccgcct catctttaag tgagtcaata
     5041 tcattagttg attttatttt tcttgctcca tatgctaatt caccttgagc catttcaata
     5101 taaccactca ttacatctga cattttcata gcgtacgatg aattagcata aagcaaatct
     5161 ttttctatat tttcgcgctg aaaaaccata ataacggcaa tcagaaaaat agaagttaac
     5221 aaaatcccac caacggacaa ggttatgact atcggacgaa ggtgaggctt tttaattctc
     5281 ataaaaatac actccatata ataaaaaatg ccgcatttac cataacatcc cttatcagca
     5341 ccgccccgga cgggttcgga acaaatgcgg cgtcagcccg gtaaaaccgg cagcaaactc
     5401 tccgctaaac cgcgcattct gcgccagata aatttcaccc ggctgcatgg ctatgcagtc
     5461 gggtgaatgg ctccggcttt cagcgcagat gtccgccgaa agccccgttt tatcaggtta
     5521 tcacagtgaa gggctgttat acgtccgctg tcgctgagat cgctccagct ccagcgcttt
     5581 ctgatgctgc accacgcgct cctgctcccg gtgcagctcg acggcattca gtcgctcgac
     5641 aaccggcgca gctgctcgct caaactctgc acctgcctgc tcaagcccgt gacgcgctcg
     5701 ctcagcgctg cgttctcccg ttgcataaga ccagacatcg tccgccattc cgcgaaggcg
     5761 ctctcccact cgtccagcct tttcgagtag tcctgctgta gctgctctaa tgcgctcagc
     5821 aactgttttt ccagctccgt catgtgctat ttccccgcca gtatcatcca gctcgaatcc
     5881 tctccgaacc gtcccgcttc cgggttcacc ctcacgcagc ggcgtctctg ctctccgcag
     5941 gtcgagagct gcacaccgct gttcctctcg gacagcatgg cctgcgtgcg cttctgctcc
     6001 cggatggtcg tgtaattgtc gagtatctgc tgcccctgcc accacagaat acttgcgccc
     6061 gatgctgtca gcagtgtaga caccaagacg atggtcagcc acgtctggct gaccatccgc
     6121 agcatacctt tcgtgcgctg gctcatggca gcggacagct tcctgtcgtg gtcgaggatg
     6181 gcggcgctga ttctcttctc gctttcgctc agttccgctc tgacagactt ctcgtgctcg
     6241 ctgaatgcgg ctttcagcat ctcgccggta ctctgctgct gcgctttcga tttctgctct
     6301 aagtccttcg ccagcgttaa aagactgttc atagatggct cccttcagtc ggatgtttcg
     6361 ccccccgtcc gggtcggcaa tgctgatgct gctttttgtg gtgcgtacca cctcaaaacc
     6421 tgcgctttcc agcgcctcag tgacgtcctg gcgggttttc agctccccgg acgaggctaa
     6481 ggccagtaaa ccccgtgtaa ttgcctgagc ggcttcctgc ttcgtttccg gcagcgcgga
     6541 cggcgtgacc agcacccgcc ggttttcagg cgcgttcggg tcatgcagcc ccagcctgcc
     6601 gttcacgatg gtctgccagg catcaatgcg cggacggtcg gcgcggtcgt aatacggctg
     6661 gagacgcttg ccggtcagca gctccgtgtt cgggatcagg aagttcagct ccagccgccc
     6721 cttatcctgg tgctcaaccc acagcacgct gtactggtct ttatcgagtc cgggcatcag
     6781 aacccgttca aagctcgcca tcagcttttc gcgctgcccg ggcggtaaat ccttttcggc
     6841 aaaggacaag acgccggatg tgtacttttt tgcgtagggc gaggcgtcga taagctccct
     6901 gacttcttcc ggcttcccct gcagaacggt ggcgccgtcg cgctgtcggt ctttccccag
     6961 cagataatcc accggaccgg cgccaccgcc gcgccctcgc gggtgaaact taacgatcat
     7021 cgtctgcacc cttttccagc acggcatgcc gcagccgctc gagtccggca tctatggcca
     7081 tcagcgcggc gacgacctgc acgcggtcgt gcccgctgcc accaccggcg ttaacctgcc
     7141 gggcaatctg gttgaggttg ttccccatgc cggcaagctg acgaagcagc gccggcgaga
     7201 gcgagggaag cttgccggcg cgggcgggtt tctcgtccag gcacgtctgg cgcatccagg
     7261 ctgcaagctg tttaccgtcg cagcgctcca gcagacggcg gtgctcatct tcggtcaccc
     7321 acatcgtgag cattttgctg cgtttgtctg ccaatccggc ctcctgataa cgccgagacg
     7381 ggcgggaaat cttttcccgg tcggcgcggt gttggacaag ccgcaggcgc gtcagtggtt
     7441 ttagcgggtt tcggggcgca gccctgaacc agtcacgtag cgctagcgga gtgtatactg
     7501 gcttagtatg gtaaatcgaa gtggaaattc agggaagtgc ttcatgtggc aggtaaaaaa
     7561 ttcagctgtg gggcgtaagt gcgaaatgct ggagacgatg aaatgacgcc tcctcgctca
     7621 ctcggtcgct acgctcctgc cgtgagactg cggcgggcgt tacctgctca agaatgacga
     7681 gataaatagc gctcaaatca agaaggaagg ggaaaaggat ccggcgttgc cgtttttcca
     7741 taggctccgc ccccctgaca agcatcacga aatctgacgc tcaaatcagt ggtggcgaaa
     7801 cccgacagga ctataaagat accaggcgtt tccccctggt ggctccctcg tgcgctctcc
     7861 tgttcctgcc tttcggttta ccggtgtcat tccgctgtta tggccgcgtt tatctcattc
     7921 cacgcctgac actcagttcc gggtaggcag ttcgctccaa gctggactgt atgcacgaac
     7981 cccccgttca gtccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccga
     8041 aaagacacga cttaacgcca ctggcagcgg ccactggtaa gcaggatgtg cgagagatgt
     8101 atcgagagtt cttgaagtgg aggccttagt gaggctacac tggaaggaca gtatttggtg
     8161 acatgtctcg cacaagactg ttaccacggt tcagaaactt gccggtttac tgaaccttcg
     8221 aaaaaccacc ttgccgggtg gttttttcgt ttacagggag agagattacg acgagagtaa
     8281 aacgatcaca aggagatctg accgtttacc agtgatttgg cttcgttttg gtttccactg
     8341 aacaccaaaa tagcactttt tgcaaaaaga tgagcagtga tagagttgta ttctgtatgt
     8401 gtagaagggc ttggtatgta acctgaactc tggattggtt acatatttgt aggctacttg
     8461 tcggaaacca gatttcgaca atgcgttgtt aaggtcaaag acctgtcccg aaccgaaatg
     8521 ctaagggaat gccacgaaca acactattac gaagaagtcg gctatttagc cggctttttt
     8581 catattaggg tatgaagttt tgtacctgga tggctcttcc tatcataagc agtaatgatg
     8641 ctgtagtacg ggtcttcgcc gtttggtttt ttcaattcta gtatcatcat ccccgttcct
     8701 gactctacaa caatcgcttt gttttggcct tttttaggat ctatgagcac ttctgcccca
     8761 gctaataata tgctctccaa aaagatgaca atatcctcag cggaagtagc tcctatctcg
     8821 acacgatgct tgtcccatat atgcctaagg ccataggctc cctcgagact ttttgtcctt
     8881 ttgttgtaaa aaccggaatc tcctagcttg aggatgattt tccctgcggg aaagtctgca
     8941 ttcatgcttg gcagttctag aaaaaattca cttccatcac acttagccac tcgtgcattt
     9001 tttgttttct tatcattaag aacagcttca actatatcgc gtggaagtaa caagttcaca
     9061 aactacacct ctatagcctt cactattatg tttaggcgtt aaatcccttt ttgaagggct
     9121 gattctaaca ctgacgtaat gagcaatttc cagtaaatct tgatagaaaa aaccagttgt
     9181 atttaccata acattccttg cccgcaccgc tccggacggg ttcagcgtga aagcggcgtt
     9241 agcccggtaa aatcggcagt aaactccccg ctaaaccgcg cattctgcgc ctgataaatt
     9301 tcacgtggct gcatagccat gcagccacgt gaattgctca tgctttcaat gtatatcctc
     9361 gtccaagccc ctgttttaac cggtttatca aaaaaccaaa cagaaagcct taccactaac
     9421 aataaaagag gtaggaggac tgcattttat cgcataaaaa aacatttaca acacgatttt
     9481 tatcgtatag attccataaa aaatgaaatt cactcaccta ttaagggttt ctatatgaaa
     9541 gatatgaaag ctgttgtggt ttttaccggg aaggatttga acataatgag aactgagggg
     9601 gggtcaggct actggcatgc cagaacagac aggctcaacg atgccgatta cctcatagca
     9661 gtaagaaaca gaagggaaac gtgggcagta aaagacctgg aacacggcac agcattcctg
     9721 atcgccaaaa ttaccgggtg ttttaaaagc cctgattacg acgacaggaa cgtcataacc
     9781 tttgatgaat acgcagaaat tcacacgcca aaagcatgga aaatgctaac cgatggtcag
     9841 cgctatccgg ttgcttacct gagcgcacag gaagcttttt tacgtattgg ggtaacaccg
     9901 gaacaactgg aatggaagaa attccatcct tcatccccat cggttcctaa tacagtgatt
     9961 cctggtctgg ctgaagagaa aacagaaaaa ctctccctta acgaggcaat tgaaagagcc
    10021 aaaaaggata ttagcaacgc aacagggatt gatagctcag ccataacaat aagcatcaag
    10081 atctgatgaa gcgtgaacaa caagatagca gcctcatgtt ctagaactga acctgtacgc
    10141 cagaaacagc aagcccatgc ttttccggcg cacagattca aagtgacgga ccgttgtacg
    10201 ttcgctgtcg ctgagatcgt tccagctcca gcgctttctg atgctgcgcc gcccgttcct
    10261 gctcacgatg tgcctctatc acgttcagcc ttgtgacaac cggcgcagct gctcgctcaa
    10321 actctgcacc tgcctgctca agcccgtgac gcgctcgctc agcgccgcgt tctcccgttg
    10381 cataagacca gacatcgtcc gccattccgc gaaggcgttc tcccactcgt ccagcctttt
    10441 cgagtagtcc tgctgtagct gctctaatgc gctcagcaac tgtttttcca gctccgtcat
    10501 gtgctatttc cccgccagta tcatccagct cgaatcctct ccgaaccgtc ccgcttccgg
    10561 gttcacc
//
