LOCUS       NZ_CP063228             2493 bp    DNA     circular CON 22-APR-2024
DEFINITION  Enterobacter hormaechei subsp. steigerwaltii strain BD-50-Eh plasmid
            pBD-50-Eh_4, complete sequence.
ACCESSION   NZ_CP063228
VERSION     NZ_CP063228.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN16233514
            Assembly: GCF_015535675.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter hormaechei subsp. steigerwaltii
  ORGANISM  Enterobacter hormaechei subsp. steigerwaltii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1  (bases 1 to 2493)
  AUTHORS   Campos-Madueno,E.I., Gmuer,C., Risch,M., Bodmer,T. and Endimiani,A.
  TITLE     Characterisation of a new blaVIM-1-carrying IncN2 plasmid from an
            Enterobacter hormaechei subsp. steigerwaltii
  JOURNAL   J Glob Antimicrob Resist 24, 325-327 (2021)
   PUBMED   33571706
REFERENCE   2  (bases 1 to 2493)
  AUTHORS   Campos-Madueno,E.I., Bodmer,T. and Endimiani,A.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-OCT-2020) Institute for Infectious Diseases,
            University of Bern, Friedbuehlstrasse 51, Bern 3001, Switzerland
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 605.38x
            Sequencing Technology :: Oxford Nanopore MinION; Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_015535675.1-RS_2024_04_22
            Annotation Date                   :: 04/22/2024 04:51:24
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,181
            CDSs (total)                      :: 5,063
            Genes (coding)                    :: 4,935
            CDSs (with protein)               :: 4,935
            Genes (RNA)                       :: 118
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 46 of 128
            Pseudo Genes (incomplete)         :: 90 of 128
            Pseudo Genes (internal stop)      :: 16 of 128
            Pseudo Genes (multiple problems)  :: 21 of 128
            Pseudo Genes (short protein)      :: 1 of 128
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP063228.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2493
                     /organism="Enterobacter hormaechei subsp. steigerwaltii"
                     /mol_type="genomic DNA"
                     /strain="BD-50-Eh"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /sub_species="steigerwaltii"
                     /db_xref="taxon:299766"
                     /plasmid="pBD-50-Eh_4"
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.9321 N 7.4199 E"
                     /collection_date="2018-05-19"
                     /collected_by="Labormedizinisches Zentrum Dr Risch AG,
                     Waldeggstrasse 37, 3097 Liebefeld, Switzerland"
     gene            complement(join(2408..2493,1..133))
                     /locus_tag="IE977_RS25770"
                     /old_locus_tag="IE977_25770"
     CDS             complement(join(2408..2493,1..133))
                     /locus_tag="IE977_RS25770"
                     /old_locus_tag="IE977_25770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001046776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_198129632.1"
                     /translation="MFGFSSGALMTLSDDERHLLVSVVSVWLRRAGGDAGAMMLDAYRQ
                     ILSETEPAVRTVMLEFLESVRIHYISS"
                     /name="hypothetical protein"
     gene            1339..1566
                     /locus_tag="IE977_RS25775"
                     /old_locus_tag="IE977_25775"
     CDS             1339..1566
                     /locus_tag="IE977_RS25775"
                     /old_locus_tag="IE977_25775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010615788.1"
                     /GO_component="GO:0005886 - plasma membrane [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgdI/YgdR family lipoprotein"
                     /protein_id="WP_021243043.1"
                     /translation="MKKIFVSVFAAAVALSALTGCTRTSYAIHTNDGRTIVSDGKPKES
                     DSGLLGYTDANGVKQQINKTDVKEVSEIPH"
                     /name="YgdI/YgdR family lipoprotein"
     gene            1932..2117
                     /locus_tag="IE977_RS25780"
                     /old_locus_tag="IE977_25780"
     CDS             1932..2117
                     /locus_tag="IE977_RS25780"
                     /old_locus_tag="IE977_25780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_013772937.1"
                     /translation="MTRQESAALNMAKFIRAQTLLLLERLEQMDLDEAAGCCEHLHDQA
                     EALYAMLNAQIGEENA"
                     /name="Rop family plasmid primer RNA-binding protein"
     Typing          complement(2165..2252)
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="6.86e-39"
     Typing          396..924
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="88.598"
                     /coverage="88.0"
                     /evalue="0.0"
ORIGIN
        1 gccggtatgc atccagcatc atggctccgg catcccctcc ggcacgccgt agccagaccg
       61 aaacaacgga cacaagcagg tgtcgctcat catcactaag agtcatcagg gctccggaag
      121 aaaaaccaaa catggcttac ctactgttaa cggcaacgca gaagaaaggt ttacagggta
      181 gcgggcaggg atgaaaaaaa aaattgcagt caggccggaa cggctgctga accggtacag
      241 gcgggttccg ggcgcagcgc cggaccagtc acggagcact agcggagtgt atactggctt
      301 aagatgtagc atggcagtaa cacatagcat taatgccaca taagacaggc gcagaaagtg
      361 aaagaaaacg cgtcaggaga aaatgtgata cagaatatat tccgcttcct cgctcactga
      421 ctcgctccgc tcggtcgttc ggctgcggcg agcgggacgg gcttacggat ggatcagaaa
      481 tttcctgggg gatgccagga agatacttaa cgaagaaagc ataaggccgc gccaaagccg
      541 tttttccata ggctccgccc ccctgacaag catcacagaa tctgacgctc aaatcagtgg
      601 tggcgaaacc cgatgcggct taaagatacc aggcgtttcc ccctggtggc tccctcgtgc
      661 tcccccgcat tcctgccttt cggtttaccg gtgtcactcc gctgttatgg ccgcggtttc
      721 tcattccacg cctgacactc agttccggga aggcagttcg ctccaggatg gactgtatgc
      781 acgaaccccc cgttcagtcc gaccgctgcg ccttatccgg taactatcaa cttgagtcca
      841 acccggaaag acacgactaa tcgccattgg caacggccat tggcaaacag gatgtgcgag
      901 agatgtctca agagttcttg aagtggcggc cttagtgagg ctacactgaa aggacaaatt
      961 tggtgacatg tctcgcacaa gactgttacc acggttaggg gatctgccag gattacctaa
     1021 ccttcgaaaa accacctgcc agggtggttt tttcgtttac agggcaagag attacgcgcg
     1081 gaaaaaaagg atctcaagaa gatcctttat aaattagaca tccaacctca caaagaggaa
     1141 tacttaaatc atcttggtat atgtatcggc cggattacat ctcatataag cctcctcccc
     1201 gcctgacacc acactttgac catagcacct cgcgcctaac tcgatataca gcccaccatc
     1261 aagcctcttt tcacataaaa aatgacctat agttagatgg ttagtgacca aaactgatat
     1321 ggataaaagg agctggcttt gaaaaagatt tttgtttcag tgttcgcagc ggcagttgcg
     1381 ctttcagccc tcaccggctg cacgcgcaca agctatgcca tacacaccaa tgacggtcgc
     1441 actattgtca gcgacggcaa acctaaagaa tcagactcag gcctgcttgg ctatacagac
     1501 gcgaatggtg tcaaacagca gatcaataaa accgacgtta aagaagtctc cgaaattccg
     1561 cactgagcat cttctccgaa aaaggccgct aatgtggcct ttttctttac ctgtggttct
     1621 ccgccaaaat cccagcaaat tgcatcacca aagctaaaag ctttcagggt tgtctaaaaa
     1681 gcacaagaca taagaggaag tgcggtattt tataatcaag cccccaagaa tttaccataa
     1741 catccgttgc ccgcaccgcc tgagacgcgt tcagcgcgtt cctgacgaaa ccatgacaaa
     1801 aaccacaaca aaccaccccg gaacccgtca gaaacgcgcc tgttaaattt taacggcatg
     1861 catgactatg caccagaatg acgccatgct cttttcacgc aaaaatcatc accagacggg
     1921 gaaaatcacc agtgaccaga caggaatccg ccgccctcaa tatggccaaa tttatccgcg
     1981 cacagacact tctcctcctt gagcggctcg agcagatgga tctggatgag gctgccggct
     2041 gctgtgagca cctgcacgat caggccgaag cgctttacgc catgctgaac gcacagatag
     2101 gcgaggaaaa tgcgtgaaaa tcggtgaacg ggtgcgcaat tcagtgcgcg gccgtgaggc
     2161 gatggcgggg tgtcggggcg cagccctgac cagggtattt gtgatgccgg cgcgtgcgcg
     2221 gtattacaaa tgcacatcct gtcccggaac ggacaccggg aaacagcaaa aaaaaccggg
     2281 cggcacgccc ggaactcaat caagttagat tagattactc tcactcgtcc ataacagcat
     2341 catggaacga cgaccaccgt ccgtgacggc cgcctcgttt aagtatggac agaaatacag
     2401 aaaatgctca ggacgaaatg taatgaatgc gaacggattc aagaaattcg agcatgacag
     2461 tccttacggc cggttcggtt tcagacaaaa tct
//
