LOCUS       NZ_CP063022             3511 bp    DNA     linear   CON 15-MAY-2024
DEFINITION  Klebsiella pneumoniae strain CriePir75 plasmid unnamed3, complete
            sequence.
ACCESSION   NZ_CP063022
VERSION     NZ_CP063022.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN13153741
            Assembly: GCF_009661535.2
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 3511)
  AUTHORS   Shelenkov,A., Mikhaylova,Y., Yanushevich,Y., Samoilov,A.,
            Petrova,L., Fomina,V., Gusarov,V., Zamyatin,M., Shagin,D. and
            Akimkin,V.
  TITLE     Molecular Typing, Characterization of Antimicrobial Resistance,
            Virulence Profiling and Analysis of Whole-Genome Sequence of
            Clinical Klebsiella pneumoniae Isolates
  JOURNAL   Antibiotics (Basel) 9 (5), 261 (2020)
   PUBMED   32429555
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3511)
  AUTHORS   Shelenkov,A., Mikhaylova,Y., Yanushevich,Y., Petrova,L., Fomina,V.,
            Zamyatin,M. and Shagin,D.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-SEP-2020) Department Of Molecular Diagnostics and
            Epidemiology, Central Research Institute of Epidemiology,
            Novogireevskaya str., 3a, Moscow 111123, Russia
REFERENCE   3  (bases 1 to 3511)
  AUTHORS   Shelenkov,A., Mikhaylova,Y., Yanushevich,Y., Samoilov,A.,
            Petrova,L., Fomina,V., Gusarov,V., Zamyatin,M. and Shagin,D.
  TITLE     Direct Submission
  JOURNAL   Submitted (31-OCT-2019) Department Of Molecular Diagnostics and
            Epidemiology, Central Research Institute of Epidemiology,
            Novogireevskaya str., 3a, Moscow 111123, Russia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.8-beta
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 64x
            Sequencing Technology             :: Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_009661535.2-RS_2024_05_15
            Annotation Date                   :: 05/15/2024 02:55:25
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,555
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,321
            CDSs (with protein)               :: 5,321
            Genes (RNA)                       :: 124
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 110
            CDSs (without protein)            :: 110
            Pseudo Genes (ambiguous residues) :: 0 of 110
            Pseudo Genes (frameshifted)       :: 40 of 110
            Pseudo Genes (incomplete)         :: 63 of 110
            Pseudo Genes (internal stop)      :: 21 of 110
            Pseudo Genes (multiple problems)  :: 12 of 110
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP063022.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3511
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="CriePir75"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="unnamed3"
                     /geo_loc_name="Russia: Moscow"
                     /lat_lon="55.76 N 37.62 E"
                     /collection_date="2017"
                     /collected_by="Pir Nat Surg Centre"
     gene            complement(<1..634)
                     /locus_tag="GJJ10_RS27620"
                     /old_locus_tag="GJJ10_027620"
     CDS             complement(<1..634)
                     /locus_tag="GJJ10_RS27620"
                     /old_locus_tag="GJJ10_027620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000635827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="WP_193699470.1"
                     /translation="MIVKFHPRGRGGGAGPVDYLLGKDRQREGASVLQGKPEEVRELID
                     ASPYVKKYTSGVLSFAEADLPPGQREKLMASFERVLMPGLDKDQYSILWVEHADKGRLE
                     LNFLIPNTELLTGKRLQPYYDRADRPRIDAWQTVVNGRLGLHDPNDPENRRALVTPSGL
                     PETKQEAAETITRGLLMLASSGELKTRDDVTGALTAAGFEVVRTTKNS"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(624..965)
                     /locus_tag="GJJ10_RS27625"
                     /old_locus_tag="GJJ10_027625"
     CDS             complement(624..965)
                     /locus_tag="GJJ10_RS27625"
                     /old_locus_tag="GJJ10_027625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006122514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_071599364.1"
                     /translation="MADKRNKMLTMWVTEDEHRRLLERCDGKQLAAWMRQTCLDEKPTR
                     AGRLPSISPALLRQLAGMGNNLNQIARKVNAGGAGHDRVQIVAALMAIDAGLERLRHAV
                     LEKGPDDDC"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            complement(2161..2343)
                     /locus_tag="GJJ10_RS27630"
                     /old_locus_tag="GJJ10_027630"
     CDS             complement(2161..2343)
                     /locus_tag="GJJ10_RS27630"
                     /old_locus_tag="GJJ10_027630"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_016265984.1"
                     /translation="MLTFIGVVVVAVIAFKLFMLFFSSSESAVERATRKYMENPTSANY
                     KIMVATRMRNEKKRG"
                     /name="hypothetical protein"
     gene            complement(2738..2953)
                     /locus_tag="GJJ10_RS27635"
                     /old_locus_tag="GJJ10_027635"
     CDS             complement(2738..2953)
                     /locus_tag="GJJ10_RS27635"
                     /old_locus_tag="GJJ10_027635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015328239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_015328239.1"
                     /translation="MTELEKQLLSALEQLQQDYSRRLDEWESAFEEWRKMSGLMQRENA
                     ALSERVSGLSRQVQSLSEQLRRLSTG"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(2960..3457)
                     /locus_tag="GJJ10_RS27640"
                     /old_locus_tag="GJJ10_027640"
     CDS             complement(2960..3457)
                     /locus_tag="GJJ10_RS27640"
                     /old_locus_tag="GJJ10_027640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020325392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_015328238.1"
                     /translation="MSSLLTASRDFEEKSKAQQQLTEERLKAAFSKHENVVKSELNASA
                     KRINDAISAHEQGMNAAMQSNRLSVLRMVGRTWLTITMVSGLLFASLSGVLWYQGNRIA
                     ANLAEIRQQRDTLSKLHMQTWGVTYLENRNGRFLVLPEGMKAETGRTVDNKTRNAVKLV
                     RE"
                     /name="MbeB family mobilization protein"
     Typing          complement(1051..1224)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="2.5600000000000002e-86"
     Typing          1191..1712
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="93.69"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          complement(3..634)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="99.0"
                     /evalue="2.13e-138"
     Typing          complement(631..944)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="92.453"
                     /coverage="99.0"
                     /evalue="9.860000000000001e-59"
     Typing          complement(2623..3509)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 tactgttttt tgtggttctg accacctcaa aaccggccgc tgtgagcgcc cctgtgacgt
       61 catcacgcgt tttcagctct cctgatgagg ctaacatcag caaaccccgt gtaatcgtct
      121 ctgcggcttc ctgcttcgtt tctggcagtc ctgaaggcgt gaccagcgcc cgccggttct
      181 ccgggtcgtt cggatcatgt agccccagcc tgccgttgac cactgtctgc cacgcatcga
      241 tgcgcggccg gtcggcacga tcgtaatacg gctggaggcg tttccccgtc agtaattcgg
      301 tattggggat cagaaaattc agctcaagac gacccttgtc tgcgtgttcc acccacagaa
      361 tgctgtactg gtctttatcg agtccgggca tcagaacccg ctcaaagctg gccatcagtt
      421 tttcacgctg acccggcggc aggtccgcct ccgcgaatga cagcaccccg gaggtatatt
      481 ttttcacata cggcgacgca tcaatcagct cccggacttc ttccggcttc ccctgcagga
      541 cgctggcacc ttcacgctgc cggtctttac ccagaagata atccacagga ccggcgcccc
      601 cgccgcgacc ccggggatgg aatttaacaa tcatcgtccg gccctttttc cagtacggca
      661 tgccgcaacc gttccagacc ggcatcgatc gccatcagcg ctgccacgat ctgcacccgg
      721 tcatgaccgg caccaccggc gttcactttc cgggcgatct ggttcaggtt attgcccatg
      781 ccggcaagct gacgcagcag cgcgggcgaa atcgagggaa gcctgccggc gcgtgtcggt
      841 ttctcatcaa ggcaggtctg gcgcatccac gccgccagtt gttttccgtc gcaccgctca
      901 agcaaccgcc ggtgttcgtc ttccgtcacc cacatcgtga gcatcttgtt gcgtttatct
      961 gccagaatcg cctcctgata acgccgtgac ggacgggaaa gcctttcccg gtcggcgcgg
     1021 ggttggacaa gccgcaggcg cgtcagtggg gtttcggggc gcagccctga accagtcacg
     1081 tagcgctagc ggagtgtata ctggcttact atgttggcac ggatgaggat gtcagtgaag
     1141 tgcttcatgt ggcaggagaa aaaaggcggc accggcgcgt cagcagaata tgtgatacag
     1201 gatatattcc gcttcctcgc tcactgactc gctacgctcg gtcgttcgac tgcggcgagc
     1261 ggaaatggct tacgaacggg gcggagattt cctggaagat gccaggaaga tacttaacag
     1321 ggaagcgata gggccgcggc gaagccgttt ttccataggc tccgcccccc tgacaagcat
     1381 cacgaaatct gacgctcaaa tcagtggtgg cgaaacccga caggactata aagataccag
     1441 gcgtttcccc ctggcggctc cctcgtgcgc tctcctgttc ctgcctttcg gtttaccggt
     1501 gtcattccgc tgttatggcc gcgtttatct cattccacgc ctgacactca gttccgggta
     1561 ggcagttcgc tccaagctgg actgtatgca caaacccccc gttcagtccg actgctgcgc
     1621 cttacccggt aactatcatc ttgagtccaa cccgagaaag cacgacaaag cgccactggc
     1681 agcagccact ggtaactggg atatacgaga gagagattca gaggtcttga agtatgggcc
     1741 tgactgcggc tacactgaaa gaacagtttt ggtatctgcg gtctcgtata cccagttacc
     1801 tggttaagca gttccccaac tgacttaacc ttcgatcaaa ccacctcccc aggtggtttt
     1861 ttcgttttca gagcaagaga ttacgactga aacgacagga tcacaagaag atcattaact
     1921 ccgagagtag taactcccac catgtttgcc tatcaacaca cttaggaaac attacttgcg
     1981 tttagttata cgacttctag ctctccataa cagatcctca ttttctgctg atggatttcg
     2041 acggtatcgt tcctccgctt tccgtactcc atattcagga aacaccctga cagcaaccat
     2101 ttgtataaag acataacaca ccaaaagtaa aaataaaaaa ccaataaact caaacatata
     2161 tcaccctctt ttcttctcat ttctcatgcg tgtagccacc attattttat agttagcact
     2221 cgtagggttc tccatatatt tacgggtagc acgttcgacg gcagattcgg atgaactaaa
     2281 aaaaagcata aataatttaa atgcaataac ggcgactacc actacaccaa taaatgtaag
     2341 catcactctt actcctgtta tcaacattaa acataacatc tcttacccgc accgcgccgg
     2401 aggcactcag ggcgtttctg atggctgacg ggcaaaaggt ggtggcaagt acaccgaaaa
     2461 ccaccagaaa cgcacctgat ttattttacg catatgcatc acttattcac atcactgcat
     2521 agccggtgat ttcggttaaa aaagactctc tgtgtgctga aatcgcagaa aatggccatg
     2581 atccccgtca cgctcaggcc acctgagacg ggcgtttatc agtctccata ctccctgtca
     2641 ccatgagata atgtgcgttg ccgttccggt accagatctg ctgtcttctc tggttccttt
     2701 tccgttgttt gcccgtatcg ctgttgctca atctcattca gcctgtcgat aaccggcgca
     2761 gctgctcgct caaactctgc acctgcctgc tcaagcccga cacgcgctcg ctcagcgccg
     2821 cgttctcccg ttgcataaga ccagacatct tccgccattc ctcgaaggcg ctctcccact
     2881 cgtccagcct tctcgagtag tcctgctgta gctgctctaa cgcgctcagc aattgttttt
     2941 ccagttccgt cattctgctc tactcccgta ccagcttcac cgcgttccgc gtcttgttgt
     3001 ccaccgtccg gcccgtttcg gctttcatcc cctccggcag caccagaaac cgtccgttgc
     3061 ggttctccag ataggtcacg ccccacgtct gcatgtgcag tttcgacagc gtatctctct
     3121 gctgcctgat ttccgccagg ttcgccgcta tccggttccc ctggtaccac agcaccccgc
     3181 tcaggctggc gaacagcagc cctgacacca tggtgatggt cagccacgtc cgcccgacca
     3241 ttcgcagcac gctcagccgg ttcgactgca tcgccgcgtt catcccctgt tcgtgggcgc
     3301 tgatggcgtc gttgattctc ttcgcgcttg cgttcagttc cgatttgaca acgttctcgt
     3361 gcttgctgaa tgcggctttc agcctctctt cggtaagctg ctgctgtgct ttcgatttct
     3421 cctcgaagtc cctcgaggcc gttaaaagac tgctcataaa tggctcccct cagtcggata
     3481 tttcgccccc cgtccgggtc ggcaatgctg a
//
