LOCUS       NZ_CP063006             4052 bp    DNA     linear   CON 16-MAY-2024
DEFINITION  Klebsiella pneumoniae strain CriePir154 plasmid unnamed3.
ACCESSION   NZ_CP063006
VERSION     NZ_CP063006.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN13153753
            Assembly: GCF_009661455.2
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 4052)
  AUTHORS   Shelenkov,A., Mikhaylova,Y., Yanushevich,Y., Samoilov,A.,
            Petrova,L., Fomina,V., Gusarov,V., Zamyatin,M., Shagin,D. and
            Akimkin,V.
  TITLE     Molecular Typing, Characterization of Antimicrobial Resistance,
            Virulence Profiling and Analysis of Whole-Genome Sequence of
            Clinical Klebsiella pneumoniae Isolates
  JOURNAL   Antibiotics (Basel) 9 (5), 261 (2020)
   PUBMED   32429555
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4052)
  AUTHORS   Shelenkov,A., Mikhaylova,Y., Yanushevich,Y., Petrova,L., Fomina,V.,
            Zamyatin,M. and Shagin,D.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-SEP-2020) Department Of Molecular Diagnostics and
            Epidemiology, Central Research Institute of Epidemiology,
            Novogireevskaya str., 3a, Moscow 111123, Russia
REFERENCE   3  (bases 1 to 4052)
  AUTHORS   Shelenkov,A., Mikhaylova,Y., Yanushevich,Y., Samoilov,A.,
            Petrova,L., Fomina,V., Gusarov,V., Zamyatin,M. and Shagin,D.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) Department Of Molecular Diagnostics and
            Epidemiology, Central Research Institute of Epidemiology,
            Novogireevskaya str., 3a, Moscow 111123, Russia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.8-beta
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 26x
            Sequencing Technology             :: Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_009661455.2-RS_2024_05_16
            Annotation Date                   :: 05/16/2024 03:16:21
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,273
            CDSs (total)                      :: 5,148
            Genes (coding)                    :: 5,020
            CDSs (with protein)               :: 5,020
            Genes (RNA)                       :: 125
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 13
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 69 of 128
            Pseudo Genes (incomplete)         :: 55 of 128
            Pseudo Genes (internal stop)      :: 13 of 128
            Pseudo Genes (multiple problems)  :: 9 of 128
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP063006.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..4052
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="CriePir154"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="unnamed3"
                     /geo_loc_name="Russia: Moscow"
                     /lat_lon="55.76 N 37.62 E"
                     /collection_date="2017"
                     /collected_by="Pir Nat Surg Centre"
     gene            complement(392..604)
                     /locus_tag="GJI94_RS26225"
                     /old_locus_tag="GJI94_026225"
     CDS             complement(392..604)
                     /locus_tag="GJI94_RS26225"
                     /old_locus_tag="GJI94_026225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_000137423.1"
                     /translation="MTELEKQLLTALEQLHQDYSQRLDEWESAFAEWQKMSGLMQRENA
                     ALNERVTRLSQQVANLSRQLQRLSQ"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(608..1093)
                     /locus_tag="GJI94_RS26230"
                     /old_locus_tag="GJI94_026230"
     CDS             complement(608..1093)
                     /locus_tag="GJI94_RS26230"
                     /old_locus_tag="GJI94_026230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001749519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_055314654.1"
                     /translation="MSSLLALAKDLEQQSKAQQQSTGEMLKAAFSEHEKSVKAELSASA
                     KRISDAISAHEQGMTAAMQSNRLSVLRMVGRTWLTITLVSVLLIAMSGSILWWQGQQIT
                     GNYQTIRAQERTQAMLSEKNHGVQLSLCGEKKLSCVKVNPKAGAYGEEGNWMVLERK"
                     /name="MbeB family mobilization protein"
     gene            complement(<1131..1808)
                     /locus_tag="GJI94_RS26660"
                     /old_locus_tag="GJI94_026235"
                     /pseudo=""
     CDS             complement(<1131..1808)
                     /locus_tag="GJI94_RS26660"
                     /old_locus_tag="GJI94_026235"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(1771..2112)
                     /locus_tag="GJI94_RS26240"
                     /old_locus_tag="GJI94_026240"
     CDS             complement(1771..2112)
                     /locus_tag="GJI94_RS26240"
                     /old_locus_tag="GJI94_026240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006122514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_057172823.1"
                     /translation="MADKRNKMLTMWVTEDEHRRLLERCDGRQLAAWMRQSCLDEKPAR
                     ACRLPSISPALLRQLAGMGNNLNQIARKVNAVGAGHDRVQIVAALMAIDAGLERLRHAV
                     LEKGGDDDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            3478..3936
                     /locus_tag="GJI94_RS26245"
                     /old_locus_tag="GJI94_026245"
     CDS             3478..3936
                     /locus_tag="GJI94_RS26245"
                     /old_locus_tag="GJI94_026245"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_016245712.1"
                     /translation="MIETTLLAVLTGALSNLTSDAIKGIIGKLASKHPEVEQQASLLET
                     PLPFDEVDKLFSQMQGAIQAAAANGEIQLDGAELKALSGIKFDHQAGTVLINGSQVQAS
                     ILVTGGGQHATGKTIIQGDTTMTSQGTSIKMSGGAQIVMTGGARIIQN"
                     /name="hypothetical protein"
     Typing          complement(2200..2379)
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="93.407"
                     /coverage="83.0"
                     /evalue="1.57e-69"
     Typing          2356..2842
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="88.235"
                     /coverage="93.0"
                     /evalue="5.96e-162"
     Typing          complement(277..1781)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="94.223"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 gataaatttt acccggctgc atggtcatgc agccagatgc atacggtctg atttcaggcc
       61 agaatctgga tgaacgcctt aaaaacagga aaatgcttct ccagccagtc ccagatctcc
      121 agcgccatgt acaggcagtt cagcaccgtc tcaatcacgt taaacagtct cataatccct
      181 ccgttgtata cgccgtgatg ttttcatcac gaccgtattt agcgggaaat ttatgagccc
      241 ttcttcgtgg ggtttgtcac tggtgaagcc tgtcagccca gcgtcggacc gttatagctc
      301 cgctgacggg aacgggcttc cgccagctct ttttcgtgct gtcgctgctc gtatgcccgt
      361 tcggcggcca ccgccatttc atgccgctgc actactggct caaacgctgt aactgcctgc
      421 tcaagttcgc cacctgctga ctcaagcgcg tgacgcgctc gttcagcgcc gcgttctccc
      481 gttgcataag accagacatc ttctgccatt ccgcgaaggc gctctcccac tcgtccagcc
      541 tttgcgagta gtcctgatgt agctgctcta atgcggtcag caactgtttt tccagttccg
      601 tcatgggcta tttcctctcc agtaccatcc agttcccttc ctccccgtaa gctcccgctt
      661 tcgggttcac tttgacgcag gacagtttct tctccccgca gagcgacagc tgaacgccgt
      721 ggtttttctc cgacaacatg gcctgcgtcc gctcctgcgc ccggatagtc tggtaattcc
      781 cggtgatctg ctgcccctgc caccacagaa tgctcccgct catggcgatc agcagtaccg
      841 agaccagggt gatggtcagc cacgtccggc cgaccatacg cagcacgctc aggcggttcg
      901 actgcatcgc cgcggtcata ccctgctcgt gggcgctgat ggcgtcgctg attctcttcg
      961 cgcttgcgct cagttccgct tttacagatt tctcgtgctc gctgaatgcg gctttcagca
     1021 tctcgccggt actctgctgc tgcgctttcg attgctgctc caagtccttc gccagcgcta
     1081 aaagactgct cataaatggc tcccttcagt cggatgtttc gccccccgtc cgggtcggca
     1141 atgctgatac tgttttttgt agtgcggacc acctcaaaac ctgccgctgt gagcgcacct
     1201 gtgacgtcct cgcgagtttt gagctccccg gatgaggcta aggccagcaa cccacgtgta
     1261 atcgcctgag cggcttccag cttcgtttcc ggcagtgcgg acggcgtaac cagtaaccgc
     1321 cggttctccg gggcgtttgg atcatgcagt cccagtctgc cgttcaccac ggtctgccag
     1381 gcatcgatgc gcggccggtc ggcgcgatcg taatacggct gcaggcgttt cccggtcagc
     1441 agttcagtgt tggggatcag gaaattcagc tccagccgcc ccttgtcggt gtgctccacc
     1501 cacagaatgc tgtactggtc tttatcgagc ccgggcatca gaacccgctc aaagctggcc
     1561 atgatctgct cccgctgccc gggcagcagt tcagcctccg cgaacgacag cacgccggag
     1621 gtgtactttt tcgcatacgg cgaggcatcg ataagctccc ggacttcctc cggttttccc
     1681 tgcagcacgg tcgccccctc gcgctgccgg tcttttccca gcaggtaatc caccggaccg
     1741 gcaccaccac cgcgcccccg gggatgaaat ttaacgatca tcatcccccc ctttttccag
     1801 caccgcatgc cgcagccgct ccagtcccgc gtctattgcc atcagggcgg ccacaatctg
     1861 cacccgatca tgaccggcac caacggcatt cactttccgg gcgatctggt tcaggttgtt
     1921 acccataccg gcaagctggc gcagcagcgc cggcgatatt gacggcagtc tgcaggcacg
     1981 cgccggcttc tcatcgaggc aggactgacg catccacgcc gccagctgcc tgccgtcgca
     2041 ccgctcgagc agccgccggt gttcgtcttc cgtcacccac atcgtgagca tcttgttgcg
     2101 tttgtctgcc agagtagcct cctgataaca ccgggacggg cgggaaatgt ttcccggtcg
     2161 gcacggtgtt ggacaagccg caggcgcgtc agtggctttt agcgggtttc ggggcgcagc
     2221 cctgaaccag tcacgtagcg ctagcggagt gtatactggc taaatatgtt acggcggagt
     2281 ggtaatgtat gaaagtgctt catgtgggaa ggggaaaatg tggctgacgg gcgtaagtgc
     2341 aacatgctgg taaaggtgaa atgacgcctc ctcgctcact cagtcgctgc gctcctgccg
     2401 tgagactgcg gcgggcgtta ccagctcaca gatgacggga tacgcaagca gcgctcaaat
     2461 caggagaagc cgggaagagg atgcggcgtt gccgtttttc cataggctcc gcccccctga
     2521 cgagtatcat aaaatctgac gctcaaatca gtggtggcga aacccgacag gactataaag
     2581 atcccaggcg tttccccctg gtagctccct cgtgcgctct cctgttcctg ccattcggtt
     2641 taccggtgtc attccgctgt tatggccgcg tttgtctcat tccacgcctg acactcagtt
     2701 ccgggtaggc agttcgctcc aagctggact gtatgcacga tccccccgtt cagtccgact
     2761 accacgctct tttcggtaac tatcgtcttg agtccaaccc gaaaaggcac gacaaatcgc
     2821 cattggcagt agccactggt aaacaggatg tgcgagagat gtattcagag ttcttgaagt
     2881 ggtggcctta ctgcggctac actggaagga cagctttggt gacatgtctc gcacaagact
     2941 gttaccacgg ttagtaaatc tgccaggatt aactaacctt cgaaaaacca cctgccaggg
     3001 tggttttttc gtttacagag caaaagacta cgctcagaaa gagattatct caagaagatc
     3061 atctctttct cttactcaag tcaaagcaaa aaaacaccac tccatcacaa actcaggtta
     3121 tagtcggtat gcaactaata acactgtaat ttctgtgcaa aatcgaggtt atgcatatgc
     3181 aaatcaacat caactgcaag agtatcccaa catctttcga tgttgtctgc tcgaattgtg
     3241 aatggaccgg cgtggctaca ttagattatc cggaaaacgt aaaaacccta tctgtcggag
     3301 gagaaataaa tttcactcat aatgacgaga cgccaaaatg tccacaatgt agcgttggat
     3361 atatatatgg taaaagtggc acttataaac gtaatagtgc aactaaccat atggacagaa
     3421 tcggagatta taaagagtaa ataatacgca tcacagcaat ctaacgaggg aaatataatg
     3481 attgaaacca cactacttgc cgttctcact ggcgcattaa gcaatcttac ttctgatgca
     3541 atcaaaggca tcatagggaa gttagcaagt aaacatcctg aagttgaaca acaagcctca
     3601 ctgcttgaaa caccattacc ttttgacgaa gttgataaat tattttctca gatgcagggg
     3661 gccattcagg ctgcagcagc caatggtgaa attcagctag atggagctga attaaaagca
     3721 ttgagcggaa taaaatttga tcaccaagcc ggaactgttt tgataaatgg atctcaagta
     3781 caagcgtcaa ttttagtcac tggtggaggt caacatgcta ctggaaaaac gatcatacaa
     3841 ggtgatacga caatgactag ccagggtaca agtataaaaa tgtcaggtgg tgctcaaata
     3901 gtaatgactg gtggagcccg aatcattcag aactaatatg atttaccata acatctcttg
     3961 cccgcaccgc tccggacatg ttcgtcacaa acggggcggc agtcaggtaa aaccggcaac
     4021 aaaccacccg atacaccgcc ccgttcgtga ca
//
