LOCUS       NZ_CP063002             3223 bp    DNA     linear   CON 08-MAY-2024
DEFINITION  Klebsiella pneumoniae strain CriePir197 plasmid unnamed5.
ACCESSION   NZ_CP063002
VERSION     NZ_CP063002.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN13153760
            Assembly: GCF_009661415.2
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 3223)
  AUTHORS   Shelenkov,A., Mikhaylova,Y., Yanushevich,Y., Samoilov,A.,
            Petrova,L., Fomina,V., Gusarov,V., Zamyatin,M., Shagin,D. and
            Akimkin,V.
  TITLE     Molecular Typing, Characterization of Antimicrobial Resistance,
            Virulence Profiling and Analysis of Whole-Genome Sequence of
            Clinical Klebsiella pneumoniae Isolates
  JOURNAL   Antibiotics (Basel) 9 (5), 261 (2020)
   PUBMED   32429555
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3223)
  AUTHORS   Shelenkov,A., Mikhaylova,Y., Yanushevich,Y., Petrova,L., Fomina,V.,
            Zamyatin,M. and Shagin,D.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-SEP-2020) Department Of Molecular Diagnostics and
            Epidemiology, Central Research Institute of Epidemiology,
            Novogireevskaya str., 3a, Moscow 111123, Russia
REFERENCE   3  (bases 1 to 3223)
  AUTHORS   Shelenkov,A., Mikhaylova,Y., Yanushevich,Y., Samoilov,A.,
            Petrova,L., Fomina,V., Gusarov,V., Zamyatin,M. and Shagin,D.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-OCT-2019) Department Of Molecular Diagnostics and
            Epidemiology, Central Research Institute of Epidemiology,
            Novogireevskaya str., 3a, Moscow 111123, Russia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.8-beta
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 214x
            Sequencing Technology             :: Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_009661415.2-RS_2024_05_08
            Annotation Date                   :: 05/08/2024 05:36:25
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,441
            CDSs (total)                      :: 5,324
            Genes (coding)                    :: 5,156
            CDSs (with protein)               :: 5,156
            Genes (RNA)                       :: 117
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 62 of 168
            Pseudo Genes (incomplete)         :: 110 of 168
            Pseudo Genes (internal stop)      :: 19 of 168
            Pseudo Genes (multiple problems)  :: 20 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP063002.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..3223
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="CriePir197"
                     /isolation_source="Gall bladder"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="unnamed5"
                     /geo_loc_name="Russia: Moscow"
                     /lat_lon="55.76 N 37.62 E"
                     /collection_date="2018"
                     /collected_by="Pir Nat Surg Centre"
     gene            195..518
                     /locus_tag="GJJ12_RS27060"
                     /old_locus_tag="GJJ12_027060"
     CDS             195..518
                     /locus_tag="GJJ12_RS27060"
                     /old_locus_tag="GJJ12_027060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_004193914.1"
                     /translation="MLTMWVTEDEHRRLLERCEGKQLAAWMRQTCLDEKPARAGKLPSI
                     SPALLRQLAGMGNNLNQIARQVNAGGGSGHDRVQIVAALMAIDAGLERLRHAVLEKGAD
                     DDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            727..>1161
                     /locus_tag="GJJ12_RS27370"
                     /pseudo=""
     CDS             727..>1161
                     /locus_tag="GJJ12_RS27370"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            1196..1681
                     /locus_tag="GJJ12_RS27070"
                     /old_locus_tag="GJJ12_027070"
     CDS             1196..1681
                     /locus_tag="GJJ12_RS27070"
                     /old_locus_tag="GJJ12_027070"
                     /inference="COORDINATES: protein motif:HMM:NF016710.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_025367738.1"
                     /translation="MNSLLTLAKDLEQKSKAQQQTTGEMLKAAFSEHEKSVRAELSESE
                     KRISAAILDHDRKLSSAMSQRTKGMLRMVSQTWLTIVLVSALLIASSAGILWWQGQQIL
                     ENYTTIREQKSTQAMLSERNSGVQLSTCGEQRRRCVRVNPEAGQFGEDSSWMILAGK"
                     /name="MbeB family mobilization protein"
     gene            1685..1900
                     /locus_tag="GJJ12_RS27075"
                     /old_locus_tag="GJJ12_027075"
     CDS             1685..1900
                     /locus_tag="GJJ12_RS27075"
                     /old_locus_tag="GJJ12_027075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_024195375.1"
                     /translation="MTELEKQLLSALEQLQQDYSKRLDEWENAFAEWRTMSGLIQRENA
                     ALNERVTVLSRQVQSLSEQLRRLSKG"
                     /name="MbeD family mobilization/exclusion protein"
     gene            2068..2400
                     /locus_tag="GJJ12_RS27080"
                     /old_locus_tag="GJJ12_027080"
     CDS             2068..2400
                     /locus_tag="GJJ12_RS27080"
                     /old_locus_tag="GJJ12_027080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008784643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EmrE family multidrug efflux SMR transporter"
                     /protein_id="WP_001099531.1"
                     /translation="MNTYIFLGGAIVAEVIGTTFMKYSEGFTRLWPSVGTAISYCAAFY
                     LLSQTLENIPTGIAYAIWSGAGIVLISLAGWLVSGQKLDWPAIAGMFLICVGVLVINIF
                     SKSSAH"
                     /name="EmrE family multidrug efflux SMR transporter"
     ARG             2069..2400
                     /name="qacG"
                     /gene="qacG"
                     /gene_name="small multidrug resistance (SMR) antibiotic
                     efflux pump"
                     /tool="rgi"
                     /drug_class="disinfecting agents and antiseptics"
                     /identity="41.35"
                     /coverage="102.8"
     Typing          complement(2668..3148)
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="84.867"
                     /coverage="93.0"
                     /evalue="1.73e-132"
     Typing          509..1998
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="91.784"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 ttaccactcc gtcataacat gtttagccag tatacactcc gctagcgcta cgtgactggt
       61 tcagggctgc gccccgaaac ccgctaaaac cactgccgcg tctgcggctt gtccaacacc
      121 gtgccgaccg ggaaaagatt tcccgtccgt cccggcgtta tcaggaggcc ggattggcag
      181 acaaacgcag caaaatgctc accatgtggg tgactgagga cgagcaccgg cgtctgctgg
      241 agcgctgcga gggtaagcaa ctcgcggcct ggatgcggca gacgtgcctg gacgagaagc
      301 ccgcacgcgc cggcaaactt ccgtcgatct cgccggcgct gcttcgtcag cttgccggca
      361 tggggaacaa ccttaaccag attgcccgcc aggttaacgc cggtggtgga agcggacacg
      421 accgcgtgca gattgtcgcc gcgctgatgg ccatcgatgc cggactcgag cggttgcggc
      481 atgccgtact ggaaaagggt gctgatgatg atcgttaagt ttcatcctcg ggggcgcggc
      541 ggcggcggcg ggccggtcga ttatctgctg ggtaaagatc gccagcgcga cggcgccagc
      601 gttctgcagg ggaagccgga tgaagtccgg gagcttatcg acgcctcgcc ctacgccaaa
      661 aagtacactt ccggcgttct gtcttttgcc gaacaggatt taccgcccgg ccagcgcgaa
      721 aagctgatgg cgagtttcga gcgggttctg atgcccggac tcgataaaga ccagtacagc
      781 gtgctgtggg ttgagcaccg ggacaagggg cggctggagc tgaacttcct gatcccgaac
      841 acggagcttc tgaccggcaa gcggctacag ccgtattacg accgcgccga ccgtccgcgc
      901 atcgatgcct ggcagaccat agtgaacggc aggctggggc tgcatgaccc gaacgcgccg
      961 gagaaccggc gggtgctggt ctcgccttcc gcgctgccgg aagcgaagca ggaagccgcc
     1021 caggcgatta cgagcggcct gcttgccctt gcctcatccg gggagctgaa aacgcgtcag
     1081 gacgtcactg aggcgctgga aagcgcaggt tttgaggtgg tgcgcaccac aaaaagcagc
     1141 atcagcattg ccgacccgga cggggggcga aacatccgac tgaagggagc catctatgaa
     1201 cagtctttta acgctggcga aggacttaga gcagaaatcg aaagcgcagc agcagactac
     1261 cggcgagatg ctgaaagccg cattcagcga gcacgagaag tctgtcagag cggaactgag
     1321 cgaaagcgag aagagaatca gcgccgccat cctcgaccac gaccggaagc tgtcctcagc
     1381 catgagccag cgcacgaaag ggatgctgcg catggtcagc cagacgtggc tgaccatcgt
     1441 tctggtctcc gcgctgctga tagcgtcgag cgcgggcatt ctgtggtggc aggggcagca
     1501 gatactcgag aactatacga ccatccggga gcagaagagc acgcaggcca tgctgtcaga
     1561 gaggaacagc ggcgtacagc tctcgacctg cggcgagcag agacgccgct gcgtgagggt
     1621 gaacccggaa gcgggacagt tcggagagga ctcgagctgg atgatactgg cggggaaata
     1681 gcacatgacg gagctggaaa aacagttgct gagcgcatta gagcagctac agcaggacta
     1741 ctcgaaaagg ctggacgagt gggagaacgc cttcgcggaa tggcggacga tgtctggtct
     1801 tattcaacgg gagaacgcgg cgctgaacga gcgcgtcacg gtcttgagca ggcaggtgca
     1861 gagtttgagc gagcagctgc gccggttgtc gaaaggctga acgcgataga aactcaccgg
     1921 cagcaggagc gggcggtgca gcaccagaaa gcgctggagc tggaacgatc gcagtggcag
     1981 catcatggtc cttcgctatg ataagccggg ttctattaat tagttgttat gtaaagatat
     2041 ttaaattttt caacatgagg tatttttatg aatacatata ttttcctggg aggggctatt
     2101 gtggctgaag tcataggcac cacctttatg aaatattcag aaggatttac acgcctgtgg
     2161 ccgtctgtag gaactgcaat aagttattgc gctgcctttt atctgctttc ccaaacattg
     2221 gaaaatatac ctaccggtat tgcttacgca atttggtctg gtgcgggaat tgtactaata
     2281 agcctagctg gttggttggt tagcggtcaa aaacttgatt ggcccgcaat tgcaggaatg
     2341 ttccttattt gcgttggagt tttggttatt aatatttttt caaaatcttc tgcgcattaa
     2401 ctttttaccg aagcatattg gccaaagcaa aaggatttcc ttgagatcct ttctttctgc
     2461 gcgtaatctt tttccctgta aacaaaaaaa ccaccgctac caacggtggt tcgtttgccg
     2521 gaattcagag ctaccaactc tttgttccga tggtaactgg ctttcacgga gcgcagatac
     2581 ctaaactgtc ctttcagtgt agccgcactc aggctttcac ttcaagaact ctgtaggcat
     2641 tctctacaat ctctgctccg tgaacccagt taccaatggc taccgccact ggcgatttgt
     2701 cgtgtctttc cgggttggac tcaagttgat agttaccgga acgggcgtgg tagtcggact
     2761 gaacgggggg ttcgtgcata cagtccatcc tggagcgaac tgcctacccg gaaccgagtt
     2821 tcaggcgtgg aatgagaaac cgcggccata acagcggaat gacaccggta aaccgaaagg
     2881 caggaatgcg ggggagcacg agggagccac cagggggaaa cgcctggtat ctttaagccg
     2941 catcgggttt cgccaccgct gatttgagcg tcattttcgt gatactcgtc aggggggcgg
     3001 agcctatgga aaaacggcaa ctccgcatcc ttttcttggc ctttcctgct tcgagcggca
     3061 tttgcttatc ccgtaatctg tgagctggta acgcccgccg cagtctcacg gcaggagcgt
     3121 agcgaccgag tgagcgagga ggcgtcattt catcaccacc tgcattttgc acttacgccc
     3181 ggcagccgca tttttcccct gtcacatgaa gcactttcat aaa
//
