LOCUS       NZ_CP062991             4897 bp    DNA     linear   CON 16-MAY-2024
DEFINITION  Klebsiella pneumoniae strain CriePir26 plasmid unnamed5, complete
            sequence.
ACCESSION   NZ_CP062991
VERSION     NZ_CP062991.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN13153735
            Assembly: GCF_009661485.2
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 4897)
  AUTHORS   Shelenkov,A., Mikhaylova,Y., Yanushevich,Y., Samoilov,A.,
            Petrova,L., Fomina,V., Gusarov,V., Zamyatin,M., Shagin,D. and
            Akimkin,V.
  TITLE     Molecular Typing, Characterization of Antimicrobial Resistance,
            Virulence Profiling and Analysis of Whole-Genome Sequence of
            Clinical Klebsiella pneumoniae Isolates
  JOURNAL   Antibiotics (Basel) 9 (5), 261 (2020)
   PUBMED   32429555
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4897)
  AUTHORS   Shelenkov,A., Mikhaylova,Y., Yanushevich,Y., Petrova,L., Fomina,V.,
            Zamyatin,M. and Shagin,D.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-SEP-2020) Department Of Molecular Diagnostics and
            Epidemiology, Central Research Institute of Epidemiology,
            Novogireevskaya str., 3a, Moscow 111123, Russia
REFERENCE   3  (bases 1 to 4897)
  AUTHORS   Shelenkov,A., Mikhaylova,Y., Yanushevich,Y., Samoilov,A.,
            Petrova,L., Fomina,V., Gusarov,V., Zamyatin,M. and Shagin,D.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-OCT-2019) Department Of Molecular Diagnostics and
            Epidemiology, Central Research Institute of Epidemiology,
            Novogireevskaya str., 3a, Moscow 111123, Russia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.8-beta
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 87x
            Sequencing Technology             :: Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_009661485.2-RS_2024_05_16
            Annotation Date                   :: 05/16/2024 03:55:03
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,501
            CDSs (total)                      :: 5,376
            Genes (coding)                    :: 5,261
            CDSs (with protein)               :: 5,261
            Genes (RNA)                       :: 125
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 52 of 115
            Pseudo Genes (incomplete)         :: 63 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 18 of 115
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP062991.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4897
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="CriePir26"
                     /isolation_source="traumatic discharge"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="unnamed5"
                     /geo_loc_name="Russia: Moscow"
                     /lat_lon="55.76 N 37.62 E"
                     /collection_date="2017"
                     /collected_by="Pir Nat Surg Centre"
     gene            complement(<1..549)
                     /locus_tag="GJJ26_RS27335"
                     /old_locus_tag="GJJ26_027335"
     CDS             complement(<1..549)
                     /locus_tag="GJJ26_RS27335"
                     /old_locus_tag="GJJ26_027335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000635827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="WP_193819349.1"
                     /translation="MIVKIHSRGVGRGSGPVEYLLGKDGNRSGATLDRGDPEMVRELID
                     GSRYAQRYTSGVLSFHEPDLPRETKDRLMDEFEQALLPGLDADQYAVLWVEHRDKDRLE
                     LNFVVPNVELLSGKRLQPYFHGADGHRINAWRTAINGDLGLHDPDDPINRQAVTTAKDL
                     PRNKLEAEQAITGGLLGMAA"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(530..868)
                     /locus_tag="GJJ26_RS27340"
                     /old_locus_tag="GJJ26_027340"
     CDS             complement(530..868)
                     /locus_tag="GJJ26_RS27340"
                     /old_locus_tag="GJJ26_027340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019820367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_094965905.1"
                     /translation="MPTEQPKRLKTIKVRVTETEWASLRAACPKAQLAEWMREYCLGVV
                     PQQRRTPPPTIDPALLRQVAGIGNNLNQIARRINASPLTTLDKVEYLAALAAVARELEG
                     LRRDRQNP"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            1067..1357
                     /locus_tag="GJJ26_RS27345"
                     /old_locus_tag="GJJ26_027345"
     CDS             1067..1357
                     /locus_tag="GJJ26_RS27345"
                     /old_locus_tag="GJJ26_027345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005321770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_094965911.1"
                     /translation="MKDNADKGTGDMFGIKRGRGRPKTGSAKTGAERQAAYRTKQQENN
                     VTVTINRGLLEGLDAQMQAIRDGGSAVVLTSEQAGEILRALRTAEKKQLRG"
                     /name="hypothetical protein"
     gene            complement(1510..2496)
                     /locus_tag="GJJ26_RS27350"
                     /old_locus_tag="GJJ26_027350"
     CDS             complement(1510..2496)
                     /locus_tag="GJJ26_RS27350"
                     /old_locus_tag="GJJ26_027350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005321767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_094965910.1"
                     /translation="MTEKAFLRNLPHRPYCTDDKSSGLLIRPQATALGYRHIQYNPPPH
                     VASLVFDLDKPDSYHAWQDAGLPAPHWISVNRTNGHAHIGYMLATPVARTSAARQKPLR
                     YLAAIEHVLAKRLGADMGYAGLITKNPTHSDWLTIWHQIEPYSLDYLAEFCPDADLAAY
                     SRRSRKEATGLGRNVTVFDNVREWAYTAIRAHWRPNGYDAWLCAVQAACECTNVFGLEQ
                     GGPLPHSEIKATAKSIARWTWRNLTPSTFADYVDRTHTPEIQAVRGAKGGRIGGKVSKG
                     GGRPSKAASLLPVVLQLKAQGYSNRDIAEDLQISAGSVSNYLRRDRE"
                     /name="replication initiation protein"
     gene            complement(2658..3089)
                     /locus_tag="GJJ26_RS27355"
                     /old_locus_tag="GJJ26_027355"
     CDS             complement(2658..3089)
                     /locus_tag="GJJ26_RS27355"
                     /old_locus_tag="GJJ26_027355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019535530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6530 family protein"
                     /protein_id="WP_094965909.1"
                     /translation="MNIPTHLKHKPVLEVINYDQIDGPYANDSDAMGLSIGIAQWNSPG
                     WTDLSAKVWRHTGEKWSRQSEELPLHRVIDLTTLICIAMEYCRNGRLSSSDDFPVSRST
                     DNADLGHHVELMKKELKSGSKYLDESLKRLSLELKRLGY"
                     /name="DUF6530 family protein"
     gene            complement(3204..3512)
                     /locus_tag="GJJ26_RS27360"
                     /old_locus_tag="GJJ26_027360"
     CDS             complement(3204..3512)
                     /locus_tag="GJJ26_RS27360"
                     /old_locus_tag="GJJ26_027360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014839830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CcdB family protein"
                     /protein_id="WP_094965908.1"
                     /translation="MQFTIYRNKGNARAYPYLLDVQSDIIGELATRIVIPLFPLAAFTG
                     RPAQRLNPVIIVEGDKYLVMTQDMAAVRQTQLGEEVTGVQESRQLIKDAIDFLMDGV"
                     /name="CcdB family protein"
     gene            complement(3512..3751)
                     /locus_tag="GJJ26_RS27365"
                     /old_locus_tag="GJJ26_027365"
     CDS             complement(3512..3751)
                     /locus_tag="GJJ26_RS27365"
                     /old_locus_tag="GJJ26_027365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001118105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system CcdA family
                     antitoxin"
                     /protein_id="WP_094965907.1"
                     /translation="MVAAAMRSKRTVSVTLEPSLLERARAAGINLSATLAAALQHEISG
                     SEAERWKRENSAAIQELNRITEEHGLLSDDYRAF"
                     /name="type II toxin-antitoxin system CcdA family
                     antitoxin"
     gene            complement(3875..4090)
                     /locus_tag="GJJ26_RS27370"
                     /old_locus_tag="GJJ26_027370"
     CDS             complement(3875..4090)
                     /locus_tag="GJJ26_RS27370"
                     /old_locus_tag="GJJ26_027370"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_153913310.1"
                     /translation="MIAQQTAQTQEGLLDEYQQAHAERDLYFNRKHEQKEISGLPIDKA
                     REYLQEGLEGRIERQQVRERGRGFEM"
                     /name="hypothetical protein"
     gene            complement(4087..4320)
                     /locus_tag="GJJ26_RS27375"
                     /old_locus_tag="GJJ26_027375"
     CDS             complement(4087..4320)
                     /locus_tag="GJJ26_RS27375"
                     /old_locus_tag="GJJ26_027375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD/MobD family mobilization/exclusion protein"
                     /protein_id="WP_153913308.1"
                     /translation="MTELERHLLNALETLQSEQDRQLSGLQTACESLLRQFETTKKENA
                     SLLGEVKVLRQQCEHLNEQVSSLATQVASLKA"
                     /name="MbeD/MobD family mobilization/exclusion protein"
     gene            complement(4324..4758)
                     /locus_tag="GJJ26_RS27380"
                     /old_locus_tag="GJJ26_027380"
     CDS             complement(4324..4758)
                     /locus_tag="GJJ26_RS27380"
                     /old_locus_tag="GJJ26_027380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011069631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_153913306.1"
                     /translation="MSKILDMAKTFEQTSKQQADDIESSLKPEFERHERAIKEALDSSR
                     QSINDAIQDQQKLIGWLLLKSWGVMLISGLLLLAATSGTLWYQGNLIAERQATLEALGK
                     KGGKLIFNKCEDENGKIRLCIRMNMDEGKFGDDYMIPKGY"
                     /name="MbeB family mobilization protein"
     Typing          complement(3879..4897)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="89.443"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 cgcagccatg cccagcaagc cgcctgtaat ggcttgttcg gcttccagct tgttccgggg
       61 taggtctttg gccgtagtca cggcctgccg attgattggg tcgtctgggt cgtgcaatcc
      121 caagtcgcca ttgatggccg ttcgccatgc gttgatccgg tggccgtccg ccccgtggaa
      181 atacggctga aggcgtttgc cggacaacag ttcgacgttc ggcaccacga aattcagctc
      241 taggcggtct ttgtctcggt gctcgaccca cagcaccgca tactgatcgg cgtccagtcc
      301 gggcaacagg gcttgctcga actcgtccat caaccgatcc ttggtctccc tcggcaagtc
      361 cggttcatgg aacgacagca cgccgctggt gtaccgctgg gcgtaacggc tgccgtctat
      421 caactccctg accatctcgg ggtcgccgcg atccagcgtg gccccgctcc ggttcccgtc
      481 tttgccaagc aggtattcca ccgggccaga tccccgaccg actccccggc tatggatttt
      541 gacgatcacg gcgtagcccc tccagttctc gggccacggc ggctaacgcc gcaaggtatt
      601 ccaccttgtc caacgtggtc agcgggctgg cattgatgcg ccgggcgatc tggttgaggt
      661 tgttcccgat cccggccacc tgccggagca aggccggatc gatggttggc ggtggggtgc
      721 gccgctgctg gggaacaacc cccaagcagt actcgcgcat ccactccgcc aactgggctt
      781 tggggcaggc cgcccgcaag ctggcccact cggtttcagt gactctgacc ttgatcgttt
      841 tgagccgttt tggctgctct gtcggcatcg atggcctccg tttttttcgg gggtttcggg
      901 gggcttgccc cctgaacaag gccacagggg atagaccgcg aagcgggccc cctgtgtgtc
      961 cttgcttagt actttaacat gggttggatt ttgagggtgc ggattaatgg tttttgtgtc
     1021 acagtgacag ataatattta tggtcactgt gacgggaatt aggacgatga aagacaacgc
     1081 tgataaggga accggagaca tgtttggcat caagcgcgga cggggtagac ccaaaaccgg
     1141 atcagccaaa acaggggcag aacgacaagc ggcctaccgg acaaaacagc aggagaataa
     1201 cgttactgtg acgataaata gagggctatt ggaaggactg gatgcccaga tgcaggcgat
     1261 cagagacggg ggttctgcgg tggtgctgac ctcggaacag gccggggaga tcttgcgggc
     1321 gctacgaaca gcagagaaaa aacagctccg gggataacag ctactgccca caggccgaac
     1381 acatggcctg atggacagcc ctacgggatc gccgttaccc cccctcgcca agatcctctc
     1441 tcttgaatgc ttttagggct caaggaacgc cctacgggcg ctgttatctg atataggctt
     1501 tgtttttgat cactcacgat cacggcggag gtagttggac acactgccag ccgaaatttg
     1561 cagatcctcc gcaatatcgc gattgctgta accctgcgcc ttgagttgta gcaccacagg
     1621 taggagagaa gcagctttgc tcggcctgcc tccgcccttg gataccttgc caccgatacg
     1681 cccgccctta gcccctctga cggcctgaat ctctggtgta tgcgtccgat caacataatc
     1741 cgcgaacgtg gacggggtta gattccgcca cgtccaacgg gcgatgctct tggccgtggc
     1801 cttgatctcg ctatggggca acgggccacc ctgctccaag ccaaacacgt tggtgcactc
     1861 acaggcagct tgcacggcac agagccatgc atcgtaccca ttaggccgcc aatgcgctct
     1921 aatcgccgtg taggcccatt cccgcacgtt gtcgaacaca gtgacgttac ggcctagccc
     1981 agtcgcctct ttgcgactgc ggcggctgta ggccgccaaa tcggcatccg ggcagaactc
     2041 tgcgagatag tcgaggctgt agggctcgat ttggtgccag atggtgagcc aatcgctatg
     2101 ggtggggttt ttggtgatca gccccgcata ccccatgtcg gcccccagcc gcttcgctag
     2161 aacgtgttct atggcggcta ggtatcgcag cggcttttgc cgggcggcgc tggtacgggc
     2221 tactggggtc gccagcatgt acccaatatg ggcgtggccg ttcgtgcggt taacgctgat
     2281 ccagtgcggg gctggcaagc ctgcatcctg ccaagcgtgg taactgtccg gtttatccaa
     2341 gtcgaacacc aaagacgcca catgcggcgg tgggttgtac tggatatggc gatagcccag
     2401 tgcggtagcc tgcgggcgaa tgagcagccc tgacgattta tcgtcagtgc aatagggccg
     2461 atgggggaga ttcctgagaa aggctttttc tgtcatcgat caccgcgctt attggcggtg
     2521 cagggttgtt tggtgtgatc catgtggtag acttctcttg tgattcgtca cagcccccgt
     2581 ttttcgcaga tccgccaaga ttccagcgat aacgggggtt tgtcttttta tgagcaataa
     2641 gttaccgcat agccccgtta gtaacccagc ctttttagct ctaaagacag tcgttttaga
     2701 ctttcatcga gatatttact cccgctcttg agctcttttt tcattagctc aacatgatgc
     2761 cccaaatcag cgttatcagt agacctgcta accgggaaat cgtcgctaga tgacaagcga
     2821 ccattgcgac agtattccat cgcgatacat atcaatgtcg ttaagtcgat aacacgatgc
     2881 agtggtagtt cttctgactg tcttgaccat ttctcgcctg tatgacgcca tactttggct
     2941 gagaggtcag tccatccagg tgagttccat tgtgcgatgc caattgacag tcccattgca
     3001 tcagagtcat tagcatatgg accatcaatc tggtcatagt taataacttc cagtaccggc
     3061 ttgtgcttca aatgagttgg aatgttcata gcgagcccca gattatcaag tttactaatt
     3121 tcacaaacat tactgagttt agttggtaat gtctatgggg ttgtacgccg gaaccgccga
     3181 aatatctgcc tccctttaaa gcctcaaaca ccatccatca ggaaatctat tgcgtccttg
     3241 atgagctgac gagactcctg cactcctgtg acttcttccc caagctgcgt ctgcctcacc
     3301 gctgccatgt cctgcgtcat caccagatac ttatccccct caacgataat taccggattc
     3361 agccgttgcg ctggccgccc ggtgaaggcc gccagcggga acagtgggat cactattctg
     3421 gtggccagct cgccgatgat gtcgctttgc acatccagca gataggggta agcgcgggca
     3481 ttgcccttgt tgcggtagat cgtgaactgc atcagaacgc cctgtaatcg tcggaaagca
     3541 aaccgtgctc ctcggtgatg cggttaagct cctgtatggc tgcactattc tcacgcttcc
     3601 agcgttctgc ttctgatccg ctgatttcat gctgtagtgc ggctgcaagg gtggcggaca
     3661 ggttgatccc cgccgctctg gctcgttcca gcaagctggg ctccagcgtg acgctgaccg
     3721 ttctcttgct gcgcattgct gctgcgacca tgatgacctc caataaatta acacgtgcga
     3781 tagcacgtgt atgataatat cacgcccttg cgacgggtgc gactagcgaa gtctgtcgcg
     3841 ctcggggcag gggccgccaa gcgcagcgcg gcagctacat ctcaaagcca cgcccccgtt
     3901 cgcggacctg ctgccgctct atccgcccct ccagtccttc ctgtaggtac tctctggcct
     3961 tgtctatcgg caagccggag atctctttct gctcgtgctt ccgattgaaa tagagatccc
     4021 gttctgcgtg ggcctgctgg tattcgtcca ataacccctc ctgcgtctgc gccgtctgct
     4081 gcgcgatcat gccttcaagc tggcgacctg cgttgccaag ctgctgacct gctcgttcaa
     4141 gtgctcgcac tgctggcgca agactttcac ttctcccagc aagctcgcgt tttctttttt
     4201 cgtcgtctcg aactgccgta gcaagctctc gcacgctgtt tgcaagccgc tcaattgccg
     4261 atcttgctcg ctctgcaagg tctctagcgc gttcaataag tgccgttcta gctcggtcat
     4321 cgtttagtag cccttcggta tcatgtaatc gtccccgaac ttcccttcgt ccatgttcat
     4381 cctgatgcaa agccggatct tcccgttctc gtcctcgcac ttgttgaaga tcaatttccc
     4441 gccctttttg cccaacgcct ccaaggtggc ctgccgctcg gcgatcaggt tcccctgata
     4501 ccacagcgtc ccgctcgtcg cggccagcaa cagcaagccc gagatcaaca tcactcccca
     4561 gctcttgagc aggagccagc cgatcagctt ctgctggtct tggatggcgt cgttgatgct
     4621 ctgcctgctt gagtccaacg cttctttgat agctcgctcg tgcctctcga actctggctt
     4681 cagactgctc tctatatcgt cggcttgctg cttcgatgtc tgctcgaagg tctttgccat
     4741 gtctaaaatc ttgctcatag atcgccccct ttaggcggat attccggccc ccttcagggt
     4801 cggcaatgct gatactgttc ttggttgtcc tggtaacgtc gaaccccgcc ccggtcaggg
     4861 tcttgagcac gtcctcccgg tttttaacca gccccgc
//
