LOCUS       NZ_CP062990             8350 bp    DNA     circular CON 16-MAY-2024
DEFINITION  Klebsiella pneumoniae strain CriePir26 plasmid unnamed4, complete
            sequence.
ACCESSION   NZ_CP062990
VERSION     NZ_CP062990.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN13153735
            Assembly: GCF_009661485.2
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 8350)
  AUTHORS   Shelenkov,A., Mikhaylova,Y., Yanushevich,Y., Samoilov,A.,
            Petrova,L., Fomina,V., Gusarov,V., Zamyatin,M., Shagin,D. and
            Akimkin,V.
  TITLE     Molecular Typing, Characterization of Antimicrobial Resistance,
            Virulence Profiling and Analysis of Whole-Genome Sequence of
            Clinical Klebsiella pneumoniae Isolates
  JOURNAL   Antibiotics (Basel) 9 (5), 261 (2020)
   PUBMED   32429555
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8350)
  AUTHORS   Shelenkov,A., Mikhaylova,Y., Yanushevich,Y., Petrova,L., Fomina,V.,
            Zamyatin,M. and Shagin,D.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-SEP-2020) Department Of Molecular Diagnostics and
            Epidemiology, Central Research Institute of Epidemiology,
            Novogireevskaya str., 3a, Moscow 111123, Russia
REFERENCE   3  (bases 1 to 8350)
  AUTHORS   Shelenkov,A., Mikhaylova,Y., Yanushevich,Y., Samoilov,A.,
            Petrova,L., Fomina,V., Gusarov,V., Zamyatin,M. and Shagin,D.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-OCT-2019) Department Of Molecular Diagnostics and
            Epidemiology, Central Research Institute of Epidemiology,
            Novogireevskaya str., 3a, Moscow 111123, Russia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.8-beta
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 87x
            Sequencing Technology             :: Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_009661485.2-RS_2024_05_16
            Annotation Date                   :: 05/16/2024 03:55:03
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,501
            CDSs (total)                      :: 5,376
            Genes (coding)                    :: 5,261
            CDSs (with protein)               :: 5,261
            Genes (RNA)                       :: 125
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 52 of 115
            Pseudo Genes (incomplete)         :: 63 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 18 of 115
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP062990.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..8350
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="CriePir26"
                     /isolation_source="traumatic discharge"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="unnamed4"
                     /geo_loc_name="Russia: Moscow"
                     /lat_lon="55.76 N 37.62 E"
                     /collection_date="2017"
                     /collected_by="Pir Nat Surg Centre"
     gene            1..840
                     /locus_tag="GJJ26_RS27285"
                     /old_locus_tag="GJJ26_027285"
     CDS             1..840
                     /locus_tag="GJJ26_RS27285"
                     /old_locus_tag="GJJ26_027285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001517559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase RepA family protein"
                     /protein_id="WP_000190708.1"
                     /translation="MATHKPINILEAFAAAPPPLDYVLPNMVAGTVGALVSPGGAGKSM
                     LALQLAAQIAGGPDLLEVGELPTGPVIYLPAEDPPTAIHHRLHALGAHLSAEERQAVAD
                     GLLIQPLIGSLPNIMAPEWFDGLKRAAEGRRLMVLDTLRRFHIEEENASGPMAQVIGRM
                     EAIAADTGCSIVFLHHASKGAAMMGAGDQQQASRGSSVLVDNIRWQSYLSSMTSAEAEE
                     WGVDDDQRRFFVRFGVSKANYGAPFADRWFRRHDGGVLKPAVLERQRKSKGVPRGEA"
                     /name="helicase RepA family protein"
     gene            827..1678
                     /gene="repC"
                     /locus_tag="GJJ26_RS27290"
                     /old_locus_tag="GJJ26_027290"
     CDS             827..1678
                     /gene="repC"
                     /locus_tag="GJJ26_RS27290"
                     /old_locus_tag="GJJ26_027290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000240539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein C, IncQ-type"
                     /protein_id="WP_000240536.1"
                     /translation="MVKPKNKHSLSHVRHDPAHCLAPGLFRALKRGERKRSKLDVTYDY
                     GDGKRIEFSGPEPLGADDLRILQGLVAMAGPNGLVLGPEPKTEGGRQLRLFLEPKWEAV
                     TADAMVVKGSYRALAKEIGAEVDSGGALKHIQDCIERLWKVSIIAQNGRKRQGFRLLSE
                     YASDEADGRLYVALNPLIAQAVMGGGQHVRISMDEVRALDSETARLLHQRLCGWIDPGK
                     TGKASIDTLCGYVWPSEASGSTMRKRRQRVREALPELVALGWTVTEFAAGKYDITRPKA
                     AG"
                     /name="repC"
     gene            <1853..2500
                     /locus_tag="GJJ26_RS27295"
                     /old_locus_tag="GJJ26_027295"
                     /pseudo=""
     CDS             <1853..2500
                     /locus_tag="GJJ26_RS27295"
                     /old_locus_tag="GJJ26_027295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000480972.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase family protein"
                     /name="aminoglycoside phosphotransferase family protein"
     gene            2905..3684
                     /locus_tag="GJJ26_RS27300"
                     /old_locus_tag="GJJ26_027300"
     CDS             2905..3684
                     /locus_tag="GJJ26_RS27300"
                     /old_locus_tag="GJJ26_027300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000422636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APH(3')-VI family aminoglycoside
                     O-phosphotransferase"
                     /protein_id="WP_094965899.1"
                     /translation="MKLPNIIQQFIGNSVLEPNKIGQSPSDVYSFNRNNETFFLKRSST
                     LYTATTYSVSREAKMLSWLSEKLKVPELIMTFQDEQFEFMITKAINAKPISALFLTDQE
                     LLAIYKEVLNLLNSIAIIDCPFISNIDHRLKESKFFIDNQLLDDIDQDDFDAELWGDHK
                     TYLSLWNELNETRVEERLVFSHGDITDSNIFIDKFNEIYFLDLGRAGLADEFVDISFVE
                     RCLREDASEETAKIFLKHLKNDRPDKRNYFLKLDELN"
                     /name="APH(3')-VI family aminoglycoside
                     O-phosphotransferase"
     gene            3728..3928
                     /locus_tag="GJJ26_RS27305"
                     /old_locus_tag="GJJ26_027305"
     CDS             3728..3928
                     /locus_tag="GJJ26_RS27305"
                     /old_locus_tag="GJJ26_027305"
                     /inference="COORDINATES: protein motif:HMM:NF016876.4"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]; GO:0009432 - SOS response [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_094965900.1"
                     /translation="MLWTVDAEQDRERIYDYLDERNPIAAIELDDLIREKVSLLAHNNL
                     MGRTGQKKGTRELKTMSEANP"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(<4269..4673)
                     /locus_tag="GJJ26_RS27310"
                     /old_locus_tag="GJJ26_027310"
                     /pseudo=""
     CDS             complement(<4269..4673)
                     /locus_tag="GJJ26_RS27310"
                     /old_locus_tag="GJJ26_027310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001120888.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="transposase zinc-binding domain-containing
                     protein"
                     /name="transposase zinc-binding domain-containing protein"
     gene            complement(5227..5511)
                     /locus_tag="GJJ26_RS27315"
                     /old_locus_tag="GJJ26_027315"
     CDS             complement(5227..5511)
                     /locus_tag="GJJ26_RS27315"
                     /old_locus_tag="GJJ26_027315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000238497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein C"
                     /protein_id="WP_000238497.1"
                     /translation="MVKGSNKAADRLAKLEEQRARINAEIQRVRAREQQQERKNETRRK
                     VLVGAMILAKVNSSEWPEDRLMAAMDAYLERDHDRALFGLPPRQKDEPG"
                     /name="mobilization protein C"
     gene            5711..>6352
                     /locus_tag="GJJ26_RS27595"
                     /pseudo=""
     CDS             5711..>6352
                     /locus_tag="GJJ26_RS27595"
                     /inference="COORDINATES: protein motif:HMM:NF015354.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /name="MobA/MobL family protein"
     gene            6459..6872
                     /locus_tag="GJJ26_RS27600"
     CDS             6459..6872
                     /locus_tag="GJJ26_RS27600"
                     /inference="COORDINATES: protein motif:HMM:NF036929.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncQ-type mobilization protein MobB"
                     /protein_id="WP_010890157.1"
                     /translation="MNAIDRVKKSRGINELAEQIEPLAQSMATLADEARQVMSQTQQAS
                     EAQAAEWLKAQRQTGAAWVELAKELREVAAEVSSAAQSARSASRGWHWKLWLTVMLASM
                     MPTVVLLIASLLLLDLTPLTTEDGSIWLRLVAR"
                     /name="IncQ-type mobilization protein MobB"
     gene            6971..7840
                     /locus_tag="GJJ26_RS27605"
     CDS             6971..7840
                     /locus_tag="GJJ26_RS27605"
                     /inference="COORDINATES: protein motif:HMM:NF028103.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-primase RepB domain-containing protein"
                     /protein_id="WP_229382459.1"
                     /translation="MNREWSAAEVLQNTPWLKRMNAQGNDVYIRPAEQERHGLVLVDDL
                     SEFDLDDMKAEGREPALVVETSPKNYQAWVKVADAAGGELRGQIARTLASEYDADPASA
                     DSRHYGRLAGFTNRKDKHTTRAGYQPWVLLRESKGKTATAGPALVQQAGQQIEQAQRQQ
                     EKARRLASLELPERQLSRHRRTALDEYRSEMAGLVKRFGDDLSKCDFIAAQKLASRGRS
                     AEEIGKAMAEASPALAERKPGHEADYIERTVSKVMGLPSVQLARAELARAPAPRQRGMD
                     RGGPDFSM"
                     /name="DNA-primase RepB domain-containing protein"
     gene            7901..8113
                     /locus_tag="GJJ26_RS27325"
                     /old_locus_tag="GJJ26_027325"
     CDS             7901..8113
                     /locus_tag="GJJ26_RS27325"
                     /old_locus_tag="GJJ26_027325"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000455501.1"
                     /translation="MEYEKSASGSVYLIKSDKGYWLPGGFGYTSNKAEAGRFSVADMAS
                     LNLDGCTLSLFREDKPFGPGKFLGD"
                     /name="hypothetical protein"
     gene            8115..8321
                     /gene="urfF"
                     /locus_tag="GJJ26_RS27330"
                     /old_locus_tag="GJJ26_027330"
     CDS             8115..8321
                     /gene="urfF"
                     /locus_tag="GJJ26_RS27330"
                     /old_locus_tag="GJJ26_027330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007857788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid repression protein F"
                     /protein_id="WP_007857788.1"
                     /translation="MKDQKDKQTGDLLASPDAVRQARYAERMKAKGMRQRKFWLTDDEY
                     EALRECLEELRAAQGGGSDPASA"
                     /name="urfF"
     ARG             2906..3681
                     /name="aph(3')-VIa"
                     /gene="aph(3')-VIa"
                     /gene_name="aminoglycoside O-phosphotransferase
                     APH(3')-VIa"
                     /tool="amrfinderplus"
                     /drug_class="AMINOGLYCOSIDE"
                     /identity="98.07"
                     /coverage="100.0"
     ARG             1872..2500
                     /name="APH(6)-Id"
                     /gene="APH(6)-Id"
                     /gene_name="APH(6)"
                     /tool="rgi"
                     /drug_class="aminoglycoside antibiotic"
                     /identity="99.04"
                     /coverage="75.18"
     Typing          5466..5622
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="6.38e-77"
     Typing          19..840
                     /name="IncQ1"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="96.0"
                     /evalue="0.0"
     Typing          5712..7837
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 atggctaccc ataagcctat caatattctg gaggcgttcg cagcagcgcc gccaccgctg
       61 gactacgttt tgcccaacat ggtggccggt acggtcgggg cgctggtgtc gcccggtggt
      121 gccggtaaat ccatgctggc cctgcaactg gccgcacaga ttgcaggcgg gccggatctg
      181 ctggaggtgg gcgaactgcc caccggcccg gtgatctacc tgcccgccga agacccgccc
      241 accgccattc atcaccgcct gcacgccctt ggggcgcacc tcagcgccga ggaacggcaa
      301 gccgtggctg acggcctgct gatccagccg ctgatcggca gcctgcccaa catcatggcc
      361 ccggagtggt tcgacggcct caagcgcgcc gccgagggcc gccgcctgat ggtgctggac
      421 acgctgcgcc ggttccacat cgaggaagaa aacgccagcg gccccatggc ccaggtcatc
      481 ggtcgcatgg aggccatcgc cgccgatacc gggtgctcta tcgtgttcct gcaccatgcc
      541 agcaagggcg cggccatgat gggcgcaggc gaccagcagc aggccagccg gggcagctcg
      601 gtactggtcg ataacatccg ctggcagtcc tacctgtcga gcatgaccag cgccgaggcc
      661 gaggaatggg gtgtggacga cgaccagcgc cggttcttcg tccgcttcgg tgtgagcaag
      721 gccaactatg gcgcaccgtt cgctgatcgg tggttcaggc ggcatgacgg cggggtgctc
      781 aagcccgccg tgctggagag gcagcgcaag agcaaggggg tgccccgtgg tgaagcctaa
      841 gaacaagcac agcctcagcc acgtccggca cgacccggcg cactgtctgg cccccggcct
      901 gttccgtgcc ctcaagcggg gcgagcgcaa gcgcagcaag ctggacgtga cgtatgacta
      961 cggcgacggc aagcggatcg agttcagcgg cccggagccg ctgggcgctg atgatctgcg
     1021 catcctgcaa gggctggtgg ccatggctgg gcctaatggc ctagtgcttg gcccggaacc
     1081 caagaccgaa ggcggacggc agctccggct gttcctggaa cccaagtggg aggccgtcac
     1141 cgctgatgcc atggtggtca aaggtagcta tcgggcgctg gcaaaggaaa tcggggcaga
     1201 ggtcgatagt ggtggggcgc tcaagcacat acaggactgc atcgagcgcc tttggaaggt
     1261 atccatcatc gcccagaatg gccgcaagcg gcaggggttt cggctgctgt cggagtacgc
     1321 cagcgacgag gcggacgggc gcctgtacgt ggccctgaac cccttgatcg cgcaggccgt
     1381 catgggtggc ggccagcatg tgcgcatcag catggacgag gtgcgggcgc tggacagcga
     1441 aaccgcccgc ctgctgcacc agcggctgtg tggctggatc gaccccggca aaaccggcaa
     1501 ggcttccata gataccttgt gcggctatgt ctggccgtca gaggccagtg gttcgaccat
     1561 gcgcaagcgc cgccagcggg tgcgcgaggc gttgccggag ctggtcgcgc tgggctggac
     1621 ggtaaccgag ttcgcggcgg gcaagtacga catcacccgg cccaaggcgg caggctgacc
     1681 ccccccactc tattgtaaac aagacatttt tatcttttat attcaatggc ttattttcct
     1741 gctaattggt aataccatga aaaataccat gctcagaaaa ggcttaacaa tattttgaaa
     1801 aattgcctac tgagcgctgc cgcacagctc cataggccgc tttcctggct ttgggagggg
     1861 agcagtccgg ttgctcggtc gtgagaacaa tctgatgttg ctcgaatatg ccggggagcg
     1921 aatgctctct cacatcgttg ccgagcacgg cgactaccag gcgaccgaaa ttgcagcgga
     1981 actaatggcg aagctgtatg ccgcatctga ggaacccctg ccttctgccc ttctcccgat
     2041 ccgggatcgc tttgcagctt tgtttcagcg ggcgcgcgat gatcaaaacg caggttgtca
     2101 aactgactac gtccacgcgg cgattatagc cgatcaaatg atgagcaatg cctcggaact
     2161 gcgtgggcta catggcgatc tgcatcatga aaacatcatg ttctccagtc gcggctggct
     2221 ggtgatagat cccgtcggtc tggtcggtga agtgggcttt ggcgccgcca atatgttcta
     2281 cgatccggct gacagagacg acctttgtct cgatcctaga cgcattgcac agatggcgga
     2341 cgcattctct cgtgcgctgg acgtcgatcc gcgtcgcctg ctcgaccagg cgtacgctta
     2401 tgggtgcctt tccgcagctt ggaacgcgga tggagaagag gagcaacgcg atctagctat
     2461 cgcggccgcg atcaagcagg tgcgacagac gtcatactag atatcaagcg acttctccta
     2521 tcccctggga acacatcaat cttaccggag aatatcgttg gccaaagcct tagcgtagga
     2581 tttcgccctc tcccgcaaac gaccccgaca aggcaattga ttaagtgctt aatgattgaa
     2641 tatttgacac aggtcaacgc atcagcttgt ttaagatatg gagaaacaag atgattgatc
     2701 cctaaaatca tagcgcaact cgggtttgat taaaaaaaag cctctttttt aagaggagct
     2761 acagctgtgt cggctgggac aataattttg caagttcttc tagggaataa gccatatttt
     2821 tgctacagtg aaccaaatta agatcatcta tttactaggc ctcgcatttg cggggttttt
     2881 aatactgaat aaaaggaaag cttgatgaaa ttgcccaata ttattcaaca atttattgga
     2941 aacagcgttt tagagccaaa taaaattggt cagtcgccat cggatgttta ttcttttaat
     3001 cgaaataatg aaactttttt tcttaagcga tctagcactt tatatacagc gaccacatac
     3061 agtgtttctc gtgaagcgaa aatgttgagt tggctctctg agaagttaaa ggtgcctgaa
     3121 ctcatcatga cttttcagga tgagcagttt gaattcatga tcactaaagc gatcaatgca
     3181 aaaccaattt cagcgctttt tttaacagac caagaattgc ttgctatcta taaggaggta
     3241 ctcaatctgt taaattcaat tgctattatt gattgtccat ttatttcaaa cattgatcat
     3301 cggttaaaag agtcaaaatt ttttattgat aaccaactcc ttgatgatat agatcaagat
     3361 gattttgacg ctgaattatg gggagaccat aaaacttacc taagtctatg gaatgagtta
     3421 aatgagactc gtgttgaaga aagattggtt ttttctcatg gcgatatcac ggatagtaat
     3481 atttttatag ataaattcaa tgaaatttat tttttagatc ttggccgtgc tgggttagca
     3541 gatgagtttg tagatatatc ctttgttgaa cgttgcctaa gagaggatgc atcggaggaa
     3601 actgctaaaa tatttttaaa gcatttaaaa aatgatagac ctgacaaaag gaattatttt
     3661 ttaaaacttg atgaattgaa ttgattccaa gcattttcta aaaatactta attgtcattt
     3721 tacgtcgatt ctttggaccg tagatgcaga acaagatcgt gaaagaattt atgattatct
     3781 tgatgaaaga aatccaattg ctgcaattga attggatgat ttaattcggg aaaaagttag
     3841 tttgcttgct cataataatc tcatggggag aactggacag aaaaaaggaa caagagagtt
     3901 aaaaactatg agcgaagcga atccatagtc gcttttgctt tttgaagtca caaacaggat
     3961 tagaaaataa ctgcccccac gaccgagcaa agcgaggtca aaaagtacga gcaaagcgag
     4021 tacatagggc agttatccac aggtttttta aattttttct ttttttaata ttttttaata
     4081 tttttaaata tttttagctc ctcgctatgc cttgctctac gtggctttga cgggggtaac
     4141 tatgacagat tgacgcacaa ctatatcaag cgacttctcc tatcccctgg gaacacatca
     4201 atctcaccgg agaatatcgc tggccaaagc cttagcgtag gattccgccc cttcccgcaa
     4261 acgaccccaa acaggaaacg cagctgaaac gggaagctca acacccactg acgcatgggt
     4321 tgttcaggca gtacttcatc aaccagcaag gcggcacttt cggccatccg ccgcgcccca
     4381 cagctcgggc agaaaccgtg acgcttacag ctgaaagcga ccaggtgctc ggcgtggcaa
     4441 gactcgcagc gaacccgtag aaagccatgc tccagccgcc cgcattggag aaattcttca
     4501 aattcccgtt gcacatagcc cggcaattcc tttccctgct ctgccataag cgcagcgaat
     4561 gccgggtaat actcgtcaac gatctgatag agaagggttt gctcgggtcg gtggctctgg
     4621 taacgaccag tatcccgatc ccggctggcc gtcctggccg ccacatgagg catgttccgc
     4681 gtccttgcaa tactgtgttt acatacagtc tatcgcttag cggaaagttc ttttaccctc
     4741 agccgaaatg cctgccgttg ctagacattg ccagccagtg cccgtcactc ccgtactaac
     4801 tgtcacgaac ccctgcaata actgtcacgc ccccctgcaa taactgtcac gaacccctgc
     4861 aataactgtc acgcccccaa acctgcaaac ccagcagggg cgggggctgg cggggtgttg
     4921 gaaaaatcca tccatgatta tctaagaata atccactagg cgcggttatc agcgcccttg
     4981 tggggcgctg ctgcccttgc ccaatatgcc cggccagagg ccggatagct ggtctattcg
     5041 ctgcgctagg ctacacaccg ccccaccgct gcgcggcagg gggaaaggcg ggcaaagccc
     5101 gctaaacccc acaccaaacc ccgcagaaat acgctggagc gcttttagcc gctttagcgg
     5161 cctttccccc tacccgaagg gtgggggcgc gtgtgcagcc ccgcagggcc tgtctcggtc
     5221 gatcattcag cccggctcat ccttctggcg tggcggcaga ccgaacaagg cgcggtcgtg
     5281 gtcgcgttca aggtacgcat ccattgccgc catgagccga tcctccggcc actcgctgct
     5341 gttcaccttg gccaaaatca tggcccccac cagcaccttg cgccttgttt cgttcttgcg
     5401 ctcttgctgc tgttcccttg cccgcacccg ctgaatttcg gcattgattc gcgctcgttg
     5461 ttcttcgagc ttggccagcc gatccgccgc cttgttgctc cccttaacca tcttgacacc
     5521 ccattgttaa tgtgctgtct cgtaggctat catggaggca cagcggcggc aatcccgacc
     5581 ctactttgta gggggagggc gcacttaccg gtttctcttc gagaaactgg cctaacggcc
     5641 acccttcggg cggtgcgctc tccgagggcc attgcatgga gccgaaaagc aaaagcaaca
     5701 gcgaggcagc atggcgattt atcaccttac ggcgaaaacc ggcagcaggt cgggcggcca
     5761 atcggccagg gccaaggccg actacatcca gcgcgaaggc aagtatgccc gcgacatgga
     5821 tgaagtcttg cacgccgaat ccgggcacat gccggagttc gtcgagcggc ccgccgacta
     5881 ctgggatgct gccgacctgt atgaacgcgc caatgggcgg ctgttcaagg aggtcgaatt
     5941 tgccctgccg gtcgagctga ccctcgacca gcagaaggcg ctggcgtccg agttcgccca
     6001 gcacctgacc ggtgccgagc gcctgccgta tacgctggcc atccatgccg gtggcggcga
     6061 gaacccgcac tgccacctga tgatctccga gcggatcaat gacggcatcg agcggcccgc
     6121 cgctcagtgg ttcaagcggt acaacggcaa gaccccggag aagggcgggg cacagaagac
     6181 cgaagcgctc aagcccaagg catggcttga gcagacccgc gaggcatggg ccgaccatgc
     6241 caaccgggca ttagagcggg ctggccacga cgcccgcatt gaccacagaa cacttgaggc
     6301 gcagggcatc gagcgcctgc ccggtgttca cctggggccg aacgtggtgg agatggaagg
     6361 ccggggcatc cgcaccgacc gggcagacgt ggccctgaac atcgacaccg ccaacgccca
     6421 gatcatcgac ttacaggaat accgggaggc aatagaccat gaacgcaatc gacagagtga
     6481 agaaatccag aggcatcaac gagttagcgg agcagatcga accgctggcc cagagcatgg
     6541 cgacactggc cgacgaagcc cggcaggtca tgagccagac ccagcaggcc agcgaggcgc
     6601 aggcggcgga gtggctgaaa gcccagcgcc agacaggggc ggcatgggtg gagctggcca
     6661 aagagttgcg ggaggtagcc gccgaggtga gcagcgccgc gcagagcgcc cggagcgcgt
     6721 cgcgggggtg gcactggaag ctatggctaa ccgtgatgct ggcttccatg atgcctacgg
     6781 tggtgctgct gatcgcatcg ttgctcttgc tcgacctgac gccactgaca accgaggacg
     6841 gctcgatctg gctgcgcttg gtggcccgat gaagaacgac aggactttgc aggccatagg
     6901 ccgacagctc aaggccatgg gctgtgagcg cttcgatatc ggcgtcaggg acgccaccac
     6961 cggccagatg atgaaccggg aatggtcagc cgccgaagtg ctccagaaca cgccatggct
     7021 caagcggatg aatgcccagg gcaatgacgt gtatatcagg cccgccgagc aggagcggca
     7081 tggtctggtg ctggtggacg acctcagcga gtttgacctg gatgacatga aagccgaggg
     7141 ccgggagcct gccctggtag tggaaaccag cccgaagaac tatcaggcat gggtcaaggt
     7201 ggccgacgcc gcaggcggtg aacttcgggg gcagattgcc cggacgctgg ccagcgagta
     7261 cgacgccgac ccggccagcg ccgacagccg ccactatggc cgcttggcgg gcttcaccaa
     7321 ccgcaaggac aagcacacca cccgcgccgg ttatcagccg tgggtgctgc tgcgtgaatc
     7381 caagggcaag accgccaccg ctggcccggc gctggtgcag caggctggcc agcagatcga
     7441 gcaggcccag cggcagcagg agaaggcccg caggctggcc agcctcgaac tgcccgagcg
     7501 gcagcttagc cgccaccggc gcacggcgct ggacgagtac cgcagcgaga tggccgggct
     7561 ggtcaagcgc ttcggtgatg acctcagcaa gtgcgacttt atcgccgcgc agaagctggc
     7621 cagccggggc cgcagtgccg aggaaatcgg caaggccatg gccgaggcca gcccagcgct
     7681 ggcagagcgc aagcccggcc acgaagcgga ttacatcgag cgcaccgtca gcaaggtcat
     7741 gggtctgccc agcgtccagc ttgcgcgggc cgagctggca cgggcaccgg caccccgcca
     7801 gcgaggcatg gacaggggcg ggccagattt cagcatgtag tgcttgcgtt ggtactcacg
     7861 cctgttatac tatgagtact cacgcacaga agggggtttt atggaatacg aaaaaagcgc
     7921 ttcagggtcg gtctacctga tcaaaagtga caagggctat tggttgcccg gtggctttgg
     7981 ttatacgtca aacaaggccg aggctggccg cttttcagtc gctgatatgg ccagccttaa
     8041 ccttgacggc tgcaccttgt ccttgttccg cgaagacaag cctttcggcc ccggcaagtt
     8101 tctcggtgac tgatatgaaa gaccaaaagg acaagcagac cggcgacctg ctggccagcc
     8161 ctgacgctgt acgccaagcg cgatatgccg agcgcatgaa ggccaaaggg atgcgtcagc
     8221 gcaagttctg gctgaccgac gacgaatacg aggcgctgcg cgagtgcctg gaagaactca
     8281 gagcggcgca gggcgggggt agtgaccccg ccagcgccta accaccaact gcctgcaaag
     8341 gaggcaatca
//
