LOCUS       NZ_CP062904             8009 bp    DNA     linear   CON 16-JUN-2023
DEFINITION  Escherichia coli strain Res13-Lact-EB12-05 plasmid unnamed.
ACCESSION   NZ_CP062904
VERSION     NZ_CP062904.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN16304132
            Assembly: GCF_015571955.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8009)
  AUTHORS   Poulin-Laprade,D., Brouard,J.-S., Gagnon,N., Turcotte,A.,
            Langlois,A., Matte,J.J., Carrillo,C.D., Zaheer,R., McAllister,T.,
            Topp,E. and Talbot,G.
  TITLE     Resistance determinants and their genetic context in bacteria from a
            longitudinal study of pigs reared under conventional and
            antibiotic-free husbandry practices
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8009)
  AUTHORS   Poulin-Laprade,D. and Brouard,J.-S.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-OCT-2020) Sciences and Technology, Agriculture and
            Agri-Food Canada, 2000 College, Sherbrooke, Quebec J1M 0C8, Canada
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 13-NOV-2018
            Assembly Method                   :: Unicycler v. 0.4.7
            Assembly Name                     :: QIA-7
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 1.00x
            Sequencing Technology :: Oxford Nanopore MinION and Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 06/16/2023 14:44:38
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,902
            CDSs (total)                      :: 4,782
            Genes (coding)                    :: 4,552
            CDSs (with protein)               :: 4,552
            Genes (RNA)                       :: 120
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 63 of 230
            Pseudo Genes (incomplete)         :: 152 of 230
            Pseudo Genes (internal stop)      :: 47 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            Pseudo Genes (short protein)      :: 4 of 230
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP062904.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..8009
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="Res13-Lact-EB12-05"
                     /serotype="O-untypeable:H11"
                     /isolation_source="feces"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:562"
                     /plasmid="unnamed"
                     /geo_loc_name="Canada: Quebec"
                     /collection_date="2017-03-13"
                     /collected_by="Agriculture and Agri-Food Canada"
     gene            84..671
                     /locus_tag="IMP23_RS00005"
                     /old_locus_tag="IMP23_00005"
     CDS             84..671
                     /locus_tag="IMP23_RS00005"
                     /old_locus_tag="IMP23_00005"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_195714398.1"
                     /translation="MIKKSNVALVENSRIAVKATNFNKQDAIPDGWQKFGNVPPDAVIT
                     MLGTVKEFTYDRKVWRKTPDGTGYMWHPANHQQEQAQNDAGTQNIYCSYVCLGAGYKAF
                     KRAFAAYEGKDKTSIWATLSIMAANARNDNGAWIVKVSQKAIGDELNKDRKAIGRYIKQ
                     LVGAGLIFPIGRYIPGQDGRQYRICYQVEDAK"
                     /name="hypothetical protein"
     gene            668..991
                     /locus_tag="IMP23_RS00010"
                     /old_locus_tag="IMP23_00010"
     CDS             668..991
                     /locus_tag="IMP23_RS00010"
                     /old_locus_tag="IMP23_00010"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_195714399.1"
                     /translation="MSAVISLSEVRRRKETAKEAEIASNWMWVLMLPLSDRYIEVGEDT
                     YWLYVGDNDGSDKSDWIWTRHSELSRDEFLWLINRGVGYFLPPDGCEMEVCYNVEWFSF
                     SRV"
                     /name="hypothetical protein"
     gene            2675..2860
                     /locus_tag="IMP23_RS00015"
                     /old_locus_tag="IMP23_00015"
     CDS             2675..2860
                     /locus_tag="IMP23_RS00015"
                     /old_locus_tag="IMP23_00015"
                     /inference="COORDINATES: protein motif:HMM:NF024139.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_112045314.1"
                     /translation="MKPKLLRVDQVLELLNISRGKLYRLLKNEPLFPRPIKLGGMLYFI
                     PEEIDEFINLQRRVER"
                     /name="helix-turn-helix domain-containing protein"
     gene            2861..3448
                     /locus_tag="IMP23_RS00020"
                     /old_locus_tag="IMP23_00020"
     CDS             2861..3448
                     /locus_tag="IMP23_RS00020"
                     /old_locus_tag="IMP23_00020"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_195714392.1"
                     /translation="MSNLNDIKSIDSVTRITFIEGTINTALLYEAAPVNLTVRVPVNFL
                     ERRHDTKEDGFIRAIMFMAAAGPLKCGGGKNESDLNYVMGVYYGRNIKTDIERSIGISL
                     IQEYRELPGFGRKYHYAFRSKEDVRSALSGVPSEYISKAEKRACFKLYPSNKQLGIAPP
                     PQMGFQFNACRLTSAVMRRLNKRDRLLYRCRH"
                     /name="hypothetical protein"
     gene            3537..3830
                     /locus_tag="IMP23_RS00025"
                     /old_locus_tag="IMP23_00025"
     CDS             3537..3830
                     /locus_tag="IMP23_RS00025"
                     /old_locus_tag="IMP23_00025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001685281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_195714401.1"
                     /translation="MSFIKNHFDGRDLPLIEAQKQFKKEIDLTVEEIINELPEMLRPII
                     AERAEELVENAPDALKKPDSVTGDVMASAKAVYMRLLFSVSNQLGHGANWLK"
                     /name="hypothetical protein"
     gene            3852..4097
                     /locus_tag="IMP23_RS00030"
                     /old_locus_tag="IMP23_00030"
     CDS             3852..4097
                     /locus_tag="IMP23_RS00030"
                     /old_locus_tag="IMP23_00030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001410737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_195714393.1"
                     /translation="MTFDELEAKGAELVEKRNKATGSERELLNKEIASLRQAQLKALAE
                     EITAALKLAIPEMVSIAIKSSKVNKAIEDLRGEING"
                     /name="hypothetical protein"
     gene            4090..4518
                     /locus_tag="IMP23_RS00035"
                     /old_locus_tag="IMP23_00035"
     CDS             4090..4518
                     /locus_tag="IMP23_RS00035"
                     /old_locus_tag="IMP23_00035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001685282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_195714394.1"
                     /translation="MDEKIKALLNQVRILEEEANKHSAGNERAELFRAAGRIKNQVATL
                     LNQCGKSEPGSKVPDPNAYFSAQLPEGFENYFHGDYLTPEMESYFNNLTPEMESYFSVP
                     KIQNFDNAPSAKPEPAKTQEFDDWELRRRILNAIGIPC"
                     /name="hypothetical protein"
     gene            4578..5075
                     /locus_tag="IMP23_RS00040"
                     /old_locus_tag="IMP23_00040"
     CDS             4578..5075
                     /locus_tag="IMP23_RS00040"
                     /old_locus_tag="IMP23_00040"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_251122126.1"
                     /translation="MNKIEESRIPGIAQGEIVKGIISRGERITNMTPEERAAAREKARE
                     IQNQRHAEWRANADKLKTDFSDKGYWHRAARHYGVTMPGHHVPGTYIPTIRKAMKKMGL
                     LKPGEGFTKAFRETIDEDYRTFVANNPDWPAFAIIGLLLECALERDGIDHLPADRRQIV
                     IR"
                     /name="hypothetical protein"
     gene            5102..5365
                     /locus_tag="IMP23_RS00045"
                     /old_locus_tag="IMP23_00045"
     CDS             5102..5365
                     /locus_tag="IMP23_RS00045"
                     /old_locus_tag="IMP23_00045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000880419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_251123401.1"
                     /translation="MLHNMNRAISIRVAELKAAPTAQQPNKRASLRCFAELVLNTIAGL
                     LRSKEGNTMSPDEKVFYRRCQLVARDIHAQLESVSADIDADI"
                     /name="hypothetical protein"
     gene            5376..5627
                     /locus_tag="IMP23_RS00050"
                     /old_locus_tag="IMP23_00050"
     CDS             5376..5627
                     /locus_tag="IMP23_RS00050"
                     /old_locus_tag="IMP23_00050"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_195714396.1"
                     /translation="MADRGRPTLYSKELEKEICIHALEGMSMRQIAKQPGMPSFRTLLR
                     WTKRYPDFRQTLRECLWLGYHDAVIDRFNQQHPDLVGY"
                     /name="hypothetical protein"
     gene            6335..6961
                     /locus_tag="IMP23_RS00055"
                     /old_locus_tag="IMP23_00055"
     CDS             6335..6961
                     /locus_tag="IMP23_RS00055"
                     /old_locus_tag="IMP23_00055"
                     /inference="COORDINATES: protein motif:HMM:NF012464.2"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0000150 - DNA strand exchange activity [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="WP_195714397.1"
                     /translation="MANAAYIRVSTIAQDTSRQREAIANTGLKFEKIVEEKRSGKDLDR
                     PALRELCGWLREGDDLYVASIDRLGRKLTDVMNVVDELRQKGVRVHFLKEGQIIGGEMN
                     AVQEFFIQLMAAAAQMERSFMLERQKEANEARKAAGKPIGRGLAPKIKQQLPYILKDME
                     QRKESMSDLCRKYKVARSTMYRIRDNAIRDGKLPEIVKVKKVVIN"
                     /name="recombinase family protein"
     gene            7188..7883
                     /locus_tag="IMP23_RS24330"
     CDS             7188..7883
                     /locus_tag="IMP23_RS24330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001521010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="WP_251122127.1"
                     /translation="MEYMDWVDLGMKKRDRKMTKADVGKAMGFSGNGGINHKFNRSRDT
                     TNANADDVLKMLAVVGWQAIIFDSNGKIVMGTAHVDSPEGMEQKFIKRCEELSKENEAL
                     KAEIAQLKAKAVPTVDGTDVTADVTADVTADVTADVTPRVRRNPKPQLFTIADLKLALK
                     AKGGVFEGSLNQLGDLLAVEWCNIDSRADETLKPSKVVVCRLLKAAVEAGVVQVDADKP
                     QGPKTIVLL"
                     /name="XRE family transcriptional regulator"
ORIGIN
        1 agagtgtttt aatggcagta tcgtagaaag cataaagccc gggggcgtcc gggctatcaa
       61 tcaacaaaca ggagaaccag atcatgatta agaaatcgaa cgtggcgctt gttgagaact
      121 caagaatagc agtcaaagca acaaatttca acaaacaaga cgctatacct gacggctggc
      181 aaaagttcgg aaacgtaccg ccagatgctg ttattaccat gcttggaacg gtaaaagagt
      241 ttacatatga cagaaaagta tggcgtaaga caccggacgg cactggttac atgtggcacc
      301 cagcaaatca ccaacaagag caagcgcaga acgacgcagg aacgcaaaac atatactgct
      361 cttacgtttg ccttggtgct ggttataaag cctttaaacg agcatttgct gcgtatgagg
      421 gcaaagataa aacgtcaata tgggctacgt tgagcatcat ggcagcaaac gcacgcaatg
      481 ataacggcgc gtggatcgtc aaagtcagcc agaaggcaat tggcgatgaa ttaaacaaag
      541 accgcaaagc tatagggaga tatatcaagc agcttgttgg cgcgggatta atcttcccta
      601 tcggcagata tatccctggg caggatggca gacagtatcg aatttgctat caggtggagg
      661 acgcgaaatg agtgcggtaa ttagtttaag cgaagtacgc aggcgcaaag agacggcaaa
      721 ggaggccgag attgccagca actggatgtg ggtgctgatg ctccctcttt ccgacaggta
      781 tatcgaagtg ggcgaagaca cctactggct ctatgttggc gacaatgatg gcagcgacaa
      841 gagcgattgg atctggacga gacacagcga attgtccagg gatgagtttt tgtggctaat
      901 caatcgtggc gttggctatt tccttccgcc ggacgggtgc gaaatggaag tttgctacaa
      961 cgtcgagtgg ttcagtttct caagagtctg aacacaaaaa cacagacgcg gggcggcttc
     1021 ggtcgccttt tttgttgact tatccacaat gtcccaccac gcctcacccc cttaatttcc
     1081 atgtcccacc acacaccacc ccatgtccca ccacacacca ccccatgtcc caccacacac
     1141 caccccatgt cccaccacac accaccccat gtcccaccag acacccacat tatatgagtt
     1201 atatgatttt ataagatcag taagagtgta agaagcgcgt gcgcgcgcga gggatttact
     1261 gttttgtgga taacttccaa atcaacatcg tgcccatttg ctggaaagta tcttcaacat
     1321 cctgcctgac gaatgagatc gagatagctg atagttccca ttggcttagc aggagggaga
     1381 cggcgcttcg cttgccccca cgtcccttgc caggcgggta tctctgttcg gcccggtctg
     1441 ccctacaacc tgtagacacc taacctccgt gcggcagcct tttatcgctt cgctcaataa
     1501 aatcggtaga ccgttcggtt tagacaaaaa gtgctgaacg tattacgcat cacaaaaaat
     1561 ggctgtaagg tatttacaga tgccaatttt tctgaatgga aaacggctag atattaacca
     1621 gtgtcgaagc ccacacttaa aatttggcga gtacgtgtcg ggccagcacg aaaacatggc
     1681 gtcacccagc aaatcagaga gtccactggg ctggttaaaa aaatctaacg gacggtaaca
     1741 gtaaccgggc ggaagtaatg ggatccgaac gcagccgcgc cagaggctgg ttgaaacggg
     1801 tttatgcggt tgcccccacc gtagtgaagg gattggggcg aggccggtca gggcgctggc
     1861 tacagggaca gaaacagaaa gccacccgat attcccggcg aacttactta acaggcaatg
     1921 acttctccgc accccattca aagaccggga tctgcccatt aatccctacc agataatagt
     1981 cccaacaaat tttgcgtttt cctagatacc cttccccgcc aatttttcga attgagacat
     2041 acaaaacagc ccgcaaggcc tgattctacc gtcgggattt gtctgcccca ttcagcatgc
     2101 ttgtgggaat aggtccgatc gtcgtttcta ctgcggctcc tgaggtctga aacgcgctga
     2161 agccatccag ggtatacaaa cctatgccca aaaattttgt taatcctgag gccatttgtc
     2221 tgctgaccag acgcgtatcc gttaatgcga aatcattaaa tttttcgcat tgccagaaac
     2281 tgagtgaaaa tggatgcccc tgaggcgcgc tcattcgcat tctgacgagc taatctccag
     2341 acctatggaa acgtaccact ttcaaaaatt agcgcgttgt aggtaccatt cctttcgaat
     2401 atgggccatt caggatagtg atctgtggtt ggtcggcagg agacggattg ggtatggccc
     2461 tgattagcca ctaaggatga gaatcgttat cagctctgga taagcggata aacgggcgcg
     2521 cgattataag ccaactttgt acggatgtaa acaagctgat acaatattag tcagatggtg
     2581 ggaattgtgc ctatcatcgg gagaacagat taatacttct tggccttaat cccgctttcc
     2641 gtattggtta gcgggttttt ttgttggagg caatatgaag ccgaaattat tgagagtaga
     2701 tcaggttctt gagctgctta atatcagccg tgggaagcta tacagacttc taaaaaatga
     2761 accattattc ccgcgcccca ttaaacttgg cggcatgctt tatttcatac cagaagaaat
     2821 cgacgagttt attaatctac agcggcgggt ggaaagataa atgagcaact taaacgacat
     2881 caagtctatt gactctgtga cgcgcattac ctttattgag ggaacgataa atactgcgtt
     2941 attgtatgaa gccgctccgg ttaatttaac tgtacgggtg cccgttaatt ttcttgaacg
     3001 tcgccacgac actaaggaag atggatttat tcgcgcaata atgtttatgg cagcagctgg
     3061 cccgctgaaa tgtggtggcg gtaaaaacga atccgatctt aattacgtca tgggcgttta
     3121 ttacggtcgc aacattaaga cagatattga gcggtcgata ggtatttctt taatccagga
     3181 atatcgcgag ctgccaggtt ttggacgcaa gtaccattat gctttccgta gcaaagaaga
     3241 tgttcgctca gcactgtcag gcgtgccgtc tgaatatatc agcaaggccg agaagcgcgc
     3301 ttgctttaag ctgtacccaa gcaacaagca gttaggcatc gcgccgccac cgcagatggg
     3361 ttttcagttc aacgcctgcc gtctgaccag cgccgtgatg cgcagattaa ataaacgcga
     3421 tcgccttctc taccgctgcc gacattaaaa aatagggggg caactaccaa ccagaaattc
     3481 tatttttcgc gtagcaacac cattctatag aaagctaatt aattggagat aagaaaatgt
     3541 cctttattaa aaatcacttt gatggccgcg acttaccgct tattgaagca cagaaacagt
     3601 ttaaaaaaga gattgatttg accgttgaag aaattattaa cgagctgcct gaaatgctcc
     3661 gcccgattat cgctgagcgg gcagaagaac ttgtcgaaaa cgcgccggac gcacttaaaa
     3721 aaccagattc tgtaacggga gacgtaatgg cgagtgcgaa agccgtatat atgagattgt
     3781 tattttctgt tagcaaccaa ttgggtcatg gtgcgaattg gcttaaataa ctatgaacgg
     3841 gagacataat tatgacattc gacgaactag aagcaaaagg cgctgaatta gttgagaagc
     3901 gtaataaagc aaccgggagc gaacgtgagc tactaaataa ggagatcgca agtcttcgcc
     3961 aggcacaatt aaaagcgctg gcagaagaaa tcacggcggc tttaaaactg gctattccgg
     4021 aaatggttag catcgccatc aagtccagca aggtaaataa agcgatcgaa gatctgagag
     4081 gtgaaatcaa tggatgagaa aatcaaagcg ctattaaatc aggtgcggat ccttgaagaa
     4141 gaagcaaata aacattctgc tggcaatgaa cgcgcagaat tgtttagagc tgccggacgt
     4201 attaagaacc aggtggcaac gctattaaat cagtgtggta aaagcgaacc gggaagtaaa
     4261 gtaccagatc cgaacgcgta tttctccgca cagctgccgg aaggcttcga aaattacttc
     4321 catggcgact acctgactcc ggaaatggaa tcatatttca acaaccttac gccggaaatg
     4381 gagtcatatt tttccgtgcc gaagatccag aacttcgaca acgcgccttc cgctaaaccg
     4441 gagccagcaa aaacccagga attcgatgac tgggaactgc gccgccggat actcaacgcg
     4501 attggcatcc cgtgctaatg gcagaaagaa gccccgtctt gcgcggggca aacaatgcga
     4561 aacgaatggt gatcaagatg aacaaaatcg aagaaagcag aatccctgga atcgcccaag
     4621 gcgaaattgt taaggggatt atatcgcgcg gagaacgcat cacaaacatg acgcctgagg
     4681 aacgcgccgc cgccagggaa aaggccagag agatacaaaa ccagcggcac gctgaatggc
     4741 gggctaatgc cgacaagctg aaaaccgact tttccgacaa aggatactgg caccgcgcag
     4801 ccagacacta cggtgtaacc atgccagggc atcacgtacc tggcacatac atcccgacga
     4861 tccgaaaagc gatgaagaag atgggtctgt taaaaccagg agaaggcttt acgaaggcat
     4921 tcagagaaac cattgatgaa gactaccgga catttgttgc caacaaccct gattggcctg
     4981 cgttcgcaat tattggtctg ctactggagt gcgcgctgga aagagatggg attgaccatc
     5041 ttcctgctga ccgccgccag atagtcattc gctaaggaga aaaaccatgc tttgcctcaa
     5101 gatgcttcac aacatgaacc gagccatttc tatccgcgta gccgaactga aagccgcgcc
     5161 aacagcccaa cagccgaaca agcgcgccag ccttcggtgc tttgccgaac tggtattgaa
     5221 tacgatcgca ggtctgctca ggagcaagga agggaatact atgtctcctg atgagaaagt
     5281 gttctatcgc cgctgccagt tggttgccag ggatatccat gctcagctgg aaagcgtatc
     5341 cgcagacata gacgcagaca tctgaaggag gaatcatggc agatcgcgga agacctaccc
     5401 tgtactcaaa ggagctggag aaagaaattt gtattcatgc gctggaagga atgagtatgc
     5461 gccagatagc gaagcagcca ggaatgcctt cgttccgtac tctgttgcgc tggacgaaac
     5521 gctatccgga tttcaggcag acgctgcgcg agtgtctttg gcttggatac cacgatgcgg
     5581 tcatagacag atttaatcag cagcatccag acctggtcgg ctactaattt ccggacaagt
     5641 acacgcgcgt agccaaatca tcaacgcaac taatatttcc cttctatatt gccgctacaa
     5701 ttaacgcatt ttggggccgt cttttgcgtt caaatggcct caaaagtgcc ttagcgaccc
     5761 cttagcgacc acttagtgcg gtgttaaacg ggtattttcc accattaaat tcgcgaacaa
     5821 ctctgttcaa aaccgcgcca gctgggcttt tgctctatat ggccccctat ttatggcacc
     5881 aaatgagata agacaccgcc acctttagcc aaaaatcaat cagcccgcct tattaatcct
     5941 gccattgttt caatattgct tctcttcttc cggatagttc taccgccgac gccattggcg
     6001 cacaccggcc aggcggcctt tcttttgttc cagaccagaa atttctggtc aacccaagtc
     6061 attgattctt tcacgaacct gcgccgcagg ccactggacc atcccgccac aagcgcccta
     6121 ctcgaaaaat ccctcacaac tacccacagg tttttctatt tttcacgtac acgcgcctga
     6181 agcacccttc ccacatcctt gatatttcgc acaccctgcc aaaagcgtga tctgcgacaa
     6241 aatttgtact ttttgccgtt gccaggtggt ctatatggga ataatctcac aatcaaagag
     6301 aggtattggt atgtgggaaa tggaggaaag tatcatggct aacgcagctt atattcgtgt
     6361 ttctactatc gctcaggata catcgcgcca acgcgaggct atcgctaaca ctggcctgaa
     6421 gtttgagaaa atcgttgaag agaaaaggtc aggtaaagac ctcgatcgcc cggcactgcg
     6481 agagctatgc ggctggttac gtgaaggtga cgacctgtac gtcgcatcta ttgatagact
     6541 ggggcgcaaa ctgactgacg tgatgaatgt ggttgatgaa ctgcggcaga aaggggttcg
     6601 ggtccacttt ctgaaagaag ggcagatcat cggtggcgag atgaacgcgg ttcaggagtt
     6661 cttcatccag ctgatggcag cagccgcgca gatggagcgc agttttatgc tggagcgcca
     6721 gaaagaggca aacgaggcgc ggaaggcggc agggaaaccg attgggcgtg ggctggcacc
     6781 gaagattaag cagcagctgc cgtacatcct caaggacatg gaacaacgca aggagtccat
     6841 gagcgacctt tgccgcaagt acaaagtggc acgaagcaca atgtaccgga tccgcgacaa
     6901 cgccattcgc gatggtaagc tgccggaaat tgtgaaggtc aaaaaggtcg tcatcaacta
     6961 accaaccacc acaaaggggc cagcaaccac gccagcccct ttaatctccc tcacaatccc
     7021 cgctgacatt atagcaaacc gttgtatcga agctattctc gcgatgtaac agcgccgggt
     7081 tttttgtgat ctgtgtcaac atgccagcgt ttgccagtac cgtgaattag tgcgaagtgg
     7141 taccgttgta tcgaaggcaa taactcgatg taacggtggg gtgatttatg gaatatatgg
     7201 attgggtcga tctgggcatg aagaaacgcg atcgcaagat gactaaggca gacgtcggaa
     7261 aggcaatggg cttcagcggt aacggaggca tcaatcacaa gtttaatcgc agccgcgata
     7321 caacaaacgc gaacgctgat gatgtgctaa aaatgctcgc agtagtcggc tggcaagcca
     7381 tcatattcga cagcaacggg aagatcgtca tggggacagc gcacgtagat agccctgaag
     7441 gaatggaaca gaaattcatc aagcgctgtg aagaattgag caaggagaat gaggcactga
     7501 aggctgagat tgcgcagctg aaggcaaaag ccgttccaac ggttgatggc actgatgtaa
     7561 cagccgatgt aacagccgat gtaacagccg atgtaacagc cgatgtaacg cctcgagtgc
     7621 gcaggaatcc gaagccacaa ctatttacca tcgctgacct gaagctggca ctgaaggcca
     7681 aaggcggcgt ttttgaaggt agcctgaacc agcttggcga tctgttagcc gttgagtggt
     7741 gtaacatcga cagccgcgct gatgaaacac tgaaaccgag caaagtcgtt gtttgccgtt
     7801 tgctaaaagc agccgtagaa gctggagtgg ttcaggttga cgctgacaag ccgcaagggc
     7861 caaagacaat tgtgctgttg taacggcgtg cgcgctgatg taacggtcga tgtaacggtc
     7921 gatgtaacgg tagcatcatt tcataaaacg caacgatacg cgtgattgtt gcgaaaagtg
     7981 aaataatcca gtcagaacaa cagtttgcg
//
