LOCUS       NZ_CP062882            11304 bp    DNA     circular CON 16-JUN-2023
DEFINITION  Escherichia coli O157:H16 strain Res13-Lact-PEA12-26 plasmid
            unnamednovel_4, complete sequence.
ACCESSION   NZ_CP062882
VERSION     NZ_CP062882.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN16304178
            Assembly: GCF_015571855.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli O157:H16
  ORGANISM  Escherichia coli O157:H16
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11304)
  AUTHORS   Poulin-Laprade,D., Brouard,J.-S., Gagnon,N., Turcotte,A.,
            Langlois,A., Matte,J.J., Carrillo,C.D., Zaheer,R., McAllister,T.,
            Topp,E. and Talbot,G.
  TITLE     Resistance determinants and their genetic context in bacteria from a
            longitudinal study of pigs reared under conventional and
            antibiotic-free husbandry practices
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11304)
  AUTHORS   Poulin-Laprade,D. and Brouard,J.-S.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) Sciences and Technology, Agriculture and
            Agri-Food Canada, 2000 College, Sherbrooke, Quebec J1M 0C8, Canada
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 16-NOV-2018
            Assembly Method                   :: Unicycler v. 0.4.7
            Assembly Name                     :: PC-37
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 1.00x
            Sequencing Technology :: Oxford Nanopore MinION and Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 06/16/2023 16:33:55
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,186
            CDSs (total)                      :: 5,063
            Genes (coding)                    :: 4,757
            CDSs (with protein)               :: 4,757
            Genes (RNA)                       :: 123
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 94
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 99 of 306
            Pseudo Genes (incomplete)         :: 188 of 306
            Pseudo Genes (internal stop)      :: 62 of 306
            Pseudo Genes (multiple problems)  :: 40 of 306
            Pseudo Genes (short protein)      :: 3 of 306
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP062882.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..11304
                     /organism="Escherichia coli O157:H16"
                     /mol_type="genomic DNA"
                     /strain="Res13-Lact-PEA12-26"
                     /serotype="O157:H16"
                     /isolation_source="feces"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:930406"
                     /plasmid="unnamednovel_4"
                     /geo_loc_name="Canada: Quebec"
                     /collection_date="2017-04-24"
                     /collected_by="Agriculture and Agri-Food Canada"
     gene            complement(join(10983..11304,1..101))
                     /locus_tag="IMP69_RS25560"
                     /old_locus_tag="IMP69_25565"
     CDS             complement(join(10983..11304,1..101))
                     /locus_tag="IMP69_RS25560"
                     /old_locus_tag="IMP69_25565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001255518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="WP_001255519.1"
                     /translation="MRSAAVRLLLIPLITATIALTGCTPKTSLERHTRHYVYASDDGFD
                     PNFHTQKADTIRMMHPFFQQFRDMGMKDKAAGVSAETAQQRVKEFHSEKFFHSLRSTTT
                     FAGRKYTNGDMPSPEKMKLLADTISAVYLDGYEGRQ"
                     /name="Exc2 family lipoprotein"
     gene            223..501
                     /locus_tag="IMP69_RS25565"
                     /old_locus_tag="IMP69_25570"
     CDS             223..501
                     /locus_tag="IMP69_RS25565"
                     /old_locus_tag="IMP69_25570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000710672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000710675.1"
                     /translation="MKKCVPVLLFSVLSASVASHADSNVFQQKAEKTKNGIVADVNARQ
                     KMFSNRLKIQLQIDELRAELAGTRDAQKREDLRKKLESLERQKKSLR"
                     /name="hypothetical protein"
     gene            complement(493..783)
                     /locus_tag="IMP69_RS25570"
                     /old_locus_tag="IMP69_25575"
     CDS             complement(493..783)
                     /locus_tag="IMP69_RS25570"
                     /old_locus_tag="IMP69_25575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001672407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000047032.1"
                     /translation="MSKSPRHKLNKTDKRLLDTLVAAGYEHDKARDLIQKQVYTLTLAD
                     QRHVVSEISNGVNPTQAYSAVYQARRIRLARKYLNGKKVMEETGENTPPSA"
                     /name="hypothetical protein"
     gene            1028..>1468
                     /gene="cta"
                     /locus_tag="IMP69_RS25575"
                     /old_locus_tag="IMP69_25580"
                     /pseudo=""
     CDS             1028..>1468
                     /gene="cta"
                     /locus_tag="IMP69_RS25575"
                     /old_locus_tag="IMP69_25580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_159421185.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="pore-forming bacteriocin colicin 10"
                     /name="cta"
     gene            complement(1502..4468)
                     /locus_tag="IMP69_RS25580"
                     /old_locus_tag="IMP69_25585"
     CDS             complement(1502..4468)
                     /locus_tag="IMP69_RS25580"
                     /old_locus_tag="IMP69_25585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003501156.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
                     /protein_id="WP_000008826.1"
                     /translation="MSFLDAKSYDTLFGLPELYEDALPRFSLSCEDIAFARTHRRSHNR
                     LGFAIQLALVRDLGRTLRAGEIPPEAVITVVAEQLDIDPAVFTLYAQREETRREHAREI
                     VSALELRSVRSSDYRMLIIAATREAATTEKGLPITRAVINKLKDQKLLAPAPAQLIRLA
                     MAGRAAARRLSYRELIRDLKPSSISALEQLLTERVGEKSILGWIAEAPEGVKLKNLKGL
                     IARLEVLRTAAISDEQRKRIHVNRYGIIARDSRVLHAREIQRMASERCHATLVAFVIEK
                     QAAITDLAIDMFCKLIGSTRRRAEISQKERRLKAAEVLEVVAQDHIKLGRALLAARDGN
                     TDIESVITASLGWEGLIASMEAAASVASSHHCSELDELIGRQKSLRKLGRLIFSAFSFR
                     AFRPDDSVLLAIGYLQAFYHGRKLPKQIPLDFMSHKWRRRVIHGENTDLQAWEVAVFVH
                     LRERLKTGDIWVDGSRAWRSFEDYLLPHHVFGQMRAESRLGLAIPDNFAEWRAERTTTL
                     NAKLKALAKAASENAIPDAELSSRGLSVSPIREEESDKIKALSRKLYMLVPRIRITSLL
                     AEVHSWTSFLDCFTHYRTGEQAEDEAALMAAILADATNAGAERMAQSSRGVTIHQMMVM
                     MDRHLRTETYASATSVLVDAQQEHPFAAIWGDGHVSSSDGQFFPAGGRGEASLEYNAKY
                     GKRPGASIYGFLSNRFASFFSRMIQASESEAPYVLDGLLHNESSVVIHEHATDTAGATE
                     TTFSMFHSFGYRLIPRIRNLGSRRLYVISPSEEYKPLDALIAGKAKMEVIEQHWDEVLR
                     LKASIGAGLVPPSVILKKLAASPRQNRLNLALREMGRIERSIFICDWLLDTRLRRRSHA
                     ILNKGESRHALARAIFLHQLGEFRNRAAETMAHRASGLNLVVNAIILWNTVYLSRAVDY
                     IRSQGIIIPEELLSSVSPLPWGHISLTGDYLWNEIDHPLERYRPLRTNRFNPKNFTFP"
                     /name="Tn3 family transposase"
     gene            complement(4449..5072)
                     /locus_tag="IMP69_RS25585"
                     /old_locus_tag="IMP69_25590"
     CDS             complement(4449..5072)
                     /locus_tag="IMP69_RS25585"
                     /old_locus_tag="IMP69_25590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000904945.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0000150 - DNA strand exchange activity [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="WP_000904945.1"
                     /translation="MLIGYMRVSSNDERQSVSLQRDALIAAGVDPRHLHQDHASGARDN
                     RPGLKSCLAELREGDVLIVWKLDRLGRSLSHLLRIIEDLKIRGIAFRSLTESIDTTTPH
                     GSFLFNLFGTLAEYERTLITERVNAGLAAALRRGRKGGRPPAIDAEKVDQILAALEGGA
                     SKASVCRAFSVPRSTLTDTLRRIGWAGPGGRNLTTDQSNVISRR"
                     /name="recombinase family protein"
     gene            <5256..6293
                     /locus_tag="IMP69_RS26005"
                     /pseudo=""
     CDS             <5256..6293
                     /locus_tag="IMP69_RS26005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_159421185.1"
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA];
                     GO:0050829 - defense response to Gram-negative bacterium
                     [Evidence IEA]"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like pore-forming protein"
                     /name="colicin-like pore-forming protein"
     gene            complement(6290..6580)
                     /gene="cki"
                     /locus_tag="IMP69_RS25595"
                     /old_locus_tag="IMP69_25600"
     CDS             complement(6290..6580)
                     /gene="cki"
                     /locus_tag="IMP69_RS25595"
                     /old_locus_tag="IMP69_25600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011264161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin K immunity protein"
                     /protein_id="WP_071528484.1"
                     /translation="MHLKYYLHNLPESLIPWILILIFNDHDNTPLLFVFISSIHVLLYP
                     YSKLTISRYIKENTKFKKEPWYLCKLSALFYLLMAIPVGLPSFIYYSLKRN"
                     /name="cki"
     gene            6610..6741
                     /locus_tag="IMP69_RS25600"
                     /old_locus_tag="IMP69_25605"
     CDS             6610..6741
                     /locus_tag="IMP69_RS25600"
                     /old_locus_tag="IMP69_25605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_072023513.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_077521118.1"
                     /translation="MKIIIFLITILFLSGCQANYIRDVQGGTVAPSSSSELTGIAVQ"
                     /name="colicin release lysis protein"
     gene            complement(7800..7994)
                     /locus_tag="IMP69_RS25605"
                     /old_locus_tag="IMP69_25610"
     CDS             complement(7800..7994)
                     /locus_tag="IMP69_RS25605"
                     /old_locus_tag="IMP69_25610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_001041629.1"
                     /translation="MNKQQQTALNMARFIKSQSLTLLEKLDALDADEQAAMCERLHELA
                     EELGNSIQTHFEAQYGTEP"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            8617..8811
                     /locus_tag="IMP69_RS25610"
                     /old_locus_tag="IMP69_25615"
     CDS             8617..8811
                     /locus_tag="IMP69_RS25610"
                     /old_locus_tag="IMP69_25615"
                     /inference="COORDINATES: protein motif:HMM:NF033650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANR family transcriptional regulator"
                     /protein_id="WP_001367294.1"
                     /translation="MNQDKAIYLENKYFSPANEAVRLEREGDYIGAWKKWNDALHYAKG
                     ENIGWCIARAEYCKKTGRF"
                     /name="ANR family transcriptional regulator"
     Typing          7633..7720
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="94.382"
                     /coverage="100.0"
                     /evalue="1.5000000000000001e-30"
     Typing          complement(9631..9709)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="97.5"
                     /coverage="87.0"
                     /evalue="1.57e-30"
     Typing          complement(6843..7408)
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="80.656"
                     /coverage="97.0"
                     /evalue="2.57e-116"
ORIGIN
        1 cgggtatgtc gttccaggct ggtctttggt gtgcatcctg taagagctat tgttgctgtt
       61 atcagaggta tcagaagcag cctgacagcg gcagatctca taaaaatctc cttgcgtttt
      121 taatcttgat actcagggag ttatatctcc gaagagtatc tcttatcata cgtcggttta
      181 ctcccaatgt gatttgatgg tcaatgagtc aggaataatc ccgtgaaaaa atgtgtgcca
      241 gtcctgcttt ttagtgttct ttctgcttcg gttgccagtc atgcggacag caatgttttt
      301 caacaaaagg ccgagaagac aaaaaacggc attgttgcgg atgttaatgc cagacagaaa
      361 atgttcagta acagactgaa gatacaactt cagatcgatg agttgagagc agagctggct
      421 ggcaccagag atgcccaaaa aagagaggat ttgcgtaaaa aactggagtc tctggagcgg
      481 caaaagaaat ccttacgctg atgggggcgt attttccccg gtttcttcca taaccttttt
      541 accgttcaga tatttacggg cgaggcgaat gcgtcttgcc tggtataccg ccgagtaagc
      601 ctgggtggga ttcacaccgt tactgatttc actgaccaca tgacgctgat cagccagtgt
      661 cagcgtgtaa acctgtttct ggatgaggtc acgggctttg tcatgctcat atccggcagc
      721 aaccagggtg tcgagaagtc gcttgtctgt tttgttcagt ttatggcggg gagatttgct
      781 cacatcgttc tccgtttttt catgatccaa cagttttata tctcatcctg atttgctccg
      841 gtgttctgtt tcgacgttct caatgtctgc ctccttgtag tgtgatcttt attgcaaaaa
      901 tgaaaatcca tgttcttgac atgggcaatg ctgagtagta ggttttaact gtacataaaa
      961 ccagtggtta tatgtacagt atattgtttt taatttattg ttttaattat caaagaggaa
     1021 tttctttatg gataaagtca ctgataattc tccagatgtg gagagcacag aatctactga
     1081 ggggtcattc ccaaccgttg gggttgatac tggcgatacg attacagcga cgcttgcaac
     1141 tggaactgaa aatgttggtg gaggcggtgg agcatttggt ggggccagtg aaagttctgc
     1201 tgcgatacat gcaaccgcta aatggtctac cgcgcagttg aaaaaacatc aggctgaaca
     1261 ggctgcccgt gctgctgcgg ctgaggcagc attggcaaaa gcgaaatctc agcgtgatgc
     1321 cctgactcaa cgtctcaaag atattgttaa tgacgcttta cgtgctaatg ccgcccgtag
     1381 tccatcagta actgaccttg ctcatgccaa taatatggca atgcaggcag aggctgagcg
     1441 tttgcgcctt gcgaaggcag agcaaaaagg ggttccgtgg gcatttctgc gataacacac
     1501 gctaaggaaa agtgaagttc tttggattga agcgattagt acgaagtggc ctgtatcgtt
     1561 caaggggatg gtcaatttcg ttccacaggt aatctccagt gagcgagata tgaccccatg
     1621 gtagtggtga tacactggac aagagctctt ccggtataat gatcccctgg ctgcgaatgt
     1681 aatcaacagc ccgactgaga tagaccgtgt tccacagaat tattgcatta acaacaaggt
     1741 tgaggcccga tgccctgtga gccattgtct cggcagccct gttccggaat tcgcccagtt
     1801 ggtgaagaaa aatagcgcgg gcaagtgcat ggcggctttc gcctttgttg aggattgcgt
     1861 gtgatctgcg acgtaaccga gtgtcaagca accagtcaca gataaaaatt gagcgttcaa
     1921 tacgtcccat ttcacgcagg gccagattga gcctgttttg tctcggagat gctgccagct
     1981 ttttcagtat taccgatggt ggtaccagac cagcgccgat cgaggcctta agccgcagaa
     2041 cttcatccca gtgctgttct attacttcca ttttggcctt tccggcgatc agggcatcaa
     2101 gtggtttata ttcctctgag ggggaaataa cgtacaacct gcgactgccg aggttgcgaa
     2161 tacggggtat gagtctgtaa ccaaaagagt gaaacattga aaatgttgtt tcagtcgcac
     2221 cggctgtatc ggtggcatgc tcatgaatta ccacagaact ttcgttatga agtaagccgt
     2281 caagtacgta gggagcctcg ctttcagagg cctgaatcat tctggaaaaa aaggatgcaa
     2341 aacggttcga taaaaaacca taaatcgacg cgccaggtcg tttgccgtat ttggcgttat
     2401 attcgaggct ggcttctcct cgtccacccg caggaaaaaa ctgcccgtcc gaggatgaca
     2461 catgcccatc cccccatata gcagcaaaag gatgttcctg ttgcgcatcg accagaactg
     2521 atgtggcaga cgcataggtt tcagtgcgta aatgacgatc catcattacc atcatctggt
     2581 gaatggtgac accccgggaa ctctgcgcca tccgttctgc tcccgcattc gtcgcatcgg
     2641 caaggatcgc agccataagt gcagcttcat cttccgcctg ttccccggtg cgataatggg
     2701 tgaaacaatc aagaaaactg gtccagctat gaacttctgc cagcaaactg gtaattctga
     2761 tgcgtggtac cagcatgtag agtttacggc tgagagcctt aattttgtcg ctctcttctt
     2821 cgcgaatcgg agacacagac aaccctctgc tggaaagttc tgcatcgggt atggcattct
     2881 ccgaagcagc ttttgccagc gctttcagtt ttgcgttgag tgtggtggtt cgctcagccc
     2941 gccattcagc aaagttgtcc ggaatggcaa gtcccagccg ggattctgcc cgcatctgcc
     3001 cgaaaacatg gtgaggaaga aggtagtcct caaaactacg ccaggcccga ctaccatcaa
     3061 cccatatgtc acctgttttc aggcgttccc gcaaatgaac gaatacggcg acttcccacg
     3121 cctgcagatc ggtattttct ccatggatga cacgacgtcg ccatttatgg gacataaaat
     3181 cgagcggaat ttgtttgggt aattttcggc catgatagaa cgcctgcaaa tacccaatcg
     3241 ccaacaaaac gctatcatcc gggcgaaaag ctctgaaaga aaaagcgctg aaaatgagtc
     3301 gtccaagctt acgtaatgat ttttgtcgtc cgataagttc atccagttca gaacagtgat
     3361 gggaagatgc caccgaagct gcagcctcca tactggcaat caaaccttcc cagccgagtg
     3421 atgctgttat tacagattca atatcagtat taccgtcacg tgctgccagg agagcccgtc
     3481 ccaacttaat atgatcctga gctacgactt caagtacttc agcggctttc agacgacgct
     3541 ctttctggct tatctctgct ctgcgcctcg tgctgccaat caatttgcag aacatatcaa
     3601 ttgccagatc tgtaattgct gcctgctttt caataacaaa ggctaccagc gtggcatgac
     3661 agcgttcgga tgccattcgt tgtatttcac gcgcatgtaa aacgcgagaa tcccgggcaa
     3721 taataccgta acggtttaca tgaatccgtt tacgttgctc atcggagatt gccgctgttc
     3781 gtaatacctc cagcctggca attaacccct taagattctt gagtttcacg ccttccggcg
     3841 cttcagctat ccagcctaaa atgctctttt ctccgactcg ttcggtaagc aactgctcga
     3901 gagcgctaat ggatgaaggt ttcagatccc tgatgagttc ccgataagaa aggcgacggg
     3961 cagccgcccg gcccgccatt gccagacgga tgagttgtgc cggagcaggg gcaagtaact
     4021 tctggtcctt cagcttattg ataactgctc tggtgatcgg cagtcctttt tcggtggttg
     4081 ctgcttcgcg tgtagccgcg ataatgagca ttctgtaatc gctactccgg acggaacgaa
     4141 gctccaaagc cgaaactatt tcgcgggcat gttcccggcg ggtttcctca cgttgtgcgt
     4201 aaagtgtaaa aacagcaggg tcaatatcca gttgttccgc aacaacggtg ataacagcct
     4261 ccgggggaat ctctcctgca cgcagtgtac gaccgagatc acggacaagt gcaagctgaa
     4321 tggcaaaacc cagacggtta tgagatcgac gatgagttct ggcgaatgca atatcctcgc
     4381 aggagagcga aaaccgggga agtgcatcct cataaagttc aggtaatcca aaaagcgtat
     4441 cgtacgattt agcgtcgaga aatgacattg ctctgatccg ttgtcagatt acgcccccca
     4501 ggtccggccc agccaattcg tcgtagagta tcggtgagcg ttgagcgtgg cacactgaat
     4561 gcgcgacata ctgacgcttt actggcacct ccttccagag ctgcgaggat ttgatcaacc
     4621 ttttcagcat ctatcgccgg tggccttccg ccttttctcc cccgccggag agcagcagcc
     4681 agaccagcat taacacgttc tgtgataagt gttctttcgt actccgcgag cgtgccaaaa
     4741 aggttaaaga ggaacgagcc gtgtggcgtt gttgtatcaa tagattcagt caatgatcgg
     4801 aaagcaatac cacgaatttt caggtcttca ataattcgga gcaaatgtga gagtgaacga
     4861 ccaagtcgat ccagtttcca gactatcagt acatcacctt cgcgaagttc agcgagacag
     4921 gacttaagtc ccgggcgatt atcacgagca cctgaagcat gatcctgatg taaatgccgg
     4981 ggatccactc cagcagcaat caacgcatca cgctgcaaag aaactgactg acgttcatca
     5041 ttgcttgata cccgcatata accaatcaac atgtacgaca aaccttaaaa ttgaagtttc
     5101 cgtacagttt ctcattccga cagggtttgt cacgcaaaaa atcacagatt tatggattca
     5161 attttcttca actgcaggac ctgacggaaa acaagtgatt tccgtcaggg aggaatcgct
     5221 tagcgggtgt tatcgcagaa attcccacgg aaccccaaaa agcccgtgaa gaagctgaag
     5281 cagcagaaaa agcgctccgg gaagcagaac gccaacgtga tgagattgcc cgccaacagg
     5341 ctgaaaccga gcatttgtta gctatggctg aggctgctga ggctgaaaaa aatcgacagg
     5401 attctcttga tgaagagcat cgggctgtgg aagtggcgga gaagaatctg gctgaggcta
     5461 aagctgaact ggcgaaggct gaaagcgatg tacagagtaa gcaagcgatt gtttccagag
     5521 ttgcagggga gcttgaaaac gctcaaaaaa gtgttgatgt gaaggttacc ggatttcctg
     5581 gatggcgtga tgttcagaaa aaactggaga gacaattgca ggataagaag aatgaatatt
     5641 cgtcagtgac aaatgctctt aattctgctg ttagcattag agatgctaaa aaaacagaag
     5701 ttcagaatgc tgagacaaaa ttaaaagaag ctcaggatgc gcttgagaag agtcaggtaa
     5761 aagattctgt tgatactatg gttgggtttt atcaatatat aaccgaacaa tatggagaaa
     5821 aatacgcaag aattgctcag gatttagcgg aaaagtctaa aggaaagaaa attaaaggtg
     5881 ttgatgaagc tttagctgca tttgaacagt ataaaaatgt actggataag aaattcagta
     5941 aggttgatag ggatgctatt tttaattctt tagagtctgt taattatgat gagttatcta
     6001 agaatttaac gaagatatca aagtcgctta aaatcactag tcgagtatct tttctatatg
     6061 atgttggttc tgattttaaa aatgcaatag aaactggtaa ctggcgtcct ttatttgtca
     6121 cgctggagaa aagtgcggta gatgtaggtg ttgcaaaaat tgtggcatta atgtttagtt
     6181 ttattgtcgg cgtgcctctt ggtttctggg ggattgctat tatcacaggt attgtttctt
     6241 cttacatagg ggatgatgag ttgaacaagc ttaatgaatt gctaggtatt taatttctct
     6301 ttagagagta atatatgaaa cttggcaatc ctactgggat tgccattaat aaataaaaca
     6361 atgcagataa cttgcataaa taccagggct cttttttaaa ttttgtattt tctttgatat
     6421 atctagatat ggttaattta gagtatggat atagcaatac atgtattgac gatataaaga
     6481 caaataacaa aggagtgtta tcatggtcgt taaatattaa aataagaatc cagggtataa
     6541 gtgattcagg taaattatgt aggtagtatt ttaaatgcat aagtattttt acttttgttg
     6601 gagagggata tgaaaataat aatttttcta attacaatat tattcttgtc aggatgtcaa
     6661 gcaaactata ttcgtgatgt acaaggagga accgttgcgc catcttcgtc ttctgaactg
     6721 acggggatcg cggttcagta gaaaagatca aaggatctct tgagatcctt tttttctgcg
     6781 cgtaatctgc tgcctgtaaa cgaaaaaacc gcctggggag gcggtttagt caaaggttaa
     6841 gtcagttggg gaacagctta accgggtaac tggcttcagc agagcgcaga taccaaaaac
     6901 ttgtccttct agtgtagtca aaatcaggct gatacttcaa gaactctgtt actaacttat
     6961 aaatctcgct ctgctaaccc ggttaccaat ggctgctgcc agtggcgtta agtcgtgtcc
     7021 tttccgggtt ggacccaaga gaacagttac cggagaaggc gcagcagtcg ggctgaacgg
     7081 ggggttcctg catacagccc ggcccggagc gaactgccta agtcgccccg agataccaga
     7141 agacgtgagc tatgagaaag cgccacgtgt cccgcaaggg agaaaggcgg aaaaggtatc
     7201 cggcaatcga tatggcggga acgtgggggc gcaggaggga gcgattcacc ggaaacggtg
     7261 atgatcttta agccacgtcg ggtttcgcca ccgctggcgg atttcatggt tgagtcggaa
     7321 gactcccaca gatgcaccta aaaagtgtct gttttgtgaa gtccgtcagg ggggcggagc
     7381 ctatggaaaa agcgcctccg gcgcggcctt tcggggtttt cgtttcttgc tcacatgttc
     7441 tttccggcgt tatcccctga ttctgtggat aacactatca ccgcttttga gtgggcagac
     7501 accgctcgcc gcagccgaac gaccgagcgt agcgagtcag tgagcgagga agcggaaaag
     7561 cgcctggacg cgcattttct ccttacgcat ctgtgcggca tttcacaccc ggaacggtgc
     7621 acttttcata caactcgcac tgttgccaca taagtaagcc agtatacact ccgctatcgc
     7681 tacgtgactg ggtcatggct gcgccccgac acccgctaaa acccgctgac gcgccctgac
     7741 gagcttgtct gctggcgaaa acactcacgg acaagccgcg accgctttcg ccagcccctt
     7801 tatggttccg tgccgtactg cgcttcaaag tgtgtctgta tgctgttacc gagttcttcc
     7861 gccagttcgt gcagtcgttc gcacatggcg gcctgttcgt ctgcatccag tgcatccagt
     7921 ttttcgagca acgtcaggct ctggcttttg ataaatcgcg ccatgttgag cgcggtctgc
     7981 tgctgtttgt tcacgttctc ccttctccgt tactggtgtt tacgtccggc tgtgcgtcac
     8041 ctggtcatca cgctaggtga cgtcactgaa aattatcaca caagccccgt cgtgtgtggg
     8101 ctgcgcgaca ttaacacccc gtgacgctta ttttttgctg aatatgtgcg cgattttctg
     8161 tctgcgttgt gtgcagttgt cgtcataaac atgaacgttc cgccggttcc tgatgtgcgt
     8221 cactttgacg ttttgacaaa gtgacgcacg ggaggtgctt gcgcaaaccc gcgaataacg
     8281 gcgtttaccg cgtgattttt ttacatctga caacgtgacc cggcgtgatg gccagttctg
     8341 gccagagacg ttcgcggggt gtcggggcgc agccatgacc gggtggcgtt tgtccggtcg
     8401 tgtgtgcgcg gtccgacaaa tgcacatcct gtcccgtccc gtttttcgct cggtttttat
     8461 tgattaccgg acagacgaac ccggattgcc atattgcgaa aatcacgtta aaacgattac
     8521 gagcgtgcag ttgggctgat atatcaacag tcatgccaat atgtattaac ccgacaggcc
     8581 gctcttttta attccaataa aaatataaat acgagtatga atcaggacaa agcaatttat
     8641 ttagaaaata aatatttttc acccgcaaac gaagctgtca gactggagcg tgaaggcgat
     8701 tacattggag catggaaaaa atggaatgat gcgcttcatt atgccaaagg agaaaatata
     8761 ggttggtgta tagccagagc tgaatattgt aaaaagacag gccggtttta attctggcgg
     8821 ggtttcaaag gggcgccccc tttggcacct cgacgtatca aaatgcacca gcattttgta
     8881 tacagtcggt catgtgcctc caggatttac catcaaaaac gcccctgtag ttaatcctga
     8941 atcaaggtat aactaagata taattagcgt ataactaagg cataactaaa gtataactaa
     9001 cttggtgtgc atataattaa catataacta atgtataact aacggttcac caacggatcg
     9061 catgtagtag acgctacata aaaaatcctg aaatggagga ttcgtcataa agagattctt
     9121 ttttttagcg ttctgatgca gaacagggca aacaccgcaa aaatgatgaa aaactgatta
     9181 accatactgt ctgtattctg aatgaattac gaagcatagc gataaattaa aaaatccccc
     9241 tggattacag ggggaagcca tcagagtttg agtaatgaga aatattattt tggagagagc
     9301 atcacaaatg cgctcttttc caatctgccg taaccatcgg caaaagacaa tcaggacagg
     9361 cgaaaaaatc cccctatatg gcaagggggt gtcaatgagg acatggcaat tgtgagtttt
     9421 gtgtgtgttt tatgtgttgc agtttgcaac gtcagaaatt tgccgtagtt cgtcatgaga
     9481 gacaacaaaa aaatcccccg tgactggtgg aggccagggc aaggggggta gcaaatgcct
     9541 gaataatttt cgtagttttg atatacccat cgttgcaacc gcaacgggat gaatttgccg
     9601 cagttgtgcg cgaaaaacaa tcctgattcc gggacaggat gtgtaatcgt ataaccgcgc
     9661 acgcacggct atgcgattac cacctggtca gggctttgcc ccgacaccct cgacaccgtt
     9721 cccgcgatac gatagaaggg gttctaagcc ggaaacccag aaaattccgt caccaaagct
     9781 agtaatcgac agatttgcat gacgttgaat aggtgacggg ttatgtgacg atatttggtg
     9841 tcgaaatcgt tgtttcagtg acagtcactc aatcgatcgt ttgtgtgaca ctcccgtaaa
     9901 aacttgtcgc agaaaaatcc aggtttccgg ccgggaaccc ctagaacatc agggcgcgtt
     9961 tccgggtaaa cgcatgttaa tgaccgggaa ttcattgatc agggaccgga gacgcgctac
    10021 aaaactcctg cgcgtttctg gcatcaactg accatgggga tggtttggca gtagcagcaa
    10081 gcgccagaaa cgcgctcaaa acgcctctgg cggcaaaacc attgcccgat gcaaaaaagt
    10141 gcgttacaaa gcctctgagc gcgtttctgg cgtctttttt gttgctgtgt cgctatactc
    10201 gttgtcagat tcgcgctcaa tcatcactga ttaatctgcg cttatccttc gactgatcac
    10261 gctggtattt tttgacaacc ggttcccatg aaaacgccag acgggaaccg tcatccgcac
    10321 cctctgacat gctggggatt aaaaatccct gtgccgcaca gccggcgttg cataaaattc
    10381 cgctctgcga tgagtattgt ttaatcaaaa atcccctcgt tttctccaga aaaacgcgag
    10441 tctgttctca tgccccggat gcccacaccc cacgaaaaac caactttttt ctgatttgct
    10501 ttttaaatca tgttgttatg gtttgaaaaa tatattttct tattcagatt tatacaaaag
    10561 cgagtcgtcg gcgactactg gatgattcgg caacgagtcg tcggtgattt atcgatgact
    10621 caacacactg tctccctggt gttcagaaaa agcgagtcgc cagagagtcg cgaggaaaaa
    10681 tactggatga ctcgctgatg gctcccgggg tgaatcgctc acgactcgtc tgtaactact
    10741 gtctgatttc ttctcatcag ggctgaaaac gtccgaaaat gcgccttatg ggaatcaggc
    10801 gcgcccaaac cgatcgccat tcagtttcat gcatagtcat gcagtgccat gaaaacaatc
    10861 ctgacgtatt tttccggttt acccgggggg aaaacatctc tttttgcggt ttttgcgtca
    10921 gaatcgcgct gagcgcgttt ctgcggtgcg tacaattaag ggattatggt aaatgtctcc
    10981 ctttactgcc tgccctcgta gccatcaaga taaaccgcag aaatcgtgtc agccagcagc
    11041 ttcatttttt ccggtgatgg catatcaccg ttcgtgtatt ttctgccagc aaaggttgtt
    11101 gtgctccgga gtgagtgaaa aaatttttct gagtggaatt cttttacacg ttgctgtgcc
    11161 gtttctgctg atactccggc tgctttgtct ttcatcccca tatcccggaa ctgctgaaag
    11221 aacgggtgca tcatacgtat ggtgtctgct ttctgggtgt ggaagttagg atcaaatcca
    11281 tcatctgaag cataaacata atgc
//
