LOCUS       NZ_CP062872             9695 bp    DNA     circular CON 16-JUN-2023
DEFINITION  Escherichia coli strain Res13-Lact-PEA14-20 plasmid unnamed29,
            complete sequence.
ACCESSION   NZ_CP062872
VERSION     NZ_CP062872.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN16304185
            Assembly: GCF_015571755.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9695)
  AUTHORS   Poulin-Laprade,D., Brouard,J.-S., Gagnon,N., Turcotte,A.,
            Langlois,A., Matte,J.J., Carrillo,C.D., Zaheer,R., McAllister,T.,
            Topp,E. and Talbot,G.
  TITLE     Resistance determinants and their genetic context in bacteria from a
            longitudinal study of pigs reared under conventional and
            antibiotic-free husbandry practices
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9695)
  AUTHORS   Poulin-Laprade,D. and Brouard,J.-S.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) Sciences and Technology, Agriculture and
            Agri-Food Canada, 2000 College, Sherbrooke, Quebec J1M 0C8, Canada
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 28-NOV-2018
            Assembly Method                   :: Unicycler v. 0.4.7
            Assembly Name                     :: PC-34
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 1.00x
            Sequencing Technology :: Oxford Nanopore MinION and Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 06/16/2023 13:27:08
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,207
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 4,837
            CDSs (with protein)               :: 4,837
            Genes (RNA)                       :: 123
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 93
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 76 of 247
            Pseudo Genes (incomplete)         :: 161 of 247
            Pseudo Genes (internal stop)      :: 53 of 247
            Pseudo Genes (multiple problems)  :: 42 of 247
            Pseudo Genes (short protein)      :: 4 of 247
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP062872.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..9695
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="Res13-Lact-PEA14-20"
                     /serotype="O-untypeable:H16"
                     /isolation_source="feces"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:562"
                     /plasmid="unnamed29"
                     /geo_loc_name="Canada: Quebec"
                     /collection_date="2017-04-24"
                     /collected_by="Agriculture and Agri-Food Canada"
     gene            join(8244..9695,1..195)
                     /gene="cka"
                     /locus_tag="IMP76_RS24300"
                     /old_locus_tag="IMP76_24295"
     CDS             join(8244..9695,1..195)
                     /gene="cka"
                     /locus_tag="IMP76_RS24300"
                     /old_locus_tag="IMP76_24295"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="pore-forming bacteriocin colicin K"
                     /protein_id="WP_162542215.1"
                     /translation="MAKELSGYGPTAGESMGGTGANLNQQGGNNNSNSGVHWGGGSGHG
                     NNGGQGNSNSSGSTSTVMKTGESYLTPWGDVVINNDGLPVMNGIVMTEENSTLVDNPFG
                     GVSRVLNSLISDMPSLFAESSGNNNNNTASVNTAPTNAQVSDMDKSSKVVGNVINEKQK
                     QKNKIATQISEKQKKIEEMKKVFKHHSYHGITDLERDVDELQKKSNQLDADISKLNSYK
                     NTLQSKIGDVNKQKEAEEKARENAEVAEHETLNEEKQAVAEAEKRLAEVKAELAKAESD
                     VQSKQATVSRVAGELENAQKSVDVKVTGFPGWRDVQKKLQRQLEAKQAEYSAVENELKN
                     AVSFRDGKAAEVKEAEQKLKEAQDALEKSQIKDAVDTMVGFYQYITEQYGEKYAKIAQD
                     LAEKSKGKKIQGVDEALAAFEKYKNVLDKKFSKVDRDAIFNALESVNYDELSKNLTKIS
                     KSLKITSRVSFLYDVGSDFKNAIETGNWRPLFVTLEKSAVDVGVAKIVALMFSFIVGVP
                     LGFWGIAIVTGIVSSYIGDDELSKLNELLGI"
                     /name="cka"
     gene            complement(192..482)
                     /gene="cki"
                     /locus_tag="IMP76_RS24305"
                     /old_locus_tag="IMP76_24300"
     CDS             complement(192..482)
                     /gene="cki"
                     /locus_tag="IMP76_RS24305"
                     /old_locus_tag="IMP76_24300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011264161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin K immunity protein"
                     /protein_id="WP_011264161.1"
                     /translation="MHLKYYLHNLPESLIPWILILIFNDNDNTPLLFIFISSIHVLLYP
                     YSKLTISRYIKENTKLKKEPWYLCKLSALFYLLMAIPVGLPSFIYYTLKRN"
                     /name="cki"
     gene            512..643
                     /locus_tag="IMP76_RS24310"
                     /old_locus_tag="IMP76_24305"
     CDS             512..643
                     /locus_tag="IMP76_RS24310"
                     /old_locus_tag="IMP76_24305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_072023513.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_077521118.1"
                     /translation="MKIIIFLITILFLSGCQANYIRDVQGGTVAPSSSSELTGIAVQ"
                     /name="colicin release lysis protein"
     gene            complement(1649..1840)
                     /locus_tag="IMP76_RS24315"
                     /old_locus_tag="IMP76_24310"
     CDS             complement(1649..1840)
                     /locus_tag="IMP76_RS24315"
                     /old_locus_tag="IMP76_24310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165980.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADDQADICEALHDHA
                     DELYRSCLARFGDDGENL"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            1908..2231
                     /locus_tag="IMP76_RS24320"
                     /old_locus_tag="IMP76_24315"
     CDS             1908..2231
                     /locus_tag="IMP76_RS24320"
                     /old_locus_tag="IMP76_24315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_001444404.1"
                     /translation="MLTIRVTDEEHARLLARCEGKQLASWMRKVCLGAPPSKTSGLPTL
                     APPLLRQFASVGNNLNQIARKINSGQWSGHDRVHVVAALMAIGRELSELRDEVRKQGER
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            2440..>2877
                     /locus_tag="IMP76_RS26080"
                     /pseudo=""
     CDS             2440..>2877
                     /locus_tag="IMP76_RS26080"
                     /inference="COORDINATES: protein motif:HMM:NF015397.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            2909..3469
                     /locus_tag="IMP76_RS24330"
                     /old_locus_tag="IMP76_24325"
     CDS             2909..3469
                     /locus_tag="IMP76_RS24330"
                     /old_locus_tag="IMP76_24325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000067489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_000067492.1"
                     /translation="MSNLLQMGTDFEKKLKERAVSTENMLNSEFRKLEASVDRELNLNG
                     QKIRDAISAHTAAVKEQLETLNSAVNAQFSATENELARRQEELLWKLVKGRILYPSLTA
                     LCVTGGIFLASWGLIQWQESRIAANILAIRDQEETLAKLREKTWGVTYHEGRNGKFLVL
                     PSGVKGENNWTVEKKNAVRLVRE"
                     /name="MbeB family mobilization protein"
     gene            3476..3709
                     /locus_tag="IMP76_RS24335"
                     /old_locus_tag="IMP76_24330"
     CDS             3476..3709
                     /locus_tag="IMP76_RS24335"
                     /old_locus_tag="IMP76_24330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_000137427.1"
                     /translation="MTELEMHLLNALEQLQQDYTQRLKDWESAFTDLQHMFELTRQENG
                     QLREQCDDLSQQVQRLAGQVDHLSRLFITNSR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(4193..4615)
                     /locus_tag="IMP76_RS24340"
                     /old_locus_tag="IMP76_24335"
     CDS             complement(4193..4615)
                     /locus_tag="IMP76_RS24340"
                     /old_locus_tag="IMP76_24335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001255518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="WP_001255518.1"
                     /translation="MRSAAARLLLIPLITATIALTGCTPKTSLERHTRHYVYASDDGFD
                     PNFYTQKADTIRMMLPFFQQFRDMGMKDKAAGVSAETAQQRVKEFHSEKFFHSLRSTTA
                     FAGRKYTNSDMPSPKKMKLMADTISAVYLDGYEGRQ"
                     /name="Exc2 family lipoprotein"
     gene            complement(4677..6248)
                     /locus_tag="IMP76_RS24345"
                     /old_locus_tag="IMP76_24340"
     CDS             complement(4677..6248)
                     /locus_tag="IMP76_RS24345"
                     /old_locus_tag="IMP76_24340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66-like element ISCro1 family transposase"
                     /protein_id="WP_000381395.1"
                     /translation="MDTSLAHENARLRALLQTQQDTIRQMAEYNRLLSQRVAAYASEIN
                     RLKALVAKLQRMQFGKSSEKLRAKTERQIQEAQERISALQEEMAETLGEQYDPVLPSAL
                     RQSSARKPLPASLPRETRVIRPEEECCPACGGELSSLGCDVSEQLELISSAFKVIETQR
                     PKQACCRCDHIVQAPVPSKPIARSYAGAGLLAHVVTGKYADHLPLYRQSEIYRRQGVEL
                     SRATLGRWTGAVAELLEPLYDVLRQYVLMPGKVHADDIPVPVQEPGSGKTRTARLWVYV
                     RDDRNAGSQMPPAVWFAYSPDRKGIHPQNHLAGYSGVLQADAYGGYRALYESGRITEAA
                     CMAHARRKIHDVHARAPTYITTEALQRIGELYAIEAEVRGCSAEQRLAARKARAAPLMQ
                     SLYDWIQQQMKTLSRHSDTAKAFAYLLKQWDALNVYCSNGWVEIDNNIAENALRGVAVG
                     RKNWMFAGSDSGGEHAAVLYSLIGTCRLNNVEPEKWLRYVIEHIQDWPANRVRDLLPWK
                     VDLSSQ"
                     /name="IS66-like element ISCro1 family transposase"
     gene            complement(6268..6615)
                     /gene="tnpB"
                     /locus_tag="IMP76_RS24350"
                     /old_locus_tag="IMP76_24345"
     CDS             complement(6268..6615)
                     /gene="tnpB"
                     /locus_tag="IMP76_RS24350"
                     /old_locus_tag="IMP76_24345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858152.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="WP_000624622.1"
                     /translation="MISLPAGSRIWLVAGITDMRNGFNGLASKVQNVLKDDPFSGHLFI
                     FRGRRGDQIKVLWADSDGLCLFTKRLERGRFVWPVTRDGKVHLTPAQLSMLLEGINWKH
                     PKRTERAGIRI"
                     /name="tnpB"
     gene            complement(6615..7292)
                     /gene="tnpA"
                     /locus_tag="IMP76_RS24355"
                     /old_locus_tag="IMP76_24350"
     CDS             complement(6615..7292)
                     /gene="tnpA"
                     /locus_tag="IMP76_RS24355"
                     /old_locus_tag="IMP76_24350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743492.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0004803 - transposase activity [Evidence IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66-like element accessory protein TnpA"
                     /protein_id="WP_001299364.1"
                     /translation="MSIIFNGHYRMKHRTWITEALRLHFEEHLPRVVAGRRLSVPKSTV
                     CSMFVRFRRAGLSWPLPAGMSEQELDACLYGQFSTVPVVRPESTVISEAPVVKKRPRRP
                     NFPYEFKIALVEQSLQPGACVAQIARENGINDNLLFNWRHQYRKGGLLPSGKNMPALLP
                     VTLTPEPDNKIPAPAQEPEQINTPSDSLCCELVLPAGTLRLKGKLTPALLQTLIREIKG
                     SSH"
                     /name="tnpA"
     gene            7444..7722
                     /locus_tag="IMP76_RS24360"
                     /old_locus_tag="IMP76_24355"
     CDS             7444..7722
                     /locus_tag="IMP76_RS24360"
                     /old_locus_tag="IMP76_24355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000710672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_021565938.1"
                     /translation="MKKCVPVLLFSVLSASVASHADSNVFQQKAEKTKNGIVADVNARQ
                     KIFSNRLKIQLQIDELRAELAGTRDAQKREDLRKKLESLERQKKSLR"
                     /name="hypothetical protein"
     gene            complement(7714..8004)
                     /locus_tag="IMP76_RS24365"
                     /old_locus_tag="IMP76_24360"
     CDS             complement(7714..8004)
                     /locus_tag="IMP76_RS24365"
                     /old_locus_tag="IMP76_24360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001672407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000047032.1"
                     /translation="MSKSPRHKLNKTDKRLLDTLVAAGYEHDKARDLIQKQVYTLTLAD
                     QRHVVSEISNGVNPTQAYSAVYQARRIRLARKYLNGKKVMEETGENTPPSA"
                     /name="hypothetical protein"
     Typing          1477..1564
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="6.86e-39"
     Typing          complement(910..1466)
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.633"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          2222..3816
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.624"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 aactggcgac ctttgtttgt cacgctggag aaaagtgcgg tcgatgtagg tgtggcaaaa
       61 attgtggcat taatgtttag ttttattgtt ggtgtgcctc ttggtttctg ggggattgca
      121 attgtcacag gtattgtttc ttcttacata ggggatgatg agttgagcaa acttaatgag
      181 ttgttaggga tttaatttct ctttagagtg taatatatga aacttggcaa tcctactggg
      241 attgccatta ataaataaaa caatgcagat aacttgcata agtaccaggg ttcttttttt
      301 aactttgtat tttctttgat atatctagat atggttaatt tagagtatgg atatagcaat
      361 acatgtattg atgatataaa tataaataac aaaggagtgt tatcattgtc gttaaatatt
      421 aaaataagaa tccatggtat aagtgattca ggtaaattat gtaggtagta ttttaaatgc
      481 ataagtattt ttacttttgt tggaaagaaa tatgaaaata ataatttttc taattacaat
      541 attattcttg tcaggatgtc aagcaaacta tattcgtgat gtacaaggag gaaccgttgc
      601 accatcttcg tcttctgaac tgacggggat cgcggttcag tagaaaagat caaaggatct
      661 tcttgagatc ctttttttct gcgcgtaatc tgctgcctgt aaacgaaaaa accgcctggg
      721 gaggcggttt agtcgaaggt taagtcagtt ggggactgct taaccgggta actggcttta
      781 gtggagcaca gataccaaat gctgtccttc cagtgtagcc gctataaggc ttcaacttca
      841 agagttctgc gtatctaaat ccactatcca gttaccaatg gctgctgcca gtggcgttaa
      901 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg
      961 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag
     1021 ataccaacag cgtgagctat gagaaagcgc cacgcttccc aaagggagaa aggcggacag
     1081 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagccac cagggggaaa
     1141 cgcctggtat ctttaagtcc tgtcgggttt cgccacctct gacttgagcg tcggtttttg
     1201 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gctgcgtggc cttttccggg
     1261 ttcttgtttt ttgctcacat gtttcttttg gtgttatccc ctgattctgt ggataaccgc
     1321 attaccgtct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcggagcgag
     1381 tcagtgagcg aggaagcgga aaagcgcctg gacgagcatt ttctccttac gcatctgtgc
     1441 ggcatttcac acccggcatg gtgcactttt catacaatcc tcactgatgc caaatgatta
     1501 agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc
     1561 taaaacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct cacaggccag
     1621 ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatcc ccgaagcgtg
     1681 cgaggcagct gcggtaaagc tcgtcagcgt ggtcgtgaag cgcttcgcag atgtcagcct
     1741 ggtcgtcggc gtccagttca ttcagttttt ccagcagggt cagcgtctgg cttctgatga
     1801 agcgggccat gttaagggcg gttttttcct gtttagtcac agttctgttt ctccatgtgt
     1861 ggggagtctg tcggaggttt ttaatgatac cggtgaaacg cgaaaggatg ctcacgatac
     1921 gggtcacgga tgaggaacat gcgcggctgc tggcacgttg tgagggaaaa caacttgcgt
     1981 catggatgcg taaggtctgc ctgggggcac cgccatcgaa aacgtcaggt cttccgacac
     2041 tggcaccgcc gctgttgcgc cagttcgctt cggtcggcaa taacctgaat cagatagccc
     2101 ggaagataaa cagcggacag tggtcggggc atgaccgggt tcatgtggtg gctgcactga
     2161 tggccatcgg acgtgaactg agtgagctga gggatgaggt ccgtaaacag ggagaacggg
     2221 atgatagtta agtttcatgc caggggaaaa ggcggtggca gtggtccggt tgattacctg
     2281 ctgggccggg agcgtaaccg ggaaggggcg agagttctgc ggggtgctcc cgaagaggtg
     2341 cgggaactca tcgatgccac gccgtttgcg aaaaaataca cctcgggggt tctgtctttt
     2401 gcagagcaga ccctgccacc gggagagcgt gaaagggtga tggagagctt tgagcgggta
     2461 ctgatgcccg gtcttgaaaa aaatcagtac agcatcctgt gggtggagca ccaggacaag
     2521 gggcgtcttg agctgaattt tgtcatcccg aatatggagc tggcgagcgg aaaacggctc
     2581 cagccctatt acgaccgagc ggaccgtccg cgaatcaatg cctggcagac actggtgaat
     2641 caccattacg ggctgcacga cccgaacgca ccggagaaca ggcggacact tgtcaccccg
     2701 aataatctgc cgaaagcgaa acaggaggcc gcagaagcga ttacgagggg actggaggct
     2761 ctttaccatg ccggagcgct aaaaacgcgt caggatgtca ctgaggcgct tacagcggca
     2821 ggttttgagg ttgtgaggac aaccaggagc agtatcagta tcgccgaccc ggagggtggg
     2881 cgtaatctca ggctgaaagg ggcgctgtat gagcaatctt ttacaaatgg ggacagactt
     2941 cgagaagaaa ctgaaagagc gtgccgtctc tacagagaac atgctgaaca gcgagttcag
     3001 gaagctcgaa gcgtctgtag acagggaact gaacttaaac gggcagaaaa tcagagacgc
     3061 tatcagcgca cacacagcag cggtgaaaga acaactggag acgctgaaca gtgccgtgaa
     3121 cgcgcagttc agcgccacag agaacgaact ggcccggcgg caggaagagc tgctctggaa
     3181 gctggtcaaa gggcggatac tgtacccgtc gctgacggcg ctttgcgtca cgggcgggat
     3241 ttttctggcg tcatgggggc tgattcagtg gcaggaaagc cggattgcgg cgaacatcct
     3301 ggctatcagg gaccaggaag agacgctggc gaaactccgg gaaaaaacat ggggtgtgac
     3361 gtatcacgag ggcaggaacg ggaaatttct ggtgttgccg tccggggtga agggggaaaa
     3421 caactggacg gtggagaaga agaacgcagt aagactggtc agggagtaag tgagtatgac
     3481 agagctggaa atgcacttac tgaacgcctt agagcaattg cagcaggact atacgcagcg
     3541 gctgaaagac tgggagagcg ccttcacgga tttgcagcat atgttcgagc tgacacgaca
     3601 ggagaacggg caactgaggg aacagtgcga cgacttgagt cagcaggtgc agagattagc
     3661 cgggcaggtg gaccacttga gtcggttatt tatcacgaac agcagataag ggatgaacag
     3721 gaaaggcagg cacgcctgga acgggagcgt gcgcggtcct ggcatgaaga gcaggaacgg
     3781 gaagcacggg tatggcgtgg cccgtcaata gggatgtgac ggtgagttgt tccgggaggt
     3841 tgccagatgg taccggaacg cgttaaaaac gcatccgggc gcgattctgg cgtgtcgttt
     3901 ttgttgctgt gttttgcctc tttgccagat cgcgctcatt cctcagcctc atcccgcgat
     3961 gtctgcgtat gtcatcaact gaaaagatgg aaaaaaaaca gtgtcatgag gtttacgcag
     4021 gcaaaaccag cgttattcac atggctgaat agctatgcat acggttaaaa tttatcaggt
     4081 gcgatcgcgg cagcttttcg ggtggtttgt tgccattttt acctgtcagc agccgtgatc
     4141 gcgctgaacg catttcagcg gtgcgtacaa ttaagggatt atggtaaatc ccttactgtc
     4201 tgccctcgta tccatcgaga taaaccgcag aaatggtgtc tgccattagt ttcatttttt
     4261 tcggcgaagg catatcgctg tttgtgtatt ttctgccagc aaaggctgtt gtgctccgga
     4321 gtgagtgaaa aaatttttct gagtggaatt cttttacacg ttgctgtgcc gtttctgctg
     4381 atactccggc tgctttgtct ttcatcccca tatcccggaa ctgctgaaag aacgggagca
     4441 tcatacgtat ggtgtctgct ttctgggtgt agaagttagg atcaaatcca tcatctgaag
     4501 cataaacata atgccgggta tgtcgttcca ggctggtctt tggtgtgcat cctgtaagag
     4561 ctattgttgc tgttatcaga ggtatcagaa gcagcctggc agcggcagat ctcataaaca
     4621 tctccttgcg tttttaatct tgatacgtaa gcgactcgtc agaaccgtat tgatatttac
     4681 tgagagctca gatcaacttt ccagggcaac agatcgcgta cccggtttgc cggccagtcc
     4741 tggatatgtt caatgacgta acgcagccac ttttctggct ccacattgtt cagacggcat
     4801 gtgccgatca gcgagtacaa caccgccgca tgttcaccac cgctgtcgga acccgcgaac
     4861 atccagtttt tccggcctac ggccactccc cgtaaggcgt tctctgcgat gttgttgtcg
     4921 atttccaccc agccattact gcagtacacg ttcagtgcat cccactgttt cagcaggtat
     4981 gcgaacgctt ttgccgtatc tgagtgacgc gacagtgttt tcatctgttg ctgtatccag
     5041 tcatacagtg actgcatcag tggcgcggct ctggcttttc ttgccgccag acgctgttct
     5101 gctgaacagc cccggacctc tgcctcgatg gcatacagtt caccgatacg ctgcagggct
     5161 tccgtggtga tgtaggtggg cgctcttgca tgcacatcgt ggatttttct ccgggcatga
     5221 gccatacacg cggcttccgt tattctgccg gattcgtata acgcccggta accaccgtaa
     5281 gcatcggcct gaagcacacc gctgtaaccg gccaggtgat tttgtggatg gatacctttc
     5341 cggtccggac tgtacgcgaa ccagaccgcc gggggcatct gtgaaccggc gttacggtca
     5401 tcacggacgt agacccacag ccgggctgtc cgggttttac cgctgcccgg ctcctggacc
     5461 gggacgggga tatcatcagc atggacttta ccgggcatca gcacatactg gcgcaggacg
     5521 tcatacagcg gctccagcag ttcagcaaca gcacctgtcc agcgccccag tgtggcacgg
     5581 ctcagctcca ctccctgacg acggtatatt tctgactggc ggtataacgg cagatggtct
     5641 gcatatttcc cggtgacaac atgggccaga agccccgctc cggcataact gcgtgcaatg
     5701 ggttttgaag gtactggtgc ctgcacgata tggtcgcacc ggcaacaggc ctgtttcgga
     5761 cgttgtgttt cgataacctt aaaggcgctg ctgataagct ccagttgctc tgacacatca
     5821 catcccagag aactgagttc accaccacag gcaggacagc attcctcttc cggccggata
     5881 acccgggttt cacggggaag tgaggccggt aacggtttac gggctgaaga ctggcgcagg
     5941 gcggatggca gtaccgggtc atattgctca cccagcgttt ccgccatttc ttcctgaagt
     6001 gcgctgattc gctcctgtgc ttcctgtatc tgccgttcgg tttttgcacg aagtttttct
     6061 gagcttttac cgaactgcat acgttgcagt ttcgcaacca gcgccttcag ccggttgatt
     6121 tcggaagcat aagccgccac ccgctgtgag agcaggcggt tgtattcagc catctggcgg
     6181 atggtgtcct gttgcgtctg caacagtgcc cgcaggcggg cgttctcatg agcaagtgag
     6241 gtgtccatat cctcacttta caacgggtta tatgcggatt ccagcgcgtt ccgttcgttt
     6301 cgggtgcttc cagttgatac cttcaagaag catggataac tgagccggag taaggtgcac
     6361 cttgccgtca cgggtgactg gccagacgaa gcggccccgc tccaggcgtt tggtgaagag
     6421 gcacagtccg tcactgtcag cccacaacac ttttatctgg tcaccccggc gtccgcggaa
     6481 gatgaacagg tgtccggaga acgggtcatc cttcaggacg ttctgaactt ttgatgccag
     6541 gccgttaaag ccatttcgca tatcggtgat acctgcaacc agccagatac gcgaacctgc
     6601 agggagagat atcatcagtg gctgctccct tttatttcgc ggataagtgt ctgtaataac
     6661 gccggcgtca gtttaccttt aagcctgaga gttccggccg gcagaaccag ctcacaacac
     6721 agactgtcgg acggtgtatt tatctgctct ggttcctgtg cgggggccgg gattttatta
     6781 tccggctccg gcgttaacgt cacgggaagc agtgccggca tattttttcc ggaaggcagc
     6841 aggccacctt tccggtattg atggcgccag ttgaagagca ggttatcgtt gattccgttt
     6901 tcccgggcga tctgcgccac acaggctccg ggctgtagtg actgctccac taaggcgatt
     6961 ttaaactcat aagggaagtt gggccgccgg ggacgttttt ttaccacggg ggcttcggat
     7021 ataacggtgc tttcaggacg tacgactggt accgtggaaa attgtccgta aaggcaggca
     7081 tcaagttcct gctccgacat gcctgcgggc aaaggccacg aaaggccagc tctccgaaag
     7141 cgcacgaaca tactacaaac tgttgatttt ggtacactca ggcgacgccc ggccacaacc
     7201 cggggtaaat gttcttcaaa gtgaagacgt aaagcttcag tgatccaggt ccggtgtttc
     7261 atacgatagt gtccattaaa aatgatggac attatttttg tagagccgga ggaaacagac
     7321 cagacggttt aaatgagccg gttaccttga tactcaggga gttatatctc cgaagagtat
     7381 ctcttatcat acgtcggttt actcccaatg tgatttgatg gtcaatgagt caggaataat
     7441 cccgtgaaaa aatgtgtgcc agtcctgctt tttagtgttc tttctgcttc ggttgccagt
     7501 catgcggaca gcaatgtttt tcaacaaaag gccgagaaga caaaaaacgg cattgttgcg
     7561 gatgttaatg ccagacagaa aatattcagt aacagactga agatacaact tcagatcgat
     7621 gagttgagag cagagctggc tggcaccaga gatgcccaaa aaagagagga tttgcgtaaa
     7681 aaactggagt ctctggagcg gcaaaagaaa tccttacgct gatgggggcg tattttcccc
     7741 ggtttcttcc ataacctttt taccgttcag atatttacgg gcgaggcgaa tgcgtcttgc
     7801 ctggtatacc gccgagtaag cctgggtggg attcacacca ttactgattt cactgaccac
     7861 atgacgctga tcagccagtg tcagcgtgta aacctgtttc tggatgaggt cacgggcttt
     7921 gtcatgctca tatccggcag caacaagggt gtcgagaagt cgtttgtctg ttttgttcag
     7981 tttatggcgg ggtgatttgc tcacatcgtt ctccgttttt tcatgatcca acagttttat
     8041 atctcatcct gatttgctcc ggtgttctgt ttcgacgttc tcaatgcctg cctccccata
     8101 gtgtgatcct tattgcaaaa atgaaaatcc atgctcttga catggacaat actgagtagt
     8161 aggttttact gtacataaaa ccagtggtta tatgtacagt atattgtttt taatttattg
     8221 ttttaattat caaagaggaa tttatggcta aagaactaag tggatatgga ccaactgctg
     8281 gtgagtctat ggggggaaca ggggcgaatc tgaatcagca aggtggaaat aataatagta
     8341 atagtggggt tcactggggt ggtggctctg ggcatggtaa taatggtggt cagggaaatt
     8401 caaattcatc tggttctaca tcaactgtga tgaaaaccgg tgagtcatat ctgactccgt
     8461 ggggtgatgt tgtcattaat aatgatggcc ttccggtgat gaatggtatt gttatgacag
     8521 aagagaattc aactctggtt gataatccat ttggaggggt ctccagagtt cttaattctt
     8581 taattagtga tatgccatca ctttttgcag aaagctcagg aaataacaac aataacactg
     8641 cttcagttaa tactgcacca acgaatgctc aggtcagtga tatggataag agttcgaagg
     8701 tggtgggtaa tgtaattaat gaaaaacaaa agcagaagaa taaaattgct acacagatta
     8761 gtgaaaaaca gaagaaaatc gaggagatga aaaaagtatt caagcatcat tcttatcatg
     8821 gtatcactga tcttgagagg gatgttgatg agttgcaaaa aaaatcaaat cagcttgatg
     8881 cagatattag caaacttaat tcttataaaa atacattgca aagtaaaatt ggtgatgtga
     8941 ataaacagaa ggaagctgaa gaaaaggctc gtgaaaatgc agaggtcgct gaacatgaaa
     9001 ctcttaatga agagaaacag gcggtggcgg aagcagagaa gcgtttagct gaggtgaaag
     9061 ctgaactggc gaaggctgaa agtgatgtac agagtaagca agctactgtt tccagagttg
     9121 caggagagct tgaaaacgct cagaagagtg ttgacgtgaa ggtcaccgga tttccgggat
     9181 ggcgtgacgt tcagaaaaaa ctacagcgtc agcttgaagc taagcaggct gagtactccg
     9241 cagttgaaaa tgaacttaag aatgctgtta gtttcaggga tggtaaggcc gctgaggtta
     9301 aagaggcaga gcagaaatta aaagaagctc aggatgctct tgaaaagagc cagataaaag
     9361 atgctgttga tactatggtt ggattttatc aatatataac cgaacaatat ggagaaaaat
     9421 acgcaaaaat tgctcaggat ttagcggaaa agtctaaagg aaagaaaatt caaggtgttg
     9481 atgaagctct ggcagcattt gaaaagtata agaatgtact ggataagaaa ttcagtaagg
     9541 ttgatcggga tgctattttt aatgcgttag agtctgttaa ttatgatgag ttatctaaga
     9601 atttaacgaa gatatcaaag tcgcttaaaa tcaccagtcg agtatctttt ctatatgatg
     9661 ttggttctga ttttaaaaat gcaatagaaa ctggt
//
