LOCUS       NZ_CP062859             8009 bp    DNA     linear   CON 16-JUN-2023
DEFINITION  Escherichia coli strain Res13-Lact-PEB14-36 plasmid unnamed.
ACCESSION   NZ_CP062859
VERSION     NZ_CP062859.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN16304205
            Assembly: GCF_015571815.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8009)
  AUTHORS   Poulin-Laprade,D., Brouard,J.-S., Gagnon,N., Turcotte,A.,
            Langlois,A., Matte,J.J., Carrillo,C.D., Zaheer,R., McAllister,T.,
            Topp,E. and Talbot,G.
  TITLE     Resistance determinants and their genetic context in bacteria from a
            longitudinal study of pigs reared under conventional and
            antibiotic-free husbandry practices
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8009)
  AUTHORS   Poulin-Laprade,D. and Brouard,J.-S.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-OCT-2020) Sciences and Technology, Agriculture and
            Agri-Food Canada, 2000 College, Sherbrooke, Quebec J1M 0C8, Canada
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 13-NOV-2018
            Assembly Method                   :: Unicycler v. 0.4.7
            Assembly Name                     :: QIA-13
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 1.00x
            Sequencing Technology :: Oxford Nanopore MinION and Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 06/16/2023 15:47:59
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,899
            CDSs (total)                      :: 4,779
            Genes (coding)                    :: 4,550
            CDSs (with protein)               :: 4,550
            Genes (RNA)                       :: 120
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 63 of 229
            Pseudo Genes (incomplete)         :: 152 of 229
            Pseudo Genes (internal stop)      :: 46 of 229
            Pseudo Genes (multiple problems)  :: 33 of 229
            Pseudo Genes (short protein)      :: 4 of 229
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP062859.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..8009
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="Res13-Lact-PEB14-36"
                     /serotype="O-untypeable:H11"
                     /isolation_source="feces"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:562"
                     /plasmid="unnamed"
                     /geo_loc_name="Canada: Quebec"
                     /collection_date="2017-03-13"
                     /collected_by="Agriculture and Agri-Food Canada"
     gene            328..513
                     /locus_tag="IMP96_RS00005"
                     /old_locus_tag="IMP96_00005"
     CDS             328..513
                     /locus_tag="IMP96_RS00005"
                     /old_locus_tag="IMP96_00005"
                     /inference="COORDINATES: protein motif:HMM:NF024139.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_112045314.1"
                     /translation="MKPKLLRVDQVLELLNISRGKLYRLLKNEPLFPRPIKLGGMLYFI
                     PEEIDEFINLQRRVER"
                     /name="helix-turn-helix domain-containing protein"
     gene            514..1101
                     /locus_tag="IMP96_RS00010"
                     /old_locus_tag="IMP96_00010"
     CDS             514..1101
                     /locus_tag="IMP96_RS00010"
                     /old_locus_tag="IMP96_00010"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_195714392.1"
                     /translation="MSNLNDIKSIDSVTRITFIEGTINTALLYEAAPVNLTVRVPVNFL
                     ERRHDTKEDGFIRAIMFMAAAGPLKCGGGKNESDLNYVMGVYYGRNIKTDIERSIGISL
                     IQEYRELPGFGRKYHYAFRSKEDVRSALSGVPSEYISKAEKRACFKLYPSNKQLGIAPP
                     PQMGFQFNACRLTSAVMRRLNKRDRLLYRCRH"
                     /name="hypothetical protein"
     gene            1190..1483
                     /locus_tag="IMP96_RS00015"
                     /old_locus_tag="IMP96_00015"
     CDS             1190..1483
                     /locus_tag="IMP96_RS00015"
                     /old_locus_tag="IMP96_00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001685281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_195714401.1"
                     /translation="MSFIKNHFDGRDLPLIEAQKQFKKEIDLTVEEIINELPEMLRPII
                     AERAEELVENAPDALKKPDSVTGDVMASAKAVYMRLLFSVSNQLGHGANWLK"
                     /name="hypothetical protein"
     gene            1505..1750
                     /locus_tag="IMP96_RS00020"
                     /old_locus_tag="IMP96_00020"
     CDS             1505..1750
                     /locus_tag="IMP96_RS00020"
                     /old_locus_tag="IMP96_00020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001410737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_195714393.1"
                     /translation="MTFDELEAKGAELVEKRNKATGSERELLNKEIASLRQAQLKALAE
                     EITAALKLAIPEMVSIAIKSSKVNKAIEDLRGEING"
                     /name="hypothetical protein"
     gene            1743..2171
                     /locus_tag="IMP96_RS00025"
                     /old_locus_tag="IMP96_00025"
     CDS             1743..2171
                     /locus_tag="IMP96_RS00025"
                     /old_locus_tag="IMP96_00025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001685282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_195714394.1"
                     /translation="MDEKIKALLNQVRILEEEANKHSAGNERAELFRAAGRIKNQVATL
                     LNQCGKSEPGSKVPDPNAYFSAQLPEGFENYFHGDYLTPEMESYFNNLTPEMESYFSVP
                     KIQNFDNAPSAKPEPAKTQEFDDWELRRRILNAIGIPC"
                     /name="hypothetical protein"
     gene            2231..2728
                     /locus_tag="IMP96_RS00030"
                     /old_locus_tag="IMP96_00030"
     CDS             2231..2728
                     /locus_tag="IMP96_RS00030"
                     /old_locus_tag="IMP96_00030"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_251122126.1"
                     /translation="MNKIEESRIPGIAQGEIVKGIISRGERITNMTPEERAAAREKARE
                     IQNQRHAEWRANADKLKTDFSDKGYWHRAARHYGVTMPGHHVPGTYIPTIRKAMKKMGL
                     LKPGEGFTKAFRETIDEDYRTFVANNPDWPAFAIIGLLLECALERDGIDHLPADRRQIV
                     IR"
                     /name="hypothetical protein"
     gene            2740..3018
                     /locus_tag="IMP96_RS00035"
                     /old_locus_tag="IMP96_00035"
     CDS             2740..3018
                     /locus_tag="IMP96_RS00035"
                     /old_locus_tag="IMP96_00035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000880419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_195714395.1"
                     /translation="MLCLKMLHNMNRAISIRVAELKAAPTAQQPNKRASLRCFAELVLN
                     TIAGLLRSKEGNTMSPDEKVFYRRCQLVARDIHAQLESVSADIDADI"
                     /name="hypothetical protein"
     gene            3029..3280
                     /locus_tag="IMP96_RS00040"
                     /old_locus_tag="IMP96_00040"
     CDS             3029..3280
                     /locus_tag="IMP96_RS00040"
                     /old_locus_tag="IMP96_00040"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_195714396.1"
                     /translation="MADRGRPTLYSKELEKEICIHALEGMSMRQIAKQPGMPSFRTLLR
                     WTKRYPDFRQTLRECLWLGYHDAVIDRFNQQHPDLVGY"
                     /name="hypothetical protein"
     gene            3988..4614
                     /locus_tag="IMP96_RS00045"
                     /old_locus_tag="IMP96_00045"
     CDS             3988..4614
                     /locus_tag="IMP96_RS00045"
                     /old_locus_tag="IMP96_00045"
                     /inference="COORDINATES: protein motif:HMM:NF012464.2"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0000150 - DNA strand exchange activity [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="WP_195714397.1"
                     /translation="MANAAYIRVSTIAQDTSRQREAIANTGLKFEKIVEEKRSGKDLDR
                     PALRELCGWLREGDDLYVASIDRLGRKLTDVMNVVDELRQKGVRVHFLKEGQIIGGEMN
                     AVQEFFIQLMAAAAQMERSFMLERQKEANEARKAAGKPIGRGLAPKIKQQLPYILKDME
                     QRKESMSDLCRKYKVARSTMYRIRDNAIRDGKLPEIVKVKKVVIN"
                     /name="recombinase family protein"
     gene            4841..5536
                     /locus_tag="IMP96_RS24320"
     CDS             4841..5536
                     /locus_tag="IMP96_RS24320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001521010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="WP_251122127.1"
                     /translation="MEYMDWVDLGMKKRDRKMTKADVGKAMGFSGNGGINHKFNRSRDT
                     TNANADDVLKMLAVVGWQAIIFDSNGKIVMGTAHVDSPEGMEQKFIKRCEELSKENEAL
                     KAEIAQLKAKAVPTVDGTDVTADVTADVTADVTADVTPRVRRNPKPQLFTIADLKLALK
                     AKGGVFEGSLNQLGDLLAVEWCNIDSRADETLKPSKVVVCRLLKAAVEAGVVQVDADKP
                     QGPKTIVLL"
                     /name="XRE family transcriptional regulator"
     gene            5746..6333
                     /locus_tag="IMP96_RS00055"
                     /old_locus_tag="IMP96_00055"
     CDS             5746..6333
                     /locus_tag="IMP96_RS00055"
                     /old_locus_tag="IMP96_00055"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_195714398.1"
                     /translation="MIKKSNVALVENSRIAVKATNFNKQDAIPDGWQKFGNVPPDAVIT
                     MLGTVKEFTYDRKVWRKTPDGTGYMWHPANHQQEQAQNDAGTQNIYCSYVCLGAGYKAF
                     KRAFAAYEGKDKTSIWATLSIMAANARNDNGAWIVKVSQKAIGDELNKDRKAIGRYIKQ
                     LVGAGLIFPIGRYIPGQDGRQYRICYQVEDAK"
                     /name="hypothetical protein"
     gene            6330..6653
                     /locus_tag="IMP96_RS00060"
                     /old_locus_tag="IMP96_00060"
     CDS             6330..6653
                     /locus_tag="IMP96_RS00060"
                     /old_locus_tag="IMP96_00060"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_195714399.1"
                     /translation="MSAVISLSEVRRRKETAKEAEIASNWMWVLMLPLSDRYIEVGEDT
                     YWLYVGDNDGSDKSDWIWTRHSELSRDEFLWLINRGVGYFLPPDGCEMEVCYNVEWFSF
                     SRV"
                     /name="hypothetical protein"
ORIGIN
        1 gaaacgtacc actttcaaaa attagcgcgt tgtaggtacc attcctttcg aatatgggcc
       61 attcaggata gtgatctgtg gttggtcggc aggagacgga ttgggtatgg ccctgattag
      121 ccactaagga tgagaatcgt tatcagctct ggataagcgg ataaacgggc gcgcgattat
      181 aagccaactt tgtacggatg taaacaagct gatacaatat tagtcagatg gtgggaattg
      241 tgcctatcat cgggagaaca gattaatact tcttggcctt aatcccgctt tccgtattgg
      301 ttagcgggtt tttttgttgg aggcaatatg aagccgaaat tattgagagt agatcaggtt
      361 cttgagctgc ttaatatcag ccgtgggaag ctatacagac ttctaaaaaa tgaaccatta
      421 ttcccgcgcc ccattaaact tggcggcatg ctttatttca taccagaaga aatcgacgag
      481 tttattaatc tacagcggcg ggtggaaaga taaatgagca acttaaacga catcaagtct
      541 attgactctg tgacgcgcat tacctttatt gagggaacga taaatactgc gttattgtat
      601 gaagccgctc cggttaattt aactgtacgg gtgcccgtta attttcttga acgtcgccac
      661 gacactaagg aagatggatt tattcgcgca ataatgttta tggcagcagc tggcccgctg
      721 aaatgtggtg gcggtaaaaa cgaatccgat cttaattacg tcatgggcgt ttattacggt
      781 cgcaacatta agacagatat tgagcggtcg ataggtattt ctttaatcca ggaatatcgc
      841 gagctgccag gttttggacg caagtaccat tatgctttcc gtagcaaaga agatgttcgc
      901 tcagcactgt caggcgtgcc gtctgaatat atcagcaagg ccgagaagcg cgcttgcttt
      961 aagctgtacc caagcaacaa gcagttaggc atcgcgccgc caccgcagat gggttttcag
     1021 ttcaacgcct gccgtctgac cagcgccgtg atgcgcagat taaataaacg cgatcgcctt
     1081 ctctaccgct gccgacatta aaaaataggg gggcaactac caaccagaaa ttctattttt
     1141 cgcgtagcaa caccattcta tagaaagcta attaattgga gataagaaaa tgtcctttat
     1201 taaaaatcac tttgatggcc gcgacttacc gcttattgaa gcacagaaac agtttaaaaa
     1261 agagattgat ttgaccgttg aagaaattat taacgagctg cctgaaatgc tccgcccgat
     1321 tatcgctgag cgggcagaag aacttgtcga aaacgcgccg gacgcactta aaaaaccaga
     1381 ttctgtaacg ggagacgtaa tggcgagtgc gaaagccgta tatatgagat tgttattttc
     1441 tgttagcaac caattgggtc atggtgcgaa ttggcttaaa taactatgaa cgggagacat
     1501 aattatgaca ttcgacgaac tagaagcaaa aggcgctgaa ttagttgaga agcgtaataa
     1561 agcaaccggg agcgaacgtg agctactaaa taaggagatc gcaagtcttc gccaggcaca
     1621 attaaaagcg ctggcagaag aaatcacggc ggctttaaaa ctggctattc cggaaatggt
     1681 tagcatcgcc atcaagtcca gcaaggtaaa taaagcgatc gaagatctga gaggtgaaat
     1741 caatggatga gaaaatcaaa gcgctattaa atcaggtgcg gatccttgaa gaagaagcaa
     1801 ataaacattc tgctggcaat gaacgcgcag aattgtttag agctgccgga cgtattaaga
     1861 accaggtggc aacgctatta aatcagtgtg gtaaaagcga accgggaagt aaagtaccag
     1921 atccgaacgc gtatttctcc gcacagctgc cggaaggctt cgaaaattac ttccatggcg
     1981 actacctgac tccggaaatg gaatcatatt tcaacaacct tacgccggaa atggagtcat
     2041 atttttccgt gccgaagatc cagaacttcg acaacgcgcc ttccgctaaa ccggagccag
     2101 caaaaaccca ggaattcgat gactgggaac tgcgccgccg gatactcaac gcgattggca
     2161 tcccgtgcta atggcagaaa gaagccccgt cttgcgcggg gcaaacaatg cgaaacgaat
     2221 ggtgatcaag atgaacaaaa tcgaagaaag cagaatccct ggaatcgccc aaggcgaaat
     2281 tgttaagggg attatatcgc gcggagaacg catcacaaac atgacgcctg aggaacgcgc
     2341 cgccgccagg gaaaaggcca gagagataca aaaccagcgg cacgctgaat ggcgggctaa
     2401 tgccgacaag ctgaaaaccg acttttccga caaaggatac tggcaccgcg cagccagaca
     2461 ctacggtgta accatgccag ggcatcacgt acctggcaca tacatcccga cgatccgaaa
     2521 agcgatgaag aagatgggtc tgttaaaacc aggagaaggc tttacgaagg cattcagaga
     2581 aaccattgat gaagactacc ggacatttgt tgccaacaac cctgattggc ctgcgttcgc
     2641 aattattggt ctgctactgg agtgcgcgct ggaaagagat gggattgacc atcttcctgc
     2701 tgaccgccgc cagatagtca ttcgctaagg agaaaaacca tgctttgcct caagatgctt
     2761 cacaacatga accgagccat ttctatccgc gtagccgaac tgaaagccgc gccaacagcc
     2821 caacagccga acaagcgcgc cagccttcgg tgctttgccg aactggtatt gaatacgatc
     2881 gcaggtctgc tcaggagcaa ggaagggaat actatgtctc ctgatgagaa agtgttctat
     2941 cgccgctgcc agttggttgc cagggatatc catgctcagc tggaaagcgt atccgcagac
     3001 atagacgcag acatctgaag gaggaatcat ggcagatcgc ggaagaccta ccctgtactc
     3061 aaaggagctg gagaaagaaa tttgtattca tgcgctggaa ggaatgagta tgcgccagat
     3121 agcgaagcag ccaggaatgc cttcgttccg tactctgttg cgctggacga aacgctatcc
     3181 ggatttcagg cagacgctgc gcgagtgtct ttggcttgga taccacgatg cggtcataga
     3241 cagatttaat cagcagcatc cagacctggt cggctactaa tttccggaca agtacacgcg
     3301 cgtagccaaa tcatcaacgc aactaatatt tcccttctat attgccgcta caattaacgc
     3361 attttggggc cgtcttttgc gttcaaatgg cctcaaaagt gccttagcga ccccttagcg
     3421 accacttagt gcggtgttaa acgggtattt tccaccatta aattcgcgaa caactctgtt
     3481 caaaaccgcg ccagctgggc ttttgctcta tatggccccc tatttatggc accaaatgag
     3541 ataagacacc gccaccttta gccaaaaatc aatcagcccg ccttattaat cctgccattg
     3601 tttcaatatt gcttctcttc ttccggatag ttctaccgcc gacgccattg gcgcacaccg
     3661 gccaggcggc ctttcttttg ttccagacca gaaatttctg gtcaacccaa gtcattgatt
     3721 ctttcacgaa cctgcgccgc aggccactgg accatcccgc cacaagcgcc ctactcgaaa
     3781 aatccctcac aactacccac aggtttttct atttttcacg tacacgcgcc tgaagcaccc
     3841 ttcccacatc cttgatattt cgcacaccct gccaaaagcg tgatctgcga caaaatttgt
     3901 actttttgcc gttgccaggt ggtctatatg ggaataatct cacaatcaaa gagaggtatt
     3961 ggtatgtggg aaatggagga aagtatcatg gctaacgcag cttatattcg tgtttctact
     4021 atcgctcagg atacatcgcg ccaacgcgag gctatcgcta acactggcct gaagtttgag
     4081 aaaatcgttg aagagaaaag gtcaggtaaa gacctcgatc gcccggcact gcgagagcta
     4141 tgcggctggt tacgtgaagg tgacgacctg tacgtcgcat ctattgatag actggggcgc
     4201 aaactgactg acgtgatgaa tgtggttgat gaactgcggc agaaaggggt tcgggtccac
     4261 tttctgaaag aagggcagat catcggtggc gagatgaacg cggttcagga gttcttcatc
     4321 cagctgatgg cagcagccgc gcagatggag cgcagtttta tgctggagcg ccagaaagag
     4381 gcaaacgagg cgcggaaggc ggcagggaaa ccgattgggc gtgggctggc accgaagatt
     4441 aagcagcagc tgccgtacat cctcaaggac atggaacaac gcaaggagtc catgagcgac
     4501 ctttgccgca agtacaaagt ggcacgaagc acaatgtacc ggatccgcga caacgccatt
     4561 cgcgatggta agctgccgga aattgtgaag gtcaaaaagg tcgtcatcaa ctaaccaacc
     4621 accacaaagg ggccagcaac cacgccagcc cctttaatct ccctcacaat ccccgctgac
     4681 attatagcaa accgttgtat cgaagctatt ctcgcgatgt aacagcgccg ggttttttgt
     4741 gatctgtgtc aacatgccag cgtttgccag taccgtgaat tagtgcgaag tggtaccgtt
     4801 gtatcgaagg caataactcg atgtaacggt ggggtgattt atggaatata tggattgggt
     4861 cgatctgggc atgaagaaac gcgatcgcaa gatgactaag gcagacgtcg gaaaggcaat
     4921 gggcttcagc ggtaacggag gcatcaatca caagtttaat cgcagccgcg atacaacaaa
     4981 cgcgaacgct gatgatgtgc taaaaatgct cgcagtagtc ggctggcaag ccatcatatt
     5041 cgacagcaac gggaagatcg tcatggggac agcgcacgta gatagccctg aaggaatgga
     5101 acagaaattc atcaagcgct gtgaagaatt gagcaaggag aatgaggcac tgaaggctga
     5161 gattgcgcag ctgaaggcaa aagccgttcc aacggttgat ggcactgatg taacagccga
     5221 tgtaacagcc gatgtaacag ccgatgtaac agccgatgta acgcctcgag tgcgcaggaa
     5281 tccgaagcca caactattta ccatcgctga cctgaagctg gcactgaagg ccaaaggcgg
     5341 cgtttttgaa ggtagcctga accagcttgg cgatctgtta gccgttgagt ggtgtaacat
     5401 cgacagccgc gctgatgaaa cactgaaacc gagcaaagtc gttgtttgcc gtttgctaaa
     5461 agcagccgta gaagctggag tggttcaggt tgacgctgac aagccgcaag ggccaaagac
     5521 aattgtgctg ttgtaacggc gtgcgcgctg atgtaacggt cgatgtaacg gtcgatgtaa
     5581 cggtagcatc atttcataaa acgcaacgat acgcgtgatt gttgcgaaaa gtgaaataat
     5641 ccagtcagaa caacagtttg cgagagtgtt ttaatggcag tatcgtagaa agcataaagc
     5701 ccgggggcgt ccgggctatc aatcaacaaa caggagaacc agatcatgat taagaaatcg
     5761 aacgtggcgc ttgttgagaa ctcaagaata gcagtcaaag caacaaattt caacaaacaa
     5821 gacgctatac ctgacggctg gcaaaagttc ggaaacgtac cgccagatgc tgttattacc
     5881 atgcttggaa cggtaaaaga gtttacatat gacagaaaag tatggcgtaa gacaccggac
     5941 ggcactggtt acatgtggca cccagcaaat caccaacaag agcaagcgca gaacgacgca
     6001 ggaacgcaaa acatatactg ctcttacgtt tgccttggtg ctggttataa agcctttaaa
     6061 cgagcatttg ctgcgtatga gggcaaagat aaaacgtcaa tatgggctac gttgagcatc
     6121 atggcagcaa acgcacgcaa tgataacggc gcgtggatcg tcaaagtcag ccagaaggca
     6181 attggcgatg aattaaacaa agaccgcaaa gctataggga gatatatcaa gcagcttgtt
     6241 ggcgcgggat taatcttccc tatcggcaga tatatccctg ggcaggatgg cagacagtat
     6301 cgaatttgct atcaggtgga ggacgcgaaa tgagtgcggt aattagttta agcgaagtac
     6361 gcaggcgcaa agagacggca aaggaggccg agattgccag caactggatg tgggtgctga
     6421 tgctccctct ttccgacagg tatatcgaag tgggcgaaga cacctactgg ctctatgttg
     6481 gcgacaatga tggcagcgac aagagcgatt ggatctggac gagacacagc gaattgtcca
     6541 gggatgagtt tttgtggcta atcaatcgtg gcgttggcta tttccttccg ccggacgggt
     6601 gcgaaatgga agtttgctac aacgtcgagt ggttcagttt ctcaagagtc tgaacacaaa
     6661 aacacagacg cggggcggct tcggtcgcct tttttgttga cttatccaca atgtcccacc
     6721 acgcctcacc cccttaattt ccatgtccca ccacacacca ccccatgtcc caccacacac
     6781 caccccatgt cccaccacac accaccccat gtcccaccac acaccacccc atgtcccacc
     6841 agacacccac attatatgag ttatatgatt ttataagatc agtaagagtg taagaagcgc
     6901 gtgcgcgcgc gagggattta ctgttttgtg gataacttcc aaatcaacat cgtgcccatt
     6961 tgctggaaag tatcttcaac atcctgcctg acgaatgaga tcgagatagc tgatagttcc
     7021 cattggctta gcaggaggga gacggcgctt cgcttgcccc cacgtccctt gccaggcggg
     7081 tatctctgtt cggcccggtc tgccctacaa cctgtagaca cctaacctcc gtgcggcagc
     7141 cttttatcgc ttcgctcaat aaaatcggta gaccgttcgg tttagacaaa aagtgctgaa
     7201 cgtattacgc atcacaaaaa atggctgtaa ggtatttaca gatgccaatt tttctgaatg
     7261 gaaaacggct agatattaac cagtgtcgaa gcccacactt aaaatttggc gagtacgtgt
     7321 cgggccagca cgaaaacatg gcgtcaccca gcaaatcaga gagtccactg ggctggttaa
     7381 aaaaatctaa cggacggtaa cagtaaccgg gcggaagtaa tgggatccga acgcagccgc
     7441 gccagaggct ggttgaaacg ggtttatgcg gttgccccca ccgtagtgaa gggattgggg
     7501 cgaggccggt cagggcgctg gctacaggga cagaaacaga aagccacccg atattcccgg
     7561 cgaacttact taacaggcaa tgacttctcc gcaccccatt caaagaccgg gatctgccca
     7621 ttaatcccta ccagataata gtcccaacaa attttgcgtt ttcctagata cccttccccg
     7681 ccaatttttc gaattgagac atacaaaaca gcccgcaagg cctgattcta ccgtcgggat
     7741 ttgtctgccc cattcagcat gcttgtggga ataggtccga tcgtcgtttc tactgcggct
     7801 cctgaggtct gaaacgcgct gaagccatcc agggtataca aacctatgcc caaaaatttt
     7861 gttaatcctg aggccatttg tctgctgacc agacgcgtat ccgttaatgc gaaatcatta
     7921 aatttttcgc attgccagaa actgagtgaa aatggatgcc cctgaggcgc gctcattcgc
     7981 attctgacga gctaatctcc agacctatg
//
