LOCUS       NZ_CP062459            13393 bp    DNA     circular CON 28-MAY-2024
DEFINITION  Staphylococcus aureus strain NAS_OP_153 plasmid unnamed1, complete
            sequence.
ACCESSION   NZ_CP062459
VERSION     NZ_CP062459.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN15567997
KEYWORDS    RefSeq.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 13393)
  AUTHORS   Cella,E., Sutcliffe,C., Jubair,M., Weatherholtz,R.C., Reid,R.,
            Reid,A., Quay,D., Azarian,T.H. and Hammitt,L.
  TITLE     Carriage prevalence and genomic epidemiology of Staphylococcus
            aureus among Native Americans in the southwest US
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 13393)
  AUTHORS   Cella,E., Sutcliffe,C., Jubair,M., Weatherholtz,R.C., Reid,R.,
            Reid,A., Quay,D., Azarian,T.H. and Hammitt,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JUL-2020) Burnett School of Biomedical Sciences,
            University of central Florida, 4111 Libra Dr, Orlando, FL 32816, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 2020
            Assembly Method                   :: Uniclycler v. 0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 50x
            Sequencing Technology             :: Illumina MiSeq; Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_022699775.1-RS_2024_05_28
            Annotation Date                   :: 05/28/2024 03:23:59
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 17
            CDSs (total)                      :: 17
            Genes (coding)                    :: 16
            CDSs (with protein)               :: 16
            Genes (RNA)                       :: 0
            tRNAs                             :: 0
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP062459.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..13393
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /strain="NAS_OP_153"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /plasmid="unnamed1"
                     /collected_by="UCF"
     gene            join(13098..13393,1..1462)
                     /gene="blaR1"
                     /locus_tag="IF780_RS00005"
                     /old_locus_tag="IF780_13770"
     CDS             join(13098..13393,1..1462)
                     /gene="blaR1"
                     /locus_tag="IF780_RS00005"
                     /old_locus_tag="IF780_13770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactam sensor/signal transducer BlaR1"
                     /protein_id="WP_001096381.1"
                     /translation="MAKLLIMSVVSFCFIFLLLVFFRYILKRYFNYSLNYKVWYLTMLA
                     GLIPFIPIKFSFIKFNNVNNQAPTVESKSHDLNHNINTTKPIQEFTTDIHKFNWDSIDN
                     ICTVIWIVLVIILSFKFLKALLYLKYLKKQSLYLNENEKNKIDTILFNHQYKKNIVIRK
                     AEAIQSPITFWYGKYIILIPSSYFKSVIDKRLKYIILHEYAHAKNRDTLHLIIFNIFSI
                     IMSYNPLVHIVKRKIIHDNEVEADRFVLNNINKNEFKTYAESIMDSVLKTPFSNKNILS
                     HSFNGKKSLLKSRLINIKEADLKKQSKLILIFICIFTFFIMIIQSQFLMGQSLTDYNYK
                     KPLQSDYQILDESKNFGSNSGSFVMYSMKKDKYYIYNEKESRKRYSPDSTYKIYLALFG
                     LDRHIISDKNSRMSWNHKHYLFESWNKEQDLNTAMQNSVNWYFERISNQIPKNYTAAQL
                     KQLNYGNENLGSYKSYWMEDSLKISNLEQVIVFKNMMEQNNHFSKKAKNQLSSSLLIKK
                     NEKYELYGKTGTGIVNGKYNNGWFVGYVITNHDKYYFATHLSDGNPSGKNAELISEKIL
                     KGMGVLNDQ"
                     /name="blaR1"
     gene            1452..1832
                     /gene="blaI"
                     /locus_tag="IF780_RS00010"
                     /old_locus_tag="IF780_13775"
     ARG             1453..1829
                     /name="blaI"
                     /gene="blaI"
                     /gene_name="penicillinase repressor BlaI"
                     /tool="amrfinderplus"
                     /drug_class="BETA-LACTAM"
                     /identity="98.41"
                     /coverage="100.0"
     gene            2096..2674
                     /locus_tag="IF780_RS00015"
                     /old_locus_tag="IF780_13780"
     CDS             2096..2674
                     /locus_tag="IF780_RS00015"
                     /old_locus_tag="IF780_13780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000690624.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0000150 - DNA strand exchange activity [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="WP_000690637.1"
                     /translation="MKIGYARVSTGLQNLNLQEDRLNTYGCEKIFNDHMSGSKSKRPGL
                     DKAIEFARSGDTIVVWRLDRLGRNMEDLITLVNELNERGVSFHSLEENITMDKSSSTGQ
                     LLFHLFAAFAEFERNLILERSSAGRIAARARGRYGGRPEKLNKQDLNLLKTLYDNGTPI
                     KTIAEQWQVSRTTIYRYLNKLEAKENKNK"
                     /name="recombinase family protein"
     gene            complement(3035..3778)
                     /locus_tag="IF780_RS00020"
                     /old_locus_tag="IF780_13785"
     CDS             complement(3035..3778)
                     /locus_tag="IF780_RS00020"
                     /old_locus_tag="IF780_13785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000843735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="WP_000843732.1"
                     /translation="MKSVKKLSEELGVSKQTIFNNIKRLNIETIKQENTSFIKEDTDIE
                     KIIQRVNENKKKYGFESTTEDKQKKGSDNINFESKSDAQIVEILKNQIDTLNNQIEKQE
                     SRHETTIEFYRKELQERSKLLENQQVLALESNKKIQKLENQLEKEKHLNYPFDTSTNTR
                     QNVDAQEKTYTTNPVNINRDQEETKETEIQYKDISGSQSDESTQGEEAKKEDVPANLND
                     NDSDIEEKSEETEPKKGFWSRLFGS"
                     /name="DUF536 domain-containing protein"
     gene            4204..5187
                     /locus_tag="IF780_RS00025"
                     /old_locus_tag="IF780_13790"
     CDS             4204..5187
                     /locus_tag="IF780_RS00025"
                     /old_locus_tag="IF780_13790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001181101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="WP_001181107.1"
                     /translation="MQNQYFTVQENYKERFYQIPKVFFTSENYKNLTNDMKIAYAILRD
                     RLNLSIKNSWVDEDGNIYFVYSNEKLMEILNCKKEKLTKIKKGLENDGLLIQKRRGLNK
                     PNILYLMKPIVTERDIYKIEKEENDVEPYGEKEVRKSNVQKFENRTSRSSKIERPEVRK
                     SNTNDTDFNDIDFNDTENNDMNDLNDIKYKNEISNHSNHSNQFTHNFDDKEMLLQEFPE
                     QLTNYLLKYDYRDLEIIKAVILKAKKSFNSRHEDMHYMLEDIEDEILTSLKRLKKAIHD
                     RGVKGQKETIKSMQAYLMQTILTELEETHALYMRRKNMKQYNIFNQ"
                     /name="replication initiator protein A"
     gene            <5207..5497
                     /locus_tag="IF780_RS00030"
                     /old_locus_tag="IF780_13795"
                     /pseudo=""
     CDS             <5207..5497
                     /locus_tag="IF780_RS00030"
                     /old_locus_tag="IF780_13795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009384749.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006260 - DNA replication [Evidence IEA]"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /name="protein rep"
     gene            6080..6268
                     /locus_tag="IF780_RS00035"
                     /old_locus_tag="IF780_13800"
     CDS             6080..6268
                     /locus_tag="IF780_RS00035"
                     /old_locus_tag="IF780_13800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_242643495.1"
                     /translation="MNKNTVLDEGYVIETILNVFFLLGLIFISHLENLYILIPYVILMG
                     INAIYLVVKVMNFKKNN"
                     /name="hypothetical protein"
     gene            6302..6424
                     /locus_tag="IF780_RS00085"
     CDS             6302..6424
                     /locus_tag="IF780_RS00085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000491229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000491229.1"
                     /translation="MFSHCFFIPIGVVAYFLFFYKDDAFDEMEEKYDYHEDNKK"
                     /name="hypothetical protein"
     gene            complement(6589..7128)
                     /locus_tag="IF780_RS00040"
                     /old_locus_tag="IF780_13805"
     CDS             complement(6589..7128)
                     /locus_tag="IF780_RS00040"
                     /old_locus_tag="IF780_13805"
                     /inference="COORDINATES: protein motif:HMM:TIGR04450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative immunity/bacteriocin fusion bifunctional
                     protein"
                     /protein_id="WP_001817824.1"
                     /translation="MTKKEISFLEQLQNKNEDMQKFEKQMKKEGFKKIKLEDKNSKLKY
                     LATEANDKNAYGYIANNAYKNEKTKEIVVSETIYDNYNKKITKFIAEKRSMTENKEGKI
                     LINKNYVEYKAEKVDKNRPVTYAGFKWNGKAFACSMAGLYACAHYCGVWAIVNPIAGGV
                     CEAVCGTAFAAGCSVG"
                     /name="putative immunity/bacteriocin fusion bifunctional
                     protein"
     gene            complement(7085..7282)
                     /locus_tag="IF780_RS00045"
     CDS             complement(7085..7282)
                     /locus_tag="IF780_RS00045"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_242643496.1"
                     /translation="MKIIKLFSIISLFLLVFSANFSNAYANEEQKSSLLENQKEKIEKQ
                     KHRSITNDQERNKFSRTIAK"
                     /name="hypothetical protein"
     gene            7392..7586
                     /locus_tag="IF780_RS00050"
                     /old_locus_tag="IF780_13810"
     CDS             7392..7586
                     /locus_tag="IF780_RS00050"
                     /old_locus_tag="IF780_13810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001004925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001004925.1"
                     /translation="MNENLEKYIKSLPLLGLLISILLIVLFFFILDVDEDLFVVFLYCL
                     LPLIVNLSIYGFYVFVKRK"
                     /name="hypothetical protein"
     gene            8702..9319
                     /gene="cadD"
                     /locus_tag="IF780_RS00055"
                     /old_locus_tag="IF780_13815"
     ARG             8703..9316
                     /name="cadD"
                     /gene="cadD"
                     /gene_name="cadmium resistance transporter CadD"
                     /tool="amrfinderplus"
                     /drug_class="CADMIUM"
                     /identity="100.0"
                     /coverage="100.0"
     gene            9338..9685
                     /locus_tag="IF780_RS00060"
                     /old_locus_tag="IF780_13820"
     CDS             9338..9685
                     /locus_tag="IF780_RS00060"
                     /old_locus_tag="IF780_13820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020366422.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0003700 - DNA-binding transcription factor activity
                     [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="WP_000119690.1"
                     /translation="MSYENTCDVICVHEDKVNNALSFLEDDKSKKLLNILEKICDEKKL
                     KIILSLIKEDELCVCDISLILKMSVASTSHHLRLLYKNEVLDFYKDGKMAYYFIKDDEI
                     REFFSKNHEGF"
                     /name="metalloregulator ArsR/SmtB family transcription
                     factor"
     gene            9842..9934
                     /locus_tag="IF780_RS00065"
                     /old_locus_tag="IF780_13825"
     CDS             9842..9934
                     /locus_tag="IF780_RS00065"
                     /old_locus_tag="IF780_13825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002495832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="WP_001791613.1"
                     /translation="MLIIFVHIIAPVISGCAVAYYTYWLSKRNK"
                     /name="type I toxin-antitoxin system Fst family toxin"
     gene            complement(10106..11077)
                     /locus_tag="IF780_RS00070"
                     /old_locus_tag="IF780_13830"
     CDS             complement(10106..11077)
                     /locus_tag="IF780_RS00070"
                     /old_locus_tag="IF780_13830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000481285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="WP_000481279.1"
                     /translation="MFNAVVVRKSASNVSYKVEKVDESHLSKGDVLVKVVYSSINYKDM
                     LALQYKGGVIRDYPMIPGIDFAGIVESSSNDKFKEGDNVLVTGYDVGVTHTGGFSEYAQ
                     VPGEWIVPIPQEMSIKESMVYGTAGFTAALSIFELEKYGMNIKSQPEILVSGATGGVGS
                     VAIQILHKIGYENITAIVRKEKQIEIAKKLGAKQVIIIEDDNKPLKKGIFDYFLDTVGS
                     NVTLYGLKRLNYQGVATVCGNVAGNSLNANILPFILRGIKLIGIDSVYVDHNIREDIWS
                     KLANEWNIGNSLLYNEIGFEEFYKTIDQLLKGQHLGRTILKV"
                     /name="oxidoreductase"
     gene            11201..11620
                     /locus_tag="IF780_RS00075"
                     /old_locus_tag="IF780_13835"
     CDS             11201..11620
                     /locus_tag="IF780_RS00075"
                     /old_locus_tag="IF780_13835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000418034.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0003700 - DNA-binding transcription factor activity
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="WP_000418035.1"
                     /translation="MELDNCINYLLSTSQNRVFKHFKSLLSPYNITPAEYGVLNCLINS
                     NLNTPKAIGNTLNLEPSTISGILDKMSKKDLITRQTDNENRRTVLINSTNKAKDLWPTL
                     EKKAYQLNNHYFANLTNEETVVFKKILLKINKTTF"
                     /name="MarR family transcriptional regulator"
     gene            complement(12146..12991)
                     /gene="blaZ"
                     /locus_tag="IF780_RS00080"
                     /old_locus_tag="IF780_13840"
     ARG             complement(12150..12991)
                     /name="blaZ"
                     /gene="blaZ"
                     /gene_name="penicillin-hydrolyzing class A beta-lactamase
                     BlaZ"
                     /tool="amrfinderplus"
                     /drug_class="BETA-LACTAM"
                     /identity="100.0"
                     /coverage="100.0"
     ARG             complement(12147..12991)
                     /name="PC1 beta-lactamase (blaZ)"
                     /gene="PC1 beta-lactamase (blaZ)"
                     /gene_name="BlaZ beta-lactamase"
                     /tool="rgi"
                     /drug_class="penam"
                     /identity="96.8"
                     /coverage="100.0"
     Typing          5568..5732
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="2.42e-81"
     Typing          complement(3036..3778)
                     /name="rep_cluster_1733"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          4205..5187
                     /name="rep_cluster_2100"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="99.593"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          5319..5489
                     /name="rep_cluster_1017"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.186"
                     /coverage="91.0"
                     /evalue="6.2e-68"
ORIGIN
        1 ggattcaatt gataatatct gcacagttat ttggatagtt ttagttatta ttttaagttt
       61 taaatttttg aaagccttat tatatcttaa atatttaaag aaacagtcac tttatctaaa
      121 cgaaaatgaa aaaaataaaa tagatacgat acttttcaac catcaatata aaaaaaatat
      181 tgtgattcga aaagcagagg ctattcaatc tccaataact ttttggtatg ggaaatatat
      241 tattttgatt cctagttcat attttaaaag tgtaattgat aaaagactaa aatatataat
      301 actgcatgaa tatgctcatg ctaaaaatag agatacttta catttaatta tctttaatat
      361 cttctctatt attatgagtt acaatccatt agtacatatt gtaaaaagaa agataattca
      421 tgacaatgaa gtagaagccg atagatttgt ccttaataat attaacaaaa atgaatttaa
      481 aacttatgcg gaatctatta tggactccgt attaaaaacc ccattttcca acaaaaatat
      541 attaagccat tcatttaatg gtaaaaaatc attactcaaa agtagattaa ttaatataaa
      601 agaagccgat ctgaaaaagc agtcaaaatt aattctaatt tttatttgta tttttacttt
      661 ttttatcatg ataattcaaa gccagttttt aatggggcaa tccttaactg attataatta
      721 taaaaagccc ttacaaagcg attaccaaat tttagatgaa agtaaaaatt ttggttcaaa
      781 tagtggatct ttcgttatgt acagcatgaa gaaagacaaa tactatattt ataatgaaaa
      841 agaaagtaga aaaagatatt ctcctgattc aacttacaaa atttatttag cactgtttgg
      901 acttgaccgt catattataa gtgataaaaa ttcacgtatg tcatggaatc ataaacatta
      961 tcttttcgag tcttggaata aggaacaaga tttaaataca gcaatgcaaa attcagttaa
     1021 ttggtacttc gaacgtatta gtaatcaaat accaaagaac tatactgcgg ctcaactcaa
     1081 gcaattaaat tatggtaatg aaaatttggg aagttataaa agctattgga tggaagatag
     1141 tttgaaaata tctaatcttg aacaagtaat agtttttaaa aatatgatgg aacaaaataa
     1201 ccattttagt aaaaaagcaa agaatcaatt atcttcttca ttattgatta agaaaaatga
     1261 aaagtatgaa ctgtatggga aaacaggtac aggtatagta aacgggaagt ataataatgg
     1321 gtggtttgta ggttacgtaa ttacaaatca tgataagtat tattttgcta cacatttatc
     1381 agatggaaac ccgtctggga aaaatgctga attaataagt gaaaaaatat taaaaggaat
     1441 gggtgtttta aatgaccaat aagcaagttg aaatatctat ggctgaatgg gatgttatga
     1501 atataatatg ggataaaaaa tcagtatcag ctaatgaaat tgtagttgaa attcaaaaat
     1561 ataaagaagt tagtgataaa acgattagaa cattaatcac aagactatat aaaaaagaga
     1621 ttataaaacg atacaaatca gaaaatattt atttttactc atcaaatatt aaagaagacg
     1681 atattaaaat gaaaactgct aaaacctttc ttaataaact gtatggaggg gatatgaaaa
     1741 gtttagtgct gaattttgcg aaaaatgaag aattaaataa caaagaaatt gaagaattgc
     1801 gagacatttt aaatgatatt agtaaaaagt aattagaggt atgtttcaaa cataatacat
     1861 atgtaaacca tacttgactc ttattaaatt agttttgtta atgacattta aaatatatta
     1921 ttaaagatga gagtgtccag aaaataacca ttttctgtac actcttgttt tttgtctaaa
     1981 taagtaagta ttacatagaa ttatgtgttc aaaaacccaa caaaaaatct atgttcacaa
     2041 aagacctcgt aatcaagtta tggtacacta aaggtgtata taggaggtct gtattttgaa
     2101 aataggttat gcaagagttt caactggatt acaaaatttg aatttacagg aggatcgatt
     2161 aaatacatat ggttgtgaaa agatatttaa tgaccatatg agtggttcaa aaagtaagcg
     2221 acctggttta gataaagcaa ttgaatttgc gaggtcagga gatacgattg ttgtttggag
     2281 attagataga ctaggacgga acatggagga tttaatcacg ttagttaatg aacttaacga
     2341 acgaggtgtg agttttcata gtttagaaga aaatataacg atggataaat caagttctac
     2401 aggacaatta ttatttcatt tattcgcagc atttgcagag tttgaacgta atttaatttt
     2461 agagcgatca tcagccggtc gaattgcagc acgtgctaga ggccgatatg gaggtagacc
     2521 tgaaaaatta aataaacaag atttaaactt acttaagaca ctttatgata atggtacgcc
     2581 aattaaaaca attgcagagc agtggcaagt ttcacgtaca actatttatc gttatctcaa
     2641 taaattagag gcaaaagaga ataaaaacaa gtagagcaac taatctagct tactataatg
     2701 aatctactaa ataattttat atatcattaa catacaaaga ctttgattca atgtcactcg
     2761 atgtaaaaga gacgttctga cagcgtctaa aatctcataa tattttcctg ttatgagatt
     2821 ttaataataa cacaagaaaa aagcgtacaa attcgtacgc ttttttaata taatacctgt
     2881 ttcactaatg aacgtttata acgcaatcat gatgagagta ttaatacagt taattatttg
     2941 aaatacttta gtttacataa tcgatgatta ccagaagttc tcactatata aaagacacta
     3001 aaattttata acttttagtg cctaatcaat tattttagct accaaacaaa cgactccaaa
     3061 accccttttt aggttccgtt tcttcactct tttcttctat atcactatca ttgtcattta
     3121 gattagctgg aacgtcttct ttttttgctt cttcaccttg tgtactttcg tcgctttgac
     3181 ttccagatat atccttatat tgaatctctg tttctttagt ttcttcctga tctctgttaa
     3241 tatttacagg attagtagta tatgtttttt cttgtgcatc aacgttttgt ctagtgttag
     3301 tagatgtatc aaagggataa ttaaggtgtt tttctttttc taattgattt tctaattttt
     3361 gaatcttttt attactctct aatgccagaa cttgctgatt ttcaagcaat tttgatcttt
     3421 cttgcaactc ttttctgtaa aattcaattg ttgtttcatg acgactttct tgtttttcaa
     3481 tttgattatt taaagtatca atctgatttt taaggatttc tactatttga gcatcgcttt
     3541 tcgattcaaa attaatatta tccgaccctt ttttttgttt gtcttcagta gtactttcaa
     3601 aaccatattt ctttttattt tcatttactc tttgaatgat tttttctata tcggtatctt
     3661 ctttaataaa cgaagtgttt tcctgtttta ttgtctctat attcaatcgc ttaatattat
     3721 taaaaatcgt ttgcttacta acccctaatt cttcactcaa ctttttcaca cttttcatat
     3781 catcaaccct ttataaaatg tttataaacc cttgataaaa tattataagc ttataatgct
     3841 taataataaa gtgattaata aataaaaata aacatccatc aaccttttta tcaagggttt
     3901 atgaaaggtt tatgatattt atcaagggtt tataaaccct tgataaagct atattttccc
     3961 ttttataagt ttatttaaag tgctataata aacttaatat aacaaaaacc ccaactactg
     4021 gtaatagtcg gggtttggta ataaaacgtt tagaaactgt ttatttatat aattttcgat
     4081 acttgtatta tataatgaat aatatcttag tgcaagcacc taaatcatta aataaatgta
     4141 aagctaaata aaaacgtttt attacctctt accaaactaa aatatggaaa gaggtttttt
     4201 gtcatgcaaa atcaatattt tacagtgcaa gaaaattaca aagaaagatt ttatcaaatc
     4261 cctaaagtat tctttaccag tgaaaattat aaaaatctaa ctaatgatat gaaaatagcc
     4321 tatgctatct tacgtgatcg cttaaattta tctattaaaa atagctgggt tgatgaagat
     4381 ggtaacattt actttgttta ttcaaatgaa aagctaatgg aaatcttaaa ttgtaaaaaa
     4441 gaaaagttaa caaaaataaa aaaagggtta gaaaacgatg gtctactcat tcaaaaacga
     4501 cgtggtttaa ataaaccaaa tattctatat ttaatgaaac ctattgtcac agaacgtgat
     4561 atttacaaaa tagaaaaaga agaaaacgat gttgaacctt acggcgagaa ggaagttcga
     4621 aaatcgaacg tccagaagtt cgaaaatcga acgtccagaa gttcgaaaat cgaacgtcca
     4681 gaagttcgaa aatcgaacac taatgatact gactttaatg acattgactt taatgatact
     4741 gagaataatg atatgaatga cttgaatgat ataaagtaca aaaatgaaat tagtaatcat
     4801 tcaaatcatt caaatcaatt tacacataat tttgatgata aagaaatgct attgcaagaa
     4861 ttcccagaac aactaactaa ttatttatta aaatatgatt atagagattt agaaatcata
     4921 aaggctgtta ttcttaaagc aaaaaaatca tttaattcaa ggcatgaaga tatgcattac
     4981 atgttagagg acattgagga tgaaatatta acttctttaa aaagacttaa aaaagctata
     5041 catgataggg gagttaaagg ccaaaaggaa acaatcaagt caatgcaagc ttatttaatg
     5101 caaacaattc taactgaact tgaagaaaca catgcccttt atatgcgccg taaaaacatg
     5161 aaacaatata atatctttaa tcaataattc aaatgatttt ataacactcg aagagacctc
     5221 aaaatattcg gttaaatcat ctgatttttt aactgatgat gatgaaaaaa atcaagaaat
     5281 tgtaagtgat ttagaaaaag gtttgtatcg aaaacgtatg ttaagttatg gtggattgct
     5341 taaacaaaaa cataaaattt taaacttaga cgatgtcgaa gatggtaatt tgattaatgc
     5401 aagtgatgaa gataaaacaa cagacgaaga agaaaaagca cattcaatta ccgcaatttg
     5461 gaatttcgaa aagcaaaatt attatttaag acattagtgt tgactaatgt cttttttgtt
     5521 gattttttat aaaaaagtac tgtcttattt ttgtgacaaa tgctgtatgt agtgtcacaa
     5581 aaataagaca aacgcaatat attgtgtcac aaaaataaga cagtacagct ttgtatgata
     5641 tcactttaaa ataccttaaa acccctggaa tttctggctt tgccagacct atcatttttg
     5701 aatgatagca aattctcctt atgctcttac ggagttttta gagaaaaatt aaaaattctc
     5761 gatttttgat aaaaaacgcc ctgcaggaat ttagaaaaac atgtggaagt tttaaaggat
     5821 tttatgctaa tttttaattt gcatgtaaat gggcagtgtt aaaaaattag tcactggttt
     5881 tgctgaaatt tttgtttgtg aaattaccat atatttattt ggctcatatt tgcgttttaa
     5941 gagcttgtat atatttttaa gttaaaaacc tatatgatac cccctaaacc tttaaaatac
     6001 ccccttaaaa ctcaaaataa aggtatttat aatttgagta agtcttgtga taaagttttt
     6061 taaaaaagga gtgtttattt tgaataaaaa cacagtgtta gatgaaggtt acgttataga
     6121 aactatttta aatgttttct ttttacttgg attaattttt atatctcatt tggaaaattt
     6181 atatatatta attccgtatg ttattttgat gggaataaat gctatttatt tggttgttaa
     6241 ggttatgaat ttcaaaaaga ataattagaa tatttgaggg gattttgaaa cgagtttctt
     6301 cttgttttca cactgttttt ttattcctat tggtgttgtt gcttactttt tgtttttcta
     6361 taaagatgat gcttttgacg agatggaaga aaaatatgat tatcatgaag ataataaaaa
     6421 atagacgacg catttatgcc gagaaaattt attgatgttg agaagaaccc ttaactaaac
     6481 ttgcagacga atgtcggcat agcgtgagct attaagccga ccattcgaca agttttggga
     6541 ttgttaaggg ttccgaggct caacgtcaat aaagcaattg gaataaaatc aacctacaga
     6601 gcatccagct gcaaatgcag ttccgcaaac tgcttcacat acaccgccgg ctattggatt
     6661 tacgattgcc catactccac agtagtgagc acatgcataa agtccagcca tactacaagc
     6721 gaatgctttg ccgttccatt tgaatcctgc atatgttacc ggtctgtttt tatcaacttt
     6781 ttcagcctta tattcaacgt aatttttgtt aataagtatt tttccctctt tgttttcagt
     6841 catacttctt ttttcggcaa tgaattttgt gatttttttg ttgtagttat catatattgt
     6901 ttcagaaact acaatttctt ttgttttttc atttttataa gcgttattag ctatgtagcc
     6961 atatgcattt ttatcatttg cttctgtagc tagatatttt aatttagaat ttttgtcttc
     7021 tagctttatt tttttaaatc cttctttttt catttgcttt tcgaattttt gcatatcttc
     7081 atttttattt tgcaattgtt ctagaaaact tatttctttc ttggtcattt gtgatacttc
     7141 gatgtttttg cttttcaatt ttttcttttt gattttctag taaagaactt ttttgttctt
     7201 catttgcgta agcattagaa aaattcgcgc taaataccag taggaacaag gaaattattg
     7261 aaaataattt tataattttc atttgatacc tccaagataa taagatattt taattgtata
     7321 agtaagcgct ttataaaatc aatatttatt ttaatatatt tataaacata tttttgaaag
     7381 gagtctattt tatgaatgaa aatttagaaa aatatataaa aagtttgcca cttttaggtt
     7441 tgttaattag tattttgtta attgttttat tcttttttat tttagatgtt gatgaagatt
     7501 tattcgtggt ttttttatac tgtttattgc ctttgatagt taatttgtct atttatggtt
     7561 tttatgtttt tgttaaaaga aagtaagatt atgaataatt ttgtttttaa tttgtatgct
     7621 ttgagggggt tggaaaacgg taggtgtata gattagattt cttgtttttt tgaaacgagt
     7681 gtgaacgagt ttcttcttgt cttgattttt gataaaaacg ccctacagga atttagaaaa
     7741 acgtggggaa gttttaaaga attttatgct aatttttaat ttgcatgtaa atgggcagtg
     7801 tctaaaaata gttattgttt ttgctcaaat ttttgttttt gaaaatagca tatatttatt
     7861 tggctcatat ttgcgtttta aaagcatata taagttttag atataaaact atatgaatca
     7921 cccctaaatc tttaaaatgc cctcttaaaa ttcaaaataa gggcataaaa aataaaagct
     7981 accccagata aagtgaggta gctttttctt tcatacggtc tatttatttt tctttcgacg
     8041 ccgtatcgtc gatgtaattc aaaaaagtta tgggctataa atctacatca ctttttcaag
     8101 aatgtagtag caatattcaa cgcaaattaa tttttataac tgctcggaat atctcaagcc
     8161 gtttctttaa ttttgaaata aaaaaatcga cgaaggtcga ttacgttttt gcacatgtcg
     8221 atttatccga cgtataagtg cgcccttacg ggatttaact agattataac gacgaatttt
     8281 agacctgtaa agcaataaca attaggtcta ccaggtagat aaagtgtaca aagtagaacc
     8341 gaaaaagtta tttattttga agatttttcg tagatataat ttgctaataa acttcctaaa
     8401 actgaataaa gaaaaatagt taaactcatg taatcactcc ataattctga agtgtctcca
     8461 tgtctacgca tagacacgca tttatgccga aaaaatttat tgatgttgag aagaaccctt
     8521 aactaaactt gcagacgaat gtcggcatag cgtgagctat taagccgacc attcgacaag
     8581 ttttgggatt gttaagggtt ccgaggctca acgtcaataa agcaattgga ataaagcacc
     8641 tatgattatt taaaaatata agttataata cattcaaaaa tatttttgaa tgaggtgtat
     8701 tatgattcaa acggttgttg ctgctgctgt tctttatatt gctacagcag ttgatttatt
     8761 agtaatttta ttaatatttt ttgctagagc aaagactaga aaagaatata gagatattta
     8821 tgttggtcaa tatttagggt ctattatttt aatattagtt agtttgtttt tagcttttgt
     8881 attaaattat gttccagaaa agtggatatt aggtttattg ggtttaatac caatttatct
     8941 tggaattaaa gtggctattt atgatgattg tgaaggagaa aagagagcta aaaaagaatt
     9001 gaatgaaaaa ggattgtcta aattagttgg tacggttgca attgttacga tagcaagttg
     9061 tggcgccgat aatattggtt tatttgttcc gtattttgtg acattaagtg ttactaattt
     9121 attacttact ctgtttgtct ttttaatttt aattttcttc ttggtgttta ctgcacaaaa
     9181 attagctaat attccaggaa ttggagaaat tgttgagaaa tttagtcgtt ggattatggc
     9241 tattatttat atagctttag gtttatttat tattattgaa aatgacacta ttcaaacaat
     9301 tttaggattt atattttaat ttagggtgtg attttatatg agttatgaaa atacttgtga
     9361 tgtgatctgt gtacatgagg ataaagttaa caatgcttta agttttttag aagatgataa
     9421 atctaagaaa ttacttaaca ttttagaaaa aatttgtgat gagaagaaat tgaaaatcat
     9481 attatctttg attaaagaag atgagttgtg tgtttgtgat atttctttga tattgaaaat
     9541 gagtgttgct tcaacttcac atcatttaag gcttttatat aaaaatgagg tacttgattt
     9601 ttataaagat ggaaagatgg catattattt tattaaagac gatgaaataa gagagttttt
     9661 ctctaaaaat catgagggtt tttgaaacga gtttcttctt gtcttgatac tataaaaaac
     9721 aaagttatat aacataagac gacttatcgg aagtagatat gcttgtatat tcacagatgg
     9781 tataataata gagtcgccta tctctcaggc gtcaattgac gaagagagga ggtgcattac
     9841 tttgctaatc attttcgttc acatcatagc accagtcatc agtggctgtg ctgttgcgta
     9901 ttatacttat tggcttagta aacgcaacaa ataattaata ggcgacttta taagccagca
     9961 tcgaaaaaat ccccacacta ctgcaatagt gtggggattg gtgcattaat tgttaatcat
    10021 tttcgttata taaattataa cactggttag tagagaaatg caaaatgatt gaattaatag
    10081 taaatgtatt aatgaatact cacacttata cttttaaaat agttcttcct aaatgttgtc
    10141 cttttaaaag ctgatctatc gttttataga attcttcaaa tccaatttca ttgtatagaa
    10201 gtgaatttcc tatattccat tcatttgcta acttactcca tatatcttca cggatattat
    10261 gatcaacata tacagaatca atgccaatta atttaatacc tctaagaata aacggtaata
    10321 tattagcgtt aagtgaattt cctgctacat ttccacatac agtagcaaca ccttgataat
    10381 ttagacgttt taaaccgtat aatgttacat tactccccac tgtatctaag aaataatcaa
    10441 aaataccttt tttaagcggt ttattatcat cttcaattat tattacttgt tttgcaccta
    10501 atttttttgc gatttctatt tgcttttctt ttcttacgat agcagtgata ttttcatatc
    10561 caattttatg taaaatttgt atagcaacac tgcccacacc acctgtagcc ccagacacta
    10621 aaatttcagg ttgtgatttg atattcattc catatttttc tagttcaaaa attgataatg
    10681 ctgccgtaaa accggctgtg ccatatacca tagactcttt aatagacatt tcttggggaa
    10741 taggaacaat ccactctcca ggcacttgtg catattctga aaatccacct gtatgtgtca
    10801 cgccaacatc ataaccagta actaatacat tatcgccttc tttaaactta tcattcgaac
    10861 ttgattcaac aatacctgca aaatctatac caggaatcat aggataatct ctaattacac
    10921 cgcctttata ttgtaaagct agcatatcct tataatttat tgaagagtat accactttta
    10981 ctaatacatc acccttcgat aaatgagatt catcaacttt ttctacttta taactaacat
    11041 ttgatgcact ttttctcact actaccgcat tgaacattta ttagccttct ttcacaattg
    11101 ttttgtagaa gtatgttttt gttgcaaaac tattttccta caaaacttat catgatatga
    11161 taagcgagtc aatactaatg atttaagaga ggttttagtt gtggaattag ataattgtat
    11221 aaattatttg ttgagtactt ctcagaatag agtttttaaa cattttaaaa gtttattaag
    11281 tccctataat attactccag cagaatatgg tgttttgaat tgcttgatta atagtaattt
    11341 gaatacacct aaagcgattg gtaatacttt aaatttagaa ccttcaacaa tatctggaat
    11401 attagataag atgtccaaaa aggatctaat aacacgtcaa actgataatg aaaatagaag
    11461 aactgtgcta ataaacagta caaataaagc aaaagactta tggccaacat tagaaaagaa
    11521 agcctatcaa cttaataatc attacttcgc taatttaact aatgaagaaa ctgtagtgtt
    11581 taaaaagata ttactaaaga taaataaaac tacgttttaa tttttacgtt ataaaaaagt
    11641 tgaagtgcga attataatcg agtaaatata aatgatagct tagcttaatt atatattaaa
    11701 gccaactata cttttattcc cgaaaaacct actatccaaa tttctaagat actttagcct
    11761 ctataataaa catattgtaa tataatttat gtattattta ggtttaccct aatcgaacaa
    11821 taaaaaatac ttgtaaaatg ctattgtcac tttgcttgat atatgagaat tatttaacct
    11881 tataaatgag aaaaaagcaa cgcactttaa ataagatacg ttgctttttc gattgatgaa
    11941 cacctataat taaactattc atctattatt tatgattttt tgtatataca atatttctag
    12001 tttgttaaag agaattaaga aaataaatct cgaaaataat aaagggaaaa tcagtttttg
    12061 atatcaaaat tatacatgtc aacgataata caaaatataa tacaaactat aagatgttat
    12121 cagtatttat tatgcattta gaatattaaa attccttcat tacactcttg gcggtttcac
    12181 ttatcaactt atcatttggc ttatcacttt tattgtcttt attcgtaaaa atgactaaaa
    12241 caataggttc agattggccc ttaggataaa caaaagcaac atcatttcta gaagcatatg
    12301 ttattgcttg accactttta tcagcaacct tatagtcttt tggaacacca tctttaatta
    12361 aagtgtctcc gtttttatta ttaaacatta aatcaagtaa gaaattttta ttttttttgc
    12421 ttaattttcc atttgcgata agtttattta aagtcttgcc gaaagcagca ggcgttgaag
    12481 tatctttttt gctctttggt gaatagtaat ttaattctat ctcatatcta actggatttg
    12541 ttactttatc tcctagttct tttagacgtt gtttaacttt tttgattcca ccgatttctt
    12601 ttataatttt attgtttgct gtattatcac tatatgtcat tgaagcctca ataagttctt
    12661 ttaaagtgat atcttttcct acatattttt ctaaaatagg agaataagca actatatcat
    12721 ctttgttaat atgtactttt ttatttaact tattataagg tacttgttct aacaaaatag
    12781 cactatttat cgcttttgaa gtcgaagcat aggcaaatct cttatctgaa ttaaatttta
    12841 cttccttacc acttttagta tttaaagcat aaacaccaat atgagcatta tatttttttt
    12901 ctaaatcatt taactctttg gcatgtgaac tgtttgaatt acatgcactt aaaactaaag
    12961 caattacaat taaaaatatt aactttttca aaataaaccc tccgatatta cagttgtaat
    13021 ttttataaat caataatatt acaattgtaa tattgatgtc aattgtttat tataaatatt
    13081 tgaaagaagg tgtcaaaatg gctaaattac taataatgag cgtagtaagc ttttgtttta
    13141 tatttctatt gttagtattt tttaggtaca tattaaaacg ctattttaat tatagtttaa
    13201 attataaagt ttggtatcta actatgcttg ctggattaat tccttttatc ccaattaaat
    13261 tctcttttat aaaatttaat aatgtgaata atcaagcgcc cacagttgaa agtaagtcac
    13321 acgatttgaa ccataacata aataccacca aacctattca agagttcaca acagatatcc
    13381 ataagtttaa ttg
//
