LOCUS       NZ_CP062332            13162 bp    DNA     circular CON 13-MAY-2024
DEFINITION  Staphylococcus aureus strain NAS_OP_017 plasmid unnamed1, complete
            sequence.
ACCESSION   NZ_CP062332
VERSION     NZ_CP062332.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN15567977
            Assembly: GCF_022404675.1
KEYWORDS    RefSeq.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 13162)
  AUTHORS   Cella,E., Sutcliffe,C., Jubair,M., Weatherholtz,R.C., Reid,R.,
            Reid,A., Quay,D., Azarian,T.H. and Hammitt,L.
  TITLE     Carriage prevalence and genomic epidemiology of Staphylococcus
            aureus among Native Americans in the southwest US
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 13162)
  AUTHORS   Cella,E., Sutcliffe,C., Jubair,M., Weatherholtz,R.C., Reid,R.,
            Reid,A., Quay,D., Azarian,T.H. and Hammitt,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JUL-2020) Burnett School of Biomedical Sciences,
            University of central Florida, 4111 Libra Dr, Orlando, FL 32816, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 2020
            Assembly Method                   :: Uniclycler v. 0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 50x
            Sequencing Technology             :: Illumina MiSeq; Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_022404675.1-RS_2024_05_13
            Annotation Date                   :: 05/13/2024 03:58:30
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,854
            CDSs (total)                      :: 2,772
            Genes (coding)                    :: 2,695
            CDSs (with protein)               :: 2,695
            Genes (RNA)                       :: 82
            rRNAs                             :: 7, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 42 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 15 of 77
            Pseudo Genes (multiple problems)  :: 16 of 77
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP062332.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..13162
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /strain="NAS_OP_017"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /plasmid="unnamed1"
                     /collected_by="UCF"
     gene            complement(339..917)
                     /locus_tag="IF760_RS14170"
                     /old_locus_tag="IF760_14145"
     CDS             complement(339..917)
                     /locus_tag="IF760_RS14170"
                     /old_locus_tag="IF760_14145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000690624.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0000150 - DNA strand exchange activity [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="WP_000690637.1"
                     /translation="MKIGYARVSTGLQNLNLQEDRLNTYGCEKIFNDHMSGSKSKRPGL
                     DKAIEFARSGDTIVVWRLDRLGRNMEDLITLVNELNERGVSFHSLEENITMDKSSSTGQ
                     LLFHLFAAFAEFERNLILERSSAGRIAARARGRYGGRPEKLNKQDLNLLKTLYDNGTPI
                     KTIAEQWQVSRTTIYRYLNKLEAKENKNK"
                     /name="recombinase family protein"
     gene            complement(1181..1561)
                     /gene="blaI"
                     /locus_tag="IF760_RS14175"
                     /old_locus_tag="IF760_14150"
     ARG             complement(1185..1561)
                     /name="blaI"
                     /gene="blaI"
                     /gene_name="penicillinase repressor BlaI"
                     /tool="amrfinderplus"
                     /drug_class="BETA-LACTAM"
                     /identity="98.41"
                     /coverage="100.0"
     gene            complement(1551..3308)
                     /gene="blaR1"
                     /locus_tag="IF760_RS14180"
                     /old_locus_tag="IF760_14155"
     ARG             complement(1555..3308)
                     /name="blaR1"
                     /gene="blaR1"
                     /gene_name="beta-lactam sensor/signal transducer BlaR1"
                     /tool="amrfinderplus"
                     /drug_class="BETA-LACTAM"
                     /identity="95.38"
                     /coverage="100.0"
     gene            3415..4260
                     /gene="blaZ"
                     /locus_tag="IF760_RS14185"
                     /old_locus_tag="IF760_14160"
     ARG             3416..4257
                     /name="blaZ"
                     /gene="blaZ"
                     /gene_name="penicillin-hydrolyzing class A beta-lactamase
                     BlaZ"
                     /tool="amrfinderplus"
                     /drug_class="BETA-LACTAM"
                     /identity="100.0"
                     /coverage="100.0"
     gene            complement(4786..5205)
                     /locus_tag="IF760_RS14190"
                     /old_locus_tag="IF760_14165"
     CDS             complement(4786..5205)
                     /locus_tag="IF760_RS14190"
                     /old_locus_tag="IF760_14165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000418034.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0003700 - DNA-binding transcription factor activity
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="WP_000418035.1"
                     /translation="MELDNCINYLLSTSQNRVFKHFKSLLSPYNITPAEYGVLNCLINS
                     NLNTPKAIGNTLNLEPSTISGILDKMSKKDLITRQTDNENRRTVLINSTNKAKDLWPTL
                     EKKAYQLNNHYFANLTNEETVVFKKILLKINKTTF"
                     /name="MarR family transcriptional regulator"
     gene            5329..6300
                     /locus_tag="IF760_RS14195"
                     /old_locus_tag="IF760_14170"
     CDS             5329..6300
                     /locus_tag="IF760_RS14195"
                     /old_locus_tag="IF760_14170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000481285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="WP_000481279.1"
                     /translation="MFNAVVVRKSASNVSYKVEKVDESHLSKGDVLVKVVYSSINYKDM
                     LALQYKGGVIRDYPMIPGIDFAGIVESSSNDKFKEGDNVLVTGYDVGVTHTGGFSEYAQ
                     VPGEWIVPIPQEMSIKESMVYGTAGFTAALSIFELEKYGMNIKSQPEILVSGATGGVGS
                     VAIQILHKIGYENITAIVRKEKQIEIAKKLGAKQVIIIEDDNKPLKKGIFDYFLDTVGS
                     NVTLYGLKRLNYQGVATVCGNVAGNSLNANILPFILRGIKLIGIDSVYVDHNIREDIWS
                     KLANEWNIGNSLLYNEIGFEEFYKTIDQLLKGQHLGRTILKV"
                     /name="oxidoreductase"
     gene            complement(6472..6564)
                     /locus_tag="IF760_RS14200"
                     /old_locus_tag="IF760_14175"
     CDS             complement(6472..6564)
                     /locus_tag="IF760_RS14200"
                     /old_locus_tag="IF760_14175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002495832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="WP_001791613.1"
                     /translation="MLIIFVHIIAPVISGCAVAYYTYWLSKRNK"
                     /name="type I toxin-antitoxin system Fst family toxin"
     gene            complement(6721..7068)
                     /locus_tag="IF760_RS14205"
                     /old_locus_tag="IF760_14180"
     CDS             complement(6721..7068)
                     /locus_tag="IF760_RS14205"
                     /old_locus_tag="IF760_14180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020366422.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0003700 - DNA-binding transcription factor activity
                     [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="WP_000119690.1"
                     /translation="MSYENTCDVICVHEDKVNNALSFLEDDKSKKLLNILEKICDEKKL
                     KIILSLIKEDELCVCDISLILKMSVASTSHHLRLLYKNEVLDFYKDGKMAYYFIKDDEI
                     REFFSKNHEGF"
                     /name="metalloregulator ArsR/SmtB family transcription
                     factor"
     gene            complement(7087..7704)
                     /gene="cadD"
                     /locus_tag="IF760_RS14210"
                     /old_locus_tag="IF760_14185"
     ARG             complement(7091..7704)
                     /name="cadD"
                     /gene="cadD"
                     /gene_name="cadmium resistance transporter CadD"
                     /tool="amrfinderplus"
                     /drug_class="CADMIUM"
                     /identity="100.0"
                     /coverage="100.0"
     gene            complement(8821..9015)
                     /locus_tag="IF760_RS14215"
                     /old_locus_tag="IF760_14190"
     CDS             complement(8821..9015)
                     /locus_tag="IF760_RS14215"
                     /old_locus_tag="IF760_14190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001004925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001004925.1"
                     /translation="MNENLEKYIKSLPLLGLLISILLIVLFFFILDVDEDLFVVFLYCL
                     LPLIVNLSIYGFYVFVKRK"
                     /name="hypothetical protein"
     gene            9125..9817
                     /locus_tag="IF760_RS14220"
                     /old_locus_tag="IF760_14195"
     CDS             9125..9817
                     /locus_tag="IF760_RS14220"
                     /old_locus_tag="IF760_14195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000691527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative immunity/bacteriocin fusion bifunctional
                     protein"
                     /protein_id="WP_000691527.1"
                     /translation="MKIIKLFSIISLFLLVFSANFSNAYANEEQKSSLLENQKEKLKSK
                     NIEVSQMTKKEISFLEQLQNKNEDMQKFEKQMKKEGFKKIKLEDKNSKLKYLATEANDK
                     NAYGYIANNAYKNEKTKEIVVSETIYDNYNKKITKFIAEKRSMTENKEGKILINKNYVE
                     YKAEKVDKNRPVTYAGFKWNGKAFACSMAGLYACAHYCGVWAIVNPIAGGVCEAVCGTA
                     FAAGCSVG"
                     /name="putative immunity/bacteriocin fusion bifunctional
                     protein"
     gene            complement(9985..10134)
                     /locus_tag="IF760_RS14225"
                     /old_locus_tag="IF760_14200"
     CDS             complement(9985..10134)
                     /locus_tag="IF760_RS14225"
                     /old_locus_tag="IF760_14200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000695367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000695366.1"
                     /translation="MKIKTFATFTHRQLDKQVNRFLKNDIHVLEIQTHSNWLYISAIVF
                     YEDK"
                     /name="hypothetical protein"
     gene            complement(10678..>10968)
                     /locus_tag="IF760_RS14230"
                     /old_locus_tag="IF760_14205"
                     /pseudo=""
     CDS             complement(10678..>10968)
                     /locus_tag="IF760_RS14230"
                     /old_locus_tag="IF760_14205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009384749.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006260 - DNA replication [Evidence IEA]"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /name="protein rep"
     gene            complement(10988..11971)
                     /locus_tag="IF760_RS14235"
                     /old_locus_tag="IF760_14210"
     CDS             complement(10988..11971)
                     /locus_tag="IF760_RS14235"
                     /old_locus_tag="IF760_14210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001181101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="WP_001181107.1"
                     /translation="MQNQYFTVQENYKERFYQIPKVFFTSENYKNLTNDMKIAYAILRD
                     RLNLSIKNSWVDEDGNIYFVYSNEKLMEILNCKKEKLTKIKKGLENDGLLIQKRRGLNK
                     PNILYLMKPIVTERDIYKIEKEENDVEPYGEKEVRKSNVQKFENRTSRSSKIERPEVRK
                     SNTNDTDFNDIDFNDTENNDMNDLNDIKYKNEISNHSNHSNQFTHNFDDKEMLLQEFPE
                     QLTNYLLKYDYRDLEIIKAVILKAKKSFNSRHEDMHYMLEDIEDEILTSLKRLKKAIHD
                     RGVKGQKETIKSMQAYLMQTILTELEETHALYMRRKNMKQYNIFNQ"
                     /name="replication initiator protein A"
     gene            12397..13140
                     /locus_tag="IF760_RS14240"
                     /old_locus_tag="IF760_14215"
     CDS             12397..13140
                     /locus_tag="IF760_RS14240"
                     /old_locus_tag="IF760_14215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000843735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="WP_000843732.1"
                     /translation="MKSVKKLSEELGVSKQTIFNNIKRLNIETIKQENTSFIKEDTDIE
                     KIIQRVNENKKKYGFESTTEDKQKKGSDNINFESKSDAQIVEILKNQIDTLNNQIEKQE
                     SRHETTIEFYRKELQERSKLLENQQVLALESNKKIQKLENQLEKEKHLNYPFDTSTNTR
                     QNVDAQEKTYTTNPVNINRDQEETKETEIQYKDISGSQSDESTQGEEAKKEDVPANLND
                     NDSDIEEKSEETEPKKGFWSRLFGS"
                     /name="DUF536 domain-containing protein"
     ARG             3416..4260
                     /name="PC1 beta-lactamase (blaZ)"
                     /gene="PC1 beta-lactamase (blaZ)"
                     /gene_name="BlaZ beta-lactamase"
                     /tool="rgi"
                     /drug_class="penam"
                     /identity="96.8"
                     /coverage="100.0"
     Typing          complement(10476..10623)
                     /name="MOBV"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="95.973"
                     /coverage="100.0"
                     /evalue="6.06e-62"
     Typing          complement(10681..10857)
                     /name="rep_cluster_1017"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="93.258"
                     /coverage="94.0"
                     /evalue="6.2e-68"
     Typing          complement(10989..11971)
                     /name="rep_cluster_2100"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="99.593"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          12398..13140
                     /name="rep_cluster_1733"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 gttataaaat tttagtgtct tttatatagt gagaacttct ggtaatcatc gattatgtaa
       61 actaaagtat ttcaaataat taactgtatt aatactctca tcatgattgc gttataaacg
      121 ttcattagtg aaacaggtat tatattaaaa aagcgtacga atttgtacgc ttttttcttg
      181 tgttattatt aaaatctcat aacaggaaaa tattatgaga ttttagacgc tgtcagaacg
      241 tctcttttac atcgagtgac attgaatcaa agtctttgta tgttaatgat atataaaatt
      301 atttagtaga ttcattatag taagctagat tagttgctct acttgttttt attctctttt
      361 gcctctaatt tattgagata acgataaata gttgtacgtg aaacttgcca ctgctctgca
      421 attgttttaa ttggcgtacc attatcataa agtgtcttaa gtaagtttaa atcttgttta
      481 tttaattttt caggtctacc tccatatcgg cctctagcac gtgctgcaat tcgaccggct
      541 gatgatcgct ctaaaattaa attacgttca aactctgcaa atgctgcgaa taaatgaaat
      601 aataattgtc ctgtagaact tgatttatcc atcgttatat tttcttctaa actatgaaaa
      661 ctcacacctc gttcgttaag ttcattaact aacgtgatta aatcctccat gttccgtcct
      721 agtctatcta atctccaaac aacaatcgta tctcctgacc tcgcaaattc aattgcttta
      781 tctaaaccag gtcgcttact ttttgaacca ctcatatggt cattaaatat cttttcacaa
      841 ccatatgtat ttaatcgatc ctcctgtaaa ttcaaatttt gtaatccagt tgaaactctt
      901 gcataaccta ttttcaaaat acagacctcc tatatacacc tttagtgtac cataacttga
      961 ttacgaggtc ttttgtgaac atagattttt tgttgggttt ttgaacacat aattctatgt
     1021 aatacttact tatttagaca aaaaacaaga gtgtacagaa aatggttatt ttctggacac
     1081 tctcatcttt aataatatat tttaaatgtc attaacaaaa ctaatttaat aagagtcaag
     1141 tatggtttac atatgtatta tgtttgaaac atacctctaa ttacttttta ctaatatcat
     1201 ttaaaatgtc tcgcaattct tcaatttctt tgttatttaa ttcttcattt ttcgcaaaat
     1261 tcagcactaa acttttcata tcccctccat acagtttatt aagaaaggtt ttagcagttt
     1321 tcattttaat atcgtcttct ttaatatttg atgagtaaaa ataaatattt tctgatttgt
     1381 atcgttttat aatctctttt ttatatagtc ttgtgattaa tgttctaatc gttttatcac
     1441 taacttcttt atatttttga atttcaacta caatttcatt agctgatact gattttttat
     1501 cccatattat attcataaca tcccattcag ccatagatat ttcaacttgc ttattggtca
     1561 tttaaaacac ccattccttt taatattttt tcacttatta attcagcatt tttcccagac
     1621 gggtttccat ctgataaatg tgtagcaaaa taatacttat catgatttgt aattacgtaa
     1681 cctacaaacc acccattatt atacttcccg tttactatac ctgtacctgt tttcccatac
     1741 agttcatact tttcattttt cttaatcaat aatgaagaag ataattgatt ctttgctttt
     1801 ttactaaaat ggttattttg ttccatcata tttttaaaaa ctattacttg ttcaagatta
     1861 gatattttca aactatcttc catccaatag cttttataac ttcccaaatt ttcattacca
     1921 taatttaatt gcttgagttg agccgcagta tagttctttg gtatttgatt actaatacgt
     1981 tcgaagtacc aattaactga attttgcatt gctgtattta aatcttgttc cttattccaa
     2041 gactcgaaaa gataatgttt atgattccat gacatacgtg aatttttatc acttataata
     2101 tgacggtcaa gtccaaacag tgctaaataa attttgtaag ttgaatcagg agaatatctt
     2161 tttctacttt ctttttcatt ataaatatag tatttgtctt tcttcatgct gtacataacg
     2221 aaagatccac tatttgaacc aaaattttta ctttcatcta aaatttggta atcgctttgt
     2281 aagggctttt tataattata atcagttaag gattgcccca ttaaaaactg gctttgaatt
     2341 atcatgataa aaaaagtaaa aatacaaata aaaattagaa ttaattttga ctgctttttc
     2401 agatcggctt cttttatatt aattaatcta cttttgagta atgatttttt accattaaat
     2461 gaatggctta atatattttt gttggaaaat ggggttttta atacggagtc cataatagat
     2521 tccgcataag ttttaaattc atttttgtta atattattaa ggacaaatct atcggcttct
     2581 acttcattgt catgaattat ctttcttttt acaatatgta ctaatggatt gtaactcata
     2641 ataatagaga agatattaaa gataattaaa tgtaaagtat ctctattttt agcatgagca
     2701 tattcatgca gtattatata ttttagtctt ttatcaatta cacttttaaa atatgaacta
     2761 ggaatcaaaa taatatattt cccataccaa aaagttattg gagattgaat agcctctgct
     2821 tttcgaatca caatattttt tttatattga tggttgaaaa gtatcgtatc tattttattt
     2881 ttttcatttt cgtttagata aagtgactgt ttctttaaat atttaagata taataaggct
     2941 ttcaaaaatt taaaacttaa aataataact aaaactatcc aaataactgt gcagatatta
     3001 tcaattgaat cccaattaaa cttatggata tctgttgtga actcttgaat aggtttggtg
     3061 gtatttatgt tatggttcaa atcgtgtgac ttactttcaa ctgtgggcgc ttgattattc
     3121 acattattaa attttataaa agagaattta attgggataa aaggaattaa tccagcaagc
     3181 atagttagat accaaacttt ataatttaaa ctataattaa aatagcgttt taatatgtac
     3241 ctaaaaaata ctaacaatag aaatataaaa caaaagctta ctacgctcat tattagtaat
     3301 ttagccattt tgacaccttc tttcaaatat ttataataaa caattgacat caatattaca
     3361 attgtaatat tattgattta taaaaattac aactgtaata tcggagggtt tattttgaaa
     3421 aagttaatat ttttaattgt aattgcttta gttttaagtg catgtaattc aaacagttca
     3481 catgccaaag agttaaatga tttagaaaaa aaatataatg ctcatattgg tgtttatgct
     3541 ttaaatacta aaagtggtaa ggaagtaaaa tttaattcag ataagagatt tgcctatgct
     3601 tcgacttcaa aagcgataaa tagtgctatt ttgttagaac aagtacctta taataagtta
     3661 aataaaaaag tacatattaa caaagatgat atagttgctt attctcctat tttagaaaaa
     3721 tatgtaggaa aagatatcac tttaaaagaa cttattgagg cttcaatgac atatagtgat
     3781 aatacagcaa acaataaaat tataaaagaa atcggtggaa tcaaaaaagt taaacaacgt
     3841 ctaaaagaac taggagataa agtaacaaat ccagttagat atgagataga attaaattac
     3901 tattcaccaa agagcaaaaa agatacttca acgcctgctg ctttcggcaa gactttaaat
     3961 aaacttatcg caaatggaaa attaagcaaa aaaaataaaa atttcttact tgatttaatg
     4021 tttaataata aaaacggaga cactttaatt aaagatggtg ttccaaaaga ctataaggtt
     4081 gctgataaaa gtggtcaagc aataacatat gcttctagaa atgatgttgc ttttgtttat
     4141 cctaagggcc aatctgaacc tattgtttta gtcattttta cgaataaaga caataaaagt
     4201 gataagccaa atgataagtt gataagtgaa accgccaaga gtgtaatgaa ggaattttaa
     4261 tattctaaat gcataataaa tactgataac atcttatagt ttgtattata ttttgtatta
     4321 tcgttgacat gtataatttt gatatcaaaa actgattttc cctttattat tttcgagatt
     4381 tattttctta attctcttta acaaactaga aatattgtat atacaaaaaa tcataaataa
     4441 tagatgaata gtttaattat aggtgttcat caatcgaaaa agcaacgtat cttatttaaa
     4501 gtgcgttgct tttttctcat ttataaggtt aaataattct catatatcaa gcaaagtgac
     4561 aatagcattt tacaagtatt ttttattgtt cgattagggt aaacctaaat aatacataaa
     4621 ttatattaca atatgtttat tatagaggct aaagtatctt agaaatttgg atagtaggtt
     4681 tttcgggaat aaaagtatag ttggctttaa tatataatta agctaagcta tcatttatat
     4741 ttactcgatt ataattcgca cttcaacttt tttataacgt aaaaattaaa acgtagtttt
     4801 atttatcttt agtaatatct ttttaaacac tacagtttct tcattagtta aattagcgaa
     4861 gtaatgatta ttaagttgat aggctttctt ttctaatgtt ggccataagt cttttgcttt
     4921 atttgtactg tttattagca cagttcttct attttcatta tcagtttgac gtgttattag
     4981 atcctttttg gacatcttat ctaatattcc agatattgtt gaaggttcta aatttaaagt
     5041 attaccaatc gctttaggtg tattcaaatt actattaatc aagcaattca aaacaccata
     5101 ttctgctgga gtaatattat agggacttaa taaactttta aaatgtttaa aaactctatt
     5161 ctgagaagta ctcaacaaat aatttataca attatctaat tccacaacta aaacctctct
     5221 taaatcatta gtattgactc gcttatcata tcatgataag ttttgtagga aaatagtttt
     5281 gcaacaaaaa catacttcta caaaacaatt gtgaaagaag gctaataaat gttcaatgcg
     5341 gtagtagtga gaaaaagtgc atcaaatgtt agttataaag tagaaaaagt tgatgaatct
     5401 catttatcga agggtgatgt attagtaaaa gtggtatact cttcaataaa ttataaggat
     5461 atgctagctt tacaatataa aggcggtgta attagagatt atcctatgat tcctggtata
     5521 gattttgcag gtattgttga atcaagttcg aatgataagt ttaaagaagg cgataatgta
     5581 ttagttactg gttatgatgt tggcgtgaca catacaggtg gattttcaga atatgcacaa
     5641 gtgcctggag agtggattgt tcctattccc caagaaatgt ctattaaaga gtctatggta
     5701 tatggcacag ccggttttac ggcagcatta tcaatttttg aactagaaaa atatggaatg
     5761 aatatcaaat cacaacctga aattttagtg tctggggcta caggtggtgt gggcagtgtt
     5821 gctatacaaa ttttacataa aattggatat gaaaatatca ctgctatcgt aagaaaagaa
     5881 aagcaaatag aaatcgcaaa aaaattaggt gcaaaacaag taataataat tgaagatgat
     5941 aataaaccgc ttaaaaaagg tatttttgat tatttcttag atacagtggg gagtaatgta
     6001 acattatacg gtttaaaacg tctaaattat caaggtgttg ctactgtatg tggaaatgta
     6061 gcaggaaatt cacttaacgc taatatatta ccgtttattc ttagaggtat taaattaatt
     6121 ggcattgatt ctgtatatgt tgatcataat atccgtgaag atatatggag taagttagca
     6181 aatgaatgga atataggaaa ttcacttcta tacaatgaaa ttggatttga agaattctat
     6241 aaaacgatag atcagctttt aaaaggacaa catttaggaa gaactatttt aaaagtataa
     6301 gtgtgagtat tcattaatac atttactatt aattcaatca ttttgcattt ctctactaac
     6361 cagtgttata atttatataa cgaaaatgat taacaattaa tgcaccaatc cccacactat
     6421 tgcagtagtg tggggatttt ttcgatgctg gcttataaag tcgcctatta attatttgtt
     6481 gcgtttacta agccaataag tataatacgc aacagcacag ccactgatga ctggtgctat
     6541 gatgtgaacg aaaatgatta gcaaagtaat gcacctcctc tcttcgtcaa ttgacgcctg
     6601 agagataggc gactctatta ttataccatc tgtgaatata caagcatatc tacttccgat
     6661 aagtcgtctt atgttatata actttgtttt ttatagtatc aagacaagaa gaaactcgtt
     6721 tcaaaaaccc tcatgatttt tagagaaaaa ctctcttatt tcatcgtctt taataaaata
     6781 atatgccatc tttccatctt tataaaaatc aagtacctca tttttatata aaagccttaa
     6841 atgatgtgaa gttgaagcaa cactcatttt caatatcaaa gaaatatcac aaacacacaa
     6901 ctcatcttct ttaatcaaag ataatatgat tttcaatttc ttctcatcac aaattttttc
     6961 taaaatgtta agtaatttct tagatttatc atcttctaaa aaacttaaag cattgttaac
     7021 tttatcctca tgtacacaga tcacatcaca agtattttca taactcatat aaaatcacac
     7081 cctaaattaa aatataaatc ctaaaattgt ttgaatagtg tcattttcaa taataataaa
     7141 taaacctaaa gctatataaa taatagccat aatccaacga ctaaatttct caacaatttc
     7201 tccaattcct ggaatattag ctaatttttg tgcagtaaac accaagaaga aaattaaaat
     7261 taaaaagaca aacagagtaa gtaataaatt agtaacactt aatgtcacaa aatacggaac
     7321 aaataaacca atattatcgg cgccacaact tgctatcgta acaattgcaa ccgtaccaac
     7381 taatttagac aatccttttt cattcaattc ttttttagct ctcttttctc cttcacaatc
     7441 atcataaata gccactttaa ttccaagata aattggtatt aaacccaata aacctaatat
     7501 ccacttttct ggaacataat ttaatacaaa agctaaaaac aaactaacta atattaaaat
     7561 aatagaccct aaatattgac caacataaat atctctatat tcttttctag tctttgctct
     7621 agcaaaaaat attaataaaa ttactaataa atcaactgct gtagcaatat aaagaacagc
     7681 agcagcaaca accgtttgaa tcataataca cctcattcaa aaatattttt gaatgtatta
     7741 taacttatat ttttaaataa tcataggtgc tttattccaa ttgctttatt gacgttgagc
     7801 ctcggaaccc ttaacaatcc caaaacttgt cgaatggtcg gcttaatagc tcacgctatg
     7861 ccgacattcg tctgcaagtt tagttaaggg ttcttctcaa catcaataaa ttttttcggc
     7921 ataaatgcgt cgtctatgcg tagacatgga gacacttcag aattatggag tgattacatg
     7981 agtttaacta tttttcttta ttcagtttta ggaagtttat tagcaaatta tatctacgaa
     8041 aaatcttcaa aataaataac tttttcggtt ctactttgta cactttatct acctggtaga
     8101 cctaattgtt attgctttac aggtctaaaa ttcgtcgtta taatctagtt aaatcccgta
     8161 agggcgcact tatacgtcgg ataaatcgac atgtgcaaaa acgtaatcga ccttcgtcga
     8221 tttttttatt tcaaaattaa agaaacggct tgagatattc cgagcagtta taaaaattaa
     8281 tttgcgttga atattgctac tacattcttg aaaaagtgat gtagatttat agcccataac
     8341 ttttttgaat tacatcgacg atacggcgtc gaaagaaaaa taaatagacc gtatgaaaga
     8401 aaaagctacc tcactttatc tggggtagct tttatttttt atgcccttat tttgaatttt
     8461 aagagggcat tttaaagatt taggggtgat tcatatagtt ttatatctaa aacttatata
     8521 tgcttttaaa acgcaaatat gagccaaata aatatatgct attttcaaaa acaaaaattt
     8581 gagcaaaaac aataactatt tttagacact gcccatttac atgcaaatta aaaattagca
     8641 taaaattctt taaaacttcc ccacgttttt ctaaattcct gtagggcgtt tttatcaaaa
     8701 atcaagacaa gaagaaactc gttcacactc gtttcaaaaa aacaagaaat ctaatctata
     8761 cacctaccgt tttccaaccc cctcaaagca tacaaattaa aaacaaaatt attcataatc
     8821 ttactttctt ttaacaaaaa cataaaaacc ataaatagac aaattaacta tcaaaggcaa
     8881 taaacagtat aaaaaaacca cgaataaatc ttcatcaaca tctaaaataa aaaagaataa
     8941 aacaattaac aaaatactaa ttaacaaacc taaaagtggc aaacttttta tatatttttc
     9001 taaattttca ttcataaaat agactccttt caaaaatatg tttataaata tattaaaata
     9061 aatattgatt ttataaagcg cttacttata caattaaaat atcttattat cttggaggta
     9121 tcaaatgaaa attataaaat tattttcaat aatttccttg ttcctactgg tatttagcgc
     9181 gaatttttct aatgcttacg caaatgaaga acaaaaaagt tctttactag aaaatcaaaa
     9241 agaaaaattg aaaagcaaaa acatcgaagt atcacaaatg accaagaaag aaataagttt
     9301 tctagaacaa ttgcaaaata aaaatgaaga tatgcaaaaa ttcgaaaagc aaatgaaaaa
     9361 agaaggattt aaaaaaataa agctagaaga caaaaattct aaattaaaat atctagctac
     9421 agaagcaaat gataaaaatg catatggcta catagctaat aacgcttata aaaatgaaaa
     9481 aacaaaagaa attgtagttt ctgaaacaat atatgataac tacaacaaaa aaatcacaaa
     9541 attcattgcc gaaaaaagaa gtatgactga aaacaaagag ggaaaaatac ttattaacaa
     9601 aaattacgtt gaatataagg ctgaaaaagt tgataaaaac agaccggtaa catatgcagg
     9661 attcaaatgg aacggcaaag cattcgcttg tagtatggct ggactttatg catgtgctca
     9721 ctactgtgga gtatgggcaa tcgtaaatcc aatagccggc ggtgtatgtg aagcagtttg
     9781 cggaactgca tttgcagctg gatgctctgt aggttgattt tattccaatt gttttattga
     9841 cgttgagcct cggaaccctt aacaatccca aaacttgtcg aatggtcggc ttaatagctc
     9901 acgctatgcc gacattcgtc tgcaagttta gttaagggtt cttctcaaca tcaataaatt
     9961 ttttcggcat aaatacgatg ttccttattt atcctcataa aaaacaatag ctgaaatata
    10021 taaccaatta ctgtgtgttt gaatttctaa tacatgaata tcattcttta aaaacctatt
    10081 aacttgttta tctaattgac gatgagtaaa agtagcgaaa gttttaatct tcatgatatc
    10141 tctcctttta taaaaatagc aatccttatt acaatattaa acacttttat tttggatttt
    10201 aaggaggcat tttgaagact tagggcaaat catataggtt tttaacttaa aaatatatac
    10261 aagctcttaa aacgcaaata tgagccaaat aaatatatgg taatttcaca aacaaaaatt
    10321 tcagcaaaac cagtgactaa ttttttaaca ctgcccattt acatgcaaat taaaaattag
    10381 cataaaatcc tttaaaactt ccacatgttt ttctaaattc ctgcagggcg ttttttatca
    10441 aaaatcgaga atttttaatt tttctctaaa aactccgtaa gagcataagg agaatttgct
    10501 atcattcaaa aatgataggt ctggcaaagc cagaaattcc aggggtttta aggtatttta
    10561 aagtgatatc atacaaagct gtactgtcac acttttgtga cactacatac agcatttgtc
    10621 acaaaaataa gacagtactt ttttataaaa aatcaacaaa aaagacatta gtcaacacta
    10681 atgtcttaaa taataatttt gcttttcgaa attccaaatt gcggtaattg aatgtgcttt
    10741 ttcttcttcg tctgttgttt tatcttcatc acttgcatta atcaaattac catcttcgac
    10801 atcgtctaag tttaaaattt tatgtttttg tttaagcaat ccaccataac ttaacatacg
    10861 ttttcgatac aaaccttttt ctaaatcact tacaatttct tgattttttt catcatcatc
    10921 agttaaaaaa tcagatgatt taaccgaata ttttgaggtc tcttcgagtg ttataaaatc
    10981 atttgaatta ttgattaaag atattatatt gtttcatgtt tttacggcgc atataaaggg
    11041 catgtgtttc ttcaagttca gttagaattg tttgcattaa ataagcttgc attgacttga
    11101 ttgtttcctt ttggccttta actcccctat catgtatagc ttttttaagt ctttttaaag
    11161 aagttaatat ttcatcctca atgtcctcta acatgtaatg catatcttca tgccttgaat
    11221 taaatgattt ttttgcttta agaataacag cctttatgat ttctaaatct ctataatcat
    11281 attttaataa ataattagtt agttgttctg ggaattcttg caatagcatt tctttatcat
    11341 caaaattatg tgtaaattga tttgaatgat ttgaatgatt actaatttca tttttgtact
    11401 ttatatcatt caagtcattc atatcattat tctcagtatc attaaagtca atgtcattaa
    11461 agtcagtatc attagtgttc gattttcgaa cttctggacg ttcgattttc gaacttctgg
    11521 acgttcgatt ttcgaacttc tggacgttcg attttcgaac ttccttctcg ccgtaaggtt
    11581 caacatcgtt ttcttctttt tctattttgt aaatatcacg ttctgtgaca ataggtttca
    11641 ttaaatatag aatatttggt ttatttaaac cacgtcgttt ttgaatgagt agaccatcgt
    11701 tttctaaccc tttttttatt tttgttaact tttctttttt acaatttaag atttccatta
    11761 gcttttcatt tgaataaaca aagtaaatgt taccatcttc atcaacccag ctatttttaa
    11821 tagataaatt taagcgatca cgtaagatag cataggctat tttcatatca ttagttagat
    11881 ttttataatt ttcactggta aagaatactt tagggatttg ataaaatctt tctttgtaat
    11941 tttcttgcac tgtaaaatat tgattttgca tgacaaaaaa cctctttcca tattttagtt
    12001 tggtaagagg taataaaacg tttttattta gctttacatt tatttaatga tttaggtgct
    12061 tgcactaaga tattattcat tatataatac aagtatcgaa aattatataa ataaacagtt
    12121 tctaaacgtt ttattaccaa accccgacta ttaccagtag ttggggtttt tgttatatta
    12181 agtttattat agcactttaa ataaacttat aaaagggaaa atatagcttt atcaagggtt
    12241 tataaaccct tgataaatat cataaacctt tcataaaccc ttgataaaaa ggttgatgga
    12301 tgtttatttt tatttattaa tcactttatt attaagcatt ataagcttat aatattttat
    12361 caagggttta taaacatttt ataaagggtt gatgatatga aaagtgtgaa aaagttgagt
    12421 gaagaattag gggttagtaa gcaaacgatt tttaataata ttaagcgatt gaatatagag
    12481 acaataaaac aggaaaacac ttcgtttatt aaagaagata ccgatataga aaaaatcatt
    12541 caaagagtaa atgaaaataa aaagaaatat ggttttgaaa gtactactga agacaaacaa
    12601 aaaaaagggt cggataatat taattttgaa tcgaaaagcg atgctcaaat agtagaaatc
    12661 cttaaaaatc agattgatac tttaaataat caaattgaaa aacaagaaag tcgtcatgaa
    12721 acaacaattg aattttacag aaaagagttg caagaaagat caaaattgct tgaaaatcag
    12781 caagttctgg cattagagag taataaaaag attcaaaaat tagaaaatca attagaaaaa
    12841 gaaaaacacc ttaattatcc ctttgataca tctactaaca ctagacaaaa cgttgatgca
    12901 caagaaaaaa catatactac taatcctgta aatattaaca gagatcagga agaaactaaa
    12961 gaaacagaga ttcaatataa ggatatatct ggaagtcaaa gcgacgaaag tacacaaggt
    13021 gaagaagcaa aaaaagaaga cgttccagct aatctaaatg acaatgatag tgatatagaa
    13081 gaaaagagtg aagaaacgga acctaaaaag gggttttgga gtcgtttgtt tggtagctaa
    13141 aataattgat taggcactaa aa
//
