LOCUS       NZ_CP062330             3332 bp    DNA     circular CON 13-MAY-2024
DEFINITION  Staphylococcus aureus strain NAS_OP_026 plasmid pKH18, complete
            sequence.
ACCESSION   NZ_CP062330
VERSION     NZ_CP062330.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN15567978
            Assembly: GCF_022404655.1
KEYWORDS    RefSeq.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 3332)
  AUTHORS   Cella,E., Sutcliffe,C., Jubair,M., Weatherholtz,R.C., Reid,R.,
            Reid,A., Quay,D., Azarian,T.H. and Hammitt,L.
  TITLE     Carriage prevalence and genomic epidemiology of Staphylococcus
            aureus among Native Americans in the southwest US
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3332)
  AUTHORS   Cella,E., Sutcliffe,C., Jubair,M., Weatherholtz,R.C., Reid,R.,
            Reid,A., Quay,D., Azarian,T.H. and Hammitt,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JUL-2020) Burnett School of Biomedical Sciences,
            University of central Florida, 4111 Libra Dr, Orlando, FL 32816, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 2020
            Assembly Method                   :: Uniclycler v. 0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 50x
            Sequencing Technology             :: Illumina MiSeq; Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_022404655.1-RS_2024_05_13
            Annotation Date                   :: 05/13/2024 03:53:46
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,701
            CDSs (total)                      :: 2,619
            Genes (coding)                    :: 2,555
            CDSs (with protein)               :: 2,555
            Genes (RNA)                       :: 82
            rRNAs                             :: 7, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 36 of 64
            Pseudo Genes (incomplete)         :: 32 of 64
            Pseudo Genes (internal stop)      :: 14 of 64
            Pseudo Genes (multiple problems)  :: 17 of 64
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP062330.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3332
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /strain="NAS_OP_026"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /plasmid="pKH18"
                     /collected_by="UCF"
     gene            complement(687..1682)
                     /locus_tag="IF761_RS13465"
                     /old_locus_tag="IF761_13390"
     CDS             complement(687..1682)
                     /locus_tag="IF761_RS13465"
                     /old_locus_tag="IF761_13390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="WP_001199887.1"
                     /translation="MQYNTTRSITENQDNKTLKDMTKSGKQRPWREKKIDNVSYADILE
                     ILKIKKAFNVKQCGNILEFKPTDEGYLKLHKTWFCKSKLCPVCNWRRAMKNSYQAQKVI
                     EEVIKEKPKARWLFLTLSTKNAIDGDTLEQSLKHLTKAFDRLSRYKKVKQNLVGFMRST
                     EVTVNKNDGSYNQHMHVLLCVENAYFRKKENYITQEEWVNLWQRALQVDYRPVANIKAI
                     KPNKKGDKDIESAIKETSKYSVKSSDFLTDDDEKNQEIVSDLEKGLYRKRMLSYGGLLK
                     QKHKILNLDDAEDGNLINTSDEDKTTDEEEKAHSITAIWNFEKQNYYLRH"
                     /name="protein rep"
     gene            complement(1873..2220)
                     /locus_tag="IF761_RS13470"
                     /old_locus_tag="IF761_13395"
     CDS             complement(1873..2220)
                     /locus_tag="IF761_RS13470"
                     /old_locus_tag="IF761_13395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020366422.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0003700 - DNA-binding transcription factor activity
                     [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="WP_000119690.1"
                     /translation="MSYENTCDVICVHEDKVNNALSFLEDDKSKKLLNILEKICDEKKL
                     KIILSLIKEDELCVCDISLILKMSVASTSHHLRLLYKNEVLDFYKDGKMAYYFIKDDEI
                     REFFSKNHEGF"
                     /name="metalloregulator ArsR/SmtB family transcription
                     factor"
     gene            complement(2239..2856)
                     /gene="cadD"
                     /locus_tag="IF761_RS13475"
                     /old_locus_tag="IF761_13400"
     ARG             complement(2243..2856)
                     /name="cadD"
                     /gene="cadD"
                     /gene_name="cadmium resistance transporter CadD"
                     /tool="amrfinderplus"
                     /drug_class="CADMIUM"
                     /identity="100.0"
                     /coverage="100.0"
     Typing          complement(688..1682)
                     /name="rep_cluster_1017"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 aaaagaacgc gtgcaaaaac cataatcgac cttcgtcgat tttttatatt cgttttaaga
       61 aatcggcgtt gtggcgcaga tataaaaatt aatactacat caagacaaaa gctactagtt
      121 ttttgctagt agctttttta ttttgttaat ttttaaatgc ctttattttg aattttaaga
      181 ggtcatttta aagatttatg ggtaaatcat atagttttat acctaaaaac ctacataaac
      241 ttttaaaacg caaatatgag ccaaataaat atatcttaat ttcacaaact aaaattcgag
      301 taaaaccagt gtcgagtttt ttagacactg cccatttaca tgcaaattaa aaatgagcat
      361 aaaattcttt aaaacttccc catgtttttc taaattcctg cagggcgttt ttatcaaaaa
      421 tcgagaaatt tcaatttttc tctaagaact ccgtaagagc ataaggaaaa tatgacccca
      481 tctatagatg aggtatctgg caaagccaga tatttcaaga cttcgtcgat ctttttgcat
      541 gggggcataa aaagacgaaa tgtcacactt ttgtgacaca atatattgcg tttgtcacag
      601 ttttgtgaca ctacatacag aatttgtcac aaaaataaga cagcactttt ttacaaaaaa
      661 gtaataaaaa aagacatcag ttatcactaa tgccttaaat aataattttg cttttcaaaa
      721 ttccaaattg ccgtaatcga atgtgccttt tcttcttcat ctgttgtttt atcttcatca
      781 cttgtattaa tcaaatttcc atcttcagca tcgtctaagt ttaaaatttt atgcttttgt
      841 ttcagcaatc caccataact taacatacgc tttcgataca aacctttttc taaatcactt
      901 acaatttctt gatttttttc atcatcatca gttaaaaaat cagatgattt aaccgaatat
      961 tttgaggtct ctttgattgc agattcaata tctttatcgc cttttttatt tggtttgatc
     1021 gctttaatat tagcaacagg tcgataatca acttgcaatg ctctttgcca taaattaacc
     1081 cattcttctt gagttatata attctctttt tttctaaaat atgcattttc aacacataac
     1141 aaaacgtgca tatgctgatt ataactaccg tcatttttat taacggtaac ttctgttgaa
     1201 cgcataaatc caacaagatt ttgtttaacc tttttatatc tactcaacct atcaaatgct
     1261 ttagttagat gcttcaaact ttgttctaaa gtatctccat ctatcgcatt ttttgttgaa
     1321 agtgttaaaa acaaccaacg tgcttttggc ttttccttaa ttacttcttc aatcactttt
     1381 tgagcttgat aactattttt catagcacgt ctccaattac aaaccggaca taattttgat
     1441 ttacaaaacc atgtcttatg taacttcaaa taaccttcat cagttggctt aaattctaaa
     1501 atattaccac attgttttac attaaaagcc tttttgattt ttaaaatttc tagtatatct
     1561 gcatagctta cattatctat tttcttttct ctccatgggc gttgtttccc acttttcgtc
     1621 atatctttta acgttttatt atcttgattt tcggttatac ttctagtagt attatattgc
     1681 atataaaaag acccccagta ttatttaaga ttcgacacct taataatata actggggttt
     1741 tttattgtca aattttcctt tcacgcacaa aaacacgttt aaacgctgta atatcaacag
     1801 ttacagctgt atatataaaa tcgagttatt tctatatagt atcaagacaa gaagaaactc
     1861 gtttcactcg tttcaaaaac cctcatgatt tttagagaaa aactctctta tttcatcgtc
     1921 tttaataaaa taatatgcca tctttccatc tttataaaaa tcaagtacct catttttata
     1981 taaaagcctt aaatgatgtg aagttgaagc aacactcatt ttcaatatca aagaaatatc
     2041 acaaacacac aactcatctt ctttaatcaa agataatatg attttcaatt tcttctcatc
     2101 acaaattttt tctaaaatgt taagtaattt cttagattta tcatcttcta aaaaacttaa
     2161 agcattgtta actttatcct catgtacaca gatcacatca caagtatttt cataactcat
     2221 ataaaatcac accctaaatt aaaatataaa tcctaaaatt gtttgaatag tgtcattttc
     2281 aataataata aataaaccta aagctatata aatgatagcc ataatccaac gactaaattt
     2341 ctcaacaatt tctccaattc ctgggatatt agctaatttt tgtgcagtaa acaccaagaa
     2401 gaaaattaaa attaaaaaga caaacagagt aagtaataaa ttagtaacac ttaatgtcac
     2461 aaaatacgga acaaataaac caatattatc ggcgccacaa cttgctatcg taacaattgc
     2521 aaccgtacca actaatttag acaatccttt ttcattcaat tcttttttag ctctcttttc
     2581 tccttcacaa tcatcataaa tagccacttt aattccaaga taaattggta ttaaacccaa
     2641 taaacctaat atccactttt ctggaacata atttaataca aaagctaaaa acaaactaac
     2701 taatattaaa ataatagacc ctaaatattg accaacataa atatctctat attcttttct
     2761 agtctttgct ctagcaaaaa atattaataa aattactaat aaatcaactg ctgtagcaat
     2821 ataaagaaca gcagcagcaa caaccgtttg aatcataata cacctcattc aaaaatattt
     2881 ttgaatgtat tataacttat atttttaaat aatcataggt gctttattcc aattgcttta
     2941 ttgacgttga gcctcggaac ccttaacaat cccaaaactt gtcgaatggt cggcttaata
     3001 gctcacgcta tgccgacatt cgtctgcaag tttagttaag ggttcttctc aacgcacaat
     3061 aaattttctc ggcataaatg cgtgtctatg cgtagacatg gagacacttc agaattatgg
     3121 agtgattaca tgagtttaac tatttttctt tattcagttt taggaagttt attagcaaat
     3181 tatatctacg aaaaatcttc aaaataaata actttttcgg ttctactttg tacactttat
     3241 ctacctggta gacctaattg ttattgcttt acaggtctgt ttttcgtcgt tataatctag
     3301 ttaaatcccg taagggcgca cttatgcgtt ct
//
