LOCUS       NZ_CP062315            27035 bp    DNA     circular CON 12-MAY-2024
DEFINITION  Staphylococcus aureus strain NAS_OP_133 plasmid unnamed1, complete
            sequence.
ACCESSION   NZ_CP062315
VERSION     NZ_CP062315.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN15567993
            Assembly: GCF_022369595.1
KEYWORDS    RefSeq.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 27035)
  AUTHORS   Cella,E., Sutcliffe,C., Jubair,M., Weatherholtz,R.C., Reid,R.,
            Reid,A., Quay,D., Azarian,T.H. and Hammitt,L.
  TITLE     Carriage prevalence and genomic epidemiology of Staphylococcus
            aureus among Native Americans in the southwest US
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 27035)
  AUTHORS   Cella,E., Sutcliffe,C., Jubair,M., Weatherholtz,R.C., Reid,R.,
            Reid,A., Quay,D., Azarian,T.H. and Hammitt,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JUL-2020) Burnett School of Biomedical Sciences,
            University of central Florida, 4111 Libra Dr, Orlando, FL 32816, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 2020
            Assembly Method                   :: Uniclycler v. 0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 50x
            Sequencing Technology             :: Illumina MiSeq; Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_022369595.1-RS_2024_05_12
            Annotation Date                   :: 05/12/2024 03:55:40
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,829
            Genes (coding)                    :: 2,745
            CDSs (with protein)               :: 2,745
            Genes (RNA)                       :: 82
            rRNAs                             :: 7, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 45 of 84
            Pseudo Genes (incomplete)         :: 41 of 84
            Pseudo Genes (internal stop)      :: 18 of 84
            Pseudo Genes (multiple problems)  :: 19 of 84
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP062315.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..27035
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /strain="NAS_OP_133"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /plasmid="unnamed1"
                     /collected_by="UCF"
     gene            complement(join(26991..27035,1..750))
                     /gene="aph(3')-IIIa"
                     /locus_tag="IF776_RS14350"
                     /old_locus_tag="IF776_14335"
     ARG             complement(2..750)
                     /name="aph(3')-IIIa"
                     /gene="aph(3')-IIIa"
                     /gene_name="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /tool="amrfinderplus"
                     /drug_class="AMINOGLYCOSIDE"
                     /identity="100.0"
                     /coverage="94.7"
     gene            complement(843..1373)
                     /gene="sat4"
                     /locus_tag="IF776_RS14355"
                     /old_locus_tag="IF776_14340"
     ARG             complement(847..1373)
                     /name="sat4"
                     /gene="sat4"
                     /gene_name="streptothricin N-acetyltransferase Sat4"
                     /tool="amrfinderplus"
                     /drug_class="STREPTOTHRICIN"
                     /identity="97.78"
                     /coverage="100.0"
     gene            complement(1370..>2005)
                     /locus_tag="IF776_RS14360"
                     /old_locus_tag="IF776_14345"
                     /pseudo=""
     CDS             complement(1370..>2005)
                     /locus_tag="IF776_RS14360"
                     /old_locus_tag="IF776_14345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009251744.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside 6-adenylyltransferase"
                     /name="aminoglycoside 6-adenylyltransferase"
     gene            complement(2146..2820)
                     /locus_tag="IF776_RS14365"
                     /old_locus_tag="IF776_14350"
     CDS             complement(2146..2820)
                     /locus_tag="IF776_RS14365"
                     /old_locus_tag="IF776_14350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105942.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS257 family transposase"
                     /protein_id="WP_001105956.1"
                     /translation="MNYFRYKQFNKDVITVAVGYYLRYALSYRDISEILRERGVNVHHS
                     TIYRWVQEYAPILYQIWKKKHKKAYYKWRIDETYIKIKGKWSYLYRAIDTEGHTLDIWL
                     RKQRDNHSAYAFIKRLIKQFGKPQKVITDQAPSTKVAMVKVIKAFKLKPDCHCTSKYLN
                     NLIEQDHRHIKVRKTRYQSINTAKNTLKGIECIYALYKKNRRSLQIYGFSPCHEISIML
                     AS"
                     /name="IS6-like element IS257 family transposase"
     gene            complement(<2877..3053)
                     /locus_tag="IF776_RS14370"
                     /old_locus_tag="IF776_14355"
                     /pseudo=""
     CDS             complement(<2877..3053)
                     /locus_tag="IF776_RS14370"
                     /old_locus_tag="IF776_14355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499250.1"
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_function="GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA]"
                     /GO_process="GO:0016311 - dephosphorylation [Evidence IEA]"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /name="undecaprenyl-diphosphate phosphatase"
     gene            complement(3067..3804)
                     /locus_tag="IF776_RS14375"
                     /old_locus_tag="IF776_14360"
     CDS             complement(3067..3804)
                     /locus_tag="IF776_RS14375"
                     /old_locus_tag="IF776_14360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000369394.1"
                     /GO_function="GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]; GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="WP_000369394.1"
                     /translation="MDNLLRTEFIKLKRSYMFLISILGTCVAPFMVVVASFISMRTKVP
                     TPNIVFKQLFEEVSLYHMLIIGPPLYGVIIAYLFGREYIEDTLKNLLIIPISRESFLVS
                     KIILLFLWIQTLSVIAWLLTLMLGMLLHFQGLSQSLIIDSFFTFVIGGVLLWILSPPII
                     ILSIYFKNYVPTIVLTIIITLINLMTANSEHRGLFPWAAVGDISRGTLPNTYPFLLSLI
                     IIIVTSIFSVIILFSYFKKTDIS"
                     /name="ABC transporter permease"
     gene            complement(3797..4726)
                     /locus_tag="IF776_RS14380"
                     /old_locus_tag="IF776_14365"
     CDS             complement(3797..4726)
                     /locus_tag="IF776_RS14380"
                     /old_locus_tag="IF776_14365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000074567.1"
                     /GO_function="GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]; GO:0005524 - ATP binding [Evidence IEA];
                     GO:0016887 - ATP hydrolysis activity [Evidence IEA];
                     GO:0042626 - ATPase-coupled transmembrane transporter
                     activity [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="WP_000793446.1"
                     /translation="MKNLNNIIEINNLTKQYGSQIAVNNLSITVESGQIYGFLGRNGAG
                     KTTTIRMLLGLIKPTKGNIKIFEKEFESNKTEILKNIGAMVETPGFYSNLTAKENLTIN
                     AKILGIQKENAIEEVLEIVGLASEKVKLFKNYSLGMKQRLGIARALLHNPELLILDEPT
                     NGLDPIGIKEIRKLIKDLAQSRNITILISSHILSEIEQLVDKVGIIHNGVLLEEIMYED
                     LKKKNRQFISISVSDEEKVACLLETNFGIYDYKIKENGEFKIYSHIDRQQEINRLLVLN
                     NIDVFQMRVIEDNLETYFEDLVGGGTIG"
                     /name="ABC transporter ATP-binding protein"
     gene            complement(4842..5723)
                     /locus_tag="IF776_RS14385"
                     /old_locus_tag="IF776_14370"
                     /pseudo=""
     CDS             complement(4842..5723)
                     /locus_tag="IF776_RS14385"
                     /old_locus_tag="IF776_14370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015581923.1"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA];
                     GO:0000155 - phosphorelay sensor kinase activity [Evidence
                     IEA]; GO:0004673 - protein histidine kinase activity
                     [Evidence IEA]"
                     /GO_process="GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /name="sensor histidine kinase"
     gene            complement(5720..>6097)
                     /locus_tag="IF776_RS14390"
                     /old_locus_tag="IF776_14375"
                     /pseudo=""
     CDS             complement(5720..>6097)
                     /locus_tag="IF776_RS14390"
                     /old_locus_tag="IF776_14375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019834602.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]; GO:0006355 - regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /name="response regulator transcription factor"
     gene            complement(6155..6829)
                     /locus_tag="IF776_RS14395"
                     /old_locus_tag="IF776_14380"
     CDS             complement(6155..6829)
                     /locus_tag="IF776_RS14395"
                     /old_locus_tag="IF776_14380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105942.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="WP_001105960.1"
                     /translation="MNYFRYKQFNKDVITVAVGYYLRYALSYRDISEILRERGVNVHHS
                     TIYRWVQEYAPILYQIWKKKHKKAYYKWRIDETYIKIKGKWSYLYRAIDTEGHTLDIWL
                     RKQRDNHSAYAFIKRLIKQFGKPQKVVTDQAPSTKAAMAKIIKAFKLKPDCHCTSKYLN
                     NLIEQDHRHIKVRKTRYQSINTAKNTLKGIECIYALYKKNRRSLQIYGFSPCHEISIML
                     AS"
                     /name="IS6 family transposase"
     gene            7303..8769
                     /gene="msr(A)"
                     /locus_tag="IF776_RS14400"
                     /old_locus_tag="IF776_14385"
     ARG             7304..8766
                     /name="msr(A)"
                     /gene="msr(A)"
                     /gene_name="ABC-F type ribosomal protection protein Msr(A)"
                     /tool="amrfinderplus"
                     /drug_class="MACROLIDE/STREPTOGRAMIN"
                     /identity="100.0"
                     /coverage="100.0"
     gene            8868..9767
                     /locus_tag="IF776_RS14405"
                     /old_locus_tag="IF776_14390"
     CDS             8868..9767
                     /locus_tag="IF776_RS14405"
                     /old_locus_tag="IF776_14390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000196697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mph(C) family macrolide 2'-phosphotransferase"
                     /protein_id="WP_000196697.1"
                     /translation="MTRHNEIIKCAEKYQLHIQPQTISLNESGLDFQVAFGKDKHGVEW
                     VLRLPRRPDVYKRTKPEKQTVDFLQKNVSFEIPKWKVHAKDLIAYPKLTGKPAATIDPE
                     IQNYVWEIEHKPLPENFINTLAETLVDLHNIPEENINVQHINIKTIQEIKNDFQRRMNK
                     VKETYGVSDELWNRWKQWLENDELWPRHATMIHGDLHPGHIMVDNQANVTGLIDWTEAT
                     HSDPSMDFIGHHRVFDDEGLEQLITAYGKAGGEIWPRMKEHIIELNAVFPMFIAEFAME
                     SGESAYETMALKELGMKE"
                     /name="Mph(C) family macrolide 2'-phosphotransferase"
     gene            10253..10861
                     /locus_tag="IF776_RS14410"
                     /old_locus_tag="IF776_14395"
     CDS             10253..10861
                     /locus_tag="IF776_RS14410"
                     /old_locus_tag="IF776_14395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002474535.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0000150 - DNA strand exchange activity [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="WP_000583528.1"
                     /translation="MIIGYARVSSIDQNLERQLDNLKTFGVEKIFTEKQSGKSVENRPV
                     FQEALNFVRMGDRFVVESIDRLGRNYDEIIETVNYLKEKDVQLMITSLPMMNEVIGNPL
                     LDKFMKDLIIQILAMVSEQERNESKRRQAQGIQVAKEKGVYKGRPLLYSPNAKDPQKRV
                     IYHRVVEMLEEGKAISKIAKEVNITRQTVYRIKHDKGLS"
                     /name="recombinase family protein"
     gene            11107..11445
                     /locus_tag="IF776_RS14415"
                     /old_locus_tag="IF776_14400"
     CDS             11107..11445
                     /locus_tag="IF776_RS14415"
                     /old_locus_tag="IF776_14400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001049146.1"
                     /translation="MNLIPFIIILIFSCLWTAITNKYLPSETLDKKGQQDRYDERQRKM
                     FIEILAKSFIWIVYCMLFTLVLKFTGLSPSIEGSWFSQYPEIFFIIVALFLMLFNYYTT
                     NKKYTSKG"
                     /name="hypothetical protein"
     gene            11450..11635
                     /locus_tag="IF776_RS14420"
                     /old_locus_tag="IF776_14405"
     CDS             11450..11635
                     /locus_tag="IF776_RS14420"
                     /old_locus_tag="IF776_14405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011276570.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="WP_000797248.1"
                     /translation="MKNRIREYRKQKGISQETLSKELKVSRQTINAIENNKYDPTLTLA
                     FKLARLFDTTVDELFS"
                     /name="helix-turn-helix transcriptional regulator"
     gene            complement(11862..12605)
                     /locus_tag="IF776_RS14425"
                     /old_locus_tag="IF776_14410"
     CDS             complement(11862..12605)
                     /locus_tag="IF776_RS14425"
                     /old_locus_tag="IF776_14410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000843735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="WP_000843732.1"
                     /translation="MKSVKKLSEELGVSKQTIFNNIKRLNIETIKQENTSFIKEDTDIE
                     KIIQRVNENKKKYGFESTTEDKQKKGSDNINFESKSDAQIVEILKNQIDTLNNQIEKQE
                     SRHETTIEFYRKELQERSKLLENQQVLALESNKKIQKLENQLEKEKHLNYPFDTSTNTR
                     QNVDAQEKTYTTNPVNINRDQEETKETEIQYKDISGSQSDESTQGEEAKKEDVPANLND
                     NDSDIEEKSEETEPKKGFWSRLFGS"
                     /name="DUF536 domain-containing protein"
     gene            13000..13983
                     /locus_tag="IF776_RS14430"
                     /old_locus_tag="IF776_14415"
     CDS             13000..13983
                     /locus_tag="IF776_RS14430"
                     /old_locus_tag="IF776_14415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001181101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="WP_001181107.1"
                     /translation="MQNQYFTVQENYKERFYQIPKVFFTSENYKNLTNDMKIAYAILRD
                     RLNLSIKNSWVDEDGNIYFVYSNEKLMEILNCKKEKLTKIKKGLENDGLLIQKRRGLNK
                     PNILYLMKPIVTERDIYKIEKEENDVEPYGEKEVRKSNVQKFENRTSRSSKIERPEVRK
                     SNTNDTDFNDIDFNDTENNDMNDLNDIKYKNEISNHSNHSNQFTHNFDDKEMLLQEFPE
                     QLTNYLLKYDYRDLEIIKAVILKAKKSFNSRHEDMHYMLEDIEDEILTSLKRLKKAIHD
                     RGVKGQKETIKSMQAYLMQTILTELEETHALYMRRKNMKQYNIFNQ"
                     /name="replication initiator protein A"
     gene            <14003..14293
                     /locus_tag="IF776_RS14435"
                     /old_locus_tag="IF776_14420"
                     /pseudo=""
     CDS             <14003..14293
                     /locus_tag="IF776_RS14435"
                     /old_locus_tag="IF776_14420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009384749.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006260 - DNA replication [Evidence IEA]"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /name="protein rep"
     gene            14837..14986
                     /locus_tag="IF776_RS14440"
                     /old_locus_tag="IF776_14425"
     CDS             14837..14986
                     /locus_tag="IF776_RS14440"
                     /old_locus_tag="IF776_14425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000695367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000695366.1"
                     /translation="MKIKTFATFTHRQLDKQVNRFLKNDIHVLEIQTHSNWLYISAIVF
                     YEDK"
                     /name="hypothetical protein"
     gene            complement(15154..15846)
                     /locus_tag="IF776_RS14445"
                     /old_locus_tag="IF776_14430"
     CDS             complement(15154..15846)
                     /locus_tag="IF776_RS14445"
                     /old_locus_tag="IF776_14430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000691527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative immunity/bacteriocin fusion bifunctional
                     protein"
                     /protein_id="WP_000691527.1"
                     /translation="MKIIKLFSIISLFLLVFSANFSNAYANEEQKSSLLENQKEKLKSK
                     NIEVSQMTKKEISFLEQLQNKNEDMQKFEKQMKKEGFKKIKLEDKNSKLKYLATEANDK
                     NAYGYIANNAYKNEKTKEIVVSETIYDNYNKKITKFIAEKRSMTENKEGKILINKNYVE
                     YKAEKVDKNRPVTYAGFKWNGKAFACSMAGLYACAHYCGVWAIVNPIAGGVCEAVCGTA
                     FAAGCSVG"
                     /name="putative immunity/bacteriocin fusion bifunctional
                     protein"
     gene            15956..16150
                     /locus_tag="IF776_RS14450"
                     /old_locus_tag="IF776_14435"
     CDS             15956..16150
                     /locus_tag="IF776_RS14450"
                     /old_locus_tag="IF776_14435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001004925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001004925.1"
                     /translation="MNENLEKYIKSLPLLGLLISILLIVLFFFILDVDEDLFVVFLYCL
                     LPLIVNLSIYGFYVFVKRK"
                     /name="hypothetical protein"
     gene            17266..17883
                     /gene="cadD"
                     /locus_tag="IF776_RS14455"
                     /old_locus_tag="IF776_14440"
     ARG             17267..17880
                     /name="cadD"
                     /gene="cadD"
                     /gene_name="cadmium resistance transporter CadD"
                     /tool="amrfinderplus"
                     /drug_class="CADMIUM"
                     /identity="100.0"
                     /coverage="100.0"
     gene            17902..18249
                     /locus_tag="IF776_RS14460"
                     /old_locus_tag="IF776_14445"
     CDS             17902..18249
                     /locus_tag="IF776_RS14460"
                     /old_locus_tag="IF776_14445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020366422.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0003700 - DNA-binding transcription factor activity
                     [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="WP_000119690.1"
                     /translation="MSYENTCDVICVHEDKVNNALSFLEDDKSKKLLNILEKICDEKKL
                     KIILSLIKEDELCVCDISLILKMSVASTSHHLRLLYKNEVLDFYKDGKMAYYFIKDDEI
                     REFFSKNHEGF"
                     /name="metalloregulator ArsR/SmtB family transcription
                     factor"
     gene            18406..18498
                     /locus_tag="IF776_RS14465"
                     /old_locus_tag="IF776_14450"
     CDS             18406..18498
                     /locus_tag="IF776_RS14465"
                     /old_locus_tag="IF776_14450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002495832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="WP_001791613.1"
                     /translation="MLIIFVHIIAPVISGCAVAYYTYWLSKRNK"
                     /name="type I toxin-antitoxin system Fst family toxin"
     gene            complement(18670..19641)
                     /locus_tag="IF776_RS14470"
                     /old_locus_tag="IF776_14455"
     CDS             complement(18670..19641)
                     /locus_tag="IF776_RS14470"
                     /old_locus_tag="IF776_14455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000481285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="WP_000481279.1"
                     /translation="MFNAVVVRKSASNVSYKVEKVDESHLSKGDVLVKVVYSSINYKDM
                     LALQYKGGVIRDYPMIPGIDFAGIVESSSNDKFKEGDNVLVTGYDVGVTHTGGFSEYAQ
                     VPGEWIVPIPQEMSIKESMVYGTAGFTAALSIFELEKYGMNIKSQPEILVSGATGGVGS
                     VAIQILHKIGYENITAIVRKEKQIEIAKKLGAKQVIIIEDDNKPLKKGIFDYFLDTVGS
                     NVTLYGLKRLNYQGVATVCGNVAGNSLNANILPFILRGIKLIGIDSVYVDHNIREDIWS
                     KLANEWNIGNSLLYNEIGFEEFYKTIDQLLKGQHLGRTILKV"
                     /name="oxidoreductase"
     gene            19765..20184
                     /locus_tag="IF776_RS14475"
                     /old_locus_tag="IF776_14460"
     CDS             19765..20184
                     /locus_tag="IF776_RS14475"
                     /old_locus_tag="IF776_14460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000418034.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0003700 - DNA-binding transcription factor activity
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="WP_000418035.1"
                     /translation="MELDNCINYLLSTSQNRVFKHFKSLLSPYNITPAEYGVLNCLINS
                     NLNTPKAIGNTLNLEPSTISGILDKMSKKDLITRQTDNENRRTVLINSTNKAKDLWPTL
                     EKKAYQLNNHYFANLTNEETVVFKKILLKINKTTF"
                     /name="MarR family transcriptional regulator"
     gene            complement(20710..21555)
                     /gene="blaZ"
                     /locus_tag="IF776_RS14480"
                     /old_locus_tag="IF776_14465"
     ARG             complement(20714..21555)
                     /name="blaZ"
                     /gene="blaZ"
                     /gene_name="penicillin-hydrolyzing class A beta-lactamase
                     BlaZ"
                     /tool="amrfinderplus"
                     /drug_class="BETA-LACTAM"
                     /identity="100.0"
                     /coverage="100.0"
     gene            21662..23419
                     /gene="blaR1"
                     /locus_tag="IF776_RS14485"
                     /old_locus_tag="IF776_14470"
     ARG             21663..23416
                     /name="blaR1"
                     /gene="blaR1"
                     /gene_name="beta-lactam sensor/signal transducer BlaR1"
                     /tool="amrfinderplus"
                     /drug_class="BETA-LACTAM"
                     /identity="95.38"
                     /coverage="100.0"
     gene            23409..23789
                     /gene="blaI"
                     /locus_tag="IF776_RS14490"
                     /old_locus_tag="IF776_14475"
     ARG             23410..23786
                     /name="blaI"
                     /gene="blaI"
                     /gene_name="penicillinase repressor BlaI"
                     /tool="amrfinderplus"
                     /drug_class="BETA-LACTAM"
                     /identity="98.41"
                     /coverage="100.0"
     gene            24053..24631
                     /locus_tag="IF776_RS14495"
                     /old_locus_tag="IF776_14480"
     CDS             24053..24631
                     /locus_tag="IF776_RS14495"
                     /old_locus_tag="IF776_14480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000690624.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0000150 - DNA strand exchange activity [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="WP_000690637.1"
                     /translation="MKIGYARVSTGLQNLNLQEDRLNTYGCEKIFNDHMSGSKSKRPGL
                     DKAIEFARSGDTIVVWRLDRLGRNMEDLITLVNELNERGVSFHSLEENITMDKSSSTGQ
                     LLFHLFAAFAEFERNLILERSSAGRIAARARGRYGGRPEKLNKQDLNLLKTLYDNGTPI
                     KTIAEQWQVSRTTIYRYLNKLEAKENKNK"
                     /name="recombinase family protein"
     gene            complement(24854..25039)
                     /locus_tag="IF776_RS14500"
                     /old_locus_tag="IF776_14485"
     CDS             complement(24854..25039)
                     /locus_tag="IF776_RS14500"
                     /old_locus_tag="IF776_14485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001037130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001037130.1"
                     /translation="MNKLEKWSLVFIIFTFLSSLALLYFKYNFSSVMPVLSLSILTILI
                     LLTSIITVVSVNDEDT"
                     /name="hypothetical protein"
     gene            25301..25975
                     /locus_tag="IF776_RS14505"
                     /old_locus_tag="IF776_14490"
     CDS             25301..25975
                     /locus_tag="IF776_RS14505"
                     /old_locus_tag="IF776_14490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105942.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS257 family transposase"
                     /protein_id="WP_001105991.1"
                     /translation="MNYFRYKQFNKDVITVAVGYYLRYALSYRDISEILRERGVNVHHS
                     TVYRWVQEYAPILYQIWKKKHKKAYYKWRIDETYIKIKGKWSYLYRAIDAEGHTLDIWL
                     RKQRDNHSAYAFIKRLIKQFGKPQKVITDQAPSTKVAMVKVIKAFKLKPDCHCTSKYLN
                     NLIEQDHRHIKVRKTRYQSINTAKNTLKGIECIYALYKKNRRSLQIYGFSPCHEISIML
                     AS"
                     /name="IS6-like element IS257 family transposase"
     gene            complement(26144..26715)
                     /locus_tag="IF776_RS14510"
                     /old_locus_tag="IF776_14495"
                     /pseudo=""
     CDS             complement(26144..26715)
                     /locus_tag="IF776_RS14510"
                     /old_locus_tag="IF776_14495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079940.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /name="HTH domain-containing protein"
     ARG             8869..9764
                     /name="mph(C)"
                     /gene="mph(C)"
                     /gene_name="Mph(C) family macrolide 2'-phosphotransferase"
                     /tool="amrfinderplus"
                     /drug_class="MACROLIDE"
                     /identity="100.0"
                     /coverage="100.0"
     ARG             7304..8769
                     /name="msrA"
                     /gene="msrA"
                     /gene_name="msr-type ABC-F protein"
                     /tool="rgi"
                     /drug_class="macrolide antibiotic; streptogramin
                     antibiotic; streptogramin B antibiotic"
                     /identity="98.36"
                     /coverage="100.0"
     ARG             8869..9767
                     /name="mphC"
                     /gene="mphC"
                     /gene_name="macrolide phosphotransferase (MPH)"
                     /tool="rgi"
                     /drug_class="macrolide antibiotic"
                     /identity="98.66"
                     /coverage="100.0"
     ARG             complement(844..1361)
                     /name="SAT-4"
                     /gene="SAT-4"
                     /gene_name="streptothricin acetyltransferase (SAT)"
                     /tool="rgi"
                     /drug_class="nucleoside antibiotic"
                     /identity="97.16"
                     /coverage="95.56"
     ARG             complement(20711..21555)
                     /name="PC1 beta-lactamase (blaZ)"
                     /gene="PC1 beta-lactamase (blaZ)"
                     /gene_name="BlaZ beta-lactamase"
                     /tool="rgi"
                     /drug_class="penam"
                     /identity="96.8"
                     /coverage="100.0"
     ARG             complement(1668..1969)
                     /name="aad(6)"
                     /gene="aad(6)"
                     /gene_name="ANT(6)"
                     /tool="rgi"
                     /drug_class="aminoglycoside antibiotic"
                     /identity="99.0"
                     /coverage="36.23"
     Typing          14349..14496
                     /name="MOBV"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="95.973"
                     /coverage="100.0"
                     /evalue="6.06e-62"
     Typing          complement(11863..12605)
                     /name="rep_cluster_1733"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          13001..13983
                     /name="rep_cluster_2100"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="99.593"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          14115..14285
                     /name="rep_cluster_1017"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.186"
                     /coverage="91.0"
                     /evalue="6.2e-68"
ORIGIN
        1 atcaggcttg atccccagta agtcaaaaaa tagctcgaca tactgttctt ccccgatatc
       61 ctccctgatc gaccggacgc agaaggcaat gtcataccac ttgtccgccc tgccgcttct
      121 cccaagatca ataaagccac ttactttgcc atctttcaca aagatgttgc tgtctcccag
      181 gtcgccgtgg gaaaagacaa gttcctcttc gggcttttcc gtctttaaaa aatcatacag
      241 ctcgcgcgga tctttaaatg gagtgtcttc ttcccagttt tcgcaatcca catcggccag
      301 atcgttattc agtaagtaat ccaattcggc taagcggctg tctaagctat tcgtataggg
      361 acaatccgat atgtcgatgg agtgaaagag cctgatgcac tccgcataca gctcgataat
      421 cttttcaggg ctttgttcat cttcatactc ttccgagcaa aggacgccat cggcctcact
      481 catgagcaga ttgctccagc catcatgccg ttcaaagtgc aggacctttg gaacaggcag
      541 ctttccttcc agccatagca tcatgtcctt ttcccgttcc acatcatagg tggtcccttt
      601 ataccggctg tccgtcattt ttaaatatag gttttcattt tctcccacca gcttatatac
      661 cttagcagga gacattcctt ccgtatcttt tacgcagcgg tatttttcga tcagtttttt
      721 caattccggt gatattctca ttttagccat ttattatttc cttcctcttt tctacagtat
      781 ttaaagatac cccaagaagc taattataac aagacgaact ccaattcact gttccttgca
      841 ttctaaaacc ttaaatacca gaaaacagct ttttcaaagt tggcgtataa catagtatcg
      901 acggagccga ttttgaaacc acaattatga tagaatttac aagctataag gttattgtcc
      961 tgggtttcaa gcattagtcc atgcaagttt ttatgctttg cccattctat agatatattg
     1021 ataagcgcgc tgcctatgcc ttgccccctg aaatccttac atacggcgat atcttctata
     1081 taagcgtacc ggttccaatt ttttcgcagt ttaacttttc cgacgcattt atcgtcttgg
     1141 tagtaaagat atattatctt atcagtattg tcaatatatt caaggcaatc tgcctcctca
     1201 tcctcttcat cctcttcgtc ttggtagctt tttaaatatg gcgcttcata gagtaattct
     1261 gtaaaggtcc aattctcgtt ttcatacctc ggtataatct tacctatcac ctcaaatggt
     1321 tcgctgggtt tatcgatatc tttcaggtgc cctgctttca tttctgtaat cactgttccc
     1381 gcctctcttc tatatcagcg gcatatgtgc tatccaggca gccggtttta ccagtgtatt
     1441 ttttatacat gtccctggta tattttgtta tattcctatc atactccgga taggcataat
     1501 gaagcctttc cgccacctca ccggataccg ccctgaacaa ttgatggcat agaaataatg
     1561 cttcccatat gttttcatag gaatccatcc ggtaggtgga caaaagtttc tcccacaaat
     1621 cctcggatat atacctttca ataaacttat agttcttgcc tacacttcat ttaaagcctg
     1681 tttcgatgcc gaccttccat gatatcattc tcagcagctc atggcgaaca atctgattaa
     1741 aatgatcaat agcaaataaa atttccttac ggcacaatcc tttaataaca taaggtgtta
     1801 cattccaaaa ttcattgcag caatcatcat actcccttgc gcttggcttt cttacatgat
     1861 aatctatatc agtcggaact atgtcccttt taattctaca atctttatca attagaacct
     1921 ttattaattt atcgcccttt aggtaattat ctaactcttc caagggcaat aaggtaagat
     1981 caattttatt gtaatcatca aatagctata atagatttag aggtaaagaa attaaataca
     2041 ttgaagagca gaaagatttt acaaaagagt attttaataa aaatgatggt tctgttgcaa
     2101 agtaaaaaat atagctaatc actaatatat catgtcagtg ttcgtttaac ttgctagcat
     2161 gatgctaatt tcgtggcatg gcgaaaatcc gtagatctga agagacctgc ggttcttttt
     2221 atatagagcg taaatacact caataccttt taaagtattc tttgctgtat tgatactttg
     2281 ataccttgtc tttcttactt taatatgacg gtgatcttgc tcaatgaggt tattcagata
     2341 tttcgatgta caatggcagt caggtttaag tttaaaagct ttaattactt taaccattgc
     2401 taccttcgtt gaaggtgcct gatctgtaat taccttttga ggtttaccaa attgtttaat
     2461 gagacgtttg ataaacgcat atgctgaatg attatctcgt tgcttacgca accaaatatc
     2521 taatgtatgt ccctctgtat caatggcacg atataaatag ctccattttc cttttatttt
     2581 gatatacgtc tcatcaatac gccatttgta ataagctttt ttatgctttt tcttccaaat
     2641 ttgatataaa attggggcat attcttgaac ccaacggtag atcgttgaat gatgaacgtt
     2701 tacaccacgt tctcttaata tttcagatat atcacgatag ctcaatgcat atcttagata
     2761 gtagccaacg gctacagtga taacatcctt gttaaattgt ttatatctga aatagttcat
     2821 acagaagact cctttttgtt aaaattatat tataaattca actttgcaac agaacctcct
     2881 aactgtatta ccaccttaaa agtagttgca gacgcacttc ctaaatcgtc agcagtgttt
     2941 aaccacatat catcaacaat aatcatatga cctgttgatg atactggagc aaattctgtc
     3001 aacccttcaa ctacacctaa aataaatgct ttaatctgct ctataaaacc catttttctc
     3061 ctccttttaa cttatatcag tttttttaaa gtatgagaat aatataatta cactgaatat
     3121 agatgtaaca attatgataa ttaaagataa tagaaatgga taagtattag gtaatgttcc
     3181 tctagatatg tctccaactg cagcccaagg aaataatcct ctatgttcag aattagcagt
     3241 cattaaattt atcaaagtaa ttattatggt taaaacaatt gtgggaacat aatttttaaa
     3301 atatatcgac aaaataatta ttggtggaga taatatccat aataacactc cgccaataac
     3361 aaatgtaaag aatgaatcaa ttattaaact ctggcttaac ccttgaaaat gcaatagcat
     3421 acctagcatt aaagttagca accatgcaat aacagatagt gtttgaatcc acaagaataa
     3481 taatattatt ttacttacta aaaaactttc tctagatata ggtataatta ataagttttt
     3541 taaagtatct tctatatatt ctcttccaaa caaataggct attataaccc catatagagg
     3601 aggtccaata attagcatat gatataaact gacttcttca aataactgtt taaatacaat
     3661 attaggagtc ggtactttcg tccgcatact aataaaagaa gctacaacca ccataaaagg
     3721 tgctacgcat gtacctaaaa tacttattag aaacatatat gatcttttta acttaataaa
     3781 ttctgtacgg agtagattat ccaattgtgc cacctcctac taagtcttcg aaataagttt
     3841 ctagattatc ttctatcact ctcatctgga aaacatcaat attgtttaat actaataatc
     3901 tattaatttc ttgttgtcta tcaatatgtg aataaatttt aaattcgcca ttttctttaa
     3961 ttttatagtc ataaattccg aagttagttt ccaacaaaca tgcaactttt tcttcatctg
     4021 atactgatat acttataaat tgtctgttct ttttttttaa atcttcatac ataatttctt
     4081 ctaacaaaac tccgttatga ataataccca ccttgtctac taactgctct atttctgaca
     4141 atatatgact tgaaattaaa atggttatat ttctagattg agccagatcc ttaatcaact
     4201 ttctaatttc ttttatgcca ataggatcta atccattcgt cggctcatca agtatcaata
     4261 attcgggatt gtgcaataaa gctctcgcaa tccctaaacg ttgcttcata cctaaagaat
     4321 aatttttaaa caattttact ttttcagaag ctagtcctac tatttctaac acttcttcaa
     4381 ttgcattttc tttttgtata cctaatattt tagcatttat agttaagttc tctttagctg
     4441 tcaaattact ataaaaacct ggcgtttcaa ccatagcacc aatatttttt aaaatttcag
     4501 ttttattact ttcgaactct ttttcaaata ttttaatatt acctttcgta ggtttaatta
     4561 agcctaaaag cattcttata gtagttgttt tgcctgcacc atttcttcct aaaaaaccat
     4621 aaatttgtcc actttcaaca gttatactca aattattaac tgctatctga cttccatatt
     4681 gctttgttaa gttatttatt tcaattatat tattcaaatt tttcacctct ggcacctttc
     4741 ttagtttgtt gttaccttta tcttacgcgc gattagaaaa ggctgccttt atctattctt
     4801 acaaaaacag ccgctaatct tacaaaaatg aaagattgga gttatttaaa ttgaatggtg
     4861 aatattgaac catcgtttaa tgtagatgta gcacttactt tatggcctaa gcgctttgag
     4921 attgtgctga caatatataa accaataccg cttgatttct cacttattct accgttatag
     4981 cccgagaaac ctttatcaaa gattttcggt aaatcactcg cattaatgcc gataccgtta
     5041 tctcggattg ataatttctt tgtattttcg tcaaacgtaa tccacacatc tttgccacgt
     5101 gcatatttta atgcattgtt taatatttgc tcaatcataa ttgatgccca tttggcatct
     5161 gttaatataa ccgcttcact tttctcataa tgcaactttg tttgatgatg aataaactgt
     5221 atcgcataac gtttaatcag tggtcgaatt aattcatcta cactcacttc cataaagacc
     5281 atgtatgtct tttggttcat gagtttcaaa tagttcagtg caagattcgt gtaattatca
     5341 atttgtaaga cttcattacg cacgttagaa atcacgtgtt catcaggatc ttcaagcagt
     5401 aactttgatg ctgtaatcgg tgtcttgatt tgatgcaccc acattaaaaa atatgactct
     5461 aactcacttt ggtagttaat aaagtcatta cgaagtttat ggttttcgat tcttaaatct
     5521 tcgttctgtt cctgtagtgt ttattctttt ttaaagttca tgtaatggat accccagtaa
     5581 atcattgaaa caaacaatac aatacctaga ccaatcatat atgcttgcat cggcacatta
     5641 tacatgaata atataaagta gaataaagca caagtaagga tgacaatata aatttcacgt
     5701 tggattgatt tcaagaaatt catacgtcac ctacacgata tccgacattt ttcttcgttt
     5761 cgataaaatc tgtaagtcct agtttttgta gctttttgcg taaacgtgta atgttgacag
     5821 caagcgtatt gtcatcaatg aagtgttctg attcccagca tttttcgatt aacgcattac
     5881 gactgacgaa tttgcccttg ttctggaata agctatgcat gatttgcaat tcagtgtgtg
     5941 tcaggtctat ggtttcttca ttaaatttaa gctttgctgc atcaagtagt aactcgcaat
     6001 catctactgt taaattatta acttgagtgg tgaaatcata cgtgcggcgt aatagtgctt
     6061 gaatcttagt aattgtaaca gtcatattaa atggctggtt ctgttgcaaa gtaaaaaata
     6121 tagctaatca ctaatatatc atgtcagtgt tcgtttaact tgctagcatg atgctaattt
     6181 cgtggcatgg ggaaaatccg tagatctgaa gagacctacg gttcttttta tatagagcgt
     6241 aaatacattc aatacctttt aaagtattct ttgccgtatt gatactttga tatcttgtct
     6301 ttcttacttt aatatgacgg tgatcttgct caatgaggtt attcagatat ttcgatgtac
     6361 aatgacagtc aggtttaagt ttaaaagctt taattatttt agccattgct gccttcgttg
     6421 aaggtgcctg atctgtaact accttttgag gcttaccaaa ttgtttaatg agacgtttga
     6481 taaacgcata tgctgaatga ttatctcgtt gcttacgcaa ccaaatatct aatgtatgtc
     6541 cctctgtatc aatggcacga tataaatagc tccattttcc ttttattttg atatacgtct
     6601 catcaatacg ccatttgtaa taagcttttt tatgcttttt cttccaaatt tgatataaaa
     6661 ttggggcata ttcttgaacc caacggtaga tcgttgaatg atgaacgttt acaccacgtt
     6721 ctcttaatat ttcagatata tcacgatagc tcaatgcata tcttagatag tagccaacgg
     6781 ctacagtgat aacatccttg ttaaattgtt tatatctgaa atagttcata cagaagactc
     6841 ctttttgtta aaattatatt ataaattcaa ctttgcaaca gaaccgtatt ttatgagtac
     6901 actctaaatt aaggagatgt ataatcaatc taaaattgta tttttatact tttgttatat
     6961 aatctttcta cttagagata tattgtaaaa aaaccaacaa aatgttatat taacattact
     7021 attttatcga tgaaggagga ttaaaatgac agcttctatg agactcaaat aaaccttttt
     7081 gaatacaaaa ctaaattaat tcaaaaaggc ttgtccgcaa tacacactca taggatgact
     7141 tatgtcattt tttatccttc gataggaata ataaacgctt tcaaatacta aaacatcata
     7201 ataaattttt aagtgtttga aagtgtaatg tgaatgagac gggagtagcc tgtctcattt
     7261 ttttgcgtac acgtccttta aatatacaag gagtgtataa atatggaaca atatacaatt
     7321 aaatttaacc aaatcaatca taaattgaca gatttacgat cacttaacat cggtcatctt
     7381 tacgcttacc aatttgaaaa aatagcactt attgggggta atggtactgg caaaaccaca
     7441 ttactaaata tgattgctca aaaaacaaaa ccagaatctg gaacagttga aacggttggc
     7501 gaaattcaat attttgaaca gcttaacatg gatgtggaaa atgattttaa cacgttagac
     7561 ggtagtttaa tgagtgaact ccatatacct atgcatacaa ccgacagtat gagtggtggt
     7621 gaaaaagcaa aatataaatt agctaatgtc atatcaaatt atagtccgat attactttta
     7681 gatgaaccta caaatcactt ggataaaatt ggtaaagatt atctgaaaaa tattttaaaa
     7741 tattactatg gtactttaat tatagtaagt catgatagag cacttataga ccaaattgct
     7801 gacacaattt gggatataca agaagatggc acaataagag tgtttaaagg taattacaca
     7861 cagtatcaaa atcaatatga acaagaacag ttagaacaac aacgtcaata tgaacagtat
     7921 ataagtgaaa aacaaagatt gtcccaagcc agtaaagcta aacgaaatca agcgcaacaa
     7981 atggcacaag catcatcaaa acaaaaaaac aaaagtatag caccagatcg tttaagtgca
     8041 tcaaaacaaa aaggcacggt tgagaaggct gctcaaaaac aagctaagca tattgaaaaa
     8101 agaatggaac atttggaaga agttgaaaaa ccacaaagtt atcatgaatt caatttccca
     8161 caaaataaaa tttatgatat ccataataat tatccaatca ttgcacaaaa tctaacattg
     8221 gttaaaggaa gtcaaaaact gctaacacaa gtacgattcc aaataccata tggcaaaaat
     8281 atagcgctcg taggtgcaaa tggtgtaggt aagacaactt tacttgaagc tatttaccac
     8341 caaatagagg gaattgattg ttctcctaaa gtgcaaatgg catactatcg tcaacttgct
     8401 tatgaagaca tgcgtgacgt ttcattattg caatatttaa tggatgaaac ggattcatca
     8461 gaatcattca gtagagctat tttaaataac ttgggtttaa atgaagcact tgatcgttct
     8521 tgtaatgttt tgagtggtgg ggaaagaacg aaattatcgt tagcagtatt attttcaacg
     8581 aaagcgaata tgttaatttt ggatgaacca actaattttt tagatattaa aacattagaa
     8641 gcattagaaa tgtttatgaa taaatatcct ggaatcattt tgtttacatc acatgataca
     8701 aggtttgtta aacatgtatc agataaaaaa tgggaattaa caggacaatc tcttcatgat
     8761 ataacttaaa aactatacac taatttatat tgaatattag gttcatttat atcaatataa
     8821 aaaatacgtt cacaagaaaa atataataga aataaggatc caataaaatg actcgacata
     8881 atgaaattat taaatgtgca gaaaaatatc aattacacat ccaacctcaa acaatctcat
     8941 tgaatgaatc gggacttgat tttcaagttg catttggaaa agataaacat ggagtagaat
     9001 gggttttgag actaccaaga agacctgacg tttataaacg aacaaaaccc gaaaaacaaa
     9061 cggtagactt cttacagaag aatgtttcat ttgaaatacc gaagtggaaa gtacatgcaa
     9121 aagaccttat tgcttaccca aaacttacag gtaaacccgc agccacaata gatccagaaa
     9181 tacaaaatta tgtatgggaa attgaacaca aaccattacc agaaaacttt attaacacat
     9241 tagctgaaac actcgtagat ttacacaaca taccagaaga aaacattaac gttcagcata
     9301 taaatatcaa aaccatacaa gaaataaaaa atgactttca aagaagaatg aataaagtta
     9361 aagaaactta tggcgtatca gatgaattat ggaacagatg gaaacaatgg ttagaaaacg
     9421 acgaactatg gcctcgacat gcgaccatga tacatgggga cttacatcca ggacatataa
     9481 tggtagataa ccaagcaaac gtcacaggtc tcatagactg gactgaagca acccactccg
     9541 acccatcaat ggactttata ggacaccatc gtgtattcga cgacgaagga ttagagcaac
     9601 ttataacagc atacggtaaa gctggaggtg aaatatggcc acgaatgaaa gagcatataa
     9661 tagaactcaa tgcagtattc ccaatgttta tcgctgagtt tgctatggaa tcaggagaat
     9721 cggcgtatga aacgatggca ttgaaagagt taggtatgaa agagtagaac ttatattaaa
     9781 agaggcccat tttaaagacg aaggcaatcc tgatgaaatt ttaaaactgc gtttaattag
     9841 tgatggtctt tggttttcta taatgtatta aatatctgga catggaacaa atgcaacaaa
     9901 tggaaagaat cgttttcgaa atttctaact ccttaaatca ggaggataaa taaacggtca
     9961 ttttcattga aagcacttat ataaatttca tcaattttaa tacctaccgt aaatacattt
    10021 cataagtatt aaacagatga ttaaaatttt tagttaattt aaatatttat gatagagatg
    10081 accttcaaat gaacggttct gttacaaaga aaaaatctat atttattttt agtaagtttt
    10141 aattatagat atgtacactt tgagtacacc ctaaccatac aaaaagaatt gttgaaatta
    10201 taacaattaa aacgtatgat tagggtgtat atttattatg gagtgaagat gaatgattat
    10261 aggttatgcg agagtatcat cgatagatca aaatttagaa agacaattgg ataatttaaa
    10321 gacgtttggt gtggagaaaa tctttacaga gaaacagtcg ggaaagtcag tggaaaatag
    10381 acctgtattt caagaagcac tcaattttgt cagaatggga gatcgattcg ttgtggaatc
    10441 tattgatcgt ttaggacgta actatgatga aattattgaa actgtgaatt atttaaaaga
    10501 aaaagacgtt cagttaatga ttaccagctt acctatgatg aatgaagtaa ttggcaatcc
    10561 attactggat aagtttatga aagatttaat cattcaaata ttagcaatgg tttcagaaca
    10621 agagagaaat gaaagtaaac gtcgacaagc gcaagggatt caagttgcga aggaaaaagg
    10681 cgtatataaa gggcgccctt tactttattc accaaatgct aaagacccac agaaacgtgt
    10741 tatttatcat cgcgtggtgg aaatgttaga agaaggcaaa gcgattagta agattgcgaa
    10801 agaagttaat atcacaagac aaacagttta tagaattaaa catgataagg gattatcttg
    10861 ataaattgat tgtcaatgca aatgagcgtc tctcacagtg tctcaaattc cataacatat
    10921 tattgaattg agattttaat aattatacaa gaaaaaagag tacgagttcg cagctttcta
    10981 tatagtaatt gttttactaa taaggtgttt taaaatatat tataatgtaa aaagtatttg
    11041 acatttgtat caaaaatatt tacactcata aatgtcaaat actttttaca tttatgaggt
    11101 gttataatga atttaattcc ttttattatt attttaattt tttcttgttt atggacggct
    11161 ataactaaca aatatttacc ttcagaaaca ttagataaaa aaggacaaca agatcgatac
    11221 gatgaaagac aacgtaaaat gtttattgaa atactggcta aaagttttat ttggattgta
    11281 tattgtatgt tatttacact agtgcttaaa tttacaggtt tgtcaccaag tattgaagga
    11341 tcttggtttt ctcaatatcc agaaattttc tttataattg tagcactatt tcttatgctg
    11401 tttaattact atactacaaa taaaaaatat acatctaaag gttaaattaa tgaaaaatag
    11461 aattagagaa tatagaaaac aaaaaggaat ttctcaagaa actttaagta aagaattaaa
    11521 agtgtctagg caaactatta atgcaataga aaacaataaa tatgatccta cattgacatt
    11581 agcatttaag ttggcaagat tgtttgatac aactgtggat gaattatttt cttaaccatt
    11641 catttatcac aaaaagagag tgcgaattcg cactctcttt ttgtgtagta gtactattaa
    11701 attttgtctc aataacgaac gtttataacg caatcatgat gagagtatta atacagttaa
    11761 ttatttgaaa tactttagtt tacataatcg atgattacca gaagttctca ctatataaaa
    11821 gacactaaaa ttttataact tttagtgcct aatcaattat tttagctacc aaacaaacga
    11881 ctccaaaacc cctttttagg ttccgtttct tcactctttt cttctatatc actatcattg
    11941 tcatttagat tagctggaac gtcttctttt tttgcttctt caccttgtgt actttcgtcg
    12001 ctttgacttc cagatatatc cttatattga atctctgttt ctttagtttc ttcctgatct
    12061 ctgttaatat ttacaggatt agtagtatat gttttttctt gtgcatcaac gttttgtcta
    12121 gtgttagtag atgtatcaaa gggataatta aggtgttttt ctttttctaa ttgattttct
    12181 aatttttgaa tctttttatt actctctaat gccagaactt gctgattttc aagcaatttt
    12241 gatctttctt gcaactcttt tctgtaaaat tcaattgttg tttcatgacg actttcttgt
    12301 ttttcaattt gattatttaa agtatcaatc tgatttttaa ggatttctac tatttgagca
    12361 tcgcttttcg attcaaaatt aatattatcc gacccttttt tttgtttgtc ttcagtagta
    12421 ctttcaaaac catatttctt tttattttca tttactcttt gaatgatttt ttctatatcg
    12481 gtatcttctt taataaacga agtgttttcc tgttttattg tctctatatt caatcgctta
    12541 atattattaa aaatcgtttg cttactaacc cctaattctt cactcaactt tttcacactt
    12601 ttcatatcat caacccttta taaaatgttt ataaaccctt gataaaatat tataagctta
    12661 taatgcttaa taataaagtg attaataaat aaaaataaac atccatcaac ctttttatca
    12721 agggtttata aacccttgat aaagctatat tttccctttt ataagtttat ttaaagtgct
    12781 ataataaact taatataaca aaaaccccaa ctactggtaa tagtcggggt ttggtaataa
    12841 aacgtttaga aactgtttat ttatataatt ttcgatactt gtattatata atgaataata
    12901 tcttagtgca agcacctaaa tcattaaata aatgtaaagc taaataaaaa cgttttatta
    12961 cctcttacca aactaaaata tggaaagagg ttttttgtca tgcaaaatca atattttaca
    13021 gtgcaagaaa attacaaaga aagattttat caaatcccta aagtattctt taccagtgaa
    13081 aattataaaa atctaactaa tgatatgaaa atagcctatg ctatcttacg tgatcgctta
    13141 aatttatcta ttaaaaatag ctgggttgat gaagatggta acatttactt tgtttattca
    13201 aatgaaaagc taatggaaat cttaaattgt aaaaaagaaa agttaacaaa aataaaaaaa
    13261 gggttagaaa acgatggtct actcattcaa aaacgacgtg gtttaaataa accaaatatt
    13321 ctatatttaa tgaaacctat tgtcacagaa cgtgatattt acaaaataga aaaagaagaa
    13381 aacgatgttg aaccttacgg cgagaaggaa gttcgaaaat cgaacgtcca gaagttcgaa
    13441 aatcgaacgt ccagaagttc gaaaatcgaa cgtccagaag ttcgaaaatc gaacactaat
    13501 gatactgact ttaatgacat tgactttaat gatactgaga ataatgatat gaatgacttg
    13561 aatgatataa agtacaaaaa tgaaattagt aatcattcaa atcattcaaa tcaatttaca
    13621 cataattttg atgataaaga aatgctattg caagaattcc cagaacaact aactaattat
    13681 ttattaaaat atgattatag agatttagaa atcataaagg ctgttattct taaagcaaaa
    13741 aaatcattta attcaaggca tgaagatatg cattacatgt tagaggacat tgaggatgaa
    13801 atattaactt ctttaaaaag acttaaaaaa gctatacatg ataggggagt taaaggccaa
    13861 aaggaaacaa tcaagtcaat gcaagcttat ttaatgcaaa caattctaac tgaacttgaa
    13921 gaaacacatg ccctttatat gcgccgtaaa aacatgaaac aatataatat ctttaatcaa
    13981 taattcaaat gattttataa cactcgaaga gacatcaaaa tattccgtta aatcatctga
    14041 ttttttaact gatgatgatg aaaaaaatca agaaattgta agtgatttag aaaaaggttt
    14101 gtatcgaaaa cgtatgttaa gttatggtgg attgcttaaa caaaaacata aaattttaaa
    14161 cttagacgat gtcgaagatg gtaatttgat taatgcaagt gatgaagata aaacaacaga
    14221 cgaagaagaa aaagcacatt caattaccgc aatttggaat ttcgaaaagc aaaattatta
    14281 tttaagacat tagtgttgac taatgtcttt tttgttgatt ttttataaaa aagtactgtc
    14341 ttatttttgt gacaaatgct gtatgtagtg tcacaaaagt gtgacagtac agctttgtat
    14401 gatatcactt taaaatacct taaaacccct ggaatttctg gctttgccag acctatcatt
    14461 tttgaatgat agcaaattct ccttatgctc ttacggagtt tttagagaaa aattaaaaat
    14521 tctcgatttt tgataaaaaa cgccctgcag gaatttagaa aaacatgtgg aagttttaaa
    14581 ggattttatg ctaattttta atttgcatgt aaatgggcag tgttaaaaaa ttagtcactg
    14641 gttttgctga aatttttgtt tgtgaaatta ccatatattt atttggctca tatttgcgtt
    14701 ttaagagctt gtatatattt ttaagtacaa aacctatatg atttgcctct aagtcttcaa
    14761 aatgcctctt aaaatccaaa ataaaagtgt ttaatattgt aataaggatt gctattttta
    14821 taaaaggaga gatatcatga agattaaaac tttcgctact tttactcatc gtcaattaga
    14881 taaacaagtt aataggtttt taaagaatga tattcatgta ttagaaattc aaacacacag
    14941 taattggtta tatatttcag ctattgtttt ttatgaggat aaataaggaa catcgtattt
    15001 atgccgaaaa aatttattga tgttgagaag aacccttaac taaacttgca gacgaatgtc
    15061 ggcatagcgt gagctattaa gccgaccatt cgacaagttt tgggattgtt aagggttccg
    15121 aggctcaacg tcaataaaac aattggaata aaatcaacct acagagcatc cagctgcaaa
    15181 tgcagttccg caaactgctt cacatacacc gccggctatt ggatttacga ttgcccatac
    15241 tccacagtag tgagcacatg cataaagtcc agccatacta caagcgaatg ctttgccgtt
    15301 ccatttgaat cctgcatatg ttaccggtct gtttttatca actttttcag ccttatattc
    15361 aacgtaattt ttgttaataa gtatttttcc ctctttgttt tcagtcatac ttcttttttc
    15421 ggcaatgaat tttgtgattt ttttgttgta gttatcatat attgtttcag aaactacaat
    15481 ttcttttgtt ttttcatttt tataagcgtt attagctatg tagccatatg catttttatc
    15541 atttgcttct gtagctagat attttaattt agaatttttg tcttctagct ttattttttt
    15601 aaatccttct tttttcattt gcttttcgaa tttttgcata tcttcatttt tattttgcaa
    15661 ttgttctaga aaacttattt ctttcttggt catttgtgat acttcgatgt ttttgctttt
    15721 caatttttct ttttgatttt ctagtaaaga acttttttgt tcttcatttg cgtaagcatt
    15781 agaaaaattc gcgctaaata ccagtaggaa caaggaaatt attgaaaata attttataat
    15841 tttcatttga tacctccaag ataataagat attttaattg tataagtaag cgctttataa
    15901 aatcaatatt tattttaata tatttataaa catatttttg aaaggagtct attttatgaa
    15961 tgaaaattta gaaaaatata taaaaagttt gccactttta ggtttgttaa ttagtatttt
    16021 gttaattgtt ttattctttt ttattttaga tgttgatgaa gatttattcg tggttttttt
    16081 atactgttta ttgcctttga tagttaattt gtctatttat ggtttttatg tttttgttaa
    16141 aagaaagtaa gattatgaat aattttgttt ttaatttgta tgctttgagg gggttggaaa
    16201 acggtaggtg tatagattag atttcttgtt tttttgaaac gagtgtgaac gagtttcttc
    16261 ttgtcttgat ttttgataaa aacgccctac aggaatttag aaaaacgtgg ggaagtttta
    16321 aagaatttta tgctaatttt taatttgcat gtaaatgggc agtgtctaaa aatagttatt
    16381 gtttttgctc aaatttttgt ttttgaaaat agcatatatt tatttggctc atatttgcgt
    16441 tttaaaagca tatataagtt ttagatataa aactatatga atcaccccta aatctttaaa
    16501 atgccctctt aaaattcaaa ataagggcat aaaaaataaa agctacccca gataaagtga
    16561 ggtagctttt tctttcatac ggtctattta tttttctttc gacgccgtat cgtcgatgta
    16621 attcaaaaaa gttatgggct ataaatctac atcacttttt caagaatgta gtagcaatat
    16681 tcaacgcaaa ttaattttta taactgctcg gaatatctca agccgtttct ttaattttga
    16741 aataaaaaaa tcgacgaagg tcgattacgt ttttgcacat gtcgatttat ccgacgtata
    16801 agtgcgccct tacgggattt aactagatta taacgacgaa ttttagacct gtaaagcaat
    16861 aacaattagg tctaccaggt agataaagtg tacaaagtag aaccgaaaaa gttatttatt
    16921 ttgaagattt ttcgtagata taatttgcta ataaacttcc taaaactgaa taaagaaaaa
    16981 tagttaaact catgtaatca ctccataatt ctgaagtgtc tccatgtcta cgcatagaca
    17041 cgcatttatg ccgaaaaaat ttattgatgt tgagaagaac ccttaactaa acttgcagac
    17101 gaatgtcggc atagcgtgag ctattaagcc gaccattcga caagttttgg gattgttaag
    17161 ggttccgagg ctcaacgtca ataaagcaat tggaataaag cacctatgat tatttaaaaa
    17221 tataagttat aatacattca aaaatatttt tgaatgaggt gtattatgat tcaaacggtt
    17281 gttgctgctg ctgttcttta tattgctaca gcagttgatt tattagtaat tttattaata
    17341 ttttttgcta gagcaaagac tagaaaagaa tatagagata tttatgttgg tcaatattta
    17401 gggtctatta ttttaatatt agttagtttg tttttagctt ttgtattaaa ttatgttcca
    17461 gaaaagtgga tattaggttt attgggttta ataccaattt atcttggaat taaagtggct
    17521 atttatgatg attgtgaagg agaaaagaga gctaaaaaag aattgaatga aaaaggattg
    17581 tctaaattag ttggtacggt tgcaattgtt acgatagcaa gttgtggcgc cgataatatt
    17641 ggtttatttg ttccgtattt tgtgacatta agtgttacta atttattact tactctgttt
    17701 gtctttttaa ttttaatttt cttcttggtg tttactgcac aaaaattagc taatattcca
    17761 ggaattggag aaattgttga gaaatttagt cgttggatta tggctattat ttatatagct
    17821 ttaggtttat ttattattat tgaaaatgac actattcaaa caattttagg atttatattt
    17881 taatttaggg tgtgatttta tatgagttat gaaaatactt gtgatgtgat ctgtgtacat
    17941 gaggataaag ttaacaatgc tttaagtttt ttagaagatg ataaatctaa gaaattactt
    18001 aacattttag aaaaaatttg tgatgagaag aaattgaaaa tcatattatc tttgattaaa
    18061 gaagatgagt tgtgtgtttg tgatatttct ttgatattga aaatgagtgt tgcttcaact
    18121 tcacatcatt taaggctttt atataaaaat gaggtacttg atttttataa agatggaaag
    18181 atggcatatt attttattaa agacgatgaa ataagagagt ttttctctaa aaatcatgag
    18241 ggtttttgaa acgagtttct tcttgtcttg atactataaa aaacaaagtt atataacata
    18301 agacgactta tcggaagtag atatgcttgt atattcacag atggtataat aatagagtcg
    18361 cctatctctc aggcgtcaat tgacgaagag aggaggtgca ttactttgct aatcattttc
    18421 gttcacatca tagcaccagt catcagtggc tgtgctgttg cgtattatac ttattggctt
    18481 agtaaacgca acaaataatt aataggcgac tttataagcc agcatcgaaa aaatccccac
    18541 actactgcaa tagtgtgggg attggtgcat taattgttaa tcattttcgt tatataaatt
    18601 ataacactgg ttagtagaga aatgcaaaat gattgaatta atagtaaatg tattaatgaa
    18661 tactcacact tatactttta aaatagttct tcctaaatgt tgtcctttta aaagctgatc
    18721 tatcgtttta tagaattctt caaatccaat ttcattgtat agaagtgaat ttcctatatt
    18781 ccattcattt gctaacttac tccatatatc ttcacggata ttatgatcaa catatacaga
    18841 atcaatgcca attaatttaa tacctctaag aataaacggt aatatattag cgttaagtga
    18901 atttcctgct acatttccac atacagtagc aacaccttga taatttagac gttttaaacc
    18961 gtataatgtt acattactcc ccactgtatc taagaaataa tcaaaaatac cttttttaag
    19021 cggtttatta tcatcttcaa ttattattac ttgttttgca cctaattttt ttgcgatttc
    19081 tatttgcttt tcttttctta cgatagcagt gatattttca tatccaattt tatgtaaaat
    19141 ttgtatagca acactgccca caccacctgt agccccagac actaaaattt caggttgtga
    19201 tttgatattc attccatatt tttctagttc aaaaattgat aatgctgccg taaaaccggc
    19261 tgtgccatat accatagact ctttaataga catttcttgg ggaataggaa caatccactc
    19321 tccaggcact tgtgcatatt ctgaaaatcc acctgtatgt gtcacgccaa catcataacc
    19381 agtaactaat acattatcgc cttctttaaa cttatcattc gaacttgatt caacaatacc
    19441 tgcaaaatct ataccaggaa tcataggata atctctaatt acaccgcctt tatattgtaa
    19501 agctagcata tccttataat ttattgaaga gtataccact tttactaata catcaccctt
    19561 cgataaatga gattcatcaa ctttttctac tttataacta acatttgatg cactttttct
    19621 cactactacc gcattgaaca tttattagcc ttctttcaca attgttttgt agaagtatgt
    19681 ttttgttgca aaactatttt cctacaaaac ttatcatgat atgataagcg agtcaatact
    19741 aatgatttaa gagaggtttt agttgtggaa ttagataatt gtataaatta tttgttgagt
    19801 acttctcaga atagagtttt taaacatttt aaaagtttat taagtcccta taatattact
    19861 ccagcagaat atggtgtttt gaattgcttg attaatagta atttgaatac acctaaagcg
    19921 attggtaata ctttaaattt agaaccttca acaatatctg gaatattaga taagatgtcc
    19981 aaaaaggatc taataacacg tcaaactgat aatgaaaata gaagaactgt gctaataaac
    20041 agtacaaata aagcaaaaga cttatggcca acattagaaa agaaagccta tcaacttaat
    20101 aatcattact tcgctaattt aactaatgaa gaaactgtag tgtttaaaaa gatattacta
    20161 aagataaata aaactacgtt ttaattttta cgttataaaa aagttgaagt gcgaattata
    20221 atcgagtaaa tataaatgat agcttagctt aattatatat taaagccaac tatactttta
    20281 ttcccgaaaa acctactatc caaatttcta agatacttta gcctctataa taaacatatt
    20341 gtaatataat ttatgtatta tttaggttta ccctaatcga acaataaaaa atacttgtaa
    20401 aatgctattg tcactttgct tgatatatga gaattattta accttataaa tgagaaaaaa
    20461 gcaacgcact ttaaataaga tacgttgctt tttcgattga tgaacaccta taattaaact
    20521 attcatctat tatttatgat tttttgtata tacaatattt ctagtttgtt aaagagaatt
    20581 aagaaaataa atctcgaaaa taataaaggg aaaatcagtt tttgatatca aaattataca
    20641 tgtcaacgat aatacaaaat ataatacaaa ctataagatg ttatcagtat ttattatgca
    20701 tttagaatat taaaattcct tcattacact cttggcggtt tcacttatca acttatcatt
    20761 tggcttatca cttttattgt ctttattcgt aaaaatgact aaaacaatag gttcagattg
    20821 gcccttagga taaacaaaag caacatcatt tctagaagca tatgttattg cttgaccact
    20881 tttatcagca accttatagt cttttggaac accatcttta attaaagtgt ctccgttttt
    20941 attattaaac attaaatcaa gtaagaaatt tttatttttt ttgcttaatt ttccatttgc
    21001 gataagttta tttaaagtct tgccgaaagc agcaggcgtt gaagtatctt ttttgctctt
    21061 tggtgaatag taatttaatt ctatctcata tctaactgga tttgttactt tatctcctag
    21121 ttcttttaga cgttgtttaa cttttttgat tccaccgatt tcttttataa ttttattgtt
    21181 tgctgtatta tcactatatg tcattgaagc ctcaataagt tcttttaaag tgatatcttt
    21241 tcctacatat ttttctaaaa taggagaata agcaactata tcatctttgt taatatgtac
    21301 ttttttattt aacttattat aaggtacttg ttctaacaaa atagcactat ttatcgcttt
    21361 tgaagtcgaa gcataggcaa atctcttatc tgaattaaat tttacttcct taccactttt
    21421 agtatttaaa gcataaacac caatatgagc attatatttt ttttctaaat catttaactc
    21481 tttggcatgt gaactgtttg aattacatgc acttaaaact aaagcaatta caattaaaaa
    21541 tattaacttt ttcaaaataa accctccgat attacagttg taatttttat aaatcaataa
    21601 tattacaatt gtaatattga tgtcaattgt ttattataaa tatttgaaag aaggtgtcaa
    21661 aatggctaaa ttactaataa tgagcgtagt aagcttttgt tttatatttc tattgttagt
    21721 attttttagg tacatattaa aacgctattt taattatagt ttaaattata aagtttggta
    21781 tctaactatg cttgctggat taattccttt tatcccaatt aaattctctt ttataaaatt
    21841 taataatgtg aataatcaag cgcccacagt tgaaagtaag tcacacgatt tgaaccataa
    21901 cataaatacc accaaaccta ttcaagagtt cacaacagat atccataagt ttaattggga
    21961 ttcaattgat aatatctgca cagttatttg gatagtttta gttattattt taagttttaa
    22021 atttttgaaa gccttattat atcttaaata tttaaagaaa cagtcacttt atctaaacga
    22081 aaatgaaaaa aataaaatag atacgatact tttcaaccat caatataaaa aaaatattgt
    22141 gattcgaaaa gcagaggcta ttcaatctcc aataactttt tggtatggga aatatattat
    22201 tttgattcct agttcatatt ttaaaagtgt aattgataaa agactaaaat atataatact
    22261 gcatgaatat gctcatgcta aaaatagaga tactttacat ttaattatct ttaatatctt
    22321 ctctattatt atgagttaca atccattagt acatattgta aaaagaaaga taattcatga
    22381 caatgaagta gaagccgata gatttgtcct taataatatt aacaaaaatg aatttaaaac
    22441 ttatgcggaa tctattatgg actccgtatt aaaaacccca ttttccaaca aaaatatatt
    22501 aagccattca tttaatggta aaaaatcatt actcaaaagt agattaatta atataaaaga
    22561 agccgatctg aaaaagcagt caaaattaat tctaattttt atttgtattt ttactttttt
    22621 tatcatgata attcaaagcc agtttttaat ggggcaatcc ttaactgatt ataattataa
    22681 aaagccctta caaagcgatt accaaatttt agatgaaagt aaaaattttg gttcaaatag
    22741 tggatctttc gttatgtaca gcatgaagaa agacaaatac tatatttata atgaaaaaga
    22801 aagtagaaaa agatattctc ctgattcaac ttacaaaatt tatttagcac tgtttggact
    22861 tgaccgtcat attataagtg ataaaaattc acgtatgtca tggaatcata aacattatct
    22921 tttcgagtct tggaataagg aacaagattt aaatacagca atgcaaaatt cagttaattg
    22981 gtacttcgaa cgtattagta atcaaatacc aaagaactat actgcggctc aactcaagca
    23041 attaaattat ggtaatgaaa atttgggaag ttataaaagc tattggatgg aagatagttt
    23101 gaaaatatct aatcttgaac aagtaatagt ttttaaaaat atgatggaac aaaataacca
    23161 ttttagtaaa aaagcaaaga atcaattatc ttcttcatta ttgattaaga aaaatgaaaa
    23221 gtatgaactg tatgggaaaa caggtacagg tatagtaaac gggaagtata ataatgggtg
    23281 gtttgtaggt tacgtaatta caaatcatga taagtattat tttgctacac atttatcaga
    23341 tggaaacccg tctgggaaaa atgctgaatt aataagtgaa aaaatattaa aaggaatggg
    23401 tgttttaaat gaccaataag caagttgaaa tatctatggc tgaatgggat gttatgaata
    23461 taatatggga taaaaaatca gtatcagcta atgaaattgt agttgaaatt caaaaatata
    23521 aagaagttag tgataaaacg attagaacat taatcacaag actatataaa aaagagatta
    23581 taaaacgata caaatcagaa aatatttatt tttactcatc aaatattaaa gaagacgata
    23641 ttaaaatgaa aactgctaaa acctttctta ataaactgta tggaggggat atgaaaagtt
    23701 tagtgctgaa ttttgcgaaa aatgaagaat taaataacaa agaaattgaa gaattgcgag
    23761 acattttaaa tgatattagt aaaaagtaat tagaggtatg tttcaaacat aatacatatg
    23821 taaaccatac ttgactctta ttaaattagt tttgttaatg acatttaaaa tatattatta
    23881 aagatgagag tgtccagaaa ataaccattt tctgtacact cttgtttttt gtctaaataa
    23941 gtaagtatta catagaatta tgtgttcaaa aacccaacaa aaaatctatg ttcacaaaag
    24001 acctcgtaat caagttatgg tacactaaag gtgtatatag gaggtctgta ttttgaaaat
    24061 aggttatgca agagtttcaa ctggattaca aaatttgaat ttacaggagg atcgattaaa
    24121 tacatatggt tgtgaaaaga tatttaatga ccatatgagt ggttcaaaaa gtaagcgacc
    24181 tggtttagat aaagcaattg aatttgcgag gtcaggagat acgattgttg tttggagatt
    24241 agatagacta ggacggaaca tggaggattt aatcacgtta gttaatgaac ttaacgaacg
    24301 aggtgtgagt tttcatagtt tagaagaaaa tataacgatg gataaatcaa gttctacagg
    24361 acaattatta tttcatttat tcgcagcatt tgcagagttt gaacgtaatt taattttaga
    24421 gcgatcatca gccggtcgaa ttgcagcacg tgctagaggc cgatatggag gtagacctga
    24481 aaaattaaat aaacaagatt taaacttact taagacactt tatgataatg gtacgccaat
    24541 taaaacaatt gcagagcagt ggcaagtttc acgtacaact atttatcgtt atctcaataa
    24601 attagaggca aaagagaata aaaacaagta gagcaactaa tctagcttac tataatgaat
    24661 ctactaaata attttatata tcattaacat acaaagactt tgattcaatg tcactcgatg
    24721 taaaagagac gttctgacag cgtctaaaat ctcataatat tttcctgtta tgagatttta
    24781 ataataacac aagaaaaaag cgtacaaatt cgtacgcttt tttaatataa tacctgtttc
    24841 actaatgaac gttttaagta tcttcatcat taactgaaac tacagtaata atactcgtca
    24901 acaatattag tatagttaaa atagaaagac ttaggactgg cataaccgaa ctaaaattat
    24961 atttgaaata taaaagtgct aatgaagaga gaaatgtaaa aataataaat accaaagacc
    25021 atttttctaa tttattcatt ggcttcacct ctttgaactt ttatataatc ataggagata
    25081 tttttactac gatttataaa aaataagaag ataatcatta ttaaagaaac gattaatata
    25141 atgttgaatc ctatagtatc acctaattcg tttacacctt tgcctaatat cggagataca
    25201 aagctttgta ttaataatga aagagatgcc catatagtaa atgaggttct gttgcaaagt
    25261 tgaatttata gtataatttt aacaaaaagg agtcttctgt atgaactatt tcagatataa
    25321 acaatttaac aaggatgtta tcactgtagc tgttggctac tatctaagat atgcattgag
    25381 ttatcgtgat atatctgaaa tattaaggga acgtggtgta aacgttcatc attcaacggt
    25441 ctaccgttgg gttcaagaat atgccccaat tttatatcaa atttggaaga aaaagcataa
    25501 aaaagcttat tacaaatggc gtattgatga gacgtacatc aaaataaaag gaaaatggag
    25561 ctatttatat cgtgccattg atgcagaggg acatacatta gatatttggt tgcgtaagca
    25621 acgagataat cattcagcat atgcgtttat caaacgtctc attaaacaat ttggtaaacc
    25681 tcaaaaggta attacagatc aggcaccttc aacgaaggta gcaatggtta aagtaattaa
    25741 agcttttaaa cttaaacctg actgccattg tacatcgaaa tatctgaata acctcattga
    25801 gcaagatcac cgtcatatta aagtaagaaa gacaaggtat caaagtatca atacagcaaa
    25861 gaatacttta aaaggtattg agtgtattta cgctctatat aaaaagaacc gcaggtctct
    25921 tcagatctac ggattttcgc catgccacga aattagcatc atgctagcaa gttaagcgaa
    25981 cactgacatg ataaattagt ggttagctat atttttttac tttgcaacag aaccagcatt
    26041 tgttttaata tttgttccaa agagccatca tggctttgac agagctcttc ttctatgcaa
    26101 atgcttttaa tccgatccac acgaaaattc ctatattcat ttcttagcct gcaaaaccga
    26161 ttaagtacca gttctgttca taaaacccca gtgatatagg ctctatcatt ctgctttccg
    26221 gttcttgccc tcctgatgca ggatattgaa ttgatattac ccttttattg cagattgcag
    26281 tctgaattgc tgcaataacg ttatccgcca atgtattttt ctgtccagat gtgctgtaca
    26341 cgttaatact gttctccaac tcctgtgcat agtttttttc accatgcttc aaaatagatt
    26401 tgattttata catagctgaa tcaaaatcct ttataaatga ttcatctcca tggcaattta
    26461 aaaatttccc tgctgtaatc aatgcaccca cttccgccgc tgtaaacatt accggcagaa
    26521 gtaggaagcc ctctacaaga aaatatcctt ttccggcctc agatccaatt ggtattccgg
    26581 cttcttcaag tgtccggata tctctgtaaa ctgtccttaa gcttatatta aatcgctgtg
    26641 caatttcttt ggcaggaatt atttttttga ttgcagctga ataagaatag aggttactct
    26701 gttaatcctg ttcaaatttt attcctcctc tgtgacttcc ataaaaagta tatcctatgc
    26761 gttcttaagc tagctgctgt tagtgcttct cattgcaccc tggttataaa agccatactc
    26821 acaaacctct tcaccaaaac tctttacatc tctaatatag aactctatct agcgaacttt
    26881 tagaaaagat ataaaacatc agagtatgga cagttgcgga tgtacttcag aaaagattag
    26941 atgtctaaaa agcttgtagt taaagctttt tagacatcta aatctaggta ctaaaacaat
    27001 tcatccagta aaatataata ttttattttc tccca
//
