LOCUS       NZ_CP062277             1374 bp    DNA     circular CON 16-NOV-2023
DEFINITION  Enterococcus faecium strain VB3338 plasmid pVB3338_P12, complete
            sequence.
ACCESSION   NZ_CP062277
VERSION     NZ_CP062277.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN16287686
            Assembly: GCF_014874615.1
KEYWORDS    RefSeq.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1374)
  AUTHORS   Balaji,V., Karthick,V., Yamuna,B.D. and Dhanalakshmi,S.
  TITLE     Complete genome of vancomycin resistant Enterococcus faecium_VB3338
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1374)
  AUTHORS   Balaji,V., Karthick,V., Yamuna,B.D. and Dhanalakshmi,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-SEP-2020) Clinical Microbiology, Christian Medical
            College, Ida Scudder Road, Vellore, Tamil Nadu 632004, India
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 2020
            Assembly Method :: CANU v. v. 1.7; UNICYCLER HYBRID v. v. 0.4.6
            Assembly Name                     :: VB3338
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 45.0x
            Sequencing Technology :: Illumina HiSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 11/16/2023 05:04:48
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,196
            CDSs (total)                      :: 3,103
            Genes (coding)                    :: 2,981
            CDSs (with protein)               :: 2,981
            Genes (RNA)                       :: 93
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 40 of 122
            Pseudo Genes (incomplete)         :: 74 of 122
            Pseudo Genes (internal stop)      :: 28 of 122
            Pseudo Genes (multiple problems)  :: 17 of 122
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP062277.1.
            Bacteria and source DNA available from Christian Medical College.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1374
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /strain="VB3338"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /plasmid="pVB3338_P12"
                     /geo_loc_name="India"
                     /lat_lon="12.9165 N 79.1325 E"
                     /collection_date="2019"
                     /collected_by="Christian Medical College"
     gene            join(137..1374,1..10)
                     /locus_tag="IG632_RS15855"
                     /old_locus_tag="IG632_15855"
     CDS             join(137..1374,1..10)
                     /locus_tag="IG632_RS15855"
                     /old_locus_tag="IG632_15855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISEfa11 family transposase"
                     /protein_id="WP_182486066.1"
                     /translation="MNDSIKKMLRITEKDLMITEVSYETLQKKKTLVVDAVLSPTPRAC
                     RSCGSTVVDGNGKAIIVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQSY
                     FVQPRHSIANHVRYKIASLLTEKVSLSFIAKSCQVSLTTVIRTLKEFKSYLPKQSKKIL
                     PRVLMVDEFRSHASIEDKMSFICADGETGKLIDVLPTRKLPRLTSYFLGCTNPEEVEFL
                     VTDMNAAYFQLTKRVLPNAKVVIDRFHIVKHMNQAFNELRIREMNELRKAGQKSQAEKL
                     KKNWRFLLKNRANINHYEYKTWKSFRAPKYPFLTEAMMIDRLLEFSPPLKEAYPFFHEL
                     VEAFRDKDPDLFFSLLAELPETLDDSFREKLQNLLTYEEGITNAMIYPYSNGKIEAKNT
                     HIKTMKRVSYGFKSAL"
                     /name="ISL3-like element ISEfa11 family transposase"
ORIGIN
        1 ggctctataa tttatgggac tgatgaatca gaattgattc ttcagcccca ttttcatttt
       61 aggtattaaa aaacatatcg ttctctagta tgattaaatc accacaaaat aaacaactga
      121 gaggacgata cgctatatga atgattctat caaaaaaatg ctgagaataa cagagaaaga
      181 tttgatgatt acagaggtct cttacgagac ccttcagaag aaaaagacgt tggtcgtcga
      241 tgctgttctc tcgcctactc ctcgtgcttg tagaagttgt ggttctactg tggtagatgg
      301 aaacgggaaa gcaattatag tgaaaaatgg aaaaaaggaa acgattgtcc gttttgaaca
      361 atacaatcat atgcctttgg ttatgcgcct aaaaaagcaa cgctatacct gtaaaaactg
      421 tcgcacccat tggacggctc aaagttattt tgtccaaccc agacattcaa tcgcaaatca
      481 tgttagatat aaaattgctt ctttactgac tgaaaaagta tctttatctt ttattgcgaa
      541 aagctgtcag gtatctttga ccaccgttat tcgtacattg aaagagttta aaagctattt
      601 accaaagcaa tctaagaaga tcctccccag agtattgatg gttgacgaat ttcgttcgca
      661 tgcttccata gaagataaga tgagctttat ttgcgcggat ggcgaaacag gaaaattaat
      721 agatgttttg cctacgcgta aattacctcg attaacaagc tatttcttag gttgtaccaa
      781 tccagaagaa gtagaattct tggtgacaga catgaacgcc gcctacttcc agctcaccaa
      841 acgtgttctg ccaaatgcga aagtcgtgat tgatcggttt catattgtca aacacatgaa
      901 tcaagcgttc aatgagttgc gtatccgtga aatgaatgaa cttcgtaaag ccggacagaa
      961 aagccaggca gaaaaactga aaaagaactg gcgctttttg ctaaaaaatc gtgcaaacat
     1021 caaccattat gaatacaaaa catggaaaag tttccgagca ccaaaatacc catttcttac
     1081 tgaagcaatg atgattgatc gattgcttga gttttctccg cccctaaagg aggcgtatcc
     1141 cttttttcat gaattagttg aagcctttcg agacaaagac cctgacttat ttttctcctt
     1201 attggcagaa cttcccgaaa cgttagatga cagctttcgg gaaaagcttc aaaaccttct
     1261 gacctatgaa gaaggcatca ccaacgcaat gatctatcct tattccaatg gaaaaataga
     1321 agcgaagaat acccacataa agacaatgaa acgagtatcc tacggattta aatc
//
