LOCUS       NZ_CP062143             2780 bp    DNA     circular CON 10-JAN-2024
DEFINITION  Klebsiella quasipneumoniae strain 203 19001 plasmid pE, complete
            sequence.
ACCESSION   NZ_CP062143
VERSION     NZ_CP062143.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN15642698
            Assembly: GCF_014843255.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella quasipneumoniae
  ORGANISM  Klebsiella quasipneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 2780)
  AUTHORS   Zhang,H., Wen,H., Guo,Y. and Wang,G.
  TITLE     Complete Genome Sequence of Klebsiella quasipneumoniae sp. 203 19001
            Isolate from activated sludge in Xuzhou, China
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2780)
  AUTHORS   Zhang,H., Wen,H., Guo,Y. and Wang,G.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-SEP-2020) School of Life Science, Jiangsu Normal
            University, Tongshan New District, Xuzhou, Jiangsu 221116, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 27-DEC-2019
            Assembly Method :: SPAdes v. 3.14.1; canu v. 2019.12.27
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100.0x
            Sequencing Technology             :: Illumina HiSeq; PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/10/2024 01:24:37
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,417
            CDSs (total)                      :: 5,295
            Genes (coding)                    :: 5,118
            CDSs (with protein)               :: 5,118
            Genes (RNA)                       :: 122
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 64 of 177
            Pseudo Genes (incomplete)         :: 112 of 177
            Pseudo Genes (internal stop)      :: 33 of 177
            Pseudo Genes (multiple problems)  :: 27 of 177
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP062143.1.
            Bacteria and source DNA available from School of Life Science,
            Jiangsu Normal University.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2780
                     /organism="Klebsiella quasipneumoniae"
                     /mol_type="genomic DNA"
                     /strain="203 19001"
                     /isolation_source="waste water and activated sludge"
                     /db_xref="taxon:1463165"
                     /plasmid="pE"
                     /geo_loc_name="China:Jiangsu"
                     /collection_date="2018-07-01"
     gene            join(2407..2780,1..28)
                     /locus_tag="IG177_RS26920"
                     /old_locus_tag="IG177_26920"
     CDS             join(2407..2780,1..28)
                     /locus_tag="IG177_RS26920"
                     /old_locus_tag="IG177_26920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009310048.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_050886242.1"
                     /translation="MARVSISEAARLTGKSRTTLHRLIKAGDLSTCSGERNAKMVDTSE
                     LLRVFGPFEQPKGEHHYGQVSGQRDTASADQSEQVIRHLKQEIEHLNTLLSSKDSHIDS
                     LKQGMLLLENHQAKLTESPAPWWKFWKKS"
                     /name="helix-turn-helix domain-containing protein"
     gene            790..1470
                     /locus_tag="IG177_RS26925"
                     /old_locus_tag="IG177_26925"
     CDS             790..1470
                     /locus_tag="IG177_RS26925"
                     /old_locus_tag="IG177_26925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001572662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_072042463.1"
                     /translation="MGRKSREKKERHIKDRASPLKVYRFFKEKSHAEALCNGDVWLSTI
                     ETCRRYEDPLQGDPNEAIQTYSSGFISGGSDDSRFVSAAAHAGIHIGPGCSGITIQNCR
                     SFNKMPDAFVLCTTLEFQPENLNDTFGNYCVEISNVEEFFGLITKKLKGLLPIKLGAMG
                     EVRYGSHSFYGYQPPPGPIGFVKNRDKYSSQKEFRFLWVPETFDIQPRLVKCPELSYCC
                     RMIS"
                     /name="hypothetical protein"
     gene            complement(1719..1802)
                     /locus_tag="IG177_RS27340"
                     /old_locus_tag="IG177_26930"
     CDS             complement(1719..1802)
                     /locus_tag="IG177_RS27340"
                     /old_locus_tag="IG177_26930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007772075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_072042462.1"
                     /translation="MHEPPVQSDCCASSGNYRLESNPERHD"
                     /name="replication initiation protein"
     gene            2216..2407
                     /locus_tag="IG177_RS26935"
                     /old_locus_tag="IG177_26935"
     CDS             2216..2407
                     /locus_tag="IG177_RS26935"
                     /old_locus_tag="IG177_26935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_032489493.1"
                     /translation="MTKQQKTALNMAKFIQHQSLLLLEKLNELDLDVEADLCEKLHDDA
                     EHLFRTLSSRLDALQDGN"
                     /name="Rop family plasmid primer RNA-binding protein"
     Typing          complement(1690..2094)
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="92.157"
                     /coverage="99.0"
                     /evalue="1.6700000000000002e-161"
ORIGIN
        1 atggtggaaa ttctggaaaa aatcctgatc ctgccatgct tgagttttcc tctccggcca
       61 tcccggggag ttcctgaacc ttcccaaaaa tcgccagatc tctgaacgct cctcacgact
      121 aaaaataccc acacactacg caaaattaaa attttgcaga tttgtattta atatcagtgg
      181 tttactttcc tgaaatttcg ttttttaaat tttatttaca tagatccgac tctatgacga
      241 ctcgtcatag agtccccggc gagtcgtcat agagtcggta acgactcagg actataaaaa
      301 caatgagtta ataacgaccc gactctagac ccgactcccc gtttggttgc tttttagact
      361 cggcgactct gggtacgact ctagtgaaga gtcgccgggg aatcgtgtgg tgagtcgtcc
      421 ggcgacttgc cataacgtga ttgccggccg tcgacgaaca cgattcctga acgtgctgaa
      481 aaccggtttt ccggctgcgc cggcaacgct tcacggccac gcaataaatt gcgtccccgt
      541 tcaccgtttt cggatgggaa gaacgccgga aacaggactt accatggctg aatggcgtga
      601 attttcgcgc tacgcacttg cgtgcatact catgcatgcc gtaaaaacag agcctgcgcg
      661 tttctggcgg gttttcgggt ggtttgttgc ctgttttacc ggtttcccgt cagaaacgcc
      721 ctgaggccgt tttagcggtg cgggcaagag atgttatggt aaatgaaaat ttaaaaagga
      781 tatttttcaa tgggtaggaa aagtagagag aaaaaagaaa ggcatattaa agatagagcc
      841 agccccctta aagtatatag gttttttaaa gagaagagtc atgctgaggc attgtgcaat
      901 ggcgatgttt ggctgagcac tattgaaacg tgtagacgat acgaagaccc cctacaggga
      961 gatccgaatg aagcaattca aacctatagc tcgggtttta tctcaggggg cagtgatgac
     1021 agccgttttg tctctgctgc agctcatgcc ggaatccata ttggacctgg ctgtagtgga
     1081 ataacgatac aaaattgtcg ctcttttaat aagatgcccg acgcctttgt tttatgtacg
     1141 acactggaat ttcagccaga aaacttgaac gatacctttg gaaattactg tgtggaaata
     1201 tcaaatgttg aagagttttt tggtcttatt actaaaaagt taaaggggtt attacctata
     1261 aaattaggtg ctatggggga ggtcagatat gggagtcatt ctttttatgg ttaccagcct
     1321 ccaccgggac cgattgggtt tgtaaaaaat cgagataaat attcttcgca gaaagaattc
     1381 agatttcttt gggtgccaga aacatttgat attcaaccca ggttagtcaa atgccctgag
     1441 ctttcatatt gttgccgaat gatatcttag atgacatttt ccatagtgta attcttgact
     1501 gagattacgt taccagcgta atctcctttg gaaacaaaaa gaccgcccag caaggcggtc
     1561 cttttgaagg ttcttagagc agcaactctt tgaaccgcgg taactggctt ggaggagcgc
     1621 agtaaccaaa acttgtcctt tcagtttagc cttaaccggc gcataacttc aagactaact
     1681 cctctaaatc aattaccagt ggctgctgcc aatggcgatt agtcgtgtct ttccgggttg
     1741 gactcaagac gatagttacc ggacgaggcg cagcagtcgg actgaacggg gggttcgtgc
     1801 atacagtcca gcttggagcg aactgcctac ccggaactga gtgtcaggcg tggaatgaga
     1861 caaacgcggc cataacagcg gaatgacacc ggtaaaccga aaggcaggaa caggagagcg
     1921 cacgagggag ccaccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
     1981 ccactgattt gagcgtcaga ttccgtgatg cttgtcaggg gggcggagcc tatgaaaaaa
     2041 cggccgcttt gcggcctttc cttcttcccc atttgaccgc tcggtcttct cgagttttta
     2101 tacgcatgct cctgccgctc gccgcagccg aacgaccgga gcgtagcgac tgagtgagcg
     2161 aggaagcgga aaagagactg gttttacact gagcactgac gctctgaggc catttatgac
     2221 gaaacaacag aaaacagcgc tgaacatggc taaattcatc cagcatcagt ctctgctcct
     2281 gctggagaag cttaacgagc ttgatctcga tgtcgaggcc gacctttgtg agaaactcca
     2341 cgatgatgcg gagcatcttt tccgtacact gtcctcgaga ctggatgcat tacaggatgg
     2401 taactgatgg ccagagtgtc tattagtgag gctgcccgac tgacgggtaa gagtagaaca
     2461 acactgcaca ggttaattaa agcaggtgac ctgtccacct gtagcggtga gcgcaatgca
     2521 aaaatggtag atacttcaga gcttttacgt gtctttggtc cctttgagca accgaagggt
     2581 gaacaccatt atggacaggt ctctggacag cgtgatacag cctctgctga ccagagtgaa
     2641 caggtgattc gtcatctgaa acaggagatt gaacatctga acaccttact ttcgtccaag
     2701 gattcgcaca ttgacagcct gaaacagggc atgctccttc ttgaaaatca ccaggcaaaa
     2761 ttaactgaat ccccggcgcc
//
