LOCUS       NZ_CP061916             6754 bp    DNA     circular CON 16-JUN-2023
DEFINITION  Escherichia coli strain SMBL plasmid p2, complete sequence.
ACCESSION   NZ_CP061916
VERSION     NZ_CP061916.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN16231001
            Assembly: GCF_014805805.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6754)
  AUTHORS   Stacey,V.M., Wright,S.A., Lai,G.K., Griffin,S.D. and Leung,F.C.
  TITLE     Complete genome sequencing of a goat Escherichia coli, SMBL-VMS-V2G2
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6754)
  AUTHORS   Stacey,V.M., Wright,S.A., Lai,G.K., Griffin,S.D. and Leung,F.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2020) Shuyuan Molecular Biology Laboratory, The
            Independent Schools Foundation Academy, 1 Kong Sin Wan Road,
            Pokfulam, Hong Kong HK SAR, Hong Kong
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 31-JAN-2020
            Assembly Method                   :: Unicycler v. Jan-2020
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 20.0x
            Sequencing Technology :: Illumina MiniSeq; Nanopore MinIon
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 06/15/2023 02:14:22
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,852
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,584
            CDSs (with protein)               :: 4,584
            Genes (RNA)                       :: 112
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 80 of 156
            Pseudo Genes (incomplete)         :: 68 of 156
            Pseudo Genes (internal stop)      :: 41 of 156
            Pseudo Genes (multiple problems)  :: 32 of 156
            Pseudo Genes (short protein)      :: 2 of 156
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP061916.1.
            Bacteria and source DNA available from FC Leung/S Griffin:  ISF
            Academy.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..6754
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="SMBL"
                     /isolation_source="feces"
                     /host="Goat"
                     /db_xref="taxon:562"
                     /plasmid="p2"
                     /geo_loc_name="Hong Kong"
                     /collection_date="2018-10"
                     /collected_by="SMBL-VMS"
     gene            133..2352
                     /gene="mobQ"
                     /locus_tag="IE857_RS24000"
                     /old_locus_tag="IE857_24000"
     CDS             133..2352
                     /gene="mobQ"
                     /locus_tag="IE857_RS24000"
                     /old_locus_tag="IE857_24000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobQ family relaxase"
                     /protein_id="WP_052986705.1"
                     /translation="MAIYHLSMKIISRKNGYSAVASAAYRSGSVIPDDRTGLIHDYTRK
                     RGVDDAVILTPANAPSWCGDRSVLWNAVEKAEQRRNSQLAREIELAIPREISREAARET
                     VLAFVRENFVSRGMIADVAFHHMDRTNPHAHIMLTTRAVGETGFAGKVRDWNDRALAET
                     WRASWADHANRALANAGYQEEIDHRSYERQGLEKAPGLHLGKAACAMEKRGMETERGEQ
                     NRLINSLNLEIQVSRTQLALRTVQEAQRKRELSDAARRAAEALNLTIPAANASADTLRE
                     FIATLPQECGNAWEMTPEFLAMSGKVNDIEREGNALLKEQAILEKEMTGLKKARPVASL
                     LSEIPLMTWAEPEYRKRQLRFWKLGKQIESLRRTYRAVKERDIPARRQAFETQWNTWIA
                     PGMAELKEKLSAREAERRREEAEAEARRKEQEHEARLKRHDNHRLSRETALVGVITELG
                     RAREPGTGRITRYMMLSNRAGEFTVWGDELAHYPQSVHDPVNVYLSPGGAVMVSDIREG
                     MPESHETMVRPERVRMYSGATVRHVLEQMRQGWPSYGFPALPHHWPDNFYFSDDRRPVA
                     SPLPSAHRVDITADAVPEQLMPVVFSREGNSRTLNLLLCKGPEEMLVGFVRREGGFCPV
                     LVLPSPDYSHLIVSTITENGVCLAGYGEAINHDEDTPYPPEPHLMQFRLKGHHDRLLAA
                     VHKPEEMPDYLFRQLGFNQTWHEWKRDEQHRQQQRRPGHFRGMSM"
                     /name="mobQ"
     gene            2585..4330
                     /gene="col"
                     /locus_tag="IE857_RS24005"
                     /old_locus_tag="IE857_24005"
     CDS             2585..4330
                     /gene="col"
                     /locus_tag="IE857_RS24005"
                     /old_locus_tag="IE857_24005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_172690082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-E2"
                     /protein_id="WP_052986704.1"
                     /translation="MSGGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNP
                     WGGGSGSGIHWGGGSGHGNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLA
                     VSISAGALSAAIADMMAALKGPFKFGLWGVALYGVLPSQIAKDDPNMMSKIVTSLPADD
                     ITESPVSSLPLDKATVNVNVRVVDDVKDERQNISVVSGVPMSVPVVDAKPTERPGVFTA
                     SIPGAPVLNISVNNSTPAVQTLSPGVTNNTDKDVRPAGFTQGGNTRDAVIRFPKDSGHN
                     AVYVSVSDVLSPDQVKQRQDEENRRQQEWDATHPVEAAERNYERARAELNQANEDVARN
                     QERQAKAVQVYNSRKSELDAANKTLADAIAEIKQFDRFAHDPMSGGHRMWQMAGLKAQR
                     AQTDVNNKQAAFDAAAKEKSDADAALSAAQERRKQKENKEKDAKDKLDKESKRNKPGKA
                     TGKGKPVGDKWLDDAGKDSGAPIPDRIADKLRDKEFKNFDDFRRKFWEEVSKDPELSKQ
                     FNPGNKKRLSQGLAPRARNKDTVGGRRSFELHHDKPISQDGGVYDMDNLRITTPKRHID
                     IHRGQ"
                     /name="col"
     gene            4333..4590
                     /locus_tag="IE857_RS24010"
                     /old_locus_tag="IE857_24010"
     CDS             4333..4590
                     /locus_tag="IE857_RS24010"
                     /old_locus_tag="IE857_24010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000421100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="WP_000421100.1"
                     /translation="MELKNSISDYTETEFKKIIEDIINCEGDEKKQDDNLEHFISVTEH
                     PSGSDLIYYPEGNNDGSPEAVIKEIKEWRAANGKSGFKQG"
                     /name="bacteriocin immunity protein"
     gene            4652..4795
                     /locus_tag="IE857_RS24015"
                     /old_locus_tag="IE857_24015"
     CDS             4652..4795
                     /locus_tag="IE857_RS24015"
                     /old_locus_tag="IE857_24015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000724569.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_001314446.1"
                     /translation="MKKITGIILLLLAVIILAACQANYIRDVQGGTVSPSSTAEVTGLA
                     TQ"
                     /name="colicin release lysis protein"
     gene            5324..6217
                     /locus_tag="IE857_RS24020"
                     /old_locus_tag="IE857_24020"
     CDS             5324..6217
                     /locus_tag="IE857_RS24020"
                     /old_locus_tag="IE857_24020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_001277133.1"
                     /translation="MSAVLQRFREKLPHKPYCTNDFAYGVRILPKNIAILARFIQQNQP
                     HALYWLPFDVDRTGASIDWSDRNCPAPNITVKNPRNGHAHLLYALALPVRTAPDASASA
                     LRYAAAIERALCEKLGADVNYSGLICKNPCHPEWQEVEWREEPYTLDELADYLDLSASA
                     RRSVDKNYGLGRNYHLFEKVRKWAYRAIRQGWPVFSQWLDAVIQRVEMYNASLPVPLSP
                     AECRAIGKSIAKYTHRKFSPEGFSAVQAARGRKGGTKSKRAAVPTSARSLKPWEALGIS
                     RATYYRKLKCDPDLAK"
                     /name="replication initiation protein"
     gene            complement(6433..6708)
                     /locus_tag="IE857_RS24025"
                     /old_locus_tag="IE857_24025"
     CDS             complement(6433..6708)
                     /locus_tag="IE857_RS24025"
                     /old_locus_tag="IE857_24025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000091752.1"
                     /translation="MSRTLEQKIAEAEARLQRLKAKSRSLDTAQKVIVGAAMLARVRRP
                     EEAQLRAFLLQFLRKEVTRQADVNRLQPLINELEKLPRPPAKPQNH"
                     /name="hypothetical protein"
     BGC             1..6754
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          complement(2..132)
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="2.05e-62"
     Typing          complement(133..491)
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="99.167"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          5325..6217
                     /name="rep_cluster_2150"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="91.051"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          134..2349
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.865"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 ggtagttgca aaactgaact ggcataatgc acggcacatc acgaagtgcg cacttataca
       61 atctccactt cgtttcgatt gtgtgcggtc tgcgacgcta aaagaaaacg gcaaaaaggc
      121 attacggcag aaatggcgat ttatcatctc agcatgaaaa tcatttcgcg aaaaaacggc
      181 tacagtgctg ttgcttctgc tgcctaccgt tccggctctg tcatacccga tgaccgtacc
      241 ggattaatcc acgattacac ccgtaaacgc ggcgttgatg atgcggtcat tctcacccct
      301 gcgaatgcac cgtcctggtg tggtgaccgt tccgttctct ggaatgcggt cgagaaagcc
      361 gaacagcgcc ggaactccca gctggcaagg gagattgaac ttgccattcc ccgtgagatt
      421 tcccgcgagg ccgcacggga gaccgttctc gcttttgtcc gggaaaactt tgtcagtcgg
      481 ggcatgattg ccgatgtggc gttccatcac atggaccgga ccaatcccca tgcgcacatc
      541 atgctgacca cgagagctgt cggggaaacg ggattcgcag gaaaggtcag ggactggaac
      601 gaccgggcac tcgccgagac gtggcgcgca tcatgggctg accatgcgaa cagagcgctt
      661 gcgaacgccg gctaccagga agagatagac catcgttcat acgagcgtca gggactggag
      721 aaagcgccgg gccttcacct cggaaaggct gcctgtgcga tggaaaaacg gggaatggaa
      781 acagaacgcg gtgagcagaa ccgtctgatt aacagcctta acctggaaat acaggtttcc
      841 cgcacgcagc ttgctctcag gacggttcag gaagcgcagc gtaagcggga actcagcgat
      901 gctgcacgtc gtgcagcgga agcccttaac ctgaccattc ccgctgcgaa tgcctcagcg
      961 gataccctgc gggaattcat tgccacgctg ccgcaggaat gcgggaacgc gtgggagatg
     1021 accccggagt tcctggcgat gagcgggaag gtgaacgaca tcgaacgtga ggggaatgcg
     1081 ctgctgaaag agcaggccat tctcgaaaag gagatgaccg gactcaaaaa agcacgccct
     1141 gtcgcgtccc ttctgtcaga gattcccctg atgacatggg ctgaaccgga ataccgcaaa
     1201 agacaactcc gtttctggaa actcgggaaa cagattgaat ctcttcgccg cacctacagg
     1261 gccgtgaaag aacgggacat tcccgcccgt cgtcaggcct ttgaaacgca gtggaatacg
     1321 tggattgcgc cgggaatggc agagctgaaa gaaaaactgt cagcacggga agcggagcgg
     1381 cgcagggagg aagccgaagc ggaagcgcgc cggaaggaac aggagcatga ggcgcggctg
     1441 aaacgtcatg ataaccaccg tctgagccgt gaaacggcat tagtcggggt tattacggag
     1501 ctggggcgtg ccagagagcc gggaacgggc aggataaccc gctacatgat gctgagtaac
     1561 agagccggag aattcacggt atggggtgat gagctggcgc attaccccca gagtgttcat
     1621 gacccggtga atgtttacct gtcgccaggc ggggctgtga tggtctcgga tatacgcgag
     1681 ggaatgccag aatctcatga gacgatggtg cggcctgagc gtgtgagaat gtattccggt
     1741 gcgacggtcc ggcatgtact ggaacagatg cgccaggggt ggccctctta cggttttccg
     1801 gcgctgccgc atcactggcc ggataatttt tatttcagcg acgaccgcag gcccgtagcc
     1861 tctccgctgc cgtcagcgca ccgggtggat atcaccgctg atgctgtacc ggaacaactc
     1921 atgcctgttg tattttcgag agagggaaac agcaggacgc tgaatctgtt gttatgcaaa
     1981 gggccggagg aaatgcttgt cgggtttgtt cgacgggaag gagggttttg ccccgttctt
     2041 gtgcttccgt cgccggatta cagtcatctg atagtcagca ccatcacgga gaacggggta
     2101 tgcctggcag gttacggaga agctataaac catgatgagg atactccgta cccaccggaa
     2161 ccgcacctga tgcagttccg gctcaaaggc catcatgaca ggcttttggc tgctgtccac
     2221 aaaccggaag agatgccgga ttatctcttc cgtcaactcg gttttaatca gacctggcat
     2281 gagtggaagc gggacgaaca gcacaggcaa caacaacgcc gccccgggca cttccggggc
     2341 atgagtatgt gatatccggg gctgcacccc ggaccccgcc aacacatcac gggccacaaa
     2401 attttttgtg gcccgctctg cgttttctaa gtgttatccc tcctgatttc taaaaaattt
     2461 tccacctgaa cttgacagaa aaaacgatga cgggtacttt ttgatctgta cataaaacca
     2521 gtggttttat gtacagtatt aatcatgtaa ttaattgttt taacgcttaa aagagggagt
     2581 ttttatgagc ggtggcgatg gacgcggcca taacacgggc gcgcatagca caagtggtaa
     2641 cattaatggt ggcccgaccg ggcttggtgt aggtggtggt gcttctgatg gttccgggtg
     2701 gagttcggaa aataacccgt ggggtggtgg ttccggtagc ggcattcact ggggtggtgg
     2761 ttccggtcat ggtaatggcg gggggaatgg taattccggt ggtggctcgg gaacaggcgg
     2821 taatctgtca gcagtagctg cgccagtggc atttggtttt ccggcacttt ccactccagg
     2881 agctggcggt ctggcggtca gtatttcagc gggagcatta tcggcagcta ttgctgatat
     2941 gatggctgcc ctgaaaggac cgtttaaatt tggtctttgg ggggtggctt tatatggtgt
     3001 attgccatca caaatagcga aagatgaccc caatatgatg tcaaagattg tgacgtcatt
     3061 acccgcagat gatattactg aatcacctgt cagttcatta cctctcgata aggcaacagt
     3121 aaacgtaaat gttcgtgttg ttgatgatgt aaaagacgaa cgacagaata tttcggttgt
     3181 ttcaggtgtt ccgatgagtg ttccggtggt tgatgcaaaa cctaccgaac gtccaggtgt
     3241 ttttacggca tcaattccag gtgcacctgt tctgaatatt tcagttaata acagtacgcc
     3301 agcagtacag acattaagcc caggtgttac aaataatact gataaggatg ttcgcccggc
     3361 aggatttact caggggggta ataccaggga tgcagttatt cgattcccga aggacagcgg
     3421 tcataatgcc gtatatgttt cagtgagtga tgttcttagc cctgaccagg taaaacaacg
     3481 tcaagatgaa gaaaatcgcc gtcagcagga atgggatgct acgcatccgg ttgaagcggc
     3541 tgagcgaaat tatgaacgcg cgcgtgcaga gctgaatcag gcaaatgaag atgttgccag
     3601 aaatcaggag cgacaggcta aagctgttca ggtttataat tcgcgtaaaa gcgaacttga
     3661 tgcagcgaat aagactcttg ctgatgcaat agctgaaata aaacaatttg atcgatttgc
     3721 ccatgaccca atgtctggcg gtcacagaat gtggcaaatg gccgggctta aagcccagcg
     3781 ggcgcagacg gatgtaaata ataagcaggc tgcatttgat gctgctgcaa aagagaagtc
     3841 agatgctgat gctgcattaa gtgccgcgca ggagcgccgc aaacagaagg aaaataaaga
     3901 aaaggacgct aaggataaat tagataagga gagtaaacgg aataagccag ggaaggcgac
     3961 aggtaaaggt aaaccagttg gtgataaatg gctggatgat gcaggtaaag attcaggagc
     4021 gccaattcca gatcgcattg ctgataagtt gcgtgataaa gaatttaaaa acttcgacga
     4081 ttttcggaga aaattctggg aagaagtgtc gaaagatcct gagttaagta agcaatttaa
     4141 tccaggtaat aaaaaacgct taagccaagg attagctcca cgggctagga ataaagatac
     4201 tgtcggtggg cgtcgctcat ttgagcttca tcacgacaag ccaatcagtc aggatggtgg
     4261 tgtttatgat atggacaacc tccgtatcac tacgccgaaa cgccatattg atattcacag
     4321 aggtcagtaa aaatggaact gaaaaacagc attagtgatt acactgaaac tgaattcaaa
     4381 aaaattattg aagacatcat caattgtgaa ggtgatgaaa aaaaacagga tgataacctc
     4441 gagcatttta taagtgttac tgagcatcct agtggttctg atctgattta ttacccagaa
     4501 ggtaataatg atggtagccc tgaagctgtt attaaagaga ttaaagaatg gcgagctgct
     4561 aacggtaagt caggatttaa acagggctga aatatgaatg ccagttgttt aaggatgaat
     4621 gactggcatt ctttcacaac aaggagtcgt tatgaaaaaa ataacaggga ttattttatt
     4681 gcttcttgca gtcattattc tggctgcatg tcaggcaaac tatatccggg atgttcaggg
     4741 cgggaccgta tctccgtcat caacagctga agtgaccgga ttagcaacgc agtaacccga
     4801 aatcctcttt gacaaaaaca aagcgtgtca ggctgattct gacgcgcttt tttttgaagt
     4861 gtcacaaaaa ttccatgtgg gagatgggat ctaaaatccc cgtgcagaac tttccatcca
     4921 gggggagaaa acttgtcgtt ttgagccgtt cggtgttcag aacgcacgaa accgatcgtg
     4981 catcgctttc gtgcatagtt atgcaacgcg ctaaaaacga ttctgacgca ttttttatgt
     5041 ttaccctggt gtttccttgt ctttttgttt tttttgcgtc agaaagcgat tgagggtgtt
     5101 ttaaggggta cgtacaacgg gagttatggt aaatggatcg gtttttcggg aaggatcgac
     5161 aggatttgcc gttgggtgta gtgtaagcga ctgaaaaaca aacgccccgt aaatcgtgct
     5221 ctcaccgcca agattgatca cgaaattaca gggcgccggg ttccgcgttt cccgatggga
     5281 aagcgcggtt agttaaactg tgtaccgaga gaaatcgtat cacatgagcg ccgtacttca
     5341 acgcttcagg gaaaaattac cgcacaaacc gtactgtacg aacgatttcg cgtacggcgt
     5401 tcgcattctg ccgaaaaaca ttgccattct tgcccgtttc atccagcaga accagccaca
     5461 tgcactgtac tggcttccct ttgacgtgga ccggacaggg gcatcaatcg actggagcga
     5521 ccggaattgt ccggccccta acatcaccgt aaaaaatccc cgtaacgggc acgcgcatct
     5581 gctctacgcg ctcgcccttc ctgtgagaac tgcgccggat gcatcggctt cggcgctcag
     5641 atacgctgcc gctattgagc gtgcgttgtg tgaaaaactg ggcgcggatg tgaattacag
     5701 cggcctgatc tgcaaaaatc cgtgccaccc tgaatggcag gaagtggaat ggcgcgagga
     5761 accctacact ctcgacgaac tggctgatta tctcgatttg agcgcctcag cgcgccgtag
     5821 cgtcgataaa aattacgggc tggggcgaaa ctaccatctg ttcgaaaagg tccgtaaatg
     5881 ggcctacaga gcgattcgtc agggctggcc tgtattctca caatggcttg atgccgtgat
     5941 ccagcgtgtc gaaatgtaca acgcatcgct tcccgttccg ctttctccgg ctgaatgtcg
     6001 ggctattggc aagagcattg cgaaatatac acacaggaaa ttctcaccag agggattttc
     6061 cgctgtacag gccgctcgag gtcgcaaggg tggaactaaa tctaagcgcg cagcagttcc
     6121 tacatcagca cgttcgctga agccgtggga ggcattaggg atcagtcgag cgacgtacta
     6181 ccgaaaatta aaatgtgatc cagatctcgc aaaatgagac cagataagcc ttatcagata
     6241 acagcgccct tttggcgtct ttttgagcag tgatctttct tgcgatttcc tccgttctgc
     6301 cccgtaatgg cgtggctttc cgcatgattg aggtggtagc gctcgccgca gtctcatgac
     6361 cgagcgtagc gagcgaatga gcgaggaagc gcaaaggcgc ccggtggtgc atgggcactt
     6421 acgcgccggg gcttagtggt tctgcggttt cgccggtggt ctgggtagct tctccagctc
     6481 gttaatcagt ggttgtagtc ggttcacatc cgcctgtctt gtgacttcct ttcgcagaaa
     6541 ctggagcagg aacgcacgca gttgcgcttc ttcaggcctc cgtactctcg ccagcatggc
     6601 agcccccaca atgacttttt gcgccgtgtc caggctccgg ctcttcgcct tcaggcgctg
     6661 taatctggcc tcagcttcgg caatcttctg ttcgagtgtt ctgctcattt cgtgactccg
     6721 tgcgcggtga aaaatcgcat tttagcgcgt cact
//
