LOCUS       NZ_CP061831             3857 bp    DNA     circular CON 10-JAN-2024
DEFINITION  Acinetobacter seifertii strain AS42 plasmid pAS42-3, complete
            sequence.
ACCESSION   NZ_CP061831
VERSION     NZ_CP061831.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN16092226
            Assembly: GCF_014788525.1
KEYWORDS    RefSeq.
SOURCE      Acinetobacter seifertii
  ORGANISM  Acinetobacter seifertii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Acinetobacter; Acinetobacter calcoaceticus/baumannii
            complex.
REFERENCE   1  (bases 1 to 3857)
  AUTHORS   Li,L.-H., Yang,Y.-S., Sun,J.-R., Huang,T.-W., Huang,W.-C.,
            Wang,Y.-C., Kuo,T.-H., Kuo,S.-C. and Chen,T.-L.
  TITLE     Clinical and molecular characterization of Acinetobacter seifertii
            in Taiwan
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3857)
  AUTHORS   Chen,F.-J. and Lee,Y.-T.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2020) Department of Emergency Medicine, Taipei
            Veterans General Hospital, No.201, Section 2, Shipai Road, Taipei
            112, Taiwan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 2020
            Assembly Method                   :: CCBGpipe v. 1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 80x
            Sequencing Technology             :: Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/10/2024 01:21:17
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,254
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,004
            CDSs (with protein)               :: 4,004
            Genes (RNA)                       :: 97
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 79 of 153
            Pseudo Genes (incomplete)         :: 70 of 153
            Pseudo Genes (internal stop)      :: 25 of 153
            Pseudo Genes (multiple problems)  :: 17 of 153
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP061831.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3857
                     /organism="Acinetobacter seifertii"
                     /mol_type="genomic DNA"
                     /strain="AS42"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1530123"
                     /plasmid="pAS42-3"
                     /geo_loc_name="Taiwan"
                     /collection_date="2010/2017"
                     /collected_by="TVGH"
     gene            join(3714..3857,1..303)
                     /locus_tag="IC779_RS21120"
                     /old_locus_tag="IC779_21120"
     CDS             join(3714..3857,1..303)
                     /locus_tag="IC779_RS21120"
                     /old_locus_tag="IC779_21120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017482451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_308401008.1"
                     /translation="MKRKDTWRPLCYADVFHDLVQGQAPETPKSKGKSMTAIKRNKVYK
                     VRFSDAEVQKIKDANILNIAKYLRDTSLAQIDATQGNAKKKNQENHYTKLDRDFLLELS
                     RIGGNINQIAKAINTDIASSRPLDAARLLHLLIGIDQTLKELKK"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            300..2201
                     /locus_tag="IC779_RS21125"
                     /old_locus_tag="IC779_21125"
     CDS             300..2201
                     /locus_tag="IC779_RS21125"
                     /old_locus_tag="IC779_21125"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="WP_144624569.1"
                     /translation="MIVQFFRYGNGLSKGPLDYLLGKDRDRDYAKVLQGDVSEVSGLID
                     TSPYAKKYTSGCLSFYEHDLSDQDKQKIMQDFEQALFPGMDQSQYRVLWIEHQDKLNEE
                     TGERRLELNFLIPNVEILTGQRLQPYYDKADRPRIDLFKKITNYEYQLHDADDPLYRQA
                     VTTAKNLPKTVNEIKETLDIEATRAVEGGLITDRQSMKKWLLDLGLEVTRETKKSLSIK
                     NPNDDEKARPIRLTGAIYEQDFRLTETSQQLTIAASERYRREAKQRNQSDIQRYAEYSA
                     KRSTELEQQYRQHQNVYTAETERSNERDIAADRPDYSSNQEAAATRHDSPIARTTTADQ
                     TATSELAAVEQLEPRNSPSSQIKENPYYIEYSLDFTGMYNAYQQYLSGVRQQEQIQRHH
                     RDGEQSPVVKKPRGEPEYSEVWDREKMELVRPDRPESAAVQQQHANSAGNQLNESRSTV
                     IADYRAATAAAQRATEAARASLAAHSGTEQNHRRIRELQQQTSEALQPVERERKRADQY
                     HQETTESEAIRGFIARLGEQLKTAITEPFRAISDWVKSKERDQRTIANDDLYRNREADS
                     TIERTASKETRLSATVSRKIRGFDQTNIFKALDELDRRRELQLEQQRKNDRSYSPRF"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            2487..3449
                     /locus_tag="IC779_RS21135"
                     /old_locus_tag="IC779_21135"
     CDS             2487..3449
                     /locus_tag="IC779_RS21135"
                     /old_locus_tag="IC779_21135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004977197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_071212525.1"
                     /translation="MLNKTQFSDDFYEKLPKKPYCSDDLGRGVIIRPKRTAIQKPYIQH
                     NPPCLVSSLVFDIDRQDAYFAWSDANLPTPTWIAKNRQNGHAHIGYMLLAPVCTTHRAK
                     QNVIQYLAKIEQAYSLALGADRGYTGLITKNPCHGTWENHTFGVQPYDLNYLADFVELQ
                     ELKTDLKEVSGLGRNCMMFDTVRFWAYEAIRAFISGSPDSWYAEVLKVAINANGAFLEP
                     LPYSEVKATAKSIARWVWRNHTEAHAKFIERQTVKGRKGGLSSDSSHGGKARSQQYSDM
                     RQEALKLHIQGIKQKDIALQLNVSDRTIRTWIKKRKVQA"
                     /name="replication initiation protein"
     Typing          301..2195
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="92.575"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 tctgatgcag aggtacagaa aatcaaagat gccaatatcc taaatattgc caaatatcta
       61 agggatactt cgcttgccca aattgatgca acccaaggca acgccaaaaa aaagaatcag
      121 gaaaaccact acacaaaatt agaccgtgac tttttgcttg agctatcccg aattgggggc
      181 aacatcaatc agattgcaaa ggcaataaat actgatattg caagcagtcg tccccttgat
      241 gctgcaaggc ttttgcacct gttaataggg attgatcaga ccttaaagga attgaaaaaa
      301 tgattgttca attctttaga tatgggaacg gtttaagtaa aggtccactg gattatttac
      361 ttggtaaaga ccgtgatcga gattatgcaa aagtcctaca gggcgatgtt tccgaagtat
      421 caggattaat tgatacgtca ccttatgcaa aaaaatatac atccggctgc ctaagttttt
      481 atgagcatga tttatctgac caggataaac aaaaaatcat gcaggatttt gagcaagcct
      541 tattccctgg catggatcag agccagtacc gtgtcttgtg gattgagcat caagacaaat
      601 taaacgaaga aacaggggag cgaagattag agcttaactt cctaatccct aatgttgaaa
      661 tccttacagg gcaacgttta cagccttatt atgataaagc tgatcgtcct cgtattgatc
      721 tgtttaagaa gattacaaac tatgaatatc aactgcatga tgcagatgac cccctctatc
      781 gccaagccgt tacaacggca aaaaacttgc ctaaaactgt taatgaaatc aaggaaacgc
      841 ttgatattga agcaacgaga gccgttgaag gtggactaat tacagatcgg cagagcatga
      901 aaaaatggct attggatctt ggtcttgaag tcaccagaga aaccaagaaa agcctatcca
      961 ttaaaaatcc gaatgatgac gaaaaagccc gcccgataag gcttacaggt gcaatctatg
     1021 agcaagattt tagacttaca gaaacgagcc aacaacttac tatcgcagca tcagaacgat
     1081 atagacgaga agctaaacag cgaaatcagt cagatattca aagatatgcg gaatacagtg
     1141 caaagcgaag cacagagctt gagcagcagt atagacagca ccaaaacgta tatacagcag
     1201 aaacagagcg aagcaacgaa cgagatattg cagcagatcg accagactac agctcaaacc
     1261 aagaagcagc agcaacaagg catgacagcc ctattgcaag aacaacaaca gcagatcaaa
     1321 cagctacaag cgaacttgca gcagttgaac agcttgagcc aagaaacagc ccaagcagcc
     1381 aaattaaaga aaacccttat tacatcgaat atagccttga tttcactggc atgtataacg
     1441 cttatcagca gtatttgtct ggggtacgtc agcaagagca aatacagcga catcacagag
     1501 atggagaaca aagtccagtg gttaaaaagc caaggggcga gcctgaatat tcagaagtgt
     1561 gggacagaga aaaaatggaa ctggtgcgtc cagatcgacc cgaaagcgca gcagtacagc
     1621 aacaacatgc gaatagtgca ggaaaccaat taaatgaatc tcgaagcaca gttattgcag
     1681 attatcgagc agcaacagca gcagctcaaa gagcaacaga agcagcaaga gcaagccttg
     1741 cagctcattc aggcacagaa cagaaccata gacgaattag agaactacaa cagcaaacta
     1801 gtgaagcact gcaaccagtt gaacgagaaa gaaaacgagc tgatcaatat catcaagaaa
     1861 ctacagaatc cgaagctatc agaggattta ttgcaaggct tggagagcag cttaaaacag
     1921 cgattacaga gccatttaga gcaattagtg actgggttaa atctaaagaa cgagatcaac
     1981 gaactattgc caacgatgat ctatacagaa atcgagaagc agacagcacc attgaacgaa
     2041 cagcttcaaa ggaaactaga ctcagtgcaa cagtatcacg aaaaattcga gggtttgatc
     2101 aaacaaatat cttcaaggct ttagatgaac tggacagacg tagagagttg caactggagc
     2161 aacaacgtaa aaatgacagg agttattcac ctagattttg atactttttt ttctgcggag
     2221 cattgccctt gcaatgctgt cctcgaaaaa aagcatcttc aaaggtcaca tatttgcgac
     2281 attttgtcgc aatattttgt tttgaaaatc agtcatttga cagcaattca aaaaggtgta
     2341 atattttcat caggcgaaaa aaaagcaggt ttggcgaccc gcttttttct ctttgttaaa
     2401 caaacgctta aagcatccgt aaaactcaaa ttcgatactt gtattttact gatgttttga
     2461 gccaatgcaa cacttggacg tgtttcatgc tcaataaaac gcaattttca gacgattttt
     2521 acgaaaaact cccaaaaaaa ccgtattgct cggatgattt agggcgtggg gtgattattc
     2581 gcccaaaacg taccgcaatc cagaagccat acattcagca taatccccct tgtcttgtta
     2641 gctcgttggt gttcgatata gaccgccaag acgcttattt tgcgtggtct gacgcaaatt
     2701 taccgacccc aacatggata gccaaaaatc gccaaaatgg acatgcacat attggctata
     2761 tgcttttagc tccagtgtgt accacgcatc gagcaaagca gaatgtcata cagtaccttg
     2821 ccaagattga gcaggcgtat agcttggcgt taggtgcaga tcgtggctat acggggttaa
     2881 tcactaaaaa tccatgtcac ggaacatggg aaaatcatac ctttggcgtt caaccatacg
     2941 acctaaatta tttagccgat tttgtagagc ttcaggaact aaaaaccgac ttaaaagaag
     3001 tttcagggct tgggcgtaac tgtatgatgt tcgataccgt tcgtttttgg gcgtatgaag
     3061 cgattagagc gtttatttcg gggtcgcctg atagttggta tgcggaagtg ctaaaagtcg
     3121 ctataaacgc aaatggtgcg tttttagagc cattgccata ttcagaggta aaggcaaccg
     3181 ctaagtctat tgctcgttgg gtatggcgca atcacacaga agcacatgcc aaatttatcg
     3241 agcgtcagac tgtcaaaggg cgtaaaggcg gtctaagcag tgattccagt catggcggta
     3301 aggctaggtc acagcaatac agtgatatgc gtcaggaagc cttaaaactg catatacagg
     3361 gcataaagca gaaagatata gccctacaat tgaatgtttc ggatagaaca atcagaacgt
     3421 ggataaaaaa gcggaaagtg caagcctaaa tcagatatca gccccctagg gtgctttttt
     3481 cccatttcat ttttaaaaat tattattaaa gggaatgagc aacggctgtg ggcaaagtaa
     3541 aaccctgtga tctaaacctg tgtgcaactc aacgtataac ggctttatcg ttatgcggag
     3601 ttgtgcatag tgtttaggca cagatcaggt tttacggtcg cttgcgattg tccatagtct
     3661 tgctatgacc tgcttaatct taattgaatt acgtctaggg gtctgctatc gtaatgaaac
     3721 gcaaggatac atggagaccc ctctgctacg cagatgtgtt ccatgacctt gttcaggggc
     3781 aagcccccga aacccccaaa agcaaaggca aaagcatgac agcgatcaaa cgtaataagg
     3841 tttacaaagt ccgattt
//
