LOCUS       NZ_CP061827             1928 bp    DNA     circular CON 07-NOV-2023
DEFINITION  Enterococcus faecium strain A6521 plasmid pA6521_10, complete
            sequence.
ACCESSION   NZ_CP061827
VERSION     NZ_CP061827.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN14675386
            Assembly: GCF_012933195.2
KEYWORDS    RefSeq.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1928)
  AUTHORS   Veeraraghavan,B., Bakthavatchalam,Y.D., Vasudevan,K. and
            Solaimalai,D.
  TITLE     Complete genome of vancomycin resistant Enterococcus faecium_A6521
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1928)
  AUTHORS   Veeraraghavan,B., Bakthavatchalam,Y.D., Vasudevan,K. and
            Solaimalai,D.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-SEP-2020) Clinical Microbiology, Christian Medical
            College, Ida Scudder Road, Vellore, Tamil Nadu 632004, India
REFERENCE   3  (bases 1 to 1928)
  AUTHORS   Veeraraghavan,B., Solaimalai,D., Vasudevan,K. and Arumugam,A.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-APR-2020) Department of Clinical Microbiology,
            Christian Medical College and Hospital, Ida Scudder Road, Vellore,
            Tamil Nadu 632004, India
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 2020
            Assembly Method :: CANU v. v. 1.7; UNICYCLER HYBRID v. v. 0.4.6
            Assembly Name                     :: A6521
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 62.0x
            Sequencing Technology :: Illumina HiSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 11/07/2023 09:17:18
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,203
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,007
            CDSs (with protein)               :: 3,007
            Genes (RNA)                       :: 92
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 40 of 104
            Pseudo Genes (incomplete)         :: 63 of 104
            Pseudo Genes (internal stop)      :: 28 of 104
            Pseudo Genes (multiple problems)  :: 21 of 104
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP061827.1.
            Bacteria and source DNA available from Christian Medical College.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1928
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /strain="A6521"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /plasmid="pA6521_10"
                     /geo_loc_name="India:Vellore"
                     /lat_lon="12.9165 N 79.1325 E"
                     /collection_date="2019"
                     /collected_by="CMC Vellore"
     gene            complement(join(1184..1928,1..221))
                     /locus_tag="HI837_RS15890"
                     /old_locus_tag="HI837_015890"
     CDS             complement(join(1184..1928,1..221))
                     /locus_tag="HI837_RS15890"
                     /old_locus_tag="HI837_015890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305967.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0003916 - DNA topoisomerase activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="WP_191664883.1"
                     /translation="MSKKGIIVNEWEKLNRISNRRLNVQNLNTPLKLQWSIDRITIVGK
                     LKENIYYHTPNDVLILNFEQLMQLNEGNGFVKSVGKNGWQIVDQYEENIAYIELLQFHE
                     GKARIDFNPNKIGQFLASSMKNFIHDLFLEPHFSRADIACDMVNVPDEFIMQYRVVDPV
                     SFKPIYGRSGKLESAYWGSRASERQIRLYNKKLEQETKRKVVPKEVETWWRLEMQLRHG
                     KATDWHNMVQESLNSFASLHFLPIDTTPTDRIMLAGLMSDQNFWSLLSRNSKYKYRELL
                     KEESQNDELTNHLRETFAESADNLKQELDTWLLGLDVTEK"
                     /name="replication initiation factor domain-containing
                     protein"
     gene            complement(<802..984)
                     /locus_tag="HI837_RS15895"
                     /old_locus_tag="HI837_015895"
                     /pseudo=""
     CDS             complement(<802..984)
                     /locus_tag="HI837_RS15895"
                     /old_locus_tag="HI837_015895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313295.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /name="hypothetical protein"
ORIGIN
        1 ccattcccct cattcaattg catcaattgc tcaaaattta aaatcaagac atcatttggt
       61 gtgtgataat aaatattttc tttcaatttt ccaacaatgg taattcggtc gattgaccac
      121 tgtaatttta ggggggtgtt caaattttga acatttaacc gcctgttaga aatacggtta
      181 agtttttccc actcatttac gattatgcct tttttactca aaaatagctc ctcctcaatg
      241 aaaaaagaga ttcttggaaa gaatttccag agttcacgta actctaaaaa tccacatcca
      301 aaaatctcta gcttgaagaa acctaaaaat attcgtataa tgaacttgag ttacggaact
      361 aggtgaagta gtttccgtaa aggtcgagag tgcgtcaaca ctgctcgact atttttttac
      421 tcttttttct cattttcttt gattatagca ctcctctaga aaaaataaaa agacctcagg
      481 gaatttcatt ccccgaaccc cttgagcctc ctcctgccga aatagcatgg cgctgccagc
      541 atttatttcg ttcagctatc ggctacacaa tctaacacga ccgttccgct accagtcgcc
      601 ttacaggctc cattttcgct cctctaacgt gtttcaccga ttgtgccgat aactaaacga
      661 aataaacgct aaaaccgctc agaacgcaaa atatgccatt ttagagttat tgtagaaaat
      721 gtagtttgaa atgcaatttt cttctttcag ctttttattt cccacttgta aatctgctaa
      781 ttattttcta ccagacaaac cattgatttt ttggctatca gttaactcac gattattatg
      841 agaaatgctt gtattctttg ttcccttttt gaacgaaata ctaaacccca agttagaccc
      901 ctccttcgcg ctgtcgctaa taaaaagagt gtaaaccttt ctgttactct ttgacaaaga
      961 gtaacatttt cctaattgtg acatggctgt cacaattcca aatgctctcc gagggtattc
     1021 gctgtcacaa gtgacaacga aaaaaggagc cgaaagcgtg tcacgctctc aactcctttt
     1081 acgctttatt ttgcttcacg caaaataatc gcttatcgta aactcacgtc attaaacatt
     1141 gacgtaaatt tactctcact catctgactt ttcttattcg gtatcacttc tctgtcacgt
     1201 ccaaacccaa aagccacgtg tctaattctt gtttcaaatt gtctgccgat tctgcaaacg
     1261 tttctcgcaa atgattcgtc aattcatcat tttgactttc ttctttcagc aattcccgat
     1321 atttatattt tgaattcctc gacaaaagag accagaaatt ttgatcagac attaaaccag
     1381 cgagcattat tctatctgta ggtgttgtat caatcggcaa aaaatgcagg ctcgcaaaac
     1441 tattcaaact ttcttgaacc atattatgcc aatcggttgc cttgccatgc cgtagttgca
     1501 tttctaaccg ccaccaagtc tcaacttcct ttggtacaac ttttcgtttg gtttcttgtt
     1561 ccaatttttt attgtatagc cgaatttgtc gttcgctggc acggctaccc caataagctg
     1621 attctagttt tccagaacgc ccgtaaatcg gtttgaacga taccgggtca accacacgat
     1681 attgcataat aaactcatcc ggaacattaa ccatatcgca agcgatatct gcccgcgaaa
     1741 aatggggttc taaaaacaaa tcatgaatga aatttttcat cgaactggct aaaaactgac
     1801 caattttatt gggattaaaa tcaattcgtg ctttcccttc atgaaattgc aagagctcaa
     1861 tatacgcgat attttcttcg tattggtcga caatttgcca gccattttta ccaactgatt
     1921 tgacaaac
//
