LOCUS       NZ_CP061825             2574 bp    DNA     circular CON 07-NOV-2023
DEFINITION  Enterococcus faecium strain A6521 plasmid pA6521_8, complete
            sequence.
ACCESSION   NZ_CP061825
VERSION     NZ_CP061825.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN14675386
            Assembly: GCF_012933195.2
KEYWORDS    RefSeq.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2574)
  AUTHORS   Veeraraghavan,B., Bakthavatchalam,Y.D., Vasudevan,K. and
            Solaimalai,D.
  TITLE     Complete genome of vancomycin resistant Enterococcus faecium_A6521
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2574)
  AUTHORS   Veeraraghavan,B., Bakthavatchalam,Y.D., Vasudevan,K. and
            Solaimalai,D.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-SEP-2020) Clinical Microbiology, Christian Medical
            College, Ida Scudder Road, Vellore, Tamil Nadu 632004, India
REFERENCE   3  (bases 1 to 2574)
  AUTHORS   Veeraraghavan,B., Solaimalai,D., Vasudevan,K. and Arumugam,A.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-APR-2020) Department of Clinical Microbiology,
            Christian Medical College and Hospital, Ida Scudder Road, Vellore,
            Tamil Nadu 632004, India
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 2020
            Assembly Method :: CANU v. v. 1.7; UNICYCLER HYBRID v. v. 0.4.6
            Assembly Name                     :: A6521
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 62.0x
            Sequencing Technology :: Illumina HiSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 11/07/2023 09:17:18
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,203
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,007
            CDSs (with protein)               :: 3,007
            Genes (RNA)                       :: 92
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 40 of 104
            Pseudo Genes (incomplete)         :: 63 of 104
            Pseudo Genes (internal stop)      :: 28 of 104
            Pseudo Genes (multiple problems)  :: 21 of 104
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP061825.1.
            Bacteria and source DNA available from Christian Medical College.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2574
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /strain="A6521"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /plasmid="pA6521_8"
                     /geo_loc_name="India:Vellore"
                     /lat_lon="12.9165 N 79.1325 E"
                     /collection_date="2019"
                     /collected_by="CMC Vellore"
     gene            49..279
                     /locus_tag="HI837_RS16150"
     CDS             49..279
                     /locus_tag="HI837_RS16150"
                     /inference="COORDINATES: protein motif:HMM:NF039481.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6530 family protein"
                     /protein_id="WP_224797045.1"
                     /translation="MPLHRVIDLATLICITMDYSKNGRLSSSDDFPVTRSTDNPELAHH
                     TEFMKKELKQNKSNLDVSLKRLSTELKRLGY"
                     /name="DUF6530 family protein"
     gene            complement(373..777)
                     /locus_tag="HI837_RS15865"
                     /old_locus_tag="HI837_015865"
     CDS             complement(373..777)
                     /locus_tag="HI837_RS15865"
                     /old_locus_tag="HI837_015865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016529521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2251 domain-containing protein"
                     /protein_id="WP_008321396.1"
                     /translation="MTILVTIQAQLVVGEEQVIKSLAPEGMLAAVFEDDGRTGYFYALD
                     ESVEGNPIMDAVHIYNAEDVSDGHIPSDVKIGWSEDSQKCVLLINGYPHGAFDFVGKNG
                     YCRSGYPPPINKVWSLSGHEWSDSVDDFFR"
                     /name="DUF2251 domain-containing protein"
     gene            complement(1571..1972)
                     /locus_tag="HI837_RS15870"
                     /old_locus_tag="HI837_015870"
     CDS             complement(1571..1972)
                     /locus_tag="HI837_RS15870"
                     /old_locus_tag="HI837_015870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009310048.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_012579088.1"
                     /translation="MARVSISEAARLTGKSRTTLHRLIKAGDLSTCSGERNAKMVDTSE
                     LLRVFGPFEQPKGEHHSGQVSGQRDTASADQSEQVIRHLKQEIEHLNTLLLSKDSHIDS
                     LKQAMLLLENHQAKLTESPAPWWKFWKKS"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(1972..2163)
                     /locus_tag="HI837_RS15875"
                     /old_locus_tag="HI837_015875"
     CDS             complement(1972..2163)
                     /locus_tag="HI837_RS15875"
                     /old_locus_tag="HI837_015875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_004187666.1"
                     /translation="MTKQQKTALNMAKFIQNQSLLLLEKLNELDLDVEADLCEKLHDDA
                     EHLFRTLSSRLDALQDGN"
                     /name="Rop family plasmid primer RNA-binding protein"
ORIGIN
        1 tgcattgtcg tcggcagcgt cagatgtgta taagagacaa cagaagagct gcctcttcac
       61 cgggtaatcg accttgcaac tctgatctgt atcaccatgg actacagtaa aaatgggcga
      121 ctttcatcca gtgatgattt tcccgtgact cgctcaactg ataaccctga gctagcacat
      181 cacactgagt ttatgaaaaa agagttaaaa caaaataaaa gcaatcttga tgtaagcctc
      241 aaacgcctct cgactgaatt gaagagactt ggctattaaa actagcttta gaaatgtcta
      301 cctacttcac agacgaattt accataacat ccgtttttcg aaccataatg gcaggctata
      361 gcctgccgta attcaccgga aaaaatcatc cacagagtcg ctccactcat gcccggaaag
      421 agaccacacc ttgtttatag ggggaggata accactcctg cagtacccgt tttttcccac
      481 gaaatcgaat gccccgtgcg ggtatccatt gatcaggagt acgcacttct gactgtcttc
      541 tgaccagcca attttaacat cagaaggaat gtgaccgtct gaaacgtctt cggcattgta
      601 gatgtgtact gcgtccataa tcgggtttcc ctcgacggac tcatcgagcg catagaaata
      661 cccggtccgg ccatcgtctt cgaacacggc ggccagcatg ccctcaggag cgaggctctt
      721 tataacctgt tcctccccga caacgagctg ggcctgtatc gtcactaaaa tggtcatcag
      781 gtctgttctc ctttccttgg ttgcttcggc agccagcttt cagacaagcg gataatttac
      841 cataacatcc gttatcagca tcgtcagaaa cgcgctgagg gcgtttctga cgggaaaccg
      901 gtaaaacagg caacaaacca cccgaaaacc cgccagaaac gcgcaggctc tgtttttacg
      961 gcatgcatga gtatgcacgc aagtgcatag cgcgaaaatt cacgccattc agccagggta
     1021 agtcctgttt ccggcgttct tcccatccga aaacggtgaa cggggacgca atttattgcg
     1081 tggccgtgaa gcgttgccgg cgcagccgga aaaccggttt tcagcacctt caggaatcgg
     1141 gttcgtcgac ggccggcaat cacgttatgg caagtcgccg gacgactcac cacacgattc
     1201 accggcgatt cgaatcgtga attgaatcaa gagtcaccat gagcaaaaaa cacccaaagt
     1261 gatgaattga atcaagagtc gattcatcat aacaatatga taataaagcg tgattcacat
     1321 atgaatcgtg ggcaattcgt cagcgattcg ccatagagtc gtccacgact tggatctgca
     1381 ctttgtaaca catcaccgca catttatttt tcattaaccc attgttgtaa ataaggaatc
     1441 tgcaaaattt taattttgcg tggggtgtgg gtatttttag tggtgaggag cgttcagaga
     1501 tctgacgatt tttgggaagg ttcaggaact ccccgggatg gccggagagg aaaactgaag
     1561 catgacgggg tcaggatttt ttccagaatt tccaccatgg cgccggggat tcagttaatt
     1621 ttgcctggtg attttcaaga aggagcatgg cctgtttcag gctgtcaatg tgcgaatcct
     1681 tggacaaaag taaggtgttc aggtgttcaa tctcctgttt cagatgacgg atcacctgtt
     1741 cactctggtc agcagaggct gtatcacgct gtccagagac ctgtccagaa tggtgttcac
     1801 ccttcggttg ctcaaagggg ccaaagacac gtaaaagctc tgaagtatct accatttttg
     1861 cattgcgctc accgctacag gtggacaggt cacctgcttt aattaacctg tgcagtgttg
     1921 ttctactctt acccgtcagt cgggcagcct cactaataga cactctggcc atcagttacc
     1981 atcctgtaat gcatccagtc tcgaggacag tgtacggaaa agatgctccg catcatcgtg
     2041 gagtttctca caaaggtcgg cctcgacatc gagatcaagc tcgttaagct tctccagcag
     2101 gagcagagac tgattctgga tgaatttagc catgttcagc gctgttttct gttgtttcgt
     2161 cataagaggc ctcagagcgt cagtggtcag tgtagggatc tcctcttttc cgcttcctcg
     2221 ctcactcagt cgctacgctc ctgccgttcg gctgcggcga gcggcaggag catgcgtaga
     2281 aacgctcgag aaaaccgagt aatcgcatgg aggaaagaag gaaaggccgc acagcggccg
     2341 tttttccata ggctccgccc ccctgacgag catcacggaa tctgacgctc aaatcagtgg
     2401 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctgatgg ctccctcgtg
     2461 cgctctcctg ttcctgcctt tcggtttacc ggtgtcattc cgctgttatg gccgcgtttg
     2521 tctcattcca cgcctgacac tcagttccgg gtaggcagtt cgctccaagc tgga
//
