LOCUS       NZ_CP061824             3846 bp    DNA     circular CON 07-NOV-2023
DEFINITION  Enterococcus faecium strain A6521 plasmid pA6521_7, complete
            sequence.
ACCESSION   NZ_CP061824
VERSION     NZ_CP061824.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN14675386
            Assembly: GCF_012933195.2
KEYWORDS    RefSeq.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3846)
  AUTHORS   Veeraraghavan,B., Bakthavatchalam,Y.D., Vasudevan,K. and
            Solaimalai,D.
  TITLE     Complete genome of vancomycin resistant Enterococcus faecium_A6521
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3846)
  AUTHORS   Veeraraghavan,B., Bakthavatchalam,Y.D., Vasudevan,K. and
            Solaimalai,D.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-SEP-2020) Clinical Microbiology, Christian Medical
            College, Ida Scudder Road, Vellore, Tamil Nadu 632004, India
REFERENCE   3  (bases 1 to 3846)
  AUTHORS   Veeraraghavan,B., Solaimalai,D., Vasudevan,K. and Arumugam,A.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-APR-2020) Department of Clinical Microbiology,
            Christian Medical College and Hospital, Ida Scudder Road, Vellore,
            Tamil Nadu 632004, India
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 2020
            Assembly Method :: CANU v. v. 1.7; UNICYCLER HYBRID v. v. 0.4.6
            Assembly Name                     :: A6521
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 62.0x
            Sequencing Technology :: Illumina HiSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 11/07/2023 09:17:18
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,203
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,007
            CDSs (with protein)               :: 3,007
            Genes (RNA)                       :: 92
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 40 of 104
            Pseudo Genes (incomplete)         :: 63 of 104
            Pseudo Genes (internal stop)      :: 28 of 104
            Pseudo Genes (multiple problems)  :: 21 of 104
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP061824.1.
            Bacteria and source DNA available from Christian Medical College.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3846
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /strain="A6521"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /plasmid="pA6521_7"
                     /geo_loc_name="India:Vellore"
                     /lat_lon="12.9165 N 79.1325 E"
                     /collection_date="2019"
                     /collected_by="CMC Vellore"
     gene            complement(join(3092..3846,1..226))
                     /locus_tag="HI837_RS15845"
                     /old_locus_tag="HI837_015845"
     CDS             complement(join(3092..3846,1..226))
                     /locus_tag="HI837_RS15845"
                     /old_locus_tag="HI837_015845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355134.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006260 - DNA replication [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="WP_014387154.1"
                     /translation="MKKKKQDLINGTFREKKRNLKKIMAFGDGRISGKTLENMKACGSF
                     MEFIATVDKEKKKMVHGMFCKNRFCPVCAYRKARKDSYMLSIMMEAIQAEKGYEYIFMT
                     LTTPNVPADKLSEEIDRFNKALSKLFRRKRVTTSIKGYVRKLEITYNKKRNDYNPHFHL
                     ILAVNKSYFTDKDYYIKQSEWLDLWRDVTGLDGINEDGTDEICSLDVRKVKGFQQDKAV
                     SEIAKYSAKDFEITYSQDVFDTFYFAMKGRQLMTFNGVFKEYRKKYESGELDHYKKKDE
                     NDYFWFLTASWMQKDANYSVAVRELTDDEKKFFAERYDKEGDETG"
                     /name="protein rep"
     gene            complement(814..2220)
                     /locus_tag="HI837_RS15850"
                     /old_locus_tag="HI837_015850"
     CDS             complement(814..2220)
                     /locus_tag="HI837_RS15850"
                     /old_locus_tag="HI837_015850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173818.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid recombination protein"
                     /protein_id="WP_138222786.1"
                     /translation="MGFSISFKKGTKNTSISHNNRELTDSQKINDWHKHIDFTKSDENI
                     YLEQINIREKYEELFGEAVEKYNAKQKRADRKIEDYLAKVRKDKKMEPQREFIVQIGTL
                     DDFRTTRDDGSSTGISEQQAEQNRVIANKILVEYFKEFKERNPSLSVYNAVIHNDEVSP
                     HLHLNIVPVAEGYKRGVQKQPSFNKALQQQGLRADKENGRVIWNNFRNQEVESIERLMA
                     DFGWERELVGTNKIKDIHEYKEIMSEISELRQTAVKERESVSDELRYIYRQREENEREK
                     ALVASERAEIEEEKKNMLNVKKEMAVIFQKDMVPLESFKRLPDGSYHLSGNDFVDLYQR
                     ATRATNAKRNADNMSEQYLQMLEQNTELNAYVAALEEERHLSGRKQLADLQVENKKLKE
                     ENRSLRQRLERLKNAFQKSITRLSIRFGLAEKDMGLSEELEVLEQEKTLFYRQNVSQHK
                     LERSEDEIEW"
                     /name="plasmid recombination protein"
     gene            complement(2442..2804)
                     /locus_tag="HI837_RS15855"
                     /old_locus_tag="HI837_015855"
     CDS             complement(2442..2804)
                     /locus_tag="HI837_RS15855"
                     /old_locus_tag="HI837_015855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002318183.1"
                     /translation="MGVTLADIKAQEEKLKKEQAKLRQQKKRLIEKENAEMGKALRKHF
                     GFESFADFERWLSSISDDQQHEKSVDNEWLADEKSAPSERDYPDESDGLFGVIPNKKSQ
                     MSESKFTSIFNDVNLR"
                     /name="hypothetical protein"
     gene            complement(2857..3105)
                     /locus_tag="HI837_RS15860"
                     /old_locus_tag="HI837_015860"
     CDS             complement(2857..3105)
                     /locus_tag="HI837_RS15860"
                     /old_locus_tag="HI837_015860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002323248.1"
                     /translation="MKLDKQRLYEAVKDNCNCGGNRQLFKAMMVTVYRRLDGENAETAV
                     ENAYFQVFGEHHSPEWDRAILDDMAEMHEKLWRALND"
                     /name="hypothetical protein"
     Typing          complement(3093..3846)
                     /name="rep_cluster_1077"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(818..2220)
                     /name="MOBV"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.573"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 ggtatgcaca aaccggacaa aatcggtttt tacagaacat gccatgaacc atctttttct
       61 tctccttgtc aaccgttgct ataaactcca tgaatgatcc acaggctttc atgttttcca
      121 aggttttgcc agaaatacgg ccatcaccga acgccatgat tttctttaaa tttctctttt
      181 tttctcgaaa tgttccgtta atcaaatctt gctttttctt tttcatcttg ctatactgaa
      241 tctacaaatt ttgtatacaa aaaaggctga aaagccgata acaaaaaata gattgctctc
      301 ctttctagtt tagttttttt ctagtcaatt aaaactataa aatactaggg aagaaaaagc
      361 aatctttttt tttgttcttc cttagagcca caagggaaaa gaccatacat aggcacgatt
      421 ttctcccgaa aatagttacc ctatgtatca agataagaaa aaacatcaaa aaagggggcg
      481 aaaatcgccc cctttttttc gttttaaaac cccaaaaccc aaagacataa aattcggtca
      541 attgaccact gcatttttag gggaagggtc aaactttgat actttaaccg cctgttagag
      601 agggctgtta atttttccca ctcattctta gtcatgcaaa cataaaaccg caccctttga
      661 aaagggcgcc cccaaacttc gctaacgcta ggttggccaa aaaatggact ggccattttt
      721 tagcaaatgt cagcttaata ggctagtggt ttagccgtca actccttatc ctcggcacaa
      781 accgcctccg gtaccgttga ctgcacatcg taactaccac tcgatctcat cttcagaacg
      841 ctctaattta tgctgtgaga cgttttgacg ataaaataag gttttctcct gctctaatac
      901 ttctaattcc tcagaaagcc ccatatcctt ttctgcaaga ccaaaacgga tagacaaacg
      961 agtgatcgat ttttgaaatg cgttttttaa tcgctctaaa cgctggcgta aagagcgatt
     1021 ttcttctttc agctttttat tttccacttg taaatctgct aattgttttc taccagacaa
     1081 atgacgctct tcttccaatg cagctacata cgcatttaat tctgtatttt gctcaagcat
     1141 ctgtaaatat tgctcactca tgttatcagc atttctcttg gcatttgttg ctctcgtagc
     1201 tcgttgatac aaatcaacaa aatcattccc agataaatga tacgaaccat caggaagcct
     1261 cttgaaactt tctaaaggaa ccatatcttt ttggaaaatg accgccatct ctttttttac
     1321 attcagcata tttttcttct cttcttcaat ctccgctctc tcagaagcca ccagagcctt
     1381 ctcacgctcg ttttcttctc tttgcctata aatatacctc aactcatcag aaacgctctc
     1441 acgctctttt acggccgttt gacgcaattc ggaaatttcg ctcatgattt ctttgtattc
     1501 atgaatatct ttgattttgt tagtaccgac taactcacgc tcccaaccaa aatctgccat
     1561 caaacgttca attgactcaa cttcctgatt cctaaaatta ttccaaatca ctcgaccatt
     1621 ctctttatct gctcgtaaac cctgctgttg caaggctttg ttaaaacttg gttgcttttg
     1681 aacaccccgt ttataccctt cagcaacagg aacaatattc aaatgcaggt gcggacttac
     1741 ttcgtcatta tgaataacag cattgtagac ggataaacta ggattccgtt ctttaaattc
     1801 tttgaaatac tcaactaaaa ttttgttggc aatcactcga ttttgttctg cttgttgttc
     1861 cgatatgcca gtagaagaac catcatcacg agtagtacga aaatcatcaa gagtaccaat
     1921 ttgcacgata aattctcgct gtggttccat ttttttatcc ttgcgaacct ttgctaaata
     1981 gtcctcgatt tttctatcgg ctcttttctg ctttgcatta tatttttcta cggcttcacc
     2041 gaataattct tcatattttt cacgaatatt tatttgttcc aaatagatat tttcatccga
     2101 ttttgtgaaa tcaatatgct tatgccagtc attgattttt tggctatcag ttaactcacg
     2161 attattatga gaaatgcttg tattctttgt tccctttttg aacgaaatac taaaccccaa
     2221 gttagacccc tccttcgcgc tgtcgctaat aaaaagagtg taaacctttc tgttactctt
     2281 tgacaaagag taacattttc ctaattgtga catggctgtc acaattccaa atgctctccg
     2341 agggtattcg ctgtcacaag tgacaacgaa aaaaggagcc gaaagcgtgt cacgctctca
     2401 actcctttta cgctttattt tgcttcacgc aaaataatcg cttatcgtaa attcacgtca
     2461 ttaaatattg acgtaaattt actctcactc atctgacttt tcttattcgg tatcactccg
     2521 aataagccgt cagattcatc gggataatct cgttcgcttg gcgctgattt ttcatcagca
     2581 agccactcat tatccactga tttttcgtgt tgctgatcgt cagaaatcga cgatagccac
     2641 cgctcaaaat cagcaaaaga ttcaaaccca aaatgcttgc gaagcgcctt gcccatttca
     2701 gcattttcct tctcaatcaa tcgctttttc tgttggcgaa gttttgcctg ctcttttttc
     2761 aatttttctt cttgtgcttt aatatcagct aaagttactc ccatgaatta caactcctta
     2821 cccttagatt catccctata aataacacca atttgattag tcatttagcg ccctccacaa
     2881 tttctcgtgc atttccgcca tatcatcaag aatagcacga tcccattcag ggctatggtg
     2941 ttcaccgaac acttgaaaat aagcattttc cacagctgtc tcagcatttt ccccatccaa
     3001 ccgacgataa acagtcacca tcatagcctt aaaaagctga cgatttccgc cacaattaca
     3061 attatctttc accgcttcat atagcctttg cttatccagt ttcatcaccc tccttatcat
     3121 agcgttccgc aaagaatttc ttctcatcat ccgtcaactc acgaacggcc accgagtaat
     3181 tggcatcctt ttgcatccag ctagcagtca agaaccagaa atagtcgttt tcatcttttt
     3241 tcttgtagtg atcgagttcg ccactctcgt attttttcct gtactccttg aacacgccat
     3301 tgaaagtcat cagctgacga cctttcatag caaaatagaa cgtgtcaaaa acatcttggg
     3361 aataggttat ttcaaaatct ttggcagaat atttggcaat ttccgatacc gctttgtctt
     3421 gttggaatcc tttgaccttg cggacatcca acgaacaaat ttcatcggtg ccatcctcgt
     3481 taatgccatc caaaccggtg acatcccgcc acaaatctaa ccattccgat tgcttgatat
     3541 aataatcttt atccgtgaaa taagatttat ttactgccaa aatcaagtga aaatgcgggt
     3601 tatagtcatt ccgttttttg ttgtacgtga tttccaattt tcgcacatag cccttgattg
     3661 aagttgtcac cctttttcgc cgaaacagct tcgacagcgc tttgttgaaa cgatcaattt
     3721 cttcggataa tttatccgca ggcacattgg gtgtggtgag ggtcataaag atatattcat
     3781 accccttttc agcttggata gcttccatca tgatagacag catatagctg tccttacggg
     3841 cttttc
//
