LOCUS       NZ_CP061822             4465 bp    DNA     circular CON 07-NOV-2023
DEFINITION  Enterococcus faecium strain A6521 plasmid pA6521_5, complete
            sequence.
ACCESSION   NZ_CP061822
VERSION     NZ_CP061822.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN14675386
            Assembly: GCF_012933195.2
KEYWORDS    RefSeq.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4465)
  AUTHORS   Veeraraghavan,B., Bakthavatchalam,Y.D., Vasudevan,K. and
            Solaimalai,D.
  TITLE     Complete genome of vancomycin resistant Enterococcus faecium_A6521
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4465)
  AUTHORS   Veeraraghavan,B., Bakthavatchalam,Y.D., Vasudevan,K. and
            Solaimalai,D.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-SEP-2020) Clinical Microbiology, Christian Medical
            College, Ida Scudder Road, Vellore, Tamil Nadu 632004, India
REFERENCE   3  (bases 1 to 4465)
  AUTHORS   Veeraraghavan,B., Solaimalai,D., Vasudevan,K. and Arumugam,A.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-APR-2020) Department of Clinical Microbiology,
            Christian Medical College and Hospital, Ida Scudder Road, Vellore,
            Tamil Nadu 632004, India
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 2020
            Assembly Method :: CANU v. v. 1.7; UNICYCLER HYBRID v. v. 0.4.6
            Assembly Name                     :: A6521
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 62.0x
            Sequencing Technology :: Illumina HiSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 11/07/2023 09:17:18
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,203
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,007
            CDSs (with protein)               :: 3,007
            Genes (RNA)                       :: 92
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 40 of 104
            Pseudo Genes (incomplete)         :: 63 of 104
            Pseudo Genes (internal stop)      :: 28 of 104
            Pseudo Genes (multiple problems)  :: 21 of 104
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP061822.1.
            Bacteria and source DNA available from Christian Medical College.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4465
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /strain="A6521"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /plasmid="pA6521_5"
                     /geo_loc_name="India:Vellore"
                     /lat_lon="12.9165 N 79.1325 E"
                     /collection_date="2019"
                     /collected_by="CMC Vellore"
     gene            581..1504
                     /locus_tag="HI837_RS15810"
                     /old_locus_tag="HI837_015810"
     CDS             581..1504
                     /locus_tag="HI837_RS15810"
                     /old_locus_tag="HI837_015810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296860.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_002351085.1"
                     /translation="MANEIVKYENRLNHIPLRKFNSREMNLFFSIASRVREKGVDEIIF
                     SFHDLKKLSGYTERGEQFIKDLSSTYDKLLSINAWNDDGNTLTKFVAFTKYSIIRDKQL
                     VIISVNPEFKGLFNQLKNWTRFNLEQFTNLDSTYSKTMFRLLKQYRTQGWTEFSKEKFF
                     DLLDIPKSYWNSPSNIDKFILRPIKEELTPLFKGLTIRKKYGKGRGKPVIGYRFTWKAE
                     INHADDFSKGKQEDLRIKLFNIDHNGELTREEKWRAKDRILNLPLGTHEADFKQQQQTE
                     EKQAEKRELSNKLKQDLLGNLQNLFD"
                     /name="replication initiation protein"
     gene            1520..2059
                     /locus_tag="HI837_RS15815"
                     /old_locus_tag="HI837_015815"
     CDS             1520..2059
                     /locus_tag="HI837_RS15815"
                     /old_locus_tag="HI837_015815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016616811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="WP_002351084.1"
                     /translation="MADKTIKELAEELGVSKTAINKKVSDKNRKLWFAKNGNKFVINET
                     GQKAIKSMFLVDNANLNRKLVGEKSKTKNHKNTNQVSELKLLSFLEKQLNRKDIQLQEK
                     DNQIKQMQKLLDQQQQLTLQANQQIENLQEQLHLTYTEETPTTESTAFSEKKADNTQPL
                     IEKKWWQIWKSNRTNL"
                     /name="DUF536 domain-containing protein"
     gene            2744..2917
                     /locus_tag="HI837_RS15820"
                     /old_locus_tag="HI837_015820"
     CDS             2744..2917
                     /locus_tag="HI837_RS15820"
                     /old_locus_tag="HI837_015820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_010729871.1"
                     /translation="MLKKTVLVLGSISLGILGFSPMLLSVIGKNNSNGELKLNSNYYNS
                     PNEELFVKETDK"
                     /name="hypothetical protein"
     gene            3322..3756
                     /gene="bacA"
                     /locus_tag="HI837_RS15825"
                     /old_locus_tag="HI837_015825"
     CDS             3322..3756
                     /gene="bacA"
                     /locus_tag="HI837_RS15825"
                     /old_locus_tag="HI837_015825"
                     /inference="COORDINATES: protein motif:HMM:NF041453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin 51 precursor BacA"
                     /protein_id="WP_002351079.1"
                     /translation="MKNKIIKIITCLFLFGMIVGASTPLNLFPFSARGIKVEAASSRYN
                     HNHRGFTCYKMNYYVTNKMCRDLKKNYNKLKTPAQIASFIPIGGVGTWVITNAFSSAVD
                     AMNVFVRAANQGKGVQLTYNAHFSNTTSYQYNDYARYVIK"
                     /name="bacA"
     Typing          582..1504
                     /name="rep_cluster_1291"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 tttaagagtg cttaaatcga attttagagg gcatttagac aattttaggg ttgttagggc
       61 ttgccctgac cgcctgtaag gcgttttaag aacaagataa gggatttata ctaggactgt
      121 cttaaaatgt cttaaacgaa cgctcagagc ttctcaaagg gttccttatg aacggctttt
      181 agcaaaggag ccggaggcga ctgcgcagta taatgtgaga gcagagcgat cacacctttt
      241 tagcgaaccg tagggagcaa ggtttggaat tggcagtttt gataaattcg cgaacctgtt
      301 tagagaaaaa aggagtgatt ttttaggcgg gaaaattagc ctcccctctt tttatatctc
      361 tttttatatc tctttttata tctcttttat gtcggctgtg tccttactct cccaagcaat
      421 tcagagtttt ttcaagtagg ttttctacgc ttttcaagta ggttttctac gcttttcaag
      481 taggttttct acgcttttca agtaggtttt ccgacattat tgagtatttt tataaagtat
      541 gttactttta tttcatactt tataaaagta ggagaagctt atggcaaatg aaattgtaaa
      601 atatgaaaat agattaaatc atattcctct gagaaaattt aatagtcgtg aaatgaactt
      661 atttttttca attgcttcac gtgtacgtga aaaaggtgta gatgagatta ttttttcatt
      721 tcacgatttg aaaaaactaa gcggttatac agaacgtggg gaacaattta taaaagatct
      781 tagtagcacg tatgacaaat tattaagtat taatgcttgg aatgatgatg gaaatacttt
      841 aactaaattt gttgctttca caaaatatag cattatacga gataaacaac ttgttataat
      901 ttcagttaat ccagaattta aaggactttt taatcaatta aagaactgga caagatttaa
      961 tctagagcaa tttactaatt tggatagcac atattcaaaa actatgtttc gattattaaa
     1021 acaatatcga actcaaggct ggactgaatt ttccaaagaa aaattttttg atttactaga
     1081 tattcctaaa agttattgga atagtccgtc aaatattgac aaatttatct taaggccaat
     1141 taaagaagaa ctcactccct tatttaaagg attaactatt cgtaaaaaat atggtaaagg
     1201 tcgaggtaaa cctgttatcg gttatcgttt tacttggaaa gctgaaatta accatgctga
     1261 cgatttttcc aaagggaaac aagaagattt acgtataaaa ttatttaata ttgatcacaa
     1321 tggagaatta acacgagaag aaaaatggcg cgctaaagat cgaatactaa atcttccctt
     1381 aggcactcat gaagctgatt ttaaacaaca acaacaaact gaagaaaaac aggcagaaaa
     1441 acgagaactt tcaaacaaac tcaaacaaga tttactagga aatttacaaa acttattcga
     1501 ttaagcttaa ggagtattta tggcagataa aacaatcaaa gaacttgcgg aagaattagg
     1561 agttagtaaa acagcaatta acaaaaaagt ttctgacaaa aatcgaaaac tttggtttgc
     1621 aaaaaacgga aacaagtttg tgattaacga aaccggtcaa aaagctataa aatcaatgtt
     1681 tttagtcgat aacgcaaact taaatcgaaa actagttggc gaaaaatcaa aaaccaaaaa
     1741 ccataaaaac accaaccaag tttccgaatt aaaactcctt tctttcctag aaaaacagtt
     1801 aaatcgaaaa gatatacagc tacaagaaaa agataatcaa ataaaacaaa tgcaaaaatt
     1861 actcgaccag caacaacaac tcactctaca agcgaaccaa caaattgaga acctacaaga
     1921 acagctccat ttaacttata cagaagaaac tccaacaacc gaatcaactg ccttttctga
     1981 aaaaaaagca gacaatacac aaccacttat tgaaaaaaag tggtggcaaa tttggaaatc
     2041 taatagaacc aatctctaaa attttctaga agttagttcg gatagtagag gaaaaagttg
     2101 tactacctga tggggagatc actcaaattt ttatgagaaa gaaaaaatag tttacaactg
     2161 taaacaatca atgtttatag ttgtaaacta tcaaactata tggtgactta ttgcttaaaa
     2221 taggttgaaa tctcactcaa tttttggtgg aaaaacaaaa taaattctaa tcgattttgt
     2281 tgaaaaagtc ataaaaaaac aaatgtgatt ttaacaaaat ttcaaaaaaa tcgtttgaaa
     2341 tttcgtttat tttttttgag aattttgatc atcgtagggt cattgtagaa tcattatgag
     2401 atcatcgtgg gatcatcgaa taaagtatgg gaaaggcttg cggtgcttgg tattaagggc
     2461 attctccgcc tcccaggttc ttgaccattt tctctcaacg tggtaaaaat cccacttata
     2521 agagaaaatg gtcaagaacc taaacggtcg gcgaagaaaa cataccaagc accacaagca
     2581 aaaaaagtta gagtaacttc caaaaaagaa aaatttttta gaacaacttt tgaaaattta
     2641 attttgattt atatccgttc aatttaaaca tttgataaac tgaaggctag tatttaaaat
     2701 gtgtatacaa gtggtagcgt taaaaaaagt ggaggtataa cctatgttaa aaaaaactgt
     2761 gttggtactg ggttctattt ctctaggaat attaggtttt agccccatgc tattatccgt
     2821 aattggaaaa aacaattcca acggagaact taaattaaat tcaaattatt acaacagtcc
     2881 taacgaagaa ttgtttgtaa aagagacgga taaataataa aaaagactgc tctttcgagc
     2941 agccagttaa aaaataatga gtgtttattc ttgaaaaagc aacgcgctta tgaagcgcta
     3001 ccaaaaattt atcatatttt atgaaaaata acaccccagt aaaaaatgac tggtaaaaat
     3061 tcacaaagaa acggaaagaa gcaactgcag aaaatcaagc cttaaaaaga gcttttgact
     3121 tagaccaatc gaaaatcaca aagtgagccc ctgtatccct tggtagagag gctacttggc
     3181 atagtcgaca ttatgtctac taacctttaa aataacacgt tttataaaat aaaccctctt
     3241 gacaatattt aattttctga ttattctaaa tatatacaga aagcgctttc ctaggatttt
     3301 atatctaagg aggaagtaac aatgaaaaat aaaattatta aaattattac ttgtctattt
     3361 ttgttcggta tgattgttgg agcaagtact ccgttaaatc tgtttccttt tagtgctaga
     3421 ggtataaaag tggaagctgc aagttcacga tataatcata atcatcgtgg atttacttgc
     3481 tataaaatga actattatgt tactaataaa atgtgtagag atttgaaaaa aaattataat
     3541 aagctaaaga ctcctgctca aattgcatct tttattccta ttggtggagt tggaacatgg
     3601 gttattacaa atgcttttag tagtgctgtt gatgcaatga atgtctttgt aagagctgca
     3661 aatcaaggaa aaggtgtaca attaacatat aatgctcact ttagcaatac aacatcatat
     3721 caatacaatg attacgctcg ttatgtaata aaataatcag aaagtgaaac tatctcatga
     3781 ataaaaagat tttagctgta attatttcaa taatagtttt aatcactatg ttcttcatat
     3841 ttaggctaat tttcaatata agcttacaaa aaagtattct ttatctaatt cctatagcat
     3901 ttatcttagc aatttttaga agtatttacg gaaataaaaa ctctaaatag ctaaaaataa
     3961 atcatactaa attttacaaa aagtggctag cattttctag ccactttttg tattgtggag
     4021 cgtgggtttt gagggggatt caagggggag cttgcgcccc tttgacaagc ggttatttgc
     4081 accaaatacg gaggtatttg gctagcaaat atagagcttg ccaaaccctt ttagtgagtt
     4141 ttgtagaaag tacctttaaa ggtactttca cattacgaac gatacgtccg taggacgtac
     4201 tgaaagggtg ggacttatga gcaaattaat gcaaaatttg gctagcaaaa aagctaaaaa
     4261 atatatgcta ataatcgagc gaaataaaca ctttttcaag tgagcgaatc cgccatgaca
     4321 aagctgaagc agtttatttc agctttgtca tggcgtgtgc cgactttttt aaaagtctag
     4381 gcacacgagc aaatatcttt atcaagctct aatttgcgtt ttaagacgtt tgaaacgcct
     4441 aagtatataa atatataaga atagg
//
