LOCUS       NZ_CP061752             5472 bp    DNA     circular CON 15-JUN-2023
DEFINITION  Escherichia coli O15:H12 strain 2273-PO3 plasmid unnamed3, complete
            sequence.
ACCESSION   NZ_CP061752
VERSION     NZ_CP061752.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN16183875
            Assembly: GCF_014770655.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli O15:H12
  ORGANISM  Escherichia coli O15:H12
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5472)
  AUTHORS   Guragain,M., Brichta-Harhay,D.M., Bono,J.L. and Bosilevac,J.M.
  TITLE     Locus of Heat Resistance (LHR) in Meat-Borne Escherichia coli:
            Screening and Genetic Characterization
  JOURNAL   Appl Environ Microbiol 87 (7), e02343-20 (2021)
   PUBMED   33483306
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5472)
  AUTHORS   Guragain,M. and Bosilevac,J.M.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-SEP-2020) US Department of Agriculture, US Meat Animal
            Research Center, State Spur 18D, Clay Center, NE 68933, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Microbial assembly v. Oct-19
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 359x
            Sequencing Technology             :: PacBio Sequel
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 06/15/2023 02:52:05
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,193
            CDSs (total)                      :: 5,078
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 115
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 294
            CDSs (without protein)            :: 294
            Pseudo Genes (ambiguous residues) :: 0 of 294
            Pseudo Genes (frameshifted)       :: 71 of 294
            Pseudo Genes (incomplete)         :: 222 of 294
            Pseudo Genes (internal stop)      :: 45 of 294
            Pseudo Genes (multiple problems)  :: 42 of 294
            Pseudo Genes (short protein)      :: 3 of 294
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP061752.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5472
                     /organism="Escherichia coli O15:H12"
                     /mol_type="genomic DNA"
                     /strain="2273-PO3"
                     /serotype="O15:H12"
                     /isolation_source="finished sheep/lamb carcass in sales
                     cooler"
                     /host="Ovis aries"
                     /db_xref="taxon:2773704"
                     /plasmid="unnamed3"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2005-05"
                     /collected_by="Norasak Kalchayanand-USDA/ARS"
     gene            complement(1..1020)
                     /locus_tag="IED00_RS25540"
                     /old_locus_tag="IED00_25540"
     CDS             complement(1..1020)
                     /locus_tag="IED00_RS25540"
                     /old_locus_tag="IED00_25540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_001271364.1"
                     /translation="MSAALQYFEENLPHRPYHTDDLAFGLRISGKGRALLARYIQQNQP
                     HAQFWLVFDVDREGAAIDWSDRNAPAPNITVKNPVNGHAHLLYALNIAVRTAPDASVKA
                     LKYAAAVERSLCEKLCADVNYSGLICKNPFHLEWQVMEWREDAYTLDELADYLDLSASE
                     RRSIDKHYGMGRNCHLFEMTRKWAYRAIRQGWPAFSQWLEAVIQRVEMYNASLPVPLSL
                     AECRAIGKSIAKYTHRNFTPETFAQYVADTHTPEIQAARGRKGGKANSSENQSDKGKKS
                     AAVRWTANDDKRRRALDMYILGASTEDIAVAVGVSSRTIRRWMDNSGEWLTKKQIIKC"
                     /name="replication initiation protein"
     gene            complement(1829..2953)
                     /locus_tag="IED00_RS25545"
                     /old_locus_tag="IED00_25545"
     CDS             complement(1829..2953)
                     /locus_tag="IED00_RS25545"
                     /old_locus_tag="IED00_25545"
                     /inference="COORDINATES: protein motif:HMM:NF013211.2"
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA];
                     GO:0050829 - defense response to Gram-negative bacterium
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like pore-forming protein"
                     /protein_id="WP_021529456.1"
                     /translation="MSGGDGRGPGNTGLGHNGGKPSGNVNGSSGHGGPSSGGGYHYNPG
                     KGPASTVGPDGEVHINITGGMEKDPPGKHHGNGGSGNGHGAGNHNSNSGGNGKTTSIPG
                     WSPDSGKPGRYLPTNKDKLIKGLIQSIDDHSGTYTIHYTGGAVATVTVPNGDVNKMNVH
                     YNIGSGPSSGNKLTRDTIKSFLEFKAENEANAVNDAISKVNDFYKELASKYGDKLEKEA
                     KALADEAKGKKIRNANEAIKAFDRYKDVLNKKFSVSDREAIAKALESLNKDQMAKQLKI
                     FGKAFGVVGEAIQWGGFISGLVKGFRTGDWNEAFISGEKIAVGKVASVMVTVAFSAMAV
                     NPIGILGFAVIMAVTSALITDERLKQLNSFINGI"
                     /name="colicin-like pore-forming protein"
     gene            complement(3174..3707)
                     /locus_tag="IED00_RS25550"
                     /old_locus_tag="IED00_25550"
     CDS             complement(3174..3707)
                     /locus_tag="IED00_RS25550"
                     /old_locus_tag="IED00_25550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_021539385.1"
                     /translation="MASTEEKLLTLLLSVEHLQHTAMEQQKAVADSLAVARETLRGYRD
                     ISDGVAKQVRDEVRNEVQNLDVAGLIGERISQDFQRLEESVANMKANADALNRDIATIH
                     SNIVQEYNTLKGYSWKWLGGVSVAFLVMFCVLGWGMKTMLDRNYNATAAVYQKVVILED
                     KLPEKPVTGKNRKN"
                     /name="hypothetical protein"
     gene            complement(3710..4636)
                     /locus_tag="IED00_RS25555"
                     /old_locus_tag="IED00_25555"
     CDS             complement(3710..4636)
                     /locus_tag="IED00_RS25555"
                     /old_locus_tag="IED00_25555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="WP_001736644.1"
                     /translation="MATYHLSVKFGGKGQAANHADYIERKEKYRDRQDLEYSAHGNMPE
                     WARDNPSHFWQAADQFERANGSTYRELEIALPRELTPEQRLELVQDFVRQEAGERHAWS
                     FAIHNPKASIEGGEQPHAHIMMSQRVNDGIERTPEQYFRRYNARYPERGGAKKDSGSLT
                     PTQQKEQLRELRKRWEVKHNEHMRKNGFERGFIDCRTLKEQGIERRPEVHLGFEAAGRL
                     DDAQRHDIMQKRSEPDYSRHLEQARTVGHDIMEWLDTPVKQHELVRRLQHDVAREHGFD
                     MRDRVSRQDRTEPERVKSPSRGFSMGF"
                     /name="MobA/MobL family protein"
     gene            4813..5211
                     /locus_tag="IED00_RS25560"
                     /old_locus_tag="IED00_25560"
     CDS             4813..5211
                     /locus_tag="IED00_RS25560"
                     /old_locus_tag="IED00_25560"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001222916.1"
                     /translation="MRISNIEWLKKRIGFIRKLGEQTARQRQIIDLLDNEAGLTEQERK
                     LLHVLATAEKNDLQAQESERKQAVQKRIEGKKQRRERNHRLFLAAGLLIEAGLVDTKTG
                     ELCYKKDRILQALKEIKYDLETSPNPDA"
                     /name="hypothetical protein"
     Typing          complement(246..1020)
                     /name="Col156"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="96.907"
                     /coverage="83.0"
                     /evalue="0.0"
     Typing          complement(3714..4636)
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="98.052"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 ctaacattta attatttgtt tcttggttag ccattctcca gaattatcca tccagcgtcg
       61 aatagtccgg ctgctaacac caaccgccac tgcaatatct tccgttgacg ctcccaagat
      121 atacatatca agcgccctac gacgtttgtc gtcgttagca gtccaccgaa cagcggcgct
      181 ttttttccct ttgtccgact gattttctga cgaattggcc ttcccgccct tgcgaccgcg
      241 tgctgcctga atttctggcg tgtgcgtatc agccacatac tgtgcgaaag tttccggcgt
      301 gaagttcctg tgcgtgtatt tcgcaatgct cttgccaata gcccgacatt cagccagtga
      361 aagcggaacg ggaagcgatg cattgtacat ttcgacacgc tggatcaccg cttcaagcca
      421 ctgtgagaac gctggccagc cctgacgaat cgccctgtaa gcccatttgc gcgtcatttc
      481 gaacaggtgg cagtttcgcc ccatcccgta atgtttatcg atgctacgac gctctgaggc
      541 gctcaaatcg agataatctg ccagttcatc gagggtatag gcgtcctcgc gccactccat
      601 cacctgccat tccaggtgga acggattttt gcaaatcagg ccgctgtagt tcacatcagc
      661 acaaagtttc tcacacagcg aacgttcaac cgctgcggca tatttcagcg ccttaaccga
      721 ggcatcaggc gcggttctca cggcgatgtt gagtgcgtag agcaggtgag cgtgtccgtt
      781 cacaggattt ttaacggtga tgttcggcgc gggggcgttc cggtcgctcc agtcaatcgc
      841 agccccctcg cggtcaacgt caaaaaccag ccagaactgc gcatgaggct ggttctgctg
      901 gatgtaccgc gcaagaagcg cacgcccttt gccggagatg cgaaggccga aagcgagatc
      961 atccgtgtga taggggcggt ggggtaaatt ttcttcgaag tattgaagcg cggcgctcat
     1021 gtgttacgat tccttccagg ttatcgaccg acctcgcccc tgtattctcg tgacaatctt
     1081 ggcggagaga cacgaatttc aggggcgttt gtttttcagt cgcttacact acccctaaca
     1141 acaaaacctg tcgagccttc ccgcaaaacc gatccattta ccataactcc cgttgtacgt
     1201 accccttaaa acgccctcag tcgctttctg gcgcaatcgc tgataaaacg caaaacgaaa
     1261 aggaaacacc aggccaaacc agaaaaannc gtcagaanca ctttcagggc gttgcataac
     1321 tatgcacgaa agcgattcgc aaacagtttc gaccgttcaa aagggcgaac gagtcaaaaa
     1381 agtaactttt gtgataacta cttctagatt tatggataaa gtaaggcaat attataaaca
     1441 tttaactaac gaggatttta taaatgaaaa aaatagatat ggaattctat ttaaggaatt
     1501 ttatccttgc cttatccttt tacattttat cttttatcat cgcaagtttg aatctaagcc
     1561 caacaagtat agaattattt atatttatac ttatatctct ttttggatcg tccacatacc
     1621 ctttttgtct attactaata aataagcaag cacccttact aacaaaaaca acttttaact
     1681 tcgttaactg gggttttata atatgcatta tattatcact acctccattt ggattgattt
     1741 ttacagtcta ttactttata accaacaaaa agcgatagaa aaacaaaata aaggtcatac
     1801 catatcaaac ggtacgacct tttgtttatt aaatgccgtt aataaagctg tttagttgtt
     1861 tcagcctttc atctgtaatt aaggcactag tcactgccat aattactgca aaccctaaaa
     1921 taccaatagg atttactgcc atagcactaa atgccactgt aaccataaca gatgctacct
     1981 tgccgactgc gattttttcc ccgcttataa aagcctcatt ccagtcacca gttctgaatc
     2041 ctttaactaa gccactgata aatccccccc actgaatagc ctcacctacg acaccaaagg
     2101 ccttaccaaa aatttttaac tgtttagcca tttgatcttt attaagagac tccagtgctt
     2161 ttgcaatcgc ttctcggtcg gatacgctga atttcttatt aagcacatcc ttgtacctat
     2221 caaaggcttt aatcgcctca ttagcattgc gaattttttt ccctttagct tcatcggcta
     2281 gtgccttggc ttctttctcc aacttatcgc cgtattttga tgctaactct ttataaaagt
     2341 cattaacttt gcttattgca tcatttacag cattagcttc attttctgcc ttgaattcta
     2401 aaaagctttt tatagtatca cgagtcagtt tattacctga tgacggccca ctaccgatat
     2461 tatagtgaac attcatttta ttcacatcac cgtttggtac agttactgta gctactgcac
     2521 caccagtata atggatagtg tacgttccag aatggtcatc tattgactga ataagtccct
     2581 taattaactt atctttattt gtcggtaaat atctgccagg tttcccagag tcagggctcc
     2641 atccaggaat agacgttgtt tttccattgc cgcctgaatt gctgttatga tttccagcac
     2701 cgtgaccatt acctgagcca ccattaccgt gatgttttcc tggaggatct ttttccatac
     2761 caccggtaat gttgatatgc acctccccgt caggacctac agtgcttgca ggaccttttc
     2821 ccggattgta atgataccca ccgccagatg atggtcctcc atgtccagat gagccattca
     2881 cgtttccact gggtttacca ccgttatgtc caagccctgt attaccaggg ccacgaccat
     2941 ctccaccact cataaaaatt ccctctttta atcgttaaaa caattaatta catagttaat
     3001 actgtacata aaaccactgg ttttatgtac agatcaaaaa gtactcgcca ttattttttc
     3061 tgtcaagtca gggggggata ttttttggca gttcggagga atcactcatg aaaacggtga
     3121 acggaacgcg aaatcctttc gctgtccgtg aggcgttgga caagcgaagc gcgtcagttt
     3181 ttccggttct tacccgtgac aggtttttcc ggcagtttat cttccagtat cacaactttc
     3241 tgatacaccg ccgctgtggc gttgtagttc ctgtcgagca ttgttttcat tccccatccg
     3301 agcacacaga acataactag aaaagccact gacaccccac ccagccattt ccagctatac
     3361 cctttaaggg tgttgtattc ctgcacgata ttgctgtgaa ttgtcgcaat atccctgttc
     3421 agggcatccg cgtttgcctt catgtttgcg actgactctt ccagccgctg aaaatcctgg
     3481 ctgatgcgct cacctatcag ccctgctacg tccagattct gcacctcatt tctgacttca
     3541 tccctgacct gttttgccac accgtcagaa atatcccgat atccgcgaag tgtttccctt
     3601 gccacagcca gagaatcagc aacagctttc tgttgctcca ttgcggtatg ttgcaaatgc
     3661 tccaccgata acaggagcgt gagtaatttt tcttctgtgc tggccatgtt cagaatccca
     3721 tagaaaatcc acgtgacggt gatttaaccc gttcaggttc agttcggtcc tgtctgctca
     3781 ctctgtctct catgtcaaag ccatgctccc gcgcaacatc atgttgcagc cttctgacca
     3841 gttcatgttg cttaaccgga gtgtccagcc attccatgat gtcatgcccg accgttcgcg
     3901 cctgttcaag atggcgggaa tagtccggtt cagaccgttt ttgcatgatg tcatgacgct
     3961 gggcatcatc aagccgtccg gcagcttcaa aacccagatg cacttcggga cgtcgttcaa
     4021 tgccctgttc tttcagcgtc cgacagtcaa tgaagcccct ttcaaagcca ttttttctca
     4081 tatgttcgtt gtgcttcacc tcccagcgtt ttcgcaattc ccgcaactgc tctttttgct
     4141 gagtgggagt caggcttcct gaatcctttt tcgcacctcc ccgctcggga tacctggcgt
     4201 tatagcgccg gaaatactgt tccggtgtac gttcaatgcc atcattcaca cgctgactca
     4261 tcatgatatg cgcatgaggc tgttcgcctc cctcaatgct cgctttcgga ttgtgaatgg
     4321 caaaactcca tgcatgccgt tcgccagctt cctgacgtac aaaatcctgc accagttcaa
     4381 ggcgctgttc cggtgtcagt tcacggggca gcgctatctc cagttcccga taagtcgaac
     4441 cgtttgcacg ttcaaactgg tcagccgcct gccagaaatg ggagggatta tctcgcgccc
     4501 attccggcat gtttccgtgt gcggaatatt ccaaatcttg acggtcgcgg tatttctctt
     4561 tgcgctcgat gtaatcggca tggtttgcag cctgtccctt gccaccaaac ttaacgctga
     4621 ggtgataggt cgccacggtt tcagccctcc gcaggagcgc actgtttctc gacgaaggag
     4681 agaaacatat aagtgcgctt ccttctaatt ttacgaatta gcacggaaga taccccctgc
     4741 acccgccgac cgcaagcgat atactgcacg accacaacac accgcaaaaa aacaccaggg
     4801 agcaccacga acatgcgcat cagcaacatc gaatggctga agaaacggat cggattcatc
     4861 aggaagctcg gagaacagac cgcacgacag agacagatta ttgacctgct cgacaacgaa
     4921 gcaggactca cagaacagga acgcaaactc ctccacgtac tggcaacagc ggaaaaaaac
     4981 gacttacagg cacaggagag cgaacgaaaa caggcggtcc aaaaacgaat cgagggaaaa
     5041 aaacaacgac gagaacgcaa tcaccgactc tttctcgcgg ctgggctact gatcgaagca
     5101 ggactggtag acacaaaaac aggcgagcta tgctataaaa aagacaggat attacaggca
     5161 cttaaggaaa ttaagtacga tctggagaca tccccaaacc cagacgcgta agtgcctcca
     5221 tccgtgttcc ggttccctgg tgcttcctcg ctcattcgct cgctacgctc ggtcatgaga
     5281 ctgcggcgag cacgaccacc tcaatcatgc ggtaagccgc accattgctg ggctgagagg
     5341 agaaaaccgc cggaaagccc gctgctcaaa aagacgccga aaggcgctgt tatctgatat
     5401 aggcttgttc atgtccgcga atttaacatc ccattaacaa aaaacggggg ggtagtgttg
     5461 cttctttgtt aa
//
