LOCUS       NZ_CP061686             6078 bp    DNA     circular CON 10-JAN-2024
DEFINITION  Acinetobacter seifertii strain AS5 plasmid pAS5-3, complete
            sequence.
ACCESSION   NZ_CP061686
VERSION     NZ_CP061686.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN16092211
            Assembly: GCF_014723195.1
KEYWORDS    RefSeq.
SOURCE      Acinetobacter seifertii
  ORGANISM  Acinetobacter seifertii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Acinetobacter; Acinetobacter calcoaceticus/baumannii
            complex.
REFERENCE   1  (bases 1 to 6078)
  AUTHORS   Li,L.-H., Yang,Y.-S., Sun,J.-R., Huang,T.-W., Huang,W.-C.,
            Wang,Y.-C., Kuo,T.-H., Kuo,S.-C. and Chen,T.-L.
  TITLE     Clinical and molecular characterization of Acinetobacter seifertii
            in Taiwan
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6078)
  AUTHORS   Chen,F.-J. and Lee,Y.-T.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2020) Department of Emergency Medicine, Taipei
            Veterans General Hospital, No.201, Section 2, Shipai Road, Taipei
            112, Taiwan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 2020
            Assembly Method                   :: CCBGpipe v. 1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 80x
            Sequencing Technology             :: Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/10/2024 01:28:29
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,212
            CDSs (total)                      :: 4,116
            Genes (coding)                    :: 3,990
            CDSs (with protein)               :: 3,990
            Genes (RNA)                       :: 96
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 75 of 126
            Pseudo Genes (incomplete)         :: 49 of 126
            Pseudo Genes (internal stop)      :: 22 of 126
            Pseudo Genes (multiple problems)  :: 16 of 126
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP061686.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..6078
                     /organism="Acinetobacter seifertii"
                     /mol_type="genomic DNA"
                     /strain="AS5"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1530123"
                     /plasmid="pAS5-3"
                     /geo_loc_name="Taiwan"
                     /collection_date="2010/2017"
                     /collected_by="TVGH"
     gene            join(5868..6078,1..1604)
                     /locus_tag="IC764_RS20925"
                     /old_locus_tag="IC764_20915"
     CDS             join(5868..6078,1..1604)
                     /locus_tag="IC764_RS20925"
                     /old_locus_tag="IC764_20915"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="WP_000634474.1"
                     /translation="MIVDFFRHGSGLSKGCLDYLLGEDREREHAQVLNGDVELTAQLID
                     SSPFAKKYTSGCLSFYEHDLSDQDKQKIMQNFEECLFPGLDKDQYQILWVQHQDKINQD
                     TGETRLELNFVIPNVELSTGKRLQPFYAPVDLDRVDLFKQITNTEHSLYDPDDPEHRQL
                     FLNKKNLPKDIKDFKAQLHQRVYRAVANGDVADRQELVQWLESNQINVTRQVKNSISIE
                     NPYEGAKRPIRLEGEIYEQGFRATGEYRQEVQQRIEEYRGTTSERYRTNVTDYQRQLEH
                     KSQYHCDRYPTVGRENSPEHSKQRPDGREAVKPVSNLAAIEIEPFNAIKRANTEPRTTS
                     PESSGTEKTYHFEYGTDFSSSYFAYSNFLAWSRHQKQVQRDADAKYRDQREANEGRPFE
                     PIGRQFDDQHMQATRQQSTAPMYSDQQERRRMAERLHDSNGVLNDDRIRNTIIENHRRT
                     TATINATTSAIAEATASFRHNANQNYPSLVSTIKDNQERSAKLTASTEIISDNAEAISR
                     ARTKYSKLYRADQWQSPRDEPYHSQQRRNSGENLNHTAFCLVRSSQSAAEFARNIRDIE
                     KNLVQMNERKQEIEKPRPKQDRGMDFGM"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            1713..2198
                     /locus_tag="IC764_RS20930"
                     /old_locus_tag="IC764_20920"
     CDS             1713..2198
                     /locus_tag="IC764_RS20930"
                     /old_locus_tag="IC764_20920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005200081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_051064109.1"
                     /translation="MNNNCVVVLTARGKTRILKEGGSQSWRLNAHNAGKCDYLVCVQNR
                     KQSWGEPEADHHTAFLVGKISSISQSQEEDADGRWIINISEYADISIPNMWDGNRNPVS
                     YSSLEEMNIDLNELKFTKLNEDNSSKTLDKISIPEAKRLLANYFDVPQENISITINM"
                     /name="hypothetical protein"
     gene            2331..2999
                     /locus_tag="IC764_RS20935"
                     /old_locus_tag="IC764_20925"
     CDS             2331..2999
                     /locus_tag="IC764_RS20935"
                     /old_locus_tag="IC764_20925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000423610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Abi family protein"
                     /protein_id="WP_000423610.1"
                     /translation="MELSNDAVLKIEQALSIPRLSKYENFYKDKGEPYEKSDVLMLYER
                     NLIISNKFFYLLNYFEVVLRNAVVQAIEISFRCNETNSWHENEAFIRSLSRRGRYSPKS
                     MFDSAKEKFPDSPSKMIPELKFVFWQKMLMANYEERIWQGCFNSIFPNATVENRQIFYD
                     WTDQLRELRNRIAHHEPIIFNRNLENDLEVLTKFIYCRCINTYDFIEQSALDLKEYLDF
                     "
                     /name="Abi family protein"
     gene            complement(3158..3691)
                     /gene="aadB"
                     /locus_tag="IC764_RS20940"
                     /old_locus_tag="IC764_20930"
     CDS             complement(3158..3691)
                     /gene="aadB"
                     /locus_tag="IC764_RS20940"
                     /old_locus_tag="IC764_20930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000381802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase
                     ANT(2'')-Ia"
                     /protein_id="WP_010883707.1"
                     /translation="MDTTQVALIHQILAAADERNLPLWIGGGWAIDARLGRVTRKHDDI
                     DLTFPGERRGELEAIVEMLGGRVTEELDYGFLAEIGDELLDCEPAWWADEAYEIAEAPQ
                     GSCPEAAEGVIAGRPVRCNSWEAIIWDYFYYADEVPPVDWPTKHIESYRFACTSLGAEK
                     VEVLRAAFRSRYAA"
                     /name="aadB"
     gene            complement(3782..4468)
                     /locus_tag="IC764_RS20945"
                     /old_locus_tag="IC764_20935"
     CDS             complement(3782..4468)
                     /locus_tag="IC764_RS20945"
                     /old_locus_tag="IC764_20935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004986708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="WP_002011386.1"
                     /translation="MSKLPRFHNKHIEKRKYSNSLLAKSISSKVRFSNKTFFNVNLRGS
                     RLRSCSFKNIKIVLSDFSGVILNKNRFTNVHFKKCVFYAAIFRDCRFKNCTFEKCIFIN
                     TNTESLNHSILDCSKKYNFYEMKIPDDLKDQLTEYRNIPILQKNRLLHLKGGRINTATL
                     FILLSKISKYKLLNGLKKIYIEEQSKVKILSTFKLLEEIKNLVDTNTAFKQNNGALPHP
                     IERLDS"
                     /name="pentapeptide repeat-containing protein"
     gene            5471..5878
                     /locus_tag="IC764_RS20950"
                     /old_locus_tag="IC764_20940"
     CDS             5471..5878
                     /locus_tag="IC764_RS20950"
                     /old_locus_tag="IC764_20940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000016535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_000016535.1"
                     /translation="MSGQEQENKQSKAVRERTFKVRLSESEYQLLDSNNPYGSIAKLLR
                     QAAVEKATKIHNSKAEDDQKIKATKVVESSYSKTERALILELGRIGNNVNQIAKAVNTD
                     IAGVGTFDKVKLLHLLISIDQQLRELRPDDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     ARG             complement(3162..3691)
                     /name="ant(2'')-Ia"
                     /gene="ant(2'')-Ia"
                     /gene_name="aminoglycoside nucleotidyltransferase
                     ANT(2'')-Ia"
                     /tool="amrfinderplus"
                     /drug_class="AMINOGLYCOSIDE"
                     /identity="98.31"
                     /coverage="100.0"
     ARG             complement(3159..3703)
                     /name="ANT(2'')-Ia"
                     /gene="ANT(2'')-Ia"
                     /gene_name="ANT(2'')"
                     /tool="rgi"
                     /drug_class="aminoglycoside antibiotic"
                     /identity="97.74"
                     /coverage="102.26"
     Typing          4..1601
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          5869..6077
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="97.0"
                     /evalue="1.32e-38"
ORIGIN
        1 agaaaatcat gcaaaatttt gaggaatgtt tattcccggg actagataaa gaccaatatc
       61 aaattttatg ggtacagcac caagacaaaa ttaatcagga cacaggtgag acaaggcttg
      121 agcttaattt tgtgattcct aatgttgagc tttcaacagg caaacgccta cagccattct
      181 atgcgccagt cgatttagat cgagtcgatc tatttaagca gatcactaat accgaacatt
      241 cactgtatga ccccgatgat cctgagcatc gacagctatt tttaaataaa aagaacctac
      301 ccaaagatat taaggatttt aaagcgcagt tacatcaacg tgtttaccga gcggttgcca
      361 atggtgatgt tgccgatcgg caagaattgg tgcaatggct tgaatcgaat caaatcaatg
      421 tcactcgaca agtaaaaaac tccatatcca ttgaaaaccc atatgagggc gcaaaacgtc
      481 ccatccgttt agagggcgag atttatgagc aaggctttag agctactggc gaatatagac
      541 aagaagtaca acaacgaatt gaagaataca gaggaacaac ttctgagcgg tatcgaacaa
      601 acgtcacaga ctatcagaga cagcttgaac ataagagcca atatcactgc gaccgctatc
      661 caacagtcgg acgagaaaat agcccagagc attcaaaaca acgcccagac ggtcgagaag
      721 cagttaagcc tgtatcaaac cttgctgcaa tcgaaattga gccatttaac gcaattaaac
      781 gagcaaatac agagccaaga acaacaagcc cagaatcaag cggtacagaa aaaacttacc
      841 actttgaata tgggactgat ttcagtagca gttattttgc ttatagcaat tttctcgctt
      901 ggtctcgtca ccaaaaacaa gtacagcgag atgcagacgc taaataccgt gatcaacgag
      961 aagcaaatga gggtcgacca tttgaaccga tcgggaggca gtttgacgat cagcacatgc
     1021 aagcgaccag acagcaaagc acagcgccta tgtattcaga tcaacaagaa cgcaggcgaa
     1081 tggcagaacg gctacatgat tccaatgggg tattaaacga tgaccgaatt agaaacacaa
     1141 ttattgagaa tcatcgaaga acaacagcaa caattaatgc aacaacaagt gcaattgcag
     1201 aagcaacagc aagctttaga cacaatgcaa accaaaatta tccaagcctt gtcagcacaa
     1261 tcaaagacaa tcaagagcga tcagcaaagc ttacagcaag cacagaaatc attagcgaca
     1321 atgcagaagc aatttcacga gctagaacga agtacagcaa gctataccga gcagatcaat
     1381 ggcaaagtcc gagagatgag ccttatcaca gccaacaacg aagaaattct ggagagaatc
     1441 tcaaccacac agcattctgt ctcgtcagaa gtagccaaag cgcagcagaa tttgctcgaa
     1501 acatccgaga tattgaaaaa aatctcgtcc agatgaatga gcgtaaacaa gagatagaaa
     1561 aacctcgacc taagcaagat cgaggtatgg attttgggat gtaattattt tctagtatgt
     1621 tggtgtttat ttcttagtat tagcttgtac taatgtttaa atttagctat attgaatgta
     1681 gtataggctt gtttatatat tttggtggtg ttatgaataa taactgtgta gtagtactaa
     1741 ctgctcgtgg aaagactaga attttaaaag aaggtggtag tcagtcttgg agactaaatg
     1801 ctcataatgc aggaaagtgc gattatctag tttgtgtgca aaataggaaa caatcttggg
     1861 gagaaccaga ggctgatcat cacactgcat ttttagttgg caaaatatct agtatcagcc
     1921 aatctcaaga agaagatgca gatggtagat ggataataaa tatctccgaa tatgctgata
     1981 tatctattcc caatatgtgg gatggtaatc ggaatccagt ttcttatagt tcattagaag
     2041 agatgaatat cgatttgaat gagctgaaat ttaccaaatt aaatgaagat aattcttcta
     2101 aaacactgga taaaatatct attcctgaag ctaagagact attagctaat tattttgatg
     2161 tccctcaaga aaatatttca attacaatca atatgtaagc ttttaattga ccaaaaaaag
     2221 aaaaaagcct ttcacgttaa tgaaaggctt ttttgaatct tagcgcttgg gagggaagat
     2281 cttagatccg cagtctctca aacttacaac tatatattag ggtatatacg gtggaattgt
     2341 caaatgatgc agtgcttaag atagagcagg ctttatctat accacgctta tctaagtatg
     2401 aaaatttcta taaagataaa ggtgaaccat atgaaaaatc tgatgtattg atgctttatg
     2461 aacgtaattt aataatttcc aataagtttt tttacttatt aaattatttt gaagttgttc
     2521 tgagaaatgc tgttgttcag gcaatagaaa tatcttttag gtgtaatgaa acaaatagtt
     2581 ggcatgaaaa tgaagctttt attcgaagcc ttagccgtag agggaggtat agccctaaaa
     2641 gtatgtttga tagtgctaaa gagaagttcc cagattcgcc tagtaaaatg atccctgagc
     2701 ttaaatttgt attctggcaa aagatgctta tggctaatta tgaagaacgt atttggcagg
     2761 gatgttttaa ttctattttt cctaatgcaa cagttgaaaa caggcaaatt ttttacgatt
     2821 ggacagatca attacgtgaa ttaagaaaca ggattgctca tcatgaaccg attattttta
     2881 accgtaatct agaaaatgat ttagaggttt taacaaaatt tatttactgt cgttgtatta
     2941 atacatacga ttttatcgag caatctgcat tagatctaaa agaatattta gatttttaag
     3001 ataagaattt caccccaaac aatcgaaagg tcagactatt ttgcaaatgt tgaattaaca
     3061 aggttagtag taaagcagta aatccgataa cggtagcagt gtttcctaac acctgagtta
     3121 agccgcgccg caaagcggcg tcggcttgga cgaattgtta ggccgcatat cgcgacctga
     3181 aagcggcacg caagacctca accttttccg ccccgagtga ggtgcatgcg aacctgtagg
     3241 actctatgtg ctttgtaggc cagtccactg gtggtacttc atcggcatag taaaagtaat
     3301 cccagatgat cgcctcccag ctgttacaac ggactggccg cccggcgatg acaccctcag
     3361 ccgcctctgg gcacgagccc tgcggagcct ccgcgatttc atacgcttcg tctgcccacc
     3421 aagcaggttc gcagtcaagt aactcatccc cgatctccgc taagaatcca tagtccaact
     3481 cctccgtgac gcgcccgccg agcatttcaa ctattgcctc gagctcgccg cgcctctcgc
     3541 cgggaaaagt cagatcaata tcatcgtgct tgcgtgttac acgccctagc cgtgcatcga
     3601 tcgcccagcc cccaccgatc cagagcggca gatttcgctc atctgccgca gctagaattt
     3661 ggtgtatcaa tgcgacctgc gttgtgtcca tgcggcctaa catagtaagc cctcccagaa
     3721 tgtcagtaat tagactatta attgaattgt tacatccaat agtgagctcc cagtgattgt
     3781 cttaactatc caacctttcg attggatggg gcagagcacc attattctgt ttgaatgcgg
     3841 tgttagtgtc aactaggttc tttatttctt caaggagttt gaaagtgcta agtattttta
     3901 ctttactttg ttcctcaata taaatttttt taagaccatt tagcagcttg tatttggata
     3961 ttttggatag gagaatgaat aaggttgcgg tattgattct tccccctttt agatgaagta
     4021 aacggttttt ttgcagtatt ggaatatttc tatattctgt tagttgatct tttaagtcat
     4081 caggaatttt catttcgtaa aaattatatt ttttagaaca gtctaatatc gagtgattta
     4141 ggctttctgt atttgtatta ataaaaatgc atttttcaaa tgtgcaattt ttaaagcgac
     4201 aatctctaaa aatagcagca taaaatacgc actttttgaa gtgaacattc gtaaacctat
     4261 tcttatttaa gattactcct gaaaaatcag ataaaacaat ttttatattt ttgaatgaac
     4321 atgatcgtag gcgggatcct ctcaaattaa cattaaaaaa tgttttatta gagaaacgta
     4381 ctttagaaga aatagatttg gctaggaggc tatttgaata cttacgtttt tcgatgtgct
     4441 tgttatgaaa tctaggaagt tttgacatta atagtccaaa gaataggagt ttcagcttaa
     4501 ttcttaggtg taatcaaaaa aaataatgga caggtctctt gcgaaatctg tccattagtt
     4561 ttaaattcga tgggtacagt tatttgatgc ttgcaacatc aattaacgaa agatcggcag
     4621 agcttgcaac tcatactgat attcacctta aaggctagta ccactatcta ttttaaagaa
     4681 ttagaacagt ttgacaagcc tcaatttgaa gtccttcgtt aattcagcag tttaaatcat
     4741 caaataagca cctaacggtc gagctttatc tacttctgtg cgcctttttt aaggaagata
     4801 tgcgttgcat gtggagggaa taagtgtcac tggtgcatcg tagcgatagt cgcaaggcta
     4861 aaccgcttac aggggatctc acataggcgg agcgttggaa gtagttgtat aggttggtta
     4921 agttgcatgt tggagtaatc ctcgcagaga taccaccata cttcgacagg gagcgcgtgg
     4981 gagaaggtgc aaaaccttta gtccttgtct gcatggtcag caaaaccatg tgcggatacg
     5041 accgatgcgt ggctccgtca gcccaattac aggttaatcg gcaatactta agaggatatt
     5101 tgattttttt aaatcaaagc ctcttaggga aagtattgcc gaaactctct caacgttcac
     5161 catcagcccg agcaaagcga gggatgtggg gacggtgaga aagagttgag cgcatttgtt
     5221 tttacaagat tcatgccaat acctactaga gtttatatac cgtgtcattt gattagtttt
     5281 tattgaattt cactcgtcca tttttaaata agcaaggata cacagctacc cctatttaac
     5341 ttcgttaaac agtgatagtc tgtgaccttg atcaggggct tcacccccga tacccccgaa
     5401 aagctttaaa cagaaaaatc aaagattttc caatttaaag cttacaaaag ccaaagcagg
     5461 gtagattgaa atgtcaggac aggaacaaga aaacaaacag agcaaagcag ttcgagaacg
     5521 aacctttaaa gtcagacttt cagaatcaga atatcaattg ctcgattcca ataatcctta
     5581 tggttcgatt gcaaagttgc ttagacaagc agcagtagag aaagcgacta aaattcataa
     5641 ttccaaagca gaagatgacc aaaaaattaa agcaacaaag gttgttgaat cttcgtatag
     5701 caagacagag cgagcattaa ttttagagct tggtcggatt ggcaacaatg tgaatcagat
     5761 tgctaaagca gtgaataccg atattgcagg ggtaggtact tttgacaaag tgaagttact
     5821 tcatttgttg atttccatag atcagcagct tcgggagtta cgtccagatg atcgttgatt
     5881 tctttagaca tggttcaggt ttatcaaaag gctgtctcga ttatttgtta ggtgaagatc
     5941 gagagcgtga acatgctcaa gtgctcaatg gtgatgtaga actgacagca caactcattg
     6001 atagcagtcc ttttgccaaa aaatatacca gtggttgttt gtccttttat gagcatgatt
     6061 tatctgacca ggataaac
//
