LOCUS       NZ_CP061680             6077 bp    DNA     circular CON 10-JAN-2024
DEFINITION  Acinetobacter seifertii strain AS11 plasmid pAS11-2, complete
            sequence.
ACCESSION   NZ_CP061680
VERSION     NZ_CP061680.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN16092214
            Assembly: GCF_014723135.1
KEYWORDS    RefSeq.
SOURCE      Acinetobacter seifertii
  ORGANISM  Acinetobacter seifertii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Acinetobacter; Acinetobacter calcoaceticus/baumannii
            complex.
REFERENCE   1  (bases 1 to 6077)
  AUTHORS   Li,L.-H., Yang,Y.-S., Sun,J.-R., Huang,T.-W., Huang,W.-C.,
            Wang,Y.-C., Kuo,T.-H., Kuo,S.-C. and Chen,T.-L.
  TITLE     Clinical and molecular characterization of Acinetobacter seifertii
            in Taiwan
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6077)
  AUTHORS   Chen,F.-J. and Lee,Y.-T.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2020) Department of Emergency Medicine, Taipei
            Veterans General Hospital, No.201, Section 2, Shipai Road, Taipei
            112, Taiwan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 2020
            Assembly Method                   :: CCBGpipe v. 1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 80x
            Sequencing Technology             :: Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/10/2024 01:04:02
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,146
            CDSs (total)                      :: 4,050
            Genes (coding)                    :: 3,926
            CDSs (with protein)               :: 3,926
            Genes (RNA)                       :: 96
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 78 of 124
            Pseudo Genes (incomplete)         :: 47 of 124
            Pseudo Genes (internal stop)      :: 21 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP061680.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..6077
                     /organism="Acinetobacter seifertii"
                     /mol_type="genomic DNA"
                     /strain="AS11"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1530123"
                     /plasmid="pAS11-2"
                     /geo_loc_name="Taiwan"
                     /collection_date="2010/2017"
                     /collected_by="TVGH"
     gene            complement(join(4553..6077,1..290))
                     /locus_tag="IC767_RS20585"
                     /old_locus_tag="IC767_20575"
     CDS             complement(join(4553..6077,1..290))
                     /locus_tag="IC767_RS20585"
                     /old_locus_tag="IC767_20575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000634474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="WP_000634474.1"
                     /translation="MIVDFFRHGSGLSKGCLDYLLGEDREREHAQVLNGDVELTAQLID
                     SSPFAKKYTSGCLSFYEHDLSDQDKQKIMQNFEECLFPGLDKDQYQILWVQHQDKINQD
                     TGETRLELNFVIPNVELSTGKRLQPFYAPVDLDRVDLFKQITNTEHSLYDPDDPEHRQL
                     FLNKKNLPKDIKDFKAQLHQRVYRAVANGDVADRQELVQWLESNQINVTRQVKNSISIE
                     NPYEGAKRPIRLEGEIYEQGFRATGEYRQEVQQRIEEYRGTTSERYRTNVTDYQRQLEH
                     KSQYHCDRYPTVGRENSPEHSKQRPDGREAVKPVSNLAAIEIEPFNAIKRANTEPRTTS
                     PESSGTEKTYHFEYGTDFSSSYFAYSNFLAWSRHQKQVQRDADAKYRDQREANEGRPFE
                     PIGRQFDDQHMQATRQQSTAPMYSDQQERRRMAERLHDSNGVLNDDRIRNTIIENHRRT
                     TATINATTSAIAEATASFRHNANQNYPSLVSTIKDNQERSAKLTASTEIISDNAEAISR
                     ARTKYSKLYRADQWQSPRDEPYHSQQRRNSGENLNHTAFCLVRSSQSAAEFARNIRDIE
                     KNLVQMNERKQEIEKPRPKQDRGMDFGM"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(280..687)
                     /locus_tag="IC767_RS20590"
                     /old_locus_tag="IC767_20580"
     CDS             complement(280..687)
                     /locus_tag="IC767_RS20590"
                     /old_locus_tag="IC767_20580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000016535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_000016535.1"
                     /translation="MSGQEQENKQSKAVRERTFKVRLSESEYQLLDSNNPYGSIAKLLR
                     QAAVEKATKIHNSKAEDDQKIKATKVVESSYSKTERALILELGRIGNNVNQIAKAVNTD
                     IAGVGTFDKVKLLHLLISIDQQLRELRPDDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            1690..2376
                     /locus_tag="IC767_RS20595"
                     /old_locus_tag="IC767_20585"
     CDS             1690..2376
                     /locus_tag="IC767_RS20595"
                     /old_locus_tag="IC767_20585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004986708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="WP_002011386.1"
                     /translation="MSKLPRFHNKHIEKRKYSNSLLAKSISSKVRFSNKTFFNVNLRGS
                     RLRSCSFKNIKIVLSDFSGVILNKNRFTNVHFKKCVFYAAIFRDCRFKNCTFEKCIFIN
                     TNTESLNHSILDCSKKYNFYEMKIPDDLKDQLTEYRNIPILQKNRLLHLKGGRINTATL
                     FILLSKISKYKLLNGLKKIYIEEQSKVKILSTFKLLEEIKNLVDTNTAFKQNNGALPHP
                     IERLDS"
                     /name="pentapeptide repeat-containing protein"
     gene            2467..3000
                     /gene="aadB"
                     /locus_tag="IC767_RS20600"
                     /old_locus_tag="IC767_20590"
     CDS             2467..3000
                     /gene="aadB"
                     /locus_tag="IC767_RS20600"
                     /old_locus_tag="IC767_20590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000381802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase
                     ANT(2'')-Ia"
                     /protein_id="WP_010883707.1"
                     /translation="MDTTQVALIHQILAAADERNLPLWIGGGWAIDARLGRVTRKHDDI
                     DLTFPGERRGELEAIVEMLGGRVTEELDYGFLAEIGDELLDCEPAWWADEAYEIAEAPQ
                     GSCPEAAEGVIAGRPVRCNSWEAIIWDYFYYADEVPPVDWPTKHIESYRFACTSLGAEK
                     VEVLRAAFRSRYAA"
                     /name="aadB"
     gene            complement(3159..3827)
                     /locus_tag="IC767_RS20605"
                     /old_locus_tag="IC767_20595"
     CDS             complement(3159..3827)
                     /locus_tag="IC767_RS20605"
                     /old_locus_tag="IC767_20595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000423610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Abi family protein"
                     /protein_id="WP_000423610.1"
                     /translation="MELSNDAVLKIEQALSIPRLSKYENFYKDKGEPYEKSDVLMLYER
                     NLIISNKFFYLLNYFEVVLRNAVVQAIEISFRCNETNSWHENEAFIRSLSRRGRYSPKS
                     MFDSAKEKFPDSPSKMIPELKFVFWQKMLMANYEERIWQGCFNSIFPNATVENRQIFYD
                     WTDQLRELRNRIAHHEPIIFNRNLENDLEVLTKFIYCRCINTYDFIEQSALDLKEYLDF
                     "
                     /name="Abi family protein"
     gene            complement(3959..4444)
                     /locus_tag="IC767_RS20610"
                     /old_locus_tag="IC767_20600"
     CDS             complement(3959..4444)
                     /locus_tag="IC767_RS20610"
                     /old_locus_tag="IC767_20600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005200081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_051064109.1"
                     /translation="MNNNCVVVLTARGKTRILKEGGSQSWRLNAHNAGKCDYLVCVQNR
                     KQSWGEPEADHHTAFLVGKISSISQSQEEDADGRWIINISEYADISIPNMWDGNRNPVS
                     YSSLEEMNIDLNELKFTKLNEDNSSKTLDKISIPEAKRLLANYFDVPQENISITINM"
                     /name="hypothetical protein"
     ARG             2468..2997
                     /name="ant(2'')-Ia"
                     /gene="ant(2'')-Ia"
                     /gene_name="aminoglycoside nucleotidyltransferase
                     ANT(2'')-Ia"
                     /tool="amrfinderplus"
                     /drug_class="AMINOGLYCOSIDE"
                     /identity="98.31"
                     /coverage="100.0"
     ARG             2456..3000
                     /name="ANT(2'')-Ia"
                     /gene="ANT(2'')-Ia"
                     /gene_name="ANT(2'')"
                     /tool="rgi"
                     /drug_class="aminoglycoside antibiotic"
                     /identity="97.74"
                     /coverage="102.26"
     Typing          complement(4..290)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="97.0"
                     /evalue="5.05e-58"
     Typing          complement(4557..6076)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 tgctgtaccc ataaaatttg atattggtct ttatctagtc ccgggaataa acattcctca
       61 aaattttgca tgattttctg tttatcctgg tcagataaat catgctcata aaaggacaaa
      121 caaccactgg tatatttttt ggcaaaagga ctgctatcaa tgagttgtgc tgtcagttct
      181 acatcaccat tgagcacttg agcatgttca cgctctcgat cttcacctaa caaataatcg
      241 agacagcctt ttgataaacc tgaaccatgt ctaaagaaat caacgatcat ctggacgtaa
      301 ctcccgaagc tgctgatcta tggaaatcaa caaatgaagt aacttcactt tgtcaaaagt
      361 acctacccct gcaatatcgg tattcactgc tttagcaatc tgattcacat tgttgccaat
      421 ccgaccaagc tctaaaatta atgctcgctc tgtcttgcta tacgaagatt caacaacctt
      481 tgttgcttta attttttggt catcttctgc tttggaatta tgaattttag tcgctttctc
      541 tactgctgct tgtctaagca actttgcaat cgaaccataa ggattattgg aatcgagcaa
      601 ttgatattct gattctgaaa gtctgacttt aaaggttcgt tctcgaactg ctttgctctg
      661 tttgttttct tgttcctgtc ctgacatttc aatctaccct gctttggctt ttgtaagctt
      721 taaattggaa aatctttgat ttttctgttt aaagcttttc gggggtatcg ggggtgaagc
      781 ccctgatcaa ggtcacagac tatcactgtt taacgaagtt aaataggggt agctgtgtat
      841 ccttgcttat ttaaaaatgg acgagtgaaa ttcaataaaa actaatcaaa tgacacggta
      901 tataaactct agtaggtatt ggcatgaatc ttgtaaaaac aaatgcgctc aactctttct
      961 caccgtcccc acatccctcg ctttgctcgg gctgatggtg aacgttgaga gagtttcggc
     1021 aatactttcc ctaagaggct ttgatttaaa aaaatcaaat atcctcttaa gtattgccga
     1081 ttaacctgta attgggctga cggagccacg catcggtcgt atccgcacat ggttttgctg
     1141 accatgcaga caaggactaa aggttttgca ccttctccca cgcgctccct gtcgaagtat
     1201 ggtggtatct ctgcgaggat tactccaaca tgcaacttaa ccaacctata caactacttc
     1261 caacgctccg cctatgtgag atcccctgta agcggtttag ccttgcgact atcgctacga
     1321 tgcaccagtg acacttattc cctccacatg caacgcatat cttccttaaa aaaggcgcac
     1381 agaagtagat aaagctcgac cgttaggtgc ttatttgatg atttaaactg ctgaattaac
     1441 gaaggacttc aaattgaggc ttgtcaaact gttctaattc tttaaaatag atagtggtac
     1501 tagcctttaa ggtgaatatc agtatgagtt gcaagctctg ccgatctttc gttaattgat
     1561 gttgcaagca tcaaataact gtacccatcg aatttaaaac taatggacag atttcgcaag
     1621 agacctgtcc attatttttt ttgattacac ctaagaatta agctgaaact cctattcttt
     1681 ggactattaa tgtcaaaact tcctagattt cataacaagc acatcgaaaa acgtaagtat
     1741 tcaaatagcc tcctagccaa atctatttct tctaaagtac gtttctctaa taaaacattt
     1801 tttaatgtta atttgagagg atcccgccta cgatcatgtt cattcaaaaa tataaaaatt
     1861 gttttatctg atttttcagg agtaatctta aataagaata ggtttacgaa tgttcacttc
     1921 aaaaagtgcg tattttatgc tgctattttt agagattgtc gctttaaaaa ttgcacattt
     1981 gaaaaatgca tttttattaa tacaaataca gaaagcctaa atcactcgat attagactgt
     2041 tctaaaaaat ataattttta cgaaatgaaa attcctgatg acttaaaaga tcaactaaca
     2101 gaatatagaa atattccaat actgcaaaaa aaccgtttac ttcatctaaa agggggaaga
     2161 atcaataccg caaccttatt cattctccta tccaaaatat ccaaatacaa gctgctaaat
     2221 ggtcttaaaa aaatttatat tgaggaacaa agtaaagtaa aaatacttag cactttcaaa
     2281 ctccttgaag aaataaagaa cctagttgac actaacaccg cattcaaaca gaataatggt
     2341 gctctgcccc atccaatcga aaggttggat agttaagaca atcactggga gctcactatt
     2401 ggatgtaaca attcaattaa tagtctaatt actgacattc tgggagggct tactatgtta
     2461 ggccgcatgg acacaacgca ggtcgcattg atacaccaaa ttctagctgc ggcagatgag
     2521 cgaaatctgc cgctctggat cggtgggggc tgggcgatcg atgcacggct agggcgtgta
     2581 acacgcaagc acgatgatat tgatctgact tttcccggcg agaggcgcgg cgagctcgag
     2641 gcaatagttg aaatgctcgg cgggcgcgtc acggaggagt tggactatgg attcttagcg
     2701 gagatcgggg atgagttact tgactgcgaa cctgcttggt gggcagacga agcgtatgaa
     2761 atcgcggagg ctccgcaggg ctcgtgccca gaggcggctg agggtgtcat cgccgggcgg
     2821 ccagtccgtt gtaacagctg ggaggcgatc atctgggatt acttttacta tgccgatgaa
     2881 gtaccaccag tggactggcc tacaaagcac atagagtcct acaggttcgc atgcacctca
     2941 ctcggggcgg aaaaggttga ggtcttgcgt gccgctttca ggtcgcgata tgcggcctaa
     3001 caattcgtcc aagccgacgc cgctttgcgg cgcggcttaa ctcaggtgtt aggaaacact
     3061 gctaccgtta tcggatttac tgctttacta ctaaccttgt taattcaaca tttgcaaaat
     3121 agtctgacct ttcgattgtt tggggtgaaa ttcttatctt aaaaatctaa atattctttt
     3181 agatctaatg cagattgctc gataaaatcg tatgtattaa tacaacgaca gtaaataaat
     3241 tttgttaaaa cctctaaatc attttctaga ttacggttaa aaataatcgg ttcatgatga
     3301 gcaatcctgt ttcttaattc acgtaattga tctgtccaat cgtaaaaaat ttgcctgttt
     3361 tcaactgttg cattaggaaa aatagaatta aaacatccct gccaaatacg ttcttcataa
     3421 ttagccataa gcatcttttg ccagaataca aatttaagct cagggatcat tttactaggc
     3481 gaatctggga acttctcttt agcactatca aacatacttt tagggctata cctccctcta
     3541 cggctaaggc ttcgaataaa agcttcattt tcatgccaac tatttgtttc attacaccta
     3601 aaagatattt ctattgcctg aacaacagca tttctcagaa caacttcaaa ataatttaat
     3661 aagtaaaaaa acttattgga aattattaaa ttacgttcat aaagcatcaa tacatcagat
     3721 ttttcatatg gttcaccttt atctttatag aaattttcat acttagataa gcgtggtata
     3781 gataaagcct gctctatctt aagcactgca tcatttgaca attccaccgt atatacccta
     3841 atatatagtt gtaagtttga gagactgcgg atctaagatc ttccctccca agcgctaaga
     3901 ttcaaaaaag cctttcatta acgtgaaagg cttttttttt ttttggtcaa ttaaaagctt
     3961 acatattgat tgtaattgaa atattttctt gagggacatc aaaataatta gctaatagtc
     4021 tcttagcttc aggaatagat attttatcca gtgttttaga agaattatct tcatttaatt
     4081 tggtaaattt cagctcattc aaatcgatat tcatctcttc taatgaacta taagaaactg
     4141 gattccgatt accatcccac atattgggaa tagatatatc agcatattcg gagatattta
     4201 ttatccatct accatctgca tcttcttctt gagattggct gatactagat attttgccaa
     4261 ctaaaaatgc agtgtgatga tcagcctctg gttctcccca agattgtttc ctattttgca
     4321 cacaaactag ataatcgcac tttcctgcat tatgagcatt tagtctccaa gactgactac
     4381 caccttcttt taaaattcta gtctttccac gagcagttag tactactaca cagttattat
     4441 tcataacacc accaaaatat ataaacaagc ctatactaca ttcaatatag ctaaatttaa
     4501 acattagtac aagctaatac taagaaataa acaccaacat actagaaaat aattacatcc
     4561 caaaatccat acctcgatct tgcttaggtc gaggtttttc tatctcttgt ttacgctcat
     4621 tcatctggac gagatttttt tcaatatctc ggatgtttcg agcaaattct gctgcgcttt
     4681 ggctacttct gacgagacag aatgctgtgt ggttgagatt ctctccagaa tttcttcgtt
     4741 gttggctgtg ataaggctca tctctcggac tttgccattg atctgctcgg tatagcttgc
     4801 tgtacttcgt tctagctcgt gaaattgctt ctgcattgtc gctaatgatt tctgtgcttg
     4861 ctgtaagctt tgctgatcgc tcttgattgt ctttgattgt gctgacaagg cttggataat
     4921 tttggtttgc attgtgtcta aagcttgctg ttgcttctgc aattgcactt gttgttgcat
     4981 taattgttgc tgttgttctt cgatgattct caataattgt gtttctaatt cggtcatcgt
     5041 ttaatacccc attggaatca tgtagccgtt ctgccattcg cctgcgttct tgttgatctg
     5101 aatacatagg cgctgtgctt tgctgtctgg tcgcttgcat gtgctgatcg tcaaactgcc
     5161 tcccgatcgg ttcaaatggt cgaccctcat ttgcttctcg ttgatcacgg tatttagcgt
     5221 ctgcatctcg ctgtacttgt ttttggtgac gagaccaagc gagaaaattg ctataagcaa
     5281 aataactgct actgaaatca gtcccatatt caaagtggta agttttttct gtaccgcttg
     5341 attctgggct tgttgttctt ggctctgtat ttgctcgttt aattgcgtta aatggctcaa
     5401 tttcgattgc agcaaggttt gatacaggct taactgcttc tcgaccgtct gggcgttgtt
     5461 ttgaatgctc tgggctattt tctcgtccga ctgttggata gcggtcgcag tgatattggc
     5521 tcttatgttc aagctgtctc tgatagtctg tgacgtttgt tcgataccgc tcagaagttg
     5581 ttcctctgta ttcttcaatt cgttgttgta cttcttgtct atattcgcca gtagctctaa
     5641 agccttgctc ataaatctcg ccctctaaac ggatgggacg ttttgcgccc tcatatgggt
     5701 tttcaatgga tatggagttt tttacttgtc gagtgacatt gatttgattc gattcaagcc
     5761 attgcaccaa ttcttgccga tcggcaacat caccattggc aaccgctcgg taaacacgtt
     5821 gatgtaactg cgctttaaaa tccttaatat ctttgggtag gttcttttta tttaaaaata
     5881 gctgtcgatg ctcaggatca tcggggtcat acagtgaatg ttcggtatta gtgatctgct
     5941 taaatagatc gactcgatct aaatcgactg gcgcatagaa tggctgtagg cgtttgcctg
     6001 ttgaaagctc aacattagga atcacaaaat taagctcaag ccttgtctca cctgtgtcct
     6061 gattaatttt gtcttgg
//
