LOCUS       NZ_CP061668             6079 bp    DNA     circular CON 10-JAN-2024
DEFINITION  Acinetobacter seifertii strain AS25 plasmid pAS25-2, complete
            sequence.
ACCESSION   NZ_CP061668
VERSION     NZ_CP061668.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN16092218
            Assembly: GCF_014723055.1
KEYWORDS    RefSeq.
SOURCE      Acinetobacter seifertii
  ORGANISM  Acinetobacter seifertii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Acinetobacter; Acinetobacter calcoaceticus/baumannii
            complex.
REFERENCE   1  (bases 1 to 6079)
  AUTHORS   Li,L.-H., Yang,Y.-S., Sun,J.-R., Huang,T.-W., Huang,W.-C.,
            Wang,Y.-C., Kuo,T.-H., Kuo,S.-C. and Chen,T.-L.
  TITLE     Clinical and molecular characterization of Acinetobacter seifertii
            in Taiwan
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6079)
  AUTHORS   Chen,F.-J. and Lee,Y.-T.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2020) Department of Emergency Medicine, Taipei
            Veterans General Hospital, No.201, Section 2, Shipai Road, Taipei
            112, Taiwan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 2020
            Assembly Method                   :: CCBGpipe v. 1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 80x
            Sequencing Technology             :: Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/10/2024 01:11:23
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,118
            CDSs (total)                      :: 4,021
            Genes (coding)                    :: 3,875
            CDSs (with protein)               :: 3,875
            Genes (RNA)                       :: 97
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 146
            CDSs (without protein)            :: 146
            Pseudo Genes (ambiguous residues) :: 0 of 146
            Pseudo Genes (frameshifted)       :: 84 of 146
            Pseudo Genes (incomplete)         :: 61 of 146
            Pseudo Genes (internal stop)      :: 22 of 146
            Pseudo Genes (multiple problems)  :: 17 of 146
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP061668.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..6079
                     /organism="Acinetobacter seifertii"
                     /mol_type="genomic DNA"
                     /strain="AS25"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1530123"
                     /plasmid="pAS25-2"
                     /geo_loc_name="Taiwan"
                     /collection_date="2010/2017"
                     /collected_by="TVGH"
     gene            complement(join(6041..6079,1..447))
                     /locus_tag="IC771_RS20410"
                     /old_locus_tag="IC771_20400"
     CDS             complement(join(6041..6079,1..447))
                     /locus_tag="IC771_RS20410"
                     /old_locus_tag="IC771_20400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005200081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_051064109.1"
                     /translation="MNNNCVVVLTARGKTRILKEGGSQSWRLNAHNAGKCDYLVCVQNR
                     KQSWGEPEADHHTAFLVGKISSISQSQEEDADGRWIINISEYADISIPNMWDGNRNPVS
                     YSSLEEMNIDLNELKFTKLNEDNSSKTLDKISIPEAKRLLANYFDVPQENISITINM"
                     /name="hypothetical protein"
     gene            complement(556..2370)
                     /locus_tag="IC771_RS20415"
                     /old_locus_tag="IC771_20405"
     CDS             complement(556..2370)
                     /locus_tag="IC771_RS20415"
                     /old_locus_tag="IC771_20405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000634474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="WP_000634474.1"
                     /translation="MIVDFFRHGSGLSKGCLDYLLGEDREREHAQVLNGDVELTAQLID
                     SSPFAKKYTSGCLSFYEHDLSDQDKQKIMQNFEECLFPGLDKDQYQILWVQHQDKINQD
                     TGETRLELNFVIPNVELSTGKRLQPFYAPVDLDRVDLFKQITNTEHSLYDPDDPEHRQL
                     FLNKKNLPKDIKDFKAQLHQRVYRAVANGDVADRQELVQWLESNQINVTRQVKNSISIE
                     NPYEGAKRPIRLEGEIYEQGFRATGEYRQEVQQRIEEYRGTTSERYRTNVTDYQRQLEH
                     KSQYHCDRYPTVGRENSPEHSKQRPDGREAVKPVSNLAAIEIEPFNAIKRANTEPRTTS
                     PESSGTEKTYHFEYGTDFSSSYFAYSNFLAWSRHQKQVQRDADAKYRDQREANEGRPFE
                     PIGRQFDDQHMQATRQQSTAPMYSDQQERRRMAERLHDSNGVLNDDRIRNTIIENHRRT
                     TATINATTSAIAEATASFRHNANQNYPSLVSTIKDNQERSAKLTASTEIISDNAEAISR
                     ARTKYSKLYRADQWQSPRDEPYHSQQRRNSGENLNHTAFCLVRSSQSAAEFARNIRDIE
                     KNLVQMNERKQEIEKPRPKQDRGMDFGM"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(2360..2767)
                     /locus_tag="IC771_RS20420"
                     /old_locus_tag="IC771_20410"
     CDS             complement(2360..2767)
                     /locus_tag="IC771_RS20420"
                     /old_locus_tag="IC771_20410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000016535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_000016535.1"
                     /translation="MSGQEQENKQSKAVRERTFKVRLSESEYQLLDSNNPYGSIAKLLR
                     QAAVEKATKIHNSKAEDDQKIKATKVVESSYSKTERALILELGRIGNNVNQIAKAVNTD
                     IAGVGTFDKVKLLHLLISIDQQLRELRPDDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            3770..4456
                     /locus_tag="IC771_RS20425"
                     /old_locus_tag="IC771_20415"
     CDS             3770..4456
                     /locus_tag="IC771_RS20425"
                     /old_locus_tag="IC771_20415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004986708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="WP_002011386.1"
                     /translation="MSKLPRFHNKHIEKRKYSNSLLAKSISSKVRFSNKTFFNVNLRGS
                     RLRSCSFKNIKIVLSDFSGVILNKNRFTNVHFKKCVFYAAIFRDCRFKNCTFEKCIFIN
                     TNTESLNHSILDCSKKYNFYEMKIPDDLKDQLTEYRNIPILQKNRLLHLKGGRINTATL
                     FILLSKISKYKLLNGLKKIYIEEQSKVKILSTFKLLEEIKNLVDTNTAFKQNNGALPHP
                     IERLDS"
                     /name="pentapeptide repeat-containing protein"
     gene            4547..5080
                     /gene="aadB"
                     /locus_tag="IC771_RS20430"
                     /old_locus_tag="IC771_20420"
     CDS             4547..5080
                     /gene="aadB"
                     /locus_tag="IC771_RS20430"
                     /old_locus_tag="IC771_20420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000381802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase
                     ANT(2'')-Ia"
                     /protein_id="WP_010883707.1"
                     /translation="MDTTQVALIHQILAAADERNLPLWIGGGWAIDARLGRVTRKHDDI
                     DLTFPGERRGELEAIVEMLGGRVTEELDYGFLAEIGDELLDCEPAWWADEAYEIAEAPQ
                     GSCPEAAEGVIAGRPVRCNSWEAIIWDYFYYADEVPPVDWPTKHIESYRFACTSLGAEK
                     VEVLRAAFRSRYAA"
                     /name="aadB"
     gene            complement(5239..5907)
                     /locus_tag="IC771_RS20435"
                     /old_locus_tag="IC771_20425"
     CDS             complement(5239..5907)
                     /locus_tag="IC771_RS20435"
                     /old_locus_tag="IC771_20425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000423610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Abi family protein"
                     /protein_id="WP_000423610.1"
                     /translation="MELSNDAVLKIEQALSIPRLSKYENFYKDKGEPYEKSDVLMLYER
                     NLIISNKFFYLLNYFEVVLRNAVVQAIEISFRCNETNSWHENEAFIRSLSRRGRYSPKS
                     MFDSAKEKFPDSPSKMIPELKFVFWQKMLMANYEERIWQGCFNSIFPNATVENRQIFYD
                     WTDQLRELRNRIAHHEPIIFNRNLENDLEVLTKFIYCRCINTYDFIEQSALDLKEYLDF
                     "
                     /name="Abi family protein"
     ARG             4548..5077
                     /name="ant(2'')-Ia"
                     /gene="ant(2'')-Ia"
                     /gene_name="aminoglycoside nucleotidyltransferase
                     ANT(2'')-Ia"
                     /tool="amrfinderplus"
                     /drug_class="AMINOGLYCOSIDE"
                     /identity="98.31"
                     /coverage="100.0"
     ARG             4536..5080
                     /name="ANT(2'')-Ia"
                     /gene="ANT(2'')-Ia"
                     /gene_name="ANT(2'')"
                     /tool="rgi"
                     /drug_class="aminoglycoside antibiotic"
                     /identity="97.74"
                     /coverage="102.26"
     Typing          complement(560..2370)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 atcaaaataa ttagctaata gtctcttagc ttcaggaata gatattttat ccagtgtttt
       61 agaagaatta tcttcattta atttggtaaa tttcagctca ttcaaatcga tattcatctc
      121 ttctaatgaa ctataagaaa ctggattccg attaccatcc cacatattgg gaatagatat
      181 atcagcatat tcggagatat ttattatcca tctaccatct gcatcttctt cttgagattg
      241 gctgatacta gatattttgc caactaaaaa tgcagtgtga tgatcagcct ctggttctcc
      301 ccaagattgt ttcctatttt gcacacaaac tagataatcg cactttcctg cattatgagc
      361 atttagtctc caagactgac taccaccttc ttttaaaatt ctagtctttc cacgagcagt
      421 tagtactact acacagttat tattcataac accaccaaaa tatataaaca agcctatact
      481 acattcaata tagctaaatt taaacattag tacaagctaa tactaagaaa taaacaccaa
      541 catactagaa aataattaca tcccaaaatc catacctcga tcttgcttag gtcgaggttt
      601 ttctatctct tgtttacgct cattcatctg gacgagattt ttttcaatat ctcggatgtt
      661 tcgagcaaat tctgctgcgc tttggctact tctgacgaga cagaatgctg tgtggttgag
      721 attctctcca gaatttcttc gttgttggct gtgataaggc tcatctctcg gactttgcca
      781 ttgatctgct cggtatagct tgctgtactt cgttctagct cgtgaaattg cttctgcatt
      841 gtcgctaatg atttctgtgc ttgctgtaag ctttgctgat cgctcttgat tgtctttgat
      901 tgtgctgaca aggcttggat aattttggtt tgcattgtgt ctaaagcttg ctgttgcttc
      961 tgcaattgca cttgttgttg cattaattgt tgctgttgtt cttcgatgat tctcaataat
     1021 tgtgtttcta attcggtcat cgtttaatac cccattggaa tcatgtagcc gttctgccat
     1081 tcgcctgcgt tcttgttgat ctgaatacat aggcgctgtg ctttgctgtc tggtcgcttg
     1141 catgtgctga tcgtcaaact gcctcccgat cggttcaaat ggtcgaccct catttgcttc
     1201 tcgttgatca cggtatttag cgtctgcatc tcgctgtact tgtttttggt gacgagacca
     1261 agcgagaaaa ttgctataag caaaataact gctactgaaa tcagtcccat attcaaagtg
     1321 gtaagttttt tctgtaccgc ttgattctgg gcttgttgtt cttggctctg tatttgctcg
     1381 tttaattgcg ttaaatggct caatttcgat tgcagcaagg tttgatacag gcttaactgc
     1441 ttctcgaccg tctgggcgtt gttttgaatg ctctgggcta ttttctcgtc cgactgttgg
     1501 atagcggtcg cagtgatatt ggctcttatg ttcaagctgt ctctgatagt ctgtgacgtt
     1561 tgttcgatac cgctcagaag ttgttcctct gtattcttca attcgttgtt gtacttcttg
     1621 tctatattcg ccagtagctc taaagccttg ctcataaatc tcgccctcta aacggatggg
     1681 acgttttgcg ccctcatatg ggttttcaat ggatatggag ttttttactt gtcgagtgac
     1741 attgatttga ttcgattcaa gccattgcac caattcttgc cgatcggcaa catcaccatt
     1801 ggcaaccgct cggtaaacac gttgatgtaa ctgcgcttta aaatccttaa tatctttggg
     1861 taggttcttt ttatttaaaa atagctgtcg atgctcagga tcatcggggt catacagtga
     1921 atgttcggta ttagtgatct gcttaaatag atcgactcga tctaaatcga ctggcgcata
     1981 gaatggctgt aggcgtttgc ctgttgaaag ctcaacatta ggaatcacaa aattaagctc
     2041 aagccttgtc tcacctgtgt cctgattaat tttgtcttgg tgctgtaccc ataaaatttg
     2101 atattggtct ttatctagtc ccgggaataa acattcctca aaattttgca tgattttctg
     2161 tttatcctgg tcagataaat catgctcata aaaggacaaa caaccactgg tatatttttt
     2221 ggcaaaagga ctgctatcaa tgagttgtgc tgtcagttct acatcaccat tgagcacttg
     2281 agcatgttca cgctctcgat cttcacctaa caaataatcg agacagcctt ttgataaacc
     2341 tgaaccatgt ctaaagaaat caacgatcat ctggacgtaa ctcccgaagc tgctgatcta
     2401 tggaaatcaa caaatgaagt aacttcactt tgtcaaaagt acctacccct gcaatatcgg
     2461 tattcactgc tttagcaatc tgattcacat tgttgccaat ccgaccaagc tctaaaatta
     2521 atgctcgctc tgtcttgcta tacgaagatt caacaacctt tgttgcttta attttttggt
     2581 catcttctgc tttggaatta tgaattttag tcgctttctc tactgctgct tgtctaagca
     2641 actttgcaat cgaaccataa ggattattgg aatcgagcaa ttgatattct gattctgaaa
     2701 gtctgacttt aaaggttcgt tctcgaactg ctttgctctg tttgttttct tgttcctgtc
     2761 ctgacatttc aatctaccct gctttggctt ttgtaagctt taaattggaa aatctttgat
     2821 ttttctgttt aaagcttttc gggggtatcg ggggtgaagc ccctgatcaa ggtcacagac
     2881 tatcactgtt taacgaagtt aaataggggt agctgtgtat ccttgcttat ttaaaaatgg
     2941 acgagtgaaa ttcaataaaa actaatcaaa tgacacggta tataaactct agtaggtatt
     3001 ggcatgaatc ttgtaaaaac aaatgcgctc aactctttct caccgtcccc acatccctcg
     3061 ctttgctcgg gctgatggtg aacgttgaga gagtttcggc aatactttcc ctaagaggct
     3121 ttgatttaaa aaaatcaaat atcctcttaa gtattgccga ttaacctgta attgggctga
     3181 cggagccacg catcggtcgt atccgcacat ggttttgctg accatgcaga caaggactaa
     3241 aggttttgca ccttctccca cgcgctccct gtcgaagtat ggtggtatct ctgcgaggat
     3301 tactccaaca tgcaacttaa ccaacctata caactacttc caacgctccg cctatgtgag
     3361 atcccctgta agcggtttag ccttgcgact atcgctacga tgcaccagtg acacttattc
     3421 cctccacatg caacgcatat cttccttaaa aaaggcgcac agaagtagat aaagctcgac
     3481 cgttaggtgc ttatttgatg atttaaactg ctgaattaac gaaggacttc aaattgaggc
     3541 ttgtcaaact gttctaattc tttaaaatag atagtggtac tagcctttaa ggtgaatatc
     3601 agtatgagtt gcaagctctg ccgatctttc gttaattgat gttgcaagca tcaaataact
     3661 gtacccatcg aatttaaaac taatggacag atttcgcaag agacctgtcc attatttttt
     3721 ttgattacac ctaagaatta agctgaaact cctattcttt ggactattaa tgtcaaaact
     3781 tcctagattt cataacaagc acatcgaaaa acgtaagtat tcaaatagcc tcctagccaa
     3841 atctatttct tctaaagtac gtttctctaa taaaacattt tttaatgtta atttgagagg
     3901 atcccgccta cgatcatgtt cattcaaaaa tataaaaatt gttttatctg atttttcagg
     3961 agtaatctta aataagaata ggtttacgaa tgttcacttc aaaaagtgcg tattttatgc
     4021 tgctattttt agagattgtc gctttaaaaa ttgcacattt gaaaaatgca tttttattaa
     4081 tacaaataca gaaagcctaa atcactcgat attagactgt tctaaaaaat ataattttta
     4141 cgaaatgaaa attcctgatg acttaaaaga tcaactaaca gaatatagaa atattccaat
     4201 actgcaaaaa aaccgtttac ttcatctaaa agggggaaga atcaataccg caaccttatt
     4261 cattctccta tccaaaatat ccaaatacaa gctgctaaat ggtcttaaaa aaatttatat
     4321 tgaggaacaa agtaaagtaa aaatacttag cactttcaaa ctccttgaag aaataaagaa
     4381 cctagttgac actaacaccg cattcaaaca gaataatggt gctctgcccc atccaatcga
     4441 aaggttggat agttaagaca atcactggga gctcactatt ggatgtaaca attcaattaa
     4501 tagtctaatt actgacattc tgggagggct tactatgtta ggccgcatgg acacaacgca
     4561 ggtcgcattg atacaccaaa ttctagctgc ggcagatgag cgaaatctgc cgctctggat
     4621 cggtgggggc tgggcgatcg atgcacggct agggcgtgta acacgcaagc acgatgatat
     4681 tgatctgact tttcccggcg agaggcgcgg cgagctcgag gcaatagttg aaatgctcgg
     4741 cgggcgcgtc acggaggagt tggactatgg attcttagcg gagatcgggg atgagttact
     4801 tgactgcgaa cctgcttggt gggcagacga agcgtatgaa atcgcggagg ctccgcaggg
     4861 ctcgtgccca gaggcggctg agggtgtcat cgccgggcgg ccagtccgtt gtaacagctg
     4921 ggaggcgatc atctgggatt acttttacta tgccgatgaa gtaccaccag tggactggcc
     4981 tacaaagcac atagagtcct acaggttcgc atgcacctca ctcggggcgg aaaaggttga
     5041 ggtcttgcgt gccgctttca ggtcgcgata tgcggcctaa caattcgtcc aagccgacgc
     5101 cgctttgcgg cgcggcttaa ctcaggtgtt aggaaacact gctaccgtta tcggatttac
     5161 tgctttacta ctaaccttgt taattcaaca tttgcaaaat agtctgacct ttcgattgtt
     5221 tggggtgaaa ttcttatctt aaaaatctaa atattctttt agatctaatg cagattgctc
     5281 gataaaatcg tatgtattaa tacaacgaca gtaaataaat tttgttaaaa cctctaaatc
     5341 attttctaga ttacggttaa aaataatcgg ttcatgatga gcaatcctgt ttcttaattc
     5401 acgtaattga tctgtccaat cgtaaaaaat ttgcctgttt tcaactgttg cattaggaaa
     5461 aatagaatta aaacatccct gccaaatacg ttcttcataa ttagccataa gcatcttttg
     5521 ccagaataca aatttaagct cagggatcat tttactaggc gaatctggga acttctcttt
     5581 agcactatca aacatacttt tagggctata cctccctcta cggctaaggc ttcgaataaa
     5641 agcttcattt tcatgccaac tatttgtttc attacaccta aaagatattt ctattgcctg
     5701 aacaacagca tttctcagaa caacttcaaa ataatttaat aagtaaaaaa acttattgga
     5761 aattattaaa ttacgttcat aaagcatcaa tacatcagat ttttcatatg gttcaccttt
     5821 atctttatag aaattttcat acttagataa gcgtggtata gataaagcct gctctatctt
     5881 aagcactgca tcatttgaca attccaccgt atatacccta atatatagtt gtaagtttga
     5941 gagactgcgg atctaagatc ttccctccca agcgctaaga ttcaaaaaag cctttcatta
     6001 acgtgaaagg ctttttttct ttttttggtc aattaaaagc ttacatattg attgtaattg
     6061 aaatattttc ttgagggac
//
