LOCUS       NZ_CP061663             6079 bp    DNA     circular CON 10-JAN-2024
DEFINITION  Acinetobacter seifertii strain AS31 plasmid pAS31-3, complete
            sequence.
ACCESSION   NZ_CP061663
VERSION     NZ_CP061663.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN16092220
            Assembly: GCF_014722775.1
KEYWORDS    RefSeq.
SOURCE      Acinetobacter seifertii
  ORGANISM  Acinetobacter seifertii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Acinetobacter; Acinetobacter calcoaceticus/baumannii
            complex.
REFERENCE   1  (bases 1 to 6079)
  AUTHORS   Li,L.-H., Yang,Y.-S., Sun,J.-R., Huang,T.-W., Huang,W.-C.,
            Wang,Y.-C., Kuo,T.-H., Kuo,S.-C. and Chen,T.-L.
  TITLE     Clinical and molecular characterization of Acinetobacter seifertii
            in Taiwan
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6079)
  AUTHORS   Chen,F.-J. and Lee,Y.-T.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2020) Department of Emergency Medicine, Taipei
            Veterans General Hospital, No.201, Section 2, Shipai Road, Taipei
            112, Taiwan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 2020
            Assembly Method                   :: CCBGpipe v. 1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 80x
            Sequencing Technology             :: Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/10/2024 01:51:30
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,216
            CDSs (total)                      :: 4,119
            Genes (coding)                    :: 3,982
            CDSs (with protein)               :: 3,982
            Genes (RNA)                       :: 97
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 77 of 137
            Pseudo Genes (incomplete)         :: 61 of 137
            Pseudo Genes (internal stop)      :: 22 of 137
            Pseudo Genes (multiple problems)  :: 19 of 137
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP061663.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..6079
                     /organism="Acinetobacter seifertii"
                     /mol_type="genomic DNA"
                     /strain="AS31"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1530123"
                     /plasmid="pAS31-3"
                     /geo_loc_name="Taiwan"
                     /collection_date="2010/2017"
                     /collected_by="TVGH"
     gene            complement(join(4558..6079,1..293))
                     /locus_tag="IC773_RS20905"
                     /old_locus_tag="IC773_20890"
     CDS             complement(join(4558..6079,1..293))
                     /locus_tag="IC773_RS20905"
                     /old_locus_tag="IC773_20890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000634474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="WP_000634474.1"
                     /translation="MIVDFFRHGSGLSKGCLDYLLGEDREREHAQVLNGDVELTAQLID
                     SSPFAKKYTSGCLSFYEHDLSDQDKQKIMQNFEECLFPGLDKDQYQILWVQHQDKINQD
                     TGETRLELNFVIPNVELSTGKRLQPFYAPVDLDRVDLFKQITNTEHSLYDPDDPEHRQL
                     FLNKKNLPKDIKDFKAQLHQRVYRAVANGDVADRQELVQWLESNQINVTRQVKNSISIE
                     NPYEGAKRPIRLEGEIYEQGFRATGEYRQEVQQRIEEYRGTTSERYRTNVTDYQRQLEH
                     KSQYHCDRYPTVGRENSPEHSKQRPDGREAVKPVSNLAAIEIEPFNAIKRANTEPRTTS
                     PESSGTEKTYHFEYGTDFSSSYFAYSNFLAWSRHQKQVQRDADAKYRDQREANEGRPFE
                     PIGRQFDDQHMQATRQQSTAPMYSDQQERRRMAERLHDSNGVLNDDRIRNTIIENHRRT
                     TATINATTSAIAEATASFRHNANQNYPSLVSTIKDNQERSAKLTASTEIISDNAEAISR
                     ARTKYSKLYRADQWQSPRDEPYHSQQRRNSGENLNHTAFCLVRSSQSAAEFARNIRDIE
                     KNLVQMNERKQEIEKPRPKQDRGMDFGM"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(283..690)
                     /locus_tag="IC773_RS20910"
                     /old_locus_tag="IC773_20895"
     CDS             complement(283..690)
                     /locus_tag="IC773_RS20910"
                     /old_locus_tag="IC773_20895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000016535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_000016535.1"
                     /translation="MSGQEQENKQSKAVRERTFKVRLSESEYQLLDSNNPYGSIAKLLR
                     QAAVEKATKIHNSKAEDDQKIKATKVVESSYSKTERALILELGRIGNNVNQIAKAVNTD
                     IAGVGTFDKVKLLHLLISIDQQLRELRPDDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            1693..2379
                     /locus_tag="IC773_RS20915"
                     /old_locus_tag="IC773_20900"
     CDS             1693..2379
                     /locus_tag="IC773_RS20915"
                     /old_locus_tag="IC773_20900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004986708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="WP_002011386.1"
                     /translation="MSKLPRFHNKHIEKRKYSNSLLAKSISSKVRFSNKTFFNVNLRGS
                     RLRSCSFKNIKIVLSDFSGVILNKNRFTNVHFKKCVFYAAIFRDCRFKNCTFEKCIFIN
                     TNTESLNHSILDCSKKYNFYEMKIPDDLKDQLTEYRNIPILQKNRLLHLKGGRINTATL
                     FILLSKISKYKLLNGLKKIYIEEQSKVKILSTFKLLEEIKNLVDTNTAFKQNNGALPHP
                     IERLDS"
                     /name="pentapeptide repeat-containing protein"
     gene            2470..3003
                     /gene="aadB"
                     /locus_tag="IC773_RS20920"
                     /old_locus_tag="IC773_20905"
     CDS             2470..3003
                     /gene="aadB"
                     /locus_tag="IC773_RS20920"
                     /old_locus_tag="IC773_20905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000381802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase
                     ANT(2'')-Ia"
                     /protein_id="WP_010883707.1"
                     /translation="MDTTQVALIHQILAAADERNLPLWIGGGWAIDARLGRVTRKHDDI
                     DLTFPGERRGELEAIVEMLGGRVTEELDYGFLAEIGDELLDCEPAWWADEAYEIAEAPQ
                     GSCPEAAEGVIAGRPVRCNSWEAIIWDYFYYADEVPPVDWPTKHIESYRFACTSLGAEK
                     VEVLRAAFRSRYAA"
                     /name="aadB"
     gene            complement(3162..3830)
                     /locus_tag="IC773_RS20925"
                     /old_locus_tag="IC773_20910"
     CDS             complement(3162..3830)
                     /locus_tag="IC773_RS20925"
                     /old_locus_tag="IC773_20910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000423610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Abi family protein"
                     /protein_id="WP_000423610.1"
                     /translation="MELSNDAVLKIEQALSIPRLSKYENFYKDKGEPYEKSDVLMLYER
                     NLIISNKFFYLLNYFEVVLRNAVVQAIEISFRCNETNSWHENEAFIRSLSRRGRYSPKS
                     MFDSAKEKFPDSPSKMIPELKFVFWQKMLMANYEERIWQGCFNSIFPNATVENRQIFYD
                     WTDQLRELRNRIAHHEPIIFNRNLENDLEVLTKFIYCRCINTYDFIEQSALDLKEYLDF
                     "
                     /name="Abi family protein"
     gene            complement(3964..4449)
                     /locus_tag="IC773_RS20930"
                     /old_locus_tag="IC773_20915"
     CDS             complement(3964..4449)
                     /locus_tag="IC773_RS20930"
                     /old_locus_tag="IC773_20915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005200081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_051064109.1"
                     /translation="MNNNCVVVLTARGKTRILKEGGSQSWRLNAHNAGKCDYLVCVQNR
                     KQSWGEPEADHHTAFLVGKISSISQSQEEDADGRWIINISEYADISIPNMWDGNRNPVS
                     YSSLEEMNIDLNELKFTKLNEDNSSKTLDKISIPEAKRLLANYFDVPQENISITINM"
                     /name="hypothetical protein"
     ARG             2471..3000
                     /name="ant(2'')-Ia"
                     /gene="ant(2'')-Ia"
                     /gene_name="aminoglycoside nucleotidyltransferase
                     ANT(2'')-Ia"
                     /tool="amrfinderplus"
                     /drug_class="AMINOGLYCOSIDE"
                     /identity="98.31"
                     /coverage="100.0"
     ARG             2459..3003
                     /name="ANT(2'')-Ia"
                     /gene="ANT(2'')-Ia"
                     /gene_name="ANT(2'')"
                     /tool="rgi"
                     /drug_class="aminoglycoside antibiotic"
                     /identity="97.74"
                     /coverage="102.26"
     Typing          complement(4..293)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="97.0"
                     /evalue="5.42e-59"
     Typing          complement(4562..6078)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 tggtgctgta cccataaaat ttgatattgg tctttatcta gtcccgggaa taaacattcc
       61 tcaaaatttt gcatgatttt ctgtttatcc tggtcagata aatcatgctc ataaaaggac
      121 aaacaaccac tggtatattt tttggcaaaa ggactgctat caatgagttg tgctgtcagt
      181 tctacatcac cattgagcac ttgagcatgt tcacgctctc gatcttcacc taacaaataa
      241 tcgagacagc cttttgataa acctgaacca tgtctaaaga aatcaacgat catctggacg
      301 taactcccga agctgctgat ctatggaaat caacaaatga agtaacttca ctttgtcaaa
      361 agtacctacc cctgcaatat cggtattcac tgctttagca atctgattca cattgttgcc
      421 aatccgacca agctctaaaa ttaatgctcg ctctgtcttg ctatacgaag attcaacaac
      481 ctttgttgct ttaatttttt ggtcatcttc tgctttggaa ttatgaattt tagtcgcttt
      541 ctctactgct gcttgtctaa gcaactttgc aatcgaacca taaggattat tggaatcgag
      601 caattgatat tctgattctg aaagtctgac tttaaaggtt cgttctcgaa ctgctttgct
      661 ctgtttgttt tcttgttcct gtcctgacat ttcaatctac cctgctttgg cttttgtaag
      721 ctttaaattg gaaaatcttt gatttttctg tttaaagctt ttcgggggta tcgggggtga
      781 agcccctgat caaggtcaca gactatcact gtttaacgaa gttaaatagg ggtagctgtg
      841 tatccttgct tatttaaaaa tggacgagtg aaattcaata aaaactaatc aaatgacacg
      901 gtatataaac tctagtaggt attggcatga atcttgtaaa aacaaatgcg ctcaactctt
      961 tctcaccgtc cccacatccc tcgctttgct cgggctgatg gtgaacgttg agagagtttc
     1021 ggcaatactt tccctaagag gctttgattt aaaaaaatca aatatcctct taagtattgc
     1081 cgattaacct gtaattgggc tgacggagcc acgcatcggt cgtatccgca catggttttg
     1141 ctgaccatgc agacaaggac taaaggtttt gcaccttctc ccacgcgctc cctgtcgaag
     1201 tatggtggta tctctgcgag gattactcca acatgcaact taaccaacct atacaactac
     1261 ttccaacgct ccgcctatgt gagatcccct gtaagcggtt tagccttgcg actatcgcta
     1321 cgatgcacca gtgacactta ttccctccac atgcaacgca tatcttcctt aaaaaaggcg
     1381 cacagaagta gataaagctc gaccgttagg tgcttatttg atgatttaaa ctgctgaatt
     1441 aacgaaggac ttcaaattga ggcttgtcaa actgttctaa ttctttaaaa tagatagtgg
     1501 tactagcctt taaggtgaat atcagtatga gttgcaagct ctgccgatct ttcgttaatt
     1561 gatgttgcaa gcatcaaata actgtaccca tcgaatttaa aactaatgga cagatttcgc
     1621 aagagacctg tccattattt tttttgatta cacctaagaa ttaagctgaa actcctattc
     1681 tttggactat taatgtcaaa acttcctaga tttcataaca agcacatcga aaaacgtaag
     1741 tattcaaata gcctcctagc caaatctatt tcttctaaag tacgtttctc taataaaaca
     1801 ttttttaatg ttaatttgag aggatcccgc ctacgatcat gttcattcaa aaatataaaa
     1861 attgttttat ctgatttttc aggagtaatc ttaaataaga ataggtttac gaatgttcac
     1921 ttcaaaaagt gcgtatttta tgctgctatt tttagagatt gtcgctttaa aaattgcaca
     1981 tttgaaaaat gcatttttat taatacaaat acagaaagcc taaatcactc gatattagac
     2041 tgttctaaaa aatataattt ttacgaaatg aaaattcctg atgacttaaa agatcaacta
     2101 acagaatata gaaatattcc aatactgcaa aaaaaccgtt tacttcatct aaaaggggga
     2161 agaatcaata ccgcaacctt attcattctc ctatccaaaa tatccaaata caagctgcta
     2221 aatggtctta aaaaaattta tattgaggaa caaagtaaag taaaaatact tagcactttc
     2281 aaactccttg aagaaataaa gaacctagtt gacactaaca ccgcattcaa acagaataat
     2341 ggtgctctgc cccatccaat cgaaaggttg gatagttaag acaatcactg ggagctcact
     2401 attggatgta acaattcaat taatagtcta attactgaca ttctgggagg gcttactatg
     2461 ttaggccgca tggacacaac gcaggtcgca ttgatacacc aaattctagc tgcggcagat
     2521 gagcgaaatc tgccgctctg gatcggtggg ggctgggcga tcgatgcacg gctagggcgt
     2581 gtaacacgca agcacgatga tattgatctg acttttcccg gcgagaggcg cggcgagctc
     2641 gaggcaatag ttgaaatgct cggcgggcgc gtcacggagg agttggacta tggattctta
     2701 gcggagatcg gggatgagtt acttgactgc gaacctgctt ggtgggcaga cgaagcgtat
     2761 gaaatcgcgg aggctccgca gggctcgtgc ccagaggcgg ctgagggtgt catcgccggg
     2821 cggccagtcc gttgtaacag ctgggaggcg atcatctggg attactttta ctatgccgat
     2881 gaagtaccac cagtggactg gcctacaaag cacatagagt cctacaggtt cgcatgcacc
     2941 tcactcgggg cggaaaaggt tgaggtcttg cgtgccgctt tcaggtcgcg atatgcggcc
     3001 taacaattcg tccaagccga cgccgctttg cggcgcggct taactcaggt gttaggaaac
     3061 actgctaccg ttatcggatt tactgcttta ctactaacct tgttaattca acatttgcaa
     3121 aatagtctga cctttcgatt gtttggggtg aaattcttat cttaaaaatc taaatattct
     3181 tttagatcta atgcagattg ctcgataaaa tcgtatgtat taatacaacg acagtaaata
     3241 aattttgtta aaacctctaa atcattttct agattacggt taaaaataat cggttcatga
     3301 tgagcaatcc tgtttcttaa ttcacgtaat tgatctgtcc aatcgtaaaa aatttgcctg
     3361 ttttcaactg ttgcattagg aaaaatagaa ttaaaacatc cctgccaaat acgttcttca
     3421 taattagcca taagcatctt ttgccagaat acaaatttaa gctcagggat cattttacta
     3481 ggcgaatctg ggaacttctc tttagcacta tcaaacatac ttttagggct atacctccct
     3541 ctacggctaa ggcttcgaat aaaagcttca ttttcatgcc aactatttgt ttcattacac
     3601 ctaaaagata tttctattgc ctgaacaaca gcatttctca gaacaacttc aaaataattt
     3661 aataagtaaa aaaacttatt ggaaattatt aaattacgtt cataaagcat caatacatca
     3721 gatttttcat atggttcacc tttatcttta tagaaatttt catacttaga taagcgtggt
     3781 atagataaag cctgctctat cttaagcact gcatcatttg acaattccac cgtatatacc
     3841 ctaatatata gttgtaagtt tgagagactg cggatctaag atcttccctc ccaagcgcta
     3901 agattcaaaa aagcctttca ttaacgtgaa aggctttttt tctttttttg gtcaattaaa
     3961 agcttacata ttgattgtaa ttgaaatatt ttcttgaggg acatcaaaat aattagctaa
     4021 tagtctctta gcttcaggaa tagatatttt atccagtgtt ttagaagaat tatcttcatt
     4081 taatttggta aatttcagct cattcaaatc gatattcatc tcttctaatg aactataaga
     4141 aactggattc cgattaccat cccacatatt gggaatagat atatcagcat attcggagat
     4201 atttattatc catctaccat ctgcatcttc ttcttgagat tggctgatac tagatatttt
     4261 gccaactaaa aatgcagtgt gatgatcagc ctctggttct ccccaagatt gtttcctatt
     4321 ttgcacacaa actagataat cgcactttcc tgcattatga gcatttagtc tccaagactg
     4381 actaccacct tcttttaaaa ttctagtctt tccacgagca gttagtacta ctacacagtt
     4441 attattcata acaccaccaa aatatataaa caagcctata ctacattcaa tatagctaaa
     4501 tttaaacatt agtacaagct aatactaaga aataaacacc aacatactag aaaataatta
     4561 catcccaaaa tccatacctc gatcttgctt aggtcgaggt ttttctatct cttgtttacg
     4621 ctcattcatc tggacgagat ttttttcaat atctcggatg tttcgagcaa attctgctgc
     4681 gctttggcta cttctgacga gacagaatgc tgtgtggttg agattctctc cagaatttct
     4741 tcgttgttgg ctgtgataag gctcatctct cggactttgc cattgatctg ctcggtatag
     4801 cttgctgtac ttcgttctag ctcgtgaaat tgcttctgca ttgtcgctaa tgatttctgt
     4861 gcttgctgta agctttgctg atcgctcttg attgtctttg attgtgctga caaggcttgg
     4921 ataattttgg tttgcattgt gtctaaagct tgctgttgct tctgcaattg cacttgttgt
     4981 tgcattaatt gttgctgttg ttcttcgatg attctcaata attgtgtttc taattcggtc
     5041 atcgtttaat accccattgg aatcatgtag ccgttctgcc attcgcctgc gttcttgttg
     5101 atctgaatac ataggcgctg tgctttgctg tctggtcgct tgcatgtgct gatcgtcaaa
     5161 ctgcctcccg atcggttcaa atggtcgacc ctcatttgct tctcgttgat cacggtattt
     5221 agcgtctgca tctcgctgta cttgtttttg gtgacgagac caagcgagaa aattgctata
     5281 agcaaaataa ctgctactga aatcagtccc atattcaaag tggtaagttt tttctgtacc
     5341 gcttgattct gggcttgttg ttcttggctc tgtatttgct cgtttaattg cgttaaatgg
     5401 ctcaatttcg attgcagcaa ggtttgatac aggcttaact gcttctcgac cgtctgggcg
     5461 ttgttttgaa tgctctgggc tattttctcg tccgactgtt ggatagcggt cgcagtgata
     5521 ttggctctta tgttcaagct gtctctgata gtctgtgacg tttgttcgat accgctcaga
     5581 agttgttcct ctgtattctt caattcgttg ttgtacttct tgtctatatt cgccagtagc
     5641 tctaaagcct tgctcataaa tctcgccctc taaacggatg ggacgttttg cgccctcata
     5701 tgggttttca atggatatgg agttttttac ttgtcgagtg acattgattt gattcgattc
     5761 aagccattgc accaattctt gccgatcggc aacatcacca ttggcaaccg ctcggtaaac
     5821 acgttgatgt aactgcgctt taaaatcctt aatatctttg ggtaggttct ttttatttaa
     5881 aaatagctgt cgatgctcag gatcatcggg gtcatacagt gaatgttcgg tattagtgat
     5941 ctgcttaaat agatcgactc gatctaaatc gactggcgca tagaatggct gtaggcgttt
     6001 gcctgttgaa agctcaacat taggaatcac aaaattaagc tcaagccttg tctcacctgt
     6061 gtcctgatta attttgtct
//
