LOCUS       NZ_CP061659             3855 bp    DNA     circular CON 04-SEP-2023
DEFINITION  Acinetobacter seifertii strain AS33 plasmid pAS33-4, complete
            sequence.
ACCESSION   NZ_CP061659
VERSION     NZ_CP061659.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN16092221
            Assembly: GCF_014722535.1
KEYWORDS    RefSeq.
SOURCE      Acinetobacter seifertii
  ORGANISM  Acinetobacter seifertii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Acinetobacter; Acinetobacter calcoaceticus/baumannii
            complex.
REFERENCE   1  (bases 1 to 3855)
  AUTHORS   Li,L.-H., Yang,Y.-S., Sun,J.-R., Huang,T.-W., Huang,W.-C.,
            Wang,Y.-C., Kuo,T.-H., Kuo,S.-C. and Chen,T.-L.
  TITLE     Clinical and molecular characterization of Acinetobacter seifertii
            in Taiwan
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3855)
  AUTHORS   Chen,F.-J. and Lee,Y.-T.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2020) Department of Emergency Medicine, Taipei
            Veterans General Hospital, No.201, Section 2, Shipai Road, Taipei
            112, Taiwan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 2020
            Assembly Method                   :: CCBGpipe v. 1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 80x
            Sequencing Technology             :: Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 09/03/2023 22:11:58
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,287
            Genes (coding)                    :: 3,903
            CDSs (with protein)               :: 3,903
            Genes (RNA)                       :: 97
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 323 of 384
            Pseudo Genes (incomplete)         :: 59 of 384
            Pseudo Genes (internal stop)      :: 24 of 384
            Pseudo Genes (multiple problems)  :: 18 of 384
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP061659.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3855
                     /organism="Acinetobacter seifertii"
                     /mol_type="genomic DNA"
                     /strain="AS33"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1530123"
                     /plasmid="pAS33-4"
                     /geo_loc_name="Taiwan"
                     /collection_date="2010/2017"
                     /collected_by="TVGH"
     gene            join(3691..3855,1..798)
                     /locus_tag="IC774_RS21690"
                     /old_locus_tag="IC774_21680"
     CDS             join(3691..3855,1..798)
                     /locus_tag="IC774_RS21690"
                     /old_locus_tag="IC774_21680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004977197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_071212525.1"
                     /translation="MLNKTQFSDDFYEKLPKKPYCSDDLGRGVIIRPKRTAIQKPYIQH
                     NPPCLVSSLVFDIDRQDAYFAWSDANLPTPTWIAKNRQNGHAHIGYMLLAPVCTTHRAK
                     QNVIQYLAKIEQAYSLALGADRGYTGLITKNPCHGTWENHTFGVQPYDLNYLADFVELQ
                     ELKTDLKEVSGLGRNCMMFDTVRFWAYEAIRAFISGSPDSWYAEVLKVAINANGAFLEP
                     LPYSEVKATAKSIARWVWRNHTEAHAKFIERQTVKGRKGGLSSDSSHGGKARSQQYSDM
                     RQEALKLHIQGIKQKDIALQLNVSDRTIRTWIKKRKVQA"
                     /name="replication initiation protein"
     gene            1063..1347
                     /locus_tag="IC774_RS22140"
     CDS             1063..1347
                     /locus_tag="IC774_RS22140"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_223861191.1"
                     /translation="MKRKDTWRPLCYADVFHDLVQGQAPETPKSKGKSMTAIKRNKVYK
                     VRFSDAEVQKIKDANILNIAKYLRDTSLAQIDATQGNAKKRIRKTTTQN"
                     /name="hypothetical protein"
     gene            1359..1508
                     /gene="mobC"
                     /locus_tag="IC774_RS22145"
     CDS             1359..1508
                     /gene="mobC"
                     /locus_tag="IC774_RS22145"
                     /inference="COORDINATES: protein motif:HMM:NF017524.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="WP_223861193.1"
                     /translation="MLELSRIGGNINQIAKAINTDIASSRPLDAARLLHLLIGIDQTLK
                     ELKK"
                     /name="mobC"
     gene            1505..3406
                     /locus_tag="IC774_RS21700"
                     /old_locus_tag="IC774_21690"
     CDS             1505..3406
                     /locus_tag="IC774_RS21700"
                     /old_locus_tag="IC774_21690"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="WP_144624569.1"
                     /translation="MIVQFFRYGNGLSKGPLDYLLGKDRDRDYAKVLQGDVSEVSGLID
                     TSPYAKKYTSGCLSFYEHDLSDQDKQKIMQDFEQALFPGMDQSQYRVLWIEHQDKLNEE
                     TGERRLELNFLIPNVEILTGQRLQPYYDKADRPRIDLFKKITNYEYQLHDADDPLYRQA
                     VTTAKNLPKTVNEIKETLDIEATRAVEGGLITDRQSMKKWLLDLGLEVTRETKKSLSIK
                     NPNDDEKARPIRLTGAIYEQDFRLTETSQQLTIAASERYRREAKQRNQSDIQRYAEYSA
                     KRSTELEQQYRQHQNVYTAETERSNERDIAADRPDYSSNQEAAATRHDSPIARTTTADQ
                     TATSELAAVEQLEPRNSPSSQIKENPYYIEYSLDFTGMYNAYQQYLSGVRQQEQIQRHH
                     RDGEQSPVVKKPRGEPEYSEVWDREKMELVRPDRPESAAVQQQHANSAGNQLNESRSTV
                     IADYRAATAAAQRATEAARASLAAHSGTEQNHRRIRELQQQTSEALQPVERERKRADQY
                     HQETTESEAIRGFIARLGEQLKTAITEPFRAISDWVKSKERDQRTIANDDLYRNREADS
                     TIERTASKETRLSATVSRKIRGFDQTNIFKALDELDRRRELQLEQQRKNDRSYSPRF"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     Typing          1506..3400
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="92.575"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 ttcgatatag accgccaaga cgcttatttt gcgtggtctg acgcaaattt accgacccca
       61 acatggatag ccaaaaatcg ccaaaatgga catgcacata ttggctatat gcttttagct
      121 ccagtgtgta ccacgcatcg agcaaagcag aatgtcatac agtaccttgc caagattgag
      181 caggcgtata gcttggcgtt aggtgcagat cgtggctata cggggttaat cactaaaaat
      241 ccatgtcacg gaacatggga aaatcatacc tttggcgttc aaccatacga cctaaattat
      301 ttagccgatt ttgtagagct tcaggaacta aaaaccgact taaaagaagt ttcagggctt
      361 gggcgtaact gtatgatgtt cgataccgtt cgtttttggg cgtatgaagc gattagagcg
      421 tttatttcgg ggtcgcctga tagttggtat gcggaagtgc taaaagtcgc tataaacgca
      481 aatggtgcgt ttttagagcc attgccatat tcagaggtaa aggcaaccgc taagtctatt
      541 gctcgttggg tatggcgcaa tcacacagaa gcacatgcca aatttatcga gcgtcagact
      601 gtcaaagggc gtaaaggcgg tctaagcagt gattccagtc atggcggtaa ggctaggtca
      661 cagcaataca gtgatatgcg tcaggaagcc ttaaaactgc atatacaggg cataaagcag
      721 aaagatatag ccctacaatt gaatgtttcg gatagaacaa tcagaacgtg gataaaaaag
      781 cggaaagtgc aagcctaaat cagatatcag ccccctaggg tgcttttttc ccatttcatt
      841 tttaaaaatt attattaaag ggaatgagca acggctgtgg gcaaagtaaa accctgtgat
      901 ctaaacctgt gtgcaactca acgtataacg gctttatcgt tatgcggagt tgtgcatagt
      961 gtttaggcac agatcaggtt ttacggtcgc ttgcgattgt ccatagtctt gctatgacct
     1021 gcttaatctt aattgaatta cgtctagggg tctgctatcg taatgaaacg caaggataca
     1081 tggagacccc tctgctacgc agatgtgttc catgaccttg ttcaggggca agcccccgaa
     1141 acccccaaaa gcaaaggcaa aagcatgaca gcgatcaaac gtaataaggt ttacaaagtc
     1201 cgattttctg atgcagaggt acagaaaatc aaagatgcca atatcctaaa tattgccaaa
     1261 tatctaaggg atacttcgct tgcccaaatt gatgcaaccc aaggcaacgc caaaaaaaga
     1321 atcaggaaaa ccactacaca aaattagacc gtgacttttt gcttgagcta tcccgaattg
     1381 ggggcaacat caatcagatt gcaaaggcaa taaatactga tattgcaagc agtcgtcccc
     1441 ttgatgctgc aaggcttttg cacctgttaa tagggattga tcagacctta aaggaattga
     1501 aaaaatgatt gttcaattct ttagatatgg gaacggttta agtaaaggtc cactggatta
     1561 tttacttggt aaagaccgtg atcgagatta tgcaaaagtc ctacagggcg atgtttccga
     1621 agtatcagga ttaattgata cgtcacctta tgcaaaaaaa tatacatccg gctgcctaag
     1681 tttttatgag catgatttat ctgaccagga taaacaaaaa atcatgcagg attttgagca
     1741 agccttattc cctggcatgg atcagagcca gtaccgtgtc ttgtggattg agcatcaaga
     1801 caaattaaac gaagaaacag gggagcgaag attagagctt aacttcctaa tccctaatgt
     1861 tgaaatcctt acagggcaac gtttacagcc ttattatgat aaagctgatc gtcctcgtat
     1921 tgatctgttt aagaagatta caaactatga atatcaactg catgatgcag atgaccccct
     1981 ctatcgccaa gccgttacaa cggcaaaaaa cttgcctaaa actgttaatg aaatcaagga
     2041 aacgcttgat attgaagcaa cgagagccgt tgaaggtgga ctaattacag atcggcagag
     2101 catgaaaaaa tggctattgg atcttggtct tgaagtcacc agagaaacca agaaaagcct
     2161 atccattaaa aatccgaatg atgacgaaaa agcccgcccg ataaggctta caggtgcaat
     2221 ctatgagcaa gattttagac ttacagaaac gagccaacaa cttactatcg cagcatcaga
     2281 acgatataga cgagaagcta aacagcgaaa tcagtcagat attcaaagat atgcggaata
     2341 cagtgcaaag cgaagcacag agcttgagca gcagtataga cagcaccaaa acgtatatac
     2401 agcagaaaca gagcgaagca acgaacgaga tattgcagca gatcgaccag actacagctc
     2461 aaaccaagaa gcagcagcaa caaggcatga cagccctatt gcaagaacaa caacagcaga
     2521 tcaaacagct acaagcgaac ttgcagcagt tgaacagctt gagccaagaa acagcccaag
     2581 cagccaaatt aaagaaaacc cttattacat cgaatatagc cttgatttca ctggcatgta
     2641 taacgcttat cagcagtatt tgtctggggt acgtcagcaa gagcaaatac agcgacatca
     2701 cagagatgga gaacaaagtc cagtggttaa aaagccaagg ggcgagcctg aatattcaga
     2761 agtgtgggac agagaaaaaa tggaactggt gcgtccagat cgacccgaaa gcgcagcagt
     2821 acagcaacaa catgcgaata gtgcaggaaa ccaattaaat gaatctcgaa gcacagttat
     2881 tgcagattat cgagcagcaa cagcagcagc tcaaagagca acagaagcag caagagcaag
     2941 ccttgcagct cattcaggca cagaacagaa ccatagacga attagagaac tacaacagca
     3001 aactagtgaa gcactgcaac cagttgaacg agaaagaaaa cgagctgatc aatatcatca
     3061 agaaactaca gaatccgaag ctatcagagg atttattgca aggcttggag agcagcttaa
     3121 aacagcgatt acagagccat ttagagcaat tagtgactgg gttaaatcta aagaacgaga
     3181 tcaacgaact attgccaacg atgatctata cagaaatcga gaagcagaca gcaccattga
     3241 acgaacagct tcaaaggaaa ctagactcag tgcaacagta tcacgaaaaa ttcgagggtt
     3301 tgatcaaaca aatatcttca aggctttaga tgaactggac agacgtagag agttgcaact
     3361 ggagcaacaa cgtaaaaatg acaggagtta ttcacctaga ttttgatact ttttttctgc
     3421 ggagcattgc ccttgcaatg ctgtcctcga aaaaaaagca tcttcaaagg tcacatattt
     3481 gcgacatttt gtcgcaatat tttgttttga aaatcagtca tttgacagca attcaaaaag
     3541 gtgtaatatt ttcatcaggc gaaaaaaagc aggtttggcg acccgctttt ttctctttgt
     3601 taaacaaacg cttaaagcat ccgtaaaact caaattcgat acttgtattt tactgatgtt
     3661 ttgagccaat gcaacacttg gacgtgtttc atgctcaata aaacgcaatt ttcagacgat
     3721 ttttacgaaa aactcccaaa aaaaccgtat tgctcggatg atttagggcg tggggtgatt
     3781 attcgcccaa aacgtaccgc aatccagaag ccatacattc agcataatcc cccttgtctt
     3841 gttagctcgt tggtg
//
