LOCUS       NZ_CP061658             6078 bp    DNA     circular CON 04-SEP-2023
DEFINITION  Acinetobacter seifertii strain AS33 plasmid pAS33-3, complete
            sequence.
ACCESSION   NZ_CP061658
VERSION     NZ_CP061658.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN16092221
            Assembly: GCF_014722535.1
KEYWORDS    RefSeq.
SOURCE      Acinetobacter seifertii
  ORGANISM  Acinetobacter seifertii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Acinetobacter; Acinetobacter calcoaceticus/baumannii
            complex.
REFERENCE   1  (bases 1 to 6078)
  AUTHORS   Li,L.-H., Yang,Y.-S., Sun,J.-R., Huang,T.-W., Huang,W.-C.,
            Wang,Y.-C., Kuo,T.-H., Kuo,S.-C. and Chen,T.-L.
  TITLE     Clinical and molecular characterization of Acinetobacter seifertii
            in Taiwan
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6078)
  AUTHORS   Chen,F.-J. and Lee,Y.-T.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2020) Department of Emergency Medicine, Taipei
            Veterans General Hospital, No.201, Section 2, Shipai Road, Taipei
            112, Taiwan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 2020
            Assembly Method                   :: CCBGpipe v. 1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 80x
            Sequencing Technology             :: Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 09/03/2023 22:11:58
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,287
            Genes (coding)                    :: 3,903
            CDSs (with protein)               :: 3,903
            Genes (RNA)                       :: 97
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 323 of 384
            Pseudo Genes (incomplete)         :: 59 of 384
            Pseudo Genes (internal stop)      :: 24 of 384
            Pseudo Genes (multiple problems)  :: 18 of 384
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP061658.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..6078
                     /organism="Acinetobacter seifertii"
                     /mol_type="genomic DNA"
                     /strain="AS33"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1530123"
                     /plasmid="pAS33-3"
                     /geo_loc_name="Taiwan"
                     /collection_date="2010/2017"
                     /collected_by="TVGH"
     gene            join(5745..6078,1..1481)
                     /locus_tag="IC774_RS21660"
                     /old_locus_tag="IC774_21650"
     CDS             join(5745..6078,1..1481)
                     /locus_tag="IC774_RS21660"
                     /old_locus_tag="IC774_21650"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="WP_000634474.1"
                     /translation="MIVDFFRHGSGLSKGCLDYLLGEDREREHAQVLNGDVELTAQLID
                     SSPFAKKYTSGCLSFYEHDLSDQDKQKIMQNFEECLFPGLDKDQYQILWVQHQDKINQD
                     TGETRLELNFVIPNVELSTGKRLQPFYAPVDLDRVDLFKQITNTEHSLYDPDDPEHRQL
                     FLNKKNLPKDIKDFKAQLHQRVYRAVANGDVADRQELVQWLESNQINVTRQVKNSISIE
                     NPYEGAKRPIRLEGEIYEQGFRATGEYRQEVQQRIEEYRGTTSERYRTNVTDYQRQLEH
                     KSQYHCDRYPTVGRENSPEHSKQRPDGREAVKPVSNLAAIEIEPFNAIKRANTEPRTTS
                     PESSGTEKTYHFEYGTDFSSSYFAYSNFLAWSRHQKQVQRDADAKYRDQREANEGRPFE
                     PIGRQFDDQHMQATRQQSTAPMYSDQQERRRMAERLHDSNGVLNDDRIRNTIIENHRRT
                     TATINATTSAIAEATASFRHNANQNYPSLVSTIKDNQERSAKLTASTEIISDNAEAISR
                     ARTKYSKLYRADQWQSPRDEPYHSQQRRNSGENLNHTAFCLVRSSQSAAEFARNIRDIE
                     KNLVQMNERKQEIEKPRPKQDRGMDFGM"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            1590..2075
                     /locus_tag="IC774_RS21665"
                     /old_locus_tag="IC774_21655"
     CDS             1590..2075
                     /locus_tag="IC774_RS21665"
                     /old_locus_tag="IC774_21655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005200081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_051064109.1"
                     /translation="MNNNCVVVLTARGKTRILKEGGSQSWRLNAHNAGKCDYLVCVQNR
                     KQSWGEPEADHHTAFLVGKISSISQSQEEDADGRWIINISEYADISIPNMWDGNRNPVS
                     YSSLEEMNIDLNELKFTKLNEDNSSKTLDKISIPEAKRLLANYFDVPQENISITINM"
                     /name="hypothetical protein"
     gene            2209..2877
                     /locus_tag="IC774_RS21670"
                     /old_locus_tag="IC774_21660"
     CDS             2209..2877
                     /locus_tag="IC774_RS21670"
                     /old_locus_tag="IC774_21660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000423610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Abi family protein"
                     /protein_id="WP_000423610.1"
                     /translation="MELSNDAVLKIEQALSIPRLSKYENFYKDKGEPYEKSDVLMLYER
                     NLIISNKFFYLLNYFEVVLRNAVVQAIEISFRCNETNSWHENEAFIRSLSRRGRYSPKS
                     MFDSAKEKFPDSPSKMIPELKFVFWQKMLMANYEERIWQGCFNSIFPNATVENRQIFYD
                     WTDQLRELRNRIAHHEPIIFNRNLENDLEVLTKFIYCRCINTYDFIEQSALDLKEYLDF
                     "
                     /name="Abi family protein"
     gene            complement(3036..3569)
                     /gene="aadB"
                     /locus_tag="IC774_RS21675"
                     /old_locus_tag="IC774_21665"
     CDS             complement(3036..3569)
                     /gene="aadB"
                     /locus_tag="IC774_RS21675"
                     /old_locus_tag="IC774_21665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000381802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase
                     ANT(2'')-Ia"
                     /protein_id="WP_010883707.1"
                     /translation="MDTTQVALIHQILAAADERNLPLWIGGGWAIDARLGRVTRKHDDI
                     DLTFPGERRGELEAIVEMLGGRVTEELDYGFLAEIGDELLDCEPAWWADEAYEIAEAPQ
                     GSCPEAAEGVIAGRPVRCNSWEAIIWDYFYYADEVPPVDWPTKHIESYRFACTSLGAEK
                     VEVLRAAFRSRYAA"
                     /name="aadB"
     gene            complement(3660..4346)
                     /locus_tag="IC774_RS21680"
                     /old_locus_tag="IC774_21670"
     CDS             complement(3660..4346)
                     /locus_tag="IC774_RS21680"
                     /old_locus_tag="IC774_21670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004986708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="WP_002011386.1"
                     /translation="MSKLPRFHNKHIEKRKYSNSLLAKSISSKVRFSNKTFFNVNLRGS
                     RLRSCSFKNIKIVLSDFSGVILNKNRFTNVHFKKCVFYAAIFRDCRFKNCTFEKCIFIN
                     TNTESLNHSILDCSKKYNFYEMKIPDDLKDQLTEYRNIPILQKNRLLHLKGGRINTATL
                     FILLSKISKYKLLNGLKKIYIEEQSKVKILSTFKLLEEIKNLVDTNTAFKQNNGALPHP
                     IERLDS"
                     /name="pentapeptide repeat-containing protein"
     gene            5348..5755
                     /locus_tag="IC774_RS21685"
                     /old_locus_tag="IC774_21675"
     CDS             5348..5755
                     /locus_tag="IC774_RS21685"
                     /old_locus_tag="IC774_21675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000016535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_000016535.1"
                     /translation="MSGQEQENKQSKAVRERTFKVRLSESEYQLLDSNNPYGSIAKLLR
                     QAAVEKATKIHNSKAEDDQKIKATKVVESSYSKTERALILELGRIGNNVNQIAKAVNTD
                     IAGVGTFDKVKLLHLLISIDQQLRELRPDDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     ARG             complement(3040..3569)
                     /name="ant(2'')-Ia"
                     /gene="ant(2'')-Ia"
                     /gene_name="aminoglycoside nucleotidyltransferase
                     ANT(2'')-Ia"
                     /tool="amrfinderplus"
                     /drug_class="AMINOGLYCOSIDE"
                     /identity="98.31"
                     /coverage="100.0"
     ARG             complement(3037..3581)
                     /name="ANT(2'')-Ia"
                     /gene="ANT(2'')-Ia"
                     /gene_name="ANT(2'')"
                     /tool="rgi"
                     /drug_class="aminoglycoside antibiotic"
                     /identity="97.74"
                     /coverage="102.26"
     Typing          4..1478
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          5746..6077
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="97.0"
                     /evalue="9.8e-69"
ORIGIN
        1 ttaattttgt gattcctaat gttgagcttt caacaggcaa acgcctacag ccattctatg
       61 cgccagtcga tttagatcga gtcgatctat ttaagcagat cactaatacc gaacattcac
      121 tgtatgaccc cgatgatcct gagcatcgac agctattttt aaataaaaag aacctaccca
      181 aagatattaa ggattttaaa gcgcagttac atcaacgtgt ttaccgagcg gttgccaatg
      241 gtgatgttgc cgatcggcaa gaattggtgc aatggcttga atcgaatcaa atcaatgtca
      301 ctcgacaagt aaaaaactcc atatccattg aaaacccata tgagggcgca aaacgtccca
      361 tccgtttaga gggcgagatt tatgagcaag gctttagagc tactggcgaa tatagacaag
      421 aagtacaaca acgaattgaa gaatacagag gaacaacttc tgagcggtat cgaacaaacg
      481 tcacagacta tcagagacag cttgaacata agagccaata tcactgcgac cgctatccaa
      541 cagtcggacg agaaaatagc ccagagcatt caaaacaacg cccagacggt cgagaagcag
      601 ttaagcctgt atcaaacctt gctgcaatcg aaattgagcc atttaacgca attaaacgag
      661 caaatacaga gccaagaaca acaagcccag aatcaagcgg tacagaaaaa acttaccact
      721 ttgaatatgg gactgatttc agtagcagtt attttgctta tagcaatttt ctcgcttggt
      781 ctcgtcacca aaaacaagta cagcgagatg cagacgctaa ataccgtgat caacgagaag
      841 caaatgaggg tcgaccattt gaaccgatcg ggaggcagtt tgacgatcag cacatgcaag
      901 cgaccagaca gcaaagcaca gcgcctatgt attcagatca acaagaacgc aggcgaatgg
      961 cagaacggct acatgattcc aatggggtat taaacgatga ccgaattaga aacacaatta
     1021 ttgagaatca tcgaagaaca acagcaacaa ttaatgcaac aacaagtgca attgcagaag
     1081 caacagcaag ctttagacac aatgcaaacc aaaattatcc aagccttgtc agcacaatca
     1141 aagacaatca agagcgatca gcaaagctta cagcaagcac agaaatcatt agcgacaatg
     1201 cagaagcaat ttcacgagct agaacgaagt acagcaagct ataccgagca gatcaatggc
     1261 aaagtccgag agatgagcct tatcacagcc aacaacgaag aaattctgga gagaatctca
     1321 accacacagc attctgtctc gtcagaagta gccaaagcgc agcagaattt gctcgaaaca
     1381 tccgagatat tgaaaaaaat ctcgtccaga tgaatgagcg taaacaagag atagaaaaac
     1441 ctcgacctaa gcaagatcga ggtatggatt ttgggatgta attattttct agtatgttgg
     1501 tgtttatttc ttagtattag cttgtactaa tgtttaaatt tagctatatt gaatgtagta
     1561 taggcttgtt tatatatttt ggtggtgtta tgaataataa ctgtgtagta gtactaactg
     1621 ctcgtggaaa gactagaatt ttaaaagaag gtggtagtca gtcttggaga ctaaatgctc
     1681 ataatgcagg aaagtgcgat tatctagttt gtgtgcaaaa taggaaacaa tcttggggag
     1741 aaccagaggc tgatcatcac actgcatttt tagttggcaa aatatctagt atcagccaat
     1801 ctcaagaaga agatgcagat ggtagatgga taataaatat ctccgaatat gctgatatat
     1861 ctattcccaa tatgtgggat ggtaatcgga atccagtttc ttatagttca ttagaagaga
     1921 tgaatatcga tttgaatgag ctgaaattta ccaaattaaa tgaagataat tcttctaaaa
     1981 cactggataa aatatctatt cctgaagcta agagactatt agctaattat tttgatgtcc
     2041 ctcaagaaaa tatttcaatt acaatcaata tgtaagcttt taattgacca aaaaaaagaa
     2101 aaaagccttt cacgttaatg aaaggctttt ttgaatctta gcgcttggga gggaagatct
     2161 tagatccgca gtctctcaaa cttacaacta tatattaggg tatatacggt ggaattgtca
     2221 aatgatgcag tgcttaagat agagcaggct ttatctatac cacgcttatc taagtatgaa
     2281 aatttctata aagataaagg tgaaccatat gaaaaatctg atgtattgat gctttatgaa
     2341 cgtaatttaa taatttccaa taagtttttt tacttattaa attattttga agttgttctg
     2401 agaaatgctg ttgttcaggc aatagaaata tcttttaggt gtaatgaaac aaatagttgg
     2461 catgaaaatg aagcttttat tcgaagcctt agccgtagag ggaggtatag ccctaaaagt
     2521 atgtttgata gtgctaaaga gaagttccca gattcgccta gtaaaatgat ccctgagctt
     2581 aaatttgtat tctggcaaaa gatgcttatg gctaattatg aagaacgtat ttggcaggga
     2641 tgttttaatt ctatttttcc taatgcaaca gttgaaaaca ggcaaatttt ttacgattgg
     2701 acagatcaat tacgtgaatt aagaaacagg attgctcatc atgaaccgat tatttttaac
     2761 cgtaatctag aaaatgattt agaggtttta acaaaattta tttactgtcg ttgtattaat
     2821 acatacgatt ttatcgagca atctgcatta gatctaaaag aatatttaga tttttaagat
     2881 aagaatttca ccccaaacaa tcgaaaggtc agactatttt gcaaatgttg aattaacaag
     2941 gttagtagta aagcagtaaa tccgataacg gtagcagtgt ttcctaacac ctgagttaag
     3001 ccgcgccgca aagcggcgtc ggcttggacg aattgttagg ccgcatatcg cgacctgaaa
     3061 gcggcacgca agacctcaac cttttccgcc ccgagtgagg tgcatgcgaa cctgtaggac
     3121 tctatgtgct ttgtaggcca gtccactggt ggtacttcat cggcatagta aaagtaatcc
     3181 cagatgatcg cctcccagct gttacaacgg actggccgcc cggcgatgac accctcagcc
     3241 gcctctgggc acgagccctg cggagcctcc gcgatttcat acgcttcgtc tgcccaccaa
     3301 gcaggttcgc agtcaagtaa ctcatccccg atctccgcta agaatccata gtccaactcc
     3361 tccgtgacgc gcccgccgag catttcaact attgcctcga gctcgccgcg cctctcgccg
     3421 ggaaaagtca gatcaatatc atcgtgcttg cgtgttacac gccctagccg tgcatcgatc
     3481 gcccagcccc caccgatcca gagcggcaga tttcgctcat ctgccgcagc tagaatttgg
     3541 tgtatcaatg cgacctgcgt tgtgtccatg cggcctaaca tagtaagccc tcccagaatg
     3601 tcagtaatta gactattaat tgaattgtta catccaatag tgagctccca gtgattgtct
     3661 taactatcca acctttcgat tggatggggc agagcaccat tattctgttt gaatgcggtg
     3721 ttagtgtcaa ctaggttctt tatttcttca aggagtttga aagtgctaag tatttttact
     3781 ttactttgtt cctcaatata aattttttta agaccattta gcagcttgta tttggatatt
     3841 ttggatagga gaatgaataa ggttgcggta ttgattcttc ccccttttag atgaagtaaa
     3901 cggttttttt gcagtattgg aatatttcta tattctgtta gttgatcttt taagtcatca
     3961 ggaattttca tttcgtaaaa attatatttt ttagaacagt ctaatatcga gtgatttagg
     4021 ctttctgtat ttgtattaat aaaaatgcat ttttcaaatg tgcaattttt aaagcgacaa
     4081 tctctaaaaa tagcagcata aaatacgcac tttttgaagt gaacattcgt aaacctattc
     4141 ttatttaaga ttactcctga aaaatcagat aaaacaattt ttatattttt gaatgaacat
     4201 gatcgtaggc gggatcctct caaattaaca ttaaaaaatg ttttattaga gaaacgtact
     4261 ttagaagaaa tagatttggc taggaggcta tttgaatact tacgtttttc gatgtgcttg
     4321 ttatgaaatc taggaagttt tgacattaat agtccaaaga ataggagttt cagcttaatt
     4381 cttaggtgta atcaaaaaaa taatggacag gtctcttgcg aaatctgtcc attagtttta
     4441 aattcgatgg gtacagttat ttgatgcttg caacatcaat taacgaaaga tcggcagagc
     4501 ttgcaactca tactgatatt caccttaaag gctagtacca ctatctattt taaagaatta
     4561 gaacagtttg acaagcctca atttgaagtc cttcgttaat tcagcagttt aaatcatcaa
     4621 ataagcacct aacggtcgag ctttatctac ttctgtgcgc cttttttaag gaagatatgc
     4681 gttgcatgtg gagggaataa gtgtcactgg tgcatcgtag cgatagtcgc aaggctaaac
     4741 cgcttacagg ggatctcaca taggcggagc gttggaagta gttgtatagg ttggttaagt
     4801 tgcatgttgg agtaatcctc gcagagatac caccatactt cgacagggag cgcgtgggag
     4861 aaggtgcaaa acctttagtc cttgtctgca tggtcagcaa aaccatgtgc ggatacgacc
     4921 gatgcgtggc tccgtcagcc caattacagg ttaatcggca atacttaaga ggatatttga
     4981 tttttttaaa tcaaagcctc ttagggaaag tattgccgaa actctctcaa cgttcaccat
     5041 cagcccgagc aaagcgaggg atgtggggac ggtgagaaag agttgagcgc atttgttttt
     5101 acaagattca tgccaatacc tactagagtt tatataccgt gtcatttgat tagtttttat
     5161 tgaatttcac tcgtccattt ttaaataagc aaggatacac agctacccct atttaacttc
     5221 gttaaacagt gatagtctgt gaccttgatc aggggcttca cccccgatac ccccgaaaag
     5281 ctttaaacag aaaaatcaaa gattttccaa tttaaagctt acaaaagcca aagcagggta
     5341 gattgaaatg tcaggacagg aacaagaaaa caaacagagc aaagcagttc gagaacgaac
     5401 ctttaaagtc agactttcag aatcagaata tcaattgctc gattccaata atccttatgg
     5461 ttcgattgca aagttgctta gacaagcagc agtagagaaa gcgactaaaa ttcataattc
     5521 caaagcagaa gatgaccaaa aaattaaagc aacaaaggtt gttgaatctt cgtatagcaa
     5581 gacagagcga gcattaattt tagagcttgg tcggattggc aacaatgtga atcagattgc
     5641 taaagcagtg aataccgata ttgcaggggt aggtactttt gacaaagtga agttacttca
     5701 tttgttgatt tccatagatc agcagcttcg ggagttacgt ccagatgatc gttgatttct
     5761 ttagacatgg ttcaggttta tcaaaaggct gtctcgatta tttgttaggt gaagatcgag
     5821 agcgtgaaca tgctcaagtg ctcaatggtg atgtagaact gacagcacaa ctcattgata
     5881 gcagtccttt tgccaaaaaa tataccagtg gttgtttgtc cttttatgag catgatttat
     5941 ctgaccagga taaacagaaa atcatgcaaa attttgagga atgtttattc ccgggactag
     6001 ataaagacca atatcaaatt ttatgggtac agcaccaaga caaaattaat caggacacag
     6061 gtgagacaag gcttgagc
//
