LOCUS       NZ_CP061653             6077 bp    DNA     circular CON 04-SEP-2023
DEFINITION  Acinetobacter seifertii strain AS34 plasmid pAS34-3, complete
            sequence.
ACCESSION   NZ_CP061653
VERSION     NZ_CP061653.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN16092222
            Assembly: GCF_014722275.1
KEYWORDS    RefSeq.
SOURCE      Acinetobacter seifertii
  ORGANISM  Acinetobacter seifertii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Acinetobacter; Acinetobacter calcoaceticus/baumannii
            complex.
REFERENCE   1  (bases 1 to 6077)
  AUTHORS   Li,L.-H., Yang,Y.-S., Sun,J.-R., Huang,T.-W., Huang,W.-C.,
            Wang,Y.-C., Kuo,T.-H., Kuo,S.-C. and Chen,T.-L.
  TITLE     Clinical and molecular characterization of Acinetobacter seifertii
            in Taiwan
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6077)
  AUTHORS   Chen,F.-J. and Lee,Y.-T.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2020) Department of Emergency Medicine, Taipei
            Veterans General Hospital, No.201, Section 2, Shipai Road, Taipei
            112, Taiwan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 2020
            Assembly Method                   :: CCBGpipe v. 1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 80x
            Sequencing Technology             :: Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 09/03/2023 22:19:01
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,241
            CDSs (total)                      :: 4,144
            Genes (coding)                    :: 3,703
            CDSs (with protein)               :: 3,703
            Genes (RNA)                       :: 97
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 441
            CDSs (without protein)            :: 441
            Pseudo Genes (ambiguous residues) :: 0 of 441
            Pseudo Genes (frameshifted)       :: 376 of 441
            Pseudo Genes (incomplete)         :: 63 of 441
            Pseudo Genes (internal stop)      :: 23 of 441
            Pseudo Genes (multiple problems)  :: 18 of 441
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP061653.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..6077
                     /organism="Acinetobacter seifertii"
                     /mol_type="genomic DNA"
                     /strain="AS34"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1530123"
                     /plasmid="pAS34-3"
                     /geo_loc_name="Taiwan"
                     /collection_date="2010/2017"
                     /collected_by="TVGH"
     gene            complement(join(4541..6077,1..278))
                     /locus_tag="IC775_RS20895"
                     /old_locus_tag="IC775_20885"
     CDS             complement(join(4541..6077,1..278))
                     /locus_tag="IC775_RS20895"
                     /old_locus_tag="IC775_20885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000634474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="WP_000634474.1"
                     /translation="MIVDFFRHGSGLSKGCLDYLLGEDREREHAQVLNGDVELTAQLID
                     SSPFAKKYTSGCLSFYEHDLSDQDKQKIMQNFEECLFPGLDKDQYQILWVQHQDKINQD
                     TGETRLELNFVIPNVELSTGKRLQPFYAPVDLDRVDLFKQITNTEHSLYDPDDPEHRQL
                     FLNKKNLPKDIKDFKAQLHQRVYRAVANGDVADRQELVQWLESNQINVTRQVKNSISIE
                     NPYEGAKRPIRLEGEIYEQGFRATGEYRQEVQQRIEEYRGTTSERYRTNVTDYQRQLEH
                     KSQYHCDRYPTVGRENSPEHSKQRPDGREAVKPVSNLAAIEIEPFNAIKRANTEPRTTS
                     PESSGTEKTYHFEYGTDFSSSYFAYSNFLAWSRHQKQVQRDADAKYRDQREANEGRPFE
                     PIGRQFDDQHMQATRQQSTAPMYSDQQERRRMAERLHDSNGVLNDDRIRNTIIENHRRT
                     TATINATTSAIAEATASFRHNANQNYPSLVSTIKDNQERSAKLTASTEIISDNAEAISR
                     ARTKYSKLYRADQWQSPRDEPYHSQQRRNSGENLNHTAFCLVRSSQSAAEFARNIRDIE
                     KNLVQMNERKQEIEKPRPKQDRGMDFGM"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(268..675)
                     /locus_tag="IC775_RS20900"
                     /old_locus_tag="IC775_20890"
     CDS             complement(268..675)
                     /locus_tag="IC775_RS20900"
                     /old_locus_tag="IC775_20890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000016535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_000016535.1"
                     /translation="MSGQEQENKQSKAVRERTFKVRLSESEYQLLDSNNPYGSIAKLLR
                     QAAVEKATKIHNSKAEDDQKIKATKVVESSYSKTERALILELGRIGNNVNQIAKAVNTD
                     IAGVGTFDKVKLLHLLISIDQQLRELRPDDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            1678..2364
                     /locus_tag="IC775_RS20905"
                     /old_locus_tag="IC775_20895"
     CDS             1678..2364
                     /locus_tag="IC775_RS20905"
                     /old_locus_tag="IC775_20895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004986708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="WP_002011386.1"
                     /translation="MSKLPRFHNKHIEKRKYSNSLLAKSISSKVRFSNKTFFNVNLRGS
                     RLRSCSFKNIKIVLSDFSGVILNKNRFTNVHFKKCVFYAAIFRDCRFKNCTFEKCIFIN
                     TNTESLNHSILDCSKKYNFYEMKIPDDLKDQLTEYRNIPILQKNRLLHLKGGRINTATL
                     FILLSKISKYKLLNGLKKIYIEEQSKVKILSTFKLLEEIKNLVDTNTAFKQNNGALPHP
                     IERLDS"
                     /name="pentapeptide repeat-containing protein"
     gene            2455..2988
                     /gene="aadB"
                     /locus_tag="IC775_RS20910"
                     /old_locus_tag="IC775_20900"
     CDS             2455..2988
                     /gene="aadB"
                     /locus_tag="IC775_RS20910"
                     /old_locus_tag="IC775_20900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000381802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase
                     ANT(2'')-Ia"
                     /protein_id="WP_010883707.1"
                     /translation="MDTTQVALIHQILAAADERNLPLWIGGGWAIDARLGRVTRKHDDI
                     DLTFPGERRGELEAIVEMLGGRVTEELDYGFLAEIGDELLDCEPAWWADEAYEIAEAPQ
                     GSCPEAAEGVIAGRPVRCNSWEAIIWDYFYYADEVPPVDWPTKHIESYRFACTSLGAEK
                     VEVLRAAFRSRYAA"
                     /name="aadB"
     gene            complement(3146..3814)
                     /locus_tag="IC775_RS20915"
                     /old_locus_tag="IC775_20905"
     CDS             complement(3146..3814)
                     /locus_tag="IC775_RS20915"
                     /old_locus_tag="IC775_20905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000423610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Abi family protein"
                     /protein_id="WP_000423610.1"
                     /translation="MELSNDAVLKIEQALSIPRLSKYENFYKDKGEPYEKSDVLMLYER
                     NLIISNKFFYLLNYFEVVLRNAVVQAIEISFRCNETNSWHENEAFIRSLSRRGRYSPKS
                     MFDSAKEKFPDSPSKMIPELKFVFWQKMLMANYEERIWQGCFNSIFPNATVENRQIFYD
                     WTDQLRELRNRIAHHEPIIFNRNLENDLEVLTKFIYCRCINTYDFIEQSALDLKEYLDF
                     "
                     /name="Abi family protein"
     gene            complement(3947..4432)
                     /locus_tag="IC775_RS20920"
                     /old_locus_tag="IC775_20910"
     CDS             complement(3947..4432)
                     /locus_tag="IC775_RS20920"
                     /old_locus_tag="IC775_20910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005200081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_051064109.1"
                     /translation="MNNNCVVVLTARGKTRILKEGGSQSWRLNAHNAGKCDYLVCVQNR
                     KQSWGEPEADHHTAFLVGKISSISQSQEEDADGRWIINISEYADISIPNMWDGNRNPVS
                     YSSLEEMNIDLNELKFTKLNEDNSSKTLDKISIPEAKRLLANYFDVPQENISITINM"
                     /name="hypothetical protein"
     ARG             2456..2985
                     /name="ant(2'')-Ia"
                     /gene="ant(2'')-Ia"
                     /gene_name="aminoglycoside nucleotidyltransferase
                     ANT(2'')-Ia"
                     /tool="amrfinderplus"
                     /drug_class="AMINOGLYCOSIDE"
                     /identity="98.31"
                     /coverage="100.0"
     ARG             2444..2988
                     /name="ANT(2'')-Ia"
                     /gene="ANT(2'')-Ia"
                     /gene_name="ANT(2'')"
                     /tool="rgi"
                     /drug_class="aminoglycoside antibiotic"
                     /identity="97.74"
                     /coverage="102.26"
     Typing          complement(4..278)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="97.0"
                     /evalue="1.1699999999999999e-54"
     Typing          complement(4545..6076)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 aaaatttgat attggtcttt atctagtccc gggaataaac attcctcaaa attttgcatg
       61 attttctgtt tatcctggtc agataaatca tgctcataaa aggacaaaca accactggta
      121 tattttttgg caaaaggact gctatcaatg agttgtgctg tcagttctac atcaccattg
      181 agcacttgag catgttcacg ctctcgatct tcacctaaca aataatcgag acagcctttt
      241 gataaacctg aaccatgtct aaagaaatca acgatcatct ggacgtaact cccgaagctg
      301 ctgatctatg gaaatcaaca aatgaagtaa cttcactttg tcaaaagtac ctacccctgc
      361 aatatcggta ttcactgctt tagcaatctg attcacattg ttgccaatcc gaccaagctc
      421 taaaattaat gctcgctctg tcttgctata cgaagattca acaacctttg ttgctttaat
      481 tttttggtca tcttctgctt tggaattatg aattttagtc gctttctcta ctgctgcttg
      541 tctaagcaac tttgcaatcg aaccataagg attattggaa tcgagcaatt gatattctga
      601 ttctgaaagt ctgactttaa aggttcgttc tcgaactgct ttgctctgtt tgttttcttg
      661 ttcctgtcct gacatttcaa tctaccctgc tttggctttt gtaagcttta aattggaaaa
      721 tctttgattt ttctgtttaa agcttttcgg gggtatcggg ggtgaagccc ctgatcaagg
      781 tcacagacta tcactgttta acgaagttaa ataggggtag ctgtgtatcc ttgcttattt
      841 aaaaatggac gagtgaaatt caataaaaac taatcaaatg acacggtata taaactctag
      901 taggtattgg catgaatctt gtaaaaacaa atgcgctcaa ctctttctca ccgtccccac
      961 atccctcgct ttgctcgggc tgatggtgaa cgttgagaga gtttcggcaa tactttccct
     1021 aagaggcttt gatttaaaaa aatcaaatat cctcttaagt attgccgatt aacctgtaat
     1081 tgggctgacg gagccacgca tcggtcgtat ccgcacatgg ttttgctgac catgcagaca
     1141 aggactaaag gttttgcacc ttctcccacg cgctccctgt cgaagtatgg tggtatctct
     1201 gcgaggatta ctccaacatg caacttaacc aacctataca actacttcca acgctccgcc
     1261 tatgtgagat cccctgtaag cggtttagcc ttgcgactat cgctacgatg caccagtgac
     1321 acttattccc tccacatgca acgcatatct tccttaaaaa aggcgcacag aagtagataa
     1381 agctcgaccg ttaggtgctt atttgatgat ttaaactgct gaattaacga aggacttcaa
     1441 attgaggctt gtcaaactgt tctaattctt taaaatagat agtggtacta gcctttaagg
     1501 tgaatatcag tatgagttgc aagctctgcc gatctttcgt taattgatgt tgcaagcatc
     1561 aaataactgt acccatcgaa tttaaaacta atggacagat ttcgcaagag acctgtccat
     1621 tatttttttt gattacacct aagaattaag ctgaaactcc tattctttgg actattaatg
     1681 tcaaaacttc ctagatttca taacaagcac atcgaaaaac gtaagtattc aaatagcctc
     1741 ctagccaaat ctatttcttc taaagtacgt ttctctaata aaacattttt taatgttaat
     1801 ttgagaggat cccgcctacg atcatgttca ttcaaaaata taaaaattgt tttatctgat
     1861 ttttcaggag taatcttaaa taagaatagg tttacgaatg ttcacttcaa aaagtgcgta
     1921 ttttatgctg ctatttttag agattgtcgc tttaaaaatt gcacatttga aaaatgcatt
     1981 tttattaata caaatacaga aagcctaaat cactcgatat tagactgttc taaaaaatat
     2041 aatttttacg aaatgaaaat tcctgatgac ttaaaagatc aactaacaga atatagaaat
     2101 attccaatac tgcaaaaaaa ccgtttactt catctaaaag ggggaagaat caataccgca
     2161 accttattca ttctcctatc caaaatatcc aaatacaagc tgctaaatgg tcttaaaaaa
     2221 atttatattg aggaacaaag taaagtaaaa atacttagca ctttcaaact ccttgaagaa
     2281 ataaagaacc tagttgacac taacaccgca ttcaaacaga ataatggtgc tctgccccat
     2341 ccaatcgaaa ggttggatag ttaagacaat cactgggagc tcactattgg atgtaacaat
     2401 tcaattaata gtctaattac tgacattctg ggagggctta ctatgttagg ccgcatggac
     2461 acaacgcagg tcgcattgat acaccaaatt ctagctgcgg cagatgagcg aaatctgccg
     2521 ctctggatcg gtgggggctg ggcgatcgat gcacggctag ggcgtgtaac acgcaagcac
     2581 gatgatattg atctgacttt tcccggcgag aggcgcggcg agctcgaggc aatagttgaa
     2641 atgctcggcg ggcgcgtcac ggaggagttg gactatggat tcttagcgga gatcggggat
     2701 gagttacttg actgcgaacc tgcttggtgg gcagacgaag cgtatgaaat cgcggaggct
     2761 ccgcagggct cgtgcccaga ggcggctgag ggtgtcatcg ccgggcggcc agtccgttgt
     2821 aacagctggg aggcgatcat ctgggattac ttttactatg ccgatgaagt accaccagtg
     2881 gactggccta caaagcacat agagtcctac aggttcgcat gcacctcact cggggcggaa
     2941 aaggttgagg tcttgcgtgc cgctttcagg tcgcgatatg cggcctaaca attcgtcaag
     3001 ccgacgccgc tttgcggcgc ggcttaactc aggtgttagg aaacactgct accgttatcg
     3061 gatttactgc tttactacta accttgttaa ttcaacattt gcaaaatagt ctgacctttc
     3121 gattgtttgg ggtgaaattc ttatcttaaa aatctaaata ttcttttaga tctaatgcag
     3181 attgctcgat aaaatcgtat gtattaatac aacgacagta aataaatttt gttaaaacct
     3241 ctaaatcatt ttctagatta cggttaaaaa taatcggttc atgatgagca atcctgtttc
     3301 ttaattcacg taattgatct gtccaatcgt aaaaaatttg cctgttttca actgttgcat
     3361 taggaaaaat agaattaaaa catccctgcc aaatacgttc ttcataatta gccataagca
     3421 tcttttgcca gaatacaaat ttaagctcag ggatcatttt actaggcgaa tctgggaact
     3481 tctctttagc actatcaaac atacttttag ggctatacct ccctctacgg ctaaggcttc
     3541 gaataaaagc ttcattttca tgccaactat ttgtttcatt acacctaaaa gatatttcta
     3601 ttgcctgaac aacagcattt ctcagaacaa cttcaaaata atttaataag taaaaaaact
     3661 tattggaaat tattaaatta cgttcataaa gcatcaatac atcagatttt tcatatggtt
     3721 cacctttatc tttatagaaa ttttcatact tagataagcg tggtatagat aaagcctgct
     3781 ctatcttaag cactgcatca tttgacaatt ccaccgtata taccctaata tatagttgta
     3841 agtttgagag actgcggatc taagatcttc cctcccaagc gctaagattc aaaaaagcct
     3901 ttcattaacg tgaaaggctt tttttctttt ttggtcaatt aaaagcttac atattgattg
     3961 taattgaaat attttcttga gggacatcaa aataattagc taatagtctc ttagcttcag
     4021 gaatagatat tttatccagt gttttagaag aattatcttc atttaatttg gtaaatttca
     4081 gctcattcaa atcgatattc atctcttcta atgaactata agaaactgga ttccgattac
     4141 catcccacat attgggaata gatatatcag catattcgga gatatttatt atccatctac
     4201 catctgcatc ttcttcttga gattggctga tactagatat tttgccaact aaaaatgcag
     4261 tgtgatgatc agcctctggt tctccccaag attgtttcct attttgcaca caaactagat
     4321 aatcgcactt tcctgcatta tgagcattta gtctccaaga ctgactacca ccttctttta
     4381 aaattctagt ctttccacga gcagttagta ctactacaca gttattattc ataacaccac
     4441 caaaatatat aaacaagcct atactacatt caatatagct aaatttaaac attagtacaa
     4501 gctaatacta agaaataaac accaacatac tagaaaataa ttacatccca aaatccatac
     4561 ctcgatcttg cttaggtcga ggtttttcta tctcttgttt acgctcattc atctggacga
     4621 gatttttttc aatatctcgg atgtttcgag caaattctgc tgcgctttgg ctacttctga
     4681 cgagacagaa tgctgtgtgg ttgagattct ctccagaatt tcttcgttgt tggctgtgat
     4741 aaggctcatc tctcggactt tgccattgat ctgctcggta tagcttgctg tacttcgttc
     4801 tagctcgtga aattgcttct gcattgtcgc taatgatttc tgtgcttgct gtaagctttg
     4861 ctgatcgctc ttgattgtct ttgattgtgc tgacaaggct tggataattt tggtttgcat
     4921 tgtgtctaaa gcttgctgtt gcttctgcaa ttgcacttgt tgttgcatta attgttgctg
     4981 ttgttcttcg atgattctca ataattgtgt ttctaattcg gtcatcgttt aataccccat
     5041 tggaatcatg tagccgttct gccattcgcc tgcgttcttg ttgatctgaa tacataggcg
     5101 ctgtgctttg ctgtctggtc gcttgcatgt gctgatcgtc aaactgcctc ccgatcggtt
     5161 caaatggtcg accctcattt gcttctcgtt gatcacggta tttagcgtct gcatctcgct
     5221 gtacttgttt ttggtgacga gaccaagcga gaaaattgct ataagcaaaa taactgctac
     5281 tgaaatcagt cccatattca aagtggtaag ttttttctgt accgcttgat tctgggcttg
     5341 ttgttcttgg ctctgtattt gctcgtttaa ttgcgttaaa tggctcaatt tcgattgcag
     5401 caaggtttga tacaggctta actgcttctc gaccgtctgg gcgttgtttt gaatgctctg
     5461 ggctattttc tcgtccgact gttggatagc ggtcgcagtg atattggctc ttatgttcaa
     5521 gctgtctctg atagtctgtg acgtttgttc gataccgctc agaagttgtt cctctgtatt
     5581 cttcaattcg ttgttgtact tcttgtctat attcgccagt agctctaaag ccttgctcat
     5641 aaatctcgcc ctctaaacgg atgggacgtt ttgcgccctc atatgggttt tcaatggata
     5701 tggagttttt tacttgtcga gtgacattga tttgattcga ttcaagccat tgcaccaatt
     5761 cttgccgatc ggcaacatca ccattggcaa ccgctcggta aacacgttga tgtaactgcg
     5821 ctttaaaatc cttaatatct ttgggtaggt tctttttatt taaaaatagc tgtcgatgct
     5881 caggatcatc ggggtcatac agtgaatgtt cggtattagt gatctgctta aatagatcga
     5941 ctcgatctaa atcgactggc gcatagaatg gctgtaggcg tttgcctgtt gaaagctcaa
     6001 cattaggaat cacaaaatta agctcaagcc ttgtctcacc tgtgtcctga ttaattttgt
     6061 cttggtgctg tacccat
//
